AT5G46530

AWPM-19-like membrane family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009983
Physical Location & Seq
Forward (+)
18875382 .. 18876928
1547 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46530.1

Sequence Viewer

Length: 474 bp
ATGGTGGAACATCAGATGAAACCGGTGGCCTCTTTTCTTTTAGTCCTGAACTTTTGTATGTATGCCATTGTTCTTGGAATTGGCGCATGGTCCATGAACAAAGCTATCAACCATGGTTTCCTTATTGGTGCGGATTATAGTCTTCCGGCTCATTTCTCGCCGATACATTTTCCAATGGGTAATGCGGCCACCGGATTCTTCATTATGTTTGCTTTAATTGCTGGAGTTGCTGGAGCTGCCTCGGTTATCTCCGGCATCAGTCATCTCCAATCATGGACAACGACTAGTCTCCCGGCAGCTGTCTCTGCCGCCACTATTGCTTGGTCGCTAACACTTTTAGCCATGGGATTTGGTTGCAAGGAAATTGAACTTGGGATGAGAAATGCTCGTCTGAGGACAATGGAGGCATTTCTAATTATACTTTCGGCAACACAACTTCTCTACATTGCTGCTATATACGGCGTTCGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

157

Amino Acids

16.81

Weight (kDa)

9.1

Isoelectric Point (pI)

