Rorug05G0004900

AWPM-19-like membrane family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009983
Physical Location & Seq
Forward (+)
308674 .. 310974
2301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0004900.1

Sequence Viewer

Length: 666 bp
ATGAGCTCATACTTTTGCAGCAACCACAAGCTTCTGAGGAATAGCATCAACATGTACAACTATAATATCTTCTTCTTCTTCTTCTTCTGCTGTCTCATCACAGTTTCAAGGGCGGATGATCATGATCAAGTAGCAGTGCAGCAGCAAAACCCGTTACACCAGTTACTCTCCAGCCGAAAAGATATGGTGCAGATTGCTGGATATGGAGAAGAGAAGCTTTCTACAGTACTAATTACTGGCTCCGTCCATTGTGAAGAGGCTTGTAGTCTGTCCGATGATCATGAACATCAATCTCATCAGCTTCATCTTCATGCATGGCCTGTGTCAGGTGCTGTGGTGTCTGTCAATTGCCGTGTGGGTGGGAGAAAAAGGAAATTAAGTCGGTCACAAGGTGTCACAGATGAATATGGAGATTTCATGATTGATCTACCTTCCGATCTACACGCAGTTCCCAACTTGGACAAACTATGTTGCGCAAGAGTTATTCGGGTACCAAAGAACACACAGTGCAAAGCAGCATTTGTAAAGAGGCACAAGGGACTGCAATTATCATCAGTTGGTAATGGTATCAGAACTTACAGTGCTGGAAGGATTAGATTCCAGAATTTGACACCTAAGCCTCCACAAGCATGCATTAAGAAAGCAAGCACCAACAAACAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

221

Amino Acids

24.87

Weight (kDa)

