Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0009249

Physical Location & Seq

Genomic Coordinates Reverse (-)

37945912 .. 37952509

Gene Structure 6598 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1247900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3009 bp

ATGGCGCCGAGTCCCAGAGACCTCCAGCTTTCCCAAATCTCCGCCCGAGCTCCGACGTCGATGGTTGCCGCCGGCGACTTCGGCGATTTGGAGGACGTGAGGCTCCTGGATTCGTATGAGAATTCGGAGGGAGTTGAAGAGGGGATGAAGAGGGTTCAAGTCAGAGTCTCTGGCATGACGTGTGCTGCTTGCTCGAATTCTGTCGAAGGTGCTCTGCAGAGCGTCAAAGGGGTTCTCACGGCCTCCGTGGCGTTGCTCCAGAGTAGGGCCGATGTGGTTTTTGATCCGAGATTGGTCAAGGATGAAGACATCAAGAATGCAATTGAAGATGCCGGGTTTGAGGCAGAGGTTATACCTGAGCCCAGTGCAAGTGGAACAAAGCCACATGGTACCCTGTCTGGTCAGTTCTCAATAGGAGGTATGACCTGTGCTGCCTGTGTGAACTCTGTTGAAGGTATTCTAAAAGGGCTTCCTGGGGTCAAAAGAGCTGTAGTAGCCCTGGCAACTTCACTAGGGGAAGTGGAGTATGATCCTCTGGTAATTAGTAAAGATGAGATAGTGAATGCCATTGAAGATGCTGGTTTTGATGCATCACTTGTACAGAGTAGTCAACAGGATAAAATCGTACTTGGAGTTGCTGGCGTATTCAGTGAGATGGATGCACAGACTTTAGAAGCCATAATTAGCAATTTGAAAGGGGTGAGACATTTTCGTGTTGACAGGATTTCAAGAGAACTTGAAATCCTCTTTGATCCTGAAGTTGTCACTTCTAGATCCTTGGTGGATGAGATTCAGGAAGCAAGCAATGAGAAATTTAAACTACAAGTGGCAAACCCTTATACAAGAATGACTTCTAAAGATATCGATGAAGCCTCAAATATGTTTCGGCTGTTTCTTTCCAGTTTGCTTCTCAGTATTCCTATCTTTTTCATTCGAGTAGTTTGTCCTCATATCCCGCTTCTTTATTCGTTACTGCTCTGGAAATGTGGGCCCTTTGAAATGGGTGATTGGTTGAAGTGGGCATTGGTCAGTGTTGTTCAGTTTGTTATTGGGAAGCGCTTCTACATTGCAGCTGCCAGAGCCTTACGAAATGGTTCAACTAACATGGATGTTTTGGTCGCATTGGGAACTTCGGCCTCTTACTTCTATTCTGTATGCGCACTTCTATATGGTGCAGTCACTGGGTTCTGGTCTCCAACGTACTTTGAAACAAGTGCCATGCTAATAACATTTGTACTTTTGGGGAAATATCTGGAGTGCCTTGCAAAAGGAAAAACATCAGATGCCATCAAGAAGTTGATAGAACTTGCTCCAGCTACAGCATTGTTAATTGTCAAAGATAAAGGTGGGAAAGTCATTGGAGAAAGGGAAATTGATGCCTTGCTGATTCAGCCCAGGGATGTGTTAAAGGTTCTTCCTGGTACCAAGGTTCCTGCTGATGGTATGGTTGTGTGGGGTTCAAGTTATGTCAATGAGAGTATGGTGACCGGTGAAGCTATACCTGTTTCAAAGGAAGTAAATTCATTAGTTATCGGAGGAACGATAAATTTACATGGAGCTCTTCATATACAAGTTACAAAAGTTGGATCCGATACAGTTTTAAGTCAGATAATTAATTTGGTCGAGACTGCTCAGATGTCCAAAGCTCCTATTCAGAAATTTGCTGATTTTATTGCTAGCATTTTCGTCCCTACTGTAGTTGCCTTGGCATTGTTGACATTGTTGGGTTGGTATATTGCTGGAGCCTTTGGAGCTTACCCAGAAAAGTGGCTCCCTGAAAACGGAAATCACTTTGTATTTGCCCTTATGTTTTCCATATCGGTTGTGGTAATTGCATGTCCTTGTGCACTGGGCTTGGCAACACCAACTGCTGTCATGGTTGCGACGGGGGTTGGGGCTAATAATGGTGTGCTGATAAAAGGAGGAGATGCTTTGGAAAGAGCTCAGAAGATCAAGTATGTGATATTTGATAAAACTGGTACCCTGACCCAGGGAAAAGCGTCAGTTACTACTGTAAAAGTTTTCACGGGAATGGATCGTGGAGAATTTCTTAAATTGGTGGCTTCTGCAGAGGCTAGCAGTGAACACCCATTGGCAAAAGCAATAGTGGAATATGCACGCCATTTTCACTTTTTTGATGAGCCTTCTGTAACTAACGATGAACCGAACAAGAGCAAAGACACCACACTTTCTGGATGGCTTTTTGATGCCTCAGAGTTCTCTGCTTTGCCCGGAAGAGGTATACAGTGCTTTATAGATGGAAAACTTGTTCTGGTCGGTAATAGGAAGCTGATGACTGAAAGTGGAATTGACATTCCTACACATGTAGAGAACTTTGTAGTAGAGCTGGAAGAAAGTGCAAAGACTGGCATACTTGTTGCATATGAAGGCAATTTAATTGGTGTTTTAGGGGTTGCGGACCCTCTAAAAAGAGAAGCTGCCATAGTGATAGAAGGACTACGTAAGATGGGTGTCATACCAGTCATGGTTACTGGGGATAATCGGAGGACAGCACAAGCTGTTGCTAAGGAGGTTGGCATTCAAGACGTGAGGGCAGAGGTAATGCCGGCTGGAAAAGCTGATGTCGTTTGTTCATTCCAGAAAGATGGAAGTATAGTTGCCATGGTTGGTGATGGAATCAATGACTCTCCTGCCCTAGCTGCTTCTGATGTTGGTATGGCAATCGGGGCAGGGACGGATATTGCAATTGAAGCAGCTGACTACGTGCTGATGAGAAATAACTTGGAAGACGTAATCACAGCCATTGATCTCTCAAGGAAAACCTTTACTCGTATTCGATTGAATTACGTGTTTGCCATGGCGTACAATGTGATCGCAATACCTATTGCCGCAGGGGTCTTCTTCCCATCGCTGGGGATCATGTTGCCGCCATGGGCAGCGGGTGCATGCATGGCTATGTCATCTGTTAGTGTTGTATGCTCTTCTTTGCTCCTTAGGAGGTACAAAAAACCCAGACTTACCGCCATACTGGAAATAGTTGTAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0012505 GO:0016020 GO:0023052 GO:0031410 GO:0031982 GO:0031984 GO:0032870 GO:0035556 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071369 GO:0071495 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1003

Amino Acids

107.32

Weight (kDa)

5.24

Isoelectric Point (pI)

30.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	54 - 114	1.1e-14	Heavy-metal-associated domain
HMA	PF00403	136 - 196	1.3e-14	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	437 - 542	1.4e-22	P-type ATPase actuator domain
Hydrolase	PF00702	651 - 889	2.6e-30	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44790
fragaria_vesca	FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710 FvH4_3g12710
malus_domestica	MD05G1247900.v1.1 MD10G1228800.v1.1
prunus_persica	Prupe.4G115900_v2.0.a1 Prupe.4G115900_v2.0.a1
pyrus_communis	pycom05g22420 pycom10g19240
rosa_chinensis	RchiOBHm_Chr5g0020701
rosa_laevigata	RLG00000032554
rosa_multiflora	Rmu_sc0005607.1_g000032
rosa_roxburghii	Rroxscaffold_1G00057600
rosa_rugosa	Rorug05G0062500
rosa_samantha	Rh5AG152200 Rh5BG151900 Rh5CG163300 Rh5DG151400
rosa_wichuraiana	Rw5G013490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	59
Acc16I	TGCGCA	1 cut(s)	1159
Acc65I	GGTACC	3 cut(s)	389, 1421, 1979
AccB1I	GGYRCC	4 cut(s)	4, 389, 1421, 1979
AccI	GTMKAC	1 cut(s)	2242
AciI	CCGC	8 cut(s)	42, 69, 956, 2417, 2850, 2888, 2900, 2982
AclWI	GGATC	8 cut(s)	278, 524, 746, 768, 1581, 1594, 2043, 2885
AcsI	RAATTY	7 cut(s)	121, 196, 812, 1519, 1546, 1658, 2045
AcuI	CTGAAG	1 cut(s)	777
AcyI	GRCGYC	2 cut(s)	5, 56
