MD05G1159600.v1.1

At5g48480

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011320
Physical Location & Seq
Forward (+)
28822413 .. 28823424
1012 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1159600.v1.1.491

Sequence Viewer

Length: 537 bp
ATGGCGGAGCAAAAGGTCCAGAACGGTGAGGCCGCGGAGAAGGTCGCACCCGTGTCCCTTATCACTGTGACGGAGTGCCATCCTCGTCTCTCGATCAAGGCACCCAATGGCGAAGATGCCATCAAGTTCTACGTGGCCGTCCTGGGCGCCGTGGAGCTTGAGCGTGAGACTGATCTCAAGCGCAAGGCCGACCAGGAGCGTCCGTACATCTACAATGCGAAGCTCAAGCTTGGGTCCCTCACCTTCCTTGTGACTGAGTCTGCCTCGGTGGTCGACTCCGGCGACGGCGAAGTTCTAGCTGACGGTGGTTTTAAGCAAGGCGTTAATCTGCTACTTACTGTTGCGGACGTCGATGCGGCGGTGTCCAAGGTCGTCCAGGCGGGAGCTGCAGCGGTTGGAGAGATCAAAACCGGTGGTGCTTTCTTTGGCGACGAGCAGCGTTGGGCTCAGATTAAGGATCCATTCGGCATTTGCTGGTTCTTTGTCACTCCCGTTGAGAAGAAGGCCGCTGTCCCTGAAGCCCCGGTTACCGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3-dmu-9_3-mt Glyoxalase

Protein Analysis

179

Amino Acids

18.95

Weight (kDa)

4.91

Isoelectric Point (pI)

