MD10G1286100.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016036
Physical Location & Seq
Forward (+)
37554628 .. 37556718
2091 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1286100.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGGTGGCGATGGGTGTGCTGATCGTAACGTTGGGTTTGCTGCTTCCTTTGCTCTCAGGAGCAGTCGACATTGGCCGAGTGAGGGAAGTCGCTGCTCGGAACAACGTGACATGTGTCTTAGTGTTTGGAGATTCAAGTGTTGATCCTGGCAACAACAATGTTCTACCGACCTTCCTGAAGGGCAACTTCCTTCCTTATGGGAAGGACTTCTTCAATGGCCGCCCTACTGGAAGGTTCTCTAATGGAAGACTTGCCACAGATTTTATTGCGGATGCCCTTGGCCACACAAAGATAATCCCAGCATTTCTCGACCCCAATTTGAAGTTGCAAGATCTGCCGCATGGTGTTAGTTTCGCATCAGCTGGTTCCGGCTATGATGATTTGACCGCCAACATTTCGAGTGTATTGTCTCTTCCCAGACAACTGAAGTATTTTTTGCATTACAAAGTCAAGCTGATAAAATTGGTAGGTCAGAAGAAGGCTGAGGATATTATTAGAACTGCTGTACTCATATTGAGTATGGGCACAAATGACTTTGTCCAAAATTACTACTTGGACCCAACTCGACCCAAGCAGTTCAGTGTTGAAGAGTACCAAAATTACTTGGTCTCTTGCATGGCCAATGGCATCCAGCAAATGGAGAGGATAGGAGCGACAAGATTGGTTGTGGTTGGGGTTCCACCATTGGGGTGCATGCCACTAGTGAAAACGCTAATGGACGTGACAGCTTGTGCTGAAAATTACAACAAAGTGGCATTCTCGTTGAGTTCCAGGATACAAAAGAAATTGGAGGCCATAACCAAAACATCCAGAATGAAAATTGGTTTTGTTGATGCTTATGGTATTATGCAAAATGCCATAAATAACCCAAAATTATATGATTTGGCTGAAACTTCAAAAGGATGCTGCGGGACTGGAGCCATGGAAATAGGAGATTCATGCAGGGGACTAGAAACATGCACTGACCCAGCAAAGTACGTATTTTGGGATGCGGTTCATCCAACTGAAAAAATGTACAAAATCATTGCTGACAAGGCTGTCGAAACTCTTTCCGAAACACTCTTTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

38.94

Weight (kDa)

8.5

Isoelectric Point (pI)

25.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 39 - 345 4.7e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45950
fragaria_vesca FvH4_7g02060
malus_domestica MD10G1286100.v1.1
prunus_persica Prupe.4G055800_v2.0.a1
pyrus_communis pycom10g23880
rosa_laevigata RLG00000031700
rosa_roxburghii Rroxscaffold_1G00066400 Rroxscaffold_6G00404910
rosa_rugosa Rorug04G0445700 Rorug04G0445800
rosa_samantha Rh5AG077300 Rh5BG072400 Rh5CG084800 Rh5DG072900
rosa_wichuraiana Rw5G007120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1037
AccB7I CCANNNNNTGG 1 cut(s) 637
AccI GTMKAC 1 cut(s) 66
AciI CCGC 6 cut(s) 220, 269, 338, 387, 909, 992
AclI AACGTT 1 cut(s) 29
AclWI GGATC 1 cut(s) 137
AcoI YGGCCR 4 cut(s) 73, 217, 280, 618
AcuI CTGAAG 2 cut(s) 197, 446
AfaI GTAC 4 cut(s) 507, 593, 977, 1016
AfiI CCNNNNNNNGG 3 cut(s) 82, 637, 686
AflIII ACRYGT 1 cut(s) 110
AgsI TTSAA 5 cut(s) 135, 214, 322, 587, 897
AhlI ACTAGT 1 cut(s) 700
AjiI CACGTC 1 cut(s) 721
AjnI CCWGG 2 cut(s) 145, 770
AluBI AGCT 3 cut(s) 362, 454, 728
AluI AGCT 3 cut(s) 362, 454, 728
Alw26I GTCTC 2 cut(s) 414, 613
AlwI GGATC 1 cut(s) 137
AoxI GGCC 5 cut(s) 73, 217, 280, 618, 792
ApeKI GCWGC 3 cut(s) 40, 92, 906
Asp700I GAANNNNTTC 1 cut(s) 206
AspS9I GGNCC 1 cut(s) 556
AvaII GGWCC 1 cut(s) 556
BaeGI GKGCMC 1 cut(s) 527
BalI TGGCCA 2 cut(s) 282, 620
