Rh5CG163300

Copper-transporting ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0009249
Physical Location & Seq
Forward (+)
15129018 .. 15135019
6002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG163300.1

Sequence Viewer

Length: 3009 bp
ATGGCGCCGAGTCTCAGGGACCTCCAGCTCACGCAGGTCTCCAACTCCGGCCGCGGCGGCGGCGGAGACGGCGGCGGCGCTGAAGACCTCGAGGACGTGAGGCTTTTGGATTCGTATGAGAATTCGGAGGGAGTTGAAGAAGGGATGAGGACGGTTCAGGTCAGAGTTACCGGGATGACGTGTGCTGCTTGCTCTAATTCGGTTGAGGGAGCTCTTCAGAGCGTCAATGGCGTGCTCAGGGCCTCCGTGGCGTTGCTTCAGAATCGGGCTGAAGTGGTTTTTGATCCCAGATTGGTCAAGGACGAAGACATCAAAAATGCAATTGAAGATGCTGGGTTTGAGGCAGAGGTTATACCTGAGCCCAGTGCAAACGGAGTAAAGCAGCATGGCAACCTGTCCGGTCAGTTTTCGATAGGAGGTATGACATGTGCAGCTTGCGTGAACTCTGTTGAAGGTATTCTGAAAGGGCTTCCTGGGGTCAAAAGAGCTGTAGTAGCTTTAGCTACTTCACTAGGGGAAGTGGAGTATGATCCTACTGTAATTAGTAAAGATGACATAGTCAATGCCATTGAAGATGCTGGTTTTGAAGCATCTTTTGTACAGAGTAGTGAGCAGGATAAAATTATACTGGGAGTTGCTGGCATATTCAGTGAGATAGATGCACAGGTTTTAGAAGGCATAATCAGCAACTTGAAAGGGGTGAGACATTTTCGTCTTGACAGGATTTCAAGAGAACTTGAAATCCTCTTTGATCCTGAAGTTGTCACTTCTAGATCCTTGGTTGATGGGATTGAGGGGGCTAGCATTGGGAAGTTCAAACTTCAAGTAGCAAACCCTTATACAAGAATGACTTGCAAAGATGTTGATGAAGCCTCAAATATGTTTCGGCTTTTCATTTCCAGCCTGGTTCTCAGTGTTCCTGTCTTTCTCATTCGAGTAGTCTGCCCTCACATTCCACTTCTGTATTCATTATTGCTCTGGAGATGTGGGCCTTTCGAAATGGGTGATTGGTTGAAGTGGGCATTGGTGAGTGTTGTTCAATTTGTGATTGGAAAGCGCTTCTACATTGCAGCTGCAAGAGCCCTACGAAATGGTTCAACTAACATGGATGTTCTGGTCGCCTTGGGAACTTCGGCCTCTTACTTCTACTCTGTTTGTGCACTTCTATATGGTGCAGTCACTGGGTTTTGGTCTCCTACATACTTTGAAACAAGTGCCATGCTAATAACATTTGTACTATTGGGGAAGTATCTGGAATGTCTTGCAAAAGGAAAGACATCAGATGCCATCAAAAAGTTGATAGAACTCACTCCAGCTACAGCTTTGTTGCTTGTTAAAGATAAGGGTGGGAGATGTGTTGGAGAGAGGGAAATTGATGCTTTGCTCATTCAGCCTGGAGATACGTTAAAAGTTCTTCCTGGTACAAAAGTTCCTGCTGATGGTATGGTTGTGTGGGGTTCAAGTTATGTAAATGAGAGTATGGTAACTGGGGAAGCTATACCTGTTTCAAAAGAAGTCAACACGTTGGTGATTGGAGGAACAATAAATTTACATGGTGCCCTTCACGTACAAGTTACAAAAGTTGGATCTGATACAGTTTTACATCAAATAATTAATTTGGTCGAGACCGCTCAAATGTCCAAAGCTCCTATTCAGAAATTTGCTGATTTTGTTGCAAGCATTTTTGTTCCTACTGTAGTTGTATTGTCATTGTTGACATTTTTGGGTTGGTATGTTGCTGGAGCCTTTGGAGCTTACCCAGAACAGTGGCTCCCTGAAAATGGAAATCACTTTGTTTTTGCCCTTATGTTTTCCATATCAGTTGTGGTCATTGCATGCCCCTGTGCACTTGGCTTGGCAACACCAACAGCTGTCATGGTCGCAACAGGGGTTGGCGCTAATAATGGTGTGCTGATAAAAGGAGGAGATGCTCTGGAAAGAGCTCAAAAGGTTAAGTACGTGATATTTGATAAAACAGGCACCCTAACCCAAGGAAAGGCTACAGTTACTGCTGTAAAAGTTTTCACAGGAATGGATCGTGGAGATTTTCTTAAGTTGGTGGCATCTGCAGAGGCTAGCAGTGAACACCCACTAGGAAAAGCAATAGTTGAATATGCACGCCATTTCCACTTCTTTGATGAGCCTTCTGCAACCAATGATGCAACAAACCAAAGCAAGGAGCCCCTGATATCTGGATGGCTTTTTGATGTCTCAGATTTCTCTGCTTTGCCAGGTAGAGGCATCCAGTGCTCAATAAATGGAAAACTTATTCTGGTTGGTAATAGGAAATTGATGACTGAAAGCGGAATCGACATTCCAACCCATGTTGAGGACTTTGTAGTAGAGCTGGAAGAAAGTGCAAAGACAGGCATACTTGTTGCATATGAAGGCAATTTAGTTGGTGTATTGGGGGTTGCAGACCCACTAAAAAGAGAAGCTGCAATAGTCATAGAGGGACTTGGTAAGATGGGTGTCATACCAGTCATGGTTACAGGAGATAATTGGAGGACAGCACAGGCTGTTGCAAAGGAGGTTGGCATTAAAGATGTGCGAGCGGAGGTAATGCCGGCTGGAAAAGCTGATGTTGTACGTTCATTCCAAAAAGATGGAAGCATAGTTGCAATGGTTGGTGATGGAATCAACGACTCTCCTGCCCTAGCTGCTTCTGATGTTGGTATGGCAATAGGGGCAGGGACAGATATTGCAATTGAAGCAGCTGACTATGTATTGATGAGAAATAACTTGGAAGATGTGATCACAGCCGTTGATCTCTCGAGAAAGACTTTCACTCGTATCCGATTGAATTATGTGTTTGCCATGGCCTACAATGTGATTGCAATACCTATTGCCGCAGGGGTTTTCTTTCCTTCACTGGGGATCATGCTGCCGCCATGGGTAGCCGGTGCATGCATGGCTATGTCATCTGTTAGTGTTGTATGCTCCTCTTTGCTCCTTAGGAGATACAGAAAACCCCGACTTACCGCCATACTGGAAATAGTTGTAGAGTAG

Protein Analysis

1002

Amino Acids

106.96

Weight (kDa)

5.16

Isoelectric Point (pI)

28.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 54 - 114 3.5e-13 Heavy-metal-associated domain
HMA PF00403 136 - 196 2.2e-15 Heavy-metal-associated domain
E1-E2_ATPase PF00122 437 - 542 2.4e-24 P-type ATPase actuator domain
Hydrolase PF00702 651 - 889 1.6e-29 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 711
AbsI CCTCGAGG 1 cut(s) 89
Acc36I ACCTGC 1 cut(s) 25
AccB1I GGYRCC 3 cut(s) 4, 1556, 1979
AccBSI CCGCTC 2 cut(s) 1631, 2555
AccII CGCG 1 cut(s) 54
AclWI GGATC 7 cut(s) 278, 524, 746, 768, 1594, 2043, 2885
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 3 cut(s) 121, 1546, 1658
AcuI CTGAAG 5 cut(s) 102, 200, 242, 291, 777
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 6 cut(s) 600, 1236, 1423, 1569, 1958, 2589
AfeI AGCGCT 1 cut(s) 1058
AfiI CCNNNNNNNGG 7 cut(s) 1439, 1781, 1996, 2176, 2237, 2329, 2873
AflII CTTAAG 1 cut(s) 2051
AflIII ACRYGT 3 cut(s) 179, 425, 1521
AjiI CACGTC 2 cut(s) 97, 180
AjnI CCWGG 5 cut(s) 472, 903, 1393, 1417, 2230
AjuI GAANNNNNNNTTGG 2 cut(s) 1007, 1039
AleI CACNNNNGTG 1 cut(s) 1526
AloI GAACNNNNNNTCC 4 cut(s) 726, 758, 1755, 1787
Alw21I GWGCWC 6 cut(s) 214, 237, 1162, 1849, 1945, 2252
Alw26I GTCTC 7 cut(s) 17, 43, 60, 697, 1197, 1619, 2215
Alw44I GTGCAC 2 cut(s) 1158, 1845
AlwI GGATC 7 cut(s) 278, 524, 746, 768, 1594, 2043, 2885
AlwNI CAGNNNCTG 2 cut(s) 1181, 2009
Ama87I CYCGRG 2 cut(s) 89, 2773
Aor51HI AGCGCT 1 cut(s) 1058
AoxI GGCC 5 cut(s) 49, 240, 989, 1134, 2820
ApaLI GTGCAC 2 cut(s) 1158, 1845
