Rh5BG072400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016036
Physical Location & Seq
Reverse (-)
5959425 .. 5961725
2301 bp
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UTR
Exon/CDS
Intron
Rh5BG072400.1

Sequence Viewer

Length: 660 bp
ATGGCCGAAATGAGGATGATGATGATTAGTATGACAATACTAACTTTGCTGCCTTTGCTCTCTGGAGCTTTGGATGTTGCTGAGATGAGGAAAGTCGCTGCTCGAAACAATGTGACATGTATCTTAGTATTCGGAGACTCCAGTGTCGATCCTGGCAATAACAACGTTCTGCACACGACCATGAAAGGGAATTTCCTTCCTTATGGCAAAGAATTCTTCAATGGCCGCCCAACTGGAAGATTCAGTAATGGGAGACTTGCCACAGATTTTATTGCGGACGCAGTTGGGTATACAAAAACAATTCCAGCATTTCTTGATCCAAATTTAAAGCCTGCAGATCTGCCTCATGGTGTTAGTTTTGCATCAGCTGCCTCGGGTTATGACGACCTCACTGCCAATTTTTCGGCAATGCATAGGCTAGGAGGCACAAGATTGGCTGTTGTTGGAGTTCCACCATTGGGGTGCATGCCACTTGTCAAAACGCTTATGGGCGAGACCAAATGTGTGGAAAATTATAACGAAGTGTCATTCTCTTTTAATTCCAAGATTCAAAAGAAATTAGAGAACATTACCAAAACATCTGGAATGAAGATTGCCTTTGTTGATGCATATGGTATTGTCGAAAGTGCCATGAATAACCCACAGTTATATGGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

219

Amino Acids

23.75

Weight (kDa)

8.4

Isoelectric Point (pI)