AfaI	GTAC	9 cut(s)	391, 600, 627, 1202, 1236, 1423, 1981, 2825, 2963
AfeI	AGCGCT	1 cut(s)	1058
AfiI	CCNNNNNNNGG	7 cut(s)	265, 1439, 1781, 1990, 2237, 2872, 2873
AflIII	ACRYGT	3 cut(s)	179, 2323, 2808
AgeI	ACCGGT	1 cut(s)	1487
AjiI	CACGTC	3 cut(s)	97, 180, 2548
AjnI	CCWGG	6 cut(s)	105, 472, 498, 1394, 1417, 1989
AjuI	GAANNNNNNNTTGG	2 cut(s)	1007, 1039
AloI	GAACNNNNNNTCC	4 cut(s)	726, 758, 2253, 2285
Alw21I	GWGCWC	5 cut(s)	52, 214, 1561, 1849, 1945
Alw26I	GTCTC	5 cut(s)	12, 172, 697, 1197, 1619
Alw44I	GTGCAC	1 cut(s)	1845
AlwI	GGATC	8 cut(s)	278, 524, 746, 768, 1581, 1594, 2043, 2885
AlwNI	CAGNNNCTG	1 cut(s)	1181
Ama87I	CYCGRG	1 cut(s)	45
Aor51HI	AGCGCT	1 cut(s)	1058
AoxI	GGCC	4 cut(s)	240, 267, 989, 1134
ApaI	GGGCCC	1 cut(s)	993
ApaLI	GTGCAC	1 cut(s)	1845
ApeKI	GCWGC	8 cut(s)	185, 431, 1070, 1073, 2438, 2660, 2714, 2897
ApoI	RAATTY	7 cut(s)	121, 196, 812, 1519, 1546, 1658, 2045
AseI	ATTAAT	1 cut(s)	1614
AsiGI	ACCGGT	1 cut(s)	1487
Asp700I	GAANNNNTTC	4 cut(s)	456, 850, 1058, 1093
Asp718I	GGTACC	3 cut(s)	389, 1421, 1979
AspLEI	GCGC	3 cut(s)	7, 1059, 1160
AspS9I	GGNCC	4 cut(s)	267, 989, 990, 2419
AsuC2I	CCSGG	2 cut(s)	334, 2232
AsuHPI	GGTGA	5 cut(s)	712, 1016, 1495, 1502, 2642
AsuNHI	GCTAGC	2 cut(s)	1676, 2075
AvaI	CYCGRG	1 cut(s)	45
AvaII	GGWCC	1 cut(s)	2419
AxyI	CCTNAGG	1 cut(s)	2954
BaeGI	GKGCMC	2 cut(s)	993, 1849
BaeI	ACNNNNGTAYC	2 cut(s)	1413, 1446
BamHI	GGATCC	1 cut(s)	1586
BanI	GGYRCC	4 cut(s)	4, 389, 1421, 1979
BanII	GRGCYC	5 cut(s)	52, 363, 993, 1561, 1945
BarI	GAAGNNNNNNTAC	4 cut(s)	1046, 1078, 1146, 1178
BbsI	GAAGAC	3 cut(s)	312, 2754, 2851
Bbv12I	GWGCWC	5 cut(s)	52, 214, 1561, 1849, 1945
BbvI	GCAGC	8 cut(s)	172, 418, 1060, 1082, 2425, 2647, 2726, 2909
BceAI	ACGGC	1 cut(s)	255
BciT130I	CCWGG	6 cut(s)	107, 474, 500, 1396, 1419, 1991
BcnI	CCSGG	2 cut(s)	334, 2232
BcoDI	GTCTC	5 cut(s)	12, 172, 697, 1197, 1619
BfaI	CTAG	5 cut(s)	512, 771, 1677, 2076, 2657
BfmI	CTRYAG	5 cut(s)	215, 489, 1317, 1695, 2067
BfoI	RGCGCY	2 cut(s)	8, 1060
BglI	GCCNNNNNGGC	1 cut(s)	248
Bme1390I	CCNGG	8 cut(s)	107, 334, 474, 500, 1396, 1419, 1991, 2232
Bme18I	GGWCC	1 cut(s)	2419
BmeT110I	CYCGRG	1 cut(s)	45
BmgBI	CACGTC	3 cut(s)	97, 180, 2548
BmgT120I	GGNCC	4 cut(s)	267, 989, 990, 2419
BmrFI	CCNGG	8 cut(s)	107, 334, 474, 500, 1396, 1419, 1991, 2232
BmrI	ACTGGG	4 cut(s)	357, 1191, 1859, 2502
BmsI	GCATC	9 cut(s)	319, 565, 577, 599, 649, 1273, 1366, 1918, 2197
BmtI	GCTAGC	2 cut(s)	1680, 2079
BmuI	ACTGGG	4 cut(s)	357, 1191, 1859, 2502
