Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0009249

Physical Location & Seq

Genomic Coordinates Forward (+)

15866647 .. 15873071

Gene Structure 6425 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G013490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3012 bp

ATGGCGCCGAGTCTCAGGGACCTCCAGCTCACGCAGGTCTGGAACTCCGGCCGCGGCGGCGGCGGCGACGACGGCGGCGGCGGTGAAGGCCTCGAGGACGTGAGGCTTTTGGATTCGTATGAGAATTCGGAGGGAGTTGAAGAAGGGATGAGGACGGTTCAGGTTAGAGTTACCGGGATGACGTGTGCTGCTTGCTCTAATTCGGTTGAGGGAGCTCTCCAGAGCGTCAATGGCGTGCTCAGGGCCTCCGTGGCTTTGCTTCAGAATCGGGCTGACGTGGTTTTTGATCCCAGATTGGTCAAGGATGAAGATATCAAAAATGCAATTGAAGATGCTGGGTTTGAGGCAGAGGTTATACCTGAGCCCAGTGCAAACGGAGTAAAGCAGCATGGAAACCTGTCTGGTCAGTTTTCGATTGGAGGTATGACATGTGCAGCTTGCGTGAACTCTGTTGAAGGTATTCTGAAAGGGCTTCCTGGGGTCAAAAGAGCTGTAGTAGCTTTAGCTACTTCACTAGGGGAAGTGGAGTATGATCCTACTGTAATTAGTAAAGATGACATAGTCAATGCCATTGAAGATGCTGGTTTTGAAGCATCTTTTGTACAGAGTAGTGAGCAGGATAAAATTATACTGGGAGTTACTGGCATATTCAGTGAGATAGATGCACAGGTTTTAGAAGCCATAATCAGCAACTTGAAAGGGGTGAGACATTTTCGTCTTGACAGGATTTCAAGAGAACTTGAAATCCTCTTTGATCCTGAAGTTGTCACTTCTAGATCCTTGGTTGATGGGATTGAGGGGGCTAGCAATGGGAAGTTCAAACTTCAAGTAGCAAACCCTTATACAAGAATGACTTGCAAAGATGTTGATGAAGCCGCAAATATGTTTCGGCTTTTCATTTCCAGCCTGGTTCTCAGTGTTCCTGTCTTTCTCATTCGAGTAGTCTGCCCTCACATTCCACTTCTGTATTCATTATTGCTCTGGAGATGTGGGCCTTTCGAAATGGGTGATTGGTTGAAGTGGGCATTGGTGAGTGTTGTTCAATTTGTGATTGGAAAGCGCTTCTACATTGCAGCTGCAAGAGCCCTACGAAATGGTTCAACTAACATGGATGTTCTGGTCGCCTTGGGAACTTCGGCCTCTTACTTCTACTCTGTTTGTGCACTTCTATATGGTGCAATCACTGGGTTTTGGTCTCCGACATACTTTGAAACAAGTGCCATGCTAATAACATTTGTACTATTGGGGAAGTATCTGGAATGTCTTGCAAAAGGAAAGACATCAGATGCCATCAAAAAGTTGATAGAACTCACTCCAGCTACAGCTTTGTTGCTTGTTAAAGATAAAGGTGGGAGATGTGTTGGAGAGAGGGAAATTGATGCTTTGCTCATTCAGCCTGGAGATACGTTAAAAGTTCTTCCTGGTACAAAAGTTCCTGCTGATGGTATGGTTGTGTGGGGTTCAAGTTATGTAAATGAGAGTATGGTAACTGGGGAAGCTATACCTGTTTCAAAAGAAGTCGACACGTTGGTGATTGGAGGAACAATAAATTTACATGGTGCCCTTCACGTACAAGTTACAAAAGTTGGATCTGATACAGTTTTACATCAAATAATTAATTTGGTCGAGACTGCTCAAATGTCCAAAGCTCCTATTCAGAAATTTGCTGATTTTGTTGCAAGCATTTTTGTTCCTACTGTAGTTGTATTGTCATTGTTGACATTTTTGGGTTGGTATGTTGCTGGAGCCTTTGGAGCTTACCCAGAACAGTGGCTCCCTGAAAATGGAAATCACTTTGTTTTTGCCCTTATGTTTTCCATATCAGTTGTGGTCATTGCATGCCCCTGTGCACTTGGCTTGGCAACACCAACAGCTGTCATGGTCGCAACAGGGGTTGGTGCTAATAATGGTGTGCTGATAAAAGGAGGAGATGCTTTGGAAAGAGCTCAAAAGGTTAAGTACGTGATATTTGATAAAACAGGCACCCTAACCCAAGGAAAGGCTACAGTTACTGCTGTAAAAGTTTTCACAGGAATGGATCGCGGAGATTTTCTTAAGTTGGTGGCATCTGCAGAGGCTAGCAGTGAACACCCACTAGGAAAAGCAATAGTTGAATATGCACGCCATTTCCACTTCTTTGATGAGCCTTCTGCAACCAATGATGCAACAAACCAAAGCAAGGAGCCCCTGATCTCTGGATGGCTTTTTGATGTCTCAGATTTCTCTGCTTTGCCAGGTAGAGGCATCCAGTGCTCAATAAATGGAAAACTTATTCTGGTTGGTAATAGGAAATTGATGACTGAAAGCGGAATCGACATTCCAACCCATGTTGAGGACTTTGTAGTAGAGCTGGAAGAAAGTGCAAAGACAGGCATACTTGTTGCATATGAAGGCAATTTAGTTGGTGTATTGGGGGTTGCCGACCCACTAAAAAGAGAAGCTGCAATAGTCATAGAGGGACTTGGTAAGATGGGTGTCATACCAGTCATGGTTACAGGAGATAATTGGAGGACAGCACAGGCTGTTGCAAAGGAGGTTGGCATTAAAGATGTGCGAGCGGAGGTAATGCCGGCTGGAAAAGCTGATGTTGTACGTTCATTCCAAAAAGATGGAAGCATAGTTGCAATGGTTGGTGATGGAATCAACGACTCTCCTGCCCTAGCTGCTTCTGATGTTGGTATGGCAATAGGGGCAGGGACAGATATTGCAATTGAAGCAGCTGACTATGTATTGATGAGAAATAACTTGGAAGATGTGATCACAGCCGTTGATCTCTCGAGAAAGACTTTCACTCGTATCCGATTGAATTATGTGTTTGCCATGGCCTACAATGTGATTGCAATACCTATTGCCGCAGGGGTTTTCTTTCCTTCACTGGGGATCATGCTGCCGCCATGGGTAGCCGGTGCATGCATGGCTATGTCATCTGTTAGTGTTGTATGCTCCTCTTTGCTCCTTAGGAGATACAGAAAACCCCGACTTACTGCCATACTGGAAATAGTTGTAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0012505 GO:0016020 GO:0023052 GO:0031410 GO:0031982 GO:0031984 GO:0032870 GO:0035556 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071369 GO:0071495 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1003

Amino Acids

107.13

Weight (kDa)

5.12

Isoelectric Point (pI)

28.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	55 - 115	6e-13	Heavy-metal-associated domain
HMA	PF00403	137 - 197	2.2e-15	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	438 - 543	2.2e-24	P-type ATPase actuator domain
Hydrolase	PF00702	652 - 890	1.6e-29	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44790
fragaria_vesca	FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710
malus_domestica	MD05G1247900.v1.1 MD10G1228800.v1.1
prunus_persica	Prupe.4G115900_v2.0.a1 Prupe.4G115900_v2.0.a1
pyrus_communis	pycom05g22420 pycom10g19240
rosa_chinensis	RchiOBHm_Chr5g0020701
rosa_laevigata	RLG00000032554
rosa_multiflora	Rmu_sc0005607.1_g000032
rosa_roxburghii	Rroxscaffold_1G00057600
rosa_rugosa	Rorug05G0062500
rosa_samantha	Rh5AG152200 Rh5BG151900 Rh5CG163300 Rh5DG151400
rosa_wichuraiana	Rw5G013490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	714
AbsI	CCTCGAGG	1 cut(s)	92
Acc36I	ACCTGC	1 cut(s)	25
AccB1I	GGYRCC	3 cut(s)	4, 1559, 1982
AccBSI	CCGCTC	1 cut(s)	2558
AccI	GTMKAC	1 cut(s)	1521
AccII	CGCG	2 cut(s)	54, 2043
AclWI	GGATC	7 cut(s)	281, 527, 749, 771, 1597, 2046, 2888
AcoI	YGGCCR	1 cut(s)	49
AcsI	RAATTY	3 cut(s)	124, 1549, 1661
AcuI	CTGAAG	2 cut(s)	245, 780
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	6 cut(s)	603, 1239, 1426, 1572, 1961, 2592
AfeI	AGCGCT	1 cut(s)	1061
AfiI	CCNNNNNNNGG	7 cut(s)	1442, 1784, 1999, 2179, 2240, 2332, 2876
AflII	CTTAAG	1 cut(s)	2054
AflIII	ACRYGT	3 cut(s)	182, 428, 1524
AjiI	CACGTC	3 cut(s)	100, 183, 277
AjnI	CCWGG	5 cut(s)	475, 906, 1396, 1420, 2233
AjuI	GAANNNNNNNTTGG	2 cut(s)	1010, 1042
AleI	CACNNNNGTG	1 cut(s)	1529
AloI	GAACNNNNNNTCC	4 cut(s)	729, 761, 1758, 1790
Alw21I	GWGCWC	6 cut(s)	217, 240, 1165, 1852, 1948, 2255
Alw26I	GTCTC	5 cut(s)	17, 700, 1200, 1622, 2218
Alw44I	GTGCAC	2 cut(s)	1161, 1848
AlwI	GGATC	7 cut(s)	281, 527, 749, 771, 1597, 2046, 2888
AlwNI	CAGNNNCTG	1 cut(s)	2012
Ama87I	CYCGRG	2 cut(s)	92, 2776
Aor51HI	AGCGCT	1 cut(s)	1061
AoxI	GGCC	6 cut(s)	49, 88, 243, 992, 1137, 2823
ApaLI	GTGCAC	2 cut(s)	1161, 1848
ApeKI	GCWGC	9 cut(s)	188, 385, 434, 1073, 1076, 2441, 2663, 2717, 2887
ApoI	RAATTY	3 cut(s)	124, 1549, 1661
ArsI	GACNNNNNNTTYG	2 cut(s)	266, 298
AseI	ATTAAT	1 cut(s)	1617
Asp700I	GAANNNNTTC	2 cut(s)	459, 1096
AspLEI	GCGC	2 cut(s)	7, 1062
AspS9I	GGNCC	3 cut(s)	19, 243, 992
AsuC2I	CCSGG	1 cut(s)	175
AsuHPI	GGTGA	6 cut(s)	95, 715, 1019, 1042, 1543, 2645
AsuII	TTCGAA	1 cut(s)	999
AsuNHI	GCTAGC	2 cut(s)	803, 2078
AvaI	CYCGRG	2 cut(s)	92, 2776
AvaII	GGWCC	1 cut(s)	19
AxyI	CCTNAGG	1 cut(s)	2957
BaeGI	GKGCMC	3 cut(s)	1165, 1564, 1852
BaeI	ACNNNNGTAYC	2 cut(s)	1416, 1449
BanI	GGYRCC	3 cut(s)	4, 1559, 1982
BanII	GRGCYC	5 cut(s)	217, 366, 1087, 1948, 2187
Bbv12I	GWGCWC	6 cut(s)	217, 240, 1165, 1852, 1948, 2255
BbvI	GCAGC	9 cut(s)	175, 397, 446, 1063, 1085, 2428, 2650, 2729, 2874
BccI	CCATC	7 cut(s)	782, 1298, 1436, 2193, 2464, 2603, 2630
BceAI	ACGGC	2 cut(s)	88, 2750
BcgI	CGANNNNNNTGC	4 cut(s)	927, 961, 2636, 2670
BciT130I	CCWGG	5 cut(s)	477, 908, 1398, 1422, 2235
BciVI	GTATCC	1 cut(s)	2807
BclI	TGATCA	1 cut(s)	2757
BcnI	CCSGG	1 cut(s)	175
BcoDI	GTCTC	5 cut(s)	17, 700, 1200, 1622, 2218
BfaI	CTAG	6 cut(s)	515, 774, 804, 2079, 2096, 2660
BfmI	CTRYAG	5 cut(s)	492, 1320, 1698, 2004, 2070
BfoI	RGCGCY	2 cut(s)	8, 1063
BfrI	CTTAAG	1 cut(s)	2054
BfuAI	ACCTGC	1 cut(s)	25
BfuI	GTATCC	1 cut(s)	2807
BglI	GCCNNNNNGGC	2 cut(s)	57, 251
Bme1390I	CCNGG	6 cut(s)	175, 477, 908, 1398, 1422, 2235
Bme18I	GGWCC	1 cut(s)	19
BmeT110I	CYCGRG	2 cut(s)	92, 2776
BmgBI	CACGTC	3 cut(s)	100, 183, 277
