MD10G1152100.v1.1

At5g48480

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011320
Physical Location & Seq
Forward (+)
23903180 .. 23904426
1247 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1152100.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGAGGAGCAAAAGGTACAGAAAGGCGTGGCCGCCGAGAAGGTCGCACCCGTGTCCCCCATCTCTGTCACGGAGTGCTACCCTCGTCTCTCGATCAAGGCACCCAAGGGCGAAGACGCCATCAAGTTCTACGAGACCGTCTTCGGCGCCGTGGAGCTTGAGCGTGAGAGTGATCTCAAGCGCAAGGCTGACCAGGAGCGTCCCTTCATCTACAATGCTAAGCTCAAGCTTGGGTCCTTCACCTTCCTTGTGACTGAGTCTGACTCGATTGTTGACTCCGGTGACGCCGCAGCTCTGGCTGACGGTGGTTTTAAGCATCTGTTGCTTACTGTTGGGGACGTCGATGCGGCTGTGTCCAAGGCGGTCCAGGCGGGAGCTGCGGCGGTTGGGGAGATCAAAGCTGGTGGTGCTTTCTTTGGCGACGAGCAGCGTTGGGCTCAGATTAAGGATCCTTTCGGCATTAACTGGTTCTTCGTCACCCCCCTTGAGAAGAAGGCGTCTGTGGCCGAAGCCCCGGTTACCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3-dmu-9_3-mt Glyoxalase

Protein Analysis

176

Amino Acids

18.77

Weight (kDa)

5.04

Isoelectric Point (pI)

