Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0005607.1

Orthogroup ID

OG0009249

Physical Location & Seq

Genomic Coordinates Forward (+)

152275 .. 158291

Gene Structure 6017 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0005607.1_g000032.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3009 bp

atggcgccgagtctcagggacctccagctcacgcaggtctccaactccggccgcggcggcggcggcggagacggcggcggtgaagacctcgaggacgtgaggcttttggattcgtatgagaattcggagggagttgaagaagggatgaggacggttcaggttagagttaccgggatgacgtgtgctgcttgctctaattcggttgagggagctcttcagagcgtcaatggcgtgctcagggcctccgtggctttgcttcagaatcgggctgacgtggtttttgatcccagattggtcaaggatgaagatatcaaaaatgcaattgaagatgctgggtttgaggcagaggttatacctgagcccagtgcaaacggagtaaagcagcatggaaacctgtccggtcagttttcgattggaggtatgacatgtgcagcttgcgtgaactctgttgaaggtattctgaaagggcttcctggggtcaaaagagctgtagtagctttagctacttcactaggggaagtggagtatgatcctactgtaattagtaaagatgacatagtcaatgccattgaagatgctggttttgaagcatcttttgtacagagtagtgagcaggataaaattatactgggagttgctggcatattcagtgagatagatgcacaggttttagaagccataatcagcaacttgaaaggggtgagacattttcgtcttgacaggatttcaagagaacttgaaatcctctttgatcctgaagttgtcacttctagatccttggttgatgggattgagggggctagcaatgggaagttcaaacttcaagtagcaaacccttatacaagaatgacttgcaaagatgttgatgaagccgcaaatatgtttcggcttttcatttccagcctggttctcagtgttcctgtctttctcattcgagtagtctgccctcacattccacttctgtattcattattgctctggagatgtgggcctttcgaaatgggtgattggttgaagtgggcattggtgagtgttgttcaatttgtgattggaaagcgcttctacattgcagctgcaagagccctacgaaatggttcaactaacatggatgttctggtcgccttgggaacttcggcctcttacttctactctgtttgtgcacttctatatggtgcaatcactgggttttggtctccgacatactttgaaacaagtgccatgctaataacatttgtactattggggaagtatctggaatgtcttgcaaaaggaaagacatcagatgccatcaaaaagttgatagaactcactccagctacagctttgttgcttgttaaagataaaggtgggagatgtgttggagagagggaaattgatgctttgctcattcagcctggagatacgttaaaagttcttcctggtacaaaagttcctgctgatggtatggttgtgtggggttcaagttatgtaaatgagagtatggtaactggggaagctatacctgtttcaaaagaagtcaacacgttggtgattggaggaacaataaatttacatggtgcccttcacgtacaagttacaaaagttggatctgatacagttttacatcaaataattaatttggtcgagactgctcaaatgtccaaagctcctattcagaaatttgctgattttgttgcaagcatttttgttcctactgtagttgtattgtcattgttgacatttttgggttggtatgttgctggagcctttggagcttacccagaacagtggctccctgaaaatggaaatcactttgtttttgcccttatgttttccatatcagttgtggtcattgcatgcccctgtgcacttggcttggcaacaccaacagctgtcatggtcgcaacaggggttggtgctaataatggtgtgctgataaaaggaggagatgctttggaaagagctcaaaaggttaagtacgtgatatttgataaaacaggcaccctaacccaaggaaaggctacagttactgctgtaaaagttttcacaggaatggatcgcggagattttcttaagttggtggcatctgcagaggctagcagtgaacacccactaggaaaagcaatagttgaatatgcatgccatttccacttctttgatgagccttctgcaaccaatgatgcaacaaaccaaagcaaggagcccctgatctctggatggctttttgatgtctcagatttctctgctttgccaggtagaggcatccagtgctcaataaatggaaaacttattctggttggtaataggaaattgatgactgaaagcggaatcgacattccaacccatgttgaggactttgtagtagagctggaagaaagtgcaaagacaggcatacttgttgcatatgaaggcaatttagttggtgtattgggggttgccgacccactaaaaagagaagctgcaatagtcatagagggacttggtaagatgggtgtcataccagtcatggttacaggagataattggaggacagcacaggctgttgcaaaggaggttggcattaaagatgtgcgagcggaggtaatgccggctggaaaagctgatgttgtacgttcattccaaaaagatggaagcatagttgcaatggttggtgatggaatcaacgactctcctgccctagctgcttctgatgttggtatggcaataggggcagggacagatattgcaattgaagcagctgactatgtattgatgagaaataacttggaagatgtgatcacagccgttgatctctcgagaaagactttcactcgtatccgattgaattatgtgtttgccatggcctacaatgtgattgcaatacctattgccgcaggggttttctttccttcactggggatcatgctgccgccatgggtagccggtgcatgcatggctatgtcatctgttagtgttgtatgctcctctttgctccttaggagatacagaaaaccccgacttactgccatactggaaatagttgtagagtag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0012505 GO:0016020 GO:0023052 GO:0031410 GO:0031982 GO:0031984 GO:0032870 GO:0035556 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071369 GO:0071495 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1002