28.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g48480-like_N PF22656 30 - 85 5.3e-17 Glyoxalase At5g48480-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48480
fragaria_vesca FvH4_2g17810
malus_domestica MD05G1159600.v1.1 MD10G1152100.v1.1
prunus_persica Prupe.8G190400_v2.0.a1
pyrus_communis pycom05g15120 pycom10g13110
rosa_chinensis RchiOBHm_Chr6g0282841
rosa_laevigata RLG00000012864 RLG00000021743
rosa_multiflora Rmu_sc0006070.1_g000011
rosa_roxburghii Rroxscaffold_7G00185960
rosa_rugosa Rorug02G0530700 Rorug06G0149900
rosa_samantha Rh2BG610300 Rh6AG263200 Rh6BG266100 Rh6CG265300 Rh6DG257900
rosa_wichuraiana Rw6G022720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 351
AccB1I GGYRCC 2 cut(s) 100, 146
AccI GTMKAC 1 cut(s) 273
AccII CGCG 1 cut(s) 35
AciI CCGC 9 cut(s) 5, 33, 35, 344, 356, 359, 380, 392, 507
AclWI GGATC 2 cut(s) 452, 465
AcoI YGGCCR 1 cut(s) 135
AcuI CTGAAG 1 cut(s) 537
AcyI GRCGYC 2 cut(s) 147, 348
AfaI GTAC 1 cut(s) 206
AgeI ACCGGT 1 cut(s) 410
AjnI CCWGG 3 cut(s) 141, 192, 375
AluBI AGCT 5 cut(s) 157, 223, 229, 299, 386
AluI AGCT 5 cut(s) 157, 223, 229, 299, 386
Alw26I GTCTC 2 cut(s) 92, 161
AlwI GGATC 2 cut(s) 452, 465
AoxI GGCC 4 cut(s) 30, 135, 186, 504
ApeKI GCWGC 3 cut(s) 386, 389, 436
AsiGI ACCGGT 1 cut(s) 410
AspLEI GCGC 2 cut(s) 149, 183
AspS9I GGNCC 2 cut(s) 16, 234
AsuC2I CCSGG 1 cut(s) 524
AsuHPI GGTGA 2 cut(s) 38, 232
AvaII GGWCC 2 cut(s) 16, 234
BamHI GGATCC 1 cut(s) 457
BanI GGYRCC 2 cut(s) 100, 146
BanII GRGCYC 1 cut(s) 448
BbvI GCAGC 3 cut(s) 373, 401, 448
BccI CCATC 2 cut(s) 87, 128
BceAI ACGGC 3 cut(s) 122, 134, 301
BciT130I CCWGG 3 cut(s) 143, 194, 377
BcnI CCSGG 1 cut(s) 524
BcoDI GTCTC 2 cut(s) 92, 161
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 387
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 6 cut(s) 33, 357, 387, 390, 437, 507
BlsI GCNGC 6 cut(s) 34, 358, 388, 391, 438, 508
Bme1390I CCNGG 4 cut(s) 143, 194, 377, 524
Bme18I GGWCC 2 cut(s) 16, 234
BmgT120I GGNCC 2 cut(s) 16, 234
BmiI GGNNCC 5 cut(s) 102, 148, 235, 236, 459
BmrFI CCNGG 4 cut(s) 143, 194, 377, 524
BmsI GCATC 2 cut(s) 106, 343
BplI GAGNNNNNCTC 2 cut(s) 248, 280
BpuEI CTTGAG 3 cut(s) 161, 179, 209
BpuMI CCSGG 1 cut(s) 524
BsaAI YACGTR 1 cut(s) 133
BsaHI GRCGYC 2 cut(s) 147, 348
BsaJI CCNNGG 6 cut(s) 33, 142, 150, 264, 366, 522
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bse118I RCCGGY 1 cut(s) 410
BseBI CCWGG 3 cut(s) 143, 194, 377
BseDI CCNNGG 6 cut(s) 33, 142, 150, 264, 366, 522
BseGI GGATG 1 cut(s) 79
BseMII CTCAG 2 cut(s) 246, 461
BseXI GCAGC 3 cut(s) 373, 401, 448
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 4 cut(s) 32, 137, 188, 506
BshNI GGYRCC 2 cut(s) 100, 146
BshTI ACCGGT 1 cut(s) 410
BsiSI CCGG 3 cut(s) 279, 411, 524
BslFI GGGAC 3 cut(s) 40, 220, 497
BsmAI GTCTC 2 cut(s) 92, 161
BsmBI CGTCTC 1 cut(s) 92
BsmFI GGGAC 3 cut(s) 40, 220, 497
BsnI GGCC 4 cut(s) 32, 137, 188, 506
Bsp1286I GDGCHC 1 cut(s) 448
Bsp143I GATC 4 cut(s) 93, 172, 402, 457
BspACI CCGC 9 cut(s) 5, 33, 35, 344, 356, 359, 380, 392, 507
BspANI GGCC 4 cut(s) 32, 137, 188, 506
BspCNI CTCAG 2 cut(s) 247, 460
