Rw5G007120

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016036
Physical Location & Seq
Reverse (-)
7393699 .. 7395417
1719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G007120.1

Sequence Viewer

Length: 1056 bp
ATGGCCGAGATGAGGATGATGATGATTAGTTTGACAATACTAACTTCGCTGCCTTTGCTCTCTGGAGCTTTGGATGCTGCTGAGATGAGGAAAGTTGCTGCTCGAAACAATGTGACATGTATCTTAGCATTCGGAGACTCCAGTGTCGATCCTGGCAATAACAACGTTCTGCACACGAGCATGAAAGGGAATTTCCTTCCTTATGGCAAAGACTTCTTCAATGGGCGCCCAACTGGAAGGTTCAGTAGTAATGGCAGACTTGCCACAGATTTCATTGCGGACGCAGTTGGGTATACAAAAACAATTCCAGCATTTCTTGATCCAAATTTAAAGCCTGAAGATCTGCCTCATGGTGTTAGTTTTGCATCAGCTGCCTCGGGTTATGACGACCTCACTGCCAATTTTTCGCTGAAGAGTTTTATGCATTATAAATTAAACTTGACAAGATTGGTGGGTAAGAAGACTGCTGAAGATACCATCAAAAATGCCTCATTTGTTATGAGTATGGGAACAAATGACTTTATCCAAAACTACTACTTGGAACCAACTCGCTCCAAACAGTTCACTGTGGAACAGTATCAAAATTATTTGGTCTCTTGCATGGCTCATTCCATTCAGGCAATGCATAAGCTAGGAGGCACAAGATTGGCTGTTGTTGGAGTTCCACCATTGGGGTGCATGCCACTTGTCAAAACGCTTATGGGAGAGACCAAATGTGTGGAAAATTATAACAAAGTGTCATTCTCTTTTAATTCCAAGATTCAAAAGAAATTAGAGATCATTACCAAAACATCTGGAATGAAGATTGCCTTTGTTGATGCTTATGGTATTGTCGAAAGTGCCATGAATAACCCACAGTTATATGGTCTAACTGAAACTTCAAAAGGGTGTTGTGGGACTGGAACTATAGAGTTTGGAGAATCATGCAGAGGATTAAAAACATGCACTGATCCAGCAAAGTATGTATTCTGGGATGCTGTTCATCCAACAGAGAAAATGTACGAAATCGTTGCGGGGAAAGCTTTGCAAACTCTTGATGGAAAACTCTCTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

38.4

Weight (kDa)

8.64

Isoelectric Point (pI)