Amino Acids

106.89

Weight (kDa)

5.12

Isoelectric Point (pI)

29.48

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44790
fragaria_vesca	FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710
malus_domestica	MD05G1247900.v1.1 MD10G1228800.v1.1
prunus_persica	Prupe.4G115900_v2.0.a1 Prupe.4G115900_v2.0.a1
pyrus_communis	pycom05g22420 pycom10g19240
rosa_chinensis	RchiOBHm_Chr5g0020701
rosa_laevigata	RLG00000032554
rosa_multiflora	Rmu_sc0005607.1_g000032
rosa_roxburghii	Rroxscaffold_1G00057600
rosa_rugosa	Rorug05G0062500
rosa_samantha	Rh5AG152200 Rh5BG151900 Rh5CG163300 Rh5DG151400
rosa_wichuraiana	Rw5G013490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	711
AbsI	CCTCGAGG	1 cut(s)	89
Acc36I	ACCTGC	1 cut(s)	25
AccB1I	GGYRCC	3 cut(s)	4, 1556, 1979
AccBSI	CCGCTC	1 cut(s)	2555
AccII	CGCG	2 cut(s)	54, 2040
AclWI	GGATC	7 cut(s)	278, 524, 746, 768, 1594, 2043, 2885
AcoI	YGGCCR	1 cut(s)	49
AcsI	RAATTY	3 cut(s)	121, 1546, 1658
AcuI	CTGAAG	3 cut(s)	200, 242, 777
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	6 cut(s)	600, 1236, 1423, 1569, 1958, 2589
AfeI	AGCGCT	1 cut(s)	1058
AfiI	CCNNNNNNNGG	7 cut(s)	1439, 1781, 1996, 2176, 2237, 2329, 2873
AflII	CTTAAG	1 cut(s)	2051
AflIII	ACRYGT	3 cut(s)	179, 425, 1521
AjiI	CACGTC	3 cut(s)	97, 180, 274
AjnI	CCWGG	5 cut(s)	472, 903, 1393, 1417, 2230
AjuI	GAANNNNNNNTTGG	2 cut(s)	1007, 1039
AleI	CACNNNNGTG	1 cut(s)	1526
AloI	GAACNNNNNNTCC	4 cut(s)	726, 758, 1755, 1787
Alw21I	GWGCWC	6 cut(s)	214, 237, 1162, 1849, 1945, 2252
Alw26I	GTCTC	7 cut(s)	17, 43, 63, 697, 1197, 1619, 2215
Alw44I	GTGCAC	2 cut(s)	1158, 1845
AlwI	GGATC	7 cut(s)	278, 524, 746, 768, 1594, 2043, 2885
AlwNI	CAGNNNCTG	1 cut(s)	2009
Ama87I	CYCGRG	2 cut(s)	89, 2773
Aor51HI	AGCGCT	1 cut(s)	1058
AoxI	GGCC	5 cut(s)	49, 240, 989, 1134, 2820
ApaLI	GTGCAC	2 cut(s)	1158, 1845
ApeKI	GCWGC	9 cut(s)	185, 382, 431, 1070, 1073, 2438, 2660, 2714, 2884
ApoI	RAATTY	3 cut(s)	121, 1546, 1658
ArsI	GACNNNNNNTTYG	2 cut(s)	263, 295
AseI	ATTAAT	1 cut(s)	1614
Asp700I	GAANNNNTTC	2 cut(s)	456, 1093
AspLEI	GCGC	2 cut(s)	7, 1059
AspS9I	GGNCC	3 cut(s)	19, 240, 989
AsuC2I	CCSGG	1 cut(s)	172
AsuHPI	GGTGA	6 cut(s)	92, 712, 1016, 1039, 1540, 2642
AsuII	TTCGAA	1 cut(s)	996
AsuNHI	GCTAGC	2 cut(s)	800, 2075
