Rh5AG077300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0016036
Physical Location & Seq
Reverse (-)
6616278 .. 6618249
1972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG077300.1

Sequence Viewer

Length: 468 bp
ATGGAACTAATCTGTCCAACTTTCTTGCAGGCAATGCATAAGCTAGGAGGCACAAGATTGGCTGTTGTTGGAGTTCCACCATTGGGGTGCATGCCACTTGTCAAAACGCTTATGGGAGAGACCAAATGTGTGGAAAATTATAACAAAGTGTCATTCTCTTTTAATTCCAAGATTCAAAAGAAATTAGAGATCATTACCAAAACATCTGGAATGAAGATTGCCTTTGTTGATGCTTATGGTATTGTCGAAAGTGCCATGAATAACCCACAGTTATATGGTCTAACTGAAACTTCAAAAGGGTGTTGTGGGACTGGAACTATAGAGTTTGGAGAATCATGCAGAGGATTAAAAACATGCACTGATCCAGCAAAGTATGTATTCTGGGATGCTGTTCATCCAACAGAGAAAATGTACGAAATCGTTGCGGGGAAAGCTTTGCAAACTCTTGATGGAAAACTCTCTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

155

Amino Acids

16.85

Weight (kDa)

8.44

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 7 - 142 4.5e-16 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45950
fragaria_vesca FvH4_7g02060
malus_domestica MD10G1286100.v1.1
prunus_persica Prupe.4G055800_v2.0.a1
pyrus_communis pycom10g23880
rosa_laevigata RLG00000031700
rosa_roxburghii Rroxscaffold_1G00066400 Rroxscaffold_6G00404910
rosa_rugosa Rorug04G0445700 Rorug04G0445800
rosa_samantha Rh5AG077300 Rh5BG072400 Rh5CG084800 Rh5DG072900
rosa_wichuraiana Rw5G007120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 141
AciI CCGC 1 cut(s) 425
AclWI GGATC 1 cut(s) 356
AfaI GTAC 1 cut(s) 413
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 2 cut(s) 176, 294
AluBI AGCT 2 cut(s) 43, 434
AluI AGCT 2 cut(s) 43, 434
Alw26I GTCTC 1 cut(s) 113
AlwI GGATC 1 cut(s) 356
BccI CCATC 1 cut(s) 443
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BcoDI GTCTC 1 cut(s) 113
BfaI CTAG 2 cut(s) 44, 466
BfmI CTRYAG 1 cut(s) 318
BmsI GCATC 2 cut(s) 220, 376
BsaI GGTCTC 1 cut(s) 113
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 316
Bse3DI GCAATG 1 cut(s) 39
BseGI GGATG 2 cut(s) 391, 394
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMI GCAATG 1 cut(s) 39
BseNI ACTGG 1 cut(s) 316
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 322
Bso31I GGTCTC 1 cut(s) 113
Bsp143I GATC 2 cut(s) 189, 361
BspACI CCGC 1 cut(s) 425
BspPI GGATC 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 1 cut(s) 316
BssMI GATC 2 cut(s) 189, 361
Bst4CI ACNGT 1 cut(s) 270
BstAPI GCANNNNNTGC 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 30, 92
BstF5I GGATG 2 cut(s) 391, 394
BstKTI GATC 2 cut(s) 192, 364