BpiI	GAAGAC	3 cut(s)	312, 2754, 2851
BpmI	CTGGAG	5 cut(s)	8, 242, 1274, 1296, 1761
Bpu10I	CCTNAGC	2 cut(s)	357, 2526
BpuEI	CTTGAG	1 cut(s)	2758
BpuMI	CCSGG	2 cut(s)	334, 2232
Bsa29I	ATCGAT	1 cut(s)	864
BsaAI	YACGTR	3 cut(s)	2462, 2725, 2809
BsaHI	GRCGYC	2 cut(s)	5, 56
BsaI	GGTCTC	2 cut(s)	12, 1197
BsaWI	WCCGGW	1 cut(s)	1487
Bsc4I	CCNNNNNNNGG	7 cut(s)	265, 1439, 1781, 1990, 2237, 2872, 2873
Bse118I	RCCGGY	3 cut(s)	71, 1487, 2566
Bse1I	ACTGG	9 cut(s)	363, 900, 1186, 1854, 1981, 2371, 2480, 2497, 2994
Bse21I	CCTNAGG	1 cut(s)	2954
Bse3DI	GCAATG	2 cut(s)	811, 1065
BseBI	CCWGG	6 cut(s)	107, 474, 500, 1396, 1419, 1991
BseCI	ATCGAT	1 cut(s)	864
BseGI	GGATG	7 cut(s)	150, 307, 664, 790, 1114, 1405, 2201
BseLI	CCNNNNNNNGG	7 cut(s)	265, 1439, 1781, 1990, 2237, 2872, 2873
BseMI	GCAATG	2 cut(s)	811, 1065
BseMII	CTCAG	5 cut(s)	348, 925, 1646, 1958, 2226
BseNI	ACTGG	9 cut(s)	363, 900, 1186, 1854, 1981, 2371, 2480, 2497, 2994
BseRI	GAGGAG	1 cut(s)	1938
BseSI	GKGCMC	2 cut(s)	993, 1849
BseXI	GCAGC	8 cut(s)	172, 418, 1060, 1082, 2425, 2647, 2726, 2909
BseYI	CCCAGC	1 cut(s)	2872
BsgI	GTGCAG	1 cut(s)	1194
BshFI	GGCC	4 cut(s)	242, 269, 991, 1136
BshNI	GGYRCC	4 cut(s)	4, 389, 1421, 1979
BshTI	ACCGGT	1 cut(s)	1487
BshVI	ATCGAT	1 cut(s)	864
BsiHKAI	GWGCWC	5 cut(s)	52, 214, 1561, 1849, 1945
BsiHKCI	CYCGRG	1 cut(s)	45
BsiSI	CCGG	5 cut(s)	72, 333, 1488, 2232, 2567
BslFI	GGGAC	2 cut(s)	1673, 2707
BslI	CCNNNNNNNGG	7 cut(s)	265, 1439, 1781, 1990, 2237, 2872, 2873
BsmAI	GTCTC	5 cut(s)	12, 172, 697, 1197, 1619
BsmFI	GGGAC	2 cut(s)	1673, 2707
BsmI	GAATGC	3 cut(s)	322, 568, 2538
BsnI	GGCC	4 cut(s)	242, 269, 991, 1136
Bso31I	GGTCTC	2 cut(s)	12, 1197
BsoBI	CYCGRG	1 cut(s)	45
Bsp120I	GGGCCC	1 cut(s)	989
Bsp1286I	GDGCHC	7 cut(s)	52, 214, 363, 993, 1561, 1849, 1945
Bsp1407I	TGTACA	1 cut(s)	598
Bsp19I	CCATGG	3 cut(s)	2622, 2817, 2891
BspACI	CCGC	8 cut(s)	42, 69, 956, 2417, 2850, 2888, 2900, 2982
BspANI	GGCC	4 cut(s)	242, 269, 991, 1136
BspCNI	CTCAG	5 cut(s)	349, 924, 1645, 1957, 2225
BspDI	ATCGAT	1 cut(s)	864
BspMAI	CTGCAG	2 cut(s)	219, 2071
BspOI	GCTAGC	2 cut(s)	1680, 2079
BspPI	GGATC	8 cut(s)	278, 524, 746, 768, 1581, 1594, 2043, 2885
BspQI	GCTCTTC	2 cut(s)	1566, 2947
BspT107I	GGYRCC	4 cut(s)	4, 389, 1421, 1979
BspTNI	GGTCTC	2 cut(s)	12, 1197
BsrDI	GCAATG	2 cut(s)	811, 1065
BsrFI	RCCGGY	3 cut(s)	71, 1487, 2566
BsrGI	TGTACA	1 cut(s)	598
BsrI	ACTGG	9 cut(s)	363, 900, 1186, 1854, 1981, 2371, 2480, 2497, 2994
BssAI	RCCGGY	3 cut(s)	71, 1487, 2566
