Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0009249

Physical Location & Seq

Genomic Coordinates Forward (+)

16389866 .. 16395882

Gene Structure 6017 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032554

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3006 bp

ATGGCGCCGAGTCTCAAGGACCTCCAGCTCACGCAGGTCTCGAAGTCCGGCCGCGGCGGCGGAGACGACGGCGGCGGTGAAGACCTCGAGGACGTGAGGCTTTTGGATTCGTATGAGAATTCGGAGGGAGTTGAAGAAGGGATGAGGACGGTTCAGGTCAGAGTTACCGGGATGACGTGTGCTGCTTGCTCTAATTCCGTTGAGGGAGCTCTTCAGAGCGTCAATGGCGTGCTCAGGGCCTCCGTGGCTTTGCTTCAGAATCGGGCTGACGTGGTTTTTGATCCCAGATTGGTCAAGGATGAAGACATCAAAAATGCAATTGAAGATGCTGGGTTTGAGGCAGAGGTTATACCTGAGCCCAGTGCAAACGGAGTAAAGCAGCATGGAAACATGTCCGGTCAGTTTTCGATAGGAGGTATGACATGTGCAGCTTGCGTGAACTCTGTTGAAGGTATTTTGAAAGGGCTTCCTGGGGTCAAAAGAGCTGTAGTAGCTTTAGCTACTTCACTAGGGGAAGTGGAGTACGATCCCTCTGTAATTAGTAAAGATGACATAGTCAATGCCATTGAAGATGCTGGTTTTGAAGCATCTTTTGTACAGAGTAGTGAGCAGGATAAAATTATACTGGGAGTTGCTGGCATATTCAGTGAGATAGATGCACAGGTTTTAGAAGCCATAATCAGCAACTTGAAAGGGGTGAGACATTTTCGTCTTGACAGGATTTCAAGAGAACTTGAAATCCTCTTTGATCCTGAAGTTGTCACTTCTAGATCCTTGGTTGATGGGATTGAGGGGGCTAGCAATGGGAAGTTCAAACTTCAAGTAGCAAACCCTTATACAAGAATGACTTGCAAAGATGCCGATGAAGCCTCAAATATGTTTCGGCTTTTCATTTCCAGCCTGGTTCTCAGTGTTCCTGTCTTTCTCATTCGAGTAGTCTGCCCTCACATTCCACTTCTGTATTCATTATTGCTCTGGAGATGTGGGCCTTTCGAAATGGGTGATTGGTTGAAGTGGGCATTGGTGAGTGTTGTTCAATTTGTGATTGGAAAGCGCTTCTACATTGCAGCTGCAAGAGCCCTACGAAATGGTTCAACTAACATGGATGTTCTAGTCGCCTTGGGAACTTCGGCCTCTTACTTCTACTCTGTTTGTGCACTTCTATATGGTGCAGTCACTGGGTTTTGGTCTCCTACATACTTTGAAACAAGTGCCATGCTAATAACATTTGTACTATTGGGGAAGTATCTGGAATGTCTTGCAAAAGGAAAGACATCAGATGCCATCAAAAAGTTGATAGAACTCGCTCCAGCTACAGCTTTGTTGCTTGTTAAAGATAAAGGTGGGAGATGTGTTGGAGAGAGGGAAATTGATGCTTTGCTCATTCAGCCTGGAGATACATTAAAAGTTCTTCCTGGTACAAAAGTTCCTGCTGATGGTATGGTTGTGTGGGGTTCAAGTTATGTAAATGAGAGTATGGTAACTGGGGAAGCTATACCTGTTTCAAAAGAAGTCAACACGTTGGTGATTGGAGGAACAATAAATTTACATGGTGCCCTTCATGTACAAGTTACAAAAGTTGGATCTGATACAGTTTTACATCAAATAATTAATTTGGTCGAGACCGCTCAAATGTCCAAAGCTCCTATTCAGAAATTTGCTGATTTTGTTGCAAGCATTTTTGTTCCTACTGTAGTTGTATTGTCATTGTTGACATTTTTGGGTTGGTATGTTGCTGGAGCCTTTGGAGCTTACCCAGAACAGTGGCTCCCTGAAAATGGAAATCACTTTGTTTTTGCCCTTATGTTTTCCATTTCAGTTGTGGTCATTGCATGCCCCTGTGCACTTGGCTTGGCAACACCAACAGCTGTCATGGTCGCAACAGGGGTTGGTGCTAACAATGGTGTGCTGATAAAAGGAGGAGATGCTCTGGAAAGAGCTCAAAAGGTTAAGTACGTGATATTCGATAAAACAGGCACCCTAACCCAAGGAAAGGCTACAGTTACTGCTGTAAAAGTTTTCACAGGAATGGATCGTGGAGATTTTCTTAAGTTGGTGGCATCTGCAGAGGCTAGCAGTGAACACCCACTAGGAAAAGCAATAGTTGAATATGCACGCCATTTCCACTTCTTTGATGAGCCTTCTGCAACCAATGATGCAACAAACCAAAGCAAGGAGCCCCTGATCTCTGGATGGCTTTTTGATGTTTCAGATTTCTCTGCTTTGCCAGGTAGAGGCATCCAGTGCTCAATAAATGGAAAACTTATTCTGGTTGGTAATAGGAAATTGATGACTGAAAGTGGAATCGACATTCCAACCCATGTTGAGGACTTTGTAGTAGAGCTGGAAGAAAGTGCAAAGACAGGCATACTTGTTGCATATGAAGGCAATTTAGTTGGTGTATTGGGGGTTGCAGACCCGCTAAAAAGAGAAGCTGCAATAGTCATAGAGGGACTTGGTAAGATGGGTGTCGTACCAGTCATGGTTACAGGAGATAATTGGAGGACAGCACAGGCTGTTGCAAAGGAGGTTGGCATTAAAGATGTGCGAGCGGAGGTAATGCCGGCTGGAAAAGCTGATGTTGTACGTTCATTCCAAAAAGATGGAAGCATAGTTGCAATGGTTGGTGATGGAATCAACGACTCTCCTGCCCTAGCTGCTTCTGATGTTGGTATGGCAATAGGGGCAGGGACAGATATTGCAATTGAAGCAGCTGACTATGTACTGATGAGAAATAACTTGGAAGATGTGATCACAGCCGTTGATCTCTCGAGAAAGACTTTCACTCGTATCCGATTGAATTATGTGTTTGCCATGGCCTACAATGTGATTGCAATACCTATTGCCGCAGGGGTTTTCTTTCCTTCACTGGGGATCATGTTGCCGCCATGGGTAGCCGGTGCATGCATGGCTATGTCATGTGTTAGTGTTGTATGCTCCTCTTTGCTCCTTAGGAGATACAGAAAACCCCGACTTACCGCCATACTGGAAATAGTTGTAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0012505 GO:0016020 GO:0023052 GO:0031410 GO:0031982 GO:0031984 GO:0032870 GO:0035556 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071369 GO:0071495 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1002

Amino Acids

106.88

Weight (kDa)

5.16

Isoelectric Point (pI)

29.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	53 - 113	6e-13	Heavy-metal-associated domain
HMA	PF00403	135 - 195	3.3e-15	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	437 - 541	2.8e-24	P-type ATPase actuator domain
Hydrolase	PF00702	650 - 888	1.2e-29	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44790
fragaria_vesca	FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710
malus_domestica	MD05G1247900.v1.1 MD10G1228800.v1.1
prunus_persica	Prupe.4G115900_v2.0.a1 Prupe.4G115900_v2.0.a1
pyrus_communis	pycom05g22420 pycom10g19240
rosa_chinensis	RchiOBHm_Chr5g0020701
rosa_laevigata	RLG00000032554
rosa_multiflora	Rmu_sc0005607.1_g000032
rosa_roxburghii	Rroxscaffold_1G00057600
rosa_rugosa	Rorug05G0062500
rosa_samantha	Rh5AG152200 Rh5BG151900 Rh5CG163300 Rh5DG151400
rosa_wichuraiana	Rw5G013490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	708
AbsI	CCTCGAGG	1 cut(s)	86
Acc36I	ACCTGC	1 cut(s)	25
AccB1I	GGYRCC	3 cut(s)	4, 1553, 1976
AccBSI	CCGCTC	2 cut(s)	1628, 2552
AccII	CGCG	1 cut(s)	54
AclWI	GGATC	7 cut(s)	275, 521, 743, 765, 1591, 2040, 2882
AcoI	YGGCCR	1 cut(s)	49
AcsI	RAATTY	3 cut(s)	118, 1543, 1655
AcuI	CTGAAG	3 cut(s)	197, 239, 774