AvaI	CYCGRG	2 cut(s)	89, 2773
AvaII	GGWCC	1 cut(s)	19
AxyI	CCTNAGG	1 cut(s)	2954
BaeGI	GKGCMC	3 cut(s)	1162, 1561, 1849
BaeI	ACNNNNGTAYC	2 cut(s)	1413, 1446
BanI	GGYRCC	3 cut(s)	4, 1556, 1979
BanII	GRGCYC	5 cut(s)	214, 363, 1084, 1945, 2184
BbsI	GAAGAC	1 cut(s)	90
Bbv12I	GWGCWC	6 cut(s)	214, 237, 1162, 1849, 1945, 2252
BbvI	GCAGC	9 cut(s)	172, 394, 443, 1060, 1082, 2425, 2647, 2726, 2871
BccI	CCATC	7 cut(s)	779, 1295, 1433, 2190, 2461, 2600, 2627
BceAI	ACGGC	2 cut(s)	88, 2747
BcgI	CGANNNNNNTGC	4 cut(s)	924, 958, 2633, 2667
BciT130I	CCWGG	5 cut(s)	474, 905, 1395, 1419, 2232
BciVI	GTATCC	1 cut(s)	2804
BclI	TGATCA	1 cut(s)	2754
BcnI	CCSGG	1 cut(s)	172
BcoDI	GTCTC	7 cut(s)	17, 43, 63, 697, 1197, 1619, 2215
BfaI	CTAG	6 cut(s)	512, 771, 801, 2076, 2093, 2657
BfmI	CTRYAG	5 cut(s)	489, 1317, 1695, 2001, 2067
BfoI	RGCGCY	2 cut(s)	8, 1060
BfrI	CTTAAG	1 cut(s)	2051
BfuAI	ACCTGC	1 cut(s)	25
BfuI	GTATCC	1 cut(s)	2804
BglI	GCCNNNNNGGC	2 cut(s)	57, 248
Bme1390I	CCNGG	6 cut(s)	172, 474, 905, 1395, 1419, 2232
Bme18I	GGWCC	1 cut(s)	19
BmeT110I	CYCGRG	2 cut(s)	89, 2773
BmgBI	CACGTC	3 cut(s)	97, 180, 274
BmgT120I	GGNCC	3 cut(s)	19, 240, 989
BmiI	GGNNCC	7 cut(s)	6, 20, 1558, 1744, 1772, 1981, 2181
BmrFI	CCNGG	6 cut(s)	172, 474, 905, 1395, 1419, 2232
BmrI	ACTGGG	5 cut(s)	357, 638, 1191, 1497, 2882
BmtI	GCTAGC	2 cut(s)	804, 2079
BmuI	ACTGGG	5 cut(s)	357, 638, 1191, 1497, 2882
BpiI	GAAGAC	1 cut(s)	90
BpmI	CTGGAG	5 cut(s)	8, 1000, 1296, 1416, 1761
Bpu10I	CCTNAGC	2 cut(s)	236, 357
Bpu14I	TTCGAA	1 cut(s)	996
BpuMI	CCSGG	1 cut(s)	172
BsaAI	YACGTR	2 cut(s)	1567, 1960
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	2 cut(s)	43, 1197
BsaJI	CCNNGG	8 cut(s)	52, 246, 473, 777, 1122, 1990, 2817, 2891
BsaWI	WCCGGW	1 cut(s)	398
BsaXI	ACNNNNNCTCC	2 cut(s)	1755, 1785
Bsc4I	CCNNNNNNNGG	7 cut(s)	1439, 1781, 1996, 2176, 2237, 2329, 2873
Bse118I	RCCGGY	2 cut(s)	2566, 2900
Bse1I	ACTGG	8 cut(s)	363, 633, 1186, 1492, 2245, 2480, 2877, 2994
Bse21I	CCTNAGG	1 cut(s)	2954
Bse3DI	GCAATG	4 cut(s)	811, 1065, 1830, 2628
BseBI	CCWGG	5 cut(s)	474, 905, 1395, 1419, 2232
BseDI	CCNNGG	8 cut(s)	52, 246, 473, 777, 1122, 1990, 2817, 2891
BseGI	GGATG	6 cut(s)	150, 180, 307, 1114, 2201, 2241
BseLI	CCNNNNNNNGG	7 cut(s)	1439, 1781, 1996, 2176, 2237, 2329, 2873