29.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g48480-like_N PF22656 30 - 85 3.5e-20 Glyoxalase At5g48480-like, N-terminal domain
Glyoxalase PF00903 38 - 156 1.2e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48480
fragaria_vesca FvH4_2g17810
malus_domestica MD05G1159600.v1.1 MD10G1152100.v1.1
prunus_persica Prupe.8G190400_v2.0.a1
pyrus_communis pycom05g15120 pycom10g13110
rosa_chinensis RchiOBHm_Chr6g0282841
rosa_laevigata RLG00000012864 RLG00000021743
rosa_multiflora Rmu_sc0006070.1_g000011
rosa_roxburghii Rroxscaffold_7G00185960
rosa_rugosa Rorug02G0530700 Rorug06G0149900
rosa_samantha Rh2BG610300 Rh6AG263200 Rh6BG266100 Rh6CG265300 Rh6DG257900
rosa_wichuraiana Rw6G022720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 342
AccB1I GGYRCC 2 cut(s) 100, 146
AciI CCGC 7 cut(s) 33, 288, 347, 362, 371, 380, 383
AclWI GGATC 2 cut(s) 443, 456
AcoI YGGCCR 2 cut(s) 30, 504
AcyI GRCGYC 5 cut(s) 117, 147, 285, 339, 497
AdeI CACNNNGTG 1 cut(s) 75
AfaI GTAC 1 cut(s) 18
AjnI CCWGG 2 cut(s) 192, 366
AluBI AGCT 6 cut(s) 157, 223, 229, 293, 377, 401
AluI AGCT 6 cut(s) 157, 223, 229, 293, 377, 401
Alw26I GTCTC 2 cut(s) 92, 128
AlwI GGATC 2 cut(s) 443, 456
AoxI GGCC 2 cut(s) 30, 504
ApeKI GCWGC 3 cut(s) 290, 377, 427
AspLEI GCGC 2 cut(s) 149, 183
AspS9I GGNCC 2 cut(s) 234, 364
AsuC2I CCSGG 1 cut(s) 515
AsuHPI GGTGA 3 cut(s) 232, 293, 469
AvaII GGWCC 2 cut(s) 234, 364
BamHI GGATCC 1 cut(s) 448
BanI GGYRCC 2 cut(s) 100, 146
BanII GRGCYC 1 cut(s) 439
BbsI GAAGAC 2 cut(s) 120, 133
BbvI GCAGC 3 cut(s) 302, 364, 439
BccI CCATC 2 cut(s) 68, 128
BceAI ACGGC 1 cut(s) 134
BciT130I CCWGG 2 cut(s) 194, 368
BcnI CCSGG 1 cut(s) 515
BcoDI GTCTC 2 cut(s) 92, 128
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 7 cut(s) 33, 288, 291, 348, 378, 381, 428
BlpI GCTNAGC 1 cut(s) 219
BlsI GCNGC 7 cut(s) 34, 289, 292, 349, 379, 382, 429
Bme1390I CCNGG 3 cut(s) 194, 368, 515
Bme18I GGWCC 2 cut(s) 234, 364
BmgT120I GGNCC 2 cut(s) 234, 364
BmiI GGNNCC 4 cut(s) 102, 148, 235, 450
BmrFI CCNGG 3 cut(s) 194, 368, 515
BmsI GCATC 2 cut(s) 325, 334
BpiI GAAGAC 2 cut(s) 120, 133
BplI GAGNNNNNCTC 2 cut(s) 248, 280
Bpu1102I GCTNAGC 1 cut(s) 219
BpuEI CTTGAG 4 cut(s) 161, 179, 209, 506
BpuMI CCSGG 1 cut(s) 515
BsaHI GRCGYC 5 cut(s) 117, 147, 285, 339, 497
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 4 cut(s) 105, 150, 357, 513
BsaWI WCCGGW 1 cut(s) 278
Bse1I ACTGG 1 cut(s) 470
BseBI CCWGG 2 cut(s) 194, 368
BseDI CCNNGG 4 cut(s) 105, 150, 357, 513
BseMII CTCAG 2 cut(s) 246, 452
BseNI ACTGG 1 cut(s) 470
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 3 cut(s) 302, 364, 439
BshFI GGCC 2 cut(s) 32, 506
BshNI GGYRCC 2 cut(s) 100, 146
BsiSI CCGG 2 cut(s) 279, 515
BslFI GGGAC 3 cut(s) 40, 186, 350
BsmAI GTCTC 2 cut(s) 92, 128
BsmBI CGTCTC 1 cut(s) 92
BsmFI GGGAC 3 cut(s) 40, 186, 350
BsnI GGCC 2 cut(s) 32, 506
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 4 cut(s) 93, 172, 393, 448