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	9 cut(s)	524, 597, 1233, 1420, 1566, 1955, 2475, 2586, 2724
AfeI	AGCGCT	1 cut(s)	1055
AfiI	CCNNNNNNNGG	7 cut(s)	1436, 1778, 1993, 2173, 2234, 2326, 2870
AflII	CTTAAG	1 cut(s)	2048
AflIII	ACRYGT	4 cut(s)	176, 390, 422, 1518
AjiI	CACGTC	3 cut(s)	94, 177, 271
AjnI	CCWGG	5 cut(s)	469, 900, 1390, 1414, 2227
AjuI	GAANNNNNNNTTGG	2 cut(s)	1004, 1036
AleI	CACNNNNGTG	1 cut(s)	1523
AloI	GAACNNNNNNTCC	4 cut(s)	723, 755, 1752, 1784
Alw21I	GWGCWC	6 cut(s)	211, 234, 1159, 1846, 1942, 2249
Alw26I	GTCTC	6 cut(s)	17, 43, 57, 694, 1194, 1616
Alw44I	GTGCAC	2 cut(s)	1155, 1842
AlwI	GGATC	7 cut(s)	275, 521, 743, 765, 1591, 2040, 2882
AlwNI	CAGNNNCTG	2 cut(s)	1178, 2006
Ama87I	CYCGRG	2 cut(s)	86, 2770
Aor51HI	AGCGCT	1 cut(s)	1055
AoxI	GGCC	5 cut(s)	49, 237, 986, 1131, 2817
ApaLI	GTGCAC	2 cut(s)	1155, 1842
ApeKI	GCWGC	8 cut(s)	182, 379, 428, 1067, 1070, 2435, 2657, 2711
ApoI	RAATTY	3 cut(s)	118, 1543, 1655
ArsI	GACNNNNNNTTYG	2 cut(s)	260, 292
AseI	ATTAAT	1 cut(s)	1611
Asp700I	GAANNNNTTC	1 cut(s)	1090
AspLEI	GCGC	2 cut(s)	7, 1056
AspS9I	GGNCC	3 cut(s)	19, 237, 986
AsuC2I	CCSGG	1 cut(s)	169
AsuHPI	GGTGA	6 cut(s)	89, 709, 1013, 1036, 1537, 2639
AsuII	TTCGAA	1 cut(s)	993
AsuNHI	GCTAGC	2 cut(s)	797, 2072
AvaI	CYCGRG	2 cut(s)	86, 2770
AvaII	GGWCC	1 cut(s)	19
AxyI	CCTNAGG	1 cut(s)	2951
BaeGI	GKGCMC	3 cut(s)	1159, 1558, 1846
BaeI	ACNNNNGTAYC	2 cut(s)	1410, 1443
BanI	GGYRCC	3 cut(s)	4, 1553, 1976
BanII	GRGCYC	5 cut(s)	211, 360, 1081, 1942, 2181
BbsI	GAAGAC	2 cut(s)	87, 309
Bbv12I	GWGCWC	6 cut(s)	211, 234, 1159, 1846, 1942, 2249
BbvI	GCAGC	8 cut(s)	169, 391, 440, 1057, 1079, 2422, 2644, 2723
BccI	CCATC	7 cut(s)	776, 1292, 1430, 2187, 2458, 2597, 2624
BceAI	ACGGC	2 cut(s)	85, 2744
BcgI	CGANNNNNNTGC	4 cut(s)	921, 955, 2630, 2664
BciT130I	CCWGG	5 cut(s)	471, 902, 1392, 1416, 2229
BciVI	GTATCC	1 cut(s)	2801
BclI	TGATCA	1 cut(s)	2751
BcnI	CCSGG	1 cut(s)	169
BcoDI	GTCTC	6 cut(s)	17, 43, 57, 694, 1194, 1616
BfaI	CTAG	7 cut(s)	509, 768, 798, 1112, 2073, 2090, 2654
BfmI	CTRYAG	5 cut(s)	486, 1314, 1692, 1998, 2064
BfoI	RGCGCY	2 cut(s)	8, 1057
BfrI	CTTAAG	1 cut(s)	2048
BfuAI	ACCTGC	1 cut(s)	25
BfuI	GTATCC	1 cut(s)	2801
BglI	GCCNNNNNGGC	2 cut(s)	57, 245
Bme1390I	CCNGG	6 cut(s)	169, 471, 902, 1392, 1416, 2229
Bme18I	GGWCC	1 cut(s)	19
BmeT110I	CYCGRG	2 cut(s)	86, 2770
BmgBI	CACGTC	3 cut(s)	94, 177, 271
BmgT120I	GGNCC	3 cut(s)	19, 237, 986
BmiI	GGNNCC	6 cut(s)	6, 1555, 1741, 1769, 1978, 2178