BseMI	GCAATG	4 cut(s)	811, 1065, 1830, 2628
BseMII	CTCAG	5 cut(s)	28, 250, 348, 925, 2226
BseNI	ACTGG	8 cut(s)	363, 633, 1186, 1492, 2245, 2480, 2877, 2994
BseRI	GAGGAG	2 cut(s)	1938, 2932
BseSI	GKGCMC	3 cut(s)	1162, 1561, 1849
BseX3I	CGGCCG	1 cut(s)	49
BseXI	GCAGC	9 cut(s)	172, 394, 443, 1060, 1082, 2425, 2647, 2726, 2871
BseYI	CCCAGC	1 cut(s)	332
BsgI	GTGCAG	1 cut(s)	450
Bsh1236I	CGCG	2 cut(s)	54, 2040
Bsh1285I	CGRYCG	1 cut(s)	52
BshFI	GGCC	5 cut(s)	51, 242, 991, 1136, 2822
BshNI	GGYRCC	3 cut(s)	4, 1556, 1979
BsiEI	CGRYCG	1 cut(s)	52
BsiHKAI	GWGCWC	6 cut(s)	214, 237, 1162, 1849, 1945, 2252
BsiHKCI	CYCGRG	2 cut(s)	89, 2773
BsiSI	CCGG	5 cut(s)	48, 171, 399, 2567, 2901
BslFI	GGGAC	3 cut(s)	32, 2469, 2707
BslI	CCNNNNNNNGG	7 cut(s)	1439, 1781, 1996, 2176, 2237, 2329, 2873
BsmAI	GTCTC	7 cut(s)	17, 43, 63, 697, 1197, 1619, 2215
BsmBI	CGTCTC	1 cut(s)	63
BsmFI	GGGAC	3 cut(s)	32, 2469, 2707
BsnI	GGCC	5 cut(s)	51, 242, 991, 1136, 2822
Bso31I	GGTCTC	2 cut(s)	43, 1197
BsoBI	CYCGRG	2 cut(s)	89, 2773
Bsp119I	TTCGAA	1 cut(s)	996
Bsp1407I	TGTACA	1 cut(s)	598
Bsp19I	CCATGG	2 cut(s)	2817, 2891
BspANI	GGCC	5 cut(s)	51, 242, 991, 1136, 2822
BspCNI	CTCAG	5 cut(s)	27, 249, 349, 924, 2225
BspFNI	CGCG	2 cut(s)	54, 2040
BspLI	GGNNCC	7 cut(s)	6, 20, 1558, 1744, 1772, 1981, 2181
BspMAI	CTGCAG	1 cut(s)	2071
BspMI	ACCTGC	1 cut(s)	25
BspOI	GCTAGC	2 cut(s)	804, 2079
BspPI	GGATC	7 cut(s)	278, 524, 746, 768, 1594, 2043, 2885
BspQI	GCTCTTC	1 cut(s)	219
BspT104I	TTCGAA	1 cut(s)	996
BspT107I	GGYRCC	3 cut(s)	4, 1556, 1979
BspTI	CTTAAG	1 cut(s)	2051
BspTNI	GGTCTC	2 cut(s)	43, 1197
BsrBI	CCGCTC	1 cut(s)	2555
BsrDI	GCAATG	4 cut(s)	811, 1065, 1830, 2628
BsrFI	RCCGGY	2 cut(s)	2566, 2900
BsrGI	TGTACA	1 cut(s)	598
BsrI	ACTGG	8 cut(s)	363, 633, 1186, 1492, 2245, 2480, 2877, 2994
BssAI	RCCGGY	2 cut(s)	2566, 2900
BssECI	CCNNGG	8 cut(s)	52, 246, 473, 777, 1122, 1990, 2817, 2891
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	5 cut(s)	777, 1122, 1990, 2817, 2891
Bst2UI	CCWGG	5 cut(s)	474, 905, 1395, 1419, 2232
Bst4CI	ACNGT	6 cut(s)	154, 538, 1597, 1696, 1767, 2005
Bst6I	CTCTTC	1 cut(s)	219
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	2051
BstAPI	GCANNNNNTGC	1 cut(s)	2247
