MD10G1267900.v1.1

AWPM-19-like membrane family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009983
Physical Location & Seq
Forward (+)
36085439 .. 36086522
1084 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1267900.v1.1.491

Sequence Viewer

Length: 480 bp
ATGGCCTACCAGCAGTTAAAACCCGTTGCCGGACTGCTTTTGGCTCTCAATTTCTGCATGTATGTCATCGTTTTGGGCATTGGAGGCTGGGCAATGAACCGAGCAATCGACCACGGTTTTGTTATTGGTCCTAGTTTTGATCTCCCGGCACATTTTTCGCCAATATATTTCCCCATGGGGAACGCTGCTACAGGGTTCTTTGTGACATTCGCTCTGCTCGCTGGAGTTGTTGGCGTAGCTTCAGCTATTTCCGGTATCAACCATCTCCGATCTTGGACCTATGAGAGCTTGCCGTCTGCAGCCACAGCTGCCACCATTGCCTGGACTCTCACCGTACTTGCCATGGGCTTCGGTTGCAAGGAGATCGAGCTTCGGATCAGAAATTCCCGATTGAGAACAATGGAGGCCTTCTTGATCATCCTTTCGGCGACGCAGCTTCTGTATTTAGCAGTCATTCATACCGTTGTATCGAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

160

Amino Acids

17.24

Weight (kDa)

