RLG00000012864

At5g48480

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011320
Physical Location & Seq
Reverse (-)
23276858 .. 23277965
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012864

Sequence Viewer

Length: 546 bp
ATGGCGGACCAGGAGGTTCAGAACGGTGGAGCTGAGAAGGTCGCTACTGAGGCCAAGAGTGTGGTTCTCGATCTCCACGCTGTGCTGGGAGTCGAAGCACCCAAGGCTGACGACGCCGTTCAGTTCTACAAGGCTGTCTTCGGCGCTGTGGAGGTTGAGCGTGGCGCGAACGCCAAGCGTAAGGCGGACCTGGAGCGACCTCTCATCCATGTGTTGCTTCAGTTTGGCTCCTGCAAGGTTTTGGTCGTTGATCGCCTTGAGGATGAGTTGAAGGCTCATCAAAACAGCACTGGTCTCGCATTGGTCTGTGAGGGGGATATTGAAGCTGTGATTAAGAAGGTTGTGGCTGCTGGAGGGATTACAGAGAGTGGTGGTGTGATTGAGGACCATGCCTTCCCTGGAAAGCAATACTCCGGCGAAGTCAAGGACCCATTTGGCATTGTCTGGACCATTGTTGGCAACAAGACCATCACCGCTGCTTCTGAGACTGGGCCAGAAAACACCACTGCTGCCGAGATTGAGGCAAACAACACCACTGATGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3-dmu-9_3-mt Glyoxalase

Protein Analysis

182

Amino Acids

19.03

Weight (kDa)

4.73

Isoelectric Point (pI)