BmrFI	CCNGG	6 cut(s)	169, 471, 902, 1392, 1416, 2229
BmrI	ACTGGG	5 cut(s)	354, 635, 1188, 1494, 2879
BmtI	GCTAGC	2 cut(s)	801, 2076
BmuI	ACTGGG	5 cut(s)	354, 635, 1188, 1494, 2879
BpiI	GAAGAC	2 cut(s)	87, 309
BpmI	CTGGAG	5 cut(s)	8, 997, 1293, 1413, 1758
Bpu10I	CCTNAGC	2 cut(s)	233, 354
Bpu14I	TTCGAA	1 cut(s)	993
BpuMI	CCSGG	1 cut(s)	169
BsaAI	YACGTR	1 cut(s)	1957
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	3 cut(s)	43, 1194, 1616
BsaJI	CCNNGG	8 cut(s)	52, 243, 470, 774, 1119, 1987, 2814, 2888
BsaWI	WCCGGW	1 cut(s)	395
BsaXI	ACNNNNNCTCC	2 cut(s)	1752, 1782
Bsc4I	CCNNNNNNNGG	7 cut(s)	1436, 1778, 1993, 2173, 2234, 2326, 2870
Bse118I	RCCGGY	2 cut(s)	2563, 2897
Bse1I	ACTGG	8 cut(s)	360, 630, 1183, 1489, 2242, 2477, 2874, 2991
Bse21I	CCTNAGG	1 cut(s)	2951
Bse3DI	GCAATG	4 cut(s)	808, 1062, 1827, 2625
BseBI	CCWGG	5 cut(s)	471, 902, 1392, 1416, 2229
BseDI	CCNNGG	8 cut(s)	52, 243, 470, 774, 1119, 1987, 2814, 2888
BseGI	GGATG	6 cut(s)	147, 177, 304, 1111, 2198, 2238
BseLI	CCNNNNNNNGG	7 cut(s)	1436, 1778, 1993, 2173, 2234, 2326, 2870
BseMI	GCAATG	4 cut(s)	808, 1062, 1827, 2625
BseMII	CTCAG	3 cut(s)	247, 345, 922
BseNI	ACTGG	8 cut(s)	360, 630, 1183, 1489, 2242, 2477, 2874, 2991
BseRI	GAGGAG	2 cut(s)	1935, 2929
BseSI	GKGCMC	3 cut(s)	1159, 1558, 1846
BseX3I	CGGCCG	1 cut(s)	49
BseXI	GCAGC	8 cut(s)	169, 391, 440, 1057, 1079, 2422, 2644, 2723
BseYI	CCCAGC	1 cut(s)	329
BsgI	GTGCAG	2 cut(s)	447, 1191
Bsh1236I	CGCG	1 cut(s)	54
Bsh1285I	CGRYCG	1 cut(s)	52
BshFI	GGCC	5 cut(s)	51, 239, 988, 1133, 2819
BshNI	GGYRCC	3 cut(s)	4, 1553, 1976
BsiEI	CGRYCG	1 cut(s)	52
BsiHKAI	GWGCWC	6 cut(s)	211, 234, 1159, 1846, 1942, 2249
BsiHKCI	CYCGRG	2 cut(s)	86, 2770
BsiSI	CCGG	5 cut(s)	48, 168, 396, 2564, 2898
BslFI	GGGAC	2 cut(s)	2466, 2704
BslI	CCNNNNNNNGG	7 cut(s)	1436, 1778, 1993, 2173, 2234, 2326, 2870
BsmAI	GTCTC	6 cut(s)	17, 43, 57, 694, 1194, 1616
BsmBI	CGTCTC	1 cut(s)	57
BsmFI	GGGAC	2 cut(s)	2466, 2704
BsnI	GGCC	5 cut(s)	51, 239, 988, 1133, 2819
Bso31I	GGTCTC	3 cut(s)	43, 1194, 1616
BsoBI	CYCGRG	2 cut(s)	86, 2770
Bsp119I	TTCGAA	1 cut(s)	993
Bsp1407I	TGTACA	2 cut(s)	595, 1564
Bsp19I	CCATGG	2 cut(s)	2814, 2888
BspANI	GGCC	5 cut(s)	51, 239, 988, 1133, 2819
BspCNI	CTCAG	3 cut(s)	246, 346, 921
BspFNI	CGCG	1 cut(s)	54
BspLI	GGNNCC	6 cut(s)	6, 1555, 1741, 1769, 1978, 2178
BspMAI	CTGCAG	1 cut(s)	2068
BspMI	ACCTGC	1 cut(s)	25
BspOI	GCTAGC	2 cut(s)	801, 2076
BspPI	GGATC	7 cut(s)	275, 521, 743, 765, 1591, 2040, 2882