BmgT120I	GGNCC	3 cut(s)	19, 243, 992
BmiI	GGNNCC	7 cut(s)	6, 20, 1561, 1747, 1775, 1984, 2184
BmrFI	CCNGG	6 cut(s)	175, 477, 908, 1398, 1422, 2235
BmrI	ACTGGG	5 cut(s)	360, 641, 1194, 1500, 2885
BmtI	GCTAGC	2 cut(s)	807, 2082
BmuI	ACTGGG	5 cut(s)	360, 641, 1194, 1500, 2885
BpmI	CTGGAG	6 cut(s)	8, 203, 1003, 1299, 1419, 1764
Bpu10I	CCTNAGC	2 cut(s)	239, 360
Bpu14I	TTCGAA	1 cut(s)	999
BpuMI	CCSGG	1 cut(s)	175
BsaAI	YACGTR	2 cut(s)	1570, 1963
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	1200
BsaJI	CCNNGG	8 cut(s)	52, 249, 476, 780, 1125, 1993, 2820, 2894
BsaXI	ACNNNNNCTCC	2 cut(s)	1758, 1788
Bsc4I	CCNNNNNNNGG	7 cut(s)	1442, 1784, 1999, 2179, 2240, 2332, 2876
Bse118I	RCCGGY	2 cut(s)	2569, 2903
Bse1I	ACTGG	9 cut(s)	366, 636, 646, 1189, 1495, 2248, 2483, 2880, 2997
Bse21I	CCTNAGG	1 cut(s)	2957
Bse3DI	GCAATG	4 cut(s)	814, 1068, 1833, 2631
BseBI	CCWGG	5 cut(s)	477, 908, 1398, 1422, 2235
BseDI	CCNNGG	8 cut(s)	52, 249, 476, 780, 1125, 1993, 2820, 2894
BseGI	GGATG	6 cut(s)	153, 183, 310, 1117, 2204, 2244
BseLI	CCNNNNNNNGG	7 cut(s)	1442, 1784, 1999, 2179, 2240, 2332, 2876
BseMI	GCAATG	4 cut(s)	814, 1068, 1833, 2631
BseMII	CTCAG	5 cut(s)	28, 253, 351, 928, 2229
BseNI	ACTGG	9 cut(s)	366, 636, 646, 1189, 1495, 2248, 2483, 2880, 2997
BseRI	GAGGAG	2 cut(s)	1941, 2935
BseSI	GKGCMC	3 cut(s)	1165, 1564, 1852
BseX3I	CGGCCG	1 cut(s)	49
BseXI	GCAGC	9 cut(s)	175, 397, 446, 1063, 1085, 2428, 2650, 2729, 2874
BseYI	CCCAGC	1 cut(s)	335
BsgI	GTGCAG	1 cut(s)	453
Bsh1236I	CGCG	2 cut(s)	54, 2043
Bsh1285I	CGRYCG	1 cut(s)	52
BshFI	GGCC	6 cut(s)	51, 90, 245, 994, 1139, 2825
BshNI	GGYRCC	3 cut(s)	4, 1559, 1982
BsiEI	CGRYCG	1 cut(s)	52
BsiHKAI	GWGCWC	6 cut(s)	217, 240, 1165, 1852, 1948, 2255
BsiHKCI	CYCGRG	2 cut(s)	92, 2776
BsiSI	CCGG	4 cut(s)	48, 174, 2570, 2904
BslFI	GGGAC	3 cut(s)	32, 2472, 2710
BslI	CCNNNNNNNGG	7 cut(s)	1442, 1784, 1999, 2179, 2240, 2332, 2876
BsmAI	GTCTC	5 cut(s)	17, 700, 1200, 1622, 2218
BsmFI	GGGAC	3 cut(s)	32, 2472, 2710
BsnI	GGCC	6 cut(s)	51, 90, 245, 994, 1139, 2825
Bso31I	GGTCTC	1 cut(s)	1200
BsoBI	CYCGRG	2 cut(s)	92, 2776
Bsp119I	TTCGAA	1 cut(s)	999
Bsp1407I	TGTACA	1 cut(s)	601
Bsp19I	CCATGG	2 cut(s)	2820, 2894
BspANI	GGCC	6 cut(s)	51, 90, 245, 994, 1139, 2825
BspCNI	CTCAG	5 cut(s)	27, 252, 352, 927, 2228
BspFNI	CGCG	2 cut(s)	54, 2043
BspLI	GGNNCC	7 cut(s)	6, 20, 1561, 1747, 1775, 1984, 2184
BspMAI	CTGCAG	1 cut(s)	2074
BspMI	ACCTGC	1 cut(s)	25
