RLG00000031700

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016036
Physical Location & Seq
Reverse (-)
7565849 .. 7571154
5306 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031700

Sequence Viewer

Length: 1222 bp
ATGGCCGAGATGAGGATGATGAAGATTAGTATGACAATACTAACTTTGCTGCCTTTGCTCTCTGGAGCTTTGGATGTTGCTGAGATGAGGAAAGTTGCTGCTCGAAACAATGTGACATGTATCTTAGCATTCGGAGACTCCAGTGTCGATCCTGGCAATAACAACGTTCTGCACACGAACATGAAAGGGAATTTCCTTCCTTATGGCAAAGACTTCTTCAATGGCCGCCCAACTGGAAGGTTCAGTAATGGCAGACTTGCCACAGATTTCATTGCGGACGCAGTTGGGTATACAAAAACAATTCCAGCATTTCTTGACCCAAATTTAAAGCCTGCAGATCTGCCTCATGGTGTTAGTTTTGCATCAGCTGCCTCGGGTTATGACGACCTCACTGCCAATTTTTCGAATGTGTTACCTCTTTCCAGACAGCTGAAGAGTTTTATGCATTATAAATTAAACTTGACAAGATTGATGGGTAAGAAGACTGCTGAAGATACCATCAAAAATGCCTTATTTGTTATGAGTATGGGAACAAATGACTTTATCCAAAACTACTACTTGGAACCAACTCGCTCCAAGCAGTTCACTGTGGAACAGTATCAAAATTATTTGCTCTGTTGCATGGCTCATTCTATTCAGACTGAAGGAAAGACTGTTGAAGCCAAGAAGCCTATTGTTGTGAAGTACGGTCTCAACCATGTCACCTACCTCATTGAGCAGAACAAGGCCCATCTTGTGGTTATCGCTCATGATGTCGATCCAATTGAGTTGGTTGTATGGCTCCCTGCTTTATGCAAAAAGATGGAGATCCCCTATTGCATTGTGAAGGGCAAAGCACGTGTAGGAGCGCTGCCTCTCTATTACCGGCCGCCCATGCCTCTTCTTCCTCTCAATCTTTCCGATTCCCTAAACTCCAAAGCTTTCCTTTCTTTCCTCCTCCTCCCCAAACCCAAATCTTTTGTTCCTCGCTACAAGCCTTACCCACTCTCCCTCAGCTCTCCACATGAACGCTCCCACATTGGAACGGAAGCCCTCCTTTCAGGTCTCACTGCAATTCTCACACCCTTCTTTAATAATGTTGTATTGCCAGAGCTACTGACAGAGTACATGGCAGATATGAAATGTGAGGGTTGTGTTAAGTCTTTCAAGAAAATGCTACAAGTTGTTGATGGGGTTAAAAGTGTAGAGGCGGACCTCAACACCCAAGTCGTTAGGATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

407

Amino Acids

45.32

Weight (kDa)

9.1

Isoelectric Point (pI)

