Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0009249

Physical Location & Seq

Genomic Coordinates Forward (+)

7594410 .. 7600867

Gene Structure 6458 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g12710.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3000 bp

ATGGCGCCGAGTCTCAGGGACCTCCAGCTCACGCAGCTCTCCAAGTCTTCCGCCGGCGACGGCGACGACGGAGACCACGAGGGCGTGAGGCTCTTGGATTCGTATGAGAAATCCGGGGAGGGAGTTGAGGAGGAGGGGACGAGGAGGGTGCAGGTTCGAGTCACTGGGATGACGTGTGCTGCTTGCTCTAATTCCGTCGAAGGCGCTCTCCGGAGCGTCAATGGCGTGCTCACCGCCTCCGTCGCGTTGCTTCAGAACCGCGCCGACGTCGTTTTTGATCTCCGATTGGTCAAGGATGAAGACATAAAAAATGCAATTGAAGATGCTGGGTTTGAGGCAGAGGTTATACCTGATCCCAGTACAAATGGAGTGAAGCAGCAGGGGACCCTGACGGGTCAGTTTTCGATAGGAGGTATGACATGTGCAGCTTGTGTGAACTCTGTTGAAGGTATTCTGAAAGGGCTTCCTGGGGTCAAAAGAGCTGTAGTAGCTTTAGCTACTTCACTAGGAGAAGTGGAGTATGATCCTACTGTAATTAGTAAAGATGACATAGTGAATGCCATTGAAGATGCTGGTTTTGAAGGATCGCTTGTACAGAGTAGTCAGCAGGATAAAATTATACTGGGAGTTGCTGGCATGTTCAATGAGATAGATGCACAGGTTTTAGAAGCCATAATCTGCAACTTAAAAGGGGTTAGACATTTTCGTCTCGACAGGATTTCAAGAGAACTTGAAATTCTCTTTGATCCTGAAGTTGTCACGTCTAGATCCTTGGTTGATGGGATTGAGGGGGCTAGCAATGGGAAGTTCAAACTCCAAGTAGCAAACCCTTATACAAGAATGACCTGCAAAGATGCTGATGAAGCCGCAAATATGTTTCGGCTTTTCATCTCCAGCCTTGTTCTCAGTGTTCCTGTCTTTCTCATTCGAGTAGTTTGTCCTCACATTCCACTTCTGTATTCATTATTGCTCTGGAGATGTGGCCCCTTTGAAATGGGTGATTGGTTGAAGTGGGCATTGGTGAGTGTTGTTCAATTTGTTATTGGGAAGCGCTTCTACATTGCAGCTGCAAGAGCTCTACGAAATGGTTCCACTAACATGGATGTTCTGGTTGCCCTGGGAACTTCGGCCTCTTACTTTTACTCTGTTTGTGCACTCCTATATGGTGCAGTCACTGGATTTTGGTCTCCCACATACTTTGAAACAAGTGCCATGCTAATAACATTCGTACTGTTGGGTAAGTATCTGGAATGTCTTGCAAAAGGAAAGACATCAGATGCTATCAAGAAGTTGATAGAACTTGCTCCAGCTACAGCTTTATTGCTTGTCAAAGATAAAGGTGGGAGATATGTTGGGGAGAGGGAAATTGATGCTCTGTTAATTCAGCCTGGAGATACATTAAAAGTTCTTCCTGGTACAAAAGTTCCTGCTGATGGTATGGTTGTGTGGGGCTCAAGTTACGTAAATGAGAGTATGGTAACTGGTGAAGCTATACCTGTTTTAAAGGAAGTCAACTCATTGGTTATTGGAGGAACAATAAATTTACATGGTGCGCTTCACATTCAAGTTACAAAAGTCGGATCTGATACAGTTTTACATCAGATAATTAATTTGGTGGAGACTGCTCAAATGTCCAAAGCTCCTATTCAGAAATTTGCTGATTTTGTTGCAAGCATTTTTGTTCCTACTGTAGTTGCATTGTCATTGCTGACGTTTTTGGGCTGGTATGCCGCTGGAGCCTTTGGAGCTTACCCAGAACAGTGGCTCCCCGAAAATGGAAATCACTTTGTTTTTGCCCTTATGTTTTCCATATCAGTTGTGGTCATTGCATGCCCCTGTGCACTTGGCTTGGCAACACCAACTGCTGTCATGGTTGCGACAGGGGTTGGTGCTAATAATGGTGTGCTGATAAAAGGAGGAGATGCTCTGGAAAGAGCTCAAAAGATTAATTACGTCATATTTGATAAAACAGGCACTCTAACCCAAGGAAAGGCTACAGTTACTGCTGTAAAAGTTTTCACAGGAATGGATCGTGGAGATTTTCTCAAATTGGTGGCTTCTGCAGAGGCTAGCAGTGAACACCCACTGGGGAAAGCAATAGTTGAATATGCACGCCATTTCCACTTCTTTGATGAACCTTCTGCAACCAATGCAACAAATCAAAGCAAGGAGCCCGTGATCTCTGAATGGCTTTTCGATGTCTCAGATTTCTTTGCTTTGCCTGGTAGAGGCATCCAGTGCTTAATAGATGGAAAACTTATTCTGGTTGGTAATAGAAAATTGATGACTGAAAGTGGAATCGACATTCCAACTGATGTTGAGAACTTTGTAGTAGAGCTGGAAGAAAGTGCAAAGACAGGCATACTCGTCGCATATGAAGGCAATTTGGTTGGAGTATTGGGGGTTGCTGACCCACTAAAAAGAGAGGCTGCCATAGTCATAGAGGGACTTGTTAAGATGGGTGTCAGACCAGTCATGGTTACAGGAGATAACTGGAGGACAGCACAAGCTGTTGCTAAGGAGGTTGGCATTAAAGATGTGCGAGCAGAGGTAATGCCGGCTGGAAAAGCAGATGTCGTACGCTCATTCCAAAAAGATGGAAGCATAGTTGCAATGGTTGGTGATGGAATCAATGACTCTCCTGCCCTAGCCGCTTCTGATGTCGGTATGGCAATAGGGGCAGGGACAGATATTGCAATTGAAGCAGCTCACTATGTATTGATGAGAAATAACTTGGAAGATGTGATCACAGCCATTGATCTCTCAAGAAAGACCTTCACTCGTATTCGATTGAATTATGTGTTTGCCATGGCCTACAATGTCATCGCAATACCTATTGCGGCAGGGGTTTTCTTTCCCTCACTGGGGATCATGTTGCCGCCATGGGTAGCCGGTGCATGCATGGCTATGTCATCTGTTAGTGTTGTATGCTCCTCTTTGCTCCTAAGAAGATACAGAAAACCCCGACTTACCGCTATACTGGAAATAGTTGTAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0012505 GO:0016020 GO:0023052 GO:0031410 GO:0031982 GO:0031984 GO:0032870 GO:0035556 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071369 GO:0071495 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1000

Amino Acids

107.02

Weight (kDa)

5.22

Isoelectric Point (pI)

30.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	52 - 112	2.1e-12	Heavy-metal-associated domain
HMA	PF00403	134 - 194	1.8e-15	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	436 - 540	8.2e-24	P-type ATPase actuator domain
Hydrolase	PF00702	650 - 841	8.6e-17	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44790
fragaria_vesca	FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710
malus_domestica	MD05G1247900.v1.1 MD10G1228800.v1.1
prunus_persica	Prupe.4G115900_v2.0.a1 Prupe.4G115900_v2.0.a1
pyrus_communis	pycom05g22420 pycom10g19240
rosa_chinensis	RchiOBHm_Chr5g0020701
rosa_laevigata	RLG00000032554
rosa_multiflora	Rmu_sc0005607.1_g000032
rosa_roxburghii	Rroxscaffold_1G00057600
rosa_rugosa	Rorug05G0062500
rosa_samantha	Rh5AG152200 Rh5BG151900 Rh5CG163300 Rh5DG151400
rosa_wichuraiana	Rw5G013490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	705
AatII	GACGTC	1 cut(s)	270
Acc36I	ACCTGC	2 cut(s)	142, 854
AccB1I	GGYRCC	1 cut(s)	4
AccII	CGCG	2 cut(s)	245, 261
AccIII	TCCGGA	1 cut(s)	210
AciI	CCGC	9 cut(s)	51, 234, 259, 867, 1731, 2652, 2840, 2879, 2973