BarI GAAGNNNNNNTAC 2 cut(s) 396, 428
BbsI GAAGAC 1 cut(s) 253
BbvCI CCTCAGC 1 cut(s) 483
BbvI GCAGC 3 cut(s) 27, 79, 893
BccI CCATC 1 cut(s) 4
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 2 cut(s) 147, 772
BciVI GTATCC 1 cut(s) 768
BcoDI GTCTC 2 cut(s) 414, 613
BcuI ACTAGT 1 cut(s) 700
BfaI CTAG 2 cut(s) 701, 950
BfuI GTATCC 1 cut(s) 768
BglII AGATCT 1 cut(s) 331
BisI GCNGC 5 cut(s) 41, 93, 220, 338, 907
BlsI GCNGC 5 cut(s) 42, 94, 221, 339, 908
Bme1390I CCNGG 2 cut(s) 147, 772
Bme18I GGWCC 1 cut(s) 556
BmgBI CACGTC 1 cut(s) 721
BmgT120I GGNCC 1 cut(s) 556
BmiI GGNNCC 4 cut(s) 367, 558, 678, 919
BmrFI CCNGG 2 cut(s) 147, 772
BmsI GCATC 6 cut(s) 262, 365, 636, 823, 893, 979
BoxI GACNNNNGTC 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 253
BpmI CTGGAG 1 cut(s) 936
Bpu10I CCTNAGC 1 cut(s) 483
BsaAI YACGTR 1 cut(s) 979
BsaI GGTCTC 1 cut(s) 613
BsaJI CCNNGG 2 cut(s) 277, 921
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 637, 686
Bse1I ACTGG 2 cut(s) 232, 919
Bse3DI GCAATG 1 cut(s) 1023
BseBI CCWGG 2 cut(s) 147, 772
BseDI CCNNGG 2 cut(s) 277, 921
BseGI GGATG 6 cut(s) 277, 627, 806, 908, 994, 997
BseLI CCNNNNNNNGG 3 cut(s) 82, 637, 686
BseMI GCAATG 1 cut(s) 1023
BseMII CTCAG 2 cut(s) 69, 474
BseNI ACTGG 2 cut(s) 232, 919
BseSI GKGCMC 1 cut(s) 527
BseXI GCAGC 3 cut(s) 27, 79, 893
BseYI CCCAGC 2 cut(s) 298, 967
BshFI GGCC 5 cut(s) 75, 219, 282, 620, 794
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 2 cut(s) 925, 960
BslI CCNNNNNNNGG 3 cut(s) 82, 637, 686
BsmAI GTCTC 2 cut(s) 414, 613
BsmFI GGGAC 2 cut(s) 925, 960
BsmI GAATGC 1 cut(s) 755
BsnI GGCC 5 cut(s) 75, 219, 282, 620, 794
Bso31I GGTCTC 1 cut(s) 613
Bsp1286I GDGCHC 1 cut(s) 527
Bsp1407I TGTACA 1 cut(s) 1014
Bsp143I GATC 3 cut(s) 21, 142, 331
Bsp19I CCATGG 1 cut(s) 921
BspACI CCGC 6 cut(s) 220, 269, 338, 387, 909, 992
BspANI GGCC 5 cut(s) 75, 219, 282, 620, 794
BspCNI CTCAG 2 cut(s) 68, 475
BspLI GGNNCC 4 cut(s) 367, 558, 678, 919
BspPI GGATC 1 cut(s) 137
BspTNI GGTCTC 1 cut(s) 613
BsrDI GCAATG 1 cut(s) 1023
BsrGI TGTACA 1 cut(s) 1014
BsrI ACTGG 2 cut(s) 232, 919
BssECI CCNNGG 2 cut(s) 277, 921
BssMI GATC 3 cut(s) 21, 142, 331
BssT1I CCWWGG 2 cut(s) 277, 921
Bst2UI CCWGG 2 cut(s) 147, 772
Bst6I CTCTTC 2 cut(s) 417, 582
BstAPI GCANNNNNTGC 1 cut(s) 334
BstAUI TGTACA 1 cut(s) 1014
BstBAI YACGTR 1 cut(s) 979
BstC8I GCNNGC 1 cut(s) 695
BstDEI CTNAG 3 cut(s) 55, 118, 483
BstDSI CCRYGG 1 cut(s) 921
BstF5I GGATG 6 cut(s) 277, 627, 806, 908, 994, 997
BstKTI GATC 3 cut(s) 24, 145, 334
BstMAI GTCTC 2 cut(s) 414, 613
BstMBI GATC 3 cut(s) 21, 142, 331
BstMWI GCNNNNNNNGC 3 cut(s) 49, 334, 1034
BstNI CCWGG 2 cut(s) 147, 772
BstNSI RCATGY 3 cut(s) 114, 697, 960
BstPAI GACNNNNGTC 1 cut(s) 113
BstSCI CCNGG 2 cut(s) 145, 770
