Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0009249

Physical Location & Seq

Genomic Coordinates Forward (+)

15362704 .. 15369178

Gene Structure 6475 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG151400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2589 bp

ATGACATGTGCAGCTTGCGTGAACTCTGTTGAAGGTATTCTGAAAGGGCTTCCTGGGGTCAAAAGAGCTGTAGTAGCTTTAGCTACTTCACTAGGGGAAGTGGAGTATGATCCTACTGTAATTAGTAAAGATGACATAGTCAATGCCATTGAAGATGCTGGTTTTGAAGCATCTTTTGTACAGAGTAGTGAGCAGGATAAAATTATACTGGGAGTTGCTGGCATATTCAGTGAGATAGATGCACAGGTTTTAGAAGCCATAATCAGCAACTTGAAAGGGGTGAGACATTTTCGTCTTGACAGGATTTCAAGAGAACTTGAAATCCTCTTTGATCCTGAAGTTGTCACTTCTAGATCCTTGGTTGATGGGATTGAGGGGGCTAGCAATGGGAAGTTCAAACTTCAAGTAGCAAACCCTTATACAAGAATGACTTGCAAAGATGTTGATGAAGCCGCAAATATGTTTCGGCTTTTCATTTCCAGCCTGGTTCTCAGTGTTCCTGTCTTTCTCATTCGAGTGGTCTGCCCTCACATTCCACTTCTGTATTCATTATTGCTCTGGAGATGTGGGCCTTTCGAAATGGGTGATTGGTTGAAGTGGGCATTGGTGAGTGTTGTTCAATTTGTAATTGGAAAGCGCTTCTACATTGCAGCTGCAAGAGCCCTACGAAATGGTTCAACTAACATGGATGTTCTGGTCGCGTTGGGAACTTCGGCCTCTTACTTCTACTCTGTTTGTGCACTTCTATATGGTGCAATCACTGGGTTTTGGTCTCCGACATACTTTGAAACAAGTGCCATGCTAATAACATTTGTACTATTGGGGAAGTATCTGGAATGTCTTGCAAAAGGAAAGACATCAGATGCCATCAAAAAGTTGATAGAACTCACTCCAGCTACAGCTTTGTTGCTTGTTAAAGATAAAGGTGGGAGATGTGTTGGAGAGAGGGAAATTGATGCTTTGCTCATTCAGCCTGGAGATACGTTAAAAGTTCTTCCTGGTACAAAAGTTCCTGCTGATGGTATGGTTGTGTGGGGTTCAAGTTATGTAAATGAGAGTATGGTAACTGGGGAAGCTATACCTGTTTCAAAAGAAGTCAACACGTTGGTGATTGGAGGAACAATAAATTTACATGGTGCCCTTCACGTACAAGTTACAAAAGTTGGATCTGATACAGTTTTACATCAAATAATTAATTTGGTCGAGACTGCTCAAATGTCCAAAGCTCCTATTCAGAAATTTGCTGATTTTGTTGCAAGCATTTTTGTTCCTACTGTAGTTGTATTGTCATTGTTGACATTTTTGGGTTGGTATGTTGCTGGAGCCTTTGGAGCTTACCCAGAACAGTGGCTCCCTGAAAATGGAAATCACTTTGTTTTTGCCCTTATGTTTTCCATATCAGTTGTGGTCATTGCATGCCCCTGTGCACTTGGCTTGGCAACACCAACAGCTGTCATGGTCGCAACAGGGGTTGGTGCTAATAATGGTGTGCTGATAAAAGGAGGAGATGCTTTGGAAAGAGCTCAAAAGGTTAAGTACGTGATATTTGATAAAACAGGCACCCTAACCCAAGGAAAGGCTACAGTTACTGCTGTAAAAATTTTCACAGGAATGGATCGTGGAGATTTTCTTAAGTTGGTGGCATCTGCAGAGGCTAGCAGTGAACACCCACTAGGAAAAGCAATAGTTGAATATGCACGCCATTTCCACTTCTTTGATGAGCCTTCTGCAACCAATGATGCAACAAACCAAAGCAAGGAGCCCCTGATCTCTGGATGGCTTTTTGATGTCTCAGATTTCTCTGCTTTGCCAGGTAGAGGCATCCAGTGCTCAATAAATGGAAAACTTATTCTGGTTGGAAATAGGAAATTGATGACTGAAAGCGGAATCGACATTCCAACCCATGTTGAGGACTTTGTAGTAGAGCTGGAAGAAAGTGCAAAGACAGGCATACTTGTTGCATATGAAGGCAATTTAGTTGGTGTATTGGGGGTTGCCGACCCACTAAAAAGAGAAGCTGCAATAGTCATAGAGGGACTTGGTAAGATGGGTGTCATACCAGTCATGGTTACAGGAGATAATTGGAGGACAGCACAGGCTGTTGCAAAGGAGGTTGGCATTAAAGATGTGCGAGCGGAGGTAATGCCGGCTGGAAAAGCTGATGTTGTACGTTCATTCCAAAAAGATGGAAGCATAGTTGCAATGGTTGGTGATGGAATCAACGACTCTCCTGCCCTAGCTGCTTCTGATGTTGGTATGGCAATAGGGGCAGGGACAGATATTGCAATTGAAGCAGCTGACTATGTATTGATGAGAAATAACTTGGAAGATGTGATCACAGCCGTTGATCTCTCGAGAAAGACCTTCACTCGTATCCGATTGAATTATGTGTTTGCCATGGCCTACAATGTGATTGCAATACCTATTGCCGCAGGGGTTTTCTTTCCTTCACTGGGGATCATGCTGCCGCCATGGGTAGCCGGTGCATGCATGGCTATGTCATCTGTTAGTGTTGTATGCTCCTCTTTGCTCCTTAGGAGATACAGAAAACCCCGACTTACCGCCATACTGGAAATAGTTGTAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0012505 GO:0016020 GO:0023052 GO:0031410 GO:0031982 GO:0031984 GO:0032870 GO:0035556 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071369 GO:0071495 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

862

Amino Acids

92.41

Weight (kDa)

5.55

Isoelectric Point (pI)

26.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	1 - 56	4e-13	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	297 - 402	2e-24	P-type ATPase actuator domain
Hydrolase	PF00702	511 - 749	1.2e-29	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0009249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44790
fragaria_vesca	FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710
malus_domestica	MD05G1247900.v1.1 MD10G1228800.v1.1
prunus_persica	Prupe.4G115900_v2.0.a1 Prupe.4G115900_v2.0.a1
pyrus_communis	pycom05g22420 pycom10g19240
rosa_chinensis	RchiOBHm_Chr5g0020701
rosa_laevigata	RLG00000032554
rosa_multiflora	Rmu_sc0005607.1_g000032
rosa_roxburghii	Rroxscaffold_1G00057600
rosa_rugosa	Rorug05G0062500
rosa_samantha	Rh5AG152200 Rh5BG151900 Rh5CG163300 Rh5DG151400
rosa_wichuraiana	Rw5G013490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	291
AccB1I	GGYRCC	2 cut(s)	1136, 1559
AccBSI	CCGCTC	1 cut(s)	2135
AccII	CGCG	1 cut(s)	701