BssNAI	GTATAC	1 cut(s)	2243
BssNI	GRCGYC	2 cut(s)	5, 56
BssT1I	CCWWGG	6 cut(s)	777, 1425, 1704, 2622, 2817, 2891
Bst1107I	GTATAC	1 cut(s)	2243
Bst2UI	CCWGG	6 cut(s)	107, 474, 500, 1396, 1419, 1991
Bst4CI	ACNGT	4 cut(s)	1597, 1696, 2014, 2247
Bst6I	CTCTTC	5 cut(s)	132, 143, 1566, 2230, 2947
BstACI	GRCGYC	2 cut(s)	5, 56
BstAPI	GCANNNNNTGC	1 cut(s)	2903
BstAUI	TGTACA	1 cut(s)	598
BstBAI	YACGTR	3 cut(s)	2462, 2725, 2809
BstC8I	GCNNGC	9 cut(s)	73, 190, 640, 802, 1678, 2077, 2119, 2568, 2908
BstDEI	CTNAG	7 cut(s)	357, 911, 1632, 1944, 2212, 2526, 2954
BstDSI	CCRYGG	4 cut(s)	246, 2622, 2817, 2891
BstEII	GGTNACC	1 cut(s)	1483
BstENI	CCTNNNNNAGG	1 cut(s)	1988
BstF5I	GGATG	7 cut(s)	150, 307, 664, 790, 1114, 1405, 2201
BstH2I	RGCGCY	2 cut(s)	8, 1060
BstHHI	GCGC	3 cut(s)	7, 1059, 1160
BstMAI	GTCTC	5 cut(s)	12, 172, 697, 1197, 1619
BstNI	CCWGG	6 cut(s)	107, 474, 500, 1396, 1419, 1991
BstNSI	RCATGY	3 cut(s)	1839, 2327, 2910
BstPI	GGTNACC	1 cut(s)	1483
BstSCI	CCNGG	8 cut(s)	105, 332, 472, 498, 1394, 1417, 1989, 2230
BstSFI	CTRYAG	5 cut(s)	215, 489, 1317, 1695, 2067
BstSLI	GKGCMC	2 cut(s)	993, 1849
BstSNI	TACGTA	1 cut(s)	2462
BstV1I	GCAGC	8 cut(s)	172, 418, 1060, 1082, 2425, 2647, 2726, 2909
BstV2I	GAAGAC	3 cut(s)	312, 2754, 2851
BstX2I	RGATCY	2 cut(s)	773, 1586
BstXI	CCANNNNNNTGG	2 cut(s)	1767, 2606
BstYI	RGATCY	2 cut(s)	773, 1586
BstZ17I	GTATAC	1 cut(s)	2243
Bsu15I	ATCGAT	1 cut(s)	864
Bsu36I	CCTNAGG	1 cut(s)	2954
BsuRI	GGCC	4 cut(s)	242, 269, 991, 1136
BsuTUI	ATCGAT	1 cut(s)	864
BtgI	CCRYGG	4 cut(s)	246, 2622, 2817, 2891
BtgZI	GCGATG	1 cut(s)	2853
BtrI	CACGTC	3 cut(s)	97, 180, 2548
BtsCI	GGATG	7 cut(s)	150, 307, 664, 790, 1114, 1405, 2201
BtsI	GCAGTG	1 cut(s)	2086
BtsIMutI	CAGTG	7 cut(s)	370, 655, 1036, 1179, 1847, 2086, 2252
Cac8I	GCNNGC	9 cut(s)	73, 190, 640, 802, 1678, 2077, 2119, 2568, 2908
CaiI	CAGNNNCTG	1 cut(s)	1181
CfoI	GCGC	3 cut(s)	7, 1059, 1160
Cfr10I	RCCGGY	3 cut(s)	71, 1487, 2566
Cfr13I	GGNCC	4 cut(s)	267, 989, 990, 2419
ClaI	ATCGAT	1 cut(s)	864
CseI	GACGC	2 cut(s)	211, 1989
Csp6I	GTAC	9 cut(s)	390, 599, 626, 1201, 1235, 1422, 1980, 2824, 2962
CspAI	ACCGGT	1 cut(s)	1487
CviQI	GTAC	9 cut(s)	390, 599, 626, 1201, 1235, 1422, 1980, 2824, 2962
DdeI	CTNAG	7 cut(s)	357, 911, 1632, 1944, 2212, 2526, 2954
DinI	GGCGCC	1 cut(s)	6
DraI	TTTAAA	1 cut(s)	817
Eam1104I	CTCTTC	5 cut(s)	132, 143, 1566, 2230, 2947
EarI	CTCTTC	5 cut(s)	132, 143, 1566, 2230, 2947
EciI	GGCGGA	1 cut(s)	31
Ecl136II	GAGCTC	3 cut(s)	50, 1559, 1943
