Rorug04G0445700

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0016036
Physical Location & Seq
Forward (+)
60172012 .. 60175405
3394 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0445700.1

Sequence Viewer

Length: 1764 bp
ATGGGTTCTCATGAGGACGGGAGATCGCTATTGGAAGATGGCCTTCTTCAGGTTCGTAAGGATGAAGGCGAGGGATTGTACACAGGAGACGGCTCAGTCGACATTAAGGGAAATCCTGTTCTAAAGCAAAATACTGGAAACTGGAGAGCTTGCCCTTTCATTCTAGGTACCGAATGTTGTGAACGCTTGGCCTACTATGGGATTGCCACTAACCTGGTTACTTATCTTACAAGCAAATTGCATGAAGGAAATGTTGCTGCTGCAAGAAATGTTACCACTTGGCAGGGCACTTGCTATCTTACACCACTCATTGGGGCTGTCTTAGCAGATGCATACTGGGGAAGATACTGGACAATTGCTGTTTTTTCCACCATCTACTTTCTCGGAATGTGTACTCTGACTCTCTCGGCAACACTTCCTTCATTGATTCCACCTGAATGTGTTGGTTCAGCATGCCCTGCAGCTAGTACGTCACAGTATGCAGTGTTCTTCTTTGGGCTCTATCTGATTGCTCTAGGAACTGGTGGAATCAAGCCATGCGTTTCATCCTTTGGGGCGGATCAGTTTGATGATACTGATTCCAGGGAAAGGGTAAAGAAGGGATCTTTCTTCAACTGGTTTTACTTTTCTATCAACATTGGAGCTCTGATATCAAGTAGCTTTATAGTGTATATTCAAGACAATGCTGGATGGGGCCTAGGATTTGGTATTCCTGCATTGTTTATGGCTGTCGCTATTGTTAGTTTCTTTTCAGGCACACCACTCTATAGATTTCAGAGACCAGGGGGAAGCCCTCTTACAAGAATGTGCCAGGTTTTGGTTGCCTCATTTCGTAAGCGGAGTGTGAATGTCCCTGAAGACAGTAGTCTCCTGTATGAAACACAAGATAAAATCTCCGCCATTGAAGGAAGTCGTAAACTGGAGCACAGTGATGAGCTCAAATGTCTTGATAAAGCTGCTGTGATATCAGAAGCAGAGGTCAAAACTGGGGAATTCTCCAATCCATGGAGGCTTTGCACTGTTACACAGGTGGAGGAATTCAAAATTTTAATCCGCATGTTCCCTATCTGGGCTACTGGAATTGTGTTTTCTGCTGTCTATGCCCAAATGTCAACTATGTTTGTGGAACAAGGGACGATGATGGATACATCTATCGGTTCTTTCACCATTCCCCCGGCCTCCCTCTCAACTTTTGATGTCATCAGTGTTATCTTCTGGGTCCCAATTTATGACAGGTTTATTGTCCCAATTGCAAGGAGATTTACTGGTAAAGAAAGGGGGTTCTCAGAGTTGCAAAGGATGGGCATTGGTCTCTTTCTTTCGGTCCTATGCATGTCAGCTGCTGCTGTGGTAGAGATTTACCGATTGCAGATTGCTAAAGAACTTGATTTGGTTGACAAAGAGGTAGCTGTACCACTTAGTATCTTTTGGCAAATTCCTCAATACTTCTTGTTGGGTGCTGCAGAGATATTCACATTCATTGGGCAACTCGAGTTCTTCTACGACCAATCTCCAGATGCCATGCGCAGTTTATGCAGCGCCTTATCACTTCTGACCACTGCACTGGGCAATTATCTGAGCTCTTTCATTCTGACGGTAGTAACTTACTTGACAACACAAGGTGGGAGTTCTGGATGGATCCCTGACAACTTGAATTCAGGTCATCTGGATTATTTCTTCTGGCTCTTAGCAGGACTCAGCTTTATAAACATGGTGGTGTACATGGTCTGCGCCAGGAAATATAAACAGAAGAAGGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

587

Amino Acids

64.92

Weight (kDa)

5.5