AclWI	GGATC	8 cut(s)	347, 518, 592, 740, 762, 1588, 2037, 2876
AcsI	RAATTY	3 cut(s)	735, 1540, 1652
AcuI	CTGAAG	2 cut(s)	236, 771
AcyI	GRCGYC	2 cut(s)	5, 267
AfaI	GTAC	5 cut(s)	361, 594, 1230, 1417, 2580
AfeI	AGCGCT	1 cut(s)	1052
AfiI	CCNNNNNNNGG	6 cut(s)	1433, 1775, 1990, 2228, 2864, 2865
AflIII	ACRYGT	2 cut(s)	173, 419
AjiI	CACGTC	2 cut(s)	174, 762
AjnI	CCWGG	5 cut(s)	466, 1116, 1387, 1411, 2221
AjuI	GAANNNNNNNTTGG	2 cut(s)	1001, 1033
AloI	GAACNNNNNNTCC	2 cut(s)	1749, 1781
Alw21I	GWGCWC	5 cut(s)	231, 1078, 1156, 1843, 1939
Alw26I	GTCTC	6 cut(s)	17, 66, 713, 1191, 1613, 2206
Alw44I	GTGCAC	2 cut(s)	1152, 1839
AlwI	GGATC	8 cut(s)	347, 518, 592, 740, 762, 1588, 2037, 2876
AlwNI	CAGNNNCTG	2 cut(s)	1175, 2003
Aor13HI	TCCGGA	1 cut(s)	210
Aor51HI	AGCGCT	1 cut(s)	1052
AoxI	GGCC	3 cut(s)	982, 1128, 2811
ApaLI	GTGCAC	2 cut(s)	1152, 1839
ApeKI	GCWGC	8 cut(s)	34, 179, 376, 425, 1064, 1067, 2429, 2705
ApoI	RAATTY	3 cut(s)	735, 1540, 1652
ArsI	GACNNNNNNTTYG	2 cut(s)	257, 289
AseI	ATTAAT	2 cut(s)	1608, 1947
Asp700I	GAANNNNTTC	3 cut(s)	450, 1052, 1087
AspLEI	GCGC	5 cut(s)	7, 206, 263, 1053, 1555
AspS9I	GGNCC	3 cut(s)	19, 384, 983
AsuC2I	CCSGG	1 cut(s)	115
AsuHPI	GGTGA	5 cut(s)	223, 1010, 1033, 1496, 2633
AsuNHI	GCTAGC	2 cut(s)	794, 2069
AvaII	GGWCC	2 cut(s)	19, 384
BaeGI	GKGCMC	2 cut(s)	1156, 1843
BaeI	ACNNNNGTAYC	2 cut(s)	1407, 1440
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	4 cut(s)	1078, 1454, 1939, 2175
BarI	GAAGNNNNNNTAC	2 cut(s)	1040, 1072
BauI	CACGAG	1 cut(s)	77
BbsI	GAAGAC	2 cut(s)	39, 306
Bbv12I	GWGCWC	5 cut(s)	231, 1078, 1156, 1843, 1939
BbvI	GCAGC	8 cut(s)	46, 166, 388, 437, 1054, 1076, 2416, 2717
BccI	CCATC	6 cut(s)	773, 1427, 2243, 2452, 2591, 2618
BceAI	ACGGC	1 cut(s)	76
BcgI	CGANNNNNNTGC	4 cut(s)	130, 164, 2350, 2384
BciT130I	CCWGG	5 cut(s)	468, 1118, 1389, 1413, 2223
BclI	TGATCA	1 cut(s)	2745
BcnI	CCSGG	1 cut(s)	115
BcoDI	GTCTC	6 cut(s)	17, 66, 713, 1191, 1613, 2206
BfaI	CTAG	5 cut(s)	506, 765, 795, 2070, 2648
BfmI	CTRYAG	5 cut(s)	483, 1311, 1689, 1995, 2061
BfoI	RGCGCY	3 cut(s)	8, 207, 1054
BfuAI	ACCTGC	2 cut(s)	142, 854
Bme1390I	CCNGG	6 cut(s)	115, 468, 1118, 1389, 1413, 2223
Bme18I	GGWCC	2 cut(s)	19, 384
BmgBI	CACGTC	2 cut(s)	174, 762
BmgT120I	GGNCC	3 cut(s)	19, 384, 983
BmiI	GGNNCC	9 cut(s)	6, 20, 385, 386, 985, 1090, 1738, 1766, 2172
BmrFI	CCNGG	6 cut(s)	115, 468, 1118, 1389, 1413, 2223
BmrI	ACTGGG	5 cut(s)	174, 351, 632, 2096, 2873