27.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 338 3.9e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45950
fragaria_vesca FvH4_7g02060
malus_domestica MD10G1286100.v1.1
prunus_persica Prupe.4G055800_v2.0.a1
pyrus_communis pycom10g23880
rosa_laevigata RLG00000031700
rosa_roxburghii Rroxscaffold_1G00066400 Rroxscaffold_6G00404910
rosa_rugosa Rorug04G0445700 Rorug04G0445800
rosa_samantha Rh5AG077300 Rh5BG072400 Rh5CG084800 Rh5DG072900
rosa_wichuraiana Rw5G007120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 429, 729
AasI GACNNNNNNGTC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 225
AccI GTMKAC 1 cut(s) 293
AciI CCGC 2 cut(s) 278, 1013
AclI AACGTT 1 cut(s) 165
AclWI GGATC 3 cut(s) 143, 314, 944
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 190, 325
AcuI CTGAAG 3 cut(s) 357, 431, 489
AcyI GRCGYC 1 cut(s) 226
AfaI GTAC 1 cut(s) 1001
AfiI CCNNNNNNNGG 2 cut(s) 12, 671
AflIII ACRYGT 1 cut(s) 116
AgsI TTSAA 3 cut(s) 220, 764, 882
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 4 cut(s) 68, 371, 631, 1022
AluI AGCT 4 cut(s) 68, 371, 631, 1022
Alw26I GTCTC 3 cut(s) 129, 598, 701
AlwI GGATC 3 cut(s) 143, 314, 944
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 4 cut(s) 49, 77, 98, 371
ApoI RAATTY 2 cut(s) 190, 325
AspLEI GCGC 1 cut(s) 228
AvaI CYCGRG 1 cut(s) 376
BanI GGYRCC 1 cut(s) 225
BauI CACGAG 1 cut(s) 175
BbsI GAAGAC 1 cut(s) 467
BbvI GCAGC 4 cut(s) 36, 64, 85, 358
BccI CCATC 2 cut(s) 485, 1031
BcgI CGANNNNNNTGC 4 cut(s) 377, 411, 992, 1026
BciT130I CCWGG 1 cut(s) 153
BcoDI GTCTC 3 cut(s) 129, 598, 701
BfaI CTAG 2 cut(s) 632, 1054
BfmI CTRYAG 1 cut(s) 906
BfoI RGCGCY 1 cut(s) 229
BglII AGATCT 1 cut(s) 340
BisI GCNGC 4 cut(s) 50, 78, 99, 372
BlsI GCNGC 4 cut(s) 51, 79, 100, 373
Bme1390I CCNGG 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 376
BmiI GGNNCC 2 cut(s) 227, 543
BmrFI CCNGG 1 cut(s) 153
BmsI GCATC 4 cut(s) 64, 374, 808, 964
BpiI GAAGAC 1 cut(s) 467
BpmI CTGGAG 2 cut(s) 84, 124
BsaHI GRCGYC 1 cut(s) 226
BsaI GGTCTC 2 cut(s) 598, 701
BsaJI CCNNGG 1 cut(s) 375
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 671
Bse1I ACTGG 3 cut(s) 141, 238, 904
Bse3DI GCAATG 2 cut(s) 273, 627
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 375
BseGI GGATG 4 cut(s) 21, 79, 979, 982
BseLI CCNNNNNNNGG 2 cut(s) 12, 671
BseMI GCAATG 2 cut(s) 273, 627
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 3 cut(s) 141, 238, 904
BseXI GCAGC 4 cut(s) 36, 64, 85, 358
BsgI GTGCAG 1 cut(s) 155
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 376
BslFI GGGAC 1 cut(s) 910
BslI CCNNNNNNNGG 2 cut(s) 12, 671
BsmAI GTCTC 3 cut(s) 129, 598, 701
BsmFI GGGAC 1 cut(s) 910
BsmI GAATGC 1 cut(s) 128
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 2 cut(s) 598, 701
BsoBI CYCGRG 1 cut(s) 376
Bsp143I GATC 5 cut(s) 148, 319, 340, 777, 949
BspACI CCGC 2 cut(s) 278, 1013
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 73
BspLI GGNNCC 2 cut(s) 227, 543
BspPI GGATC 3 cut(s) 143, 314, 944
BspT107I GGYRCC 1 cut(s) 225
BspTNI GGTCTC 2 cut(s) 598, 701
BsrDI GCAATG 2 cut(s) 273, 627
BsrI ACTGG 3 cut(s) 141, 238, 904
BssECI CCNNGG 1 cut(s) 375
