Rroxscaffold_1G00063730

AWPM-19-like membrane family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0009983
Physical Location & Seq
Forward (+)
85513000 .. 85514075
1076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00063730.1

Sequence Viewer

Length: 477 bp
ATGGCCAGTCAGCAGTTGAAATCTGTCGCTGGTTTGCTTTTGGTTCTGAACTTCTGCATGTATGTCATTGTGCTAGGCATCGGCGGCTGGGCCATGAATAGAGCAATCGACCATGGTTTCATTATTGGTGCTGATTTTAATCTTCCTGCGCACTTCTCACCCATATACTTCCCAATGGGGAATGCTGCCACTGGATTCTTTGTGACATTTGCTTTGATCGCCGGAGTGGTTGGTGTTGCTTCAGCCATTTCTGGACTCAACCATCTTCGTTCTTGGACATCTGATAGCTTGCCATCTGCGGCCGCAGCTGCTGCCATTGCCTGGACTCTCACTCTCCTCGCCATGGGCTTTGCTTGGAAGGAGATAGAGCTTGAAATCAGGAATGCCCGCCTGAGGACAATGGAGGCATTCTTAATCATCCTTTCAGCTACTCAGCTTCTGTATATAGCAGCCATCCATGGCATTGTATCAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

158

Amino Acids

16.9

Weight (kDa)

7.98

Isoelectric Point (pI)