Eco105I	TACGTA	1 cut(s)	2462
Eco130I	CCWWGG	6 cut(s)	777, 1425, 1704, 2622, 2817, 2891
Eco24I	GRGCYC	5 cut(s)	52, 363, 993, 1561, 1945
Eco31I	GGTCTC	2 cut(s)	12, 1197
Eco32I	GATATC	1 cut(s)	862
Eco47I	GGWCC	1 cut(s)	2419
Eco47III	AGCGCT	1 cut(s)	1058
Eco53kI	GAGCTC	3 cut(s)	50, 1559, 1943
Eco57I	CTGAAG	1 cut(s)	777
Eco81I	CCTNAGG	1 cut(s)	2954
Eco88I	CYCGRG	1 cut(s)	45
Eco91I	GGTNACC	1 cut(s)	1483
EcoICRI	GAGCTC	3 cut(s)	50, 1559, 1943
EcoNI	CCTNNNNNAGG	1 cut(s)	1988
EcoO109I	RGGNCCY	1 cut(s)	990
EcoO65I	GGTNACC	1 cut(s)	1483
EcoRI	GAATTC	2 cut(s)	121, 196
EcoRII	CCWGG	6 cut(s)	105, 472, 498, 1394, 1417, 1989
EcoRV	GATATC	1 cut(s)	862
EcoT14I	CCWWGG	6 cut(s)	777, 1425, 1704, 2622, 2817, 2891
EcoT22I	ATGCAT	2 cut(s)	592, 2912
EcoT38I	GRGCYC	5 cut(s)	52, 363, 993, 1561, 1945
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	6 cut(s)	777, 1425, 1704, 2622, 2817, 2891
FalI	AAGNNNNNCTT	2 cut(s)	835, 867
FaqI	GGGAC	2 cut(s)	1673, 2707
FauI	CCCGC	2 cut(s)	963, 2893
FauNDI	CATATG	1 cut(s)	2383
FblI	GTMKAC	1 cut(s)	2242
FokI	GGATG	7 cut(s)	157, 314, 671, 797, 1121, 1412, 2208
FriOI	GRGCYC	5 cut(s)	52, 363, 993, 1561, 1945
FspAI	RTGCGCAY	1 cut(s)	1159
FspBI	CTAG	5 cut(s)	512, 771, 1677, 2076, 2657
FspI	TGCGCA	1 cut(s)	1159
GlaI	GCGC	3 cut(s)	6, 1058, 1159
GsaI	CCCAGC	1 cut(s)	2876
GsuI	CTGGAG	5 cut(s)	8, 242, 1274, 1296, 1761
HaeII	RGCGCY	2 cut(s)	8, 1060
HaeIII	GGCC	4 cut(s)	242, 269, 991, 1136
HapII	CCGG	5 cut(s)	72, 333, 1488, 2232, 2567
HgaI	GACGC	2 cut(s)	211, 1989
HhaI	GCGC	3 cut(s)	7, 1059, 1160
Hin1I	GRCGYC	2 cut(s)	5, 56
Hin6I	GCGC	3 cut(s)	5, 1057, 1158
HinP1I	GCGC	3 cut(s)	5, 1057, 1158
HincII	GTYRAC	3 cut(s)	611, 718, 1716
HindII	GTYRAC	3 cut(s)	611, 718, 1716
HinfI	GANTC	7 cut(s)	10, 110, 165, 790, 1387, 2637, 2645
HpaII	CCGG	5 cut(s)	72, 333, 1488, 2232, 2567
HphI	GGTGA	5 cut(s)	712, 1016, 1495, 1502, 2642
Hpy166II	GTNNAC	7 cut(s)	442, 611, 718, 1716, 1847, 2084, 2243
Hpy8I	GTNNAC	7 cut(s)	442, 611, 718, 1716, 1847, 2084, 2243
Hpy99I	CGWCG	3 cut(s)	58, 61, 1888
HpyAV	CCTTC	5 cut(s)	200, 446, 2154, 2381, 2447
HpyCH4III	ACNGT	4 cut(s)	1597, 1696, 2014, 2247
HpyCH4IV	ACGT	9 cut(s)	56, 96, 179, 1199, 2461, 2547, 2724, 2751, 2808
HpyF3I	CTNAG	7 cut(s)	357, 911, 1632, 1944, 2212, 2526, 2954
HpySE526I	ACGT	9 cut(s)	56, 96, 179, 1199, 2461, 2547, 2724, 2751, 2808
Hsp92I	GRCGYC	2 cut(s)	5, 56
HspAI	GCGC	3 cut(s)	5, 1057, 1158
KasI	GGCGCC	1 cut(s)	4
KpnI	GGTACC	3 cut(s)	393, 1425, 1983
