Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0009249

Physical Location & Seq

Genomic Coordinates Reverse (-)

32553983 .. 32560151

Gene Structure 6169 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1228800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3009 bp

ATGGCGCCGAGTCCCAGATACCTCCAGCTTACCCAAGTCTCCGCCGGAGATCGGAAGTCGATGGTTGCCGCCGGCGACTTCGGTGATTCGGAGGACGTGAGGCTCCTGGATTCCTATGAGAATTCGGAGGGAGTTGAAGCGGGGATGAAGAGGGTTCAAGTCAGAGTCTCTGGTATGACATGTGCTGCTTGCTCGAATTCTGTCGAAGGTGCTCTGCAGAGCGTGAATGGGGTTCTCACGGCCTCCGTGGCGTTGCTCCAGAACAGGGCCGATGTGGTTTTCGATCCGAGATTGATCAAGGATGAAGACATCAAGAAAGCAATTGAAGATGCTGGGTTTGACGCAGAGGTTATACTTGAGCCCAGTGCAAGCGGAACAAAGCCACATGGTACCCTGTTGGGCCAGTTCTCAATTGGAGGTATGACCTGTGCAGCCTGTGTGAACTCTGTTGAAGGTATTCTAAAAGGGCTTCCTGGAGTCAAAAGAGCCGTAGTAGCTCTGGCTACTTCACTGGGGGAAGTGGAGTATGATCCTACATTAATTAGTAAAGATGATATAGTGAATGCCATTGAAGATGCTGGTTTTGATGCATCACTTGTACAGAGTAGCGAACAGGATAAAATCATACTGGGAGTTGCTGGCGTATTCAGTGAGATGGATGCACAGATGTTAGAAGCCATAATTATCAACTTGAAAGGTGTGAGACATTTTCGTTTTGACAGGATTTCAAGCGAACTTGAAATTCTCTTTGATCCTGAAGTTGTAACTTCTAGATCCTTGGTTGATGGGATTAATGAGGCCAGCAATGAGAAATTTAAACTACAAGTGGCAAACCCTTATACAAGAATGACTTCTAAAGACATCGGTGAAGCTTCAAATATTTTTCGGCTATTTCTTTCCAGTCTGCTTCTCAGTATTCCTATCTTTTTCATTCGAGTAGTTTGTCCCCATATTCCGCTTCTGTATTCATTACTGCTCCGGAGATGTGGGCCCTTCGTAATGGGTGATTGGTTGAAATGGGCGCTGGTCAGTGTTGTTCAGTTTGTTATTGGGAAGCGCTTCTATGTTGCAGCTGCCAGAGCTCTACGAAATGGTTCAACCAACATGGATGTTTTGGTTGCATTGGGAACTTCGGCCTCTTACTTCTATTCTGTATGCGCGCTTCTATATGGTGCAGTCACTGGGTTCTGGTCTCAAACTTACTTTGAAACAAGTGCCATGCTAATAACATTTGTACTTTTGGGGAAGTATCTGGAGTGTCTTGCAAAAGGAAAAACTTCAGATGCCATCAAGAAATTGATAGAACTTGCTCCAGCTACAGCATTGTTGCTTGTCAAAGACAAAGGTGGGAAAGTCATTGGAGAAAGGGAAATTGATGCCTTGCTAATTCAGCCCGGGGATGTGTTAAAAGTTCTTCCTGGTACAAAGGTTCCTGCTGATGGTATGGTTGTGTGGGGTTCAAGCTATGTCAATGAGAGTATGGTGACTGGTGAAGCTATACCTGTTTCAAAGGAAGTAAATTCATTAGTTATTGGAGGAACAATAAATTTACATGGCGCCCTTCACATACAAATTACAAAAGTTGGATCAGATACAGTTTTAAGTCAGATAATTAATTTGGTTGAGACTGCTCAGATGTCCAAAGCTCCTATTCAGAAATTTGCTGATTTTGTTGCTAGCATTTTTGTCCCTACTGTAGTTGCCTTGGCATTGTTGACATTATTGGGTTGGTATACTGCTGGAGCCTTTGGAGCTTACCCAGAAGAGTGGCTCCCTGAAAACGGAAATCACTTTGTATTTGCCCTTATGTTTTCCATATCAGTTGTGGTGATTGCATGTCCTTGTGCACTGGGCTTGGCAACACCAACTGCTGTCATGGTTGCGACGGGGGTTGGCGCTAATAATGGTGTGCTGATAAAAGGAGGAGATGCTTTGGAAAGAGCTCAGAAGATCAAGTATGTGATATTTGATAAAACAGGTACCCTGACCCAGGGAAAAGCTACGGTTACTACTGTAAAAGTTTTCACGGGAATGGATCGTGGAGAATTTCTTAAATTGGTGGCTTCTGCAGAGGCTAGCAGTGAACATCCACTGGCAAAAGCAATAGTGGAATATGCACGCCATTTTCACTTTTTTGATGAGCCTTCTGTAGCTGACGATGCACCGAACAAGAGCAAAGACACCACAATTTCTGGATGGCTTTTTGATGCCTCAGAGTTCTCTGCTTTGCCCGGAAGAGGTATCCAGTGCTTTATAGATGGAAAACTTATTCTGGTCGGTAATAGGAAGCTGATGACTGAAAGTGGAATCAACATTCCTACACATGTAGAGAATTTTGTTGTAGAGCTGGAAGAAAGTGCAAAGACGGGCATAATTGTTGCATACGAAGGCAATTTACTTGGTGTTTTGGGGGTTGCGGACCCTCTAAAAAGAGAAGCTGCCATAGTAATCGAGGGACTTCGTAAGATGGGTGTCATACCAGTCATGGTTACTGGGGATAATCGTCGGACAGCACAGGCTGTAGCTAAGGAGGTTGGCATTCAAGACGTGAGGGCAGAGGTAATGCCGGCTGGAAAAGCTGATGCTGTATGTTCATTCCAAAAAGATGGAAGTATAGTTGCTATGGTTGGTGATGGAATCAATGACTCACCTGCCCTAGCTGCTTCTGATGTTGGTATGGCGATCGGTGCAGGGACCGATATTGCAATTGAAGCAGCTGACTACGTGTTGATGAGAAATAACTTGGAAGACGTAATCACAGCCATTGATCTCTCAAGGAAGACCTTCACTCGTATTCGATTGAACTACGTGTTTGCCATGGCGTACAATGTGATCGCAATACCTATTGCTGCCGGGGTCTTCTTCCCATCACTGGGGATCATGTTGCCGCCGTGGGCGGCCGGTGCATGCATGGCCATGTCATCTGTTAGTGTTGTATGCTCTTCTTTGCTCCTTAGGAGATACAGAAAACCCAGACTTACGGCCATACTGGAAATAGTTGTAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0012505 GO:0016020 GO:0023052 GO:0031410 GO:0031982 GO:0031984 GO:0032870 GO:0035556 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071369 GO:0071495 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1003

Amino Acids

107.08

Weight (kDa)

5.3

Isoelectric Point (pI)

31.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	54 - 114	9.1e-15	Heavy-metal-associated domain
HMA	PF00403	136 - 196	5.8e-15	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	438 - 542	1.1e-23	P-type ATPase actuator domain
Hydrolase	PF00702	651 - 889	1.3e-30	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44790
fragaria_vesca	FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710
malus_domestica	MD05G1247900.v1.1 MD10G1228800.v1.1
prunus_persica	Prupe.4G115900_v2.0.a1 Prupe.4G115900_v2.0.a1
pyrus_communis	pycom05g22420 pycom10g19240
rosa_chinensis	RchiOBHm_Chr5g0020701
rosa_laevigata	RLG00000032554
rosa_multiflora	Rmu_sc0005607.1_g000032
rosa_roxburghii	Rroxscaffold_1G00057600
rosa_rugosa	Rorug05G0062500
rosa_samantha	Rh5AG152200 Rh5BG151900 Rh5CG163300 Rh5DG151400
rosa_wichuraiana	Rw5G013490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2659
Acc36I	ACCTGC	1 cut(s)	2659
Acc65I	GGTACC	2 cut(s)	389, 1979
AccB1I	GGYRCC	4 cut(s)	4, 389, 1556, 1979
AccI	GTMKAC	1 cut(s)	1733
AccII	CGCG	1 cut(s)	1160
AccIII	TCCGGA	1 cut(s)	978
AciI	CCGC	8 cut(s)	42, 69, 140, 372, 956, 2417, 2888, 2897
AclWI	GGATC	7 cut(s)	278, 524, 746, 768, 1594, 2043, 2885
AcoI	YGGCCR	3 cut(s)	2898, 2913, 2982
AcsI	RAATTY	9 cut(s)	121, 196, 741, 812, 1519, 1546, 1658, 2045, 2332