BspQI	GCTCTTC	1 cut(s)	216
BspT104I	TTCGAA	1 cut(s)	993
BspT107I	GGYRCC	3 cut(s)	4, 1553, 1976
BspTI	CTTAAG	1 cut(s)	2048
BspTNI	GGTCTC	3 cut(s)	43, 1194, 1616
BsrBI	CCGCTC	2 cut(s)	1628, 2552
BsrDI	GCAATG	4 cut(s)	808, 1062, 1827, 2625
BsrFI	RCCGGY	2 cut(s)	2563, 2897
BsrGI	TGTACA	2 cut(s)	595, 1564
BsrI	ACTGG	8 cut(s)	360, 630, 1183, 1489, 2242, 2477, 2874, 2991
BssAI	RCCGGY	2 cut(s)	2563, 2897
BssECI	CCNNGG	8 cut(s)	52, 243, 470, 774, 1119, 1987, 2814, 2888
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	5 cut(s)	774, 1119, 1987, 2814, 2888
Bst2UI	CCWGG	5 cut(s)	471, 902, 1392, 1416, 2229
Bst4CI	ACNGT	5 cut(s)	151, 1594, 1693, 1764, 2002
Bst6I	CTCTTC	1 cut(s)	216
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	2048
BstAPI	GCANNNNNTGC	1 cut(s)	2244
BstAUI	TGTACA	2 cut(s)	595, 1564
BstBAI	YACGTR	1 cut(s)	1957
BstBI	TTCGAA	1 cut(s)	993
BstDEI	CTNAG	4 cut(s)	233, 354, 908, 2951
BstDSI	CCRYGG	4 cut(s)	52, 243, 2814, 2888
BstF5I	GGATG	6 cut(s)	147, 177, 304, 1111, 2198, 2238
BstFNI	CGCG	1 cut(s)	54
BstH2I	RGCGCY	2 cut(s)	8, 1057
BstHHI	GCGC	2 cut(s)	7, 1056
BstMAI	GTCTC	6 cut(s)	17, 43, 57, 694, 1194, 1616
BstMCI	CGRYCG	1 cut(s)	52
BstNI	CCWGG	5 cut(s)	471, 902, 1392, 1416, 2229
BstNSI	RCATGY	4 cut(s)	394, 426, 1836, 2907
BstSCI	CCNGG	6 cut(s)	167, 469, 900, 1390, 1414, 2227
BstSFI	CTRYAG	5 cut(s)	486, 1314, 1692, 1998, 2064
BstSLI	GKGCMC	3 cut(s)	1159, 1558, 1846
BstUI	CGCG	1 cut(s)	54
BstV1I	GCAGC	8 cut(s)	169, 391, 440, 1057, 1079, 2422, 2644, 2723
BstV2I	GAAGAC	2 cut(s)	87, 309
BstX2I	RGATCY	2 cut(s)	770, 1583
BstXI	CCANNNNNNTGG	2 cut(s)	1764, 2603
BstYI	RGATCY	2 cut(s)	770, 1583
BstZI	CGGCCG	1 cut(s)	49
Bsu36I	CCTNAGG	1 cut(s)	2951
BsuI	GTATCC	1 cut(s)	2801
BsuRI	GGCC	5 cut(s)	51, 239, 988, 1133, 2819
BtgI	CCRYGG	4 cut(s)	52, 243, 2814, 2888
BtrI	CACGTC	3 cut(s)	94, 177, 271
BtsCI	GGATG	6 cut(s)	147, 177, 304, 1111, 2198, 2238
BtsI	GCAGTG	1 cut(s)	2083
BtsIMutI	CAGTG	8 cut(s)	367, 652, 916, 1176, 1769, 2083, 2249, 2867
BveI	ACCTGC	1 cut(s)	25
CaiI	CAGNNNCTG	2 cut(s)	1178, 2006
CfoI	GCGC	2 cut(s)	7, 1056
Cfr10I	RCCGGY	2 cut(s)	2563, 2897
Cfr13I	GGNCC	3 cut(s)	19, 237, 986
Cfr42I	CCGCGG	1 cut(s)	55
CseI	GACGC	1 cut(s)	208
Csp6I	GTAC	9 cut(s)	523, 596, 1232, 1419, 1565, 1954, 2474, 2585, 2723
CviQI	GTAC	9 cut(s)	523, 596, 1232, 1419, 1565, 1954, 2474, 2585, 2723
DdeI	CTNAG	4 cut(s)	233, 354, 908, 2951
DinI	GGCGCC	1 cut(s)	6
DrdI	GACNNNNNNGTC	1 cut(s)	708
