AT5G47470

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007902
Physical Location & Seq
Forward (+)
19254403 .. 19256810
2408 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G47470.1

Sequence Viewer

Length: 1095 bp
ATGCAATTTTTTTGTGTAAATTTGTATCGATCGGTGCTCAATCTCCTTGAGGAGAGGATGAAGACGGAGATGATAGAAGAGATGGTGATAGTAGGAGGTTTAGTAATGGTTCAATTTGTATACGCAGGCAATTCTTTGCTCATGAGTTATTTGATGTCACTCGGTCTTGGCCCTTTCACCATTGTCATCTTTTCTACATTCGCCACTTTCATAATCCTCTCCCCTTTCGCCATTCTCTTCGAAAGGAAGCAATGGCCAAATGAGCTTAGCTTGAGGCTGATTGGTAAGCTAGTTTTGATCTCCTTTGCAGGGGTTACTCTGTTCCAGTCTCTGTTTCTAGAAGGAATCAGGTTGACATCACCAGCAATGGCAACAGCGATGCCAAATCTTGCTCCTGGTCTCATTTTTTTCATCGCTTGGATCGTTGGGTTAGAGAAGATGAATCTGAAATGTGTGTACAGCAAGTTGAAGATCTTAGGGACATTGTTGTGCGTATTTGGCGCTTTAGCTATGAGCGTAATGCACAGCACTTCGATCAGTCACAAAGAAGAAGATGACACTCCAATATTCGTCTTTGATCGAGACAAGGTCGTTGGATGTATCTACCTTCTTGGAGCCGTCTTTGTTTTATCCACCAACGTCGTTCTTCAGGCGTCAACGTTGGCAGAGTTCCCGGCACCGATATCGTTAAGTGCAATAACGGCATTACTAGGAGTGTTGATAACAACGGTGGTACTACTATTACAAAACCGAAAAACAAAAGTACTTGCCAGTTCATTAATTAGTTTCGGTAACCTCGTTGGTTATTCTGTCCTGGCAGGGGCAGTGAGTGGAGCATGTGTTAGCTTTAATGGATGGGCCATGAAGAAACGTGGACCGGTTTTCGTTTCAATGTTTAGTCCTTTCGCTACCGTTATTTCGGTTGCTTTCGCCGTTTTGACATTAGGAGAGTCCGTTAGCCTTGGAAGTGTGGGAGGGATGGTGTTGATGTTCGTAGGGCTATACCTTGTGTTATGGGCTAAAGGCAAAGAAGGGTTCTCTGAAATTGAGAGTTTTGAGAGTGAGTTCGACTCTAAGAAGCCTCTTTTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

364

Amino Acids

39.56

Weight (kDa)

8.21

Isoelectric Point (pI)

