Rw6G022720

At5g48480

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011320
Physical Location & Seq
Forward (+)
44751394 .. 44753302
1909 bp
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UTR
Exon/CDS
Intron
Rw6G022720.1

Sequence Viewer

Length: 711 bp
ATGTATCCCCAAACGGTTGCGCTTCCTGGCTTCTATAAAACCCCTACCCCACCACCAGCGCAAACCCCACGCTCTTTTCATTTTCACCATCACTATCCAGAGCAAGGGTTTTTTCTCTCTCGCCTTTTGTTTTCTCTCTGCGTTTCTCTGTCTGTGAGTGCATCAATGGCGGACCAGGAGGTTCAGAACGGCGGAGCTGAGAAGGTCGCTACTGAGGCCAAGAGTGTGGCTCTCGATCTCCACGCTGTGCTGGGAGTGGAAGCCCCCAAGGCTGACGACGCCGTTCAGTTCTACAAGGCGGTCTTCGGCGCTGTGGAGGTTGAGCGTGGCGCGAACGCCAAGCGTAAGGCGGACCTGGAGCGCCCTCTCATCCATGTGTTGCTTCAGTTTGGCTCCTGCAAGGTTTTGGTCGTTGATCGCCTTGAGGATGAGTTGAAGGCTCATCAAAACAGCACTGGTCTCGCATTGGTCTGTGAGGGGGATATTGAAGCTGTGATCAAGAAGGTTGTGGCTGCTGGAGGGAATACAGAGAGTGGTGGTGTGACTGAGGACCATGCCTTCCCTGGAAAGCTATACTCCGGCAAAGTCAAGGACCCATTCGGCATTGTCTGGACCATTGTTGGCAACAAGACCATCACCGCTGCTTCTGAGACTGGGCCAGAAAACACCACTGCTGCCGAGATTGAGGCAAACAACACCACTGATGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3-dmu-9_3-mt Glyoxalase

Protein Analysis

236

Amino Acids

25.17

Weight (kDa)