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 5 cut(s) 102, 148, 235, 236, 459
BspMAI CTGCAG 1 cut(s) 391
BspPI GGATC 2 cut(s) 452, 465
BspT107I GGYRCC 2 cut(s) 100, 146
BsrFI RCCGGY 1 cut(s) 410
BssAI RCCGGY 1 cut(s) 410
BssECI CCNNGG 6 cut(s) 33, 142, 150, 264, 366, 522
BssMI GATC 4 cut(s) 93, 172, 402, 457
BssNI GRCGYC 2 cut(s) 147, 348
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 3 cut(s) 143, 194, 377
Bst4CI ACNGT 5 cut(s) 26, 67, 305, 340, 532
BstACI GRCGYC 2 cut(s) 147, 348
BstBAI YACGTR 1 cut(s) 133
BstDEI CTNAG 2 cut(s) 255, 447
BstDSI CCRYGG 2 cut(s) 33, 150
BstEII GGTNACC 1 cut(s) 526
BstF5I GGATG 1 cut(s) 79
BstFNI CGCG 1 cut(s) 35
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 2 cut(s) 149, 183
BstKTI GATC 4 cut(s) 96, 175, 405, 460
BstMAI GTCTC 2 cut(s) 92, 161
BstMBI GATC 4 cut(s) 93, 172, 402, 457
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNI CCWGG 3 cut(s) 143, 194, 377
BstPI GGTNACC 1 cut(s) 526
BstSCI CCNGG 4 cut(s) 141, 192, 375, 522
BstSFI CTRYAG 1 cut(s) 387
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 373, 401, 448
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
BsuRI GGCC 4 cut(s) 32, 137, 188, 506
BtgI CCRYGG 2 cut(s) 33, 150
BtsCI GGATG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 63
CfoI GCGC 2 cut(s) 149, 183
Cfr10I RCCGGY 1 cut(s) 410
Cfr13I GGNCC 2 cut(s) 16, 234
Cfr42I CCGCGG 1 cut(s) 36
CseI GACGC 1 cut(s) 188
Csp6I GTAC 1 cut(s) 205
CspAI ACCGGT 1 cut(s) 410
CspCI CAANNNNNGTGG 2 cut(s) 394, 429
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 2 cut(s) 255, 447
DinI GGCGCC 1 cut(s) 148
DpnI GATC 4 cut(s) 95, 174, 404, 459
DpnII GATC 4 cut(s) 93, 172, 402, 457
EaeI YGGCCR 1 cut(s) 135
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 448
Eco47I GGWCC 2 cut(s) 16, 234
Eco57I CTGAAG 1 cut(s) 537
Eco91I GGTNACC 1 cut(s) 526
EcoO109I RGGNCCY 1 cut(s) 234
EcoO65I GGTNACC 1 cut(s) 526
EcoRII CCWGG 3 cut(s) 141, 192, 375
EcoT14I CCWWGG 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 448
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 366
Esp3I CGTCTC 1 cut(s) 92
FaqI GGGAC 3 cut(s) 40, 220, 497
FauI CCCGC 1 cut(s) 373
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 6 cut(s) 33, 357, 387, 390, 437, 507
FokI GGATG 1 cut(s) 66
FriOI GRGCYC 1 cut(s) 448
Fsp4HI GCNGC 6 cut(s) 33, 357, 387, 390, 437, 507
FspBI CTAG 1 cut(s) 296
GlaI GCGC 2 cut(s) 148, 182
GluI GCNGC 6 cut(s) 33, 357, 387, 390, 437, 507
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 4 cut(s) 32, 137, 188, 506
HapII CCGG 3 cut(s) 279, 411, 524
HgaI GACGC 1 cut(s) 188
HhaI GCGC 2 cut(s) 149, 183
Hin1I GRCGYC 2 cut(s) 147, 348
Hin6I GCGC 2 cut(s) 147, 181
HinP1I GCGC 2 cut(s) 147, 181
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 2 cut(s) 257, 275
HpaII CCGG 3 cut(s) 279, 411, 524
HphI GGTGA 2 cut(s) 38, 232
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 1 cut(s) 450
Hpy188III TCNNGA 2 cut(s) 19, 91
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 3 cut(s) 287, 353, 434
HpyAV CCTTC 3 cut(s) 34, 253, 496
HpyCH4III ACNGT 5 cut(s) 26, 67, 305, 340, 532
HpyCH4IV ACGT 2 cut(s) 132, 348
HpyCH4V TGCA 1 cut(s) 389