Isoelectric Point (pI)

32.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 116 - 547 3.3e-136 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45950
fragaria_vesca FvH4_7g02060
malus_domestica MD10G1286100.v1.1
prunus_persica Prupe.4G055800_v2.0.a1
pyrus_communis pycom10g23880
rosa_laevigata RLG00000031700
rosa_roxburghii Rroxscaffold_1G00066400 Rroxscaffold_6G00404910
rosa_rugosa Rorug04G0445700 Rorug04G0445800
rosa_samantha Rh5AG077300 Rh5BG072400 Rh5CG084800 Rh5DG072900
rosa_wichuraiana Rw5G007120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1706
AasI GACNNNNNNGTC 1 cut(s) 95
Acc16I TGCGCA 1 cut(s) 1526
Acc65I GGTACC 1 cut(s) 167
AccB1I GGYRCC 1 cut(s) 167
AccB7I CCANNNNNTGG 3 cut(s) 311, 817, 1005
AccI GTMKAC 1 cut(s) 99
AciI CCGC 4 cut(s) 557, 838, 897, 1054
AclWI GGATC 4 cut(s) 567, 610, 1633, 1646
AcsI RAATTY 5 cut(s) 992, 1037, 1044, 1434, 1654
AcuI CTGAAG 2 cut(s) 32, 876
AdeI CACNNNGTG 1 cut(s) 1622
AfaI GTAC 6 cut(s) 80, 169, 394, 469, 1413, 1721
AfiI CCNNNNNNNGG 7 cut(s) 49, 198, 311, 588, 817, 1005, 1069
AgsI TTSAA 5 cut(s) 613, 677, 905, 1042, 1654
AjnI CCWGG 5 cut(s) 213, 581, 781, 810, 1733
AloI GAACNNNNNNTCC 2 cut(s) 102, 134
Alw21I GWGCWC 4 cut(s) 646, 927, 939, 1583
Alw26I GTCTC 4 cut(s) 81, 772, 872, 1316
AlwI GGATC 4 cut(s) 567, 610, 1633, 1646
AlwNI CAGNNNCTG 1 cut(s) 1343
Ama87I CYCGRG 1 cut(s) 1490
AoxI GGCC 4 cut(s) 40, 189, 694, 1176
ApeKI GCWGC 8 cut(s) 257, 260, 461, 956, 1340, 1343, 1460, 1536
ApoI RAATTY 5 cut(s) 992, 1037, 1044, 1434, 1654
Asp718I GGTACC 1 cut(s) 167
AspA2I CCTAGG 1 cut(s) 697
AspLEI GCGC 3 cut(s) 1527, 1541, 1733
AspS9I GGNCC 3 cut(s) 694, 1219, 1324
AsuC2I CCSGG 1 cut(s) 1175
AsuHPI GGTGA 1 cut(s) 1156
AvaI CYCGRG 1 cut(s) 1490
AvaII GGWCC 2 cut(s) 1219, 1324
AvrII CCTAGG 1 cut(s) 697
BaeGI GKGCMC 1 cut(s) 290
BamHI GGATCC 1 cut(s) 1638
BanI GGYRCC 1 cut(s) 167
BanII GRGCYC 4 cut(s) 501, 646, 939, 1583
BarI GAAGNNNNNNTAC 2 cut(s) 781, 813
BbsI GAAGAC 1 cut(s) 864
Bbv12I GWGCWC 4 cut(s) 646, 927, 939, 1583
BbvI GCAGC 8 cut(s) 244, 247, 473, 943, 1327, 1330, 1447, 1548
BccI CCATC 6 cut(s) 32, 380, 684, 1135, 1294, 1629