AciI	CCGC	6 cut(s)	453, 1884, 2135, 2430, 2468, 2562
AclWI	GGATC	6 cut(s)	104, 326, 348, 1174, 1623, 2465
AcsI	RAATTY	3 cut(s)	1126, 1238, 1599
AcuI	CTGAAG	1 cut(s)	357
AfaI	GTAC	6 cut(s)	180, 816, 1003, 1149, 1538, 2169
AfeI	AGCGCT	1 cut(s)	638
AfiI	CCNNNNNNNGG	7 cut(s)	1019, 1361, 1576, 1756, 1817, 1909, 2453
AflII	CTTAAG	1 cut(s)	1631
AflIII	ACRYGT	2 cut(s)	5, 1101
AjnI	CCWGG	5 cut(s)	52, 483, 973, 997, 1810
AjuI	GAANNNNNNNTTGG	2 cut(s)	587, 619
AleI	CACNNNNGTG	1 cut(s)	1106
AloI	GAACNNNNNNTCC	4 cut(s)	306, 338, 1335, 1367
Alw21I	GWGCWC	4 cut(s)	742, 1429, 1525, 1832
Alw26I	GTCTC	4 cut(s)	277, 777, 1199, 1795
Alw44I	GTGCAC	2 cut(s)	738, 1425
AlwI	GGATC	6 cut(s)	104, 326, 348, 1174, 1623, 2465
AlwNI	CAGNNNCTG	1 cut(s)	1589
Ama87I	CYCGRG	1 cut(s)	2353
Aor51HI	AGCGCT	1 cut(s)	638
AoxI	GGCC	3 cut(s)	569, 714, 2400
ApaLI	GTGCAC	2 cut(s)	738, 1425
ApeKI	GCWGC	7 cut(s)	11, 650, 653, 2018, 2240, 2294, 2464
ApoI	RAATTY	3 cut(s)	1126, 1238, 1599
AseI	ATTAAT	1 cut(s)	1194
Asp700I	GAANNNNTTC	2 cut(s)	36, 673
AspLEI	GCGC	1 cut(s)	639
AspS9I	GGNCC	1 cut(s)	569
AsuHPI	GGTGA	5 cut(s)	292, 596, 619, 1120, 2222
AsuII	TTCGAA	1 cut(s)	576
AsuNHI	GCTAGC	2 cut(s)	380, 1655
AvaI	CYCGRG	1 cut(s)	2353
AxyI	CCTNAGG	1 cut(s)	2534
BaeGI	GKGCMC	3 cut(s)	742, 1141, 1429
BaeI	ACNNNNGTAYC	2 cut(s)	993, 1026
BanI	GGYRCC	2 cut(s)	1136, 1559
BanII	GRGCYC	3 cut(s)	664, 1525, 1764
Bbv12I	GWGCWC	4 cut(s)	742, 1429, 1525, 1832
BbvI	GCAGC	7 cut(s)	23, 640, 662, 2005, 2227, 2306, 2451
BccI	CCATC	7 cut(s)	359, 875, 1013, 1770, 2041, 2180, 2207
BceAI	ACGGC	1 cut(s)	2327
BcgI	CGANNNNNNTGC	4 cut(s)	504, 538, 2213, 2247
BciT130I	CCWGG	5 cut(s)	54, 485, 975, 999, 1812
BciVI	GTATCC	1 cut(s)	2384
BclI	TGATCA	1 cut(s)	2334
BcoDI	GTCTC	4 cut(s)	277, 777, 1199, 1795
BfaI	CTAG	6 cut(s)	92, 351, 381, 1656, 1673, 2237
BfmI	CTRYAG	5 cut(s)	69, 897, 1275, 1581, 1647
BfoI	RGCGCY	1 cut(s)	640
BfrI	CTTAAG	1 cut(s)	1631
BfuI	GTATCC	1 cut(s)	2384
Bme1390I	CCNGG	5 cut(s)	54, 485, 975, 999, 1812
BmeT110I	CYCGRG	1 cut(s)	2353
BmgT120I	GGNCC	1 cut(s)	569
BmiI	GGNNCC	5 cut(s)	1138, 1324, 1352, 1561, 1761
BmrFI	CCNGG	5 cut(s)	54, 485, 975, 999, 1812