AcuI	CTGAAG	2 cut(s)	777, 1263
AcyI	GRCGYC	2 cut(s)	5, 1557
AfaI	GTAC	6 cut(s)	391, 600, 1236, 1423, 1981, 2825
AfeI	AGCGCT	1 cut(s)	1058
AfiI	CCNNNNNNNGG	8 cut(s)	51, 265, 1439, 1781, 1990, 2237, 2872, 2873
AflIII	ACRYGT	4 cut(s)	179, 2323, 2724, 2808
AjiI	CACGTC	2 cut(s)	97, 2548
AjnI	CCWGG	4 cut(s)	105, 472, 1417, 1989
Alw21I	GWGCWC	4 cut(s)	214, 1084, 1849, 1945
Alw26I	GTCTC	5 cut(s)	43, 172, 697, 1197, 1619
Alw44I	GTGCAC	1 cut(s)	1845
AlwI	GGATC	7 cut(s)	278, 524, 746, 768, 1594, 2043, 2885
AlwNI	CAGNNNCTG	1 cut(s)	1181
Ama87I	CYCGRG	1 cut(s)	1394
Aor13HI	TCCGGA	1 cut(s)	978
Aor51HI	AGCGCT	1 cut(s)	1058
AoxI	GGCC	9 cut(s)	240, 267, 400, 798, 989, 1134, 2898, 2913, 2982
ApaI	GGGCCC	1 cut(s)	993
ApaLI	GTGCAC	1 cut(s)	1845
ApeKI	GCWGC	8 cut(s)	185, 431, 1070, 1073, 2438, 2660, 2714, 2849
ApoI	RAATTY	9 cut(s)	121, 196, 741, 812, 1519, 1546, 1658, 2045, 2332
AseI	ATTAAT	3 cut(s)	539, 792, 1614
Asp700I	GAANNNNTTC	6 cut(s)	456, 850, 1058, 1093, 1276, 2783
Asp718I	GGTACC	2 cut(s)	389, 1979
AspLEI	GCGC	7 cut(s)	7, 1024, 1059, 1160, 1162, 1559, 1898
AspS9I	GGNCC	6 cut(s)	267, 400, 989, 990, 2419, 2694
AsuC2I	CCSGG	4 cut(s)	1395, 1396, 2232, 2854
AsuHPI	GGTGA	8 cut(s)	95, 878, 1016, 1495, 1502, 1840, 2640, 2642
AsuNHI	GCTAGC	2 cut(s)	1676, 2075
AvaI	CYCGRG	1 cut(s)	1394
AvaII	GGWCC	2 cut(s)	2419, 2694
AxyI	CCTNAGG	1 cut(s)	2954
BaeGI	GKGCMC	2 cut(s)	993, 1849
BaeI	ACNNNNGTAYC	2 cut(s)	1413, 1446
BalI	TGGCCA	1 cut(s)	2915
BanI	GGYRCC	4 cut(s)	4, 389, 1556, 1979
BanII	GRGCYC	4 cut(s)	363, 993, 1084, 1945
BarI	GAAGNNNNNNTAC	4 cut(s)	1146, 1178, 2379, 2411
BbsI	GAAGAC	4 cut(s)	312, 2754, 2786, 2851
Bbv12I	GWGCWC	4 cut(s)	214, 1084, 1849, 1945
BbvI	GCAGC	8 cut(s)	172, 443, 1060, 1082, 2425, 2647, 2726, 2836
BceAI	ACGGC	4 cut(s)	255, 473, 2875, 2997
BciT130I	CCWGG	4 cut(s)	107, 474, 1419, 1991
BciVI	GTATCC	1 cut(s)	2252
BclI	TGATCA	1 cut(s)	294
BcnI	CCSGG	4 cut(s)	1395, 1396, 2232, 2854
BcoDI	GTCTC	5 cut(s)	43, 172, 697, 1197, 1619
BfaI	CTAG	4 cut(s)	771, 1677, 2076, 2657
BfmI	CTRYAG	6 cut(s)	215, 1317, 1695, 2067, 2148, 2520
BfoI	RGCGCY	5 cut(s)	8, 1025, 1060, 1560, 1899
BfuAI	ACCTGC	1 cut(s)	2659
BfuI	GTATCC	1 cut(s)	2252
BglI	GCCNNNNNGGC	1 cut(s)	248
Bme1390I	CCNGG	8 cut(s)	107, 474, 1395, 1396, 1419, 1991, 2232, 2854
Bme18I	GGWCC	2 cut(s)	2419, 2694
BmeT110I	CYCGRG	1 cut(s)	1394
BmgBI	CACGTC	2 cut(s)	97, 2548
BmgT120I	GGNCC	6 cut(s)	267, 400, 989, 990, 2419, 2694
BmrFI	CCNGG	8 cut(s)	107, 474, 1395, 1396, 1419, 1991, 2232, 2854