Bsp1720I GCTNAGC 1 cut(s) 219
BspACI CCGC 7 cut(s) 33, 288, 347, 362, 371, 380, 383
BspANI GGCC 2 cut(s) 32, 506
BspCNI CTCAG 2 cut(s) 247, 451
BspLI GGNNCC 4 cut(s) 102, 148, 235, 450
BspPI GGATC 2 cut(s) 443, 456
BspT107I GGYRCC 2 cut(s) 100, 146
BspTNI GGTCTC 1 cut(s) 128
BsrI ACTGG 1 cut(s) 470
BssECI CCNNGG 4 cut(s) 105, 150, 357, 513
BssMI GATC 4 cut(s) 93, 172, 393, 448
BssNI GRCGYC 5 cut(s) 117, 147, 285, 339, 497
BssT1I CCWWGG 2 cut(s) 105, 357
Bst2UI CCWGG 2 cut(s) 194, 368
Bst4CI ACNGT 4 cut(s) 139, 305, 331, 523
BstACI GRCGYC 5 cut(s) 117, 147, 285, 339, 497
BstAPI GCANNNNNTGC 1 cut(s) 322
BstDEI CTNAG 3 cut(s) 219, 255, 438
BstDSI CCRYGG 1 cut(s) 150
BstEII GGTNACC 1 cut(s) 517
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 2 cut(s) 149, 183
BstKTI GATC 4 cut(s) 96, 175, 396, 451
BstMAI GTCTC 2 cut(s) 92, 128
BstMBI GATC 4 cut(s) 93, 172, 393, 448
BstMWI GCNNNNNNNGC 6 cut(s) 296, 322, 368, 377, 407, 503
BstNI CCWGG 2 cut(s) 194, 368
BstPI GGTNACC 1 cut(s) 517
BstSCI CCNGG 3 cut(s) 192, 366, 513
BstV1I GCAGC 3 cut(s) 302, 364, 439
BstV2I GAAGAC 2 cut(s) 120, 133
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuRI GGCC 2 cut(s) 32, 506
BtgI CCRYGG 1 cut(s) 150
CfoI GCGC 2 cut(s) 149, 183
Cfr13I GGNCC 2 cut(s) 234, 364
CseI GACGC 4 cut(s) 125, 188, 293, 486
Csp6I GTAC 1 cut(s) 17
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviQI GTAC 1 cut(s) 17
DdeI CTNAG 3 cut(s) 219, 255, 438
DinI GGCGCC 1 cut(s) 148
DpnI GATC 4 cut(s) 95, 174, 395, 450
DpnII GATC 4 cut(s) 93, 172, 393, 448
DraIII CACNNNGTG 1 cut(s) 75
EaeI YGGCCR 2 cut(s) 30, 504
Eco130I CCWWGG 2 cut(s) 105, 357
Eco24I GRGCYC 1 cut(s) 439
Eco31I GGTCTC 1 cut(s) 128
Eco47I GGWCC 2 cut(s) 234, 364
Eco91I GGTNACC 1 cut(s) 517
EcoO109I RGGNCCY 1 cut(s) 234
EcoO65I GGTNACC 1 cut(s) 517
EcoRII CCWGG 2 cut(s) 192, 366
EcoT14I CCWWGG 2 cut(s) 105, 357
EcoT38I GRGCYC 1 cut(s) 439
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 2 cut(s) 105, 357
Esp3I CGTCTC 1 cut(s) 92
FaqI GGGAC 3 cut(s) 40, 186, 350
FauI CCCGC 1 cut(s) 364
Fnu4HI GCNGC 7 cut(s) 33, 288, 291, 348, 378, 381, 428
FriOI GRGCYC 1 cut(s) 439
Fsp4HI GCNGC 7 cut(s) 33, 288, 291, 348, 378, 381, 428
GlaI GCGC 2 cut(s) 148, 182
GluI GCNGC 7 cut(s) 33, 288, 291, 348, 378, 381, 428
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 2 cut(s) 32, 506
HapII CCGG 2 cut(s) 279, 515
HgaI GACGC 4 cut(s) 125, 188, 293, 486
HhaI GCGC 2 cut(s) 149, 183
Hin1I GRCGYC 5 cut(s) 117, 147, 285, 339, 497
Hin6I GCGC 2 cut(s) 147, 181
HinP1I GCGC 2 cut(s) 147, 181
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 3 cut(s) 257, 263, 275
HpaII CCGG 2 cut(s) 279, 515
HphI GGTGA 3 cut(s) 232, 293, 469
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 3 cut(s) 262, 441, 527
Hpy188III TCNNGA 1 cut(s) 91
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 2 cut(s) 344, 425
HpyAV CCTTC 5 cut(s) 34, 214, 247, 253, 487
