FvH4_7g02060

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016036
Physical Location & Seq
Reverse (-)
2420226 .. 2421983
1758 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02060.t1

Sequence Viewer

Length: 1104 bp
ATGGCAGATGTGAAGATGACGGCGATGATTAGTATGAAAATACTTGCTTTGTTAGCTTTGCTCTCTGGAGCTTTGGGTTCTGCTGAAATGAGGAAAGACGCTGCTCGAATCAATGTGACATGTATCTTAATATTCGGAGACTCCAGTGTCGATCCTGGCAATAACAATGTTCTGCACACCACCATGAAAGGGAATTTTTTTCCTTATGGCAAAGACTTCTTCAATGGCCGCCCAACTGGAAGGTTCAGTAATGGCAGACTTGCCACAGATTTCATTGAATATATATATATCAACTCTTTTGATATAAAGTTGACGGAAGCAGTTGGGTGTACAAAATCAATTCCAGCATTTCTTGACCCAAATTTAAAAGCTGCAGATCTTCTTCATGGTGTTAGTTTTGCATCAGCTGCTTCGGGTTATGATGACCTCACTGCTAATTTTTCGAATGTACTACCTCTTCCTAGACAGATAAAGAGTTTTATGCATTACAAATTGAACTTGACAAGATCGGTGGGCAAGCAGACTGCTGAAGATACCATCAAAAATGCTGTATTTGTTATGAGTATGGGCACAAATGACTTTATCCAAAACTACTACCTGGAACAACTCGCTCCAAGCAATTCACTGTGGAACATTTTCAAAATTACCTGGTCTCTTGCATGGCTCATTCCATTCAGGCTAGGAGGCACAAGGTTGGCTCTTGTTGGGGTCCCACCATTAGGGTGTATGCCACTTGTCAAAACGCTAATGGGCGAGACCAAATGTGTAGAAAGTTTAAACAAAGTTTCATCCTCTTTTAATTCCAAGATACAAAAGAAATTGGAGAACATAACCAAAACATCTGGAATGAAGATTGCGTTTGTTGATGCTTATAGTATTGTTGAAAGTGCCATGAACAACCCAGAGTTATATGGTCTAAGTGAAACTTCAAAAGGGTGTTGTGGGACTGGAACTATAGAATTTGGAGAATCATGCAGAGGGTTAAAAACATGTATTGATCCGTCAAAGTGTGTATTCTGGGATGCTGTTCATCCAACAGAGAAAATGTACAAAATCATTGCAGAGAAAGCTTTGCAAAGTCATGAAGAAATACTCTTTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

368

Amino Acids

40.34

Weight (kDa)