29.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 195 1.2e-12 GDSL-like Lipase/Acylhydrolase
Ribosomal_L7Ae PF01248 215 - 284 1.2e-20 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
HMA PF00403 370 - 406 7.7e-07 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45950
fragaria_vesca FvH4_7g02060
malus_domestica MD10G1286100.v1.1
prunus_persica Prupe.4G055800_v2.0.a1
pyrus_communis pycom10g23880
rosa_laevigata RLG00000031700
rosa_roxburghii Rroxscaffold_1G00066400 Rroxscaffold_6G00404910
rosa_rugosa Rorug04G0445700 Rorug04G0445800
rosa_samantha Rh5AG077300 Rh5BG072400 Rh5CG084800 Rh5DG072900
rosa_wichuraiana Rw5G007120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
AasI GACNNNNNNGTC 1 cut(s) 143
AccB7I CCANNNNNTGG 1 cut(s) 736
AccI GTMKAC 1 cut(s) 290
AciI CCGC 4 cut(s) 226, 275, 869, 1190
AclI AACGTT 1 cut(s) 165
AclWI GGATC 3 cut(s) 143, 752, 802
AcoI YGGCCR 3 cut(s) 3, 223, 866
AcsI RAATTY 2 cut(s) 190, 322
AcuI CTGAAG 3 cut(s) 452, 510, 663
AcvI CACGTG 1 cut(s) 839
AfaI GTAC 2 cut(s) 686, 1106
AfeI AGCGCT 1 cut(s) 849
AfiI CCNNNNNNNGG 2 cut(s) 12, 736
AflIII ACRYGT 2 cut(s) 116, 838
AgsI TTSAA 3 cut(s) 220, 659, 1147
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 6 cut(s) 68, 368, 430, 920, 996, 1093
AluI AGCT 6 cut(s) 68, 368, 430, 920, 996, 1093
Alw26I GTCTC 3 cut(s) 129, 695, 1049
AlwI GGATC 3 cut(s) 143, 752, 802
Ama87I CYCGRG 1 cut(s) 373
Aor51HI AGCGCT 1 cut(s) 849
AoxI GGCC 4 cut(s) 3, 223, 726, 866
ApeKI GCWGC 4 cut(s) 49, 98, 368, 850
ApoI RAATTY 2 cut(s) 190, 322
AspLEI GCGC 1 cut(s) 850
AspS9I GGNCC 2 cut(s) 727, 1192
AsuHPI GGTGA 1 cut(s) 694
AsuII TTCGAA 1 cut(s) 404
AvaI CYCGRG 1 cut(s) 373
AvaII GGWCC 1 cut(s) 1192
BbrPI CACGTG 1 cut(s) 839
BbsI GAAGAC 1 cut(s) 488
BbvCI CCTCAGC 1 cut(s) 992
BbvI GCAGC 4 cut(s) 36, 85, 355, 837
BccI CCATC 5 cut(s) 466, 506, 738, 796, 1163
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 1 cut(s) 153
BcoDI GTCTC 3 cut(s) 129, 695, 1049
BfaI CTAG 1 cut(s) 1220
BfmI CTRYAG 1 cut(s) 333
BfoI RGCGCY 1 cut(s) 851
BglII AGATCT 1 cut(s) 337
BisI GCNGC 6 cut(s) 50, 99, 226, 369, 851, 869
BlsI GCNGC 6 cut(s) 51, 100, 227, 370, 852, 870
Bme1390I CCNGG 1 cut(s) 153
Bme18I GGWCC 1 cut(s) 1192
BmeT110I CYCGRG 1 cut(s) 373
BmgT120I GGNCC 2 cut(s) 727, 1192
BmiI GGNNCC 2 cut(s) 564, 782
BmrFI CCNGG 1 cut(s) 153
BmsI GCATC 1 cut(s) 371
BpiI GAAGAC 1 cut(s) 488
BpmI CTGGAG 2 cut(s) 84, 124
Bpu10I CCTNAGC 1 cut(s) 992
Bpu14I TTCGAA 1 cut(s) 404
BsaAI YACGTR 1 cut(s) 839
BsaBI GATNNNNATC 2 cut(s) 756, 806
BsaI GGTCTC 2 cut(s) 695, 1049
BsaJI CCNNGG 1 cut(s) 372
BsaXI ACNNNNNCTCC 2 cut(s) 971, 1001
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 736