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 2 cut(s) 255, 447
HpySE526I ACGT 2 cut(s) 132, 348
Hsp92I GRCGYC 2 cut(s) 147, 348
HspAI GCGC 2 cut(s) 147, 181
KasI GGCGCC 1 cut(s) 146
KflI GGGWCCC 1 cut(s) 234
KspI CCGCGG 1 cut(s) 36
Kzo9I GATC 4 cut(s) 93, 172, 402, 457
LmnI GCTCC 4 cut(s) 7, 154, 196, 383
Lsp1109I GCAGC 3 cut(s) 373, 401, 448
LweI GCATC 2 cut(s) 106, 343
MaeI CTAG 1 cut(s) 296
MaeII ACGT 2 cut(s) 132, 348
MaeIII GTNAC 4 cut(s) 67, 250, 484, 526
MalI GATC 4 cut(s) 95, 174, 404, 459
MboI GATC 4 cut(s) 93, 172, 402, 457
MboII GAAGA 2 cut(s) 125, 511
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 1 cut(s) 448
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 2 cut(s) 266, 269
MmeI TCCRAC 1 cut(s) 376
MnlI CCTC 4 cut(s) 22, 93, 248, 274
MseI TTAA 3 cut(s) 312, 324, 453
MspA1I CMGCKG 3 cut(s) 35, 392, 509
MspI CCGG 3 cut(s) 279, 411, 524
MspR9I CCNGG 4 cut(s) 143, 194, 377, 524
MvaI CCWGG 3 cut(s) 143, 194, 377
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 1 cut(s) 386
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 1 cut(s) 524
NdeII GATC 4 cut(s) 93, 172, 402, 457
NlaIV GGNNCC 5 cut(s) 102, 148, 235, 236, 459
NmuCI GTSAC 3 cut(s) 67, 250, 484
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PflFI GACNNNGTC 1 cut(s) 256
PinAI ACCGGT 1 cut(s) 410
PkrI GCNGC 6 cut(s) 34, 358, 388, 391, 438, 508
PleI GAGTC 2 cut(s) 265, 269
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 2 cut(s) 265, 269
Ppu21I YACGTR 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 234
Psp6I CCWGG 3 cut(s) 141, 192, 375
PspEI GGTNACC 1 cut(s) 526
PspGI CCWGG 3 cut(s) 141, 192, 375
PspN4I GGNNCC 5 cut(s) 102, 148, 235, 236, 459
PspPI GGNCC 2 cut(s) 16, 234
PspPPI RGGWCCY 1 cut(s) 234
PstI CTGCAG 1 cut(s) 391
PsuI RGATCY 1 cut(s) 457
PsyI GACNNNGTC 1 cut(s) 256
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SacII CCGCGG 1 cut(s) 36
SalI GTCGAC 1 cut(s) 272
SaqAI TTAA 3 cut(s) 312, 324, 453
SatI GCNGC 6 cut(s) 33, 357, 387, 390, 437, 507
Sau3AI GATC 4 cut(s) 93, 172, 402, 457
Sau96I GGNCC 2 cut(s) 16, 234
SchI GAGTC 2 cut(s) 266, 269
ScrFI CCNGG 4 cut(s) 143, 194, 377, 524
SduI GDGCHC 1 cut(s) 448
SfaNI GCATC 2 cut(s) 106, 343
SfcI CTRYAG 1 cut(s) 387
SfoI GGCGCC 1 cut(s) 148
Sfr303I CCGCGG 1 cut(s) 36
SgrBI CCGCGG 1 cut(s) 36
SinI GGWCC 2 cut(s) 16, 234
SmlI CTYRAG 3 cut(s) 158, 176, 224
SmoI CTYRAG 3 cut(s) 158, 176, 224
SsiI CCGC 9 cut(s) 5, 33, 35, 344, 356, 359, 380, 392, 507
SspDI GGCGCC 1 cut(s) 146
SspMI CTAG 1 cut(s) 296
StyD4I CCNGG 4 cut(s) 141, 192, 375, 522
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 5 cut(s) 26, 67, 305, 340, 532
TaiI ACGT 2 cut(s) 135, 351
TaqI TCGA 3 cut(s) 92, 273, 351
TauI GCSGC 3 cut(s) 35, 359, 509
Tru1I TTAA 3 cut(s) 312, 324, 453
Tru9I TTAA 3 cut(s) 312, 324, 453
TscAI CASTG 1 cut(s) 70
TseFI GTSAC 3 cut(s) 67, 250, 484
TseI GCWGC 3 cut(s) 386, 389, 436
Tsp45I GTSAC 3 cut(s) 67, 250, 484
TspGWI ACGGA 2 cut(s) 86, 192
TspRI CASTG 1 cut(s) 70
Tth111I GACNNNGTC 1 cut(s) 256
VpaK11BI GGWCC 2 cut(s) 16, 234
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 1 cut(s) 296
ZraI GACGTC 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.