8.46

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 356 3e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45950
fragaria_vesca FvH4_7g02060
malus_domestica MD10G1286100.v1.1
prunus_persica Prupe.4G055800_v2.0.a1
pyrus_communis pycom10g23880
rosa_laevigata RLG00000031700
rosa_roxburghii Rroxscaffold_1G00066400 Rroxscaffold_6G00404910
rosa_rugosa Rorug04G0445700 Rorug04G0445800
rosa_samantha Rh5AG077300 Rh5BG072400 Rh5CG084800 Rh5DG072900
rosa_wichuraiana Rw5G007120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
AciI CCGC 1 cut(s) 229
AclWI GGATC 2 cut(s) 146, 992
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 3 cut(s) 193, 361, 959
AcuI CTGAAG 1 cut(s) 549
AfaI GTAC 3 cut(s) 331, 450, 1049
AfiI CCNNNNNNNGG 2 cut(s) 189, 719
AflIII ACRYGT 2 cut(s) 119, 989
AgsI TTSAA 6 cut(s) 223, 278, 496, 640, 884, 930
AjnI CCWGG 3 cut(s) 154, 597, 647
AluBI AGCT 5 cut(s) 56, 71, 371, 407, 1070
AluI AGCT 5 cut(s) 56, 71, 371, 407, 1070
Alw26I GTCTC 3 cut(s) 132, 657, 749
AlwI GGATC 2 cut(s) 146, 992
AoxI GGCC 1 cut(s) 226
ApeKI GCWGC 3 cut(s) 101, 371, 407
ApoI RAATTY 3 cut(s) 193, 361, 959
Asp700I GAANNNNTTC 1 cut(s) 635
AspS9I GGNCC 1 cut(s) 709
AsuII TTCGAA 1 cut(s) 443
AvaII GGWCC 1 cut(s) 709
BaeGI GKGCMC 1 cut(s) 572
BarI GAAGNNNNNNTAC 2 cut(s) 441, 473
BbvI GCAGC 3 cut(s) 88, 358, 394
BccI CCATC 1 cut(s) 545
BceAI ACGGC 1 cut(s) 36
BciT130I CCWGG 3 cut(s) 156, 599, 649
BcoDI GTCTC 3 cut(s) 132, 657, 749
BfaI CTAG 3 cut(s) 462, 680, 1102
BfmI CTRYAG 2 cut(s) 372, 954
BglII AGATCT 1 cut(s) 376
BisI GCNGC 4 cut(s) 102, 229, 372, 408
BlsI GCNGC 4 cut(s) 103, 230, 373, 409
Bme1390I CCNGG 3 cut(s) 156, 599, 649
Bme18I GGWCC 1 cut(s) 709
BmgT120I GGNCC 1 cut(s) 709
BmiI GGNNCC 2 cut(s) 710, 711
BmrFI CCNGG 3 cut(s) 156, 599, 649
BmsI GCATC 3 cut(s) 410, 856, 1012
BpmI CTGGAG 2 cut(s) 87, 127
Bpu14I TTCGAA 1 cut(s) 443
BsaI GGTCTC 2 cut(s) 657, 749
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 719
Bse1I ACTGG 3 cut(s) 144, 241, 952
Bse3DI GCAATG 1 cut(s) 1056
BseBI CCWGG 3 cut(s) 156, 599, 649
BseGI GGATG 3 cut(s) 788, 1027, 1030
BseLI CCNNNNNNNGG 2 cut(s) 189, 719
BseMI GCAATG 1 cut(s) 1056
BseNI ACTGG 3 cut(s) 144, 241, 952
BseSI GKGCMC 1 cut(s) 572
BseXI GCAGC 3 cut(s) 88, 358, 394
BsgI GTGCAG 1 cut(s) 158
BshFI GGCC 1 cut(s) 228
BslFI GGGAC 2 cut(s) 695, 958
BslI CCNNNNNNNGG 2 cut(s) 189, 719
BsmAI GTCTC 3 cut(s) 132, 657, 749
BsmFI GGGAC 2 cut(s) 695, 958
BsnI GGCC 1 cut(s) 228
Bso31I GGTCTC 2 cut(s) 657, 749
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 1 cut(s) 572
Bsp1407I TGTACA 2 cut(s) 329, 1047
Bsp143I GATC 4 cut(s) 151, 376, 506, 997
BspACI CCGC 1 cut(s) 229
BspANI GGCC 1 cut(s) 228
BspHI TCATGA 1 cut(s) 1081
BspLI GGNNCC 2 cut(s) 710, 711
BspMAI CTGCAG 1 cut(s) 376
BspPI GGATC 2 cut(s) 146, 992
BspT104I TTCGAA 1 cut(s) 443
BspTNI GGTCTC 2 cut(s) 657, 749
BsrDI GCAATG 1 cut(s) 1056
BsrGI TGTACA 2 cut(s) 329, 1047
BsrI ACTGG 3 cut(s) 144, 241, 952