BmrI	ACTGGG	7 cut(s)	357, 521, 638, 1191, 1859, 2502, 2882
BmtI	GCTAGC	2 cut(s)	1680, 2079
BmuI	ACTGGG	7 cut(s)	357, 521, 638, 1191, 1859, 2502, 2882
BpiI	GAAGAC	4 cut(s)	312, 2754, 2786, 2851
BpmI	CTGGAG	6 cut(s)	8, 242, 495, 1274, 1296, 1761
Bpu10I	CCTNAGC	1 cut(s)	2526
BpuEI	CTTGAG	2 cut(s)	377, 2758
BpuMI	CCSGG	4 cut(s)	1395, 1396, 2232, 2854
BsaAI	YACGTR	2 cut(s)	2725, 2809
BsaHI	GRCGYC	2 cut(s)	5, 1557
BsaI	GGTCTC	1 cut(s)	1197
BsaWI	WCCGGW	1 cut(s)	978
Bsc4I	CCNNNNNNNGG	8 cut(s)	51, 265, 1439, 1781, 1990, 2237, 2872, 2873
Bse118I	RCCGGY	3 cut(s)	71, 2566, 2900
Bse21I	CCTNAGG	1 cut(s)	2954
Bse3DI	GCAATG	1 cut(s)	811
BseAI	TCCGGA	1 cut(s)	978
BseBI	CCWGG	4 cut(s)	107, 474, 1419, 1991
BseGI	GGATG	7 cut(s)	150, 307, 664, 1114, 1405, 2086, 2201
BseLI	CCNNNNNNNGG	8 cut(s)	51, 265, 1439, 1781, 1990, 2237, 2872, 2873
BseMI	GCAATG	1 cut(s)	811
BseMII	CTCAG	4 cut(s)	925, 1646, 1958, 2226
BsePI	GCGCGC	1 cut(s)	1158
BseRI	GAGGAG	1 cut(s)	1938
BseSI	GKGCMC	2 cut(s)	993, 1849
BseX3I	CGGCCG	1 cut(s)	2898
BseXI	GCAGC	8 cut(s)	172, 443, 1060, 1082, 2425, 2647, 2726, 2836
BseYI	CCCAGC	1 cut(s)	332
BsgI	GTGCAG	3 cut(s)	450, 1194, 2709
Bsh1236I	CGCG	1 cut(s)	1160
Bsh1285I	CGRYCG	2 cut(s)	2685, 2901
BshFI	GGCC	9 cut(s)	242, 269, 402, 800, 991, 1136, 2900, 2915, 2984
BshNI	GGYRCC	4 cut(s)	4, 389, 1556, 1979
BsiEI	CGRYCG	2 cut(s)	2685, 2901
BsiHKAI	GWGCWC	4 cut(s)	214, 1084, 1849, 1945
BsiHKCI	CYCGRG	1 cut(s)	1394
BsiSI	CCGG	8 cut(s)	45, 72, 979, 1395, 2232, 2567, 2853, 2901
BslFI	GGGAC	4 cut(s)	930, 1673, 2469, 2707
BslI	CCNNNNNNNGG	8 cut(s)	51, 265, 1439, 1781, 1990, 2237, 2872, 2873
BsmAI	GTCTC	5 cut(s)	43, 172, 697, 1197, 1619
BsmFI	GGGAC	4 cut(s)	930, 1673, 2469, 2707
BsmI	GAATGC	2 cut(s)	568, 2538
BsnI	GGCC	9 cut(s)	242, 269, 402, 800, 991, 1136, 2900, 2915, 2984
Bso31I	GGTCTC	1 cut(s)	1197
BsoBI	CYCGRG	1 cut(s)	1394
Bsp120I	GGGCCC	1 cut(s)	989
Bsp1286I	GDGCHC	6 cut(s)	214, 363, 993, 1084, 1849, 1945
Bsp13I	TCCGGA	1 cut(s)	978
Bsp1407I	TGTACA	1 cut(s)	598
Bsp19I	CCATGG	1 cut(s)	2817
BspACI	CCGC	8 cut(s)	42, 69, 140, 372, 956, 2417, 2888, 2897
BspANI	GGCC	9 cut(s)	242, 269, 402, 800, 991, 1136, 2900, 2915, 2984
BspCNI	CTCAG	4 cut(s)	924, 1645, 1957, 2225
BspEI	TCCGGA	1 cut(s)	978
BspFNI	CGCG	1 cut(s)	1160
BspMAI	CTGCAG	2 cut(s)	219, 2071
BspMI	ACCTGC	1 cut(s)	2659
BspOI	GCTAGC	2 cut(s)	1680, 2079
BspPI	GGATC	7 cut(s)	278, 524, 746, 768, 1594, 2043, 2885
BspQI	GCTCTTC	1 cut(s)	2947