BmsI	GCATC	8 cut(s)	313, 559, 643, 844, 1267, 1360, 1912, 2241
BmtI	GCTAGC	2 cut(s)	798, 2073
BmuI	ACTGGG	5 cut(s)	174, 351, 632, 2096, 2873
BpiI	GAAGAC	2 cut(s)	39, 306
BplI	GAGNNNNNCTC	2 cut(s)	2028, 2060
BpmI	CTGGAG	7 cut(s)	8, 877, 994, 1290, 1410, 1755, 2515
Bpu10I	CCTNAGC	1 cut(s)	2517
BpuEI	CTTGAG	2 cut(s)	1438, 2749
BpuMI	CCSGG	1 cut(s)	115
BsaAI	YACGTR	1 cut(s)	1462
BsaHI	GRCGYC	2 cut(s)	5, 267
BsaI	GGTCTC	2 cut(s)	66, 1191
BsaJI	CCNNGG	8 cut(s)	114, 467, 771, 1116, 1117, 1984, 2808, 2882
BsaWI	WCCGGW	1 cut(s)	210
BsaXI	ACNNNNNCTCC	2 cut(s)	1749, 1779
Bsc4I	CCNNNNNNNGG	6 cut(s)	1433, 1775, 1990, 2228, 2864, 2865
Bse118I	RCCGGY	3 cut(s)	53, 2557, 2891
Bse3DI	GCAATG	5 cut(s)	805, 1059, 1703, 1824, 2619
BseAI	TCCGGA	1 cut(s)	210
BseBI	CCWGG	5 cut(s)	468, 1118, 1389, 1413, 2223
BseDI	CCNNGG	8 cut(s)	114, 467, 771, 1116, 1117, 1984, 2808, 2882
BseGI	GGATG	4 cut(s)	174, 301, 1108, 2232
BseLI	CCNNNNNNNGG	6 cut(s)	1433, 1775, 1990, 2228, 2864, 2865
BseMI	GCAATG	5 cut(s)	805, 1059, 1703, 1824, 2619
BseMII	CTCAG	3 cut(s)	28, 919, 2217
BseRI	GAGGAG	5 cut(s)	143, 146, 157, 1932, 2923
BseSI	GKGCMC	2 cut(s)	1156, 1843
BseXI	GCAGC	8 cut(s)	46, 166, 388, 437, 1054, 1076, 2416, 2717
BseYI	CCCAGC	1 cut(s)	326
BsgI	GTGCAG	3 cut(s)	170, 444, 1188
Bsh1236I	CGCG	2 cut(s)	245, 261
BshFI	GGCC	3 cut(s)	984, 1130, 2813
BshNI	GGYRCC	1 cut(s)	4
BsiHKAI	GWGCWC	5 cut(s)	231, 1078, 1156, 1843, 1939
BsiSI	CCGG	5 cut(s)	54, 114, 211, 2558, 2892
BsiWI	CGTACG	1 cut(s)	2578
BslFI	GGGAC	5 cut(s)	32, 151, 397, 2460, 2698
BslI	CCNNNNNNNGG	6 cut(s)	1433, 1775, 1990, 2228, 2864, 2865
BsmAI	GTCTC	6 cut(s)	17, 66, 713, 1191, 1613, 2206
BsmBI	CGTCTC	1 cut(s)	713
BsmFI	GGGAC	5 cut(s)	32, 151, 397, 2460, 2698
BsmI	GAATGC	1 cut(s)	562
BsnI	GGCC	3 cut(s)	984, 1130, 2813
Bso31I	GGTCTC	2 cut(s)	66, 1191
Bsp1286I	GDGCHC	7 cut(s)	231, 1078, 1156, 1454, 1843, 1939, 2175
Bsp13I	TCCGGA	1 cut(s)	210
Bsp1407I	TGTACA	1 cut(s)	592
Bsp19I	CCATGG	2 cut(s)	2808, 2882
BspACI	CCGC	9 cut(s)	51, 234, 259, 867, 1731, 2652, 2840, 2879, 2973
BspANI	GGCC	3 cut(s)	984, 1130, 2813
BspCNI	CTCAG	3 cut(s)	27, 918, 2216
BspEI	TCCGGA	1 cut(s)	210
BspFNI	CGCG	2 cut(s)	245, 261
BspLI	GGNNCC	9 cut(s)	6, 20, 385, 386, 985, 1090, 1738, 1766, 2172
BspMAI	CTGCAG	1 cut(s)	2065
BspMI	ACCTGC	2 cut(s)	142, 854
BspOI	GCTAGC	2 cut(s)	798, 2073
BspPI	GGATC	8 cut(s)	347, 518, 592, 740, 762, 1588, 2037, 2876
