FvH4_2g17810

At5g48480

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011320
Physical Location & Seq
Forward (+)
15279574 .. 15281135
1562 bp
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UTR
Exon/CDS
Intron
FvH4_2g17810.t1

Sequence Viewer

Length: 546 bp
ATGGCGGACCAGGAGGTTCAGAACGGCGGAGCTGAGAAGGTCACCACTGAGTTCAAGAGCACTGTGGCTTTTGATCTCGTCTCTGTGCTTGGAGTCGAAGCACCCAAGGCTGAAGAGGCCGTTCAGTTCTACAAGGCGGTCTTCGGCGCTGTGGAGGTTGAGCGTGTCGCGAACCCTAAGCGTAAGGCGGACCTGGAGCAACCTCTCCTTCAGGTGCTGCTTCAGTTTGGCTCCGGCAAGGTTTTGGTCGTCGATCGCCTTGAGGATGAGTTAAAGTCTCAGCAAAACAGTTCTTTCCAATTGGTCTCTGAGGAAGATGTTGAAGCTGTGATTAAGAAGGTTGTGGCTGCTGGAGGGATCACAGAGGGTGTTGGTGTGACTGAGGACCATGCCTTCCCTGGAAAGCTTTGCTCTGGCAAAGTGAAGGATCCATTTGGCATTGTTTGGACCATTGTTGGCAACAAGAGCACTACTGCTGCTTCTGAGTTTGGGGATAACAACACCTCTGCGGCCGAGTTTGGGCAGAACAACACCAATGAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3-dmu-9_3-mt Glyoxalase

Protein Analysis

182

Amino Acids

19.28

Weight (kDa)

4.59

Isoelectric Point (pI)