9.21

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 78 - 173 2.7e-08 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 475
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 490
AciI CCGC 1 cut(s) 113
AcsI RAATTY 1 cut(s) 604
AdeI CACNNNGTG 2 cut(s) 392, 507
AfaI GTAC 3 cut(s) 56, 228, 492
AfiI CCNNNNNNNGG 1 cut(s) 326
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 1 cut(s) 108
AluBI AGCT 4 cut(s) 6, 31, 217, 301
AluI AGCT 4 cut(s) 6, 31, 217, 301
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 98
AlwNI CAGNNNCTG 1 cut(s) 332
AoxI GGCC 1 cut(s) 317
ApeKI GCWGC 4 cut(s) 18, 139, 142, 515
ApoI RAATTY 1 cut(s) 604
Asp718I GGTACC 1 cut(s) 490
AspLEI GCGC 1 cut(s) 476
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 4 cut(s) 30, 151, 154, 527
BceAI ACGGC 1 cut(s) 336
BclI TGATCA 3 cut(s) 118, 124, 277
BcoDI GTCTC 1 cut(s) 98
BfmI CTRYAG 1 cut(s) 222
BisI GCNGC 4 cut(s) 19, 140, 143, 516
BlsI GCNGC 4 cut(s) 20, 141, 144, 517
BmcAI AGTACT 1 cut(s) 228
BmiI GGNNCC 2 cut(s) 241, 492
BmsI GCATC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 154
Bpu10I CCTNAGC 1 cut(s) 615
BsaBI GATNNNNATC 1 cut(s) 123
Bsc4I CCNNNNNNNGG 1 cut(s) 326
Bse1I ACTGG 2 cut(s) 160, 241
Bse8I GATNNNNATC 1 cut(s) 123
BseGI GGATG 1 cut(s) 121
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 2 cut(s) 160, 241
BseXI GCAGC 4 cut(s) 30, 151, 154, 527
BsgI GTGCAG 2 cut(s) 158, 209
BshFI GGCC 1 cut(s) 319
BshNI GGYRCC 1 cut(s) 490
BsiHKAI GWGCWC 1 cut(s) 8
BslFI GGGAC 1 cut(s) 552
BslI CCNNNNNNNGG 1 cut(s) 326
BsmAI GTCTC 1 cut(s) 98
BsmFI GGGAC 1 cut(s) 552
BsnI GGCC 1 cut(s) 319
Bsp1286I GDGCHC 1 cut(s) 8
Bsp1407I TGTACA 1 cut(s) 54
Bsp143I GATC 5 cut(s) 118, 124, 277, 424, 436
BspACI CCGC 1 cut(s) 113
BspANI GGCC 1 cut(s) 319
BspCNI CTCAG 1 cut(s) 27
BspHI TCATGA 3 cut(s) 121, 280, 417
BspLI GGNNCC 2 cut(s) 241, 492
BspT107I GGYRCC 1 cut(s) 490
BsrGI TGTACA 1 cut(s) 54
BsrI ACTGG 2 cut(s) 160, 241
BssMI GATC 5 cut(s) 118, 124, 277, 424, 436
Bst4CI ACNGT 4 cut(s) 103, 226, 507, 581
Bst6I CTCTTC 2 cut(s) 204, 249
BstAUI TGTACA 1 cut(s) 54
BstC8I GCNNGC 2 cut(s) 631, 646
BstDEI CTNAG 2 cut(s) 35, 615
BstENI CCTNNNNNAGG 1 cut(s) 324
BstF5I GGATG 1 cut(s) 121
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 5 cut(s) 121, 127, 280, 427, 439
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 5 cut(s) 118, 124, 277, 424, 436
BstNSI RCATGY 2 cut(s) 55, 633
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 4 cut(s) 30, 151, 154, 527
BsuRI GGCC 1 cut(s) 319
BtsCI GGATG 1 cut(s) 121
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 3 cut(s) 141, 512, 586
Cac8I GCNNGC 2 cut(s) 631, 646
CaiI CAGNNNCTG 1 cut(s) 332
CciI TCATGA 3 cut(s) 121, 280, 417
CfoI GCGC 1 cut(s) 476
Csp6I GTAC 3 cut(s) 55, 227, 491
CviAII CATG 7 cut(s) 52, 122, 281, 311, 315, 418, 630
CviJI RGCY 9 cut(s) 6, 31, 174, 217, 240, 260, 301, 319, 619
CviKI_1 RGCY 9 cut(s) 6, 31, 174, 217, 240, 260, 301, 319, 619
CviQI GTAC 3 cut(s) 55, 227, 491
DdeI CTNAG 2 cut(s) 35, 615
DpnI GATC 5 cut(s) 120, 126, 279, 426, 438
DpnII GATC 5 cut(s) 118, 124, 277, 424, 436
DraIII CACNNNGTG 2 cut(s) 392, 507
Eam1104I CTCTTC 2 cut(s) 204, 249
EarI CTCTTC 2 cut(s) 204, 249
EciI GGCGGA 1 cut(s) 128
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 8
Eco53kI GAGCTC 1 cut(s) 6
EcoICRI GAGCTC 1 cut(s) 6
EcoNI CCTNNNNNAGG 1 cut(s) 324
EcoT22I ATGCAT 2 cut(s) 316, 635
EcoT38I GRGCYC 1 cut(s) 8