BspT107I	GGYRCC	1 cut(s)	4
BspTNI	GGTCTC	2 cut(s)	66, 1191
BsrDI	GCAATG	5 cut(s)	805, 1059, 1703, 1824, 2619
BsrFI	RCCGGY	3 cut(s)	53, 2557, 2891
BsrGI	TGTACA	1 cut(s)	592
BssAI	RCCGGY	3 cut(s)	53, 2557, 2891
BssECI	CCNNGG	8 cut(s)	114, 467, 771, 1116, 1117, 1984, 2808, 2882
BssNI	GRCGYC	2 cut(s)	5, 267
BssSI	CACGAG	1 cut(s)	77
BssT1I	CCWWGG	4 cut(s)	771, 1984, 2808, 2882
Bst2BI	CACGAG	1 cut(s)	77
Bst2UI	CCWGG	5 cut(s)	468, 1118, 1389, 1413, 2223
Bst4CI	ACNGT	6 cut(s)	532, 1233, 1591, 1690, 1761, 1999
BstACI	GRCGYC	2 cut(s)	5, 267
BstAPI	GCANNNNNTGC	2 cut(s)	2150, 2238
BstAUI	TGTACA	1 cut(s)	592
BstBAI	YACGTR	1 cut(s)	1462
BstDEI	CTNAG	5 cut(s)	14, 905, 2203, 2517, 2945
BstDSI	CCRYGG	2 cut(s)	2808, 2882
BstENI	CCTNNNNNAGG	1 cut(s)	2226
BstF5I	GGATG	4 cut(s)	174, 301, 1108, 2232
BstFNI	CGCG	2 cut(s)	245, 261
BstH2I	RGCGCY	3 cut(s)	8, 207, 1054
BstHHI	GCGC	5 cut(s)	7, 206, 263, 1053, 1555
BstMAI	GTCTC	6 cut(s)	17, 66, 713, 1191, 1613, 2206
BstNI	CCWGG	5 cut(s)	468, 1118, 1389, 1413, 2223
BstNSI	RCATGY	4 cut(s)	423, 640, 1833, 2901
BstSCI	CCNGG	6 cut(s)	113, 466, 1116, 1387, 1411, 2221
BstSFI	CTRYAG	5 cut(s)	483, 1311, 1689, 1995, 2061
BstSLI	GKGCMC	2 cut(s)	1156, 1843
BstSNI	TACGTA	1 cut(s)	1462
BstUI	CGCG	2 cut(s)	245, 261
BstV1I	GCAGC	8 cut(s)	46, 166, 388, 437, 1054, 1076, 2416, 2717
BstV2I	GAAGAC	2 cut(s)	39, 306
BstX2I	RGATCY	2 cut(s)	767, 1580
BstXI	CCANNNNNNTGG	3 cut(s)	1099, 1761, 2597
BstYI	RGATCY	2 cut(s)	767, 1580
BsuRI	GGCC	3 cut(s)	984, 1130, 2813
BtgI	CCRYGG	2 cut(s)	2808, 2882
BtgZI	GCGATG	1 cut(s)	2809
BtrI	CACGTC	2 cut(s)	174, 762
BtsCI	GGATG	4 cut(s)	174, 301, 1108, 2232
BtsI	GCAGTG	1 cut(s)	2080
BtsIMutI	CAGTG	8 cut(s)	162, 913, 1173, 1766, 2080, 2084, 2243, 2861
BveI	ACCTGC	2 cut(s)	142, 854
CaiI	CAGNNNCTG	2 cut(s)	1175, 2003
CfoI	GCGC	5 cut(s)	7, 206, 263, 1053, 1555
Cfr10I	RCCGGY	3 cut(s)	53, 2557, 2891
Cfr13I	GGNCC	3 cut(s)	19, 384, 983
CseI	GACGC	1 cut(s)	205
Csp6I	GTAC	5 cut(s)	360, 593, 1229, 1416, 2579
CspCI	CAANNNNNGTGG	2 cut(s)	1180, 1215
CviQI	GTAC	5 cut(s)	360, 593, 1229, 1416, 2579
DdeI	CTNAG	5 cut(s)	14, 905, 2203, 2517, 2945
DinI	GGCGCC	1 cut(s)	6
DraI	TTTAAA	1 cut(s)	1503
DrdI	GACNNNNNNGTC	1 cut(s)	705
DseDI	GACNNNNNNGTC	1 cut(s)	705
EciI	GGCGGA	1 cut(s)	40
Ecl136II	GAGCTC	2 cut(s)	1076, 1937
Eco105I	TACGTA	1 cut(s)	1462
Eco130I	CCWWGG	4 cut(s)	771, 1984, 2808, 2882