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 5 cut(s) 215, 583, 783, 812, 1735
BciVI GTATCC 1 cut(s) 1138
BcnI CCSGG 1 cut(s) 1175
BcoDI GTCTC 4 cut(s) 81, 772, 872, 1316
BfaI CTAG 4 cut(s) 164, 465, 515, 698
BfmI CTRYAG 3 cut(s) 459, 766, 1461
BfoI RGCGCY 1 cut(s) 1542
BfuI GTATCC 1 cut(s) 1138
BisI GCNGC 8 cut(s) 258, 261, 462, 957, 1341, 1344, 1461, 1537
BlnI CCTAGG 1 cut(s) 697
BlsI GCNGC 8 cut(s) 259, 262, 463, 958, 1342, 1345, 1462, 1538
Bme1390I CCNGG 6 cut(s) 215, 583, 783, 812, 1175, 1735
Bme18I GGWCC 2 cut(s) 1219, 1324
BmeT110I CYCGRG 1 cut(s) 1490
BmgT120I GGNCC 3 cut(s) 694, 1219, 1324
BmiI GGNNCC 5 cut(s) 169, 695, 1220, 1221, 1640
BmrFI CCNGG 6 cut(s) 215, 583, 783, 812, 1175, 1735
BmrI ACTGGG 3 cut(s) 346, 996, 1574
BmsI GCATC 2 cut(s) 319, 1507
BmuI ACTGGG 3 cut(s) 346, 996, 1574
BoxI GACNNNNGTC 1 cut(s) 864
BpiI GAAGAC 1 cut(s) 864
BpmI CTGGAG 3 cut(s) 163, 941, 1497
BpuMI CCSGG 1 cut(s) 1175
BsaI GGTCTC 2 cut(s) 772, 1316
BsaJI CCNNGG 5 cut(s) 582, 697, 782, 1004, 1173
Bsc4I CCNNNNNNNGG 7 cut(s) 49, 198, 311, 588, 817, 1005, 1069
BseBI CCWGG 5 cut(s) 215, 583, 783, 812, 1735
BseDI CCNNGG 5 cut(s) 582, 697, 782, 1004, 1173
BseGI GGATG 5 cut(s) 67, 545, 695, 1305, 1640
BseLI CCNNNNNNNGG 7 cut(s) 49, 198, 311, 588, 817, 1005, 1069
BseMII CTCAG 4 cut(s) 108, 1299, 1568, 1711
BseSI GKGCMC 1 cut(s) 290
BseXI GCAGC 8 cut(s) 244, 247, 473, 943, 1327, 1330, 1447, 1548
BsgI GTGCAG 1 cut(s) 1545
BshFI GGCC 4 cut(s) 42, 191, 696, 1178
BshNI GGYRCC 1 cut(s) 167
BsiHKAI GWGCWC 4 cut(s) 646, 927, 939, 1583
BsiHKCI CYCGRG 1 cut(s) 1490
BsiSI CCGG 1 cut(s) 1175
BslFI GGGAC 4 cut(s) 836, 1147, 1205, 1229
BslI CCNNNNNNNGG 7 cut(s) 49, 198, 311, 588, 817, 1005, 1069
BsmAI GTCTC 4 cut(s) 81, 772, 872, 1316
BsmBI CGTCTC 1 cut(s) 81
BsmFI GGGAC 4 cut(s) 836, 1147, 1205, 1229
BsnI GGCC 4 cut(s) 42, 191, 696, 1178
Bso31I GGTCTC 2 cut(s) 772, 1316
BsoBI CYCGRG 1 cut(s) 1490
Bsp1286I GDGCHC 6 cut(s) 290, 501, 646, 927, 939, 1583
Bsp1407I TGTACA 2 cut(s) 78, 1719
Bsp143I GATC 4 cut(s) 23, 559, 602, 1638
Bsp19I CCATGG 1 cut(s) 1004