16.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 128 - 212 2.6e-06 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45950
fragaria_vesca FvH4_7g02060
malus_domestica MD10G1286100.v1.1
prunus_persica Prupe.4G055800_v2.0.a1
pyrus_communis pycom10g23880
rosa_laevigata RLG00000031700
rosa_roxburghii Rroxscaffold_1G00066400 Rroxscaffold_6G00404910
rosa_rugosa Rorug04G0445700 Rorug04G0445800
rosa_samantha Rh5AG077300 Rh5BG072400 Rh5CG084800 Rh5DG072900
rosa_wichuraiana Rw5G007120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 516
AasI GACNNNNNNGTC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 290
AciI CCGC 2 cut(s) 226, 275
AclI AACGTT 1 cut(s) 165
AclWI GGATC 2 cut(s) 143, 311
AcoI YGGCCR 2 cut(s) 3, 223
AcsI RAATTY 3 cut(s) 190, 212, 322
AfiI CCNNNNNNNGG 3 cut(s) 12, 186, 458
AflIII ACRYGT 1 cut(s) 116
AgsI TTSAA 2 cut(s) 220, 551
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 2 cut(s) 68, 368
AluI AGCT 2 cut(s) 68, 368
Alw26I GTCTC 3 cut(s) 129, 247, 488
AlwI GGATC 2 cut(s) 143, 311
Ama87I CYCGRG 1 cut(s) 373
AoxI GGCC 2 cut(s) 3, 223
ApeKI GCWGC 3 cut(s) 49, 98, 368
ApoI RAATTY 3 cut(s) 190, 212, 322
AvaI CYCGRG 1 cut(s) 373
BbvI GCAGC 3 cut(s) 36, 85, 355
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 1 cut(s) 153
BcoDI GTCTC 3 cut(s) 129, 247, 488
BfaI CTAG 1 cut(s) 419
BfmI CTRYAG 1 cut(s) 333
BglII AGATCT 1 cut(s) 337
BisI GCNGC 4 cut(s) 50, 99, 226, 369
BlsI GCNGC 4 cut(s) 51, 100, 227, 370
Bme1390I CCNGG 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 373
BmrFI CCNGG 1 cut(s) 153
BmsI GCATC 2 cut(s) 371, 595
BpmI CTGGAG 2 cut(s) 84, 124
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 1 cut(s) 372
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 186, 458
Bse1I ACTGG 2 cut(s) 141, 238
Bse3DI GCAATG 1 cut(s) 414
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 2 cut(s) 21, 79
BseLI CCNNNNNNNGG 3 cut(s) 12, 186, 458
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 2 cut(s) 141, 238
BseXI GCAGC 3 cut(s) 36, 85, 355
BsgI GTGCAG 1 cut(s) 155
BshFI GGCC 2 cut(s) 5, 225
BsiHKCI CYCGRG 1 cut(s) 373
BslI CCNNNNNNNGG 3 cut(s) 12, 186, 458
BsmAI GTCTC 3 cut(s) 129, 247, 488
BsnI GGCC 2 cut(s) 5, 225
Bso31I GGTCTC 1 cut(s) 488
BsoBI CYCGRG 1 cut(s) 373
Bsp143I GATC 3 cut(s) 148, 316, 337
BspACI CCGC 2 cut(s) 226, 275
BspANI GGCC 2 cut(s) 5, 225
BspCNI CTCAG 1 cut(s) 73
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 2 cut(s) 143, 311
BspTNI GGTCTC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 414
BsrI ACTGG 2 cut(s) 141, 238
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 3 cut(s) 148, 316, 337
BssNAI GTATAC 1 cut(s) 291
Bst1107I GTATAC 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 1 cut(s) 645
BstAPI GCANNNNNTGC 1 cut(s) 368
BstC8I GCNNGC 2 cut(s) 333, 467
BstDEI CTNAG 2 cut(s) 81, 124
BstF5I GGATG 2 cut(s) 21, 79
BstKTI GATC 3 cut(s) 151, 319, 340
BstMAI GTCTC 3 cut(s) 129, 247, 488
BstMBI GATC 3 cut(s) 148, 316, 337
BstMWI GCNNNNNNNGC 2 cut(s) 55, 368
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 2 cut(s) 120, 469
BstSCI CCNGG 1 cut(s) 151
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 3 cut(s) 36, 85, 355
BstX2I RGATCY 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 505
BstYI RGATCY 1 cut(s) 337
BstZ17I GTATAC 1 cut(s) 291
BsuRI GGCC 2 cut(s) 5, 225
BtsCI GGATG 2 cut(s) 21, 79
BtsI GCAGTG 1 cut(s) 390
BtsIMutI CAGTG 2 cut(s) 148, 390
Cac8I GCNNGC 2 cut(s) 333, 467
CseI GACGC 1 cut(s) 287
CviAII CATG 5 cut(s) 117, 181, 347, 466, 631
CviJI RGCY 7 cut(s) 5, 68, 225, 331, 368, 418, 437
CviKI_1 RGCY 7 cut(s) 5, 68, 225, 331, 368, 418, 437
DdeI CTNAG 2 cut(s) 81, 124
DpnI GATC 3 cut(s) 150, 318, 339
DpnII GATC 3 cut(s) 148, 316, 337
