RLG00000021743

At5g48480

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011320
Physical Location & Seq
Forward (+)
77867784 .. 77868526
743 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021743

Sequence Viewer

Length: 432 bp
ATGGACGAGGATGAAGGGAGCTCTCGGTTGACACCCATGGAGGCGTGGACAACGCCTAGCGTGGAAGCTCCAAAGGCTGGGCTCACCGCCGACTTCTACAAGAACGTGTTCGGCTCCATCCAGACTTCCTGTTTCACATTCCGACACCGTGGAGAGAATCCTCTTCTTCTTTGTGTTGTGTTGCTGTTTGGCACAATGAAGATAAACATATCCGATGAGATCGATAGTGGTGATGGTTCCAAGCATGGTGGTATCAGATTGATGTCTAATCAAGATTTGAAAGAAGTTGTTGCTAAGGCGGAGCGTGCTGGAGCTGTATCTGAGGGAGATTTCAGTGAAGACTATATGACGCCAGGCAAGCATATGGGAAAGATTAGGGACCCATTTGGCATAGTGTGGTCTATTGTGGCAAACTTGACTGCTCCCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3-dmu-9_3-mt Glyoxalase

Protein Analysis

144

Amino Acids

15.6

Weight (kDa)

4.99

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48480
fragaria_vesca FvH4_2g17810
malus_domestica MD05G1159600.v1.1 MD10G1152100.v1.1
prunus_persica Prupe.8G190400_v2.0.a1
pyrus_communis pycom05g15120 pycom10g13110
rosa_chinensis RchiOBHm_Chr6g0282841
rosa_laevigata RLG00000012864 RLG00000021743
rosa_multiflora Rmu_sc0006070.1_g000011
rosa_roxburghii Rroxscaffold_7G00185960
rosa_rugosa Rorug02G0530700 Rorug06G0149900
rosa_samantha Rh2BG610300 Rh6AG263200 Rh6BG266100 Rh6CG265300 Rh6DG257900
rosa_wichuraiana Rw6G022720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 77
AciI CCGC 2 cut(s) 87, 299
AcyI GRCGYC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 77
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 1 cut(s) 280
AjnI CCWGG 1 cut(s) 352
AluBI AGCT 3 cut(s) 21, 68, 314
AluI AGCT 3 cut(s) 21, 68, 314
Alw21I GWGCWC 1 cut(s) 23
Asp700I GAANNNNTTC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 379
AsuHPI GGTGA 2 cut(s) 76, 242
AvaII GGWCC 1 cut(s) 379
BanII GRGCYC 2 cut(s) 23, 84
BbsI GAAGAC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 23
BccI CCATC 2 cut(s) 125, 227
BciT130I CCWGG 1 cut(s) 354
BfaI CTAG 1 cut(s) 57
Bme1390I CCNGG 1 cut(s) 354
Bme18I GGWCC 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 379
BmiI GGNNCC 4 cut(s) 115, 238, 380, 381
BmrFI CCNGG 1 cut(s) 354
BpiI GAAGAC 1 cut(s) 345
BpmI CTGGAG 1 cut(s) 330
Bpu10I CCTNAGC 1 cut(s) 294
Bsa29I ATCGAT 1 cut(s) 222
BsaHI GRCGYC 1 cut(s) 350
BsaJI CCNNGG 2 cut(s) 36, 148
Bsc4I CCNNNNNNNGG 1 cut(s) 77
BseBI CCWGG 1 cut(s) 354
BseCI ATCGAT 1 cut(s) 222
BseDI CCNNGG 2 cut(s) 36, 148
BseGI GGATG 2 cut(s) 16, 117
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMII CTCAG 1 cut(s) 312
BseYI CCCAGC 1 cut(s) 77
BshVI ATCGAT 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 23
BslFI GGGAC 1 cut(s) 392
BslI CCNNNNNNNGG 1 cut(s) 77
BsmFI GGGAC 1 cut(s) 392
Bsp1286I GDGCHC 2 cut(s) 23, 84
Bsp143I GATC 1 cut(s) 219
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 2 cut(s) 87, 299
BspCNI CTCAG 1 cut(s) 313
BspDI ATCGAT 1 cut(s) 222
BspLI GGNNCC 4 cut(s) 115, 238, 380, 381
BssECI CCNNGG 2 cut(s) 36, 148
BssMI GATC 1 cut(s) 219
BssNI GRCGYC 1 cut(s) 350
BssT1I CCWWGG 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 1 cut(s) 149
Bst6I CTCTTC 1 cut(s) 168
BstACI GRCGYC 1 cut(s) 350
BstC8I GCNNGC 2 cut(s) 306, 359
BstDEI CTNAG 2 cut(s) 294, 321
BstDSI CCRYGG 2 cut(s) 36, 148
BstF5I GGATG 2 cut(s) 16, 117
BstKTI GATC 1 cut(s) 222
BstMBI GATC 1 cut(s) 219
BstMWI GCNNNNNNNGC 3 cut(s) 74, 305, 358
BstNI CCWGG 1 cut(s) 354
BstSCI CCNGG 1 cut(s) 352
BstV2I GAAGAC 1 cut(s) 345
