Rroxscaffold_1G00066400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016036
Physical Location & Seq
Forward (+)
87914222 .. 87915956
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00066400.1

Sequence Viewer

Length: 1239 bp
ATGGCCGAGATGAGGATGATGATGATTAGTATGACAATACTAACTTTGCTGCCTTTGCCCTCTGGAGCTTTGGATGTTGCTGAGATGAGGAAAGTTGCTGCTCGAAACAATGTGACATGTATCTTAGCATTCGGAGACTCCAGTGTCGATCCTGGCAATAACAACTTTCTGCACACGACCATGAAAGGGAATTTCCTTCCTTATGGCAAAGACTTCTTCAATGGCCGCCCAACTGGAAGGTTCAGTAATGGCAGACTTGCCACAGATTTCATTGGTAAAACATCAACTTTTCGAGAAAAGATTGAGTTTGAAACCTTCAGTGCAATACCATTTACCACTTCTTTTGTGTCTTTCTGTCTGCATTTTTCCACTTGCACAAGAGAGTCGGTGTCTGTTACGTACCCTGGGCCTGCTGTGCTTCATGAGAAAGTTTTTCCGGACGCAGTTGGGTATACAAAAACAATTCCAGCATTTCTTGATCCAAATTTAAAGCCTGCAGATCTGCCTCATGGTGTTAGTTTTGCATCAGCTGCCTCGGGTTATGACGACCTCACTGCCAATTTTTCGAATGTGTTACCTCTTTCCAGACAGCTGAAGAGTTTTATGCATTATAAATTAAACTTGACAAGATTGGTGGGTAAGAAGACTGCTGTAGATACCATCAAAAATGCCTTATTTGTTATGAGTATGGGAACAAATGACTTTATCCAAAACTACTACTTGGAACCAACTCGCTCCAATCAGTTCACTGTGGAACAGTATCAAAATTATTTGGTCTCTTGCATGGCTCATTCCATTCAGGCAATGCGTAGGCTAGGAGGCACAAGATTGGCTGTTGTTGGAGTTCCACCATTGGGGTGCATGCCACTTGTCAAAACGCTTATGGACGAGACCAAATGTGTGGAAAATTATAACAAAGTGTCATTCTCTTTTAATTCCAAGATTCAAAAGAAATTAGTGAACATTACCAAAACGTCTGGAATGAAGATTGCCTTTGTTGATGCTTATGGTATTGTCGAAAGTGCCATGAATAACCCACAGTTATATGGTCTAACTGAAACTTCAAAGGGGTGTTGTGGGACTGGAACTATAGAGTTTGGAGAATCATGCAGAGGATTAAAAACATGCACTGATCCAGCAAAGTATGTATTCTGGGATGCTGTTCATCCAACAGAGAAAATGTACGAAATCGTTGCTGAGAAAGCTTTGCAAACTCTTGATGGAAAACTCTCTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

412

Amino Acids

45.52

Weight (kDa)

8.77

Isoelectric Point (pI)