BstAUI	TGTACA	1 cut(s)	598
BstBAI	YACGTR	2 cut(s)	1567, 1960
BstBI	TTCGAA	1 cut(s)	996
BstDEI	CTNAG	6 cut(s)	14, 236, 357, 911, 2212, 2954
BstDSI	CCRYGG	4 cut(s)	52, 246, 2817, 2891
BstF5I	GGATG	6 cut(s)	150, 180, 307, 1114, 2201, 2241
BstFNI	CGCG	2 cut(s)	54, 2040
BstH2I	RGCGCY	2 cut(s)	8, 1060
BstHHI	GCGC	2 cut(s)	7, 1059
BstMAI	GTCTC	7 cut(s)	17, 43, 63, 697, 1197, 1619, 2215
BstMCI	CGRYCG	1 cut(s)	52
BstNI	CCWGG	5 cut(s)	474, 905, 1395, 1419, 2232
BstNSI	RCATGY	4 cut(s)	429, 1839, 2121, 2910
BstSCI	CCNGG	6 cut(s)	170, 472, 903, 1393, 1417, 2230
BstSFI	CTRYAG	5 cut(s)	489, 1317, 1695, 2001, 2067
BstSLI	GKGCMC	3 cut(s)	1162, 1561, 1849
BstUI	CGCG	2 cut(s)	54, 2040
BstV1I	GCAGC	9 cut(s)	172, 394, 443, 1060, 1082, 2425, 2647, 2726, 2871
BstV2I	GAAGAC	1 cut(s)	90
BstX2I	RGATCY	2 cut(s)	773, 1586
BstXI	CCANNNNNNTGG	2 cut(s)	1767, 2606
BstYI	RGATCY	2 cut(s)	773, 1586
BstZI	CGGCCG	1 cut(s)	49
Bsu36I	CCTNAGG	1 cut(s)	2954
BsuI	GTATCC	1 cut(s)	2804
BsuRI	GGCC	5 cut(s)	51, 242, 991, 1136, 2822
BtgI	CCRYGG	4 cut(s)	52, 246, 2817, 2891
BtrI	CACGTC	3 cut(s)	97, 180, 274
BtsCI	GGATG	6 cut(s)	150, 180, 307, 1114, 2201, 2241
BtsI	GCAGTG	1 cut(s)	2086
BtsIMutI	CAGTG	8 cut(s)	370, 655, 919, 1179, 1772, 2086, 2252, 2870
BveI	ACCTGC	1 cut(s)	25
CaiI	CAGNNNCTG	1 cut(s)	2009
CfoI	GCGC	2 cut(s)	7, 1059
Cfr10I	RCCGGY	2 cut(s)	2566, 2900
Cfr13I	GGNCC	3 cut(s)	19, 240, 989
Cfr42I	CCGCGG	1 cut(s)	55
CseI	GACGC	1 cut(s)	211
Csp6I	GTAC	6 cut(s)	599, 1235, 1422, 1568, 1957, 2588
CviQI	GTAC	6 cut(s)	599, 1235, 1422, 1568, 1957, 2588
DdeI	CTNAG	6 cut(s)	14, 236, 357, 911, 2212, 2954
DinI	GGCGCC	1 cut(s)	6
DrdI	GACNNNNNNGTC	1 cut(s)	711
DseDI	GACNNNNNNGTC	1 cut(s)	711
EaeI	YGGCCR	1 cut(s)	49
EagI	CGGCCG	1 cut(s)	49
Eam1104I	CTCTTC	1 cut(s)	219
EarI	CTCTTC	1 cut(s)	219
EciI	GGCGGA	1 cut(s)	81
Ecl136II	GAGCTC	2 cut(s)	212, 1943
EclXI	CGGCCG	1 cut(s)	49
Eco130I	CCWWGG	5 cut(s)	777, 1122, 1990, 2817, 2891
Eco24I	GRGCYC	5 cut(s)	214, 363, 1084, 1945, 2184
Eco31I	GGTCTC	2 cut(s)	43, 1197
Eco32I	GATATC	1 cut(s)	310
Eco47I	GGWCC	1 cut(s)	19
Eco47III	AGCGCT	1 cut(s)	1058
Eco52I	CGGCCG	1 cut(s)	49
Eco53kI	GAGCTC	2 cut(s)	212, 1943
Eco57I	CTGAAG	3 cut(s)	200, 242, 777
Eco81I	CCTNAGG	1 cut(s)	2954
Eco88I	CYCGRG	2 cut(s)	89, 2773