ApeKI GCWGC 9 cut(s) 185, 382, 431, 1070, 1073, 2438, 2660, 2714, 2884
ApoI RAATTY 3 cut(s) 121, 1546, 1658
AseI ATTAAT 1 cut(s) 1614
Asp700I GAANNNNTTC 2 cut(s) 456, 1093
AspLEI GCGC 4 cut(s) 7, 80, 1059, 1898
AspS9I GGNCC 3 cut(s) 19, 240, 989
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 5 cut(s) 712, 1016, 1039, 1540, 2642
AsuII TTCGAA 1 cut(s) 996
AsuNHI GCTAGC 2 cut(s) 800, 2075
AvaI CYCGRG 2 cut(s) 89, 2773
AvaII GGWCC 1 cut(s) 19
AxyI CCTNAGG 1 cut(s) 2954
BaeGI GKGCMC 3 cut(s) 1162, 1561, 1849
BaeI ACNNNNGTAYC 2 cut(s) 1413, 1446
BanI GGYRCC 3 cut(s) 4, 1556, 1979
BanII GRGCYC 5 cut(s) 214, 363, 1084, 1945, 2184
BbsI GAAGAC 2 cut(s) 90, 312
Bbv12I GWGCWC 6 cut(s) 214, 237, 1162, 1849, 1945, 2252
BbvI GCAGC 9 cut(s) 172, 394, 443, 1060, 1082, 2425, 2647, 2726, 2871
BccI CCATC 7 cut(s) 779, 1295, 1433, 2190, 2461, 2600, 2627
BceAI ACGGC 2 cut(s) 85, 2747
BcgI CGANNNNNNTGC 4 cut(s) 924, 958, 2633, 2667
BciT130I CCWGG 5 cut(s) 474, 905, 1395, 1419, 2232
BciVI GTATCC 1 cut(s) 2804
BclI TGATCA 1 cut(s) 2754
BcnI CCSGG 1 cut(s) 172
BcoDI GTCTC 7 cut(s) 17, 43, 60, 697, 1197, 1619, 2215
BfaI CTAG 6 cut(s) 512, 771, 801, 2076, 2093, 2657
BfmI CTRYAG 5 cut(s) 489, 1317, 1695, 2001, 2067
BfoI RGCGCY 4 cut(s) 8, 81, 1060, 1899
BfrI CTTAAG 1 cut(s) 2051
BfuAI ACCTGC 1 cut(s) 25
BfuI GTATCC 1 cut(s) 2804
BglI GCCNNNNNGGC 2 cut(s) 57, 248
Bme1390I CCNGG 6 cut(s) 172, 474, 905, 1395, 1419, 2232
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 2 cut(s) 89, 2773
BmgBI CACGTC 2 cut(s) 97, 180
BmgT120I GGNCC 3 cut(s) 19, 240, 989
BmiI GGNNCC 7 cut(s) 6, 20, 1558, 1744, 1772, 1981, 2181
BmrFI CCNGG 6 cut(s) 172, 474, 905, 1395, 1419, 2232
BmrI ACTGGG 5 cut(s) 357, 638, 1191, 1497, 2882
BmtI GCTAGC 2 cut(s) 804, 2079
BmuI ACTGGG 5 cut(s) 357, 638, 1191, 1497, 2882
BpiI GAAGAC 2 cut(s) 90, 312
BpmI CTGGAG 5 cut(s) 8, 1000, 1296, 1416, 1761
Bpu10I CCTNAGC 2 cut(s) 236, 357
Bpu14I TTCGAA 1 cut(s) 996
BpuMI CCSGG 1 cut(s) 172
BsaAI YACGTR 2 cut(s) 1567, 1960
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 3 cut(s) 43, 1197, 1619
BsaJI CCNNGG 8 cut(s) 52, 246, 473, 777, 1122, 1990, 2817, 2891
BsaWI WCCGGW 1 cut(s) 398
BsaXI ACNNNNNCTCC 2 cut(s) 1755, 1785
Bsc4I CCNNNNNNNGG 7 cut(s) 1439, 1781, 1996, 2176, 2237, 2329, 2873
Bse118I RCCGGY 2 cut(s) 2566, 2900
Bse1I ACTGG 8 cut(s) 363, 633, 1186, 1492, 2245, 2480, 2877, 2994
Bse21I CCTNAGG 1 cut(s) 2954
Bse3DI GCAATG 3 cut(s) 1065, 1830, 2628