BspT107I	GGYRCC	4 cut(s)	4, 389, 1556, 1979
BspTNI	GGTCTC	1 cut(s)	1197
BsrDI	GCAATG	1 cut(s)	811
BsrFI	RCCGGY	3 cut(s)	71, 2566, 2900
BsrGI	TGTACA	1 cut(s)	598
BssAI	RCCGGY	3 cut(s)	71, 2566, 2900
BssHII	GCGCGC	1 cut(s)	1158
BssNAI	GTATAC	1 cut(s)	1734
BssNI	GRCGYC	2 cut(s)	5, 1557
BssT1I	CCWWGG	3 cut(s)	777, 1704, 2817
Bst1107I	GTATAC	1 cut(s)	1734
Bst2UI	CCWGG	4 cut(s)	107, 474, 1419, 1991
Bst4CI	ACNGT	4 cut(s)	1597, 1696, 2005, 2014
Bst6I	CTCTTC	4 cut(s)	143, 1758, 2230, 2947
BstACI	GRCGYC	2 cut(s)	5, 1557
BstAUI	TGTACA	1 cut(s)	598
BstBAI	YACGTR	2 cut(s)	2725, 2809
BstDEI	CTNAG	6 cut(s)	911, 1632, 1944, 2212, 2526, 2954
BstDSI	CCRYGG	3 cut(s)	246, 2817, 2891
BstENI	CCTNNNNNAGG	1 cut(s)	1988
BstF5I	GGATG	7 cut(s)	150, 307, 664, 1114, 1405, 2086, 2201
BstFNI	CGCG	1 cut(s)	1160
BstH2I	RGCGCY	5 cut(s)	8, 1025, 1060, 1560, 1899
BstHHI	GCGC	7 cut(s)	7, 1024, 1059, 1160, 1162, 1559, 1898
BstMAI	GTCTC	5 cut(s)	43, 172, 697, 1197, 1619
BstMCI	CGRYCG	2 cut(s)	2685, 2901
BstNI	CCWGG	4 cut(s)	107, 474, 1419, 1991
BstNSI	RCATGY	4 cut(s)	183, 1839, 2327, 2910
BstSCI	CCNGG	8 cut(s)	105, 472, 1393, 1394, 1417, 1989, 2230, 2852
BstSFI	CTRYAG	6 cut(s)	215, 1317, 1695, 2067, 2148, 2520
BstSLI	GKGCMC	2 cut(s)	993, 1849
BstUI	CGCG	1 cut(s)	1160
BstV1I	GCAGC	8 cut(s)	172, 443, 1060, 1082, 2425, 2647, 2726, 2836
BstV2I	GAAGAC	4 cut(s)	312, 2754, 2786, 2851
BstX2I	RGATCY	1 cut(s)	773
BstXI	CCANNNNNNTGG	2 cut(s)	1767, 2606
BstYI	RGATCY	1 cut(s)	773
BstZ17I	GTATAC	1 cut(s)	1734
BstZI	CGGCCG	1 cut(s)	2898
Bsu36I	CCTNAGG	1 cut(s)	2954
BsuI	GTATCC	1 cut(s)	2252
BsuRI	GGCC	9 cut(s)	242, 269, 402, 800, 991, 1136, 2900, 2915, 2984
BtgI	CCRYGG	3 cut(s)	246, 2817, 2891
BtrI	CACGTC	2 cut(s)	97, 2548
BtsCI	GGATG	7 cut(s)	150, 307, 664, 1114, 1405, 2086, 2201
BtsI	GCAGTG	1 cut(s)	2086
BveI	ACCTGC	1 cut(s)	2659
CaiI	CAGNNNCTG	1 cut(s)	1181
CfoI	GCGC	7 cut(s)	7, 1024, 1059, 1160, 1162, 1559, 1898
Cfr10I	RCCGGY	3 cut(s)	71, 2566, 2900
Cfr13I	GGNCC	6 cut(s)	267, 400, 989, 990, 2419, 2694
Cfr9I	CCCGGG	1 cut(s)	1394
CseI	GACGC	1 cut(s)	350
Csp6I	GTAC	6 cut(s)	390, 599, 1235, 1422, 1980, 2824
CviQI	GTAC	6 cut(s)	390, 599, 1235, 1422, 1980, 2824
DdeI	CTNAG	6 cut(s)	911, 1632, 1944, 2212, 2526, 2954
DinI	GGCGCC	2 cut(s)	6, 1558
DraI	TTTAAA	1 cut(s)	817
EaeI	YGGCCR	3 cut(s)	2898, 2913, 2982
EagI	CGGCCG	1 cut(s)	2898
Eam1104I	CTCTTC	4 cut(s)	143, 1758, 2230, 2947
EarI	CTCTTC	4 cut(s)	143, 1758, 2230, 2947
EciI	GGCGGA	1 cut(s)	31
Ecl136II	GAGCTC	2 cut(s)	1082, 1943