BstSLI GKGCMC 1 cut(s) 527
BstSNI TACGTA 1 cut(s) 979
BstV1I GCAGC 3 cut(s) 27, 79, 893
BstV2I GAAGAC 1 cut(s) 253
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BsuI GTATCC 1 cut(s) 768
BsuRI GGCC 5 cut(s) 75, 219, 282, 620, 794
BtgI CCRYGG 1 cut(s) 921
BtgZI GCGATG 1 cut(s) 23
BtrI CACGTC 1 cut(s) 721
BtsCI GGATG 6 cut(s) 277, 627, 806, 908, 994, 997
BtsIMutI CAGTG 2 cut(s) 586, 960
Cac8I GCNNGC 1 cut(s) 695
Cfr13I GGNCC 1 cut(s) 556
Csp6I GTAC 4 cut(s) 506, 592, 976, 1015
CviAII CATG 7 cut(s) 111, 341, 616, 694, 922, 939, 957
CviQI GTAC 4 cut(s) 506, 592, 976, 1015
DdeI CTNAG 3 cut(s) 55, 118, 483
DpnI GATC 3 cut(s) 23, 144, 333
DpnII GATC 3 cut(s) 21, 142, 331
DrdI GACNNNNNNGTC 1 cut(s) 1037
DseDI GACNNNNNNGTC 1 cut(s) 1037
EaeI YGGCCR 4 cut(s) 73, 217, 280, 618
Eam1104I CTCTTC 2 cut(s) 417, 582
EarI CTCTTC 2 cut(s) 417, 582
Eco105I TACGTA 1 cut(s) 979
Eco130I CCWWGG 2 cut(s) 277, 921
Eco31I GGTCTC 1 cut(s) 613
Eco47I GGWCC 1 cut(s) 556
Eco57I CTGAAG 2 cut(s) 197, 446
EcoRII CCWGG 2 cut(s) 145, 770
EcoT14I CCWWGG 2 cut(s) 277, 921
ErhI CCWWGG 2 cut(s) 277, 921
FaeI CATG 7 cut(s) 114, 344, 619, 697, 925, 942, 960
FalI AAGNNNNNCTT 4 cut(s) 170, 202, 194, 226
FaqI GGGAC 2 cut(s) 925, 960
FatI CATG 7 cut(s) 110, 340, 615, 693, 921, 938, 956
FauI CCCGC 1 cut(s) 902
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 5 cut(s) 41, 93, 220, 338, 907
FokI GGATG 6 cut(s) 284, 614, 793, 915, 984, 1001
Fsp4HI GCNGC 5 cut(s) 41, 93, 220, 338, 907
FspBI CTAG 2 cut(s) 701, 950
GluI GCNGC 5 cut(s) 41, 93, 220, 338, 907
GsaI CCCAGC 2 cut(s) 302, 971
GsuI CTGGAG 1 cut(s) 936
HaeIII GGCC 5 cut(s) 75, 219, 282, 620, 794
HapII CCGG 1 cut(s) 369
Hin1II CATG 7 cut(s) 114, 344, 619, 697, 925, 942, 960
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 2 cut(s) 131, 935
HpaII CCGG 1 cut(s) 369
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 3 cut(s) 99, 474, 1054
Hpy188III TCNNGA 4 cut(s) 57, 175, 308, 810
Hpy8I GTNNAC 1 cut(s) 67
HpyAV CCTTC 6 cut(s) 172, 181, 196, 200, 225, 472
HpyCH4IV ACGT 4 cut(s) 29, 105, 720, 978
HpyCH4V TGCA 7 cut(s) 328, 439, 615, 693, 850, 942, 960
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 334, 1034
HpyF3I CTNAG 3 cut(s) 55, 118, 483
HpySE526I ACGT 4 cut(s) 29, 105, 720, 978
Hsp92II CATG 7 cut(s) 114, 344, 619, 697, 925, 942, 960
Kzo9I GATC 3 cut(s) 21, 142, 331
LmnI GCTCC 3 cut(s) 59, 650, 917
Lsp1109I GCAGC 3 cut(s) 27, 79, 893
LweI GCATC 6 cut(s) 262, 365, 636, 823, 893, 979
MaeI CTAG 2 cut(s) 701, 950
MaeII ACGT 4 cut(s) 29, 105, 720, 978
MaeIII GTNAC 3 cut(s) 25, 106, 721
MalI GATC 3 cut(s) 23, 144, 333
MboI GATC 3 cut(s) 21, 142, 331
MboII GAAGA 5 cut(s) 202, 258, 404, 487, 599
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 1 cut(s) 527