25.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g48480-like_N PF22656 30 - 85 2e-19 Glyoxalase At5g48480-like, N-terminal domain
3-dmu-9_3-mt PF06983 36 - 152 8.9e-06 3-demethylubiquinone-9 3-methyltransferase
At5g48480-like_C PF22650 104 - 150 1e-05 Glyoxalase At5g48480-like C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48480
fragaria_vesca FvH4_2g17810
malus_domestica MD05G1159600.v1.1 MD10G1152100.v1.1
prunus_persica Prupe.8G190400_v2.0.a1
pyrus_communis pycom05g15120 pycom10g13110
rosa_chinensis RchiOBHm_Chr6g0282841
rosa_laevigata RLG00000012864 RLG00000021743
rosa_multiflora Rmu_sc0006070.1_g000011
rosa_roxburghii Rroxscaffold_7G00185960
rosa_rugosa Rorug02G0530700 Rorug06G0149900
rosa_samantha Rh2BG610300 Rh6AG263200 Rh6BG266100 Rh6CG265300 Rh6DG257900
rosa_wichuraiana Rw6G022720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 170
AciI CCGC 5 cut(s) 5, 27, 137, 188, 509
AclWI GGATC 3 cut(s) 365, 422, 435
AcoI YGGCCR 1 cut(s) 510
AcuI CTGAAG 3 cut(s) 132, 194, 206
AfiI CCNNNNNNNGG 1 cut(s) 519
AgsI TTSAA 2 cut(s) 55, 323
AjnI CCWGG 3 cut(s) 9, 192, 397
AluBI AGCT 4 cut(s) 32, 326, 406, 542
AluI AGCT 4 cut(s) 32, 326, 406, 542
Alw21I GWGCWC 2 cut(s) 62, 470
Alw26I GTCTC 3 cut(s) 85, 282, 310
AlwI GGATC 3 cut(s) 365, 422, 435
AlwNI CAGNNNCTG 1 cut(s) 217
AoxI GGCC 2 cut(s) 117, 510
ApeKI GCWGC 3 cut(s) 217, 347, 476
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 4 cut(s) 7, 190, 385, 447
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 4 cut(s) 7, 190, 385, 447
BamHI GGATCC 1 cut(s) 427
BbsI GAAGAC 1 cut(s) 133
Bbv12I GWGCWC 2 cut(s) 62, 470
BbvI GCAGC 3 cut(s) 204, 334, 463
BceAI ACGGC 2 cut(s) 40, 104
BciT130I CCWGG 3 cut(s) 11, 194, 399
BcoDI GTCTC 3 cut(s) 85, 282, 310
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 4 cut(s) 218, 348, 477, 510
BlsI GCNGC 4 cut(s) 219, 349, 478, 511
Bme1390I CCNGG 3 cut(s) 11, 194, 399
Bme18I GGWCC 4 cut(s) 7, 190, 385, 447
BmgT120I GGNCC 4 cut(s) 7, 190, 385, 447
BmiI GGNNCC 2 cut(s) 232, 429
BmrFI CCNGG 3 cut(s) 11, 194, 399
BpiI GAAGAC 1 cut(s) 133
BpmI CTGGAG 2 cut(s) 215, 372
Bpu10I CCTNAGC 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 2 cut(s) 105, 397
Bsc4I CCNNNNNNNGG 1 cut(s) 519
BseBI CCWGG 3 cut(s) 11, 194, 399
BseDI CCNNGG 2 cut(s) 105, 397
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 1 cut(s) 519
BseMII CTCAG 6 cut(s) 24, 39, 293, 300, 372, 474
BseX3I CGGCCG 1 cut(s) 510
BseXI GCAGC 3 cut(s) 204, 334, 463
Bsh1236I CGCG 1 cut(s) 170
Bsh1285I CGRYCG 2 cut(s) 256, 513
BshFI GGCC 2 cut(s) 119, 512
BsiEI CGRYCG 2 cut(s) 256, 513
BsiHKAI GWGCWC 2 cut(s) 62, 470
BsiSI CCGG 1 cut(s) 234
BslI CCNNNNNNNGG 1 cut(s) 519
BsmAI GTCTC 3 cut(s) 85, 282, 310
BsmBI CGTCTC 1 cut(s) 85
BsnI GGCC 2 cut(s) 119, 512
Bso31I GGTCTC 1 cut(s) 310
Bsp1286I GDGCHC 2 cut(s) 62, 470
Bsp143I GATC 4 cut(s) 73, 253, 357, 427
Bsp68I TCGCGA 1 cut(s) 170
BspACI CCGC 5 cut(s) 5, 27, 137, 188, 509
BspANI GGCC 2 cut(s) 119, 512
BspCNI CTCAG 6 cut(s) 25, 40, 292, 301, 373, 475
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 2 cut(s) 232, 429
BspPI GGATC 3 cut(s) 365, 422, 435
BspTNI GGTCTC 1 cut(s) 310
BssECI CCNNGG 2 cut(s) 105, 397
BssMI GATC 4 cut(s) 73, 253, 357, 427
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 3 cut(s) 11, 194, 399
Bst4CI ACNGT 2 cut(s) 64, 290
Bst6I CTCTTC 1 cut(s) 108
BstDEI CTNAG 8 cut(s) 33, 48, 177, 279, 309, 381, 483, 543
BstEII GGTNACC 1 cut(s) 40
BstF5I GGATG 1 cut(s) 271
BstFNI CGCG 1 cut(s) 170
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 4 cut(s) 76, 256, 360, 430
BstMAI GTCTC 3 cut(s) 85, 282, 310
BstMBI GATC 4 cut(s) 73, 253, 357, 427
BstMCI CGRYCG 2 cut(s) 256, 513
BstMWI GCNNNNNNNGC 3 cut(s) 107, 116, 465
BstNI CCWGG 3 cut(s) 11, 194, 399
BstPI GGTNACC 1 cut(s) 40
BstSCI CCNGG 3 cut(s) 9, 192, 397
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 3 cut(s) 204, 334, 463
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BstZI CGGCCG 1 cut(s) 510
BsuRI GGCC 2 cut(s) 119, 512