EclXI	CGGCCG	1 cut(s)	2898
Eco130I	CCWWGG	3 cut(s)	777, 1704, 2817
Eco24I	GRGCYC	4 cut(s)	363, 993, 1084, 1945
Eco31I	GGTCTC	1 cut(s)	1197
Eco47I	GGWCC	2 cut(s)	2419, 2694
Eco47III	AGCGCT	1 cut(s)	1058
Eco52I	CGGCCG	1 cut(s)	2898
Eco53kI	GAGCTC	2 cut(s)	1082, 1943
Eco57I	CTGAAG	2 cut(s)	777, 1263
Eco81I	CCTNAGG	1 cut(s)	2954
Eco88I	CYCGRG	1 cut(s)	1394
EcoICRI	GAGCTC	2 cut(s)	1082, 1943
EcoNI	CCTNNNNNAGG	1 cut(s)	1988
EcoO109I	RGGNCCY	1 cut(s)	990
EcoRI	GAATTC	2 cut(s)	121, 196
EcoRII	CCWGG	4 cut(s)	105, 472, 1417, 1989
EcoT14I	CCWWGG	3 cut(s)	777, 1704, 2817
EcoT22I	ATGCAT	2 cut(s)	592, 2912
EcoT38I	GRGCYC	4 cut(s)	363, 993, 1084, 1945
EgeI	GGCGCC	2 cut(s)	6, 1558
EheI	GGCGCC	2 cut(s)	6, 1558
ErhI	CCWWGG	3 cut(s)	777, 1704, 2817
FalI	AAGNNNNNCTT	2 cut(s)	835, 867
FaqI	GGGAC	4 cut(s)	930, 1673, 2469, 2707
FauI	CCCGC	1 cut(s)	133
FbaI	TGATCA	1 cut(s)	294
FblI	GTMKAC	1 cut(s)	1733
FokI	GGATG	7 cut(s)	157, 314, 671, 1121, 1412, 2073, 2208
FriOI	GRGCYC	4 cut(s)	363, 993, 1084, 1945
FspBI	CTAG	4 cut(s)	771, 1677, 2076, 2657
GlaI	GCGC	7 cut(s)	6, 1023, 1058, 1159, 1161, 1558, 1897
GsaI	CCCAGC	1 cut(s)	336
GsuI	CTGGAG	6 cut(s)	8, 242, 495, 1274, 1296, 1761
HaeII	RGCGCY	5 cut(s)	8, 1025, 1060, 1560, 1899
HaeIII	GGCC	9 cut(s)	242, 269, 402, 800, 991, 1136, 2900, 2915, 2984
HapII	CCGG	8 cut(s)	45, 72, 979, 1395, 2232, 2567, 2853, 2901
HgaI	GACGC	1 cut(s)	350
HhaI	GCGC	7 cut(s)	7, 1024, 1059, 1160, 1162, 1559, 1898
Hin1I	GRCGYC	2 cut(s)	5, 1557
Hin6I	GCGC	7 cut(s)	5, 1022, 1057, 1158, 1160, 1557, 1896
HinP1I	GCGC	7 cut(s)	5, 1022, 1057, 1158, 1160, 1557, 1896
HincII	GTYRAC	1 cut(s)	1716
HindII	GTYRAC	1 cut(s)	1716
HindIII	AAGCTT	1 cut(s)	870
HinfI	GANTC	8 cut(s)	10, 86, 110, 165, 477, 2307, 2637, 2645
HpaII	CCGG	8 cut(s)	45, 72, 979, 1395, 2232, 2567, 2853, 2901
HphI	GGTGA	8 cut(s)	95, 878, 1016, 1495, 1502, 1840, 2640, 2642
Hpy166II	GTNNAC	5 cut(s)	442, 1716, 1734, 1847, 2084
Hpy188III	TCNNGA	9 cut(s)	259, 313, 755, 771, 979, 1253, 1291, 2193, 2543
Hpy8I	GTNNAC	5 cut(s)	442, 1716, 1734, 1847, 2084
Hpy99I	CGWCG	2 cut(s)	1888, 2508
HpyAV	CCTTC	7 cut(s)	200, 446, 1003, 1571, 2154, 2381, 2794
HpyCH4III	ACNGT	4 cut(s)	1597, 1696, 2005, 2014
HpyCH4IV	ACGT	5 cut(s)	96, 2547, 2724, 2751, 2808
HpyF3I	CTNAG	6 cut(s)	911, 1632, 1944, 2212, 2526, 2954
HpySE526I	ACGT	5 cut(s)	96, 2547, 2724, 2751, 2808
Hsp92I	GRCGYC	2 cut(s)	5, 1557
HspAI	GCGC	7 cut(s)	5, 1022, 1057, 1158, 1160, 1557, 1896
KasI	GGCGCC	2 cut(s)	4, 1556
Kpn2I	TCCGGA	1 cut(s)	978