5.48

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g48480-like_N PF22656 84 - 139 5e-19 Glyoxalase At5g48480-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48480
fragaria_vesca FvH4_2g17810
malus_domestica MD05G1159600.v1.1 MD10G1152100.v1.1
prunus_persica Prupe.8G190400_v2.0.a1
pyrus_communis pycom05g15120 pycom10g13110
rosa_chinensis RchiOBHm_Chr6g0282841
rosa_laevigata RLG00000012864 RLG00000021743
rosa_multiflora Rmu_sc0006070.1_g000011
rosa_roxburghii Rroxscaffold_7G00185960
rosa_rugosa Rorug02G0530700 Rorug06G0149900
rosa_samantha Rh2BG610300 Rh6AG263200 Rh6BG266100 Rh6CG265300 Rh6DG257900
rosa_wichuraiana Rw6G022720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 332
AciI CCGC 5 cut(s) 170, 192, 299, 350, 639
AcuI CTGAAG 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 279
AdeI CACNNNGTG 1 cut(s) 247
AfiI CCNNNNNNNGG 1 cut(s) 104
AgsI TTSAA 2 cut(s) 436, 488
AjnI CCWGG 4 cut(s) 25, 174, 354, 562
AluBI AGCT 3 cut(s) 197, 491, 571
AluI AGCT 3 cut(s) 197, 491, 571
Alw26I GTCTC 2 cut(s) 464, 644
AoxI GGCC 2 cut(s) 216, 656
ApeKI GCWGC 3 cut(s) 512, 641, 674
AspLEI GCGC 5 cut(s) 22, 61, 311, 332, 363
AspS9I GGNCC 6 cut(s) 172, 352, 550, 592, 612, 656
AsuHPI GGTGA 2 cut(s) 77, 628
AvaII GGWCC 5 cut(s) 172, 352, 550, 592, 612
BbsI GAAGAC 1 cut(s) 295
BbvI GCAGC 3 cut(s) 499, 628, 661
BccI CCATC 2 cut(s) 96, 641
BceAI ACGGC 2 cut(s) 205, 266
BcgI CGANNNNNNTGC 2 cut(s) 442, 476
BciT130I CCWGG 4 cut(s) 27, 176, 356, 564
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 1 cut(s) 495
BcoDI GTCTC 2 cut(s) 464, 644
BfoI RGCGCY 2 cut(s) 312, 364
BfuI GTATCC 1 cut(s) 15
BglI GCCNNNNNGGC 1 cut(s) 269
BisI GCNGC 3 cut(s) 513, 642, 675
BlsI GCNGC 3 cut(s) 514, 643, 676
Bme1390I CCNGG 4 cut(s) 27, 176, 356, 564
Bme18I GGWCC 5 cut(s) 172, 352, 550, 592, 612
BmgT120I GGNCC 6 cut(s) 172, 352, 550, 592, 612, 656
BmiI GGNNCC 2 cut(s) 394, 594
BmrFI CCNGG 4 cut(s) 27, 176, 356, 564
BmrI ACTGGG 1 cut(s) 663
BmsI GCATC 2 cut(s) 170, 694
BmuI ACTGGG 1 cut(s) 663
BpiI GAAGAC 1 cut(s) 295
BplI GAGNNNNNCTC 2 cut(s) 214, 246
BpmI CTGGAG 2 cut(s) 377, 537
BpuEI CTTGAG 1 cut(s) 443
BsaHI GRCGYC 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 464
BsaJI CCNNGG 2 cut(s) 267, 562
Bsc4I CCNNNNNNNGG 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 460, 658
BseBI CCWGG 4 cut(s) 27, 176, 356, 564
BseDI CCNNGG 2 cut(s) 267, 562
BseGI GGATG 2 cut(s) 369, 433
BseLI CCNNNNNNNGG 1 cut(s) 104
BseMII CTCAG 4 cut(s) 189, 204, 537, 639
BseNI ACTGG 2 cut(s) 460, 658
BseXI GCAGC 3 cut(s) 499, 628, 661
BseYI CCCAGC 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 332
BshFI GGCC 2 cut(s) 218, 658
BsiSI CCGG 1 cut(s) 579
BslI CCNNNNNNNGG 1 cut(s) 104
BsmAI GTCTC 2 cut(s) 464, 644
BsnI GGCC 2 cut(s) 218, 658
Bso31I GGTCTC 1 cut(s) 464
Bsp143I GATC 3 cut(s) 235, 415, 495
BspACI CCGC 5 cut(s) 170, 192, 299, 350, 639
BspANI GGCC 2 cut(s) 218, 658
BspCNI CTCAG 4 cut(s) 190, 205, 538, 640
BspFNI CGCG 1 cut(s) 332
BspLI GGNNCC 2 cut(s) 394, 594
BspTNI GGTCTC 1 cut(s) 464
BsrI ACTGG 2 cut(s) 460, 658
BssECI CCNNGG 2 cut(s) 267, 562
BssMI GATC 3 cut(s) 235, 415, 495
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 4 cut(s) 27, 176, 356, 564
Bst4CI ACNGT 1 cut(s) 16
BstACI GRCGYC 1 cut(s) 279
BstDEI CTNAG 4 cut(s) 198, 213, 546, 648
BstF5I GGATG 2 cut(s) 369, 433
BstFNI CGCG 1 cut(s) 332
BstH2I RGCGCY 2 cut(s) 312, 364
BstHHI GCGC 5 cut(s) 22, 61, 311, 332, 363
BstKTI GATC 3 cut(s) 238, 418, 498
BstMAI GTCTC 2 cut(s) 464, 644
BstMBI GATC 3 cut(s) 235, 415, 495
BstMWI GCNNNNNNNGC 4 cut(s) 167, 215, 269, 278
BstNI CCWGG 4 cut(s) 27, 176, 356, 564
BstSCI CCNGG 4 cut(s) 25, 174, 354, 562
BstUI CGCG 1 cut(s) 332
BstV1I GCAGC 3 cut(s) 499, 628, 661
BstV2I GAAGAC 1 cut(s) 295
BstXI CCANNNNNNTGG 1 cut(s) 226
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 2 cut(s) 218, 658