Eco24I	GRGCYC	4 cut(s)	1078, 1454, 1939, 2175
Eco31I	GGTCTC	2 cut(s)	66, 1191
Eco47I	GGWCC	2 cut(s)	19, 384
Eco47III	AGCGCT	1 cut(s)	1052
Eco53kI	GAGCTC	2 cut(s)	1076, 1937
Eco57I	CTGAAG	2 cut(s)	236, 771
EcoICRI	GAGCTC	2 cut(s)	1076, 1937
EcoNI	CCTNNNNNAGG	1 cut(s)	2226
EcoO109I	RGGNCCY	2 cut(s)	19, 384
EcoRII	CCWGG	5 cut(s)	466, 1116, 1387, 1411, 2221
EcoT14I	CCWWGG	4 cut(s)	771, 1984, 2808, 2882
EcoT22I	ATGCAT	1 cut(s)	2903
EcoT38I	GRGCYC	4 cut(s)	1078, 1454, 1939, 2175
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	4 cut(s)	771, 1984, 2808, 2882
Esp3I	CGTCTC	1 cut(s)	713
FalI	AAGNNNNNCTT	2 cut(s)	573, 605
FaqI	GGGAC	5 cut(s)	32, 151, 397, 2460, 2698
FauNDI	CATATG	1 cut(s)	2374
FbaI	TGATCA	1 cut(s)	2745
FokI	GGATG	4 cut(s)	181, 308, 1115, 2219
FriOI	GRGCYC	4 cut(s)	1078, 1454, 1939, 2175
FspBI	CTAG	5 cut(s)	506, 765, 795, 2070, 2648
GlaI	GCGC	5 cut(s)	6, 205, 262, 1052, 1554
GsaI	CCCAGC	1 cut(s)	330
GsuI	CTGGAG	7 cut(s)	8, 877, 994, 1290, 1410, 1755, 2515
HaeII	RGCGCY	3 cut(s)	8, 207, 1054
HaeIII	GGCC	3 cut(s)	984, 1130, 2813
HapII	CCGG	5 cut(s)	54, 114, 211, 2558, 2892
HgaI	GACGC	1 cut(s)	205
HhaI	GCGC	5 cut(s)	7, 206, 263, 1053, 1555
Hin1I	GRCGYC	2 cut(s)	5, 267
Hin6I	GCGC	5 cut(s)	5, 204, 261, 1051, 1553
HinP1I	GCGC	5 cut(s)	5, 204, 261, 1051, 1553
HincII	GTYRAC	1 cut(s)	1513
HindII	GTYRAC	1 cut(s)	1513
HinfI	GANTC	6 cut(s)	10, 98, 159, 2298, 2628, 2636
HpaII	CCGG	5 cut(s)	54, 114, 211, 2558, 2892
HphI	GGTGA	5 cut(s)	223, 1010, 1033, 1496, 2633
Hpy166II	GTNNAC	5 cut(s)	436, 1154, 1513, 1841, 2078
Hpy8I	GTNNAC	5 cut(s)	436, 1154, 1513, 1841, 2078
Hpy99I	CGWCG	8 cut(s)	62, 68, 71, 200, 245, 269, 272, 2372
HpyAV	CCTTC	6 cut(s)	194, 440, 575, 2148, 2372, 2785
HpyCH4III	ACNGT	6 cut(s)	532, 1233, 1591, 1690, 1761, 1999
HpyCH4IV	ACGT	6 cut(s)	173, 267, 761, 1461, 1712, 1953
HpyF3I	CTNAG	5 cut(s)	14, 905, 2203, 2517, 2945
HpySE526I	ACGT	6 cut(s)	173, 267, 761, 1461, 1712, 1953
Hsp92I	GRCGYC	2 cut(s)	5, 267
HspAI	GCGC	5 cut(s)	5, 204, 261, 1051, 1553
KasI	GGCGCC	1 cut(s)	4
KflI	GGGWCCC	1 cut(s)	384
Kpn2I	TCCGGA	1 cut(s)	210
KroI	GCCGGC	2 cut(s)	53, 2557
KroNI	GCCGGC	2 cut(s)	55, 2559
Ksp22I	TGATCA	1 cut(s)	2745
LmnI	GCTCC	9 cut(s)	213, 1309, 1645, 1736, 1745, 1770, 2170, 2936, 2946
Lsp1109I	GCAGC	8 cut(s)	46, 166, 388, 437, 1054, 1076, 2416, 2717
LweI	GCATC	8 cut(s)	313, 559, 643, 844, 1267, 1360, 1912, 2241