BmrI	ACTGGG	4 cut(s)	218, 771, 1077, 2462
BmsI	GCATC	9 cut(s)	145, 179, 229, 853, 946, 1498, 1652, 1729, 1830
BmtI	GCTAGC	2 cut(s)	384, 1659
BmuI	ACTGGG	4 cut(s)	218, 771, 1077, 2462
BpmI	CTGGAG	4 cut(s)	580, 876, 996, 1341
Bpu14I	TTCGAA	1 cut(s)	576
BsaAI	YACGTR	2 cut(s)	1147, 1540
BsaI	GGTCTC	1 cut(s)	777
BsaJI	CCNNGG	5 cut(s)	53, 357, 1570, 2397, 2471
BsaXI	ACNNNNNCTCC	2 cut(s)	1335, 1365
Bsc4I	CCNNNNNNNGG	7 cut(s)	1019, 1361, 1576, 1756, 1817, 1909, 2453
Bse118I	RCCGGY	2 cut(s)	2146, 2480
Bse1I	ACTGG	7 cut(s)	213, 766, 1072, 1825, 2060, 2457, 2574
Bse21I	CCTNAGG	1 cut(s)	2534
Bse3DI	GCAATG	4 cut(s)	391, 645, 1410, 2208
BseBI	CCWGG	5 cut(s)	54, 485, 975, 999, 1812
BseDI	CCNNGG	5 cut(s)	53, 357, 1570, 2397, 2471
BseGI	GGATG	3 cut(s)	694, 1781, 1821
BseLI	CCNNNNNNNGG	7 cut(s)	1019, 1361, 1576, 1756, 1817, 1909, 2453
BseMI	GCAATG	4 cut(s)	391, 645, 1410, 2208
BseMII	CTCAG	2 cut(s)	505, 1806
BseNI	ACTGG	7 cut(s)	213, 766, 1072, 1825, 2060, 2457, 2574
BseRI	GAGGAG	2 cut(s)	1518, 2512
BseSI	GKGCMC	3 cut(s)	742, 1141, 1429
BseXI	GCAGC	7 cut(s)	23, 640, 662, 2005, 2227, 2306, 2451
BsgI	GTGCAG	1 cut(s)	30
Bsh1236I	CGCG	1 cut(s)	701
BshFI	GGCC	3 cut(s)	571, 716, 2402
BshNI	GGYRCC	2 cut(s)	1136, 1559
BsiHKAI	GWGCWC	4 cut(s)	742, 1429, 1525, 1832
BsiHKCI	CYCGRG	1 cut(s)	2353
BsiSI	CCGG	2 cut(s)	2147, 2481
BslFI	GGGAC	2 cut(s)	2049, 2287
BslI	CCNNNNNNNGG	7 cut(s)	1019, 1361, 1576, 1756, 1817, 1909, 2453
BsmAI	GTCTC	4 cut(s)	277, 777, 1199, 1795
BsmFI	GGGAC	2 cut(s)	2049, 2287
BsnI	GGCC	3 cut(s)	571, 716, 2402
Bso31I	GGTCTC	1 cut(s)	777
BsoBI	CYCGRG	1 cut(s)	2353
Bsp119I	TTCGAA	1 cut(s)	576
Bsp1286I	GDGCHC	7 cut(s)	664, 742, 1141, 1429, 1525, 1764, 1832
Bsp1407I	TGTACA	1 cut(s)	178
Bsp143I	GATC	9 cut(s)	109, 331, 353, 1166, 1615, 1767, 2334, 2347, 2457
Bsp19I	CCATGG	2 cut(s)	2397, 2471
BspACI	CCGC	6 cut(s)	453, 1884, 2135, 2430, 2468, 2562
BspANI	GGCC	3 cut(s)	571, 716, 2402
BspCNI	CTCAG	2 cut(s)	504, 1805
BspFNI	CGCG	1 cut(s)	701
BspLI	GGNNCC	5 cut(s)	1138, 1324, 1352, 1561, 1761
BspMAI	CTGCAG	1 cut(s)	1651
BspOI	GCTAGC	2 cut(s)	384, 1659
