AT5G45950

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016036
Physical Location & Seq
Forward (+)
18634041 .. 18636720
2680 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45950.1

Sequence Viewer

Length: 1074 bp
ATGTTGCTTGTTGCGTTTGTGACTCTTTTGGTAGCAGTAGCTTTGCAGCCACTACCGTCAGTGTTGTCTTTGGACGTTCACCTTCTTCGGCAGCTAGCCGCAAAGCACAATGTGACATCGATACTTGTGTTTGGAGATTCTAGTGTCGATCCAGGGAACAATAATTTCATTAAAACCGAAATGAAAGGGAATTTTCCACCTTATGGTGAAAATTTCATCAACCATAAACCCACCGGTAGATTATGCGATGGATTACTCGCTCCCGATTATATTGCGGAGGCCATGGGTTACCCTCCCATACCGGCTTTTCTTGATCCATCCCTAACCCAAGCTGATCTAACTCGCGGTGCAAGTTTTGCCTCTGCTGGTTCTGGCTATGACGATCTCACAGCTAATATATCAAACGTATGGAGTTTCACTACACAAGCCAATTACTTTCTACATTACAAGATTCATCTGACTAAATTGGTCGGTCCATTAGAGAGCGCTAAAATGATAAATAATGCTATATTTCTTATGAGTATGGGATCAAATGACTTTCTTCAAAATTACTTAGTCGATTTTACTCGACAAAAGCAATTCACGGTTGAGCAATACATCGAGTTTCTCTCCCACCGTATGCTTTACGACGCCAAGATGTTGCATAGGCTTGGAGCTAAAAGGTTGGTGGTAGTGGGAGTTCCTCCCATGGGATGCATGCCTTTAATTAAATACCTACGAGGCCAAAAAACTTGTGTAGATCAACTAAACCAAATCGCTTTCTCCTTTAACGCCAAAATCATCAAAAATCTAGAGCTTCTTCAGTCTAAAATCGGTTTGAAAACCATCTACGTTGATGCTTATTCGACCATCCAAGAAGCCATTAAAAATCCGAGAAAATTTGGTTTCGTCGAGGCTTCGCTAGGCTGTTGTGGAACAGGTACATACGAATATGGAGAGACATGCAAAGATATGCAAGTATGCAAAGATCCTACCAAATACGTTTTTTGGGACGCTGTCCATCCAACACAAAGAATGTATCAAATCATTGTTAAGAAGGCAATCGCATCCATCAGTGAAGAGTTTCTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

39.85

Weight (kDa)

8.34

Isoelectric Point (pI)