28.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 164 - 401 7.8e-25 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45950
fragaria_vesca FvH4_7g02060
malus_domestica MD10G1286100.v1.1
prunus_persica Prupe.4G055800_v2.0.a1
pyrus_communis pycom10g23880
rosa_laevigata RLG00000031700
rosa_roxburghii Rroxscaffold_1G00066400 Rroxscaffold_6G00404910
rosa_rugosa Rorug04G0445700 Rorug04G0445800
rosa_samantha Rh5AG077300 Rh5BG072400 Rh5CG084800 Rh5DG072900
rosa_wichuraiana Rw5G007120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 612, 912
AasI GACNNNNNNGTC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 452
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 1 cut(s) 226
AclWI GGATC 3 cut(s) 143, 473, 1127
AcoI YGGCCR 2 cut(s) 3, 223
AcsI RAATTY 2 cut(s) 190, 484
AcuI CTGAAG 2 cut(s) 301, 614
AfaI GTAC 2 cut(s) 401, 1184
AfiI CCNNNNNNNGG 3 cut(s) 12, 186, 854
AflIII ACRYGT 1 cut(s) 116
AgsI TTSAA 4 cut(s) 220, 311, 947, 1065
AjnI CCWGG 2 cut(s) 151, 403
AluBI AGCT 4 cut(s) 68, 530, 592, 1205
AluI AGCT 4 cut(s) 68, 530, 592, 1205
Alw26I GTCTC 3 cut(s) 129, 781, 884
AlwI GGATC 3 cut(s) 143, 473, 1127
Ama87I CYCGRG 1 cut(s) 535
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 3 cut(s) 3, 223, 407
ApeKI GCWGC 3 cut(s) 49, 98, 530
ApoI RAATTY 2 cut(s) 190, 484
AspS9I GGNCC 1 cut(s) 407
AsuII TTCGAA 1 cut(s) 566
AvaI CYCGRG 1 cut(s) 535
BbsI GAAGAC 1 cut(s) 650
BbvI GCAGC 3 cut(s) 36, 85, 517
BccI CCATC 2 cut(s) 668, 1214
BcgI CGANNNNNNTGC 4 cut(s) 536, 570, 1175, 1209
BciT130I CCWGG 2 cut(s) 153, 405
BcoDI GTCTC 3 cut(s) 129, 781, 884
BfaI CTAG 2 cut(s) 815, 1237
BfmI CTRYAG 3 cut(s) 495, 651, 1089
BglII AGATCT 1 cut(s) 499
BisI GCNGC 4 cut(s) 50, 99, 226, 531
BlsI GCNGC 4 cut(s) 51, 100, 227, 532
Bme1390I CCNGG 2 cut(s) 153, 405
BmeT110I CYCGRG 1 cut(s) 535
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 1 cut(s) 726
BmrFI CCNGG 2 cut(s) 153, 405
BmsI GCATC 3 cut(s) 533, 991, 1147
BpiI GAAGAC 1 cut(s) 650
BpmI CTGGAG 2 cut(s) 84, 124
Bpu14I TTCGAA 1 cut(s) 566
BsaAI YACGTR 1 cut(s) 399
BsaI GGTCTC 2 cut(s) 781, 884
BsaJI CCNNGG 3 cut(s) 403, 404, 534
BsaWI WCCGGW 1 cut(s) 436
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 186, 854
Bse1I ACTGG 3 cut(s) 141, 238, 1087
Bse3DI GCAATG 1 cut(s) 810
BseAI TCCGGA 1 cut(s) 436
BseBI CCWGG 2 cut(s) 153, 405
BseDI CCNNGG 3 cut(s) 403, 404, 534
BseGI GGATG 4 cut(s) 21, 79, 1162, 1165
BseLI CCNNNNNNNGG 3 cut(s) 12, 186, 854
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 2 cut(s) 72, 1188
BseNI ACTGG 3 cut(s) 141, 238, 1087
BseXI GCAGC 3 cut(s) 36, 85, 517
BsgI GTGCAG 1 cut(s) 155
BshFI GGCC 3 cut(s) 5, 225, 409
BsiHKCI CYCGRG 1 cut(s) 535
BsiSI CCGG 1 cut(s) 437
BslFI GGGAC 1 cut(s) 1093
BslI CCNNNNNNNGG 3 cut(s) 12, 186, 854
BsmAI GTCTC 3 cut(s) 129, 781, 884
BsmFI GGGAC 1 cut(s) 1093
BsmI GAATGC 1 cut(s) 128
BsnI GGCC 3 cut(s) 5, 225, 409
Bso31I GGTCTC 2 cut(s) 781, 884
BsoBI CYCGRG 1 cut(s) 535
Bsp119I TTCGAA 1 cut(s) 566
Bsp13I TCCGGA 1 cut(s) 436
Bsp143I GATC 4 cut(s) 148, 478, 499, 1132
BspACI CCGC 1 cut(s) 226
BspANI GGCC 3 cut(s) 5, 225, 409
BspCNI CTCAG 2 cut(s) 73, 1189
BspEI TCCGGA 1 cut(s) 436
BspHI TCATGA 1 cut(s) 421