KroI	GCCGGC	2 cut(s)	71, 2566
KroNI	GCCGGC	2 cut(s)	73, 2568
LguI	GCTCTTC	2 cut(s)	1566, 2947
Lsp1109I	GCAGC	8 cut(s)	172, 418, 1060, 1082, 2425, 2647, 2726, 2909
LweI	GCATC	9 cut(s)	319, 565, 577, 599, 649, 1273, 1366, 1918, 2197
MaeI	CTAG	5 cut(s)	512, 771, 1677, 2076, 2657
MaeII	ACGT	9 cut(s)	56, 96, 179, 1199, 2461, 2547, 2724, 2751, 2808
MaeIII	GTNAC	8 cut(s)	763, 969, 1177, 1483, 1573, 2005, 2149, 2488
MfeI	CAATTG	2 cut(s)	321, 2706
MflI	RGATCY	2 cut(s)	773, 1586
MhlI	GDGCHC	7 cut(s)	52, 214, 363, 993, 1561, 1849, 1945
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	19, 174, 2639
MmeI	TCCRAC	3 cut(s)	77, 1220, 1564
Mph1103I	ATGCAT	2 cut(s)	592, 2912
MreI	CGCCGGCG	1 cut(s)	71
MroNI	GCCGGC	2 cut(s)	71, 2566
MroXI	GAANNNNTTC	4 cut(s)	456, 850, 1058, 1093
MseI	TTAA	7 cut(s)	816, 1328, 1406, 1601, 1614, 2052, 2396
MslI	CAYNNNNRTG	3 cut(s)	711, 2030, 2915
MspA1I	CMGCKG	3 cut(s)	1073, 2717, 2900
MspI	CCGG	5 cut(s)	72, 333, 1488, 2232, 2567
MspR9I	CCNGG	8 cut(s)	107, 334, 474, 500, 1396, 1419, 1991, 2232
MunI	CAATTG	2 cut(s)	321, 2706
Mva1269I	GAATGC	3 cut(s)	322, 568, 2538
MvaI	CCWGG	6 cut(s)	107, 474, 500, 1396, 1419, 1991
NaeI	GCCGGC	2 cut(s)	73, 2568
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	2 cut(s)	334, 2232
NcoI	CCATGG	3 cut(s)	2622, 2817, 2891
NdeI	CATATG	1 cut(s)	2383
NgoMIV	GCCGGC	2 cut(s)	71, 2566
NheI	GCTAGC	2 cut(s)	1676, 2075
NmeAIII	GCCGAG	1 cut(s)	33
NmuCI	GTSAC	3 cut(s)	763, 1177, 1483
NsbI	TGCGCA	1 cut(s)	1159
NsiI	ATGCAT	2 cut(s)	592, 2912
NspI	RCATGY	3 cut(s)	1839, 2327, 2910
PaeI	GCATGC	1 cut(s)	2910
PasI	CCCWGGG	2 cut(s)	1395, 1990
PciI	ACATGT	1 cut(s)	2323
PciSI	GCTCTTC	2 cut(s)	1566, 2947
PctI	GAATGC	3 cut(s)	322, 568, 2538
PdiI	GCCGGC	2 cut(s)	73, 2568
PdmI	GAANNNNTTC	4 cut(s)	456, 850, 1058, 1093
PfeI	GAWTC	4 cut(s)	110, 790, 1387, 2637
PfoI	TCCNGGA	1 cut(s)	105
PinAI	ACCGGT	1 cut(s)	1487
PleI	GAGTC	3 cut(s)	18, 173, 2639
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	3 cut(s)	18, 173, 2639
Ppu21I	YACGTR	3 cut(s)	2462, 2725, 2809
PscI	ACATGT	1 cut(s)	2323
PshBI	ATTAAT	1 cut(s)	1614
Psp124BI	GAGCTC	3 cut(s)	52, 1561, 1945
Psp6I	CCWGG	6 cut(s)	105, 472, 498, 1394, 1417, 1989
PspEI	GGTNACC	1 cut(s)	1483
PspFI	CCCAGC	1 cut(s)	2872
PspGI	CCWGG	6 cut(s)	105, 472, 498, 1394, 1417, 1989
PspOMI	GGGCCC	1 cut(s)	989
PspPI	GGNCC	4 cut(s)	267, 989, 990, 2419
PstI	CTGCAG	2 cut(s)	219, 2071
PstNI	CAGNNNCTG	1 cut(s)	1181
PsuI	RGATCY	2 cut(s)	773, 1586
PvuII	CAGCTG	2 cut(s)	1073, 2717