BspOI	GCTAGC	2 cut(s)	807, 2082
BspPI	GGATC	7 cut(s)	281, 527, 749, 771, 1597, 2046, 2888
BspT104I	TTCGAA	1 cut(s)	999
BspT107I	GGYRCC	3 cut(s)	4, 1559, 1982
BspTI	CTTAAG	1 cut(s)	2054
BspTNI	GGTCTC	1 cut(s)	1200
BsrBI	CCGCTC	1 cut(s)	2558
BsrDI	GCAATG	4 cut(s)	814, 1068, 1833, 2631
BsrFI	RCCGGY	2 cut(s)	2569, 2903
BsrGI	TGTACA	1 cut(s)	601
BsrI	ACTGG	9 cut(s)	366, 636, 646, 1189, 1495, 2248, 2483, 2880, 2997
BssAI	RCCGGY	2 cut(s)	2569, 2903
BssECI	CCNNGG	8 cut(s)	52, 249, 476, 780, 1125, 1993, 2820, 2894
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	5 cut(s)	780, 1125, 1993, 2820, 2894
Bst2UI	CCWGG	5 cut(s)	477, 908, 1398, 1422, 2235
Bst4CI	ACNGT	6 cut(s)	157, 541, 1600, 1699, 1770, 2008
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	2054
BstAPI	GCANNNNNTGC	1 cut(s)	2250
BstAUI	TGTACA	1 cut(s)	601
BstBAI	YACGTR	2 cut(s)	1570, 1963
BstBI	TTCGAA	1 cut(s)	999
BstDEI	CTNAG	6 cut(s)	14, 239, 360, 914, 2215, 2957
BstDSI	CCRYGG	4 cut(s)	52, 249, 2820, 2894
BstF5I	GGATG	6 cut(s)	153, 183, 310, 1117, 2204, 2244
BstFNI	CGCG	2 cut(s)	54, 2043
BstH2I	RGCGCY	2 cut(s)	8, 1063
BstHHI	GCGC	2 cut(s)	7, 1062
BstMAI	GTCTC	5 cut(s)	17, 700, 1200, 1622, 2218
BstMCI	CGRYCG	1 cut(s)	52
BstNI	CCWGG	5 cut(s)	477, 908, 1398, 1422, 2235
BstNSI	RCATGY	3 cut(s)	432, 1842, 2913
BstSCI	CCNGG	6 cut(s)	173, 475, 906, 1396, 1420, 2233
BstSFI	CTRYAG	5 cut(s)	492, 1320, 1698, 2004, 2070
BstSLI	GKGCMC	3 cut(s)	1165, 1564, 1852
BstUI	CGCG	2 cut(s)	54, 2043
BstV1I	GCAGC	9 cut(s)	175, 397, 446, 1063, 1085, 2428, 2650, 2729, 2874
BstX2I	RGATCY	2 cut(s)	776, 1589
BstXI	CCANNNNNNTGG	2 cut(s)	1770, 2609
BstYI	RGATCY	2 cut(s)	776, 1589
BstZI	CGGCCG	1 cut(s)	49
Bsu36I	CCTNAGG	1 cut(s)	2957
BsuI	GTATCC	1 cut(s)	2807
BsuRI	GGCC	6 cut(s)	51, 90, 245, 994, 1139, 2825
BtgI	CCRYGG	4 cut(s)	52, 249, 2820, 2894
BtrI	CACGTC	3 cut(s)	100, 183, 277
BtsCI	GGATG	6 cut(s)	153, 183, 310, 1117, 2204, 2244
BtsI	GCAGTG	1 cut(s)	2089
BtsIMutI	CAGTG	8 cut(s)	373, 658, 922, 1182, 1775, 2089, 2255, 2873
BveI	ACCTGC	1 cut(s)	25
CaiI	CAGNNNCTG	1 cut(s)	2012
CfoI	GCGC	2 cut(s)	7, 1062
Cfr10I	RCCGGY	2 cut(s)	2569, 2903
Cfr13I	GGNCC	3 cut(s)	19, 243, 992
Cfr42I	CCGCGG	1 cut(s)	55
CseI	GACGC	1 cut(s)	214
Csp6I	GTAC	6 cut(s)	602, 1238, 1425, 1571, 1960, 2591
CviQI	GTAC	6 cut(s)	602, 1238, 1425, 1571, 1960, 2591
DdeI	CTNAG	6 cut(s)	14, 239, 360, 914, 2215, 2957
DinI	GGCGCC	1 cut(s)	6
DrdI	GACNNNNNNGTC	1 cut(s)	714