BspPI	GGATC	6 cut(s)	104, 326, 348, 1174, 1623, 2465
BspT104I	TTCGAA	1 cut(s)	576
BspT107I	GGYRCC	2 cut(s)	1136, 1559
BspTI	CTTAAG	1 cut(s)	1631
BspTNI	GGTCTC	1 cut(s)	777
BsrBI	CCGCTC	1 cut(s)	2135
BsrDI	GCAATG	4 cut(s)	391, 645, 1410, 2208
BsrFI	RCCGGY	2 cut(s)	2146, 2480
BsrGI	TGTACA	1 cut(s)	178
BsrI	ACTGG	7 cut(s)	213, 766, 1072, 1825, 2060, 2457, 2574
BssAI	RCCGGY	2 cut(s)	2146, 2480
BssECI	CCNNGG	5 cut(s)	53, 357, 1570, 2397, 2471
BssMI	GATC	9 cut(s)	109, 331, 353, 1166, 1615, 1767, 2334, 2347, 2457
BssT1I	CCWWGG	4 cut(s)	357, 1570, 2397, 2471
Bst2UI	CCWGG	5 cut(s)	54, 485, 975, 999, 1812
Bst4CI	ACNGT	5 cut(s)	118, 1177, 1276, 1347, 1585
BstAFI	CTTAAG	1 cut(s)	1631
BstAPI	GCANNNNNTGC	1 cut(s)	1827
BstAUI	TGTACA	1 cut(s)	178
BstBAI	YACGTR	2 cut(s)	1147, 1540
BstBI	TTCGAA	1 cut(s)	576
BstDEI	CTNAG	3 cut(s)	491, 1792, 2534
BstDSI	CCRYGG	2 cut(s)	2397, 2471
BstF5I	GGATG	3 cut(s)	694, 1781, 1821
BstFNI	CGCG	1 cut(s)	701
BstH2I	RGCGCY	1 cut(s)	640
BstHHI	GCGC	1 cut(s)	639
BstKTI	GATC	9 cut(s)	112, 334, 356, 1169, 1618, 1770, 2337, 2350, 2460
BstMAI	GTCTC	4 cut(s)	277, 777, 1199, 1795
BstMBI	GATC	9 cut(s)	109, 331, 353, 1166, 1615, 1767, 2334, 2347, 2457
BstNI	CCWGG	5 cut(s)	54, 485, 975, 999, 1812
BstNSI	RCATGY	3 cut(s)	9, 1419, 2490
BstSCI	CCNGG	5 cut(s)	52, 483, 973, 997, 1810
BstSFI	CTRYAG	5 cut(s)	69, 897, 1275, 1581, 1647
BstSLI	GKGCMC	3 cut(s)	742, 1141, 1429
BstUI	CGCG	1 cut(s)	701
BstV1I	GCAGC	7 cut(s)	23, 640, 662, 2005, 2227, 2306, 2451
BstX2I	RGATCY	2 cut(s)	353, 1166
BstXI	CCANNNNNNTGG	2 cut(s)	1347, 2186
BstYI	RGATCY	2 cut(s)	353, 1166
Bsu36I	CCTNAGG	1 cut(s)	2534
BsuI	GTATCC	1 cut(s)	2384
BsuRI	GGCC	3 cut(s)	571, 716, 2402
BtgI	CCRYGG	2 cut(s)	2397, 2471
BtsCI	GGATG	3 cut(s)	694, 1781, 1821
BtsI	GCAGTG	1 cut(s)	1666
BtsIMutI	CAGTG	7 cut(s)	235, 499, 759, 1352, 1666, 1832, 2450
CaiI	CAGNNNCTG	1 cut(s)	1589
CfoI	GCGC	1 cut(s)	639
Cfr10I	RCCGGY	2 cut(s)	2146, 2480
Cfr13I	GGNCC	1 cut(s)	569
Csp6I	GTAC	6 cut(s)	179, 815, 1002, 1148, 1537, 2168
CviQI	GTAC	6 cut(s)	179, 815, 1002, 1148, 1537, 2168
DdeI	CTNAG	3 cut(s)	491, 1792, 2534
DpnI	GATC	9 cut(s)	111, 333, 355, 1168, 1617, 1769, 2336, 2349, 2459