DseDI	GACNNNNNNGTC	1 cut(s)	708
EaeI	YGGCCR	1 cut(s)	49
EagI	CGGCCG	1 cut(s)	49
Eam1104I	CTCTTC	1 cut(s)	216
EarI	CTCTTC	1 cut(s)	216
EciI	GGCGGA	1 cut(s)	75
Ecl136II	GAGCTC	2 cut(s)	209, 1940
EclXI	CGGCCG	1 cut(s)	49
Eco130I	CCWWGG	5 cut(s)	774, 1119, 1987, 2814, 2888
Eco24I	GRGCYC	5 cut(s)	211, 360, 1081, 1942, 2181
Eco31I	GGTCTC	3 cut(s)	43, 1194, 1616
Eco47I	GGWCC	1 cut(s)	19
Eco47III	AGCGCT	1 cut(s)	1055
Eco52I	CGGCCG	1 cut(s)	49
Eco53kI	GAGCTC	2 cut(s)	209, 1940
Eco57I	CTGAAG	3 cut(s)	197, 239, 774
Eco81I	CCTNAGG	1 cut(s)	2951
Eco88I	CYCGRG	2 cut(s)	86, 2770
EcoICRI	GAGCTC	2 cut(s)	209, 1940
EcoO109I	RGGNCCY	2 cut(s)	19, 237
EcoRI	GAATTC	1 cut(s)	118
EcoRII	CCWGG	5 cut(s)	469, 900, 1390, 1414, 2227
EcoT14I	CCWWGG	5 cut(s)	774, 1119, 1987, 2814, 2888
EcoT22I	ATGCAT	1 cut(s)	2909
EcoT38I	GRGCYC	5 cut(s)	211, 360, 1081, 1942, 2181
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	5 cut(s)	774, 1119, 1987, 2814, 2888
Esp3I	CGTCTC	1 cut(s)	57
FalI	AAGNNNNNCTT	2 cut(s)	832, 864
FaqI	GGGAC	2 cut(s)	2466, 2704
FauI	CCCGC	1 cut(s)	2427
FauNDI	CATATG	1 cut(s)	2380
FbaI	TGATCA	1 cut(s)	2751
FokI	GGATG	6 cut(s)	154, 184, 311, 1118, 2205, 2225
FriOI	GRGCYC	5 cut(s)	211, 360, 1081, 1942, 2181
FspBI	CTAG	7 cut(s)	509, 768, 798, 1112, 2073, 2090, 2654
GlaI	GCGC	2 cut(s)	6, 1055
GsaI	CCCAGC	1 cut(s)	333
GsuI	CTGGAG	5 cut(s)	8, 997, 1293, 1413, 1758
HaeII	RGCGCY	2 cut(s)	8, 1057
HaeIII	GGCC	5 cut(s)	51, 239, 988, 1133, 2819
HapII	CCGG	5 cut(s)	48, 168, 396, 2564, 2898
HgaI	GACGC	1 cut(s)	208
HhaI	GCGC	2 cut(s)	7, 1056
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	2 cut(s)	5, 1054
HinP1I	GCGC	2 cut(s)	5, 1054
HincII	GTYRAC	2 cut(s)	1516, 1713
HindII	GTYRAC	2 cut(s)	1516, 1713
HinfI	GANTC	6 cut(s)	10, 107, 259, 2304, 2634, 2642
HpaII	CCGG	5 cut(s)	48, 168, 396, 2564, 2898
HphI	GGTGA	6 cut(s)	89, 709, 1013, 1036, 1537, 2639
Hpy166II	GTNNAC	6 cut(s)	439, 1157, 1516, 1713, 1844, 2081
Hpy8I	GTNNAC	6 cut(s)	439, 1157, 1516, 1713, 1844, 2081
Hpy99I	CGWCG	1 cut(s)	71
HpyAV	CCTTC	6 cut(s)	131, 443, 1568, 2151, 2378, 2874
HpyCH4III	ACNGT	5 cut(s)	151, 1594, 1693, 1764, 2002
HpyCH4IV	ACGT	6 cut(s)	93, 176, 270, 1520, 1956, 2587
HpyF3I	CTNAG	4 cut(s)	233, 354, 908, 2951
HpySE526I	ACGT	6 cut(s)	93, 176, 270, 1520, 1956, 2587
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	2 cut(s)	5, 1054
KasI	GGCGCC	1 cut(s)	4
KroI	GCCGGC	1 cut(s)	2563
KroNI	GCCGGC	1 cut(s)	2565
Ksp22I	TGATCA	1 cut(s)	2751