28.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g48480-like_N PF22656 29 - 84 3e-19 Glyoxalase At5g48480-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48480
fragaria_vesca FvH4_2g17810
malus_domestica MD05G1159600.v1.1 MD10G1152100.v1.1
prunus_persica Prupe.8G190400_v2.0.a1
pyrus_communis pycom05g15120 pycom10g13110
rosa_chinensis RchiOBHm_Chr6g0282841
rosa_laevigata RLG00000012864 RLG00000021743
rosa_multiflora Rmu_sc0006070.1_g000011
rosa_roxburghii Rroxscaffold_7G00185960
rosa_rugosa Rorug02G0530700 Rorug06G0149900
rosa_samantha Rh2BG610300 Rh6AG263200 Rh6BG266100 Rh6CG265300 Rh6DG257900
rosa_wichuraiana Rw6G022720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 167
AciI CCGC 3 cut(s) 5, 185, 474
AcuI CTGAAG 1 cut(s) 203
AcyI GRCGYC 1 cut(s) 114
AdeI CACNNNGTG 1 cut(s) 82
AgsI TTSAA 2 cut(s) 271, 323
AjnI CCWGG 3 cut(s) 9, 189, 397
AluBI AGCT 2 cut(s) 32, 326
AluI AGCT 2 cut(s) 32, 326
Alw26I GTCTC 2 cut(s) 299, 479
AoxI GGCC 2 cut(s) 51, 491
ApeKI GCWGC 3 cut(s) 347, 476, 509
AspLEI GCGC 2 cut(s) 146, 167
AspS9I GGNCC 6 cut(s) 7, 187, 385, 427, 447, 491
AsuHPI GGTGA 1 cut(s) 463
AvaII GGWCC 5 cut(s) 7, 187, 385, 427, 447
BbsI GAAGAC 1 cut(s) 130
BbvI GCAGC 3 cut(s) 334, 463, 496
BccI CCATC 1 cut(s) 476
BceAI ACGGC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BciT130I CCWGG 3 cut(s) 11, 191, 399
BcoDI GTCTC 2 cut(s) 299, 479
BfoI RGCGCY 1 cut(s) 147
BisI GCNGC 3 cut(s) 348, 477, 510
BlsI GCNGC 3 cut(s) 349, 478, 511
Bme1390I CCNGG 3 cut(s) 11, 191, 399
Bme18I GGWCC 5 cut(s) 7, 187, 385, 427, 447
BmgT120I GGNCC 6 cut(s) 7, 187, 385, 427, 447, 491
BmiI GGNNCC 2 cut(s) 229, 429
BmrFI CCNGG 3 cut(s) 11, 191, 399
BmrI ACTGGG 1 cut(s) 498
BmsI GCATC 1 cut(s) 529
BmuI ACTGGG 1 cut(s) 498
BpiI GAAGAC 1 cut(s) 130
BpmI CTGGAG 2 cut(s) 212, 372
BpuEI CTTGAG 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 2 cut(s) 102, 397
Bse1I ACTGG 2 cut(s) 295, 493
BseBI CCWGG 3 cut(s) 11, 191, 399
BseDI CCNNGG 2 cut(s) 102, 397
BseGI GGATG 2 cut(s) 204, 268
BseMII CTCAG 3 cut(s) 24, 39, 474
BseNI ACTGG 2 cut(s) 295, 493
BseXI GCAGC 3 cut(s) 334, 463, 496
BseYI CCCAGC 1 cut(s) 85
Bsh1236I CGCG 1 cut(s) 167
BshFI GGCC 2 cut(s) 53, 493
BsiSI CCGG 1 cut(s) 414
BsmAI GTCTC 2 cut(s) 299, 479
BsnI GGCC 2 cut(s) 53, 493
Bso31I GGTCTC 1 cut(s) 299
Bsp143I GATC 2 cut(s) 70, 250
BspACI CCGC 3 cut(s) 5, 185, 474
BspANI GGCC 2 cut(s) 53, 493
BspCNI CTCAG 3 cut(s) 25, 40, 475
BspFNI CGCG 1 cut(s) 167
BspLI GGNNCC 2 cut(s) 229, 429
BspTNI GGTCTC 1 cut(s) 299
BsrI ACTGG 2 cut(s) 295, 493
BssECI CCNNGG 2 cut(s) 102, 397
BssMI GATC 2 cut(s) 70, 250
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 3 cut(s) 11, 191, 399
Bst4CI ACNGT 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 114
BstDEI CTNAG 3 cut(s) 33, 48, 483
BstF5I GGATG 2 cut(s) 204, 268
BstFNI CGCG 1 cut(s) 167
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 2 cut(s) 146, 167
BstKTI GATC 2 cut(s) 73, 253
BstMAI GTCTC 2 cut(s) 299, 479
BstMBI GATC 2 cut(s) 70, 250
BstMWI GCNNNNNNNGC 3 cut(s) 50, 104, 113
BstNI CCWGG 3 cut(s) 11, 191, 399
BstSCI CCNGG 3 cut(s) 9, 189, 397
BstUI CGCG 1 cut(s) 167
BstV1I GCAGC 3 cut(s) 334, 463, 496
BstV2I GAAGAC 1 cut(s) 130
BstXI CCANNNNNNTGG 1 cut(s) 61
BsuRI GGCC 2 cut(s) 53, 493
BtsCI GGATG 2 cut(s) 204, 268
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 3 cut(s) 288, 504, 534
CfoI GCGC 2 cut(s) 146, 167
Cfr13I GGNCC 6 cut(s) 7, 187, 385, 427, 447, 491
CseI GACGC 1 cut(s) 122
CviAII CATG 2 cut(s) 209, 389