BseBI CCWGG 5 cut(s) 474, 905, 1395, 1419, 2232
BseDI CCNNGG 8 cut(s) 52, 246, 473, 777, 1122, 1990, 2817, 2891
BseGI GGATG 5 cut(s) 150, 180, 1114, 2201, 2241
BseLI CCNNNNNNNGG 7 cut(s) 1439, 1781, 1996, 2176, 2237, 2329, 2873
BseMI GCAATG 3 cut(s) 1065, 1830, 2628
BseMII CTCAG 5 cut(s) 28, 250, 348, 925, 2226
BseNI ACTGG 8 cut(s) 363, 633, 1186, 1492, 2245, 2480, 2877, 2994
BseRI GAGGAG 2 cut(s) 1938, 2932
BseSI GKGCMC 3 cut(s) 1162, 1561, 1849
BseX3I CGGCCG 1 cut(s) 49
BseXI GCAGC 9 cut(s) 172, 394, 443, 1060, 1082, 2425, 2647, 2726, 2871
BseYI CCCAGC 1 cut(s) 332
BsgI GTGCAG 2 cut(s) 450, 1194
Bsh1236I CGCG 1 cut(s) 54
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 5 cut(s) 51, 242, 991, 1136, 2822
BshNI GGYRCC 3 cut(s) 4, 1556, 1979
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 6 cut(s) 214, 237, 1162, 1849, 1945, 2252
BsiHKCI CYCGRG 2 cut(s) 89, 2773
BsiSI CCGG 5 cut(s) 48, 171, 399, 2567, 2901
BslFI GGGAC 3 cut(s) 32, 2469, 2707
BslI CCNNNNNNNGG 7 cut(s) 1439, 1781, 1996, 2176, 2237, 2329, 2873
BsmAI GTCTC 7 cut(s) 17, 43, 60, 697, 1197, 1619, 2215
BsmBI CGTCTC 1 cut(s) 60
BsmFI GGGAC 3 cut(s) 32, 2469, 2707
BsnI GGCC 5 cut(s) 51, 242, 991, 1136, 2822
Bso31I GGTCTC 3 cut(s) 43, 1197, 1619
BsoBI CYCGRG 2 cut(s) 89, 2773
Bsp119I TTCGAA 1 cut(s) 996
Bsp1407I TGTACA 1 cut(s) 598
Bsp143I GATC 9 cut(s) 283, 529, 751, 773, 1586, 2035, 2754, 2767, 2877
Bsp19I CCATGG 2 cut(s) 2817, 2891
BspANI GGCC 5 cut(s) 51, 242, 991, 1136, 2822
BspCNI CTCAG 5 cut(s) 27, 249, 349, 924, 2225
BspFNI CGCG 1 cut(s) 54
BspLI GGNNCC 7 cut(s) 6, 20, 1558, 1744, 1772, 1981, 2181
BspMAI CTGCAG 1 cut(s) 2071
BspMI ACCTGC 1 cut(s) 25
BspOI GCTAGC 2 cut(s) 804, 2079
BspPI GGATC 7 cut(s) 278, 524, 746, 768, 1594, 2043, 2885
BspQI GCTCTTC 1 cut(s) 219
BspT104I TTCGAA 1 cut(s) 996
BspT107I GGYRCC 3 cut(s) 4, 1556, 1979
BspTI CTTAAG 1 cut(s) 2051
BspTNI GGTCTC 3 cut(s) 43, 1197, 1619
BsrBI CCGCTC 2 cut(s) 1631, 2555
BsrDI GCAATG 3 cut(s) 1065, 1830, 2628
BsrFI RCCGGY 2 cut(s) 2566, 2900
BsrGI TGTACA 1 cut(s) 598
BsrI ACTGG 8 cut(s) 363, 633, 1186, 1492, 2245, 2480, 2877, 2994
BssAI RCCGGY 2 cut(s) 2566, 2900
BssECI CCNNGG 8 cut(s) 52, 246, 473, 777, 1122, 1990, 2817, 2891
BssMI GATC 9 cut(s) 283, 529, 751, 773, 1586, 2035, 2754, 2767, 2877
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 5 cut(s) 777, 1122, 1990, 2817, 2891
Bst2UI CCWGG 5 cut(s) 474, 905, 1395, 1419, 2232
Bst4CI ACNGT 6 cut(s) 154, 538, 1597, 1696, 1767, 2005