DraI TTTAAA 1 cut(s) 327
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 2 cut(s) 3, 223
Eco31I GGTCTC 1 cut(s) 488
Eco88I CYCGRG 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 151
EcoT22I ATGCAT 2 cut(s) 414, 610
FaeI CATG 5 cut(s) 120, 184, 350, 469, 634
FalI AAGNNNNNCTT 2 cut(s) 581, 613
FatI CATG 5 cut(s) 116, 180, 346, 465, 630
FauNDI CATATG 1 cut(s) 610
FblI GTMKAC 1 cut(s) 290
Fnu4HI GCNGC 4 cut(s) 50, 99, 226, 369
FokI GGATG 2 cut(s) 28, 86
Fsp4HI GCNGC 4 cut(s) 50, 99, 226, 369
FspBI CTAG 1 cut(s) 419
GluI GCNGC 4 cut(s) 50, 99, 226, 369
GsuI CTGGAG 2 cut(s) 84, 124
HaeIII GGCC 2 cut(s) 5, 225
HgaI GACGC 1 cut(s) 287
Hin1II CATG 5 cut(s) 120, 184, 350, 469, 634
HinfI GANTC 3 cut(s) 137, 240, 547
Hpy166II GTNNAC 1 cut(s) 291
Hpy188I TCNGA 1 cut(s) 134
Hpy188III TCNNGA 3 cut(s) 63, 314, 582
Hpy8I GTNNAC 1 cut(s) 291
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 1 cut(s) 645
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 6 cut(s) 172, 335, 362, 412, 465, 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 368
HpyF3I CTNAG 2 cut(s) 81, 124
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 5 cut(s) 120, 184, 350, 469, 634
Kzo9I GATC 3 cut(s) 148, 316, 337
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 8 cut(s) 48, 138, 154, 165, 219, 318, 345, 567
Lsp1109I GCAGC 3 cut(s) 36, 85, 355
LweI GCATC 2 cut(s) 371, 595
MaeI CTAG 1 cut(s) 419
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 1 cut(s) 112
MalI GATC 3 cut(s) 150, 318, 339
MboI GATC 3 cut(s) 148, 316, 337
MboII GAAGA 3 cut(s) 208, 249, 601
MflI RGATCY 1 cut(s) 337
MluCI AATT 8 cut(s) 190, 212, 300, 322, 397, 511, 538, 557
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 6 cut(s) 6, 81, 354, 382, 398, 416
Mph1103I ATGCAT 2 cut(s) 414, 610
MseI TTAA 2 cut(s) 326, 537
MslI CAYNNNNRTG 2 cut(s) 179, 460
MspA1I CMGCKG 1 cut(s) 368
MspR9I CCNGG 1 cut(s) 153
MvaI CCWGG 1 cut(s) 153
MwoI GCNNNNNNNGC 2 cut(s) 55, 368
NdeI CATATG 1 cut(s) 610
NdeII GATC 3 cut(s) 148, 316, 337
NlaIII CATG 5 cut(s) 120, 184, 350, 469, 634
NmuCI GTSAC 1 cut(s) 112
NsiI ATGCAT 2 cut(s) 414, 610
NspI RCATGY 2 cut(s) 120, 469
PaeI GCATGC 1 cut(s) 469
PciI ACATGT 1 cut(s) 116
PfeI GAWTC 2 cut(s) 240, 547
PkrI GCNGC 4 cut(s) 51, 100, 227, 370
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PscI ACATGT 1 cut(s) 116
PsiI TTATAA 1 cut(s) 516
Psp1406I AACGTT 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 151
PspGI CCWGG 1 cut(s) 151
PstI CTGCAG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 368
RseI CAYNNNNRTG 2 cut(s) 179, 460
SaqAI TTAA 2 cut(s) 326, 537
SatI GCNGC 4 cut(s) 50, 99, 226, 369
Sau3AI GATC 3 cut(s) 148, 316, 337
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 153
SetI ASST 4 cut(s) 70, 168, 370, 390
SfaNI GCATC 2 cut(s) 371, 595
SfcI CTRYAG 1 cut(s) 333
SmiMI CAYNNNNRTG 2 cut(s) 179, 460
SphI GCATGC 1 cut(s) 469
Sse9I AATT 8 cut(s) 190, 212, 300, 322, 397, 511, 538, 557
SsiI CCGC 2 cut(s) 226, 275
SspMI CTAG 1 cut(s) 419
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 645
TaiI ACGT 1 cut(s) 168
TaqI TCGA 3 cut(s) 103, 147, 621
TasI AATT 8 cut(s) 190, 212, 300, 322, 397, 511, 538, 557
TauI GCSGC 1 cut(s) 228
TfiI GAWTC 2 cut(s) 240, 547
Tru1I TTAA 2 cut(s) 326, 537
Tru9I TTAA 2 cut(s) 326, 537
TscAI CASTG 2 cut(s) 148, 397
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 3 cut(s) 49, 98, 368
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 3 cut(s) 197, 602, 647
TspRI CASTG 2 cut(s) 148, 397
XapI RAATTY 3 cut(s) 190, 212, 322
XceI RCATGY 2 cut(s) 120, 469
XmiI GTMKAC 1 cut(s) 290
XspI CTAG 1 cut(s) 419
Zsp2I ATGCAT 2 cut(s) 414, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.