AT5G48480

At5g48480

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011320
Physical Location & Seq
Forward (+)
19644539 .. 19646840
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G48480.1

Sequence Viewer

Length: 501 bp
ATGGCTCAAGAAGATGTTACTGCTGTTGCTACTAACGGTGCTGGTCCGGTGGAGACGCATCTTGTCTTCACTGAGTTCAAGCAGATGTTGCTCGTTGAAGCTCAAAAGGTCGGTGACGCTGTTACTTTCTACAAATCTGCTTTTGGTGCGATCGAGTCTGGTCATTCTCTTTACCCTAAGCGTAAGCTTGACCAAGAGCTTCCTCATGTTCTCTCTTCTGAGCTTAATCTCGCTGGCTCTTCCTTCGTTGTTTGCGACGTTTCCTCTCTCCCTGGTTTTTCTACTGCGAAATCGGAAGGTTCGGGAGTGACTTTTCTTCTCGGAACTAAGGATGCTGAAGCCGCCGTTGCGAAAGCTGTTGACGCCGGAGCTGTGAAAGTGGAGGTTACGGAGGCAGAAGTTGAACTGGGATTCAAAGGAAAAGTTACGGATCCTTTTGGTGTCACTTGGATCTTCGCGGAGAAGAAGACCGTGATCACCGACGAGAACAAAGAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3-dmu-9_3-mt Glyoxalase

Protein Analysis

166

Amino Acids

17.56

Weight (kDa)

4.8

Isoelectric Point (pI)