29.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 15 - 154 9.8e-66 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 150
AccB7I CCANNNNNTGG 1 cut(s) 321
AciI CCGC 4 cut(s) 84, 299, 303, 388
AcoI YGGCCR 2 cut(s) 3, 300
AcuI CTGAAG 1 cut(s) 225
AfiI CCNNNNNNNGG 3 cut(s) 321, 343, 393
AgsI TTSAA 2 cut(s) 19, 374
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 5 cut(s) 288, 308, 370, 428, 436
AluI AGCT 5 cut(s) 288, 308, 370, 428, 436
AlwNI CAGNNNCTG 2 cut(s) 311, 439
AoxI GGCC 3 cut(s) 3, 90, 300
ApeKI GCWGC 5 cut(s) 185, 305, 308, 311, 449
ArsI GACNNNNNNTTYG 2 cut(s) 196, 228
AspLEI GCGC 1 cut(s) 151
AspS9I GGNCC 1 cut(s) 90
AsuHPI GGTGA 1 cut(s) 150
AxyI CCTNAGG 1 cut(s) 392
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 5 cut(s) 172, 295, 298, 317, 461
BccI CCATC 3 cut(s) 270, 301, 461
BciT130I CCWGG 1 cut(s) 322
BfaI CTAG 1 cut(s) 74
BisI GCNGC 8 cut(s) 85, 186, 300, 303, 306, 309, 312, 450
BlsI GCNGC 8 cut(s) 86, 187, 301, 304, 307, 310, 313, 451
Bme1390I CCNGG 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 322
BmsI GCATC 1 cut(s) 87
BsaBI GATNNNNATC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 112, 342, 457
Bsc4I CCNNNNNNNGG 3 cut(s) 321, 343, 393
Bse1I ACTGG 2 cut(s) 6, 196
Bse21I CCTNAGG 1 cut(s) 392
Bse3DI GCAATG 1 cut(s) 315
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 3 cut(s) 112, 342, 457
BseGI GGATG 2 cut(s) 417, 453
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 3 cut(s) 321, 343, 393
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 2 cut(s) 383, 446
BseNI ACTGG 2 cut(s) 6, 196
BseRI GAGGAG 1 cut(s) 326
BseX3I CGGCCG 1 cut(s) 300
BseXI GCAGC 5 cut(s) 172, 295, 298, 317, 461
BseYI CCCAGC 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 303
BshFI GGCC 3 cut(s) 5, 92, 302
BsiEI CGRYCG 1 cut(s) 303
BsiSI CCGG 1 cut(s) 222
BslI CCNNNNNNNGG 3 cut(s) 321, 343, 393
BsmI GAATGC 3 cut(s) 187, 388, 407
BsnI GGCC 3 cut(s) 5, 92, 302
Bsp143I GATC 1 cut(s) 216
Bsp19I CCATGG 3 cut(s) 112, 342, 457
BspACI CCGC 4 cut(s) 84, 299, 303, 388
BspANI GGCC 3 cut(s) 5, 92, 302
BspCNI CTCAG 2 cut(s) 384, 445
BsrDI GCAATG 1 cut(s) 315
BsrI ACTGG 2 cut(s) 6, 196
BssECI CCNNGG 3 cut(s) 112, 342, 457
BssMI GATC 1 cut(s) 216
BssT1I CCWWGG 3 cut(s) 112, 342, 457
Bst2UI CCWGG 1 cut(s) 322
BstAPI GCANNNNNTGC 1 cut(s) 311
BstC8I GCNNGC 2 cut(s) 290, 388
BstDEI CTNAG 2 cut(s) 392, 432
BstDSI CCRYGG 3 cut(s) 112, 342, 457
BstF5I GGATG 2 cut(s) 417, 453
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 1 cut(s) 219
BstMBI GATC 1 cut(s) 216
BstMCI CGRYCG 1 cut(s) 303
BstMWI GCNNNNNNNGC 6 cut(s) 84, 218, 305, 308, 311, 317
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 320
BstV1I GCAGC 5 cut(s) 172, 295, 298, 317, 461
BstZI CGGCCG 1 cut(s) 300
Bsu36I CCTNAGG 1 cut(s) 392
BsuRI GGCC 3 cut(s) 5, 92, 302
BtgI CCRYGG 3 cut(s) 112, 342, 457
BtsCI GGATG 2 cut(s) 417, 453
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 2 cut(s) 290, 388
CaiI CAGNNNCTG 2 cut(s) 311, 439
CciNI GCGGCCGC 1 cut(s) 300
CfoI GCGC 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 90
CviAII CATG 5 cut(s) 58, 94, 113, 343, 458
DdeI CTNAG 2 cut(s) 392, 432
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
EaeI YGGCCR 2 cut(s) 3, 300
EagI CGGCCG 1 cut(s) 300
EclXI CGGCCG 1 cut(s) 300
Eco130I CCWWGG 3 cut(s) 112, 342, 457
Eco52I CGGCCG 1 cut(s) 300
Eco57I CTGAAG 1 cut(s) 225
Eco81I CCTNAGG 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 320
EcoT14I CCWWGG 3 cut(s) 112, 342, 457
ErhI CCWWGG 3 cut(s) 112, 342, 457
FaeI CATG 5 cut(s) 61, 97, 116, 346, 461
FatI CATG 5 cut(s) 57, 93, 112, 342, 457
FauI CCCGC 1 cut(s) 395
Fnu4HI GCNGC 8 cut(s) 85, 186, 300, 303, 306, 309, 312, 450
FokI GGATG 2 cut(s) 404, 440
Fsp4HI GCNGC 8 cut(s) 85, 186, 300, 303, 306, 309, 312, 450
FspBI CTAG 1 cut(s) 74
FspI TGCGCA 1 cut(s) 150
GlaI GCGC 1 cut(s) 150
GluI GCNGC 8 cut(s) 85, 186, 300, 303, 306, 309, 312, 450
GsaI CCCAGC 1 cut(s) 91
HaeIII GGCC 3 cut(s) 5, 92, 302
HapII CCGG 1 cut(s) 222
HhaI GCGC 1 cut(s) 151
Hin1II CATG 5 cut(s) 61, 97, 116, 346, 461
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HinfI GANTC 3 cut(s) 195, 255, 325
HpaII CCGG 1 cut(s) 222
HphI GGTGA 1 cut(s) 150
Hpy188I TCNGA 2 cut(s) 48, 283
Hpy188III TCNNGA 3 cut(s) 252, 379, 471
HpyAV CCTTC 1 cut(s) 352
HpyCH4V TGCA 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 218, 305, 308, 311, 317
HpyF3I CTNAG 2 cut(s) 392, 432
Hsp92II CATG 5 cut(s) 61, 97, 116, 346, 461
HspAI GCGC 1 cut(s) 149
Kzo9I GATC 1 cut(s) 216
Lsp1109I GCAGC 5 cut(s) 172, 295, 298, 317, 461
LweI GCATC 1 cut(s) 87
MaeI CTAG 1 cut(s) 74
MaeIII GTNAC 1 cut(s) 202
MalI GATC 1 cut(s) 218
MboI GATC 1 cut(s) 216
MboII GAAGA 2 cut(s) 134, 257
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 249, 319
MnlI CCTC 3 cut(s) 347, 387, 397
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 138, 413
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 1 cut(s) 322
Mva1269I GAATGC 3 cut(s) 187, 388, 407
MvaI CCWGG 1 cut(s) 322
MwoI GCNNNNNNNGC 6 cut(s) 84, 218, 305, 308, 311, 317
NcoI CCATGG 3 cut(s) 112, 342, 457
NdeII GATC 1 cut(s) 216
NlaIII CATG 5 cut(s) 61, 97, 116, 346, 461
NmuCI GTSAC 1 cut(s) 202
NotI GCGGCCGC 1 cut(s) 300
NsbI TGCGCA 1 cut(s) 150
NspI RCATGY 1 cut(s) 61
PctI GAATGC 3 cut(s) 187, 388, 407
PfeI GAWTC 1 cut(s) 195
PflMI CCANNNNNTGG 1 cut(s) 321
PkrI GCNGC 8 cut(s) 86, 187, 301, 304, 307, 310, 313, 451
PleI GAGTC 2 cut(s) 249, 319
PpsI GAGTC 2 cut(s) 249, 319
Psp6I CCWGG 1 cut(s) 320
PspFI CCCAGC 1 cut(s) 87
PspGI CCWGG 1 cut(s) 320
PspPI GGNCC 1 cut(s) 90
PstNI CAGNNNCTG 2 cut(s) 311, 439
PvuII CAGCTG 1 cut(s) 308
SaqAI TTAA 2 cut(s) 138, 413
SatI GCNGC 8 cut(s) 85, 186, 300, 303, 306, 309, 312, 450
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 1 cut(s) 90
SchI GAGTC 2 cut(s) 249, 319
ScrFI CCNGG 1 cut(s) 322
SetI ASST 5 cut(s) 290, 310, 372, 430, 438
SfaNI GCATC 1 cut(s) 87
SsiI CCGC 4 cut(s) 84, 299, 303, 388
SspMI CTAG 1 cut(s) 74
StyD4I CCNGG 1 cut(s) 320
StyI CCWWGG 3 cut(s) 112, 342, 457
TaqI TCGA 1 cut(s) 108
TauI GCSGC 3 cut(s) 87, 302, 305
TfiI GAWTC 1 cut(s) 195
Tru1I TTAA 2 cut(s) 138, 413
Tru9I TTAA 2 cut(s) 138, 413
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 5 cut(s) 185, 305, 308, 311, 449
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 2 cut(s) 109, 110
TspRI CASTG 1 cut(s) 196
Van91I CCANNNNNTGG 1 cut(s) 321
XceI RCATGY 1 cut(s) 61
XspI CTAG 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.