CviJI RGCY 9 cut(s) 32, 53, 107, 134, 228, 275, 326, 347, 493
CviKI_1 RGCY 9 cut(s) 32, 53, 107, 134, 228, 275, 326, 347, 493
DdeI CTNAG 3 cut(s) 33, 48, 483
DpnI GATC 2 cut(s) 72, 252
DpnII GATC 2 cut(s) 70, 250
DraIII CACNNNGTG 1 cut(s) 82
EciI GGCGGA 2 cut(s) 20, 200
Eco130I CCWWGG 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 5 cut(s) 7, 187, 385, 427, 447
Eco57I CTGAAG 1 cut(s) 203
EcoO109I RGGNCCY 1 cut(s) 427
EcoRII CCWGG 3 cut(s) 9, 189, 397
EcoT14I CCWWGG 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 2 cut(s) 212, 392
FaiI YATR 2 cut(s) 210, 390
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FatI CATG 2 cut(s) 208, 388
Fnu4HI GCNGC 3 cut(s) 348, 477, 510
FokI GGATG 2 cut(s) 191, 275
Fsp4HI GCNGC 3 cut(s) 348, 477, 510
GlaI GCGC 2 cut(s) 145, 166
GluI GCNGC 3 cut(s) 348, 477, 510
GsaI CCCAGC 1 cut(s) 89
GsuI CTGGAG 2 cut(s) 212, 372
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 2 cut(s) 53, 493
HapII CCGG 1 cut(s) 414
HgaI GACGC 1 cut(s) 122
HhaI GCGC 2 cut(s) 146, 167
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 2 cut(s) 212, 392
Hin6I GCGC 2 cut(s) 144, 165
HinP1I GCGC 2 cut(s) 144, 165
HinfI GANTC 1 cut(s) 90
HpaII CCGG 1 cut(s) 414
HphI GGTGA 1 cut(s) 463
Hpy188I TCNGA 2 cut(s) 21, 484
Hpy188III TCNNGA 2 cut(s) 68, 445
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 4 cut(s) 31, 265, 331, 403
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4V TGCA 1 cut(s) 234
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 104, 113
HpyF3I CTNAG 3 cut(s) 33, 48, 483
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 2 cut(s) 212, 392
HspAI GCGC 2 cut(s) 144, 165
Kzo9I GATC 2 cut(s) 70, 250
LmnI GCTCC 3 cut(s) 29, 193, 233
Lsp1109I GCAGC 3 cut(s) 334, 463, 496
LweI GCATC 1 cut(s) 529
MalI GATC 2 cut(s) 72, 252
MboI GATC 2 cut(s) 70, 250
MboII GAAGA 1 cut(s) 130
MlyI GAGTC 1 cut(s) 99
MnlI CCTC 9 cut(s) 7, 43, 145, 210, 253, 304, 347, 376, 514
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 1 cut(s) 414
MspR9I CCNGG 3 cut(s) 11, 191, 399
MvaI CCWGG 3 cut(s) 11, 191, 399
MvnI CGCG 1 cut(s) 167
MwoI GCNNNNNNNGC 3 cut(s) 50, 104, 113
NdeII GATC 2 cut(s) 70, 250
NlaIII CATG 2 cut(s) 212, 392
NlaIV GGNNCC 2 cut(s) 229, 429
NmeAIII GCCGAG 1 cut(s) 538
PkrI GCNGC 3 cut(s) 349, 478, 511
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PpuMI RGGWCCY 1 cut(s) 427
Psp5II RGGWCCY 1 cut(s) 427
Psp6I CCWGG 3 cut(s) 9, 189, 397
PspFI CCCAGC 1 cut(s) 85
PspGI CCWGG 3 cut(s) 9, 189, 397
PspN4I GGNNCC 2 cut(s) 229, 429
PspPI GGNCC 6 cut(s) 7, 187, 385, 427, 447, 491
PspPPI RGGWCCY 1 cut(s) 427
RseI CAYNNNNRTG 1 cut(s) 209
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 3 cut(s) 348, 477, 510
Sau3AI GATC 2 cut(s) 70, 250
Sau96I GGNCC 6 cut(s) 7, 187, 385, 427, 447, 491
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 3 cut(s) 11, 191, 399
SetI ASST 9 cut(s) 18, 34, 42, 156, 192, 202, 240, 328, 342
SfaNI GCATC 1 cut(s) 529
SinI GGWCC 5 cut(s) 7, 187, 385, 427, 447
SmiMI CAYNNNNRTG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SsiI CCGC 3 cut(s) 5, 185, 474
StyD4I CCNGG 3 cut(s) 9, 189, 397
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 26
TaqI TCGA 2 cut(s) 69, 93
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 3 cut(s) 295, 511, 541
TseI GCWGC 3 cut(s) 347, 476, 509
TspRI CASTG 3 cut(s) 295, 511, 541
VpaK11BI GGWCC 5 cut(s) 7, 187, 385, 427, 447
XcmI CCANNNNNNNNNTGG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.