Bst6I CTCTTC 1 cut(s) 219
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 2051
BstAPI GCANNNNNTGC 1 cut(s) 2247
BstAUI TGTACA 1 cut(s) 598
BstBAI YACGTR 2 cut(s) 1567, 1960
BstBI TTCGAA 1 cut(s) 996
BstDEI CTNAG 6 cut(s) 14, 236, 357, 911, 2212, 2954
BstDSI CCRYGG 4 cut(s) 52, 246, 2817, 2891
BstF5I GGATG 5 cut(s) 150, 180, 1114, 2201, 2241
BstFNI CGCG 1 cut(s) 54
BstH2I RGCGCY 4 cut(s) 8, 81, 1060, 1899
BstHHI GCGC 4 cut(s) 7, 80, 1059, 1898
BstKTI GATC 9 cut(s) 286, 532, 754, 776, 1589, 2038, 2757, 2770, 2880
BstMAI GTCTC 7 cut(s) 17, 43, 60, 697, 1197, 1619, 2215
BstMBI GATC 9 cut(s) 283, 529, 751, 773, 1586, 2035, 2754, 2767, 2877
BstMCI CGRYCG 1 cut(s) 52
BstNI CCWGG 5 cut(s) 474, 905, 1395, 1419, 2232
BstNSI RCATGY 3 cut(s) 429, 1839, 2910
BstSCI CCNGG 6 cut(s) 170, 472, 903, 1393, 1417, 2230
BstSFI CTRYAG 5 cut(s) 489, 1317, 1695, 2001, 2067
BstSLI GKGCMC 3 cut(s) 1162, 1561, 1849
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 9 cut(s) 172, 394, 443, 1060, 1082, 2425, 2647, 2726, 2871
BstV2I GAAGAC 2 cut(s) 90, 312
BstX2I RGATCY 2 cut(s) 773, 1586
BstXI CCANNNNNNTGG 2 cut(s) 1767, 2606
BstYI RGATCY 2 cut(s) 773, 1586
BstZI CGGCCG 1 cut(s) 49
Bsu36I CCTNAGG 1 cut(s) 2954
BsuI GTATCC 1 cut(s) 2804
BsuRI GGCC 5 cut(s) 51, 242, 991, 1136, 2822
BtgI CCRYGG 4 cut(s) 52, 246, 2817, 2891
BtrI CACGTC 2 cut(s) 97, 180
BtsCI GGATG 5 cut(s) 150, 180, 1114, 2201, 2241
BtsI GCAGTG 1 cut(s) 2086
BtsIMutI CAGTG 8 cut(s) 370, 655, 919, 1179, 1772, 2086, 2252, 2870
BveI ACCTGC 1 cut(s) 25
CaiI CAGNNNCTG 2 cut(s) 1181, 2009
CfoI GCGC 4 cut(s) 7, 80, 1059, 1898
Cfr10I RCCGGY 2 cut(s) 2566, 2900
Cfr13I GGNCC 3 cut(s) 19, 240, 989
Cfr42I CCGCGG 1 cut(s) 55
CseI GACGC 1 cut(s) 211
Csp6I GTAC 6 cut(s) 599, 1235, 1422, 1568, 1957, 2588
CviQI GTAC 6 cut(s) 599, 1235, 1422, 1568, 1957, 2588
DdeI CTNAG 6 cut(s) 14, 236, 357, 911, 2212, 2954
DinI GGCGCC 1 cut(s) 6
DpnI GATC 9 cut(s) 285, 531, 753, 775, 1588, 2037, 2756, 2769, 2879
DpnII GATC 9 cut(s) 283, 529, 751, 773, 1586, 2035, 2754, 2767, 2877
DrdI GACNNNNNNGTC 1 cut(s) 711
DseDI GACNNNNNNGTC 1 cut(s) 711
EaeI YGGCCR 1 cut(s) 49
EagI CGGCCG 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
EciI GGCGGA 1 cut(s) 78
Ecl136II GAGCTC 2 cut(s) 212, 1943
EclXI CGGCCG 1 cut(s) 49
Eco130I CCWWGG 5 cut(s) 777, 1122, 1990, 2817, 2891
Eco24I GRGCYC 5 cut(s) 214, 363, 1084, 1945, 2184
Eco31I GGTCTC 3 cut(s) 43, 1197, 1619
Eco32I GATATC 1 cut(s) 2190