BssMI GATC 4 cut(s) 151, 376, 506, 997
Bst2UI CCWGG 3 cut(s) 156, 599, 649
Bst4CI ACNGT 1 cut(s) 627
Bst6I CTCTTC 1 cut(s) 462
BstAPI GCANNNNNTGC 1 cut(s) 407
BstAUI TGTACA 2 cut(s) 329, 1047
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 1 cut(s) 518
BstDEI CTNAG 1 cut(s) 917
BstF5I GGATG 3 cut(s) 788, 1027, 1030
BstKTI GATC 4 cut(s) 154, 379, 509, 1000
BstMAI GTCTC 3 cut(s) 132, 657, 749
BstMBI GATC 4 cut(s) 151, 376, 506, 997
BstMWI GCNNNNNNNGC 3 cut(s) 53, 407, 1067
BstNI CCWGG 3 cut(s) 156, 599, 649
BstNSI RCATGY 2 cut(s) 123, 993
BstSCI CCNGG 3 cut(s) 154, 597, 647
BstSFI CTRYAG 2 cut(s) 372, 954
BstSLI GKGCMC 1 cut(s) 572
BstV1I GCAGC 3 cut(s) 88, 358, 394
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 1 cut(s) 228
BtgZI GCGATG 1 cut(s) 38
BtsCI GGATG 3 cut(s) 788, 1027, 1030
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 3 cut(s) 151, 429, 623
Cac8I GCNNGC 1 cut(s) 518
CciI TCATGA 1 cut(s) 1081
Cfr13I GGNCC 1 cut(s) 709
CseI GACGC 1 cut(s) 107
CsiI ACCWGGT 1 cut(s) 647
Csp6I GTAC 3 cut(s) 330, 449, 1048
CspCI CAANNNNNGTGG 2 cut(s) 492, 527
CviAII CATG 8 cut(s) 120, 184, 386, 660, 892, 972, 990, 1082
CviJI RGCY 9 cut(s) 56, 71, 228, 371, 407, 664, 679, 698, 1070
CviKI_1 RGCY 9 cut(s) 56, 71, 228, 371, 407, 664, 679, 698, 1070
CviQI GTAC 3 cut(s) 330, 449, 1048
DdeI CTNAG 1 cut(s) 917
DpnI GATC 4 cut(s) 153, 378, 508, 999
DpnII GATC 4 cut(s) 151, 376, 506, 997
DraI TTTAAA 2 cut(s) 366, 777
DrdI GACNNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 1 cut(s) 146
EaeI YGGCCR 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Eco31I GGTCTC 2 cut(s) 657, 749
Eco47I GGWCC 1 cut(s) 709
Eco57I CTGAAG 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 709
EcoRII CCWGG 3 cut(s) 154, 597, 647
EcoT22I ATGCAT 1 cut(s) 486
FaeI CATG 8 cut(s) 123, 187, 389, 663, 895, 975, 993, 1085
FalI AAGNNNNNCTT 2 cut(s) 910, 942
FaqI GGGAC 2 cut(s) 695, 958
FatI CATG 8 cut(s) 119, 183, 385, 659, 891, 971, 989, 1081
Fnu4HI GCNGC 4 cut(s) 102, 229, 372, 408
FokI GGATG 3 cut(s) 775, 1017, 1034
Fsp4HI GCNGC 4 cut(s) 102, 229, 372, 408
FspBI CTAG 3 cut(s) 462, 680, 1102
GluI GCNGC 4 cut(s) 102, 229, 372, 408
GsuI CTGGAG 2 cut(s) 87, 127
HaeIII GGCC 1 cut(s) 228
HgaI GACGC 1 cut(s) 107
Hin1II CATG 8 cut(s) 123, 187, 389, 663, 895, 975, 993, 1085
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HindIII AAGCTT 1 cut(s) 1068
HinfI GANTC 3 cut(s) 108, 140, 968
Hpy166II GTNNAC 2 cut(s) 312, 330
Hpy188I TCNGA 1 cut(s) 137
Hpy188III TCNNGA 4 cut(s) 66, 353, 843, 1082
Hpy8I GTNNAC 2 cut(s) 312, 330
HpyAV CCTTC 1 cut(s) 234
HpyCH4III ACNGT 1 cut(s) 627
HpyCH4V TGCA 8 cut(s) 175, 374, 401, 484, 659, 975, 1061, 1075
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 407, 1067
HpyF3I CTNAG 1 cut(s) 917
Hsp92II CATG 8 cut(s) 123, 187, 389, 663, 895, 975, 993, 1085
KflI GGGWCCC 1 cut(s) 709
Kzo9I GATC 4 cut(s) 151, 376, 506, 997
LmnI GCTCC 2 cut(s) 68, 616
Lsp1109I GCAGC 3 cut(s) 88, 358, 394