BspACI CCGC 4 cut(s) 557, 838, 897, 1054
BspANI GGCC 4 cut(s) 42, 191, 696, 1178
BspCNI CTCAG 4 cut(s) 107, 1298, 1569, 1710
BspHI TCATGA 1 cut(s) 10
BspLI GGNNCC 5 cut(s) 169, 695, 1220, 1221, 1640
BspMAI CTGCAG 2 cut(s) 463, 1465
BspPI GGATC 4 cut(s) 567, 610, 1633, 1646
BspT107I GGYRCC 1 cut(s) 167
BspTNI GGTCTC 2 cut(s) 772, 1316
BsrGI TGTACA 2 cut(s) 78, 1719
BssECI CCNNGG 5 cut(s) 582, 697, 782, 1004, 1173
BssMI GATC 4 cut(s) 23, 559, 602, 1638
BssT1I CCWWGG 2 cut(s) 697, 1004
Bst2UI CCWGG 5 cut(s) 215, 583, 783, 812, 1735
Bst4CI ACNGT 5 cut(s) 477, 863, 929, 1021, 1597
BstAPI GCANNNNNTGC 2 cut(s) 458, 1533
BstAUI TGTACA 2 cut(s) 78, 1719
BstC8I GCNNGC 2 cut(s) 151, 454
BstDEI CTNAG 7 cut(s) 94, 322, 1285, 1418, 1577, 1687, 1697
BstDSI CCRYGG 1 cut(s) 1004
BstENI CCTNNNNNAGG 1 cut(s) 47
BstF5I GGATG 5 cut(s) 67, 545, 695, 1305, 1640
BstH2I RGCGCY 1 cut(s) 1542
BstHHI GCGC 3 cut(s) 1527, 1541, 1733
BstKTI GATC 4 cut(s) 26, 562, 605, 1641
BstMAI GTCTC 4 cut(s) 81, 772, 872, 1316
BstMBI GATC 4 cut(s) 23, 559, 602, 1638
BstMWI GCNNNNNNNGC 4 cut(s) 323, 458, 1100, 1533
BstNI CCWGG 5 cut(s) 215, 583, 783, 812, 1735
BstNSI RCATGY 3 cut(s) 456, 1060, 1336
BstPAI GACNNNNGTC 1 cut(s) 864
BstSCI CCNGG 6 cut(s) 213, 581, 781, 810, 1173, 1733
BstSFI CTRYAG 3 cut(s) 459, 766, 1461
BstSLI GKGCMC 1 cut(s) 290
BstV1I GCAGC 8 cut(s) 244, 247, 473, 943, 1327, 1330, 1447, 1548
BstV2I GAAGAC 1 cut(s) 864
BstX2I RGATCY 2 cut(s) 602, 1638
BstXI CCANNNNNNTGG 2 cut(s) 214, 1564
BstYI RGATCY 2 cut(s) 602, 1638
BsuI GTATCC 1 cut(s) 1138
BsuRI GGCC 4 cut(s) 42, 191, 696, 1178
BtgI CCRYGG 1 cut(s) 1004
BtsCI GGATG 5 cut(s) 67, 545, 695, 1305, 1640
BtsI GCAGTG 2 cut(s) 489, 1557
BtsIMutI CAGTG 6 cut(s) 489, 934, 1017, 1210, 1557, 1562
Cac8I GCNNGC 2 cut(s) 151, 454
CaiI CAGNNNCTG 1 cut(s) 1343
CciI TCATGA 1 cut(s) 10
CfoI GCGC 3 cut(s) 1527, 1541, 1733
Cfr13I GGNCC 3 cut(s) 694, 1219, 1324
CsiI ACCWGGT 1 cut(s) 213
Csp6I GTAC 6 cut(s) 79, 168, 393, 468, 1412, 1720
CviQI GTAC 6 cut(s) 79, 168, 393, 468, 1412, 1720
DdeI CTNAG 7 cut(s) 94, 322, 1285, 1418, 1577, 1687, 1697
DpnI GATC 4 cut(s) 25, 561, 604, 1640