Bse118I RCCGGY 1 cut(s) 864
Bse1I ACTGG 2 cut(s) 141, 238
Bse3DI GCAATG 1 cut(s) 270
Bse8I GATNNNNATC 2 cut(s) 756, 806
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 2 cut(s) 21, 79
BseJI GATNNNNATC 2 cut(s) 756, 806
BseLI CCNNNNNNNGG 2 cut(s) 12, 736
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 2 cut(s) 72, 1006
BseNI ACTGG 2 cut(s) 141, 238
BseRI GAGGAG 2 cut(s) 926, 929
BseX3I CGGCCG 1 cut(s) 866
BseXI GCAGC 4 cut(s) 36, 85, 355, 837
BsgI GTGCAG 1 cut(s) 155
Bsh1285I CGRYCG 1 cut(s) 869
BshFI GGCC 4 cut(s) 5, 225, 728, 868
BsiEI CGRYCG 1 cut(s) 869
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 1 cut(s) 865
BslI CCNNNNNNNGG 2 cut(s) 12, 736
BsmAI GTCTC 3 cut(s) 129, 695, 1049
BsmI GAATGC 1 cut(s) 128
BsnI GGCC 4 cut(s) 5, 225, 728, 868
Bso31I GGTCTC 2 cut(s) 695, 1049
BsoBI CYCGRG 1 cut(s) 373
Bsp119I TTCGAA 1 cut(s) 404
Bsp143I GATC 4 cut(s) 148, 337, 757, 807
BspACI CCGC 4 cut(s) 226, 275, 869, 1190
BspANI GGCC 4 cut(s) 5, 225, 728, 868
BspCNI CTCAG 2 cut(s) 73, 1005
BspHI TCATGA 1 cut(s) 748
BspLI GGNNCC 2 cut(s) 564, 782
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 3 cut(s) 143, 752, 802
BspT104I TTCGAA 1 cut(s) 404
BspTNI GGTCTC 2 cut(s) 695, 1049
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 864
BsrI ACTGG 2 cut(s) 141, 238
BssAI RCCGGY 1 cut(s) 864
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 4 cut(s) 148, 337, 757, 807
BssNAI GTATAC 1 cut(s) 291
Bst1107I GTATAC 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 4 cut(s) 589, 597, 655, 689
Bst6I CTCTTC 2 cut(s) 428, 885
BstAPI GCANNNNNTGC 1 cut(s) 368
BstBAI YACGTR 1 cut(s) 839
BstBI TTCGAA 1 cut(s) 404
BstC8I GCNNGC 1 cut(s) 333
BstDEI CTNAG 3 cut(s) 81, 124, 992
BstF5I GGATG 2 cut(s) 21, 79
BstH2I RGCGCY 1 cut(s) 851
BstHHI GCGC 1 cut(s) 850
BstKTI GATC 4 cut(s) 151, 340, 760, 810
BstMAI GTCTC 3 cut(s) 129, 695, 1049
BstMBI GATC 4 cut(s) 148, 337, 757, 807
BstMCI CGRYCG 1 cut(s) 869
BstMWI GCNNNNNNNGC 3 cut(s) 55, 368, 874
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 1 cut(s) 120
BstSCI CCNGG 1 cut(s) 151
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 4 cut(s) 36, 85, 355, 837
BstV2I GAAGAC 1 cut(s) 488
BstX2I RGATCY 2 cut(s) 337, 807
BstYI RGATCY 2 cut(s) 337, 807
BstZ17I GTATAC 1 cut(s) 291
BstZI CGGCCG 1 cut(s) 866
BsuRI GGCC 4 cut(s) 5, 225, 728, 868
BtsCI GGATG 2 cut(s) 21, 79
BtsI GCAGTG 2 cut(s) 390, 1047
BtsIMutI CAGTG 4 cut(s) 148, 390, 585, 1047
Cac8I GCNNGC 1 cut(s) 333
CciI TCATGA 1 cut(s) 748
CfoI GCGC 1 cut(s) 850
Cfr10I RCCGGY 1 cut(s) 864
Cfr13I GGNCC 2 cut(s) 727, 1192
CseI GACGC 1 cut(s) 287
Csp6I GTAC 2 cut(s) 685, 1105