RsaI	GTAC	9 cut(s)	391, 600, 627, 1202, 1236, 1423, 1981, 2825, 2963
RsaNI	GTAC	9 cut(s)	390, 599, 626, 1201, 1235, 1422, 1980, 2824, 2962
RseI	CAYNNNNRTG	3 cut(s)	711, 2030, 2915
SacI	GAGCTC	3 cut(s)	52, 1561, 1945
SapI	GCTCTTC	2 cut(s)	1566, 2947
SaqAI	TTAA	7 cut(s)	816, 1328, 1406, 1601, 1614, 2052, 2396
Sau96I	GGNCC	4 cut(s)	267, 989, 990, 2419
SchI	GAGTC	3 cut(s)	19, 174, 2639
ScrFI	CCNGG	8 cut(s)	107, 334, 474, 500, 1396, 1419, 1991, 2232
SduI	GDGCHC	7 cut(s)	52, 214, 363, 993, 1561, 1849, 1945
SfaNI	GCATC	9 cut(s)	319, 565, 577, 599, 649, 1273, 1366, 1918, 2197
SfcI	CTRYAG	5 cut(s)	215, 489, 1317, 1695, 2067
SfoI	GGCGCC	1 cut(s)	6
SgrAI	CRCCGGYG	1 cut(s)	71
SinI	GGWCC	1 cut(s)	2419
SmiMI	CAYNNNNRTG	3 cut(s)	711, 2030, 2915
SmlI	CTYRAG	1 cut(s)	2773
SmoI	CTYRAG	1 cut(s)	2773
SnaBI	TACGTA	1 cut(s)	2462
SphI	GCATGC	1 cut(s)	2910
SsiI	CCGC	8 cut(s)	42, 69, 956, 2417, 2850, 2888, 2900, 2982
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	5 cut(s)	512, 771, 1677, 2076, 2657
SstI	GAGCTC	3 cut(s)	52, 1561, 1945
StyD4I	CCNGG	8 cut(s)	105, 332, 472, 498, 1394, 1417, 1989, 2230
StyI	CCWWGG	6 cut(s)	777, 1425, 1704, 2622, 2817, 2891
TaaI	ACNGT	4 cut(s)	1597, 1696, 2014, 2247
TaiI	ACGT	9 cut(s)	59, 99, 182, 1202, 2464, 2550, 2727, 2754, 2811
TaqI	TCGA	7 cut(s)	59, 194, 204, 864, 934, 1623, 2797
TatI	WGTACW	2 cut(s)	598, 1234
TauI	GCSGC	3 cut(s)	71, 2852, 2890
TfiI	GAWTC	4 cut(s)	110, 790, 1387, 2637
Tru1I	TTAA	7 cut(s)	816, 1328, 1406, 1601, 1614, 2052, 2396
Tru9I	TTAA	7 cut(s)	816, 1328, 1406, 1601, 1614, 2052, 2396
TscAI	CASTG	7 cut(s)	370, 655, 1036, 1186, 1854, 2086, 2252
TseFI	GTSAC	3 cut(s)	763, 1177, 1483
TseI	GCWGC	8 cut(s)	185, 431, 1070, 1073, 2438, 2660, 2714, 2897
Tsp45I	GTSAC	3 cut(s)	763, 1177, 1483
TspDTI	ATGAA	9 cut(s)	161, 318, 882, 919, 1512, 1553, 2175, 2400, 2583
TspGWI	ACGGA	3 cut(s)	235, 1797, 2711
TspRI	CASTG	7 cut(s)	370, 655, 1036, 1186, 1854, 2086, 2252
VneI	GTGCAC	1 cut(s)	1845
VpaK11BI	GGWCC	1 cut(s)	2419
VspI	ATTAAT	1 cut(s)	1614
XagI	CCTNNNNNAGG	1 cut(s)	1988
XapI	RAATTY	7 cut(s)	121, 196, 812, 1519, 1546, 1658, 2045
XbaI	TCTAGA	1 cut(s)	770
XceI	RCATGY	3 cut(s)	1839, 2327, 2910
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1822
XmiI	GTMKAC	1 cut(s)	2242
XmnI	GAANNNNTTC	4 cut(s)	456, 850, 1058, 1093
XspI	CTAG	5 cut(s)	512, 771, 1677, 2076, 2657
ZraI	GACGTC	1 cut(s)	57
Zsp2I	ATGCAT	2 cut(s)	592, 2912

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.