DpnII	GATC	9 cut(s)	109, 331, 353, 1166, 1615, 1767, 2334, 2347, 2457
DrdI	GACNNNNNNGTC	1 cut(s)	291
DseDI	GACNNNNNNGTC	1 cut(s)	291
Ecl136II	GAGCTC	1 cut(s)	1523
Eco130I	CCWWGG	4 cut(s)	357, 1570, 2397, 2471
Eco24I	GRGCYC	3 cut(s)	664, 1525, 1764
Eco31I	GGTCTC	1 cut(s)	777
Eco47III	AGCGCT	1 cut(s)	638
Eco53kI	GAGCTC	1 cut(s)	1523
Eco57I	CTGAAG	1 cut(s)	357
Eco81I	CCTNAGG	1 cut(s)	2534
Eco88I	CYCGRG	1 cut(s)	2353
EcoICRI	GAGCTC	1 cut(s)	1523
EcoRII	CCWGG	5 cut(s)	52, 483, 973, 997, 1810
EcoT14I	CCWWGG	4 cut(s)	357, 1570, 2397, 2471
EcoT22I	ATGCAT	1 cut(s)	2492
EcoT38I	GRGCYC	3 cut(s)	664, 1525, 1764
ErhI	CCWWGG	4 cut(s)	357, 1570, 2397, 2471
FalI	AAGNNNNNCTT	2 cut(s)	415, 447
FaqI	GGGAC	2 cut(s)	2049, 2287
FauNDI	CATATG	1 cut(s)	1963
FbaI	TGATCA	1 cut(s)	2334
FokI	GGATG	3 cut(s)	701, 1788, 1808
FriOI	GRGCYC	3 cut(s)	664, 1525, 1764
FspBI	CTAG	6 cut(s)	92, 351, 381, 1656, 1673, 2237
GlaI	GCGC	1 cut(s)	638
GsuI	CTGGAG	4 cut(s)	580, 876, 996, 1341
HaeII	RGCGCY	1 cut(s)	640
HaeIII	GGCC	3 cut(s)	571, 716, 2402
HapII	CCGG	2 cut(s)	2147, 2481
HhaI	GCGC	1 cut(s)	639
Hin6I	GCGC	1 cut(s)	637
HinP1I	GCGC	1 cut(s)	637
HincII	GTYRAC	2 cut(s)	1099, 1296
HindII	GTYRAC	2 cut(s)	1099, 1296
HinfI	GANTC	3 cut(s)	1887, 2217, 2225
HpaII	CCGG	2 cut(s)	2147, 2481
HphI	GGTGA	5 cut(s)	292, 596, 619, 1120, 2222
Hpy166II	GTNNAC	6 cut(s)	22, 740, 1099, 1296, 1427, 1664
Hpy188I	TCNGA	8 cut(s)	42, 777, 862, 1171, 1236, 1795, 2248, 2378
Hpy8I	GTNNAC	6 cut(s)	22, 740, 1099, 1296, 1427, 1664
HpyAV	CCTTC	6 cut(s)	26, 1151, 1734, 1961, 2374, 2457
HpyCH4III	ACNGT	5 cut(s)	118, 1177, 1276, 1347, 1585
HpyCH4IV	ACGT	5 cut(s)	983, 1103, 1146, 1539, 2170
HpyF3I	CTNAG	3 cut(s)	491, 1792, 2534
HpySE526I	ACGT	5 cut(s)	983, 1103, 1146, 1539, 2170
HspAI	GCGC	1 cut(s)	637
KroI	GCCGGC	1 cut(s)	2146
KroNI	GCCGGC	1 cut(s)	2148
Ksp22I	TGATCA	1 cut(s)	2334
Kzo9I	GATC	9 cut(s)	109, 331, 353, 1166, 1615, 1767, 2334, 2347, 2457
LmnI	GCTCC	7 cut(s)	1231, 1322, 1331, 1356, 1759, 2525, 2535
Lsp1109I	GCAGC	7 cut(s)	23, 640, 662, 2005, 2227, 2306, 2451
LweI	GCATC	9 cut(s)	145, 179, 229, 853, 946, 1498, 1652, 1729, 1830
