MD05G1292100.v1.1

AWPM-19-like membrane family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009983
Physical Location & Seq
Forward (+)
42355565 .. 42356487
923 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1292100.v1.1.491

Sequence Viewer

Length: 480 bp
ATGGCCAACCAGCAGTTAAAACCCGTTGCCGGTCTGCTTTTGGTTCTCAATTTATGCATGTATGTCATCGTTTTGGGCATTGGAAGCTGGGCCATCAACAGAGCAATCGACCACGGTTTCATTATTGGTCCTGATATTCATCTCCCGGCACATTTTTCCCCCATATACTTCCCCATGGGAAATGCTGCGACCGGGTTCTTTGTGACATTCTCGCTGCTCGCCGGAGTTGTTGGCGCAGCTTCAGCTATTTCCGGTATCAACCATCTTCGATCTTGGACCTCTGAGAGCTTGCCGTCTGCAGCCACAGCTGCTACCATTGCCTGGACCCTCACCGTACTTGCCATGGGCTTCGGTTGCAAGGAGATCGAGCTTCGGATCAGAAATTCCCGCTTGAGAACAATGGAGGCCTTCTTGATCATCCTTACCGCGACGCAGCTTCTGTATTTAGCAGTCATTCATACCGTTGCATCGAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

160

Amino Acids

17.12

Weight (kDa)