HpyCH4III ACNGT 4 cut(s) 139, 305, 331, 523
HpyCH4IV ACGT 1 cut(s) 339
HpyF10VI GCNNNNNNNGC 6 cut(s) 296, 322, 368, 377, 407, 503
HpyF3I CTNAG 3 cut(s) 219, 255, 438
HpySE526I ACGT 1 cut(s) 339
Hsp92I GRCGYC 5 cut(s) 117, 147, 285, 339, 497
HspAI GCGC 2 cut(s) 147, 181
KasI GGCGCC 1 cut(s) 146
Kzo9I GATC 4 cut(s) 93, 172, 393, 448
LmnI GCTCC 4 cut(s) 7, 154, 196, 374
LpnPI CCDG 8 cut(s) 179, 206, 281, 292, 353, 380, 387, 451
Lsp1109I GCAGC 3 cut(s) 302, 364, 439
LweI GCATC 2 cut(s) 325, 334
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 5 cut(s) 67, 250, 281, 475, 517
MalI GATC 4 cut(s) 95, 174, 395, 450
MboI GATC 4 cut(s) 93, 172, 393, 448
MboII GAAGA 4 cut(s) 125, 133, 463, 502
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 1 cut(s) 439
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 3 cut(s) 257, 266, 269
MnlI CCTC 1 cut(s) 93
MseI TTAA 3 cut(s) 312, 444, 462
MspI CCGG 2 cut(s) 279, 515
MspR9I CCNGG 3 cut(s) 194, 368, 515
MvaI CCWGG 2 cut(s) 194, 368
MwoI GCNNNNNNNGC 6 cut(s) 296, 322, 368, 377, 407, 503
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 1 cut(s) 515
NdeII GATC 4 cut(s) 93, 172, 393, 448
NlaIV GGNNCC 4 cut(s) 102, 148, 235, 450
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 4 cut(s) 67, 250, 281, 475
PflFI GACNNNGTC 1 cut(s) 256
PkrI GCNGC 7 cut(s) 34, 289, 292, 349, 379, 382, 429
PleI GAGTC 3 cut(s) 257, 265, 269
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 3 cut(s) 257, 265, 269
PpuMI RGGWCCY 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 234
Psp6I CCWGG 2 cut(s) 192, 366
PspEI GGTNACC 1 cut(s) 517
PspGI CCWGG 2 cut(s) 192, 366
PspN4I GGNNCC 4 cut(s) 102, 148, 235, 450
PspPI GGNCC 2 cut(s) 234, 364
PspPPI RGGWCCY 1 cut(s) 234
PsuI RGATCY 1 cut(s) 448
PsyI GACNNNGTC 1 cut(s) 256
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
SaqAI TTAA 3 cut(s) 312, 444, 462
SatI GCNGC 7 cut(s) 33, 288, 291, 348, 378, 381, 428
Sau3AI GATC 4 cut(s) 93, 172, 393, 448
Sau96I GGNCC 2 cut(s) 234, 364
SchI GAGTC 3 cut(s) 257, 266, 269
ScrFI CCNGG 3 cut(s) 194, 368, 515
SduI GDGCHC 1 cut(s) 439
SfaNI GCATC 2 cut(s) 325, 334
SfoI GGCGCC 1 cut(s) 148
SinI GGWCC 2 cut(s) 234, 364
SmlI CTYRAG 4 cut(s) 158, 176, 224, 485
SmoI CTYRAG 4 cut(s) 158, 176, 224, 485
SsiI CCGC 7 cut(s) 33, 288, 347, 362, 371, 380, 383
SspDI GGCGCC 1 cut(s) 146
StyD4I CCNGG 3 cut(s) 192, 366, 513
StyI CCWWGG 2 cut(s) 105, 357
TaaI ACNGT 4 cut(s) 139, 305, 331, 523
TaiI ACGT 1 cut(s) 342
TaqI TCGA 3 cut(s) 92, 266, 342
TauI GCSGC 4 cut(s) 35, 290, 350, 383
Tru1I TTAA 3 cut(s) 312, 444, 462
Tru9I TTAA 3 cut(s) 312, 444, 462
TseFI GTSAC 4 cut(s) 67, 250, 281, 475
TseI GCWGC 3 cut(s) 290, 377, 427
Tsp45I GTSAC 4 cut(s) 67, 250, 281, 475
TspDTI ATGAA 1 cut(s) 196
TspGWI ACGGA 1 cut(s) 86
Tth111I GACNNNGTC 1 cut(s) 256
VpaK11BI GGWCC 2 cut(s) 234, 364
ZraI GACGTC 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.