KpnI	GGTACC	2 cut(s)	393, 1983
KroI	GCCGGC	2 cut(s)	71, 2566
KroNI	GCCGGC	2 cut(s)	73, 2568
Ksp22I	TGATCA	1 cut(s)	294
LguI	GCTCTTC	1 cut(s)	2947
LmnI	GCTCC	9 cut(s)	108, 261, 981, 1315, 1651, 1742, 1751, 1776, 2955
Lsp1109I	GCAGC	8 cut(s)	172, 443, 1060, 1082, 2425, 2647, 2726, 2836
MaeI	CTAG	4 cut(s)	771, 1677, 2076, 2657
MaeII	ACGT	5 cut(s)	96, 2547, 2724, 2751, 2808
MaeIII	GTNAC	5 cut(s)	763, 1177, 1483, 2005, 2488
MfeI	CAATTG	3 cut(s)	321, 411, 2706
MflI	RGATCY	1 cut(s)	773
MhlI	GDGCHC	6 cut(s)	214, 363, 993, 1084, 1849, 1945
MlsI	TGGCCA	1 cut(s)	2915
MluNI	TGGCCA	1 cut(s)	2915
Mly113I	GGCGCC	2 cut(s)	5, 1557
MlyI	GAGTC	4 cut(s)	19, 174, 486, 2639
MmeI	TCCRAC	2 cut(s)	1564, 2486
Mox20I	TGGCCA	1 cut(s)	2915
Mph1103I	ATGCAT	2 cut(s)	592, 2912
MreI	CGCCGGCG	1 cut(s)	71
MroI	TCCGGA	1 cut(s)	978
MroNI	GCCGGC	2 cut(s)	71, 2566
MroXI	GAANNNNTTC	6 cut(s)	456, 850, 1058, 1093, 1276, 2783
MscI	TGGCCA	1 cut(s)	2915
MseI	TTAA	7 cut(s)	539, 792, 816, 1406, 1601, 1614, 2052
MslI	CAYNNNNRTG	2 cut(s)	2030, 2915
Msp20I	TGGCCA	1 cut(s)	2915
MspA1I	CMGCKG	2 cut(s)	1073, 2717
MspI	CCGG	8 cut(s)	45, 72, 979, 1395, 2232, 2567, 2853, 2901
MspR9I	CCNGG	8 cut(s)	107, 474, 1395, 1396, 1419, 1991, 2232, 2854
MunI	CAATTG	3 cut(s)	321, 411, 2706
Mva1269I	GAATGC	2 cut(s)	568, 2538
MvaI	CCWGG	4 cut(s)	107, 474, 1419, 1991
MvnI	CGCG	1 cut(s)	1160
NaeI	GCCGGC	2 cut(s)	73, 2568
NarI	GGCGCC	2 cut(s)	5, 1557
NciI	CCSGG	4 cut(s)	1395, 1396, 2232, 2854
NcoI	CCATGG	1 cut(s)	2817
NgoMIV	GCCGGC	2 cut(s)	71, 2566
NheI	GCTAGC	2 cut(s)	1676, 2075
NmeAIII	GCCGAG	1 cut(s)	33
NmuCI	GTSAC	2 cut(s)	1177, 1483
NsiI	ATGCAT	2 cut(s)	592, 2912
NspI	RCATGY	4 cut(s)	183, 1839, 2327, 2910
PaeI	GCATGC	1 cut(s)	2910
PaqCI	CACCTGC	1 cut(s)	2659
PasI	CCCWGGG	1 cut(s)	1990
PauI	GCGCGC	1 cut(s)	1158
PciI	ACATGT	2 cut(s)	179, 2323
PciSI	GCTCTTC	1 cut(s)	2947
PctI	GAATGC	2 cut(s)	568, 2538
PdiI	GCCGGC	2 cut(s)	73, 2568
PdmI	GAANNNNTTC	6 cut(s)	456, 850, 1058, 1093, 1276, 2783
PfeI	GAWTC	4 cut(s)	86, 110, 2307, 2637
PfoI	TCCNGGA	2 cut(s)	105, 472
Ple19I	CGATCG	1 cut(s)	2685
PleI	GAGTC	4 cut(s)	18, 173, 485, 2639
PluTI	GGCGCC	2 cut(s)	8, 1560
PpsI	GAGTC	4 cut(s)	18, 173, 485, 2639
Ppu21I	YACGTR	2 cut(s)	2725, 2809
PscI	ACATGT	2 cut(s)	179, 2323
PshBI	ATTAAT	3 cut(s)	539, 792, 1614
Psp124BI	GAGCTC	2 cut(s)	1084, 1945
Psp6I	CCWGG	4 cut(s)	105, 472, 1417, 1989
PspFI	CCCAGC	1 cut(s)	332
PspGI	CCWGG	4 cut(s)	105, 472, 1417, 1989
PspOMI	GGGCCC	1 cut(s)	989