9.58

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 15 - 154 9.5e-65 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 321
AccII CGCG 1 cut(s) 428
AciI CCGC 2 cut(s) 388, 426
AclWI GGATC 1 cut(s) 383
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 382
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 1 cut(s) 336
AfiI CCNNNNNNNGG 2 cut(s) 29, 321
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 7 cut(s) 87, 239, 245, 288, 308, 370, 436
AluI AGCT 7 cut(s) 87, 239, 245, 288, 308, 370, 436
AlwI GGATC 1 cut(s) 383
AlwNI CAGNNNCTG 1 cut(s) 439
AoxI GGCC 3 cut(s) 3, 90, 405
ApeKI GCWGC 6 cut(s) 185, 214, 236, 299, 308, 433
ApoI RAATTY 1 cut(s) 382
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 4 cut(s) 90, 128, 276, 324
AsuC2I CCSGG 2 cut(s) 146, 193
AsuHPI GGTGA 1 cut(s) 322
AvaII GGWCC 3 cut(s) 128, 276, 324
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 6 cut(s) 172, 201, 248, 295, 311, 445
BccI CCATC 2 cut(s) 101, 270
BceAI ACGGC 1 cut(s) 277
BcgI CGANNNNNNTGC 2 cut(s) 346, 380
BciT130I CCWGG 1 cut(s) 322
BclI TGATCA 1 cut(s) 414
BcnI CCSGG 2 cut(s) 146, 193
BfmI CTRYAG 1 cut(s) 297
BisI GCNGC 6 cut(s) 186, 215, 237, 300, 309, 434
BlsI GCNGC 6 cut(s) 187, 216, 238, 301, 310, 435
Bme1390I CCNGG 3 cut(s) 146, 193, 322
Bme18I GGWCC 3 cut(s) 128, 276, 324
BmgT120I GGNCC 4 cut(s) 90, 128, 276, 324
BmiI GGNNCC 1 cut(s) 326
BmrFI CCNGG 3 cut(s) 146, 193, 322
BmsI GCATC 1 cut(s) 476
BpuEI CTTGAG 1 cut(s) 412
BpuMI CCSGG 2 cut(s) 146, 193
BsaBI GATNNNNATC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 112, 174, 342
BsaWI WCCGGW 1 cut(s) 251
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 321
Bse118I RCCGGY 1 cut(s) 29
Bse3DI GCAATG 1 cut(s) 315
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 3 cut(s) 112, 174, 342
BseGI GGATG 1 cut(s) 417
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 2 cut(s) 29, 321
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 1 cut(s) 273
BseXI GCAGC 6 cut(s) 172, 201, 248, 295, 311, 445
BseYI CCCAGC 1 cut(s) 87
Bsh1236I CGCG 1 cut(s) 428
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 3 cut(s) 5, 92, 407
BsiEI CGRYCG 1 cut(s) 192
BsiSI CCGG 5 cut(s) 30, 146, 192, 222, 252
BslI CCNNNNNNNGG 2 cut(s) 29, 321
BsnI GGCC 3 cut(s) 5, 92, 407
Bsp143I GATC 4 cut(s) 269, 363, 375, 414
Bsp19I CCATGG 2 cut(s) 174, 342
BspACI CCGC 2 cut(s) 388, 426
BspANI GGCC 3 cut(s) 5, 92, 407
BspCNI CTCAG 1 cut(s) 274
BspFNI CGCG 1 cut(s) 428
BspLI GGNNCC 1 cut(s) 326
BspMAI CTGCAG 1 cut(s) 301
BspPI GGATC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 315
BsrFI RCCGGY 1 cut(s) 29
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 3 cut(s) 112, 174, 342
BssMI GATC 4 cut(s) 269, 363, 375, 414
BssT1I CCWWGG 2 cut(s) 174, 342
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 3 cut(s) 116, 334, 463
BstC8I GCNNGC 2 cut(s) 219, 290
BstDEI CTNAG 1 cut(s) 282
BstDSI CCRYGG 3 cut(s) 112, 174, 342
BstF5I GGATG 1 cut(s) 417
BstFNI CGCG 1 cut(s) 428
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 4 cut(s) 272, 366, 378, 417
BstMBI GATC 4 cut(s) 269, 363, 375, 414
BstMCI CGRYCG 1 cut(s) 192
BstMWI GCNNNNNNNGC 6 cut(s) 84, 242, 305, 308, 317, 354
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 1 cut(s) 61
BstSCI CCNGG 3 cut(s) 144, 191, 320
BstSFI CTRYAG 1 cut(s) 297
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 6 cut(s) 172, 201, 248, 295, 311, 445
BsuRI GGCC 3 cut(s) 5, 92, 407
BtgI CCRYGG 3 cut(s) 112, 174, 342
BtsCI GGATG 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 219, 290
CaiI CAGNNNCTG 1 cut(s) 439
CfoI GCGC 1 cut(s) 236
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 4 cut(s) 90, 128, 276, 324
CseI GACGC 1 cut(s) 439
Csp6I GTAC 1 cut(s) 335
CviAII CATG 3 cut(s) 58, 175, 343