Eco47I GGWCC 1 cut(s) 19
Eco47III AGCGCT 1 cut(s) 1058
Eco52I CGGCCG 1 cut(s) 49
Eco53kI GAGCTC 2 cut(s) 212, 1943
Eco57I CTGAAG 5 cut(s) 102, 200, 242, 291, 777
Eco81I CCTNAGG 1 cut(s) 2954
Eco88I CYCGRG 2 cut(s) 89, 2773
EcoICRI GAGCTC 2 cut(s) 212, 1943
EcoO109I RGGNCCY 2 cut(s) 19, 240
EcoRI GAATTC 1 cut(s) 121
EcoRII CCWGG 5 cut(s) 472, 903, 1393, 1417, 2230
EcoRV GATATC 1 cut(s) 2190
EcoT14I CCWWGG 5 cut(s) 777, 1122, 1990, 2817, 2891
EcoT22I ATGCAT 1 cut(s) 2912
EcoT38I GRGCYC 5 cut(s) 214, 363, 1084, 1945, 2184
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 5 cut(s) 777, 1122, 1990, 2817, 2891
Esp3I CGTCTC 1 cut(s) 60
FalI AAGNNNNNCTT 2 cut(s) 835, 867
FaqI GGGAC 3 cut(s) 32, 2469, 2707
FauNDI CATATG 1 cut(s) 2383
FbaI TGATCA 1 cut(s) 2754
FokI GGATG 5 cut(s) 157, 187, 1121, 2208, 2228
FriOI GRGCYC 5 cut(s) 214, 363, 1084, 1945, 2184
FspBI CTAG 6 cut(s) 512, 771, 801, 2076, 2093, 2657
GlaI GCGC 4 cut(s) 6, 79, 1058, 1897
GsaI CCCAGC 1 cut(s) 336
GsuI CTGGAG 5 cut(s) 8, 1000, 1296, 1416, 1761
HaeII RGCGCY 4 cut(s) 8, 81, 1060, 1899
HaeIII GGCC 5 cut(s) 51, 242, 991, 1136, 2822
HapII CCGG 5 cut(s) 48, 171, 399, 2567, 2901
HgaI GACGC 1 cut(s) 211
HhaI GCGC 4 cut(s) 7, 80, 1059, 1898
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 4 cut(s) 5, 78, 1057, 1896
HinP1I GCGC 4 cut(s) 5, 78, 1057, 1896
HincII GTYRAC 2 cut(s) 1519, 1716
HindII GTYRAC 2 cut(s) 1519, 1716
HinfI GANTC 6 cut(s) 10, 110, 262, 2307, 2637, 2645
HpaII CCGG 5 cut(s) 48, 171, 399, 2567, 2901
HphI GGTGA 5 cut(s) 712, 1016, 1039, 1540, 2642
Hpy166II GTNNAC 6 cut(s) 442, 1160, 1519, 1716, 1847, 2084
Hpy8I GTNNAC 6 cut(s) 442, 1160, 1519, 1716, 1847, 2084
HpyAV CCTTC 7 cut(s) 134, 446, 668, 1571, 2154, 2381, 2877
HpyCH4III ACNGT 6 cut(s) 154, 538, 1597, 1696, 1767, 2005
HpyCH4IV ACGT 7 cut(s) 96, 179, 1403, 1523, 1566, 1959, 2590
HpyF3I CTNAG 6 cut(s) 14, 236, 357, 911, 2212, 2954
HpySE526I ACGT 7 cut(s) 96, 179, 1403, 1523, 1566, 1959, 2590
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 4 cut(s) 5, 78, 1057, 1896
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 1 cut(s) 2566
KroNI GCCGGC 1 cut(s) 2568
Ksp22I TGATCA 1 cut(s) 2754
KspI CCGCGG 1 cut(s) 55
Kzo9I GATC 9 cut(s) 283, 529, 751, 773, 1586, 2035, 2754, 2767, 2877
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 8 cut(s) 209, 1651, 1742, 1751, 1776, 2179, 2945, 2955
Lsp1109I GCAGC 9 cut(s) 172, 394, 443, 1060, 1082, 2425, 2647, 2726, 2871