37.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 35 - 172 2.1e-08 EamA-like transporter family
EamA PF00892 199 - 338 5.5e-09 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 676
AccI GTMKAC 1 cut(s) 120
AclI AACGTT 1 cut(s) 659
AclWI GGATC 1 cut(s) 428
AcoI YGGCCR 1 cut(s) 254
AcsI RAATTY 1 cut(s) 19
AcuI CTGAAG 1 cut(s) 632
AcyI GRCGYC 1 cut(s) 653
AfaI GTAC 3 cut(s) 458, 735, 765
AfiI CCNNNNNNNGG 2 cut(s) 309, 820
AgeI ACCGGT 1 cut(s) 877
AgsI TTSAA 3 cut(s) 113, 469, 891
AjnI CCWGG 2 cut(s) 394, 813
AluBI AGCT 5 cut(s) 265, 270, 289, 509, 846
AluI AGCT 5 cut(s) 265, 270, 289, 509, 846
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 3 cut(s) 333, 404, 576
AlwI GGATC 1 cut(s) 428
AlwNI CAGNNNCTG 1 cut(s) 331
AoxI GGCC 3 cut(s) 169, 254, 858
ApoI RAATTY 1 cut(s) 19
AseI ATTAAT 1 cut(s) 779
AsiGI ACCGGT 1 cut(s) 877
AspLEI GCGC 1 cut(s) 503
AspS9I GGNCC 3 cut(s) 170, 858, 875
AsuC2I CCSGG 1 cut(s) 674
AsuHPI GGTGA 3 cut(s) 97, 169, 351
AsuII TTCGAA 1 cut(s) 240
AvaII GGWCC 1 cut(s) 875
BalI TGGCCA 1 cut(s) 256
BanI GGYRCC 1 cut(s) 676
BbsI GAAGAC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 39
BccI CCATC 3 cut(s) 76, 849, 973
BceAI ACGGC 3 cut(s) 602, 717, 917
BcgI CGANNNNNNTGC 2 cut(s) 666, 700
BciT130I CCWGG 2 cut(s) 396, 815
BcnI CCSGG 1 cut(s) 674
BcoDI GTCTC 3 cut(s) 333, 404, 576
BfaI CTAG 3 cut(s) 290, 338, 710
BfoI RGCGCY 1 cut(s) 504
BglII AGATCT 1 cut(s) 471
BlpI GCTNAGC 1 cut(s) 266
BmcAI AGTACT 1 cut(s) 765
Bme1390I CCNGG 3 cut(s) 396, 674, 815
Bme18I GGWCC 1 cut(s) 875
BmgT120I GGNCC 3 cut(s) 170, 858, 875
BmiI GGNNCC 2 cut(s) 616, 678
BmrFI CCNGG 3 cut(s) 396, 674, 815
BmsI GCATC 1 cut(s) 369
BpiI GAAGAC 1 cut(s) 68
BplI GAGNNNNNCTC 2 cut(s) 1055, 1087
Bpu1102I GCTNAGC 1 cut(s) 266
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 2 cut(s) 68, 292
BpuMI CCSGG 1 cut(s) 674
Bsa29I ATCGAT 1 cut(s) 28
BsaHI GRCGYC 1 cut(s) 653
BsaI GGTCTC 1 cut(s) 404
BsaJI CCNNGG 1 cut(s) 961
BsaWI WCCGGW 1 cut(s) 877
BsaXI ACNNNNNCTCC 8 cut(s) 87, 117, 825, 855, 939, 966, 969, 996
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 820
Bse118I RCCGGY 1 cut(s) 877
Bse1I ACTGG 2 cut(s) 325, 771
Bse3DI GCAATG 2 cut(s) 257, 372
BseBI CCWGG 2 cut(s) 396, 815
BseCI ATCGAT 1 cut(s) 28
BseDI CCNNGG 1 cut(s) 961
BseGI GGATG 4 cut(s) 63, 602, 860, 984
BseLI CCNNNNNNNGG 2 cut(s) 309, 820
BseMI GCAATG 2 cut(s) 257, 372
BseNI ACTGG 2 cut(s) 325, 771
BseRI GAGGAG 1 cut(s) 65
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 3 cut(s) 171, 256, 860