DseDI	GACNNNNNNGTC	1 cut(s)	714
EaeI	YGGCCR	1 cut(s)	49
EagI	CGGCCG	1 cut(s)	49
Ecl136II	GAGCTC	2 cut(s)	215, 1946
EclXI	CGGCCG	1 cut(s)	49
Eco130I	CCWWGG	5 cut(s)	780, 1125, 1993, 2820, 2894
Eco147I	AGGCCT	1 cut(s)	90
Eco24I	GRGCYC	5 cut(s)	217, 366, 1087, 1948, 2187
Eco31I	GGTCTC	1 cut(s)	1200
Eco32I	GATATC	1 cut(s)	313
Eco47I	GGWCC	1 cut(s)	19
Eco47III	AGCGCT	1 cut(s)	1061
Eco52I	CGGCCG	1 cut(s)	49
Eco53kI	GAGCTC	2 cut(s)	215, 1946
Eco57I	CTGAAG	2 cut(s)	245, 780
Eco81I	CCTNAGG	1 cut(s)	2957
Eco88I	CYCGRG	2 cut(s)	92, 2776
EcoICRI	GAGCTC	2 cut(s)	215, 1946
EcoO109I	RGGNCCY	2 cut(s)	19, 243
EcoRI	GAATTC	1 cut(s)	124
EcoRII	CCWGG	5 cut(s)	475, 906, 1396, 1420, 2233
EcoRV	GATATC	1 cut(s)	313
EcoT14I	CCWWGG	5 cut(s)	780, 1125, 1993, 2820, 2894
EcoT22I	ATGCAT	1 cut(s)	2915
EcoT38I	GRGCYC	5 cut(s)	217, 366, 1087, 1948, 2187
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	5 cut(s)	780, 1125, 1993, 2820, 2894
FalI	AAGNNNNNCTT	2 cut(s)	838, 870
FaqI	GGGAC	3 cut(s)	32, 2472, 2710
FauNDI	CATATG	1 cut(s)	2386
FbaI	TGATCA	1 cut(s)	2757
FblI	GTMKAC	1 cut(s)	1521
FokI	GGATG	6 cut(s)	160, 190, 317, 1124, 2211, 2231
FriOI	GRGCYC	5 cut(s)	217, 366, 1087, 1948, 2187
FspBI	CTAG	6 cut(s)	515, 774, 804, 2079, 2096, 2660
GlaI	GCGC	2 cut(s)	6, 1061
GsaI	CCCAGC	1 cut(s)	339
GsuI	CTGGAG	6 cut(s)	8, 203, 1003, 1299, 1419, 1764
HaeII	RGCGCY	2 cut(s)	8, 1063
HaeIII	GGCC	6 cut(s)	51, 90, 245, 994, 1139, 2825
HapII	CCGG	4 cut(s)	48, 174, 2570, 2904
HgaI	GACGC	1 cut(s)	214
HhaI	GCGC	2 cut(s)	7, 1062
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	2 cut(s)	5, 1060
HinP1I	GCGC	2 cut(s)	5, 1060
HincII	GTYRAC	2 cut(s)	1522, 1719
HindII	GTYRAC	2 cut(s)	1522, 1719
HinfI	GANTC	6 cut(s)	10, 113, 265, 2310, 2640, 2648
HpaII	CCGG	4 cut(s)	48, 174, 2570, 2904
HphI	GGTGA	6 cut(s)	95, 715, 1019, 1042, 1543, 2645
Hpy166II	GTNNAC	6 cut(s)	445, 1163, 1522, 1719, 1850, 2087
Hpy8I	GTNNAC	6 cut(s)	445, 1163, 1522, 1719, 1850, 2087
Hpy99I	CGWCG	2 cut(s)	71, 74
HpyAV	CCTTC	7 cut(s)	80, 137, 449, 1574, 2157, 2384, 2880
HpyCH4III	ACNGT	6 cut(s)	157, 541, 1600, 1699, 1770, 2008
HpyCH4IV	ACGT	8 cut(s)	99, 182, 276, 1406, 1526, 1569, 1962, 2593
HpyF3I	CTNAG	6 cut(s)	14, 239, 360, 914, 2215, 2957
HpySE526I	ACGT	8 cut(s)	99, 182, 276, 1406, 1526, 1569, 1962, 2593
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	2 cut(s)	5, 1060
KasI	GGCGCC	1 cut(s)	4
KroI	GCCGGC	1 cut(s)	2569