MaeI CTAG 6 cut(s) 512, 771, 801, 2076, 2093, 2657
MaeII ACGT 7 cut(s) 96, 179, 1403, 1523, 1566, 1959, 2590
MaeIII GTNAC 7 cut(s) 166, 763, 1177, 1483, 1573, 2005, 2488
MalI GATC 9 cut(s) 285, 531, 753, 775, 1588, 2037, 2756, 2769, 2879
MbiI CCGCTC 2 cut(s) 1631, 2555
MboI GATC 9 cut(s) 283, 529, 751, 773, 1586, 2035, 2754, 2767, 2877
MboII GAAGA 9 cut(s) 95, 149, 206, 317, 338, 584, 1406, 2363, 2759
MfeI CAATTG 2 cut(s) 321, 2706
MflI RGATCY 2 cut(s) 773, 1586
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 19, 2639
MmeI TCCRAC 4 cut(s) 66, 1339, 1564, 2342
Mph1103I ATGCAT 1 cut(s) 2912
MroNI GCCGGC 1 cut(s) 2566
MroXI GAANNNNTTC 2 cut(s) 456, 1093
MseI TTAA 6 cut(s) 1335, 1406, 1614, 1953, 2052, 2541
MslI CAYNNNNRTG 3 cut(s) 1526, 2030, 2915
MspA1I CMGCKG 4 cut(s) 54, 1073, 1871, 2717
MspCI CTTAAG 1 cut(s) 2051
MspI CCGG 5 cut(s) 48, 171, 399, 2567, 2901
MspR9I CCNGG 6 cut(s) 172, 474, 905, 1395, 1419, 2232
MunI CAATTG 2 cut(s) 321, 2706
MvaI CCWGG 5 cut(s) 474, 905, 1395, 1419, 2232
MvnI CGCG 1 cut(s) 54
NaeI GCCGGC 1 cut(s) 2568
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 172
NcoI CCATGG 2 cut(s) 2817, 2891
NdeI CATATG 1 cut(s) 2383
NdeII GATC 9 cut(s) 283, 529, 751, 773, 1586, 2035, 2754, 2767, 2877
NgoMIV GCCGGC 1 cut(s) 2566
NheI GCTAGC 2 cut(s) 800, 2075
NlaIV GGNNCC 7 cut(s) 6, 20, 1558, 1744, 1772, 1981, 2181
NmeAIII GCCGAG 1 cut(s) 33
NmuCI GTSAC 2 cut(s) 763, 1177
NsiI ATGCAT 1 cut(s) 2912
NspI RCATGY 3 cut(s) 429, 1839, 2910
NspV TTCGAA 1 cut(s) 996
OliI CACNNNNGTG 1 cut(s) 1526
PaeI GCATGC 2 cut(s) 1839, 2910
PaeR7I CTCGAG 2 cut(s) 89, 2773
PciI ACATGT 1 cut(s) 425
PciSI GCTCTTC 1 cut(s) 219
PdiI GCCGGC 1 cut(s) 2568
PdmI GAANNNNTTC 2 cut(s) 456, 1093
PfeI GAWTC 4 cut(s) 110, 262, 2307, 2637
PflFI GACNNNGTC 1 cut(s) 557
PleI GAGTC 2 cut(s) 18, 2639
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 18, 2639
Ppu21I YACGTR 2 cut(s) 1567, 1960
PpuMI RGGWCCY 1 cut(s) 19
PscI ACATGT 1 cut(s) 425
PshBI ATTAAT 1 cut(s) 1614
Psp124BI GAGCTC 2 cut(s) 214, 1945
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 5 cut(s) 472, 903, 1393, 1417, 2230
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 5 cut(s) 472, 903, 1393, 1417, 2230
PspN4I GGNNCC 7 cut(s) 6, 20, 1558, 1744, 1772, 1981, 2181
PspPI GGNCC 3 cut(s) 19, 240, 989
PspPPI RGGWCCY 1 cut(s) 19
PspXI VCTCGAGB 1 cut(s) 89
PstI CTGCAG 1 cut(s) 2071
PstNI CAGNNNCTG 2 cut(s) 1181, 2009