CviQI GTAC 1 cut(s) 335
DdeI CTNAG 1 cut(s) 282
DpnI GATC 4 cut(s) 271, 365, 377, 416
DpnII GATC 4 cut(s) 269, 363, 375, 414
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 2 cut(s) 174, 342
Eco147I AGGCCT 1 cut(s) 407
Eco47I GGWCC 3 cut(s) 128, 276, 324
Eco57I CTGAAG 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 320
EcoT14I CCWWGG 2 cut(s) 174, 342
EcoT22I ATGCAT 1 cut(s) 59
ErhI CCWWGG 2 cut(s) 174, 342
FaeI CATG 3 cut(s) 61, 178, 346
FaiI YATR 8 cut(s) 55, 59, 63, 164, 166, 176, 344, 459
FatI CATG 3 cut(s) 57, 174, 342
FauI CCCGC 1 cut(s) 395
FbaI TGATCA 1 cut(s) 414
Fnu4HI GCNGC 6 cut(s) 186, 215, 237, 300, 309, 434
FokI GGATG 1 cut(s) 404
Fsp4HI GCNGC 6 cut(s) 186, 215, 237, 300, 309, 434
GlaI GCGC 1 cut(s) 235
GluI GCNGC 6 cut(s) 186, 215, 237, 300, 309, 434
GsaI CCCAGC 1 cut(s) 91
HaeIII GGCC 3 cut(s) 5, 92, 407
HapII CCGG 5 cut(s) 30, 146, 192, 222, 252
HgaI GACGC 1 cut(s) 439
HhaI GCGC 1 cut(s) 236
Hin1II CATG 3 cut(s) 61, 178, 346
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HpaII CCGG 5 cut(s) 30, 146, 192, 222, 252
HphI GGTGA 1 cut(s) 322
Hpy188I TCNGA 3 cut(s) 283, 375, 380
Hpy188III TCNNGA 2 cut(s) 131, 412
Hpy99I CGWCG 1 cut(s) 433
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 3 cut(s) 116, 334, 463
HpyCH4V TGCA 4 cut(s) 57, 299, 357, 467
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 242, 305, 308, 317, 354
HpyF3I CTNAG 1 cut(s) 282
Hsp92II CATG 3 cut(s) 61, 178, 346
HspAI GCGC 1 cut(s) 234
Ksp22I TGATCA 1 cut(s) 414
Kzo9I GATC 4 cut(s) 269, 363, 375, 414
Lsp1109I GCAGC 6 cut(s) 172, 201, 248, 295, 311, 445
LweI GCATC 1 cut(s) 476
MaeIII GTNAC 1 cut(s) 202
MalI GATC 4 cut(s) 271, 365, 377, 416
MboI GATC 4 cut(s) 269, 363, 375, 414
MboII GAAGA 1 cut(s) 257
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 49, 382
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 4 cut(s) 289, 338, 397, 465
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 59
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 5 cut(s) 30, 146, 192, 222, 252
MspR9I CCNGG 3 cut(s) 146, 193, 322
MvaI CCWGG 1 cut(s) 322
MvnI CGCG 1 cut(s) 428
MwoI GCNNNNNNNGC 6 cut(s) 84, 242, 305, 308, 317, 354
NciI CCSGG 2 cut(s) 146, 193
NcoI CCATGG 2 cut(s) 174, 342
NdeII GATC 4 cut(s) 269, 363, 375, 414
NlaIII CATG 3 cut(s) 61, 178, 346
NlaIV GGNNCC 1 cut(s) 326
NmuCI GTSAC 1 cut(s) 202
NsiI ATGCAT 1 cut(s) 59
NspI RCATGY 1 cut(s) 61
PceI AGGCCT 1 cut(s) 407
PflMI CCANNNNNTGG 1 cut(s) 321
PkrI GCNGC 6 cut(s) 187, 216, 238, 301, 310, 435
Psp6I CCWGG 1 cut(s) 320
PspFI CCCAGC 1 cut(s) 87
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 1 cut(s) 326
PspPI GGNCC 4 cut(s) 90, 128, 276, 324
PstI CTGCAG 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 439
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 6 cut(s) 186, 215, 237, 300, 309, 434
Sau3AI GATC 4 cut(s) 269, 363, 375, 414
Sau96I GGNCC 4 cut(s) 90, 128, 276, 324
ScrFI CCNGG 3 cut(s) 146, 193, 322
SetI ASST 8 cut(s) 89, 241, 247, 281, 290, 310, 372, 438
SfaNI GCATC 1 cut(s) 476
SfcI CTRYAG 1 cut(s) 297
SinI GGWCC 3 cut(s) 128, 276, 324
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
Sse9I AATT 2 cut(s) 49, 382
SseBI AGGCCT 1 cut(s) 407
SsiI CCGC 2 cut(s) 388, 426
StuI AGGCCT 1 cut(s) 407
StyD4I CCNGG 3 cut(s) 144, 191, 320
StyI CCWWGG 2 cut(s) 174, 342
TaaI ACNGT 3 cut(s) 116, 334, 463
TaqI TCGA 4 cut(s) 108, 268, 366, 470
TasI AATT 2 cut(s) 49, 382
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 6 cut(s) 185, 214, 236, 299, 308, 433
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 3 cut(s) 109, 128, 446
Van91I CCANNNNNTGG 1 cut(s) 321
VpaK11BI GGWCC 3 cut(s) 128, 276, 324
XapI RAATTY 1 cut(s) 382
XceI RCATGY 1 cut(s) 61
Zsp2I ATGCAT 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.