FaeI CATG 7 cut(s) 55, 125, 284, 314, 318, 421, 633
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 1 cut(s) 552
FatI CATG 7 cut(s) 51, 121, 280, 310, 314, 417, 629
FbaI TGATCA 3 cut(s) 118, 124, 277
Fnu4HI GCNGC 4 cut(s) 19, 140, 143, 516
FokI GGATG 1 cut(s) 128
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 4 cut(s) 19, 140, 143, 516
FspI TGCGCA 1 cut(s) 475
GlaI GCGC 1 cut(s) 475
GluI GCNGC 4 cut(s) 19, 140, 143, 516
GsuI CTGGAG 1 cut(s) 154
HaeIII GGCC 1 cut(s) 319
HhaI GCGC 1 cut(s) 476
Hin1II CATG 7 cut(s) 55, 125, 284, 314, 318, 421, 633
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HindIII AAGCTT 2 cut(s) 29, 215
HinfI GANTC 1 cut(s) 597
Hpy188I TCNGA 4 cut(s) 36, 274, 436, 572
Hpy188III TCNNGA 4 cut(s) 122, 281, 418, 601
HpyAV CCTTC 2 cut(s) 441, 582
HpyCH4III ACNGT 4 cut(s) 103, 226, 507, 581
HpyCH4V TGCA 7 cut(s) 18, 139, 190, 314, 510, 544, 633
HpyF3I CTNAG 2 cut(s) 35, 615
Hsp92II CATG 7 cut(s) 55, 125, 284, 314, 318, 421, 633
HspAI GCGC 1 cut(s) 474
KpnI GGTACC 1 cut(s) 494
Ksp22I TGATCA 3 cut(s) 118, 124, 277
Kzo9I GATC 5 cut(s) 118, 124, 277, 424, 436
LmnI GCTCC 1 cut(s) 245
LpnPI CCDG 8 cut(s) 173, 183, 184, 222, 312, 333, 570, 614
Lsp1109I GCAGC 4 cut(s) 30, 151, 154, 527
LweI GCATC 1 cut(s) 54
MaeIII GTNAC 4 cut(s) 153, 162, 384, 394
MalI GATC 5 cut(s) 120, 126, 279, 426, 438
MboI GATC 5 cut(s) 118, 124, 277, 424, 436
MboII GAAGA 9 cut(s) 61, 64, 67, 70, 73, 76, 221, 266, 299
MfeI CAATTG 1 cut(s) 346
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 5 cut(s) 231, 346, 374, 545, 604
MnlI CCTC 4 cut(s) 30, 250, 522, 630
Mph1103I ATGCAT 2 cut(s) 316, 635
MseI TTAA 2 cut(s) 377, 636
MslI CAYNNNNRTG 3 cut(s) 50, 309, 628
MunI CAATTG 1 cut(s) 346
NdeII GATC 5 cut(s) 118, 124, 277, 424, 436
NlaIII CATG 7 cut(s) 55, 125, 284, 314, 318, 421, 633
NlaIV GGNNCC 2 cut(s) 241, 492
NmuCI GTSAC 2 cut(s) 384, 394
NsbI TGCGCA 1 cut(s) 475
NsiI ATGCAT 2 cut(s) 316, 635
NspI RCATGY 2 cut(s) 55, 633
PaeI GCATGC 1 cut(s) 633
PagI TCATGA 3 cut(s) 121, 280, 417
PciI ACATGT 1 cut(s) 51
PfeI GAWTC 1 cut(s) 597
PkrI GCNGC 4 cut(s) 20, 141, 144, 517
PscI ACATGT 1 cut(s) 51
Psp124BI GAGCTC 1 cut(s) 8
PspN4I GGNNCC 2 cut(s) 241, 492
PstNI CAGNNNCTG 1 cut(s) 332
RsaI GTAC 3 cut(s) 56, 228, 492
RsaNI GTAC 3 cut(s) 55, 227, 491
RseI CAYNNNNRTG 3 cut(s) 50, 309, 628
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 2 cut(s) 377, 636
SatI GCNGC 4 cut(s) 19, 140, 143, 516
Sau3AI GATC 5 cut(s) 118, 124, 277, 424, 436
ScaI AGTACT 1 cut(s) 228
SduI GDGCHC 1 cut(s) 8
SetI ASST 8 cut(s) 8, 33, 219, 303, 331, 394, 433, 616
SfaNI GCATC 1 cut(s) 54
SfcI CTRYAG 1 cut(s) 222
SmiMI CAYNNNNRTG 3 cut(s) 50, 309, 628
SphI GCATGC 1 cut(s) 633
Sse9I AATT 5 cut(s) 231, 346, 374, 545, 604
SsiI CCGC 1 cut(s) 113
SstI GAGCTC 1 cut(s) 8
TaaI ACNGT 4 cut(s) 103, 226, 507, 581
TaqII GACCGA 1 cut(s) 372
TasI AATT 5 cut(s) 231, 346, 374, 545, 604
TatI WGTACW 2 cut(s) 54, 226
TfiI GAWTC 1 cut(s) 597
Tru1I TTAA 2 cut(s) 377, 636
Tru9I TTAA 2 cut(s) 377, 636
TscAI CASTG 3 cut(s) 141, 512, 586
TseFI GTSAC 2 cut(s) 384, 394
TseI GCWGC 4 cut(s) 18, 139, 142, 515
Tsp45I GTSAC 2 cut(s) 384, 394
TspDTI ATGAA 5 cut(s) 293, 297, 299, 406, 417
TspGWI ACGGA 1 cut(s) 232
TspRI CASTG 3 cut(s) 141, 512, 586
XagI CCTNNNNNAGG 1 cut(s) 324
XapI RAATTY 1 cut(s) 604
XceI RCATGY 2 cut(s) 55, 633
ZrmI AGTACT 1 cut(s) 228
Zsp2I ATGCAT 2 cut(s) 316, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.