MaeI	CTAG	5 cut(s)	506, 765, 795, 2070, 2648
MaeII	ACGT	6 cut(s)	173, 267, 761, 1461, 1712, 1953
MaeIII	GTNAC	8 cut(s)	160, 757, 1171, 1457, 1477, 1567, 1999, 2479
MboII	GAAGA	8 cut(s)	39, 311, 332, 578, 1400, 2354, 2750, 2961
MfeI	CAATTG	2 cut(s)	315, 2697
MflI	RGATCY	2 cut(s)	767, 1580
MhlI	GDGCHC	7 cut(s)	231, 1078, 1156, 1454, 1843, 1939, 2175
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	19, 168, 2630
MmeI	TCCRAC	3 cut(s)	1558, 2333, 2371
Mph1103I	ATGCAT	1 cut(s)	2903
MreI	CGCCGGCG	1 cut(s)	53
MroI	TCCGGA	1 cut(s)	210
MroNI	GCCGGC	2 cut(s)	53, 2557
MroXI	GAANNNNTTC	3 cut(s)	450, 1052, 1087
MseI	TTAA	9 cut(s)	686, 1379, 1400, 1502, 1608, 1947, 2243, 2454, 2532
MslI	CAYNNNNRTG	4 cut(s)	167, 1097, 2024, 2906
MspA1I	CMGCKG	2 cut(s)	1067, 1733
MspI	CCGG	5 cut(s)	54, 114, 211, 2558, 2892
MspR9I	CCNGG	6 cut(s)	115, 468, 1118, 1389, 1413, 2223
MunI	CAATTG	2 cut(s)	315, 2697
Mva1269I	GAATGC	1 cut(s)	562
MvaI	CCWGG	5 cut(s)	468, 1118, 1389, 1413, 2223
MvnI	CGCG	2 cut(s)	245, 261
NaeI	GCCGGC	2 cut(s)	55, 2559
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	1 cut(s)	115
NcoI	CCATGG	2 cut(s)	2808, 2882
NdeI	CATATG	1 cut(s)	2374
NgoMIV	GCCGGC	2 cut(s)	53, 2557
NheI	GCTAGC	2 cut(s)	794, 2069
NlaIV	GGNNCC	9 cut(s)	6, 20, 385, 386, 985, 1090, 1738, 1766, 2172
NmeAIII	GCCGAG	1 cut(s)	33
NmuCI	GTSAC	3 cut(s)	160, 757, 1171
NsiI	ATGCAT	1 cut(s)	2903
NspI	RCATGY	4 cut(s)	423, 640, 1833, 2901
PaeI	GCATGC	2 cut(s)	1833, 2901
PasI	CCCWGGG	1 cut(s)	1117
PciI	ACATGT	1 cut(s)	419
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	1 cut(s)	562
PdiI	GCCGGC	2 cut(s)	55, 2559
PdmI	GAANNNNTTC	3 cut(s)	450, 1052, 1087
PfeI	GAWTC	3 cut(s)	98, 2298, 2628
Pfl23II	CGTACG	1 cut(s)	2578
PleI	GAGTC	3 cut(s)	18, 167, 2630
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	3 cut(s)	18, 167, 2630
Ppu21I	YACGTR	1 cut(s)	1462
PpuMI	RGGWCCY	2 cut(s)	19, 384
PscI	ACATGT	1 cut(s)	419
PshBI	ATTAAT	2 cut(s)	1608, 1947
Psp124BI	GAGCTC	2 cut(s)	1078, 1939
Psp5II	RGGWCCY	2 cut(s)	19, 384
Psp6I	CCWGG	5 cut(s)	466, 1116, 1387, 1411, 2221
PspFI	CCCAGC	1 cut(s)	326
PspGI	CCWGG	5 cut(s)	466, 1116, 1387, 1411, 2221
PspLI	CGTACG	1 cut(s)	2578
PspN4I	GGNNCC	9 cut(s)	6, 20, 385, 386, 985, 1090, 1738, 1766, 2172
PspPI	GGNCC	3 cut(s)	19, 384, 983
PspPPI	RGGWCCY	2 cut(s)	19, 384
PstI	CTGCAG	1 cut(s)	2065
PstNI	CAGNNNCTG	2 cut(s)	1175, 2003
PsuI	RGATCY	2 cut(s)	767, 1580