KspI	CCGCGG	1 cut(s)	55
LguI	GCTCTTC	1 cut(s)	216
LmnI	GCTCC	9 cut(s)	206, 1312, 1648, 1739, 1748, 1773, 2176, 2942, 2952
Lsp1109I	GCAGC	8 cut(s)	169, 391, 440, 1057, 1079, 2422, 2644, 2723
MaeI	CTAG	7 cut(s)	509, 768, 798, 1112, 2073, 2090, 2654
MaeII	ACGT	6 cut(s)	93, 176, 270, 1520, 1956, 2587
MaeIII	GTNAC	7 cut(s)	163, 760, 1174, 1480, 1570, 2002, 2485
MbiI	CCGCTC	2 cut(s)	1628, 2552
MboII	GAAGA	9 cut(s)	92, 146, 203, 314, 335, 581, 1403, 2360, 2756
MfeI	CAATTG	2 cut(s)	318, 2703
MflI	RGATCY	2 cut(s)	770, 1583
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	19, 2636
MmeI	TCCRAC	3 cut(s)	1336, 1561, 2339
Mph1103I	ATGCAT	1 cut(s)	2909
MroNI	GCCGGC	1 cut(s)	2563
MroXI	GAANNNNTTC	1 cut(s)	1090
MseI	TTAA	6 cut(s)	1332, 1403, 1611, 1950, 2049, 2538
MslI	CAYNNNNRTG	3 cut(s)	1523, 2027, 2912
MspA1I	CMGCKG	4 cut(s)	54, 1070, 1868, 2714
MspCI	CTTAAG	1 cut(s)	2048
MspI	CCGG	5 cut(s)	48, 168, 396, 2564, 2898
MspR9I	CCNGG	6 cut(s)	169, 471, 902, 1392, 1416, 2229
MunI	CAATTG	2 cut(s)	318, 2703
MvaI	CCWGG	5 cut(s)	471, 902, 1392, 1416, 2229
MvnI	CGCG	1 cut(s)	54
NaeI	GCCGGC	1 cut(s)	2565
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	1 cut(s)	169
NcoI	CCATGG	2 cut(s)	2814, 2888
NdeI	CATATG	1 cut(s)	2380
NgoMIV	GCCGGC	1 cut(s)	2563
NheI	GCTAGC	2 cut(s)	797, 2072
NlaIV	GGNNCC	6 cut(s)	6, 1555, 1741, 1769, 1978, 2178
NmeAIII	GCCGAG	1 cut(s)	33
NmuCI	GTSAC	2 cut(s)	760, 1174
NsiI	ATGCAT	1 cut(s)	2909
NspI	RCATGY	4 cut(s)	394, 426, 1836, 2907
NspV	TTCGAA	1 cut(s)	993
OliI	CACNNNNGTG	1 cut(s)	1523
PaeI	GCATGC	2 cut(s)	1836, 2907
PaeR7I	CTCGAG	2 cut(s)	86, 2770
PciI	ACATGT	2 cut(s)	390, 422
PciSI	GCTCTTC	1 cut(s)	216
PcsI	WCGNNNNNNNCGW	2 cut(s)	38, 1962
PdiI	GCCGGC	1 cut(s)	2565
PdmI	GAANNNNTTC	1 cut(s)	1090
PfeI	GAWTC	4 cut(s)	107, 259, 2304, 2634
PflFI	GACNNNGTC	1 cut(s)	554
PleI	GAGTC	2 cut(s)	18, 2636
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	2 cut(s)	18, 2636
Ppu21I	YACGTR	1 cut(s)	1957
PpuMI	RGGWCCY	1 cut(s)	19
PscI	ACATGT	2 cut(s)	390, 422
PshBI	ATTAAT	1 cut(s)	1611
Psp124BI	GAGCTC	2 cut(s)	211, 1942
Psp5II	RGGWCCY	1 cut(s)	19
Psp6I	CCWGG	5 cut(s)	469, 900, 1390, 1414, 2227
PspFI	CCCAGC	1 cut(s)	329
PspGI	CCWGG	5 cut(s)	469, 900, 1390, 1414, 2227
PspN4I	GGNNCC	6 cut(s)	6, 1555, 1741, 1769, 1978, 2178
PspPI	GGNCC	3 cut(s)	19, 237, 986
PspPPI	RGGWCCY	1 cut(s)	19
PspXI	VCTCGAGB	1 cut(s)	86
PstI	CTGCAG	1 cut(s)	2068
PstNI	CAGNNNCTG	2 cut(s)	1178, 2006