EcoICRI	GAGCTC	2 cut(s)	212, 1943
EcoO109I	RGGNCCY	2 cut(s)	19, 240
EcoRI	GAATTC	1 cut(s)	121
EcoRII	CCWGG	5 cut(s)	472, 903, 1393, 1417, 2230
EcoRV	GATATC	1 cut(s)	310
EcoT14I	CCWWGG	5 cut(s)	777, 1122, 1990, 2817, 2891
EcoT22I	ATGCAT	2 cut(s)	2119, 2912
EcoT38I	GRGCYC	5 cut(s)	214, 363, 1084, 1945, 2184
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	5 cut(s)	777, 1122, 1990, 2817, 2891
Esp3I	CGTCTC	1 cut(s)	63
FalI	AAGNNNNNCTT	2 cut(s)	835, 867
FaqI	GGGAC	3 cut(s)	32, 2469, 2707
FauNDI	CATATG	1 cut(s)	2383
FbaI	TGATCA	1 cut(s)	2754
FokI	GGATG	6 cut(s)	157, 187, 314, 1121, 2208, 2228
FriOI	GRGCYC	5 cut(s)	214, 363, 1084, 1945, 2184
FspBI	CTAG	6 cut(s)	512, 771, 801, 2076, 2093, 2657
GlaI	GCGC	2 cut(s)	6, 1058
GsaI	CCCAGC	1 cut(s)	336
GsuI	CTGGAG	5 cut(s)	8, 1000, 1296, 1416, 1761
HaeII	RGCGCY	2 cut(s)	8, 1060
HaeIII	GGCC	5 cut(s)	51, 242, 991, 1136, 2822
HapII	CCGG	5 cut(s)	48, 171, 399, 2567, 2901
HgaI	GACGC	1 cut(s)	211
HhaI	GCGC	2 cut(s)	7, 1059
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	2 cut(s)	5, 1057
HinP1I	GCGC	2 cut(s)	5, 1057
HincII	GTYRAC	2 cut(s)	1519, 1716
HindII	GTYRAC	2 cut(s)	1519, 1716
HinfI	GANTC	6 cut(s)	10, 110, 262, 2307, 2637, 2645
HpaII	CCGG	5 cut(s)	48, 171, 399, 2567, 2901
HphI	GGTGA	6 cut(s)	92, 712, 1016, 1039, 1540, 2642
Hpy166II	GTNNAC	6 cut(s)	442, 1160, 1519, 1716, 1847, 2084
Hpy8I	GTNNAC	6 cut(s)	442, 1160, 1519, 1716, 1847, 2084
HpyAV	CCTTC	6 cut(s)	134, 446, 1571, 2154, 2381, 2877
HpyCH4III	ACNGT	6 cut(s)	154, 538, 1597, 1696, 1767, 2005
HpyCH4IV	ACGT	8 cut(s)	96, 179, 273, 1403, 1523, 1566, 1959, 2590
HpyF3I	CTNAG	6 cut(s)	14, 236, 357, 911, 2212, 2954
HpySE526I	ACGT	8 cut(s)	96, 179, 273, 1403, 1523, 1566, 1959, 2590
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	2 cut(s)	5, 1057
KasI	GGCGCC	1 cut(s)	4
KroI	GCCGGC	1 cut(s)	2566
KroNI	GCCGGC	1 cut(s)	2568
Ksp22I	TGATCA	1 cut(s)	2754
KspI	CCGCGG	1 cut(s)	55
LguI	GCTCTTC	1 cut(s)	219
LmnI	GCTCC	8 cut(s)	209, 1651, 1742, 1751, 1776, 2179, 2945, 2955
Lsp1109I	GCAGC	9 cut(s)	172, 394, 443, 1060, 1082, 2425, 2647, 2726, 2871
MaeI	CTAG	6 cut(s)	512, 771, 801, 2076, 2093, 2657
MaeII	ACGT	8 cut(s)	96, 179, 273, 1403, 1523, 1566, 1959, 2590
MaeIII	GTNAC	6 cut(s)	166, 763, 1483, 1573, 2005, 2488