LweI GCATC 3 cut(s) 410, 856, 1012
MabI ACCWGGT 1 cut(s) 647
MaeI CTAG 3 cut(s) 462, 680, 1102
MaeIII GTNAC 1 cut(s) 115
MalI GATC 4 cut(s) 153, 378, 508, 999
MboI GATC 4 cut(s) 151, 376, 506, 997
MboII GAAGA 8 cut(s) 25, 211, 371, 374, 449, 542, 862, 1097
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 572
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 1058
MnlI CCTC 6 cut(s) 84, 437, 465, 677, 802, 971
Mph1103I ATGCAT 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 635
MseI TTAA 5 cut(s) 128, 365, 776, 798, 983
MslI CAYNNNNRTG 2 cut(s) 182, 721
MspA1I CMGCKG 1 cut(s) 407
MspR9I CCNGG 3 cut(s) 156, 599, 649
MssI GTTTAAAC 1 cut(s) 777
MvaI CCWGG 3 cut(s) 156, 599, 649
MwoI GCNNNNNNNGC 3 cut(s) 53, 407, 1067
NdeII GATC 4 cut(s) 151, 376, 506, 997
NlaIII CATG 8 cut(s) 123, 187, 389, 663, 895, 975, 993, 1085
NlaIV GGNNCC 2 cut(s) 710, 711
NmuCI GTSAC 1 cut(s) 115
NsiI ATGCAT 1 cut(s) 486
NspI RCATGY 2 cut(s) 123, 993
NspV TTCGAA 1 cut(s) 443
PagI TCATGA 1 cut(s) 1081
PciI ACATGT 2 cut(s) 119, 989
PdmI GAANNNNTTC 1 cut(s) 635
PfeI GAWTC 2 cut(s) 108, 968
PkrI GCNGC 4 cut(s) 103, 230, 373, 409
PleI GAGTC 1 cut(s) 134
PmeI GTTTAAAC 1 cut(s) 777
PpsI GAGTC 1 cut(s) 134
PpuMI RGGWCCY 1 cut(s) 709
PscI ACATGT 2 cut(s) 119, 989
Psp5II RGGWCCY 1 cut(s) 709
Psp6I CCWGG 3 cut(s) 154, 597, 647
PspGI CCWGG 3 cut(s) 154, 597, 647
PspN4I GGNNCC 2 cut(s) 710, 711
PspPI GGNCC 1 cut(s) 709
PspPPI RGGWCCY 1 cut(s) 709
PstI CTGCAG 1 cut(s) 376
PsuI RGATCY 1 cut(s) 376
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 3 cut(s) 331, 450, 1049
RsaNI GTAC 3 cut(s) 330, 449, 1048
RseI CAYNNNNRTG 2 cut(s) 182, 721
SaqAI TTAA 5 cut(s) 128, 365, 776, 798, 983
SatI GCNGC 4 cut(s) 102, 229, 372, 408
Sau3AI GATC 4 cut(s) 151, 376, 506, 997
Sau96I GGNCC 1 cut(s) 709
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 3 cut(s) 156, 599, 649
SduI GDGCHC 1 cut(s) 572
SexAI ACCWGGT 1 cut(s) 647
SfaNI GCATC 3 cut(s) 410, 856, 1012
SfcI CTRYAG 2 cut(s) 372, 954
SfuI TTCGAA 1 cut(s) 443
SinI GGWCC 1 cut(s) 709
SmiMI CAYNNNNRTG 2 cut(s) 182, 721
SsiI CCGC 1 cut(s) 229
SspI AATATT 1 cut(s) 132
SspMI CTAG 3 cut(s) 462, 680, 1102
StyD4I CCNGG 3 cut(s) 154, 597, 647
TaaI ACNGT 1 cut(s) 627
TaqI TCGA 3 cut(s) 106, 150, 443
TatI WGTACW 3 cut(s) 329, 448, 1047
TauI GCSGC 1 cut(s) 231
TfiI GAWTC 2 cut(s) 108, 968
Tru1I TTAA 5 cut(s) 128, 365, 776, 798, 983
Tru9I TTAA 5 cut(s) 128, 365, 776, 798, 983
TscAI CASTG 3 cut(s) 151, 436, 630
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 3 cut(s) 101, 371, 407
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 9 cut(s) 50, 200, 262, 374, 777, 863, 908, 1019, 1098
TspGWI ACGGA 2 cut(s) 329, 990
TspRI CASTG 3 cut(s) 151, 436, 630
VpaK11BI GGWCC 1 cut(s) 709
XapI RAATTY 3 cut(s) 193, 361, 959
XceI RCATGY 2 cut(s) 123, 993
XmnI GAANNNNTTC 1 cut(s) 635
XspI CTAG 3 cut(s) 462, 680, 1102
Zsp2I ATGCAT 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.