CviAII CATG 9 cut(s) 117, 181, 347, 622, 698, 749, 874, 1004, 1108
CviQI GTAC 2 cut(s) 685, 1105
DdeI CTNAG 3 cut(s) 81, 124, 992
DpnI GATC 4 cut(s) 150, 339, 759, 809
DpnII GATC 4 cut(s) 148, 337, 757, 807
DraI TTTAAA 1 cut(s) 327
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 3 cut(s) 3, 223, 866
EagI CGGCCG 1 cut(s) 866
Eam1104I CTCTTC 2 cut(s) 428, 885
EarI CTCTTC 2 cut(s) 428, 885
EciI GGCGGA 1 cut(s) 1205
EclXI CGGCCG 1 cut(s) 866
Eco31I GGTCTC 2 cut(s) 695, 1049
Eco47I GGWCC 1 cut(s) 1192
Eco47III AGCGCT 1 cut(s) 849
Eco52I CGGCCG 1 cut(s) 866
Eco57I CTGAAG 3 cut(s) 452, 510, 663
Eco72I CACGTG 1 cut(s) 839
Eco88I CYCGRG 1 cut(s) 373
EcoRII CCWGG 1 cut(s) 151
EcoT22I ATGCAT 1 cut(s) 447
FaeI CATG 9 cut(s) 120, 184, 350, 625, 701, 752, 877, 1007, 1111
FalI AAGNNNNNCTT 4 cut(s) 909, 941, 1020, 1052
FatI CATG 9 cut(s) 116, 180, 346, 621, 697, 748, 873, 1003, 1107
FblI GTMKAC 1 cut(s) 290
Fnu4HI GCNGC 6 cut(s) 50, 99, 226, 369, 851, 869
FokI GGATG 2 cut(s) 28, 86
Fsp4HI GCNGC 6 cut(s) 50, 99, 226, 369, 851, 869
FspBI CTAG 1 cut(s) 1220
GlaI GCGC 1 cut(s) 849
GluI GCNGC 6 cut(s) 50, 99, 226, 369, 851, 869
GsuI CTGGAG 2 cut(s) 84, 124
HaeII RGCGCY 1 cut(s) 851
HaeIII GGCC 4 cut(s) 5, 225, 728, 868
HapII CCGG 1 cut(s) 865
HgaI GACGC 1 cut(s) 287
HhaI GCGC 1 cut(s) 850
Hin1II CATG 9 cut(s) 120, 184, 350, 625, 701, 752, 877, 1007, 1111
Hin6I GCGC 1 cut(s) 848
HinP1I GCGC 1 cut(s) 848
HindIII AAGCTT 1 cut(s) 918
HinfI GANTC 3 cut(s) 137, 902, 1216
HpaII CCGG 1 cut(s) 865
HphI GGTGA 1 cut(s) 694
Hpy166II GTNNAC 2 cut(s) 291, 585
Hpy188I TCNGA 3 cut(s) 134, 639, 901
Hpy188III TCNNGA 5 cut(s) 63, 314, 423, 749, 1147
Hpy8I GTNNAC 2 cut(s) 291, 585
HpyAV CCTTC 5 cut(s) 206, 231, 638, 820, 1075
HpyCH4III ACNGT 4 cut(s) 589, 597, 655, 689
HpyCH4IV ACGT 2 cut(s) 165, 838
HpyCH4V TGCA 8 cut(s) 172, 335, 362, 445, 621, 795, 819, 1052
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 368, 874
HpyF3I CTNAG 3 cut(s) 81, 124, 992
HpySE526I ACGT 2 cut(s) 165, 838
Hsp92II CATG 9 cut(s) 120, 184, 350, 625, 701, 752, 877, 1007, 1111
HspAI GCGC 1 cut(s) 848
Kzo9I GATC 4 cut(s) 148, 337, 757, 807
LmnI GCTCC 5 cut(s) 65, 578, 786, 845, 1016
Lsp1109I GCAGC 4 cut(s) 36, 85, 355, 837
LweI GCATC 1 cut(s) 371
MaeI CTAG 1 cut(s) 1220
MaeII ACGT 2 cut(s) 165, 838
MaeIII GTNAC 3 cut(s) 112, 411, 700
MalI GATC 4 cut(s) 150, 339, 759, 809
MboI GATC 4 cut(s) 148, 337, 757, 807
MboII GAAGA 7 cut(s) 34, 208, 445, 493, 503, 872, 875
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 2 cut(s) 337, 807
MluCI AATT 8 cut(s) 190, 300, 322, 397, 452, 604, 762, 1053
MlyI GAGTC 1 cut(s) 131
Mph1103I ATGCAT 1 cut(s) 447