28.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 345 1.8e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45950
fragaria_vesca FvH4_7g02060
malus_domestica MD10G1286100.v1.1
prunus_persica Prupe.4G055800_v2.0.a1
pyrus_communis pycom10g23880
rosa_laevigata RLG00000031700
rosa_roxburghii Rroxscaffold_1G00066400 Rroxscaffold_6G00404910
rosa_rugosa Rorug04G0445700 Rorug04G0445800
rosa_samantha Rh5AG077300 Rh5BG072400 Rh5CG084800 Rh5DG072900
rosa_wichuraiana Rw5G007120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 203
AccII CGCG 1 cut(s) 345
AciI CCGC 3 cut(s) 99, 275, 345
AclWI GGATC 4 cut(s) 143, 308, 535, 962
AcsI RAATTY 3 cut(s) 190, 211, 878
AcuI CTGAAG 1 cut(s) 785
AcyI GRCGYC 1 cut(s) 630
AdeI CACNNNGTG 1 cut(s) 112
AfaI GTAC 1 cut(s) 922
AfeI AGCGCT 1 cut(s) 487
AfiI CCNNNNNNNGG 2 cut(s) 203, 689
AgeI ACCGGT 1 cut(s) 233
AgsI TTSAA 2 cut(s) 545, 820
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 6 cut(s) 41, 94, 332, 392, 656, 796
AluI AGCT 6 cut(s) 41, 94, 332, 392, 656, 796
Alw26I GTCTC 1 cut(s) 932
AlwI GGATC 4 cut(s) 143, 308, 535, 962
Aor51HI AGCGCT 1 cut(s) 487
AoxI GGCC 2 cut(s) 279, 721
ApeKI GCWGC 2 cut(s) 46, 91
ApoI RAATTY 3 cut(s) 190, 211, 878
AsiGI ACCGGT 1 cut(s) 233
Asp700I GAANNNNTTC 1 cut(s) 1062
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 1 cut(s) 473
AsuHPI GGTGA 2 cut(s) 71, 218
AsuNHI GCTAGC 1 cut(s) 94
AvaII GGWCC 1 cut(s) 473
BaeI ACNNNNGTAYC 2 cut(s) 113, 146
BbvI GCAGC 2 cut(s) 58, 103
BccI CCATC 6 cut(s) 242, 325, 833, 857, 1008, 1058
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 1 cut(s) 153
BcoDI GTCTC 1 cut(s) 932
BfaI CTAG 4 cut(s) 95, 141, 791, 902
BfoI RGCGCY 1 cut(s) 489
BisI GCNGC 3 cut(s) 47, 92, 99
BlsI GCNGC 3 cut(s) 48, 93, 100
Bme1390I CCNGG 1 cut(s) 153
Bme18I GGWCC 1 cut(s) 473
BmgT120I GGNCC 1 cut(s) 473
BmrFI CCNGG 1 cut(s) 153
BmsI GCATC 3 cut(s) 683, 826, 1055
BmtI GCTAGC 1 cut(s) 98
BplI GAGNNNNNCTC 2 cut(s) 593, 625
Bsa29I ATCGAT 1 cut(s) 119
BsaHI GRCGYC 1 cut(s) 630
BsaJI CCNNGG 3 cut(s) 152, 282, 687
BsaWI WCCGGW 1 cut(s) 233
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 689
Bse118I RCCGGY 2 cut(s) 233, 301
BseBI CCWGG 1 cut(s) 153
BseCI ATCGAT 1 cut(s) 119
BseDI CCNNGG 3 cut(s) 152, 282, 687
BseGI GGATG 5 cut(s) 317, 698, 849, 1000, 1046
BseLI CCNNNNNNNGG 2 cut(s) 203, 689
BseXI GCAGC 2 cut(s) 58, 103
Bsh1236I CGCG 1 cut(s) 345
BshFI GGCC 2 cut(s) 281, 723
BshTI ACCGGT 1 cut(s) 233
BshVI ATCGAT 1 cut(s) 119
BsiSI CCGG 2 cut(s) 234, 302
BslFI GGGAC 1 cut(s) 1004
BslI CCNNNNNNNGG 2 cut(s) 203, 689
BsmAI GTCTC 1 cut(s) 932
BsmFI GGGAC 1 cut(s) 1004
BsnI GGCC 2 cut(s) 281, 723
Bsp143I GATC 7 cut(s) 148, 313, 334, 382, 527, 739, 967
Bsp19I CCATGG 2 cut(s) 282, 687
BspACI CCGC 3 cut(s) 99, 275, 345
BspANI GGCC 2 cut(s) 281, 723
BspDI ATCGAT 1 cut(s) 119
BspFNI CGCG 1 cut(s) 345
BspOI GCTAGC 1 cut(s) 98
BspPI GGATC 4 cut(s) 143, 308, 535, 962
BsrFI RCCGGY 2 cut(s) 233, 301
BssAI RCCGGY 2 cut(s) 233, 301
BssECI CCNNGG 3 cut(s) 152, 282, 687
BssMI GATC 7 cut(s) 148, 313, 334, 382, 527, 739, 967
BssNI GRCGYC 1 cut(s) 630
BssT1I CCWWGG 2 cut(s) 282, 687