9.44

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 16 - 154 2.8e-65 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 321
AclWI GGATC 1 cut(s) 383
AcsI RAATTY 1 cut(s) 382
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 1 cut(s) 336
AfiI CCNNNNNNNGG 2 cut(s) 29, 321
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 6 cut(s) 239, 245, 288, 308, 370, 436
AluI AGCT 6 cut(s) 239, 245, 288, 308, 370, 436
AlwI GGATC 1 cut(s) 383
AlwNI CAGNNNCTG 1 cut(s) 439
AoxI GGCC 2 cut(s) 3, 405
ApeKI GCWGC 4 cut(s) 185, 299, 308, 433
ApoI RAATTY 1 cut(s) 382
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
AspS9I GGNCC 2 cut(s) 128, 276
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 1 cut(s) 322
AvaII GGWCC 2 cut(s) 128, 276
BaeI ACNNNNGTAYC 1 cut(s) 450
BbvI GCAGC 4 cut(s) 172, 295, 311, 445
BccI CCATC 1 cut(s) 270
BceAI ACGGC 1 cut(s) 277
BcgI CGANNNNNNTGC 4 cut(s) 138, 172, 346, 380
BciT130I CCWGG 1 cut(s) 322
BclI TGATCA 1 cut(s) 414
BcnI CCSGG 1 cut(s) 146
BfaI CTAG 1 cut(s) 132
BfmI CTRYAG 2 cut(s) 189, 297
BisI GCNGC 4 cut(s) 186, 300, 309, 434
BlsI GCNGC 4 cut(s) 187, 301, 310, 435
Bme1390I CCNGG 2 cut(s) 146, 322
Bme18I GGWCC 2 cut(s) 128, 276
BmgT120I GGNCC 2 cut(s) 128, 276
BmrFI CCNGG 2 cut(s) 146, 322
BpmI CTGGAG 1 cut(s) 243
BpuMI CCSGG 1 cut(s) 146
BsaJI CCNNGG 3 cut(s) 112, 174, 342
BsaWI WCCGGW 1 cut(s) 251
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 321
Bse3DI GCAATG 2 cut(s) 99, 315
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 3 cut(s) 112, 174, 342
BseGI GGATG 1 cut(s) 417
BseLI CCNNNNNNNGG 2 cut(s) 29, 321
BseMI GCAATG 2 cut(s) 99, 315
BseXI GCAGC 4 cut(s) 172, 295, 311, 445
BseYI CCCAGC 1 cut(s) 87
BshFI GGCC 2 cut(s) 5, 407
BsiSI CCGG 3 cut(s) 30, 146, 252
BslI CCNNNNNNNGG 2 cut(s) 29, 321
BsnI GGCC 2 cut(s) 5, 407
Bsp143I GATC 5 cut(s) 139, 269, 363, 375, 414
Bsp19I CCATGG 2 cut(s) 174, 342
BspANI GGCC 2 cut(s) 5, 407
BspMAI CTGCAG 1 cut(s) 301
BspPI GGATC 1 cut(s) 383
BsrDI GCAATG 2 cut(s) 99, 315
BssECI CCNNGG 3 cut(s) 112, 174, 342
BssMI GATC 5 cut(s) 139, 269, 363, 375, 414
BssT1I CCWWGG 2 cut(s) 174, 342
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 3 cut(s) 116, 334, 463
BstC8I GCNNGC 2 cut(s) 219, 290
BstDSI CCRYGG 3 cut(s) 112, 174, 342
BstF5I GGATG 1 cut(s) 417
BstKTI GATC 5 cut(s) 142, 272, 366, 378, 417
BstMBI GATC 5 cut(s) 139, 269, 363, 375, 414
BstMWI GCNNNNNNNGC 6 cut(s) 84, 218, 305, 308, 317, 354
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 1 cut(s) 61
BstSCI CCNGG 2 cut(s) 144, 320
BstSFI CTRYAG 2 cut(s) 189, 297
BstV1I GCAGC 4 cut(s) 172, 295, 311, 445
BsuRI GGCC 2 cut(s) 5, 407
BtgI CCRYGG 3 cut(s) 112, 174, 342
BtsCI GGATG 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 219, 290
CaiI CAGNNNCTG 1 cut(s) 439
Cfr13I GGNCC 2 cut(s) 128, 276
CseI GACGC 1 cut(s) 439
Csp6I GTAC 1 cut(s) 335
CviAII CATG 3 cut(s) 58, 175, 343
CviQI GTAC 1 cut(s) 335
DpnI GATC 5 cut(s) 141, 271, 365, 377, 416
DpnII GATC 5 cut(s) 139, 269, 363, 375, 414
Eco130I CCWWGG 2 cut(s) 174, 342
Eco147I AGGCCT 1 cut(s) 407
Eco47I GGWCC 2 cut(s) 128, 276
Eco57I CTGAAG 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 320
EcoT14I CCWWGG 2 cut(s) 174, 342
ErhI CCWWGG 2 cut(s) 174, 342
FaeI CATG 3 cut(s) 61, 178, 346
FaiI YATR 7 cut(s) 59, 63, 166, 176, 282, 344, 459
FatI CATG 3 cut(s) 57, 174, 342
FbaI TGATCA 1 cut(s) 414
Fnu4HI GCNGC 4 cut(s) 186, 300, 309, 434
FokI GGATG 1 cut(s) 404
Fsp4HI GCNGC 4 cut(s) 186, 300, 309, 434
FspBI CTAG 1 cut(s) 132
GluI GCNGC 4 cut(s) 186, 300, 309, 434
GsaI CCCAGC 1 cut(s) 91
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 2 cut(s) 5, 407
HapII CCGG 3 cut(s) 30, 146, 252
HgaI GACGC 1 cut(s) 439
Hin1II CATG 3 cut(s) 61, 178, 346
HinfI GANTC 1 cut(s) 325
HpaII CCGG 3 cut(s) 30, 146, 252
HphI GGTGA 1 cut(s) 322
Hpy188I TCNGA 3 cut(s) 269, 375, 380
Hpy188III TCNNGA 2 cut(s) 387, 412
Hpy99I CGWCG 1 cut(s) 433
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 3 cut(s) 116, 334, 463
HpyCH4V TGCA 3 cut(s) 57, 299, 357
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 218, 305, 308, 317, 354
Hsp92II CATG 3 cut(s) 61, 178, 346
Ksp22I TGATCA 1 cut(s) 414
Kzo9I GATC 5 cut(s) 139, 269, 363, 375, 414
LpnPI CCDG 9 cut(s) 23, 43, 73, 159, 177, 207, 265, 307, 334
Lsp1109I GCAGC 4 cut(s) 172, 295, 311, 445
MaeI CTAG 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 202
MalI GATC 5 cut(s) 141, 271, 365, 377, 416
MboI GATC 5 cut(s) 139, 269, 363, 375, 414
MluCI AATT 2 cut(s) 49, 382
MlyI GAGTC 1 cut(s) 319
MnlI CCTC 3 cut(s) 77, 397, 465
MseI TTAA 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 3 cut(s) 30, 146, 252
MspR9I CCNGG 2 cut(s) 146, 322
MvaI CCWGG 1 cut(s) 322
MwoI GCNNNNNNNGC 6 cut(s) 84, 218, 305, 308, 317, 354
NciI CCSGG 1 cut(s) 146
NcoI CCATGG 2 cut(s) 174, 342
NdeII GATC 5 cut(s) 139, 269, 363, 375, 414
NlaIII CATG 3 cut(s) 61, 178, 346
NmuCI GTSAC 1 cut(s) 202
NspI RCATGY 1 cut(s) 61
PceI AGGCCT 1 cut(s) 407
PflMI CCANNNNNTGG 1 cut(s) 321
PkrI GCNGC 4 cut(s) 187, 301, 310, 435
PleI GAGTC 1 cut(s) 319
PpsI GAGTC 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 320
PspFI CCCAGC 1 cut(s) 87
PspGI CCWGG 1 cut(s) 320
PspPI GGNCC 2 cut(s) 128, 276
PstI CTGCAG 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 439
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 4 cut(s) 186, 300, 309, 434
Sau3AI GATC 5 cut(s) 139, 269, 363, 375, 414
Sau96I GGNCC 2 cut(s) 128, 276
SchI GAGTC 1 cut(s) 319
ScrFI CCNGG 2 cut(s) 146, 322
SetI ASST 7 cut(s) 241, 247, 281, 290, 310, 372, 438
SfcI CTRYAG 2 cut(s) 189, 297
SinI GGWCC 2 cut(s) 128, 276
Sse9I AATT 2 cut(s) 49, 382
SseBI AGGCCT 1 cut(s) 407
SspMI CTAG 1 cut(s) 132
StuI AGGCCT 1 cut(s) 407
StyD4I CCNGG 2 cut(s) 144, 320
StyI CCWWGG 2 cut(s) 174, 342
TaaI ACNGT 3 cut(s) 116, 334, 463
TaqI TCGA 3 cut(s) 108, 366, 470
TasI AATT 2 cut(s) 49, 382
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 4 cut(s) 185, 299, 308, 433
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 2 cut(s) 110, 446
Van91I CCANNNNNTGG 1 cut(s) 321
VpaK11BI GGWCC 2 cut(s) 128, 276
XapI RAATTY 1 cut(s) 382
XceI RCATGY 1 cut(s) 61
XspI CTAG 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.