BssMI GATC 5 cut(s) 148, 319, 340, 777, 949
BssNAI GTATAC 1 cut(s) 294
BssNI GRCGYC 1 cut(s) 226
BssSI CACGAG 1 cut(s) 175
Bst1107I GTATAC 1 cut(s) 294
Bst2BI CACGAG 1 cut(s) 175
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 4 cut(s) 561, 568, 576, 858
Bst6I CTCTTC 1 cut(s) 407
BstACI GRCGYC 1 cut(s) 226
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 1 cut(s) 680
BstDEI CTNAG 2 cut(s) 81, 124
BstF5I GGATG 4 cut(s) 21, 79, 979, 982
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 5 cut(s) 151, 322, 343, 780, 952
BstMAI GTCTC 3 cut(s) 129, 598, 701
BstMBI GATC 5 cut(s) 148, 319, 340, 777, 949
BstMWI GCNNNNNNNGC 4 cut(s) 55, 74, 371, 1019
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 3 cut(s) 120, 682, 945
BstSCI CCNGG 1 cut(s) 151
BstSFI CTRYAG 1 cut(s) 906
BstV1I GCAGC 4 cut(s) 36, 64, 85, 358
BstV2I GAAGAC 1 cut(s) 467
BstX2I RGATCY 1 cut(s) 340
BstXI CCANNNNNNTGG 1 cut(s) 718
BstYI RGATCY 1 cut(s) 340
BstZ17I GTATAC 1 cut(s) 294
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 4 cut(s) 21, 79, 979, 982
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 4 cut(s) 148, 393, 564, 945
Cac8I GCNNGC 1 cut(s) 680
CfoI GCGC 1 cut(s) 228
CseI GACGC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 1000
CspCI CAANNNNNGTGG 2 cut(s) 432, 467
CviAII CATG 8 cut(s) 117, 181, 350, 601, 679, 844, 924, 942
CviJI RGCY 8 cut(s) 5, 68, 334, 371, 605, 631, 650, 1022
CviKI_1 RGCY 8 cut(s) 5, 68, 334, 371, 605, 631, 650, 1022
CviQI GTAC 1 cut(s) 1000
DdeI CTNAG 2 cut(s) 81, 124
DinI GGCGCC 1 cut(s) 227
DpnI GATC 5 cut(s) 150, 321, 342, 779, 951
DpnII GATC 5 cut(s) 148, 319, 340, 777, 949
DraI TTTAAA 1 cut(s) 330
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 407
EarI CTCTTC 1 cut(s) 407
Eco31I GGTCTC 2 cut(s) 598, 701
Eco57I CTGAAG 3 cut(s) 357, 431, 489
Eco88I CYCGRG 1 cut(s) 376
EcoRII CCWGG 1 cut(s) 151
EcoT22I ATGCAT 2 cut(s) 426, 627
EgeI GGCGCC 1 cut(s) 227
EheI GGCGCC 1 cut(s) 227
FaeI CATG 8 cut(s) 120, 184, 353, 604, 682, 847, 927, 945
FalI AAGNNNNNCTT 2 cut(s) 794, 826
FaqI GGGAC 1 cut(s) 910
FatI CATG 8 cut(s) 116, 180, 349, 600, 678, 843, 923, 941
FauI CCCGC 1 cut(s) 1006
FblI GTMKAC 1 cut(s) 293
Fnu4HI GCNGC 4 cut(s) 50, 78, 99, 372
FokI GGATG 4 cut(s) 28, 86, 969, 986
Fsp4HI GCNGC 4 cut(s) 50, 78, 99, 372
FspBI CTAG 2 cut(s) 632, 1054
GlaI GCGC 1 cut(s) 227
GluI GCNGC 4 cut(s) 50, 78, 99, 372
GsuI CTGGAG 2 cut(s) 84, 124
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 1 cut(s) 290
HhaI GCGC 1 cut(s) 228
Hin1I GRCGYC 1 cut(s) 226
Hin1II CATG 8 cut(s) 120, 184, 353, 604, 682, 847, 927, 945
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 1020
HinfI GANTC 3 cut(s) 137, 760, 920
Hpy166II GTNNAC 2 cut(s) 294, 564
Hpy188I TCNGA 1 cut(s) 134
Hpy188III TCNNGA 4 cut(s) 63, 317, 795, 1034
Hpy8I GTNNAC 2 cut(s) 294, 564
HpyAV CCTTC 2 cut(s) 206, 231
HpyCH4III ACNGT 4 cut(s) 561, 568, 576, 858
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 9 cut(s) 172, 365, 424, 600, 625, 678, 927, 945, 1027
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 74, 371, 1019
HpyF3I CTNAG 2 cut(s) 81, 124
HpySE526I ACGT 1 cut(s) 165
Hsp92I GRCGYC 1 cut(s) 226