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 45, 60
BtuMI TCGCGA 1 cut(s) 170
CaiI CAGNNNCTG 1 cut(s) 217
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 4 cut(s) 7, 190, 385, 447
CviAII CATG 1 cut(s) 389
DdeI CTNAG 8 cut(s) 33, 48, 177, 279, 309, 381, 483, 543
DpnI GATC 4 cut(s) 75, 255, 359, 429
DpnII GATC 4 cut(s) 73, 253, 357, 427
EaeI YGGCCR 1 cut(s) 510
EagI CGGCCG 1 cut(s) 510
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
EciI GGCGGA 3 cut(s) 20, 42, 203
EclXI CGGCCG 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 105
Eco31I GGTCTC 1 cut(s) 310
Eco47I GGWCC 4 cut(s) 7, 190, 385, 447
Eco52I CGGCCG 1 cut(s) 510
Eco57I CTGAAG 3 cut(s) 132, 194, 206
Eco91I GGTNACC 1 cut(s) 40
EcoO65I GGTNACC 1 cut(s) 40
EcoRII CCWGG 3 cut(s) 9, 192, 397
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
Esp3I CGTCTC 1 cut(s) 85
FaeI CATG 1 cut(s) 392
FaiI YATR 1 cut(s) 390
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FatI CATG 1 cut(s) 388
Fnu4HI GCNGC 4 cut(s) 218, 348, 477, 510
FokI GGATG 1 cut(s) 278
Fsp4HI GCNGC 4 cut(s) 218, 348, 477, 510
GlaI GCGC 1 cut(s) 148
GluI GCNGC 4 cut(s) 218, 348, 477, 510
GsuI CTGGAG 2 cut(s) 215, 372
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 2 cut(s) 119, 512
HapII CCGG 1 cut(s) 234
HhaI GCGC 1 cut(s) 149
Hin1II CATG 1 cut(s) 392
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HindIII AAGCTT 2 cut(s) 404, 540
HinfI GANTC 1 cut(s) 93
HpaII CCGG 1 cut(s) 234
HphI GGTGA 1 cut(s) 34
Hpy188I TCNGA 3 cut(s) 21, 310, 484
Hpy188III TCNNGA 2 cut(s) 55, 169
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 5 cut(s) 31, 218, 331, 403, 418
HpyCH4III ACNGT 2 cut(s) 64, 290
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 116, 465
HpyF3I CTNAG 8 cut(s) 33, 48, 177, 279, 309, 381, 483, 543
Hsp92II CATG 1 cut(s) 392
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 4 cut(s) 73, 253, 357, 427
LmnI GCTCC 3 cut(s) 29, 196, 236
LpnPI CCDG 9 cut(s) 23, 179, 197, 206, 247, 336, 384, 399, 411
Lsp1109I GCAGC 3 cut(s) 204, 334, 463
MaeIII GTNAC 2 cut(s) 40, 376
MalI GATC 4 cut(s) 75, 255, 359, 429
MboI GATC 4 cut(s) 73, 253, 357, 427
MboII GAAGA 3 cut(s) 125, 133, 326
MfeI CAATTG 1 cut(s) 299
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 2 cut(s) 62, 470
MluCI AATT 1 cut(s) 299
MlyI GAGTC 1 cut(s) 102
MseI TTAA 2 cut(s) 272, 333
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 3 cut(s) 11, 194, 399
MunI CAATTG 1 cut(s) 299
MvaI CCWGG 3 cut(s) 11, 194, 399
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 3 cut(s) 107, 116, 465
NdeII GATC 4 cut(s) 73, 253, 357, 427
NlaIII CATG 1 cut(s) 392
NlaIV GGNNCC 2 cut(s) 232, 429
NmeAIII GCCGAG 1 cut(s) 538
NmuCI GTSAC 2 cut(s) 40, 376
NruI TCGCGA 1 cut(s) 170
PkrI GCNGC 4 cut(s) 219, 349, 478, 511
Ple19I CGATCG 1 cut(s) 256
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
Psp6I CCWGG 3 cut(s) 9, 192, 397
PspEI GGTNACC 1 cut(s) 40
PspGI CCWGG 3 cut(s) 9, 192, 397
PspN4I GGNNCC 2 cut(s) 232, 429
PspPI GGNCC 4 cut(s) 7, 190, 385, 447
PstNI CAGNNNCTG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 427
PvuI CGATCG 1 cut(s) 256
RruI TCGCGA 1 cut(s) 170
SaqAI TTAA 2 cut(s) 272, 333
SatI GCNGC 4 cut(s) 218, 348, 477, 510
Sau3AI GATC 4 cut(s) 73, 253, 357, 427
Sau96I GGNCC 4 cut(s) 7, 190, 385, 447
SchI GAGTC 1 cut(s) 102
ScrFI CCNGG 3 cut(s) 11, 194, 399
SduI GDGCHC 2 cut(s) 62, 470
SinI GGWCC 4 cut(s) 7, 190, 385, 447
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 1 cut(s) 299
SsiI CCGC 5 cut(s) 5, 27, 137, 188, 509
StyD4I CCNGG 3 cut(s) 9, 192, 397
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 2 cut(s) 64, 290
TaqI TCGA 2 cut(s) 96, 252
TasI AATT 1 cut(s) 299
TauI GCSGC 1 cut(s) 512
Tru1I TTAA 2 cut(s) 272, 333
Tru9I TTAA 2 cut(s) 272, 333
TscAI CASTG 2 cut(s) 52, 67
TseFI GTSAC 2 cut(s) 40, 376
TseI GCWGC 3 cut(s) 217, 347, 476
Tsp45I GTSAC 2 cut(s) 40, 376
TspRI CASTG 2 cut(s) 52, 67
VpaK11BI GGWCC 4 cut(s) 7, 190, 385, 447
XcmI CCANNNNNNNNNTGG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.