BshNI GGYRCC 1 cut(s) 676
BshTI ACCGGT 1 cut(s) 877
BshVI ATCGAT 1 cut(s) 28
BsiEI CGRYCG 1 cut(s) 32
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 2 cut(s) 674, 878
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 2 cut(s) 309, 820
BsmAI GTCTC 3 cut(s) 333, 404, 576
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 3 cut(s) 171, 256, 860
Bso31I GGTCTC 1 cut(s) 404
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 39
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 6 cut(s) 29, 297, 420, 471, 534, 577
Bsp1720I GCTNAGC 1 cut(s) 266
BspANI GGCC 3 cut(s) 171, 256, 860
BspDI ATCGAT 1 cut(s) 28
BspHI TCATGA 1 cut(s) 141
BspLI GGNNCC 2 cut(s) 616, 678
BspPI GGATC 1 cut(s) 428
BspT104I TTCGAA 1 cut(s) 240
BspT107I GGYRCC 1 cut(s) 676
BspTNI GGTCTC 1 cut(s) 404
BsrDI GCAATG 2 cut(s) 257, 372
BsrFI RCCGGY 1 cut(s) 877
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 2 cut(s) 325, 771
BssAI RCCGGY 1 cut(s) 877
BssECI CCNNGG 1 cut(s) 961
BssMI GATC 6 cut(s) 29, 297, 420, 471, 534, 577
BssNAI GTATAC 1 cut(s) 121
BssNI GRCGYC 1 cut(s) 653
BssT1I CCWWGG 1 cut(s) 961
Bst1107I GTATAC 1 cut(s) 121
Bst2UI CCWGG 2 cut(s) 396, 815
Bst4CI ACNGT 2 cut(s) 730, 913
Bst6I CTCTTC 2 cut(s) 72, 242
BstACI GRCGYC 1 cut(s) 653
BstAUI TGTACA 1 cut(s) 456
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 3 cut(s) 266, 475, 1074
BstEII GGTNACC 1 cut(s) 791
BstF5I GGATG 4 cut(s) 63, 602, 860, 984
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 1 cut(s) 503
BstKTI GATC 6 cut(s) 32, 300, 423, 474, 537, 580
BstMAI GTCTC 3 cut(s) 333, 404, 576
BstMBI GATC 6 cut(s) 29, 297, 420, 471, 534, 577
BstMCI CGRYCG 1 cut(s) 32
BstMWI GCNNNNNNNGC 3 cut(s) 262, 498, 701
BstNI CCWGG 2 cut(s) 396, 815
BstNSI RCATGY 1 cut(s) 840
BstPI GGTNACC 1 cut(s) 791
BstSCI CCNGG 3 cut(s) 394, 672, 813
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 471
BstYI RGATCY 1 cut(s) 471
BstZ17I GTATAC 1 cut(s) 121
Bsu15I ATCGAT 1 cut(s) 28
BsuRI GGCC 3 cut(s) 171, 256, 860
BsuTUI ATCGAT 1 cut(s) 28
BtgZI GCGATG 2 cut(s) 392, 397
BtsCI GGATG 4 cut(s) 63, 602, 860, 984
BtsI GCAGTG 1 cut(s) 831
BtsIMutI CAGTG 1 cut(s) 831
Cac8I GCNNGC 1 cut(s) 127
CaiI CAGNNNCTG 1 cut(s) 331
CciI TCATGA 1 cut(s) 141
CfoI GCGC 1 cut(s) 503
Cfr10I RCCGGY 1 cut(s) 877
Cfr13I GGNCC 3 cut(s) 170, 858, 875
ClaI ATCGAT 1 cut(s) 28
CseI GACGC 1 cut(s) 642
Csp6I GTAC 3 cut(s) 457, 734, 764
CspAI ACCGGT 1 cut(s) 877
CviAII CATG 3 cut(s) 142, 837, 862
CviQI GTAC 3 cut(s) 457, 734, 764