27.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 15 - 154 3.3e-61 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 131, 185, 309
AcoI YGGCCR 1 cut(s) 186
AgeI ACCGGT 1 cut(s) 22
AgsI TTSAA 1 cut(s) 368
AhlI ACTAGT 1 cut(s) 284
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AluBI AGCT 3 cut(s) 104, 236, 299
AluI AGCT 3 cut(s) 104, 236, 299
Alw26I GTCTC 2 cut(s) 293, 307
AoxI GGCC 2 cut(s) 27, 186
ApeKI GCWGC 3 cut(s) 236, 296, 449
AsiGI ACCGGT 1 cut(s) 22
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 90
AsuC2I CCSGG 1 cut(s) 293
AsuII TTCGAA 1 cut(s) 466
AvaII GGWCC 1 cut(s) 90
BbsI GAAGAC 1 cut(s) 134
BbvI GCAGC 3 cut(s) 223, 308, 436
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 2 cut(s) 293, 307
BcuI ACTAGT 1 cut(s) 284
BfaI CTAG 1 cut(s) 285
BisI GCNGC 5 cut(s) 186, 237, 297, 309, 450
BlsI GCNGC 5 cut(s) 187, 238, 298, 310, 451
Bme1390I CCNGG 1 cut(s) 293
Bme18I GGWCC 1 cut(s) 90
BmgT120I GGNCC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 293
BmsI GCATC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 370, 402
BpmI CTGGAG 2 cut(s) 243, 252
Bpu14I TTCGAA 1 cut(s) 466
BpuMI CCSGG 1 cut(s) 293
BsaJI CCNNGG 3 cut(s) 112, 240, 342
BsaWI WCCGGW 2 cut(s) 22, 191
Bse118I RCCGGY 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 444
BseDI CCNNGG 3 cut(s) 112, 240, 342
BseGI GGATG 1 cut(s) 381
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 1 cut(s) 383
BseXI GCAGC 3 cut(s) 223, 308, 436
BshFI GGCC 2 cut(s) 29, 188
BshTI ACCGGT 1 cut(s) 22
BsiSI CCGG 5 cut(s) 23, 146, 192, 252, 293
BsmAI GTCTC 2 cut(s) 293, 307
BsnI GGCC 2 cut(s) 29, 188
Bsp119I TTCGAA 1 cut(s) 466
Bsp19I CCATGG 2 cut(s) 112, 342
BspACI CCGC 3 cut(s) 131, 185, 309
BspANI GGCC 2 cut(s) 29, 188
BspCNI CTCAG 1 cut(s) 384
BspT104I TTCGAA 1 cut(s) 466
BsrDI GCAATG 1 cut(s) 444
BsrFI RCCGGY 1 cut(s) 22
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 3 cut(s) 112, 240, 342
BssT1I CCWWGG 2 cut(s) 112, 342
BstBI TTCGAA 1 cut(s) 466
BstDEI CTNAG 1 cut(s) 392
BstDSI CCRYGG 2 cut(s) 112, 342
BstF5I GGATG 1 cut(s) 381
BstHHI GCGC 1 cut(s) 86
BstMAI GTCTC 2 cut(s) 293, 307
BstMWI GCNNNNNNNGC 5 cut(s) 218, 227, 236, 305, 317
BstSCI CCNGG 1 cut(s) 291
BstV1I GCAGC 3 cut(s) 223, 308, 436
BstV2I GAAGAC 1 cut(s) 134
BsuRI GGCC 2 cut(s) 29, 188
BtgI CCRYGG 2 cut(s) 112, 342
BtsCI GGATG 1 cut(s) 381
CfoI GCGC 1 cut(s) 86
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 1 cut(s) 90
CspAI ACCGGT 1 cut(s) 22
CviAII CATG 5 cut(s) 87, 94, 113, 273, 343
CviJI RGCY 7 cut(s) 29, 104, 149, 188, 236, 299, 341
CviKI_1 RGCY 7 cut(s) 29, 104, 149, 188, 236, 299, 341
DdeI CTNAG 1 cut(s) 392
EaeI YGGCCR 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 112, 342
Eco47I GGWCC 1 cut(s) 90
EcoT14I CCWWGG 2 cut(s) 112, 342
ErhI CCWWGG 2 cut(s) 112, 342
FaeI CATG 5 cut(s) 90, 97, 116, 276, 346
FatI CATG 5 cut(s) 86, 93, 112, 272, 342
Fnu4HI GCNGC 5 cut(s) 186, 237, 297, 309, 450
FokI GGATG 1 cut(s) 388
Fsp4HI GCNGC 5 cut(s) 186, 237, 297, 309, 450
FspBI CTAG 1 cut(s) 285
GlaI GCGC 1 cut(s) 85
GluI GCNGC 5 cut(s) 186, 237, 297, 309, 450
GsuI CTGGAG 2 cut(s) 243, 252
HaeIII GGCC 2 cut(s) 29, 188
HapII CCGG 5 cut(s) 23, 146, 192, 252, 293
HhaI GCGC 1 cut(s) 86
Hin1II CATG 5 cut(s) 90, 97, 116, 276, 346
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HinfI GANTC 1 cut(s) 195
HpaII CCGG 5 cut(s) 23, 146, 192, 252, 293
Hpy188I TCNGA 2 cut(s) 15, 393
Hpy188III TCNNGA 1 cut(s) 46
HpyCH4V TGCA 1 cut(s) 357
HpyF10VI GCNNNNNNNGC 5 cut(s) 218, 227, 236, 305, 317
HpyF3I CTNAG 1 cut(s) 392
Hsp92II CATG 5 cut(s) 90, 97, 116, 276, 346
HspAI GCGC 1 cut(s) 84
LmnI GCTCC 1 cut(s) 233
LpnPI CCDG 8 cut(s) 36, 59, 159, 205, 207, 216, 265, 306
Lsp1109I GCAGC 3 cut(s) 223, 308, 436
LweI GCATC 1 cut(s) 264
MaeI CTAG 1 cut(s) 285
MboII GAAGA 2 cut(s) 134, 190
MluCI AATT 4 cut(s) 78, 216, 363, 414
MnlI CCTC 4 cut(s) 40, 250, 387, 397
MseI TTAA 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 5 cut(s) 23, 146, 192, 252, 293
MspR9I CCNGG 1 cut(s) 293
MwoI GCNNNNNNNGC 5 cut(s) 218, 227, 236, 305, 317
NciI CCSGG 1 cut(s) 293
NcoI CCATGG 2 cut(s) 112, 342
NlaIII CATG 5 cut(s) 90, 97, 116, 276, 346
NspV TTCGAA 1 cut(s) 466
PfeI GAWTC 1 cut(s) 195
PinAI ACCGGT 1 cut(s) 22
PkrI GCNGC 5 cut(s) 187, 238, 298, 310, 451
PspPI GGNCC 1 cut(s) 90
PvuII CAGCTG 1 cut(s) 299
SaqAI TTAA 1 cut(s) 215
SatI GCNGC 5 cut(s) 186, 237, 297, 309, 450
Sau96I GGNCC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 293
SetI ASST 3 cut(s) 106, 238, 301
SfaNI GCATC 1 cut(s) 264
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 1 cut(s) 90
SpeI ACTAGT 1 cut(s) 284
Sse9I AATT 4 cut(s) 78, 216, 363, 414
SsiI CCGC 3 cut(s) 131, 185, 309
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 291
StyI CCWWGG 2 cut(s) 112, 342
TaqI TCGA 1 cut(s) 466
TasI AATT 4 cut(s) 78, 216, 363, 414
TauI GCSGC 2 cut(s) 188, 311
TfiI GAWTC 1 cut(s) 195
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TseI GCWGC 3 cut(s) 236, 296, 449
TspDTI ATGAA 3 cut(s) 32, 110, 190
VpaK11BI GGWCC 1 cut(s) 90
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.