Hsp92II CATG 8 cut(s) 120, 184, 353, 604, 682, 847, 927, 945
HspAI GCGC 1 cut(s) 226
KasI GGCGCC 1 cut(s) 225
Kzo9I GATC 5 cut(s) 148, 319, 340, 777, 949
LmnI GCTCC 2 cut(s) 65, 557
Lsp1109I GCAGC 4 cut(s) 36, 64, 85, 358
LweI GCATC 4 cut(s) 64, 374, 808, 964
MaeI CTAG 2 cut(s) 632, 1054
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 1 cut(s) 112
MalI GATC 5 cut(s) 150, 321, 342, 779, 951
MboI GATC 5 cut(s) 148, 319, 340, 777, 949
MboII GAAGA 6 cut(s) 208, 350, 424, 472, 482, 814
MflI RGATCY 1 cut(s) 340
MluCI AATT 9 cut(s) 190, 303, 325, 400, 431, 583, 724, 751, 770
Mly113I GGCGCC 1 cut(s) 226
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 2 cut(s) 637, 1010
MnlI CCTC 8 cut(s) 6, 81, 357, 385, 401, 499, 629, 923
Mph1103I ATGCAT 2 cut(s) 426, 627
MseI TTAA 4 cut(s) 329, 434, 750, 935
MslI CAYNNNNRTG 2 cut(s) 179, 673
MspA1I CMGCKG 1 cut(s) 371
MspR9I CCNGG 1 cut(s) 153
Mva1269I GAATGC 1 cut(s) 128
MvaI CCWGG 1 cut(s) 153
MwoI GCNNNNNNNGC 4 cut(s) 55, 74, 371, 1019
NarI GGCGCC 1 cut(s) 226
NdeII GATC 5 cut(s) 148, 319, 340, 777, 949
NlaIII CATG 8 cut(s) 120, 184, 353, 604, 682, 847, 927, 945
NlaIV GGNNCC 2 cut(s) 227, 543
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 112
NsiI ATGCAT 2 cut(s) 426, 627
NspI RCATGY 3 cut(s) 120, 682, 945
PaeI GCATGC 1 cut(s) 682
PciI ACATGT 1 cut(s) 116
PctI GAATGC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 760, 920
PkrI GCNGC 4 cut(s) 51, 79, 100, 373
PleI GAGTC 1 cut(s) 131
PluTI GGCGCC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 131
PscI ACATGT 1 cut(s) 116
PsiI TTATAA 2 cut(s) 429, 729
Psp1406I AACGTT 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 151
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 2 cut(s) 227, 543
PsuI RGATCY 1 cut(s) 340
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 1 cut(s) 1001
RsaNI GTAC 1 cut(s) 1000
RseI CAYNNNNRTG 2 cut(s) 179, 673
SaqAI TTAA 4 cut(s) 329, 434, 750, 935
SatI GCNGC 4 cut(s) 50, 78, 99, 372
Sau3AI GATC 5 cut(s) 148, 319, 340, 777, 949
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 153
SetI ASST 7 cut(s) 70, 168, 242, 373, 393, 633, 1024
SfaNI GCATC 4 cut(s) 64, 374, 808, 964
SfcI CTRYAG 1 cut(s) 906
SfoI GGCGCC 1 cut(s) 227
SmiMI CAYNNNNRTG 2 cut(s) 179, 673
SphI GCATGC 1 cut(s) 682
Sse9I AATT 9 cut(s) 190, 303, 325, 400, 431, 583, 724, 751, 770
SsiI CCGC 2 cut(s) 278, 1013
SspDI GGCGCC 1 cut(s) 225
SspMI CTAG 2 cut(s) 632, 1054
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 4 cut(s) 561, 568, 576, 858
TaiI ACGT 1 cut(s) 168
TaqI TCGA 3 cut(s) 103, 147, 834
TasI AATT 9 cut(s) 190, 303, 325, 400, 431, 583, 724, 751, 770
TfiI GAWTC 2 cut(s) 760, 920
Tru1I TTAA 4 cut(s) 329, 434, 750, 935
Tru9I TTAA 4 cut(s) 329, 434, 750, 935
TscAI CASTG 4 cut(s) 148, 400, 571, 952
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 4 cut(s) 49, 77, 98, 371
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 5 cut(s) 197, 262, 815, 860, 971
TspRI CASTG 4 cut(s) 148, 400, 571, 952
XapI RAATTY 2 cut(s) 190, 325
XceI RCATGY 3 cut(s) 120, 682, 945
XmiI GTMKAC 1 cut(s) 293
XspI CTAG 2 cut(s) 632, 1054
Zsp2I ATGCAT 2 cut(s) 426, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.