DdeI CTNAG 3 cut(s) 266, 475, 1074
DpnI GATC 6 cut(s) 31, 299, 422, 473, 536, 579
DpnII GATC 6 cut(s) 29, 297, 420, 471, 534, 577
EaeI YGGCCR 1 cut(s) 254
Eam1104I CTCTTC 2 cut(s) 72, 242
EarI CTCTTC 2 cut(s) 72, 242
Eco130I CCWWGG 1 cut(s) 961
Eco31I GGTCTC 1 cut(s) 404
Eco32I GATATC 1 cut(s) 684
Eco47I GGWCC 1 cut(s) 875
Eco57I CTGAAG 1 cut(s) 632
Eco91I GGTNACC 1 cut(s) 791
EcoO65I GGTNACC 1 cut(s) 791
EcoRII CCWGG 2 cut(s) 394, 813
EcoRV GATATC 1 cut(s) 684
EcoT14I CCWWGG 1 cut(s) 961
ErhI CCWWGG 1 cut(s) 961
FaeI CATG 3 cut(s) 145, 840, 865
FaiI YATR 8 cut(s) 121, 143, 212, 512, 838, 863, 1003, 1015
FaqI GGGAC 1 cut(s) 493
FatI CATG 3 cut(s) 141, 836, 861
FblI GTMKAC 1 cut(s) 120
FokI GGATG 4 cut(s) 70, 609, 867, 991
FspBI CTAG 3 cut(s) 290, 338, 710
GlaI GCGC 1 cut(s) 502
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 3 cut(s) 171, 256, 860
HapII CCGG 2 cut(s) 674, 878
HgaI GACGC 1 cut(s) 642
HhaI GCGC 1 cut(s) 503
Hin1I GRCGYC 1 cut(s) 653
Hin1II CATG 3 cut(s) 145, 840, 865
Hin6I GCGC 1 cut(s) 501
HinP1I GCGC 1 cut(s) 501
HincII GTYRAC 2 cut(s) 354, 657
HindII GTYRAC 2 cut(s) 354, 657
HinfI GANTC 4 cut(s) 345, 442, 950, 1070
HpaII CCGG 2 cut(s) 674, 878
HphI GGTGA 3 cut(s) 97, 169, 351
Hpy166II GTNNAC 5 cut(s) 121, 354, 457, 657, 875
Hpy188I TCNGA 2 cut(s) 447, 1042
Hpy188III TCNNGA 4 cut(s) 142, 338, 581, 1092
Hpy8I GTNNAC 5 cut(s) 121, 354, 457, 657, 875
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 3 cut(s) 335, 617, 1025
HpyCH4III ACNGT 2 cut(s) 730, 913
HpyCH4IV ACGT 3 cut(s) 639, 659, 871
HpyCH4V TGCA 4 cut(s) 4, 308, 523, 695
HpyF10VI GCNNNNNNNGC 3 cut(s) 262, 498, 701
HpyF3I CTNAG 3 cut(s) 266, 475, 1074
HpySE526I ACGT 3 cut(s) 639, 659, 871
Hsp92I GRCGYC 1 cut(s) 653
Hsp92II CATG 3 cut(s) 145, 840, 865
HspAI GCGC 1 cut(s) 501
Kzo9I GATC 6 cut(s) 29, 297, 420, 471, 534, 577
LmnI GCTCC 3 cut(s) 397, 614, 833
LweI GCATC 1 cut(s) 369
MaeI CTAG 3 cut(s) 290, 338, 710
MaeII ACGT 3 cut(s) 639, 659, 871
MaeIII GTNAC 4 cut(s) 156, 313, 539, 791
MalI GATC 6 cut(s) 31, 299, 422, 473, 536, 579
MboI GATC 6 cut(s) 29, 297, 420, 471, 534, 577
MboII GAAGA 9 cut(s) 73, 89, 229, 448, 481, 560, 563, 638, 877
MflI RGATCY 1 cut(s) 471
MhlI GDGCHC 1 cut(s) 39
MlsI TGGCCA 1 cut(s) 256
MluCI AATT 6 cut(s) 5, 19, 113, 130, 780, 1044
MluNI TGGCCA 1 cut(s) 256
MlyI GAGTC 2 cut(s) 959, 1064
MmeI TCCRAC 1 cut(s) 574
MnlI CCTC 8 cut(s) 43, 48, 89, 227, 267, 806, 968, 1092
Mox20I TGGCCA 1 cut(s) 256
MscI TGGCCA 1 cut(s) 256