MaeI	CTAG	6 cut(s)	92, 351, 381, 1656, 1673, 2237
MaeII	ACGT	5 cut(s)	983, 1103, 1146, 1539, 2170
MaeIII	GTNAC	5 cut(s)	343, 1063, 1153, 1585, 2068
MalI	GATC	9 cut(s)	111, 333, 355, 1168, 1617, 1769, 2336, 2349, 2459
MbiI	CCGCTC	1 cut(s)	2135
MboI	GATC	9 cut(s)	109, 331, 353, 1166, 1615, 1767, 2334, 2347, 2457
MboII	GAAGA	4 cut(s)	164, 986, 1943, 2339
MfeI	CAATTG	1 cut(s)	2286
MflI	RGATCY	2 cut(s)	353, 1166
MhlI	GDGCHC	7 cut(s)	664, 742, 1141, 1429, 1525, 1764, 1832
MlyI	GAGTC	1 cut(s)	2219
MmeI	TCCRAC	5 cut(s)	800, 919, 1144, 1837, 1922
Mph1103I	ATGCAT	1 cut(s)	2492
MroNI	GCCGGC	1 cut(s)	2146
MroXI	GAANNNNTTC	2 cut(s)	36, 673
MseI	TTAA	6 cut(s)	915, 986, 1194, 1533, 1632, 2121
MslI	CAYNNNNRTG	4 cut(s)	515, 1106, 1610, 2495
MspA1I	CMGCKG	3 cut(s)	653, 1451, 2297
MspCI	CTTAAG	1 cut(s)	1631
MspI	CCGG	2 cut(s)	2147, 2481
MspR9I	CCNGG	5 cut(s)	54, 485, 975, 999, 1812
MunI	CAATTG	1 cut(s)	2286
MvaI	CCWGG	5 cut(s)	54, 485, 975, 999, 1812
MvnI	CGCG	1 cut(s)	701
NaeI	GCCGGC	1 cut(s)	2148
NcoI	CCATGG	2 cut(s)	2397, 2471
NdeI	CATATG	1 cut(s)	1963
NdeII	GATC	9 cut(s)	109, 331, 353, 1166, 1615, 1767, 2334, 2347, 2457
NgoMIV	GCCGGC	1 cut(s)	2146
NheI	GCTAGC	2 cut(s)	380, 1655
NlaIV	GGNNCC	5 cut(s)	1138, 1324, 1352, 1561, 1761
NmuCI	GTSAC	1 cut(s)	343
NsiI	ATGCAT	1 cut(s)	2492
NspI	RCATGY	3 cut(s)	9, 1419, 2490
NspV	TTCGAA	1 cut(s)	576
OliI	CACNNNNGTG	1 cut(s)	1106
PaeI	GCATGC	2 cut(s)	1419, 2490
PaeR7I	CTCGAG	1 cut(s)	2353
PciI	ACATGT	1 cut(s)	5
PdiI	GCCGGC	1 cut(s)	2148
PdmI	GAANNNNTTC	2 cut(s)	36, 673
PfeI	GAWTC	2 cut(s)	1887, 2217
PflFI	GACNNNGTC	1 cut(s)	137
PleI	GAGTC	1 cut(s)	2219
PpsI	GAGTC	1 cut(s)	2219
Ppu21I	YACGTR	2 cut(s)	1147, 1540
PscI	ACATGT	1 cut(s)	5
PshBI	ATTAAT	1 cut(s)	1194
Psp124BI	GAGCTC	1 cut(s)	1525
Psp6I	CCWGG	5 cut(s)	52, 483, 973, 997, 1810
PspGI	CCWGG	5 cut(s)	52, 483, 973, 997, 1810
PspN4I	GGNNCC	5 cut(s)	1138, 1324, 1352, 1561, 1761
PspPI	GGNCC	1 cut(s)	569
PstI	CTGCAG	1 cut(s)	1651
PstNI	CAGNNNCTG	1 cut(s)	1589
PsuI	RGATCY	2 cut(s)	353, 1166
PsyI	GACNNNGTC	1 cut(s)	137
PvuII	CAGCTG	3 cut(s)	653, 1451, 2297