BspLI GGNNCC 1 cut(s) 726
BspMAI CTGCAG 1 cut(s) 499
BspPI GGATC 3 cut(s) 143, 473, 1127
BspT104I TTCGAA 1 cut(s) 566
BspTNI GGTCTC 2 cut(s) 781, 884
BsrDI GCAATG 1 cut(s) 810
BsrI ACTGG 3 cut(s) 141, 238, 1087
BssECI CCNNGG 3 cut(s) 403, 404, 534
BssMI GATC 4 cut(s) 148, 478, 499, 1132
BssNAI GTATAC 1 cut(s) 453
Bst1107I GTATAC 1 cut(s) 453
Bst2UI CCWGG 2 cut(s) 153, 405
Bst4CI ACNGT 3 cut(s) 751, 759, 1041
Bst6I CTCTTC 1 cut(s) 590
BstAPI GCANNNNNTGC 1 cut(s) 530
BstBAI YACGTR 1 cut(s) 399
BstBI TTCGAA 1 cut(s) 566
BstC8I GCNNGC 3 cut(s) 411, 495, 863
BstDEI CTNAG 3 cut(s) 81, 124, 1197
BstF5I GGATG 4 cut(s) 21, 79, 1162, 1165
BstKTI GATC 4 cut(s) 151, 481, 502, 1135
BstMAI GTCTC 3 cut(s) 129, 781, 884
BstMBI GATC 4 cut(s) 148, 478, 499, 1132
BstMWI GCNNNNNNNGC 4 cut(s) 55, 415, 530, 1202
BstNI CCWGG 2 cut(s) 153, 405
BstNSI RCATGY 3 cut(s) 120, 865, 1128
BstSCI CCNGG 2 cut(s) 151, 403
BstSFI CTRYAG 3 cut(s) 495, 651, 1089
BstSNI TACGTA 1 cut(s) 399
BstV1I GCAGC 3 cut(s) 36, 85, 517
BstV2I GAAGAC 1 cut(s) 650
BstX2I RGATCY 1 cut(s) 499
BstXI CCANNNNNNTGG 1 cut(s) 901
BstYI RGATCY 1 cut(s) 499
BstZ17I GTATAC 1 cut(s) 453
BsuRI GGCC 3 cut(s) 5, 225, 409
BtsCI GGATG 4 cut(s) 21, 79, 1162, 1165
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 5 cut(s) 148, 325, 552, 747, 1128
Cac8I GCNNGC 3 cut(s) 411, 495, 863
CciI TCATGA 1 cut(s) 421
Cfr13I GGNCC 1 cut(s) 407
CseI GACGC 1 cut(s) 449
Csp6I GTAC 2 cut(s) 400, 1183
CspCI CAANNNNNGTGG 4 cut(s) 325, 360, 615, 650
CviAII CATG 9 cut(s) 117, 181, 422, 509, 784, 862, 1027, 1107, 1125
CviQI GTAC 2 cut(s) 400, 1183
DdeI CTNAG 3 cut(s) 81, 124, 1197
DpnI GATC 4 cut(s) 150, 480, 501, 1134
DpnII GATC 4 cut(s) 148, 478, 499, 1132
DraI TTTAAA 1 cut(s) 489
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 2 cut(s) 3, 223
Eam1104I CTCTTC 1 cut(s) 590
EarI CTCTTC 1 cut(s) 590
Eco105I TACGTA 1 cut(s) 399
Eco31I GGTCTC 2 cut(s) 781, 884
Eco57I CTGAAG 2 cut(s) 301, 614
Eco88I CYCGRG 1 cut(s) 535
EcoRII CCWGG 2 cut(s) 151, 403
EcoT22I ATGCAT 1 cut(s) 609
FaeI CATG 9 cut(s) 120, 184, 425, 512, 787, 865, 1030, 1110, 1128
FalI AAGNNNNNCTT 2 cut(s) 977, 1009
FaqI GGGAC 1 cut(s) 1093
FatI CATG 9 cut(s) 116, 180, 421, 508, 783, 861, 1026, 1106, 1124
FblI GTMKAC 1 cut(s) 452
Fnu4HI GCNGC 4 cut(s) 50, 99, 226, 531
FokI GGATG 4 cut(s) 28, 86, 1152, 1169
Fsp4HI GCNGC 4 cut(s) 50, 99, 226, 531
FspBI CTAG 2 cut(s) 815, 1237
GluI GCNGC 4 cut(s) 50, 99, 226, 531
GsuI CTGGAG 2 cut(s) 84, 124
HaeIII GGCC 3 cut(s) 5, 225, 409
HapII CCGG 1 cut(s) 437
HgaI GACGC 1 cut(s) 449
Hin1II CATG 9 cut(s) 120, 184, 425, 512, 787, 865, 1030, 1110, 1128
HindIII AAGCTT 1 cut(s) 1203
HinfI GANTC 4 cut(s) 137, 383, 943, 1103
HpaII CCGG 1 cut(s) 437
Hpy166II GTNNAC 3 cut(s) 453, 747, 961
Hpy188I TCNGA 1 cut(s) 134
Hpy188III TCNNGA 8 cut(s) 63, 293, 422, 437, 476, 585, 978, 1217
Hpy8I GTNNAC 3 cut(s) 453, 747, 961
HpyAV CCTTC 3 cut(s) 206, 231, 325
HpyCH4III ACNGT 3 cut(s) 751, 759, 1041
HpyCH4IV ACGT 2 cut(s) 398, 974
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 415, 530, 1202
HpyF3I CTNAG 3 cut(s) 81, 124, 1197
HpySE526I ACGT 2 cut(s) 398, 974