MseI TTAA 3 cut(s) 689, 779, 849
MslI CAYNNNNRTG 1 cut(s) 487
Msp20I TGGCCA 1 cut(s) 256
MspI CCGG 2 cut(s) 674, 878
MspR9I CCNGG 3 cut(s) 396, 674, 815
MvaI CCWGG 2 cut(s) 396, 815
MwoI GCNNNNNNNGC 3 cut(s) 262, 498, 701
NciI CCSGG 1 cut(s) 674
NdeII GATC 6 cut(s) 29, 297, 420, 471, 534, 577
NlaIII CATG 3 cut(s) 145, 840, 865
NlaIV GGNNCC 2 cut(s) 616, 678
NmuCI GTSAC 2 cut(s) 156, 539
NspI RCATGY 1 cut(s) 840
NspV TTCGAA 1 cut(s) 240
PagI TCATGA 1 cut(s) 141
PcsI WCGNNNNNNNCGW 2 cut(s) 420, 795
PfeI GAWTC 2 cut(s) 345, 442
PflFI GACNNNGTC 1 cut(s) 587
PinAI ACCGGT 1 cut(s) 877
Ple19I CGATCG 1 cut(s) 32
PleI GAGTC 2 cut(s) 958, 1064
PpsI GAGTC 2 cut(s) 958, 1064
PshBI ATTAAT 1 cut(s) 779
Psp1406I AACGTT 1 cut(s) 659
Psp6I CCWGG 2 cut(s) 394, 813
PspEI GGTNACC 1 cut(s) 791
PspGI CCWGG 2 cut(s) 394, 813
PspN4I GGNNCC 2 cut(s) 616, 678
PspPI GGNCC 3 cut(s) 170, 858, 875
PstNI CAGNNNCTG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 471
PsyI GACNNNGTC 1 cut(s) 587
PvuI CGATCG 1 cut(s) 32
RsaI GTAC 3 cut(s) 458, 735, 765
RsaNI GTAC 3 cut(s) 457, 734, 764
RseI CAYNNNNRTG 1 cut(s) 487
SaqAI TTAA 3 cut(s) 689, 779, 849
Sau3AI GATC 6 cut(s) 29, 297, 420, 471, 534, 577
Sau96I GGNCC 3 cut(s) 170, 858, 875
ScaI AGTACT 1 cut(s) 765
SchI GAGTC 2 cut(s) 959, 1064
ScrFI CCNGG 3 cut(s) 396, 674, 815
SduI GDGCHC 1 cut(s) 39
SfaNI GCATC 1 cut(s) 369
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 1 cut(s) 875
SmiMI CAYNNNNRTG 1 cut(s) 487
SmlI CTYRAG 2 cut(s) 47, 271
SmoI CTYRAG 2 cut(s) 47, 271
Sse9I AATT 6 cut(s) 5, 19, 113, 130, 780, 1044
SspI AATATT 1 cut(s) 567
SspMI CTAG 3 cut(s) 290, 338, 710
StyD4I CCNGG 3 cut(s) 394, 672, 813
StyI CCWWGG 1 cut(s) 961
TaaI ACNGT 2 cut(s) 730, 913
TaiI ACGT 3 cut(s) 642, 662, 874
TaqI TCGA 5 cut(s) 28, 240, 533, 580, 1068
TaqII GACCGA 1 cut(s) 152
TasI AATT 6 cut(s) 5, 19, 113, 130, 780, 1044
TatI WGTACW 2 cut(s) 456, 763
TfiI GAWTC 2 cut(s) 345, 442
Tru1I TTAA 3 cut(s) 689, 779, 849
Tru9I TTAA 3 cut(s) 689, 779, 849
TscAI CASTG 1 cut(s) 831
TseFI GTSAC 2 cut(s) 156, 539
Tsp45I GTSAC 2 cut(s) 156, 539
TspDTI ATGAA 6 cut(s) 74, 199, 400, 455, 765, 878
TspGWI ACGGA 2 cut(s) 80, 943
TspRI CASTG 1 cut(s) 831
Tth111I GACNNNGTC 1 cut(s) 587
VpaK11BI GGWCC 1 cut(s) 875
VspI ATTAAT 1 cut(s) 779
XapI RAATTY 1 cut(s) 19
XbaI TCTAGA 1 cut(s) 337
XceI RCATGY 1 cut(s) 840
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 3 cut(s) 290, 338, 710
ZrmI AGTACT 1 cut(s) 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.