DpnII GATC 4 cut(s) 23, 559, 602, 1638
DraIII CACNNNGTG 1 cut(s) 1622
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
EciI GGCGGA 2 cut(s) 572, 886
Ecl136II GAGCTC 3 cut(s) 644, 937, 1581
Eco130I CCWWGG 2 cut(s) 697, 1004
Eco24I GRGCYC 4 cut(s) 501, 646, 939, 1583
Eco31I GGTCTC 2 cut(s) 772, 1316
Eco32I GATATC 2 cut(s) 651, 966
Eco47I GGWCC 2 cut(s) 1219, 1324
Eco53kI GAGCTC 3 cut(s) 644, 937, 1581
Eco57I CTGAAG 2 cut(s) 32, 876
Eco88I CYCGRG 1 cut(s) 1490
EcoICRI GAGCTC 3 cut(s) 644, 937, 1581
EcoNI CCTNNNNNAGG 1 cut(s) 47
EcoO109I RGGNCCY 2 cut(s) 694, 1219
EcoRI GAATTC 3 cut(s) 992, 1037, 1654
EcoRII CCWGG 5 cut(s) 213, 581, 781, 810, 1733
EcoRV GATATC 2 cut(s) 651, 966
EcoT14I CCWWGG 2 cut(s) 697, 1004
EcoT22I ATGCAT 2 cut(s) 334, 1334
EcoT38I GRGCYC 4 cut(s) 501, 646, 939, 1583
ErhI CCWWGG 2 cut(s) 697, 1004
Esp3I CGTCTC 1 cut(s) 81
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 4 cut(s) 836, 1147, 1205, 1229
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 8 cut(s) 258, 261, 462, 957, 1341, 1344, 1461, 1537
FokI GGATG 5 cut(s) 74, 532, 702, 1312, 1647
FriOI GRGCYC 4 cut(s) 501, 646, 939, 1583
Fsp4HI GCNGC 8 cut(s) 258, 261, 462, 957, 1341, 1344, 1461, 1537
FspBI CTAG 4 cut(s) 164, 465, 515, 698
FspI TGCGCA 1 cut(s) 1526
GlaI GCGC 3 cut(s) 1526, 1540, 1732
GluI GCNGC 8 cut(s) 258, 261, 462, 957, 1341, 1344, 1461, 1537
GsuI CTGGAG 3 cut(s) 163, 941, 1497
HaeII RGCGCY 1 cut(s) 1542
HaeIII GGCC 4 cut(s) 42, 191, 696, 1178
HapII CCGG 1 cut(s) 1175
HhaI GCGC 3 cut(s) 1527, 1541, 1733
Hin6I GCGC 3 cut(s) 1525, 1539, 1731
HinP1I GCGC 3 cut(s) 1525, 1539, 1731
HincII GTYRAC 3 cut(s) 100, 1113, 1396
HindII GTYRAC 3 cut(s) 100, 1113, 1396
HinfI GANTC 5 cut(s) 400, 427, 528, 578, 1695
HpaII CCGG 1 cut(s) 1175
HphI GGTGA 1 cut(s) 1156
Hpy166II GTNNAC 8 cut(s) 81, 100, 182, 393, 917, 1113, 1396, 1720
Hpy188III TCNNGA 6 cut(s) 11, 677, 947, 1514, 1632, 1667
Hpy8I GTNNAC 8 cut(s) 81, 100, 182, 393, 917, 1113, 1396, 1720
HpyAV CCTTC 7 cut(s) 53, 59, 239, 429, 592, 899, 1747
HpyCH4III ACNGT 5 cut(s) 477, 863, 929, 1021, 1597
HpyCH4IV ACGT 1 cut(s) 470