PsuI RGATCY 2 cut(s) 773, 1586
PsyI GACNNNGTC 1 cut(s) 557
PvuII CAGCTG 3 cut(s) 1073, 1871, 2717
RsaI GTAC 6 cut(s) 600, 1236, 1423, 1569, 1958, 2589
RsaNI GTAC 6 cut(s) 599, 1235, 1422, 1568, 1957, 2588
RseI CAYNNNNRTG 3 cut(s) 1526, 2030, 2915
SacI GAGCTC 2 cut(s) 214, 1945
SacII CCGCGG 1 cut(s) 55
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 6 cut(s) 1335, 1406, 1614, 1953, 2052, 2541
Sau3AI GATC 9 cut(s) 283, 529, 751, 773, 1586, 2035, 2754, 2767, 2877
Sau96I GGNCC 3 cut(s) 19, 240, 989
SchI GAGTC 2 cut(s) 19, 2639
ScrFI CCNGG 6 cut(s) 172, 474, 905, 1395, 1419, 2232
SfcI CTRYAG 5 cut(s) 489, 1317, 1695, 2001, 2067
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 2 cut(s) 89, 2773
Sfr303I CCGCGG 1 cut(s) 55
SfuI TTCGAA 1 cut(s) 996
SgrBI CCGCGG 1 cut(s) 55
SinI GGWCC 1 cut(s) 19
SlaI CTCGAG 2 cut(s) 89, 2773
SmiMI CAYNNNNRTG 3 cut(s) 1526, 2030, 2915
SmlI CTYRAG 3 cut(s) 89, 2051, 2773
SmoI CTYRAG 3 cut(s) 89, 2051, 2773
SphI GCATGC 2 cut(s) 1839, 2910
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 6 cut(s) 512, 771, 801, 2076, 2093, 2657
SstI GAGCTC 2 cut(s) 214, 1945
StyD4I CCNGG 6 cut(s) 170, 472, 903, 1393, 1417, 2230
StyI CCWWGG 5 cut(s) 777, 1122, 1990, 2817, 2891
TaaI ACNGT 6 cut(s) 154, 538, 1597, 1696, 1767, 2005
TaiI ACGT 7 cut(s) 99, 182, 1406, 1526, 1569, 1962, 2593
TaqI TCGA 7 cut(s) 90, 410, 934, 996, 1623, 2310, 2774
TatI WGTACW 2 cut(s) 598, 1234
TauI GCSGC 8 cut(s) 54, 57, 60, 63, 75, 78, 2852, 2890
TfiI GAWTC 4 cut(s) 110, 262, 2307, 2637
Tru1I TTAA 6 cut(s) 1335, 1406, 1614, 1953, 2052, 2541
Tru9I TTAA 6 cut(s) 1335, 1406, 1614, 1953, 2052, 2541
TscAI CASTG 8 cut(s) 370, 655, 919, 1186, 1772, 2086, 2252, 2877
TseFI GTSAC 2 cut(s) 763, 1177
TseI GCWGC 9 cut(s) 185, 382, 431, 1070, 1073, 2438, 2660, 2714, 2884
Tsp45I GTSAC 2 cut(s) 763, 1177
TspDTI ATGAA 5 cut(s) 882, 883, 957, 2400, 2583
TspGWI ACGGA 2 cut(s) 235, 387
TspRI CASTG 8 cut(s) 370, 655, 919, 1186, 1772, 2086, 2252, 2877
Tth111I GACNNNGTC 1 cut(s) 557
Vha464I CTTAAG 1 cut(s) 2051
VneI GTGCAC 2 cut(s) 1158, 1845
VpaK11BI GGWCC 1 cut(s) 19
VspI ATTAAT 1 cut(s) 1614
XapI RAATTY 3 cut(s) 121, 1546, 1658
XbaI TCTAGA 1 cut(s) 770
XceI RCATGY 3 cut(s) 429, 1839, 2910
XcmI CCANNNNNNNNNTGG 1 cut(s) 1822
XhoI CTCGAG 2 cut(s) 89, 2773
XmnI GAANNNNTTC 2 cut(s) 456, 1093
XspI CTAG 6 cut(s) 512, 771, 801, 2076, 2093, 2657
Zsp2I ATGCAT 1 cut(s) 2912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.