MlsI TGGCCA 2 cut(s) 282, 620
MluCI AATT 8 cut(s) 316, 461, 544, 598, 739, 785, 819, 872
MluNI TGGCCA 2 cut(s) 282, 620
MmeI TCCRAC 1 cut(s) 1025
MnlI CCTC 4 cut(s) 75, 478, 636, 784
Mox20I TGGCCA 2 cut(s) 282, 620
MroXI GAANNNNTTC 1 cut(s) 206
MscI TGGCCA 2 cut(s) 282, 620
MslI CAYNNNNRTG 1 cut(s) 688
Msp20I TGGCCA 2 cut(s) 282, 620
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 2 cut(s) 147, 772
Mva1269I GAATGC 1 cut(s) 755
MvaI CCWGG 2 cut(s) 147, 772
MwoI GCNNNNNNNGC 3 cut(s) 49, 334, 1034
NcoI CCATGG 1 cut(s) 921
NdeII GATC 3 cut(s) 21, 142, 331
NlaIII CATG 7 cut(s) 114, 344, 619, 697, 925, 942, 960
NlaIV GGNNCC 4 cut(s) 367, 558, 678, 919
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 2 cut(s) 106, 721
NspI RCATGY 3 cut(s) 114, 697, 960
PaeI GCATGC 1 cut(s) 697
PciI ACATGT 1 cut(s) 110
PctI GAATGC 1 cut(s) 755
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 2 cut(s) 131, 935
PflFI GACNNNGTC 1 cut(s) 536
PflMI CCANNNNNTGG 1 cut(s) 637
PfoI TCCNGGA 1 cut(s) 770
PkrI GCNGC 5 cut(s) 42, 94, 221, 339, 908
Ppu21I YACGTR 1 cut(s) 979
PscI ACATGT 1 cut(s) 110
PshAI GACNNNNGTC 1 cut(s) 113
Psp1406I AACGTT 1 cut(s) 29
Psp6I CCWGG 2 cut(s) 145, 770
PspFI CCCAGC 2 cut(s) 298, 967
PspGI CCWGG 2 cut(s) 145, 770
PspN4I GGNNCC 4 cut(s) 367, 558, 678, 919
PspPI GGNCC 1 cut(s) 556
PsuI RGATCY 1 cut(s) 331
PsyI GACNNNGTC 1 cut(s) 536
PvuII CAGCTG 1 cut(s) 362
RsaI GTAC 4 cut(s) 507, 593, 977, 1016
RsaNI GTAC 4 cut(s) 506, 592, 976, 1015
RseI CAYNNNNRTG 1 cut(s) 688
SalI GTCGAC 1 cut(s) 65
SatI GCNGC 5 cut(s) 41, 93, 220, 338, 907
Sau3AI GATC 3 cut(s) 21, 142, 331
Sau96I GGNCC 1 cut(s) 556
ScrFI CCNGG 2 cut(s) 147, 772
SduI GDGCHC 1 cut(s) 527
SfaNI GCATC 6 cut(s) 262, 365, 636, 823, 893, 979
SinI GGWCC 1 cut(s) 556
SmiMI CAYNNNNRTG 1 cut(s) 688
SnaBI TACGTA 1 cut(s) 979
SpeI ACTAGT 1 cut(s) 700
SphI GCATGC 1 cut(s) 697
Sse9I AATT 8 cut(s) 316, 461, 544, 598, 739, 785, 819, 872
SsiI CCGC 6 cut(s) 220, 269, 338, 387, 909, 992
SspMI CTAG 2 cut(s) 701, 950
StyD4I CCNGG 2 cut(s) 145, 770
StyI CCWWGG 2 cut(s) 277, 921
TaiI ACGT 4 cut(s) 32, 108, 723, 981
TaqI TCGA 5 cut(s) 66, 309, 398, 565, 1041
TasI AATT 8 cut(s) 316, 461, 544, 598, 739, 785, 819, 872
TatI WGTACW 2 cut(s) 505, 1014
TauI GCSGC 2 cut(s) 222, 340
TfiI GAWTC 2 cut(s) 131, 935
TscAI CASTG 2 cut(s) 586, 967
TseFI GTSAC 2 cut(s) 106, 721
TseI GCWGC 3 cut(s) 40, 92, 906
Tsp45I GTSAC 2 cut(s) 106, 721
TspDTI ATGAA 3 cut(s) 830, 927, 986
TspRI CASTG 2 cut(s) 586, 967
Tth111I GACNNNGTC 1 cut(s) 536
Van91I CCANNNNNTGG 1 cut(s) 637
VpaK11BI GGWCC 1 cut(s) 556
XceI RCATGY 3 cut(s) 114, 697, 960
XmiI GTMKAC 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 2 cut(s) 701, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.