KroNI	GCCGGC	1 cut(s)	2571
Ksp22I	TGATCA	1 cut(s)	2757
KspI	CCGCGG	1 cut(s)	55
LmnI	GCTCC	8 cut(s)	212, 1654, 1745, 1754, 1779, 2182, 2948, 2958
Lsp1109I	GCAGC	9 cut(s)	175, 397, 446, 1063, 1085, 2428, 2650, 2729, 2874
MaeI	CTAG	6 cut(s)	515, 774, 804, 2079, 2096, 2660
MaeII	ACGT	8 cut(s)	99, 182, 276, 1406, 1526, 1569, 1962, 2593
MaeIII	GTNAC	7 cut(s)	169, 637, 766, 1486, 1576, 2008, 2491
MbiI	CCGCTC	1 cut(s)	2558
MboII	GAAGA	7 cut(s)	152, 320, 341, 587, 1409, 2366, 2762
MfeI	CAATTG	2 cut(s)	324, 2709
MflI	RGATCY	2 cut(s)	776, 1589
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	19, 2642
MmeI	TCCRAC	4 cut(s)	1223, 1342, 1567, 2345
Mph1103I	ATGCAT	1 cut(s)	2915
MroNI	GCCGGC	1 cut(s)	2569
MroXI	GAANNNNTTC	2 cut(s)	459, 1096
MseI	TTAA	6 cut(s)	1338, 1409, 1617, 1956, 2055, 2544
MslI	CAYNNNNRTG	3 cut(s)	1529, 2033, 2918
MspA1I	CMGCKG	4 cut(s)	54, 1076, 1874, 2720
MspCI	CTTAAG	1 cut(s)	2054
MspI	CCGG	4 cut(s)	48, 174, 2570, 2904
MspR9I	CCNGG	6 cut(s)	175, 477, 908, 1398, 1422, 2235
MunI	CAATTG	2 cut(s)	324, 2709
MvaI	CCWGG	5 cut(s)	477, 908, 1398, 1422, 2235
MvnI	CGCG	2 cut(s)	54, 2043
NaeI	GCCGGC	1 cut(s)	2571
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	1 cut(s)	175
NcoI	CCATGG	2 cut(s)	2820, 2894
NdeI	CATATG	1 cut(s)	2386
NgoMIV	GCCGGC	1 cut(s)	2569
NheI	GCTAGC	2 cut(s)	803, 2078
NlaIV	GGNNCC	7 cut(s)	6, 20, 1561, 1747, 1775, 1984, 2184
NmeAIII	GCCGAG	1 cut(s)	33
NmuCI	GTSAC	1 cut(s)	766
NsiI	ATGCAT	1 cut(s)	2915
NspI	RCATGY	3 cut(s)	432, 1842, 2913
NspV	TTCGAA	1 cut(s)	999
OliI	CACNNNNGTG	1 cut(s)	1529
PaeI	GCATGC	2 cut(s)	1842, 2913
PaeR7I	CTCGAG	2 cut(s)	92, 2776
PceI	AGGCCT	1 cut(s)	90
PciI	ACATGT	1 cut(s)	428
PdiI	GCCGGC	1 cut(s)	2571
PdmI	GAANNNNTTC	2 cut(s)	459, 1096
PfeI	GAWTC	4 cut(s)	113, 265, 2310, 2640
PflFI	GACNNNGTC	1 cut(s)	560
PleI	GAGTC	2 cut(s)	18, 2642
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	2 cut(s)	18, 2642
Ppu21I	YACGTR	2 cut(s)	1570, 1963
PpuMI	RGGWCCY	1 cut(s)	19
PscI	ACATGT	1 cut(s)	428
PshBI	ATTAAT	1 cut(s)	1617
Psp124BI	GAGCTC	2 cut(s)	217, 1948
Psp5II	RGGWCCY	1 cut(s)	19
Psp6I	CCWGG	5 cut(s)	475, 906, 1396, 1420, 2233
PspFI	CCCAGC	1 cut(s)	335
PspGI	CCWGG	5 cut(s)	475, 906, 1396, 1420, 2233
PspN4I	GGNNCC	7 cut(s)	6, 20, 1561, 1747, 1775, 1984, 2184
PspPI	GGNCC	3 cut(s)	19, 243, 992
PspPPI	RGGWCCY	1 cut(s)	19
PspXI	VCTCGAGB	1 cut(s)	92
PstI	CTGCAG	1 cut(s)	2074