PvuII	CAGCTG	1 cut(s)	1067
RsaI	GTAC	5 cut(s)	361, 594, 1230, 1417, 2580
RsaNI	GTAC	5 cut(s)	360, 593, 1229, 1416, 2579
RseI	CAYNNNNRTG	4 cut(s)	167, 1097, 2024, 2906
SacI	GAGCTC	2 cut(s)	1078, 1939
SaqAI	TTAA	9 cut(s)	686, 1379, 1400, 1502, 1608, 1947, 2243, 2454, 2532
Sau96I	GGNCC	3 cut(s)	19, 384, 983
SchI	GAGTC	3 cut(s)	19, 168, 2630
ScrFI	CCNGG	6 cut(s)	115, 468, 1118, 1389, 1413, 2223
SduI	GDGCHC	7 cut(s)	231, 1078, 1156, 1454, 1843, 1939, 2175
SfaNI	GCATC	8 cut(s)	313, 559, 643, 844, 1267, 1360, 1912, 2241
SfcI	CTRYAG	5 cut(s)	483, 1311, 1689, 1995, 2061
SfoI	GGCGCC	1 cut(s)	6
SgrAI	CRCCGGYG	1 cut(s)	53
SinI	GGWCC	2 cut(s)	19, 384
SmiMI	CAYNNNNRTG	4 cut(s)	167, 1097, 2024, 2906
SmlI	CTYRAG	2 cut(s)	1453, 2764
SmoI	CTYRAG	2 cut(s)	1453, 2764
SnaBI	TACGTA	1 cut(s)	1462
SphI	GCATGC	2 cut(s)	1833, 2901
SsiI	CCGC	9 cut(s)	51, 234, 259, 867, 1731, 2652, 2840, 2879, 2973
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	5 cut(s)	506, 765, 795, 2070, 2648
SstI	GAGCTC	2 cut(s)	1078, 1939
StyD4I	CCNGG	6 cut(s)	113, 466, 1116, 1387, 1411, 2221
StyI	CCWWGG	4 cut(s)	771, 1984, 2808, 2882
TaaI	ACNGT	6 cut(s)	532, 1233, 1591, 1690, 1761, 1999
TaiI	ACGT	6 cut(s)	176, 270, 764, 1464, 1715, 1956
TaqI	TCGA	8 cut(s)	157, 198, 404, 711, 928, 2196, 2301, 2788
TatI	WGTACW	2 cut(s)	359, 592
TauI	GCSGC	5 cut(s)	869, 1733, 2654, 2843, 2881
TfiI	GAWTC	3 cut(s)	98, 2298, 2628
Tru1I	TTAA	9 cut(s)	686, 1379, 1400, 1502, 1608, 1947, 2243, 2454, 2532
Tru9I	TTAA	9 cut(s)	686, 1379, 1400, 1502, 1608, 1947, 2243, 2454, 2532
TscAI	CASTG	8 cut(s)	169, 913, 1180, 1766, 2080, 2091, 2243, 2868
TseFI	GTSAC	3 cut(s)	160, 757, 1171
TseI	GCWGC	8 cut(s)	34, 179, 376, 425, 1064, 1067, 2429, 2705
Tsp45I	GTSAC	3 cut(s)	160, 757, 1171
TspDTI	ATGAA	6 cut(s)	312, 876, 877, 951, 2148, 2391
TspGWI	ACGGA	3 cut(s)	84, 184, 229
TspRI	CASTG	8 cut(s)	169, 913, 1180, 1766, 2080, 2091, 2243, 2868
VneI	GTGCAC	2 cut(s)	1152, 1839
VpaK11BI	GGWCC	2 cut(s)	19, 384
VspI	ATTAAT	2 cut(s)	1608, 1947
XagI	CCTNNNNNAGG	1 cut(s)	2226
XapI	RAATTY	3 cut(s)	735, 1540, 1652
XbaI	TCTAGA	1 cut(s)	764
XceI	RCATGY	4 cut(s)	423, 640, 1833, 2901
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1816
XmnI	GAANNNNTTC	3 cut(s)	450, 1052, 1087
XspI	CTAG	5 cut(s)	506, 765, 795, 2070, 2648
ZraI	GACGTC	1 cut(s)	268
Zsp2I	ATGCAT	1 cut(s)	2903

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.