PsuI	RGATCY	2 cut(s)	770, 1583
PsyI	GACNNNGTC	1 cut(s)	554
PvuII	CAGCTG	3 cut(s)	1070, 1868, 2714
RsaI	GTAC	9 cut(s)	524, 597, 1233, 1420, 1566, 1955, 2475, 2586, 2724
RsaNI	GTAC	9 cut(s)	523, 596, 1232, 1419, 1565, 1954, 2474, 2585, 2723
RseI	CAYNNNNRTG	3 cut(s)	1523, 2027, 2912
SacI	GAGCTC	2 cut(s)	211, 1942
SacII	CCGCGG	1 cut(s)	55
SapI	GCTCTTC	1 cut(s)	216
SaqAI	TTAA	6 cut(s)	1332, 1403, 1611, 1950, 2049, 2538
Sau96I	GGNCC	3 cut(s)	19, 237, 986
SchI	GAGTC	2 cut(s)	19, 2636
ScrFI	CCNGG	6 cut(s)	169, 471, 902, 1392, 1416, 2229
SfcI	CTRYAG	5 cut(s)	486, 1314, 1692, 1998, 2064
SfoI	GGCGCC	1 cut(s)	6
Sfr274I	CTCGAG	2 cut(s)	86, 2770
Sfr303I	CCGCGG	1 cut(s)	55
SfuI	TTCGAA	1 cut(s)	993
SgrBI	CCGCGG	1 cut(s)	55
SinI	GGWCC	1 cut(s)	19
SlaI	CTCGAG	2 cut(s)	86, 2770
SmiMI	CAYNNNNRTG	3 cut(s)	1523, 2027, 2912
SmlI	CTYRAG	4 cut(s)	14, 86, 2048, 2770
SmoI	CTYRAG	4 cut(s)	14, 86, 2048, 2770
SphI	GCATGC	2 cut(s)	1836, 2907
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	7 cut(s)	509, 768, 798, 1112, 2073, 2090, 2654
SstI	GAGCTC	2 cut(s)	211, 1942
StyD4I	CCNGG	6 cut(s)	167, 469, 900, 1390, 1414, 2227
StyI	CCWWGG	5 cut(s)	774, 1119, 1987, 2814, 2888
TaaI	ACNGT	5 cut(s)	151, 1594, 1693, 1764, 2002
TaiI	ACGT	6 cut(s)	96, 179, 273, 1523, 1959, 2590
TaqI	TCGA	9 cut(s)	41, 87, 407, 931, 993, 1620, 1965, 2307, 2771
TatI	WGTACW	4 cut(s)	595, 1231, 1564, 2722
TauI	GCSGC	6 cut(s)	54, 57, 60, 75, 2849, 2887
TfiI	GAWTC	4 cut(s)	107, 259, 2304, 2634
Tru1I	TTAA	6 cut(s)	1332, 1403, 1611, 1950, 2049, 2538
Tru9I	TTAA	6 cut(s)	1332, 1403, 1611, 1950, 2049, 2538
TscAI	CASTG	8 cut(s)	367, 652, 916, 1183, 1769, 2083, 2249, 2874
TseFI	GTSAC	2 cut(s)	760, 1174
TseI	GCWGC	8 cut(s)	182, 379, 428, 1067, 1070, 2435, 2657, 2711
Tsp45I	GTSAC	2 cut(s)	760, 1174
TspDTI	ATGAA	7 cut(s)	315, 879, 880, 954, 1550, 2397, 2580
TspGWI	ACGGA	3 cut(s)	187, 232, 384
TspRI	CASTG	8 cut(s)	367, 652, 916, 1183, 1769, 2083, 2249, 2874
Tth111I	GACNNNGTC	1 cut(s)	554
Vha464I	CTTAAG	1 cut(s)	2048
VneI	GTGCAC	2 cut(s)	1155, 1842
VpaK11BI	GGWCC	1 cut(s)	19
VspI	ATTAAT	1 cut(s)	1611
XapI	RAATTY	3 cut(s)	118, 1543, 1655
XbaI	TCTAGA	1 cut(s)	767
XceI	RCATGY	4 cut(s)	394, 426, 1836, 2907
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1819
XhoI	CTCGAG	2 cut(s)	86, 2770
XmnI	GAANNNNTTC	1 cut(s)	1090
XspI	CTAG	7 cut(s)	509, 768, 798, 1112, 2073, 2090, 2654
Zsp2I	ATGCAT	1 cut(s)	2909

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.