Bsu15I ATCGAT 1 cut(s) 222
BsuTUI ATCGAT 1 cut(s) 222
BtgI CCRYGG 2 cut(s) 36, 148
BtsCI GGATG 2 cut(s) 16, 117
BtsIMutI CAGTG 1 cut(s) 340
Cac8I GCNNGC 2 cut(s) 306, 359
Cfr13I GGNCC 1 cut(s) 379
ClaI ATCGAT 1 cut(s) 222
CseI GACGC 1 cut(s) 358
CspCI CAANNNNNGTGG 2 cut(s) 229, 264
CviAII CATG 2 cut(s) 37, 245
CviJI RGCY 6 cut(s) 21, 68, 77, 82, 114, 314
CviKI_1 RGCY 6 cut(s) 21, 68, 77, 82, 114, 314
DdeI CTNAG 2 cut(s) 294, 321
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
EciI GGCGGA 1 cut(s) 314
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 36
Eco24I GRGCYC 2 cut(s) 23, 84
Eco47I GGWCC 1 cut(s) 379
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 36
EcoT38I GRGCYC 2 cut(s) 23, 84
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 2 cut(s) 40, 248
FaiI YATR 8 cut(s) 38, 209, 246, 345, 347, 363, 365, 392
FaqI GGGAC 1 cut(s) 392
FatI CATG 2 cut(s) 36, 244
FauNDI CATATG 1 cut(s) 363
FokI GGATG 2 cut(s) 23, 104
FriOI GRGCYC 2 cut(s) 23, 84
FspBI CTAG 1 cut(s) 57
GsaI CCCAGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 330
HgaI GACGC 1 cut(s) 358
Hin1I GRCGYC 1 cut(s) 350
Hin1II CATG 2 cut(s) 40, 248
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 1 cut(s) 157
HphI GGTGA 2 cut(s) 76, 242
Hpy166II GTNNAC 2 cut(s) 30, 48
Hpy188I TCNGA 4 cut(s) 143, 214, 257, 322
Hpy188III TCNNGA 2 cut(s) 121, 272
Hpy8I GTNNAC 2 cut(s) 30, 48
HpyAV CCTTC 1 cut(s) 8
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4IV ACGT 1 cut(s) 105
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 305, 358
HpyF3I CTNAG 2 cut(s) 294, 321
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 1 cut(s) 350
Hsp92II CATG 2 cut(s) 40, 248
KflI GGGWCCC 1 cut(s) 379
Kzo9I GATC 1 cut(s) 219
LmnI GCTCC 6 cut(s) 18, 73, 119, 301, 311, 427
LpnPI CCDG 6 cut(s) 63, 134, 142, 294, 339, 366
MaeI CTAG 1 cut(s) 57
MaeII ACGT 1 cut(s) 105
MalI GATC 1 cut(s) 221
MboI GATC 1 cut(s) 219
MboII GAAGA 4 cut(s) 155, 158, 211, 350
MhlI GDGCHC 2 cut(s) 23, 84
MmeI TCCRAC 1 cut(s) 166
MnlI CCTC 3 cut(s) 34, 171, 316
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 1 cut(s) 430
MspR9I CCNGG 1 cut(s) 354
MvaI CCWGG 1 cut(s) 354
MwoI GCNNNNNNNGC 3 cut(s) 74, 305, 358
NcoI CCATGG 1 cut(s) 36
NdeI CATATG 1 cut(s) 363
NdeII GATC 1 cut(s) 219
NlaIII CATG 2 cut(s) 40, 248
NlaIV GGNNCC 4 cut(s) 115, 238, 380, 381
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 157
PflMI CCANNNNNTGG 1 cut(s) 77
PpuMI RGGWCCY 1 cut(s) 379
Psp124BI GAGCTC 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 1 cut(s) 352
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 4 cut(s) 115, 238, 380, 381
PspPI GGNCC 1 cut(s) 379
PspPPI RGGWCCY 1 cut(s) 379
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 1 cut(s) 430
Sau3AI GATC 1 cut(s) 219
Sau96I GGNCC 1 cut(s) 379
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 2 cut(s) 23, 84
SetI ASST 4 cut(s) 23, 70, 108, 316
SinI GGWCC 1 cut(s) 379
SsiI CCGC 2 cut(s) 87, 299
SspMI CTAG 1 cut(s) 57
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 352
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 1 cut(s) 149
TaiI ACGT 1 cut(s) 108
TaqI TCGA 1 cut(s) 222
TfiI GAWTC 1 cut(s) 157
Tru1I TTAA 1 cut(s) 430
Tru9I TTAA 1 cut(s) 430
TscAI CASTG 1 cut(s) 340
TspDTI ATGAA 2 cut(s) 27, 212
TspRI CASTG 1 cut(s) 340
Van91I CCANNNNNTGG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 379
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.