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 3 cut(s) 57, 586, 617
Bst6I CTCTTC 1 cut(s) 1053
BstACI GRCGYC 1 cut(s) 630
BstAPI GCANNNNNTGC 1 cut(s) 356
BstC8I GCNNGC 2 cut(s) 96, 698
BstDEI CTNAG 1 cut(s) 553
BstDSI CCRYGG 2 cut(s) 282, 687
BstEII GGTNACC 1 cut(s) 287
BstF5I GGATG 5 cut(s) 317, 698, 849, 1000, 1046
BstFNI CGCG 1 cut(s) 345
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 7 cut(s) 151, 316, 337, 385, 530, 742, 970
BstMAI GTCTC 1 cut(s) 932
BstMBI GATC 7 cut(s) 148, 313, 334, 382, 527, 739, 967
BstMWI GCNNNNNNNGC 1 cut(s) 356
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 2 cut(s) 700, 945
BstPI GGTNACC 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 151
BstUI CGCG 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 58, 103
BstX2I RGATCY 1 cut(s) 967
BstYI RGATCY 1 cut(s) 967
Bsu15I ATCGAT 1 cut(s) 119
BsuRI GGCC 2 cut(s) 281, 723
BsuTUI ATCGAT 1 cut(s) 119
BtgI CCRYGG 2 cut(s) 282, 687
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 5 cut(s) 317, 698, 849, 1000, 1046
BtsIMutI CAGTG 2 cut(s) 66, 1060
Cac8I GCNNGC 2 cut(s) 96, 698
CfoI GCGC 1 cut(s) 488
Cfr10I RCCGGY 2 cut(s) 233, 301
Cfr13I GGNCC 1 cut(s) 473
ClaI ATCGAT 1 cut(s) 119
CseI GACGC 2 cut(s) 638, 1001
Csp6I GTAC 1 cut(s) 921
CspAI ACCGGT 1 cut(s) 233
CviAII CATG 4 cut(s) 283, 688, 697, 942
CviQI GTAC 1 cut(s) 921
DdeI CTNAG 1 cut(s) 553
DpnI GATC 7 cut(s) 150, 315, 336, 384, 529, 741, 969
DpnII GATC 7 cut(s) 148, 313, 334, 382, 527, 739, 967
DraIII CACNNNGTG 1 cut(s) 112
Eam1104I CTCTTC 1 cut(s) 1053
EarI CTCTTC 1 cut(s) 1053
Eco130I CCWWGG 2 cut(s) 282, 687
Eco47I GGWCC 1 cut(s) 473
Eco47III AGCGCT 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 785
Eco91I GGTNACC 1 cut(s) 287
EcoO65I GGTNACC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 151
EcoT14I CCWWGG 2 cut(s) 282, 687
EcoT22I ATGCAT 1 cut(s) 698
ErhI CCWWGG 2 cut(s) 282, 687
FaeI CATG 4 cut(s) 286, 691, 700, 945
FalI AAGNNNNNCTT 1 cut(s) 1050
FaqI GGGAC 1 cut(s) 1004
FatI CATG 4 cut(s) 282, 687, 696, 941
Fnu4HI GCNGC 3 cut(s) 47, 92, 99
FokI GGATG 5 cut(s) 304, 705, 836, 987, 1033
Fsp4HI GCNGC 3 cut(s) 47, 92, 99
FspBI CTAG 4 cut(s) 95, 141, 791, 902
GlaI GCGC 1 cut(s) 487
GluI GCNGC 3 cut(s) 47, 92, 99
HaeII RGCGCY 1 cut(s) 489
HaeIII GGCC 2 cut(s) 281, 723
HapII CCGG 2 cut(s) 234, 302
HgaI GACGC 2 cut(s) 638, 1001
HhaI GCGC 1 cut(s) 488
Hin1I GRCGYC 1 cut(s) 630
Hin1II CATG 4 cut(s) 286, 691, 700, 945
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HinfI GANTC 3 cut(s) 22, 137, 451
HpaII CCGG 2 cut(s) 234, 302
HphI GGTGA 2 cut(s) 71, 218
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 2 cut(s) 459, 873
Hpy188III TCNNGA 3 cut(s) 263, 311, 791
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 2 cut(s) 632, 893
HpyAV CCTTC 2 cut(s) 92, 1030
HpyCH4III ACNGT 3 cut(s) 57, 586, 617
HpyCH4IV ACGT 4 cut(s) 75, 405, 831, 981
HpyCH4V TGCA 7 cut(s) 46, 350, 643, 696, 945, 955, 963
HpyF10VI GCNNNNNNNGC 1 cut(s) 356
HpyF3I CTNAG 1 cut(s) 553
HpySE526I ACGT 4 cut(s) 75, 405, 831, 981
Hsp92I GRCGYC 1 cut(s) 630
Hsp92II CATG 4 cut(s) 286, 691, 700, 945