MbiI	CCGCTC	1 cut(s)	2555
MboII	GAAGA	9 cut(s)	95, 149, 206, 317, 338, 584, 1406, 2363, 2759
MfeI	CAATTG	2 cut(s)	321, 2706
MflI	RGATCY	2 cut(s)	773, 1586
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	19, 2639
MmeI	TCCRAC	5 cut(s)	66, 1220, 1339, 1564, 2342
Mph1103I	ATGCAT	2 cut(s)	2119, 2912
MroNI	GCCGGC	1 cut(s)	2566
MroXI	GAANNNNTTC	2 cut(s)	456, 1093
MseI	TTAA	6 cut(s)	1335, 1406, 1614, 1953, 2052, 2541
MslI	CAYNNNNRTG	3 cut(s)	1526, 2030, 2915
MspA1I	CMGCKG	4 cut(s)	54, 1073, 1871, 2717
MspCI	CTTAAG	1 cut(s)	2051
MspI	CCGG	5 cut(s)	48, 171, 399, 2567, 2901
MspR9I	CCNGG	6 cut(s)	172, 474, 905, 1395, 1419, 2232
MunI	CAATTG	2 cut(s)	321, 2706
MvaI	CCWGG	5 cut(s)	474, 905, 1395, 1419, 2232
MvnI	CGCG	2 cut(s)	54, 2040
NaeI	GCCGGC	1 cut(s)	2568
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	1 cut(s)	172
NcoI	CCATGG	2 cut(s)	2817, 2891
NdeI	CATATG	1 cut(s)	2383
NgoMIV	GCCGGC	1 cut(s)	2566
NheI	GCTAGC	2 cut(s)	800, 2075
NlaIV	GGNNCC	7 cut(s)	6, 20, 1558, 1744, 1772, 1981, 2181
NmeAIII	GCCGAG	1 cut(s)	33
NmuCI	GTSAC	1 cut(s)	763
NsiI	ATGCAT	2 cut(s)	2119, 2912
NspI	RCATGY	4 cut(s)	429, 1839, 2121, 2910
NspV	TTCGAA	1 cut(s)	996
OliI	CACNNNNGTG	1 cut(s)	1526
PaeI	GCATGC	3 cut(s)	1839, 2121, 2910
PaeR7I	CTCGAG	2 cut(s)	89, 2773
PciI	ACATGT	1 cut(s)	425
PciSI	GCTCTTC	1 cut(s)	219
PdiI	GCCGGC	1 cut(s)	2568
PdmI	GAANNNNTTC	2 cut(s)	456, 1093
PfeI	GAWTC	4 cut(s)	110, 262, 2307, 2637
PflFI	GACNNNGTC	1 cut(s)	557
PleI	GAGTC	2 cut(s)	18, 2639
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	2 cut(s)	18, 2639
Ppu21I	YACGTR	2 cut(s)	1567, 1960
PpuMI	RGGWCCY	1 cut(s)	19
PscI	ACATGT	1 cut(s)	425
PshBI	ATTAAT	1 cut(s)	1614
Psp124BI	GAGCTC	2 cut(s)	214, 1945
Psp5II	RGGWCCY	1 cut(s)	19
Psp6I	CCWGG	5 cut(s)	472, 903, 1393, 1417, 2230
PspFI	CCCAGC	1 cut(s)	332
PspGI	CCWGG	5 cut(s)	472, 903, 1393, 1417, 2230
PspN4I	GGNNCC	7 cut(s)	6, 20, 1558, 1744, 1772, 1981, 2181
PspPI	GGNCC	3 cut(s)	19, 240, 989
PspPPI	RGGWCCY	1 cut(s)	19
PspXI	VCTCGAGB	1 cut(s)	89
PstI	CTGCAG	1 cut(s)	2071
PstNI	CAGNNNCTG	1 cut(s)	2009
PsuI	RGATCY	2 cut(s)	773, 1586
PsyI	GACNNNGTC	1 cut(s)	557
PvuII	CAGCTG	3 cut(s)	1073, 1871, 2717
RsaI	GTAC	6 cut(s)	600, 1236, 1423, 1569, 1958, 2589