26.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g48480-like_N PF22656 29 - 87 7.1e-31 Glyoxalase At5g48480-like, N-terminal domain
At5g48480-like_C PF22650 105 - 152 1.1e-26 Glyoxalase At5g48480-like C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48480
fragaria_vesca FvH4_2g17810
malus_domestica MD05G1159600.v1.1 MD10G1152100.v1.1
prunus_persica Prupe.8G190400_v2.0.a1
pyrus_communis pycom05g15120 pycom10g13110
rosa_chinensis RchiOBHm_Chr6g0282841
rosa_laevigata RLG00000012864 RLG00000021743
rosa_multiflora Rmu_sc0006070.1_g000011
rosa_roxburghii Rroxscaffold_7G00185960
rosa_rugosa Rorug02G0530700 Rorug06G0149900
rosa_samantha Rh2BG610300 Rh6AG263200 Rh6BG266100 Rh6CG265300 Rh6DG257900
rosa_wichuraiana Rw6G022720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 458
AciI CCGC 2 cut(s) 342, 458
AclWI GGATC 3 cut(s) 425, 438, 458
AcuI CTGAAG 1 cut(s) 357
AcyI GRCGYC 1 cut(s) 363
AgsI TTSAA 4 cut(s) 79, 98, 404, 415
AjnI CCWGG 1 cut(s) 271
AluBI AGCT 6 cut(s) 101, 187, 199, 223, 356, 371
AluI AGCT 6 cut(s) 101, 187, 199, 223, 356, 371
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 3 cut(s) 425, 438, 458
AspS9I GGNCC 1 cut(s) 44
AsuHPI GGTGA 2 cut(s) 125, 469
AvaII GGWCC 1 cut(s) 44
BamHI GGATCC 1 cut(s) 430
BbsI GAAGAC 2 cut(s) 58, 473
BceAI ACGGC 1 cut(s) 329
BciT130I CCWGG 1 cut(s) 273
BclI TGATCA 1 cut(s) 474
BcoDI GTCTC 1 cut(s) 47
BisI GCNGC 1 cut(s) 342
BlsI GCNGC 1 cut(s) 343
Bme1390I CCNGG 1 cut(s) 273
Bme18I GGWCC 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 1 cut(s) 273
BmrI ACTGGG 1 cut(s) 416
BmsI GCATC 2 cut(s) 67, 322
BmuI ACTGGG 1 cut(s) 416
BpiI GAAGAC 2 cut(s) 58, 473
Bpu10I CCTNAGC 1 cut(s) 177
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 271
BsaWI WCCGGW 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 1 cut(s) 337
BseMII CTCAG 2 cut(s) 63, 210
BseNI ACTGG 1 cut(s) 411
Bsh1236I CGCG 1 cut(s) 458
Bsh1285I CGRYCG 1 cut(s) 153
BsiEI CGRYCG 1 cut(s) 153
BsiSI CCGG 2 cut(s) 47, 366
BsmAI GTCTC 1 cut(s) 47
BsmBI CGTCTC 1 cut(s) 47
Bsp143I GATC 4 cut(s) 150, 430, 450, 474
BspACI CCGC 2 cut(s) 342, 458
BspCNI CTCAG 2 cut(s) 64, 211
BspFNI CGCG 1 cut(s) 458
BspLI GGNNCC 1 cut(s) 432
BspPI GGATC 3 cut(s) 425, 438, 458
BspQI GCTCTTC 1 cut(s) 244
BsrI ACTGG 1 cut(s) 411
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 4 cut(s) 150, 430, 450, 474
BssNI GRCGYC 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 273
Bst4CI ACNGT 2 cut(s) 38, 472
Bst6I CTCTTC 2 cut(s) 220, 244
BstACI GRCGYC 1 cut(s) 363
BstAPI GCANNNNNTGC 1 cut(s) 88
BstC8I GCNNGC 1 cut(s) 235
BstDEI CTNAG 4 cut(s) 72, 177, 219, 327
BstF5I GGATG 1 cut(s) 337
BstFNI CGCG 1 cut(s) 458
BstKTI GATC 4 cut(s) 153, 433, 453, 477
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 4 cut(s) 150, 430, 450, 474
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 5 cut(s) 88, 146, 341, 347, 362
BstNI CCWGG 1 cut(s) 273
BstSCI CCNGG 1 cut(s) 271
BstUI CGCG 1 cut(s) 458
BstV2I GAAGAC 2 cut(s) 58, 473
BstX2I RGATCY 2 cut(s) 430, 450
BstYI RGATCY 2 cut(s) 430, 450
BtsCI GGATG 1 cut(s) 337
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 1 cut(s) 235
Cfr13I GGNCC 1 cut(s) 44
CseI GACGC 3 cut(s) 64, 125, 371
CviAII CATG 1 cut(s) 206
CviJI RGCY 9 cut(s) 5, 101, 187, 199, 223, 237, 341, 356, 371
CviKI_1 RGCY 9 cut(s) 5, 101, 187, 199, 223, 237, 341, 356, 371
DdeI CTNAG 4 cut(s) 72, 177, 219, 327
DpnI GATC 4 cut(s) 152, 432, 452, 476
DpnII GATC 4 cut(s) 150, 430, 450, 474
Eam1104I CTCTTC 2 cut(s) 220, 244
EarI CTCTTC 2 cut(s) 220, 244
Eco47I GGWCC 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 357
EcoRII CCWGG 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 1 cut(s) 209
FaiI YATR 1 cut(s) 207
FatI CATG 1 cut(s) 205
FbaI TGATCA 1 cut(s) 474
Fnu4HI GCNGC 1 cut(s) 342
FokI GGATG 1 cut(s) 344
Fsp4HI GCNGC 1 cut(s) 342
GluI GCNGC 1 cut(s) 342
HapII CCGG 2 cut(s) 47, 366
HgaI GACGC 3 cut(s) 64, 125, 371
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 1 cut(s) 209
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 2 cut(s) 155, 411
HpaII CCGG 2 cut(s) 47, 366
HphI GGTGA 2 cut(s) 125, 469
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 3 cut(s) 220, 295, 323
Hpy188III TCNNGA 2 cut(s) 8, 303
Hpy8I GTNNAC 1 cut(s) 361
Hpy99I CGWCG 2 cut(s) 260, 485
HpyAV CCTTC 2 cut(s) 253, 290
HpyCH4III ACNGT 2 cut(s) 38, 472
HpyCH4IV ACGT 1 cut(s) 258
HpyF10VI GCNNNNNNNGC 5 cut(s) 88, 146, 341, 347, 362
HpyF3I CTNAG 4 cut(s) 72, 177, 219, 327
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 4 cut(s) 150, 430, 450, 474
LguI GCTCTTC 1 cut(s) 244
LmnI GCTCC 1 cut(s) 368
LpnPI CCDG 8 cut(s) 27, 60, 144, 219, 258, 285, 379, 392
LweI GCATC 2 cut(s) 67, 322
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 7 cut(s) 16, 113, 121, 307, 385, 424, 442
MalI GATC 4 cut(s) 152, 432, 452, 476
MboI GATC 4 cut(s) 150, 430, 450, 474
MboII GAAGA 8 cut(s) 23, 58, 207, 231, 308, 445, 475, 478
MflI RGATCY 2 cut(s) 430, 450
MlyI GAGTC 1 cut(s) 164
MnlI CCTC 5 cut(s) 213, 274, 376, 385, 487
MseI TTAA 1 cut(s) 225
MspI CCGG 2 cut(s) 47, 366
MspR9I CCNGG 1 cut(s) 273
MvaI CCWGG 1 cut(s) 273
MvnI CGCG 1 cut(s) 458
MwoI GCNNNNNNNGC 5 cut(s) 88, 146, 341, 347, 362
NdeII GATC 4 cut(s) 150, 430, 450, 474
NlaIII CATG 1 cut(s) 209
NlaIV GGNNCC 1 cut(s) 432
NmuCI GTSAC 3 cut(s) 113, 307, 442
PciSI GCTCTTC 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PfeI GAWTC 1 cut(s) 411
PkrI GCNGC 1 cut(s) 343
Ple19I CGATCG 1 cut(s) 153
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 271
PspGI CCWGG 1 cut(s) 271
PspN4I GGNNCC 1 cut(s) 432
PspPI GGNCC 1 cut(s) 44
PsuI RGATCY 2 cut(s) 430, 450
PvuI CGATCG 1 cut(s) 153
SapI GCTCTTC 1 cut(s) 244
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 1 cut(s) 342
Sau3AI GATC 4 cut(s) 150, 430, 450, 474
Sau96I GGNCC 1 cut(s) 44
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 273
SfaNI GCATC 2 cut(s) 67, 322
SinI GGWCC 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
SsiI CCGC 2 cut(s) 342, 458
StyD4I CCNGG 1 cut(s) 271
TaaI ACNGT 2 cut(s) 38, 472
TaiI ACGT 1 cut(s) 261
TaqI TCGA 1 cut(s) 153
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 1 cut(s) 411
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 3 cut(s) 113, 307, 442
Tsp45I GTSAC 3 cut(s) 113, 307, 442
TspGWI ACGGA 2 cut(s) 404, 443
TspRI CASTG 1 cut(s) 76
VpaK11BI GGWCC 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.