BtsCI GGATG 2 cut(s) 369, 433
BtsI GCAGTG 1 cut(s) 669
BtsIMutI CAGTG 3 cut(s) 453, 669, 699
CfoI GCGC 5 cut(s) 22, 61, 311, 332, 363
Cfr13I GGNCC 6 cut(s) 172, 352, 550, 592, 612, 656
CseI GACGC 1 cut(s) 287
CviAII CATG 2 cut(s) 374, 554
DdeI CTNAG 4 cut(s) 198, 213, 546, 648
DpnI GATC 3 cut(s) 237, 417, 497
DpnII GATC 3 cut(s) 235, 415, 495
DraIII CACNNNGTG 1 cut(s) 247
EciI GGCGGA 3 cut(s) 185, 207, 365
Eco130I CCWWGG 1 cut(s) 267
Eco31I GGTCTC 1 cut(s) 464
Eco47I GGWCC 5 cut(s) 172, 352, 550, 592, 612
Eco57I CTGAAG 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 592
EcoRII CCWGG 4 cut(s) 25, 174, 354, 562
EcoT14I CCWWGG 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 2 cut(s) 377, 557
FaiI YATR 4 cut(s) 36, 375, 555, 574
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FatI CATG 2 cut(s) 373, 553
FbaI TGATCA 1 cut(s) 495
Fnu4HI GCNGC 3 cut(s) 513, 642, 675
FokI GGATG 2 cut(s) 356, 440
Fsp4HI GCNGC 3 cut(s) 513, 642, 675
GlaI GCGC 5 cut(s) 21, 60, 310, 331, 362
GluI GCNGC 3 cut(s) 513, 642, 675
GsaI CCCAGC 1 cut(s) 254
GsuI CTGGAG 2 cut(s) 377, 537
HaeII RGCGCY 2 cut(s) 312, 364
HaeIII GGCC 2 cut(s) 218, 658
HapII CCGG 1 cut(s) 579
HgaI GACGC 1 cut(s) 287
HhaI GCGC 5 cut(s) 22, 61, 311, 332, 363
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 2 cut(s) 377, 557
Hin6I GCGC 5 cut(s) 20, 59, 309, 330, 361
HinP1I GCGC 5 cut(s) 20, 59, 309, 330, 361
HpaII CCGG 1 cut(s) 579
HphI GGTGA 2 cut(s) 77, 628
Hpy188I TCNGA 2 cut(s) 186, 649
Hpy188III TCNNGA 4 cut(s) 98, 233, 499, 610
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 4 cut(s) 196, 430, 496, 568
HpyCH4III ACNGT 1 cut(s) 16
HpyCH4V TGCA 2 cut(s) 161, 399
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 215, 269, 278
HpyF3I CTNAG 4 cut(s) 198, 213, 546, 648
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 2 cut(s) 377, 557
HspAI GCGC 5 cut(s) 20, 59, 309, 330, 361
Ksp22I TGATCA 1 cut(s) 495
Kzo9I GATC 3 cut(s) 235, 415, 495
LmnI GCTCC 3 cut(s) 194, 358, 398
Lsp1109I GCAGC 3 cut(s) 499, 628, 661
LweI GCATC 2 cut(s) 170, 694
MaeIII GTNAC 1 cut(s) 541
MalI GATC 3 cut(s) 237, 417, 497
MboI GATC 3 cut(s) 235, 415, 495
MboII GAAGA 1 cut(s) 295
MnlI CCTC 9 cut(s) 172, 208, 310, 375, 418, 469, 512, 541, 679
MslI CAYNNNNRTG 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 641
MspI CCGG 1 cut(s) 579
MspR9I CCNGG 4 cut(s) 27, 176, 356, 564
MvaI CCWGG 4 cut(s) 27, 176, 356, 564
MvnI CGCG 1 cut(s) 332
MwoI GCNNNNNNNGC 4 cut(s) 167, 215, 269, 278
NdeII GATC 3 cut(s) 235, 415, 495
NlaIII CATG 2 cut(s) 377, 557
NlaIV GGNNCC 2 cut(s) 394, 594
NmeAIII GCCGAG 1 cut(s) 703
NmuCI GTSAC 1 cut(s) 541
PkrI GCNGC 3 cut(s) 514, 643, 676
PpuMI RGGWCCY 1 cut(s) 592
Psp5II RGGWCCY 1 cut(s) 592
Psp6I CCWGG 4 cut(s) 25, 174, 354, 562
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 4 cut(s) 25, 174, 354, 562
PspN4I GGNNCC 2 cut(s) 394, 594
PspPI GGNCC 6 cut(s) 172, 352, 550, 592, 612, 656
PspPPI RGGWCCY 1 cut(s) 592
RseI CAYNNNNRTG 1 cut(s) 374
SatI GCNGC 3 cut(s) 513, 642, 675
Sau3AI GATC 3 cut(s) 235, 415, 495
Sau96I GGNCC 6 cut(s) 172, 352, 550, 592, 612, 656
ScrFI CCNGG 4 cut(s) 27, 176, 356, 564
SetI ASST 9 cut(s) 183, 199, 207, 321, 357, 405, 493, 507, 573
SfaNI GCATC 2 cut(s) 170, 694
SinI GGWCC 5 cut(s) 172, 352, 550, 592, 612
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SsiI CCGC 5 cut(s) 170, 192, 299, 350, 639
StyD4I CCNGG 4 cut(s) 25, 174, 354, 562
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 234
TscAI CASTG 3 cut(s) 460, 676, 706
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 3 cut(s) 512, 641, 674
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 1 cut(s) 68
TspRI CASTG 3 cut(s) 460, 676, 706
VpaK11BI GGWCC 5 cut(s) 172, 352, 550, 592, 612
XcmI CCANNNNNNNNNTGG 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.