HspAI GCGC 1 cut(s) 486
Kzo9I GATC 7 cut(s) 148, 313, 334, 382, 527, 739, 967
LmnI GCTCC 2 cut(s) 265, 653
LpnPI CCDG 7 cut(s) 138, 165, 247, 315, 351, 357, 903
Lsp1109I GCAGC 2 cut(s) 58, 103
LweI GCATC 3 cut(s) 683, 826, 1055
MaeI CTAG 4 cut(s) 95, 141, 791, 902
MaeII ACGT 4 cut(s) 75, 405, 831, 981
MaeIII GTNAC 3 cut(s) 19, 112, 287
MalI GATC 7 cut(s) 150, 315, 336, 384, 529, 741, 969
MboI GATC 7 cut(s) 148, 313, 334, 382, 527, 739, 967
MboII GAAGA 4 cut(s) 77, 533, 791, 1070
MflI RGATCY 1 cut(s) 967
MluCI AATT 9 cut(s) 163, 190, 211, 430, 464, 547, 578, 705, 878
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 1028
MnlI CCTC 6 cut(s) 271, 303, 370, 693, 713, 886
Mph1103I ATGCAT 1 cut(s) 698
MroXI GAANNNNTTC 1 cut(s) 1062
MseI TTAA 6 cut(s) 171, 704, 708, 768, 864, 1032
MspI CCGG 2 cut(s) 234, 302
MspR9I CCNGG 1 cut(s) 153
MvaI CCWGG 1 cut(s) 153
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 1 cut(s) 356
NcoI CCATGG 2 cut(s) 282, 687
NdeII GATC 7 cut(s) 148, 313, 334, 382, 527, 739, 967
NheI GCTAGC 1 cut(s) 94
NlaIII CATG 4 cut(s) 286, 691, 700, 945
NmuCI GTSAC 2 cut(s) 19, 112
NsiI ATGCAT 1 cut(s) 698
NspI RCATGY 2 cut(s) 700, 945
PacI TTAATTAA 1 cut(s) 708
PaeI GCATGC 1 cut(s) 700
PdmI GAANNNNTTC 1 cut(s) 1062
PfeI GAWTC 2 cut(s) 137, 451
PflFI GACNNNGTC 1 cut(s) 995
PflMI CCANNNNNTGG 1 cut(s) 203
PinAI ACCGGT 1 cut(s) 233
PkrI GCNGC 3 cut(s) 48, 93, 100
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Psp6I CCWGG 1 cut(s) 151
PspEI GGTNACC 1 cut(s) 287
PspGI CCWGG 1 cut(s) 151
PspPI GGNCC 1 cut(s) 473
PsrI GAACNNNNNNTAC 2 cut(s) 663, 695
PsuI RGATCY 1 cut(s) 967
PsyI GACNNNGTC 1 cut(s) 995
RsaI GTAC 1 cut(s) 922
RsaNI GTAC 1 cut(s) 921
SaqAI TTAA 6 cut(s) 171, 704, 708, 768, 864, 1032
SatI GCNGC 3 cut(s) 47, 92, 99
Sau3AI GATC 7 cut(s) 148, 313, 334, 382, 527, 739, 967
Sau96I GGNCC 1 cut(s) 473
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 153
SfaNI GCATC 3 cut(s) 683, 826, 1055
SinI GGWCC 1 cut(s) 473
SphI GCATGC 1 cut(s) 700
Sse9I AATT 9 cut(s) 163, 190, 211, 430, 464, 547, 578, 705, 878
SsiI CCGC 3 cut(s) 99, 275, 345
SspMI CTAG 4 cut(s) 95, 141, 791, 902
StyD4I CCNGG 1 cut(s) 151
StyI CCWWGG 2 cut(s) 282, 687
TaaI ACNGT 3 cut(s) 57, 586, 617
TaiI ACGT 4 cut(s) 78, 408, 834, 984
TaqI TCGA 7 cut(s) 119, 147, 558, 568, 600, 845, 891
TaqII GACCGA 1 cut(s) 461
TasI AATT 9 cut(s) 163, 190, 211, 430, 464, 547, 578, 705, 878
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 2 cut(s) 137, 451
Tru1I TTAA 6 cut(s) 171, 704, 708, 768, 864, 1032
Tru9I TTAA 6 cut(s) 171, 704, 708, 768, 864, 1032
TscAI CASTG 2 cut(s) 66, 1060
TseFI GTSAC 2 cut(s) 19, 112
TseI GCWGC 2 cut(s) 46, 91
Tsp45I GTSAC 2 cut(s) 19, 112
TspDTI ATGAA 4 cut(s) 157, 197, 205, 443
TspRI CASTG 2 cut(s) 66, 1060
Tth111I GACNNNGTC 1 cut(s) 995
Van91I CCANNNNNTGG 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 473
XapI RAATTY 3 cut(s) 190, 211, 878
XbaI TCTAGA 1 cut(s) 790
XceI RCATGY 2 cut(s) 700, 945
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 4 cut(s) 95, 141, 791, 902
Zsp2I ATGCAT 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.