HpyF10VI GCNNNNNNNGC 4 cut(s) 323, 458, 1100, 1533
HpyF3I CTNAG 7 cut(s) 94, 322, 1285, 1418, 1577, 1687, 1697
HpySE526I ACGT 1 cut(s) 470
HspAI GCGC 3 cut(s) 1525, 1539, 1731
KflI GGGWCCC 1 cut(s) 1219
KpnI GGTACC 1 cut(s) 171
Kzo9I GATC 4 cut(s) 23, 559, 602, 1638
LmnI GCTCC 2 cut(s) 641, 922
Lsp1109I GCAGC 8 cut(s) 244, 247, 473, 943, 1327, 1330, 1447, 1548
LweI GCATC 2 cut(s) 319, 1507
MabI ACCWGGT 1 cut(s) 213
MaeI CTAG 4 cut(s) 164, 465, 515, 698
MaeII ACGT 1 cut(s) 470
MaeIII GTNAC 5 cut(s) 217, 271, 471, 1021, 1600
MalI GATC 4 cut(s) 25, 561, 604, 1640
MboI GATC 4 cut(s) 23, 559, 602, 1638
MfeI CAATTG 2 cut(s) 354, 1248
MflI RGATCY 2 cut(s) 602, 1638
MhlI GDGCHC 6 cut(s) 290, 501, 646, 927, 939, 1583
MlyI GAGTC 2 cut(s) 394, 1689
Mph1103I ATGCAT 2 cut(s) 334, 1334
MseI TTAA 3 cut(s) 105, 1049, 1762
MslI CAYNNNNRTG 3 cut(s) 436, 930, 1715
MspA1I CMGCKG 1 cut(s) 1340
MspI CCGG 1 cut(s) 1175
MspR9I CCNGG 6 cut(s) 215, 583, 783, 812, 1175, 1735
MunI CAATTG 2 cut(s) 354, 1248
MvaI CCWGG 5 cut(s) 215, 583, 783, 812, 1735
MwoI GCNNNNNNNGC 4 cut(s) 323, 458, 1100, 1533
NciI CCSGG 1 cut(s) 1175
NcoI CCATGG 1 cut(s) 1004
NdeII GATC 4 cut(s) 23, 559, 602, 1638
NlaIV GGNNCC 5 cut(s) 169, 695, 1220, 1221, 1640
NmeAIII GCCGAG 1 cut(s) 386
NmuCI GTSAC 1 cut(s) 471
NsbI TGCGCA 1 cut(s) 1526
NsiI ATGCAT 2 cut(s) 334, 1334
NspI RCATGY 3 cut(s) 456, 1060, 1336
PaeI GCATGC 1 cut(s) 456
PaeR7I CTCGAG 1 cut(s) 1490
PagI TCATGA 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfeI GAWTC 3 cut(s) 427, 528, 578
PflMI CCANNNNNTGG 3 cut(s) 311, 817, 1005
PkrI GCNGC 8 cut(s) 259, 262, 463, 958, 1342, 1345, 1462, 1538
PleI GAGTC 2 cut(s) 394, 1689
PpsI GAGTC 2 cut(s) 394, 1689
PpuMI RGGWCCY 1 cut(s) 1219
PshAI GACNNNNGTC 1 cut(s) 864
PsiI TTATAA 1 cut(s) 1706
Psp124BI GAGCTC 3 cut(s) 646, 939, 1583
Psp5II RGGWCCY 1 cut(s) 1219
Psp6I CCWGG 5 cut(s) 213, 581, 781, 810, 1733
PspGI CCWGG 5 cut(s) 213, 581, 781, 810, 1733
PspN4I GGNNCC 5 cut(s) 169, 695, 1220, 1221, 1640
PspPI GGNCC 3 cut(s) 694, 1219, 1324
PspPPI RGGWCCY 1 cut(s) 1219
PspXI VCTCGAGB 1 cut(s) 1490