MseI TTAA 5 cut(s) 326, 455, 1071, 1137, 1176
MslI CAYNNNNRTG 1 cut(s) 179
MspA1I CMGCKG 2 cut(s) 368, 430
MspI CCGG 1 cut(s) 865
MspR9I CCNGG 1 cut(s) 153
MunI CAATTG 1 cut(s) 762
Mva1269I GAATGC 1 cut(s) 128
MvaI CCWGG 1 cut(s) 153
MwoI GCNNNNNNNGC 3 cut(s) 55, 368, 874
NdeII GATC 4 cut(s) 148, 337, 757, 807
NlaIII CATG 9 cut(s) 120, 184, 350, 625, 701, 752, 877, 1007, 1111
NlaIV GGNNCC 2 cut(s) 564, 782
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 2 cut(s) 112, 700
NsiI ATGCAT 1 cut(s) 447
NspI RCATGY 1 cut(s) 120
NspV TTCGAA 1 cut(s) 404
PagI TCATGA 1 cut(s) 748
PciI ACATGT 1 cut(s) 116
PctI GAATGC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 902, 1216
PflMI CCANNNNNTGG 1 cut(s) 736
PkrI GCNGC 6 cut(s) 51, 100, 227, 370, 852, 870
PleI GAGTC 1 cut(s) 131
PmaCI CACGTG 1 cut(s) 839
PmlI CACGTG 1 cut(s) 839
PpsI GAGTC 1 cut(s) 131
Ppu21I YACGTR 1 cut(s) 839
PscI ACATGT 1 cut(s) 116
PsiI TTATAA 1 cut(s) 450
Psp1406I AACGTT 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 151
PspCI CACGTG 1 cut(s) 839
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 2 cut(s) 564, 782
PspPI GGNCC 2 cut(s) 727, 1192
PstI CTGCAG 1 cut(s) 337
PsuI RGATCY 2 cut(s) 337, 807
PvuII CAGCTG 2 cut(s) 368, 430
RsaI GTAC 2 cut(s) 686, 1106
RsaNI GTAC 2 cut(s) 685, 1105
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 5 cut(s) 326, 455, 1071, 1137, 1176
SatI GCNGC 6 cut(s) 50, 99, 226, 369, 851, 869
Sau3AI GATC 4 cut(s) 148, 337, 757, 807
Sau96I GGNCC 2 cut(s) 727, 1192
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 153
SfaNI GCATC 1 cut(s) 371
SfcI CTRYAG 1 cut(s) 333
SfuI TTCGAA 1 cut(s) 404
SinI GGWCC 1 cut(s) 1192
SmiMI CAYNNNNRTG 1 cut(s) 179
Sse9I AATT 8 cut(s) 190, 300, 322, 397, 452, 604, 762, 1053
SsiI CCGC 4 cut(s) 226, 275, 869, 1190
SspMI CTAG 1 cut(s) 1220
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 4 cut(s) 589, 597, 655, 689
TaiI ACGT 2 cut(s) 168, 841
TaqI TCGA 4 cut(s) 103, 147, 404, 756
TasI AATT 8 cut(s) 190, 300, 322, 397, 452, 604, 762, 1053
TatI WGTACW 1 cut(s) 1104
TauI GCSGC 2 cut(s) 228, 871
TfiI GAWTC 2 cut(s) 902, 1216
Tru1I TTAA 5 cut(s) 326, 455, 1071, 1137, 1176
Tru9I TTAA 5 cut(s) 326, 455, 1071, 1137, 1176
TscAI CASTG 4 cut(s) 148, 397, 592, 1054
TseFI GTSAC 2 cut(s) 112, 700
TseI GCWGC 4 cut(s) 49, 98, 368, 850
Tsp45I GTSAC 2 cut(s) 112, 700
TspDTI ATGAA 5 cut(s) 35, 197, 259, 1020, 1133
TspGWI ACGGA 1 cut(s) 1040
TspRI CASTG 4 cut(s) 148, 397, 592, 1054
Van91I CCANNNNNTGG 1 cut(s) 736
VpaK11BI GGWCC 1 cut(s) 1192
XapI RAATTY 2 cut(s) 190, 322
XceI RCATGY 1 cut(s) 120
XmiI GTMKAC 1 cut(s) 290
XspI CTAG 1 cut(s) 1220
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.