PstNI	CAGNNNCTG	1 cut(s)	2012
PsuI	RGATCY	2 cut(s)	776, 1589
PsyI	GACNNNGTC	1 cut(s)	560
PvuII	CAGCTG	3 cut(s)	1076, 1874, 2720
RsaI	GTAC	6 cut(s)	603, 1239, 1426, 1572, 1961, 2592
RsaNI	GTAC	6 cut(s)	602, 1238, 1425, 1571, 1960, 2591
RseI	CAYNNNNRTG	3 cut(s)	1529, 2033, 2918
SacI	GAGCTC	2 cut(s)	217, 1948
SacII	CCGCGG	1 cut(s)	55
SalI	GTCGAC	1 cut(s)	1520
SaqAI	TTAA	6 cut(s)	1338, 1409, 1617, 1956, 2055, 2544
Sau96I	GGNCC	3 cut(s)	19, 243, 992
SchI	GAGTC	2 cut(s)	19, 2642
ScrFI	CCNGG	6 cut(s)	175, 477, 908, 1398, 1422, 2235
SfcI	CTRYAG	5 cut(s)	492, 1320, 1698, 2004, 2070
SfoI	GGCGCC	1 cut(s)	6
Sfr274I	CTCGAG	2 cut(s)	92, 2776
Sfr303I	CCGCGG	1 cut(s)	55
SfuI	TTCGAA	1 cut(s)	999
SgrBI	CCGCGG	1 cut(s)	55
SinI	GGWCC	1 cut(s)	19
SlaI	CTCGAG	2 cut(s)	92, 2776
SmiMI	CAYNNNNRTG	3 cut(s)	1529, 2033, 2918
SmlI	CTYRAG	3 cut(s)	92, 2054, 2776
SmoI	CTYRAG	3 cut(s)	92, 2054, 2776
SphI	GCATGC	2 cut(s)	1842, 2913
SseBI	AGGCCT	1 cut(s)	90
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	6 cut(s)	515, 774, 804, 2079, 2096, 2660
SstI	GAGCTC	2 cut(s)	217, 1948
StuI	AGGCCT	1 cut(s)	90
StyD4I	CCNGG	6 cut(s)	173, 475, 906, 1396, 1420, 2233
StyI	CCWWGG	5 cut(s)	780, 1125, 1993, 2820, 2894
TaaI	ACNGT	6 cut(s)	157, 541, 1600, 1699, 1770, 2008
TaiI	ACGT	8 cut(s)	102, 185, 279, 1409, 1529, 1572, 1965, 2596
TaqI	TCGA	8 cut(s)	93, 413, 937, 999, 1521, 1626, 2313, 2777
TatI	WGTACW	2 cut(s)	601, 1237
TfiI	GAWTC	4 cut(s)	113, 265, 2310, 2640
Tru1I	TTAA	6 cut(s)	1338, 1409, 1617, 1956, 2055, 2544
Tru9I	TTAA	6 cut(s)	1338, 1409, 1617, 1956, 2055, 2544
TscAI	CASTG	8 cut(s)	373, 658, 922, 1189, 1775, 2089, 2255, 2880
TseFI	GTSAC	1 cut(s)	766
TseI	GCWGC	9 cut(s)	188, 385, 434, 1073, 1076, 2441, 2663, 2717, 2887
Tsp45I	GTSAC	1 cut(s)	766
TspDTI	ATGAA	6 cut(s)	321, 885, 886, 960, 2403, 2586
TspGWI	ACGGA	2 cut(s)	238, 390
TspRI	CASTG	8 cut(s)	373, 658, 922, 1189, 1775, 2089, 2255, 2880
Tth111I	GACNNNGTC	1 cut(s)	560
Vha464I	CTTAAG	1 cut(s)	2054
VneI	GTGCAC	2 cut(s)	1161, 1848
VpaK11BI	GGWCC	1 cut(s)	19
VspI	ATTAAT	1 cut(s)	1617
XapI	RAATTY	3 cut(s)	124, 1549, 1661
XbaI	TCTAGA	1 cut(s)	773
XceI	RCATGY	3 cut(s)	432, 1842, 2913
XcmI	CCANNNNNNNNNTGG	2 cut(s)	227, 1825
XhoI	CTCGAG	2 cut(s)	92, 2776
XmiI	GTMKAC	1 cut(s)	1521
XmnI	GAANNNNTTC	2 cut(s)	459, 1096
XspI	CTAG	6 cut(s)	515, 774, 804, 2079, 2096, 2660
Zsp2I	ATGCAT	1 cut(s)	2915

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.