PsrI GAACNNNNNNTAC 2 cut(s) 470, 502
PstI CTGCAG 2 cut(s) 463, 1465
PstNI CAGNNNCTG 1 cut(s) 1343
PsuI RGATCY 2 cut(s) 602, 1638
PvuII CAGCTG 1 cut(s) 1340
RsaI GTAC 6 cut(s) 80, 169, 394, 469, 1413, 1721
RsaNI GTAC 6 cut(s) 79, 168, 393, 468, 1412, 1720
RseI CAYNNNNRTG 3 cut(s) 436, 930, 1715
SacI GAGCTC 3 cut(s) 646, 939, 1583
SalI GTCGAC 1 cut(s) 98
SaqAI TTAA 3 cut(s) 105, 1049, 1762
SatI GCNGC 8 cut(s) 258, 261, 462, 957, 1341, 1344, 1461, 1537
Sau3AI GATC 4 cut(s) 23, 559, 602, 1638
Sau96I GGNCC 3 cut(s) 694, 1219, 1324
SchI GAGTC 2 cut(s) 394, 1689
ScrFI CCNGG 6 cut(s) 215, 583, 783, 812, 1175, 1735
SduI GDGCHC 6 cut(s) 290, 501, 646, 927, 939, 1583
SexAI ACCWGGT 1 cut(s) 213
SfaNI GCATC 2 cut(s) 319, 1507
SfcI CTRYAG 3 cut(s) 459, 766, 1461
Sfr274I CTCGAG 1 cut(s) 1490
SinI GGWCC 2 cut(s) 1219, 1324
SlaI CTCGAG 1 cut(s) 1490
SmiMI CAYNNNNRTG 3 cut(s) 436, 930, 1715
SmlI CTYRAG 1 cut(s) 1490
SmoI CTYRAG 1 cut(s) 1490
SphI GCATGC 1 cut(s) 456
SsiI CCGC 4 cut(s) 557, 838, 897, 1054
SspMI CTAG 4 cut(s) 164, 465, 515, 698
SstI GAGCTC 3 cut(s) 646, 939, 1583
StyD4I CCNGG 6 cut(s) 213, 581, 781, 810, 1173, 1733
StyI CCWWGG 2 cut(s) 697, 1004
TaaI ACNGT 5 cut(s) 477, 863, 929, 1021, 1597
TaiI ACGT 1 cut(s) 473
TaqI TCGA 2 cut(s) 99, 1491
TaqII GACCGA 1 cut(s) 1312
TatI WGTACW 3 cut(s) 78, 392, 1719
TfiI GAWTC 3 cut(s) 427, 528, 578
Tru1I TTAA 3 cut(s) 105, 1049, 1762
Tru9I TTAA 3 cut(s) 105, 1049, 1762
TscAI CASTG 6 cut(s) 489, 934, 1024, 1210, 1564, 1569
TseFI GTSAC 1 cut(s) 471
TseI GCWGC 8 cut(s) 257, 260, 461, 956, 1340, 1343, 1460, 1536
Tsp45I GTSAC 1 cut(s) 471
TspDTI ATGAA 8 cut(s) 78, 148, 258, 411, 534, 891, 1468, 1576
TspRI CASTG 6 cut(s) 489, 934, 1024, 1210, 1564, 1569
Van91I CCANNNNNTGG 3 cut(s) 311, 817, 1005
VpaK11BI GGWCC 2 cut(s) 1219, 1324
XagI CCTNNNNNAGG 1 cut(s) 47
XapI RAATTY 5 cut(s) 992, 1037, 1044, 1434, 1654
XceI RCATGY 3 cut(s) 456, 1060, 1336
XhoI CTCGAG 1 cut(s) 1490
XmaJI CCTAGG 1 cut(s) 697
XmiI GTMKAC 1 cut(s) 99
XspI CTAG 4 cut(s) 164, 465, 515, 698
Zsp2I ATGCAT 2 cut(s) 334, 1334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.