Hsp92II CATG 9 cut(s) 120, 184, 425, 512, 787, 865, 1030, 1110, 1128
Kpn2I TCCGGA 1 cut(s) 436
Kzo9I GATC 4 cut(s) 148, 478, 499, 1132
LmnI GCTCC 2 cut(s) 65, 740
Lsp1109I GCAGC 3 cut(s) 36, 85, 517
LweI GCATC 3 cut(s) 533, 991, 1147
MaeI CTAG 2 cut(s) 815, 1237
MaeII ACGT 2 cut(s) 398, 974
MaeIII GTNAC 3 cut(s) 112, 394, 573
MalI GATC 4 cut(s) 150, 480, 501, 1134
MboI GATC 4 cut(s) 148, 478, 499, 1132
MboII GAAGA 4 cut(s) 208, 607, 655, 997
MflI RGATCY 1 cut(s) 499
MluCI AATT 9 cut(s) 190, 462, 484, 559, 614, 766, 907, 934, 953
MlyI GAGTC 2 cut(s) 131, 392
MmeI TCCRAC 2 cut(s) 820, 1193
MnlI CCTC 9 cut(s) 6, 70, 81, 516, 544, 560, 588, 812, 1106
Mph1103I ATGCAT 1 cut(s) 609
MroI TCCGGA 1 cut(s) 436
MseI TTAA 4 cut(s) 488, 617, 933, 1118
MslI CAYNNNNRTG 2 cut(s) 179, 856
MspA1I CMGCKG 2 cut(s) 530, 592
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 2 cut(s) 153, 405
Mva1269I GAATGC 1 cut(s) 128
MvaI CCWGG 2 cut(s) 153, 405
MwoI GCNNNNNNNGC 4 cut(s) 55, 415, 530, 1202
NdeII GATC 4 cut(s) 148, 478, 499, 1132
NlaIII CATG 9 cut(s) 120, 184, 425, 512, 787, 865, 1030, 1110, 1128
NlaIV GGNNCC 1 cut(s) 726
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 112
NsiI ATGCAT 1 cut(s) 609
NspI RCATGY 3 cut(s) 120, 865, 1128
NspV TTCGAA 1 cut(s) 566
PaeI GCATGC 1 cut(s) 865
PagI TCATGA 1 cut(s) 421
PasI CCCWGGG 1 cut(s) 404
PciI ACATGT 1 cut(s) 116
PctI GAATGC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 943, 1103
PkrI GCNGC 4 cut(s) 51, 100, 227, 532
PleI GAGTC 2 cut(s) 131, 391
PpsI GAGTC 2 cut(s) 131, 391
Ppu21I YACGTR 1 cut(s) 399
PscI ACATGT 1 cut(s) 116
PsiI TTATAA 2 cut(s) 612, 912
Psp6I CCWGG 2 cut(s) 151, 403
PspGI CCWGG 2 cut(s) 151, 403
PspN4I GGNNCC 1 cut(s) 726
PspPI GGNCC 1 cut(s) 407
PstI CTGCAG 1 cut(s) 499
PsuI RGATCY 1 cut(s) 499
PvuII CAGCTG 2 cut(s) 530, 592
RsaI GTAC 2 cut(s) 401, 1184
RsaNI GTAC 2 cut(s) 400, 1183
RseI CAYNNNNRTG 2 cut(s) 179, 856
SaqAI TTAA 4 cut(s) 488, 617, 933, 1118
SatI GCNGC 4 cut(s) 50, 99, 226, 531
Sau3AI GATC 4 cut(s) 148, 478, 499, 1132
Sau96I GGNCC 1 cut(s) 407
SchI GAGTC 2 cut(s) 131, 392
ScrFI CCNGG 2 cut(s) 153, 405
SfaNI GCATC 3 cut(s) 533, 991, 1147
SfcI CTRYAG 3 cut(s) 495, 651, 1089
SfuI TTCGAA 1 cut(s) 566
SmiMI CAYNNNNRTG 2 cut(s) 179, 856
SnaBI TACGTA 1 cut(s) 399
SphI GCATGC 1 cut(s) 865
Sse9I AATT 9 cut(s) 190, 462, 484, 559, 614, 766, 907, 934, 953
SsiI CCGC 1 cut(s) 226
SspMI CTAG 2 cut(s) 815, 1237
StyD4I CCNGG 2 cut(s) 151, 403
TaaI ACNGT 3 cut(s) 751, 759, 1041
TaiI ACGT 2 cut(s) 401, 977
TaqI TCGA 5 cut(s) 103, 147, 292, 566, 1017
TasI AATT 9 cut(s) 190, 462, 484, 559, 614, 766, 907, 934, 953
TauI GCSGC 1 cut(s) 228
TfiI GAWTC 2 cut(s) 943, 1103
Tru1I TTAA 4 cut(s) 488, 617, 933, 1118
Tru9I TTAA 4 cut(s) 488, 617, 933, 1118
TscAI CASTG 5 cut(s) 148, 325, 559, 754, 1135
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 3 cut(s) 49, 98, 530
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 6 cut(s) 197, 259, 410, 998, 1043, 1154
TspRI CASTG 5 cut(s) 148, 325, 559, 754, 1135
XapI RAATTY 2 cut(s) 190, 484
XceI RCATGY 3 cut(s) 120, 865, 1128
XmiI GTMKAC 1 cut(s) 452
XspI CTAG 2 cut(s) 815, 1237
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.