RsaNI	GTAC	6 cut(s)	599, 1235, 1422, 1568, 1957, 2588
RseI	CAYNNNNRTG	3 cut(s)	1526, 2030, 2915
SacI	GAGCTC	2 cut(s)	214, 1945
SacII	CCGCGG	1 cut(s)	55
SapI	GCTCTTC	1 cut(s)	219
SaqAI	TTAA	6 cut(s)	1335, 1406, 1614, 1953, 2052, 2541
Sau96I	GGNCC	3 cut(s)	19, 240, 989
SchI	GAGTC	2 cut(s)	19, 2639
ScrFI	CCNGG	6 cut(s)	172, 474, 905, 1395, 1419, 2232
SfcI	CTRYAG	5 cut(s)	489, 1317, 1695, 2001, 2067
SfoI	GGCGCC	1 cut(s)	6
Sfr274I	CTCGAG	2 cut(s)	89, 2773
Sfr303I	CCGCGG	1 cut(s)	55
SfuI	TTCGAA	1 cut(s)	996
SgrBI	CCGCGG	1 cut(s)	55
SinI	GGWCC	1 cut(s)	19
SlaI	CTCGAG	2 cut(s)	89, 2773
SmiMI	CAYNNNNRTG	3 cut(s)	1526, 2030, 2915
SmlI	CTYRAG	3 cut(s)	89, 2051, 2773
SmoI	CTYRAG	3 cut(s)	89, 2051, 2773
SphI	GCATGC	3 cut(s)	1839, 2121, 2910
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	6 cut(s)	512, 771, 801, 2076, 2093, 2657
SstI	GAGCTC	2 cut(s)	214, 1945
StyD4I	CCNGG	6 cut(s)	170, 472, 903, 1393, 1417, 2230
StyI	CCWWGG	5 cut(s)	777, 1122, 1990, 2817, 2891
TaaI	ACNGT	6 cut(s)	154, 538, 1597, 1696, 1767, 2005
TaiI	ACGT	8 cut(s)	99, 182, 276, 1406, 1526, 1569, 1962, 2593
TaqI	TCGA	7 cut(s)	90, 410, 934, 996, 1623, 2310, 2774
TatI	WGTACW	2 cut(s)	598, 1234
TauI	GCSGC	9 cut(s)	54, 57, 60, 63, 66, 78, 875, 2852, 2890
TfiI	GAWTC	4 cut(s)	110, 262, 2307, 2637
Tru1I	TTAA	6 cut(s)	1335, 1406, 1614, 1953, 2052, 2541
Tru9I	TTAA	6 cut(s)	1335, 1406, 1614, 1953, 2052, 2541
TscAI	CASTG	8 cut(s)	370, 655, 919, 1186, 1772, 2086, 2252, 2877
TseFI	GTSAC	1 cut(s)	763
TseI	GCWGC	9 cut(s)	185, 382, 431, 1070, 1073, 2438, 2660, 2714, 2884
Tsp45I	GTSAC	1 cut(s)	763
TspDTI	ATGAA	6 cut(s)	318, 882, 883, 957, 2400, 2583
TspGWI	ACGGA	2 cut(s)	235, 387
TspRI	CASTG	8 cut(s)	370, 655, 919, 1186, 1772, 2086, 2252, 2877
Tth111I	GACNNNGTC	1 cut(s)	557
Vha464I	CTTAAG	1 cut(s)	2051
VneI	GTGCAC	2 cut(s)	1158, 1845
VpaK11BI	GGWCC	1 cut(s)	19
VspI	ATTAAT	1 cut(s)	1614
XapI	RAATTY	3 cut(s)	121, 1546, 1658
XbaI	TCTAGA	1 cut(s)	770
XceI	RCATGY	4 cut(s)	429, 1839, 2121, 2910
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1822
XhoI	CTCGAG	2 cut(s)	89, 2773
XmnI	GAANNNNTTC	2 cut(s)	456, 1093
XspI	CTAG	6 cut(s)	512, 771, 801, 2076, 2093, 2657
Zsp2I	ATGCAT	2 cut(s)	2119, 2912

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.