PspPI	GGNCC	6 cut(s)	267, 400, 989, 990, 2419, 2694
PstI	CTGCAG	2 cut(s)	219, 2071
PstNI	CAGNNNCTG	1 cut(s)	1181
PsuI	RGATCY	1 cut(s)	773
PteI	GCGCGC	1 cut(s)	1158
PvuI	CGATCG	1 cut(s)	2685
PvuII	CAGCTG	2 cut(s)	1073, 2717
RsaI	GTAC	6 cut(s)	391, 600, 1236, 1423, 1981, 2825
RsaNI	GTAC	6 cut(s)	390, 599, 1235, 1422, 1980, 2824
RseI	CAYNNNNRTG	2 cut(s)	2030, 2915
SacI	GAGCTC	2 cut(s)	1084, 1945
SapI	GCTCTTC	1 cut(s)	2947
SaqAI	TTAA	7 cut(s)	539, 792, 816, 1406, 1601, 1614, 2052
Sau96I	GGNCC	6 cut(s)	267, 400, 989, 990, 2419, 2694
SchI	GAGTC	4 cut(s)	19, 174, 486, 2639
ScrFI	CCNGG	8 cut(s)	107, 474, 1395, 1396, 1419, 1991, 2232, 2854
SduI	GDGCHC	6 cut(s)	214, 363, 993, 1084, 1849, 1945
SfcI	CTRYAG	6 cut(s)	215, 1317, 1695, 2067, 2148, 2520
SfoI	GGCGCC	2 cut(s)	6, 1558
SgrAI	CRCCGGYG	1 cut(s)	71
SinI	GGWCC	2 cut(s)	2419, 2694
SmaI	CCCGGG	1 cut(s)	1396
SmiMI	CAYNNNNRTG	2 cut(s)	2030, 2915
SmlI	CTYRAG	2 cut(s)	356, 2773
SmoI	CTYRAG	2 cut(s)	356, 2773
SphI	GCATGC	1 cut(s)	2910
SsiI	CCGC	8 cut(s)	42, 69, 140, 372, 956, 2417, 2888, 2897
SspDI	GGCGCC	2 cut(s)	4, 1556
SspI	AATATT	1 cut(s)	880
SspMI	CTAG	4 cut(s)	771, 1677, 2076, 2657
SstI	GAGCTC	2 cut(s)	1084, 1945
StyD4I	CCNGG	8 cut(s)	105, 472, 1393, 1394, 1417, 1989, 2230, 2852
StyI	CCWWGG	3 cut(s)	777, 1704, 2817
TaaI	ACNGT	4 cut(s)	1597, 1696, 2005, 2014
TaiI	ACGT	5 cut(s)	99, 2550, 2727, 2754, 2811
TaqI	TCGA	7 cut(s)	59, 194, 204, 282, 934, 2451, 2797
TaqII	GACCGA	1 cut(s)	2711
TatI	WGTACW	2 cut(s)	598, 1234
TauI	GCSGC	3 cut(s)	71, 2890, 2900
TfiI	GAWTC	4 cut(s)	86, 110, 2307, 2637
Tru1I	TTAA	7 cut(s)	539, 792, 816, 1406, 1601, 1614, 2052
Tru9I	TTAA	7 cut(s)	539, 792, 816, 1406, 1601, 1614, 2052
TseFI	GTSAC	2 cut(s)	1177, 1483
TseI	GCWGC	8 cut(s)	185, 431, 1070, 1073, 2438, 2660, 2714, 2849
Tsp45I	GTSAC	2 cut(s)	1177, 1483
TspDTI	ATGAA	6 cut(s)	161, 318, 919, 957, 1512, 2583
TspGWI	ACGGA	2 cut(s)	235, 1797
TspMI	CCCGGG	1 cut(s)	1394
VneI	GTGCAC	1 cut(s)	1845
VpaK11BI	GGWCC	2 cut(s)	2419, 2694
VspI	ATTAAT	3 cut(s)	539, 792, 1614
XagI	CCTNNNNNAGG	1 cut(s)	1988
XapI	RAATTY	9 cut(s)	121, 196, 741, 812, 1519, 1546, 1658, 2045, 2332
XbaI	TCTAGA	1 cut(s)	770
XceI	RCATGY	4 cut(s)	183, 1839, 2327, 2910
XcmI	CCANNNNNNNNNTGG	2 cut(s)	410, 1822
XmaI	CCCGGG	1 cut(s)	1394
XmiI	GTMKAC	1 cut(s)	1733
XmnI	GAANNNNTTC	6 cut(s)	456, 850, 1058, 1093, 1276, 2783
XspI	CTAG	4 cut(s)	771, 1677, 2076, 2657
Zsp2I	ATGCAT	2 cut(s)	592, 2912

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.