BstMAI GTCTC 1 cut(s) 113
BstMBI GATC 2 cut(s) 189, 361
BstMWI GCNNNNNNNGC 2 cut(s) 34, 431
BstNSI RCATGY 2 cut(s) 94, 357
BstSFI CTRYAG 1 cut(s) 318
BstXI CCANNNNNNTGG 1 cut(s) 130
BtsCI GGATG 2 cut(s) 391, 394
BtsIMutI CAGTG 1 cut(s) 357
Cac8I GCNNGC 2 cut(s) 30, 92
Csp6I GTAC 1 cut(s) 412
CviAII CATG 4 cut(s) 91, 256, 336, 354
CviJI RGCY 3 cut(s) 43, 62, 434
CviKI_1 RGCY 3 cut(s) 43, 62, 434
CviQI GTAC 1 cut(s) 412
DpnI GATC 2 cut(s) 191, 363
DpnII GATC 2 cut(s) 189, 361
Eco31I GGTCTC 1 cut(s) 113
EcoT22I ATGCAT 1 cut(s) 39
FaeI CATG 4 cut(s) 94, 259, 339, 357
FalI AAGNNNNNCTT 2 cut(s) 206, 238
FaqI GGGAC 1 cut(s) 322
FatI CATG 4 cut(s) 90, 255, 335, 353
FauI CCCGC 1 cut(s) 418
FokI GGATG 2 cut(s) 381, 398
FspBI CTAG 2 cut(s) 44, 466
Hin1II CATG 4 cut(s) 94, 259, 339, 357
HindIII AAGCTT 1 cut(s) 432
HinfI GANTC 2 cut(s) 172, 332
Hpy188III TCNNGA 2 cut(s) 207, 446
HpyCH4III ACNGT 1 cut(s) 270
HpyCH4V TGCA 6 cut(s) 28, 37, 90, 339, 357, 439
HpyF10VI GCNNNNNNNGC 2 cut(s) 34, 431
Hsp92II CATG 4 cut(s) 94, 259, 339, 357
Kzo9I GATC 2 cut(s) 189, 361
LpnPI CCDG 5 cut(s) 14, 192, 297, 367, 378
LweI GCATC 2 cut(s) 220, 376
MaeI CTAG 2 cut(s) 44, 466
MalI GATC 2 cut(s) 191, 363
MboI GATC 2 cut(s) 189, 361
MboII GAAGA 1 cut(s) 226
MluCI AATT 3 cut(s) 136, 163, 182
MmeI TCCRAC 3 cut(s) 41, 49, 422
MnlI CCTC 2 cut(s) 41, 335
Mph1103I ATGCAT 1 cut(s) 39
MseI TTAA 2 cut(s) 162, 347
MslI CAYNNNNRTG 1 cut(s) 85
MwoI GCNNNNNNNGC 2 cut(s) 34, 431
NdeII GATC 2 cut(s) 189, 361
NlaIII CATG 4 cut(s) 94, 259, 339, 357
NsiI ATGCAT 1 cut(s) 39
NspI RCATGY 2 cut(s) 94, 357
PaeI GCATGC 1 cut(s) 94
PfeI GAWTC 2 cut(s) 172, 332
PsiI TTATAA 1 cut(s) 141
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
RseI CAYNNNNRTG 1 cut(s) 85
SaqAI TTAA 2 cut(s) 162, 347
Sau3AI GATC 2 cut(s) 189, 361
SetI ASST 2 cut(s) 45, 436
SfaNI GCATC 2 cut(s) 220, 376
SfcI CTRYAG 1 cut(s) 318
SmiMI CAYNNNNRTG 1 cut(s) 85
SphI GCATGC 1 cut(s) 94
Sse9I AATT 3 cut(s) 136, 163, 182
SsiI CCGC 1 cut(s) 425
SspMI CTAG 2 cut(s) 44, 466
TaaI ACNGT 1 cut(s) 270
TaqI TCGA 1 cut(s) 246
TasI AATT 3 cut(s) 136, 163, 182
TfiI GAWTC 2 cut(s) 172, 332
Tru1I TTAA 2 cut(s) 162, 347
Tru9I TTAA 2 cut(s) 162, 347
TscAI CASTG 1 cut(s) 364
TspDTI ATGAA 3 cut(s) 227, 272, 383
TspRI CASTG 1 cut(s) 364
XceI RCATGY 2 cut(s) 94, 357
XspI CTAG 2 cut(s) 44, 466
Zsp2I ATGCAT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.