RsaI	GTAC	6 cut(s)	180, 816, 1003, 1149, 1538, 2169
RsaNI	GTAC	6 cut(s)	179, 815, 1002, 1148, 1537, 2168
RseI	CAYNNNNRTG	4 cut(s)	515, 1106, 1610, 2495
SacI	GAGCTC	1 cut(s)	1525
SaqAI	TTAA	6 cut(s)	915, 986, 1194, 1533, 1632, 2121
Sau3AI	GATC	9 cut(s)	109, 331, 353, 1166, 1615, 1767, 2334, 2347, 2457
Sau96I	GGNCC	1 cut(s)	569
SchI	GAGTC	1 cut(s)	2219
ScrFI	CCNGG	5 cut(s)	54, 485, 975, 999, 1812
SduI	GDGCHC	7 cut(s)	664, 742, 1141, 1429, 1525, 1764, 1832
SfaNI	GCATC	9 cut(s)	145, 179, 229, 853, 946, 1498, 1652, 1729, 1830
SfcI	CTRYAG	5 cut(s)	69, 897, 1275, 1581, 1647
Sfr274I	CTCGAG	1 cut(s)	2353
SfuI	TTCGAA	1 cut(s)	576
SlaI	CTCGAG	1 cut(s)	2353
SmiMI	CAYNNNNRTG	4 cut(s)	515, 1106, 1610, 2495
SmlI	CTYRAG	2 cut(s)	1631, 2353
SmoI	CTYRAG	2 cut(s)	1631, 2353
SphI	GCATGC	2 cut(s)	1419, 2490
SsiI	CCGC	6 cut(s)	453, 1884, 2135, 2430, 2468, 2562
SspMI	CTAG	6 cut(s)	92, 351, 381, 1656, 1673, 2237
SstI	GAGCTC	1 cut(s)	1525
StyD4I	CCNGG	5 cut(s)	52, 483, 973, 997, 1810
StyI	CCWWGG	4 cut(s)	357, 1570, 2397, 2471
TaaI	ACNGT	5 cut(s)	118, 1177, 1276, 1347, 1585
TaiI	ACGT	5 cut(s)	986, 1106, 1149, 1542, 2173
TaqI	TCGA	5 cut(s)	514, 576, 1203, 1890, 2354
TatI	WGTACW	2 cut(s)	178, 814
TauI	GCSGC	3 cut(s)	455, 2432, 2470
TfiI	GAWTC	2 cut(s)	1887, 2217
Tru1I	TTAA	6 cut(s)	915, 986, 1194, 1533, 1632, 2121
Tru9I	TTAA	6 cut(s)	915, 986, 1194, 1533, 1632, 2121
TscAI	CASTG	7 cut(s)	235, 499, 766, 1352, 1666, 1832, 2457
TseFI	GTSAC	1 cut(s)	343
TseI	GCWGC	7 cut(s)	11, 650, 653, 2018, 2240, 2294, 2464
Tsp45I	GTSAC	1 cut(s)	343
TspDTI	ATGAA	5 cut(s)	462, 463, 537, 1980, 2163
TspRI	CASTG	7 cut(s)	235, 499, 766, 1352, 1666, 1832, 2457
Tth111I	GACNNNGTC	1 cut(s)	137
Vha464I	CTTAAG	1 cut(s)	1631
VneI	GTGCAC	2 cut(s)	738, 1425
VspI	ATTAAT	1 cut(s)	1194
XapI	RAATTY	3 cut(s)	1126, 1238, 1599
XbaI	TCTAGA	1 cut(s)	350
XceI	RCATGY	3 cut(s)	9, 1419, 2490
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1402
XhoI	CTCGAG	1 cut(s)	2353
XmnI	GAANNNNTTC	2 cut(s)	36, 673
XspI	CTAG	6 cut(s)	92, 351, 381, 1656, 1673, 2237
Zsp2I	ATGCAT	1 cut(s)	2492

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.