Rh2BG610300

At5g48480

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0011320
Physical Location & Seq
Forward (+)
83122216 .. 83122926
711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG610300.1

Sequence Viewer

Length: 399 bp
ATGGCGAACATCATCAGCGTTTCTTCAATGGCGGTTAATATCAGTGTTTCGTCAATGGACGAGGATGAAAGGAGCTCTCGGTTGTCGCCCATGGAGGCGTGGGCAGTGCTCGGAGTGGAAGCTCTAAAGGCTGGGCTCACCGTCGACTTCTACAAGAACGTGTTTGGAGCCATCGAGACTTCCCGTTTCACATTCCGCCACCGTGGAAAGAACCATCTTCTTCTATGTGTTGTGCTGCTGTTTGGCACAACGAAGATAACCATCTTCGATGAGATCGATGGTGGTGATGGTTCCAAGCATGGTGGTATCAGATTGATGTCTGATCAAGATTTGAAAGAAGTTATTGCTAAGGTGGAGCGTGCGGGAGCTGTATCTGAAGGAGATTTCAGTGAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3-dmu-9_3-mt Glyoxalase

Protein Analysis

132

Amino Acids

14.34

Weight (kDa)

5.14

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g48480-like_N PF22656 37 - 88 5.4e-07 Glyoxalase At5g48480-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48480
fragaria_vesca FvH4_2g17810
malus_domestica MD05G1159600.v1.1 MD10G1152100.v1.1
prunus_persica Prupe.8G190400_v2.0.a1
pyrus_communis pycom05g15120 pycom10g13110
rosa_chinensis RchiOBHm_Chr6g0282841
rosa_laevigata RLG00000012864 RLG00000021743
rosa_multiflora Rmu_sc0006070.1_g000011
rosa_roxburghii Rroxscaffold_7G00185960
rosa_rugosa Rorug02G0530700 Rorug06G0149900
rosa_samantha Rh2BG610300 Rh6AG263200 Rh6BG266100 Rh6CG265300 Rh6DG257900
rosa_wichuraiana Rw6G022720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 144
AciI CCGC 3 cut(s) 32, 196, 362
AcuI CTGAAG 1 cut(s) 396
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 2 cut(s) 27, 334
AluBI AGCT 3 cut(s) 75, 122, 368
AluI AGCT 3 cut(s) 75, 122, 368
Alw21I GWGCWC 2 cut(s) 77, 111
Alw26I GTCTC 1 cut(s) 170
ApeKI GCWGC 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 130, 296
BanII GRGCYC 2 cut(s) 77, 138
Bbv12I GWGCWC 2 cut(s) 77, 111
BbvI GCAGC 1 cut(s) 222
BccI CCATC 5 cut(s) 179, 222, 269, 272, 281
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 170
BisI GCNGC 1 cut(s) 236
BlsI GCNGC 1 cut(s) 237
BmiI GGNNCC 2 cut(s) 169, 292
Bpu10I CCTNAGC 1 cut(s) 348
Bsa29I ATCGAT 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 260
BsaJI CCNNGG 2 cut(s) 90, 202
Bse8I GATNNNNATC 1 cut(s) 260
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 2 cut(s) 90, 202
BseGI GGATG 1 cut(s) 70
BseJI GATNNNNATC 1 cut(s) 260
BseXI GCAGC 1 cut(s) 222
BseYI CCCAGC 1 cut(s) 131
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 2 cut(s) 77, 111
BsmAI GTCTC 1 cut(s) 170
Bsp1286I GDGCHC 3 cut(s) 77, 111, 138
Bsp143I GATC 2 cut(s) 273, 322
Bsp19I CCATGG 1 cut(s) 90
BspACI CCGC 3 cut(s) 32, 196, 362
BspDI ATCGAT 1 cut(s) 276
BspLI GGNNCC 2 cut(s) 169, 292
BssECI CCNNGG 2 cut(s) 90, 202
BssMI GATC 2 cut(s) 273, 322
BssT1I CCWWGG 1 cut(s) 90
Bst4CI ACNGT 2 cut(s) 142, 203
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 1 cut(s) 348
BstDSI CCRYGG 2 cut(s) 90, 202
BstF5I GGATG 1 cut(s) 70
BstKTI GATC 2 cut(s) 276, 325
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 2 cut(s) 273, 322
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstV1I GCAGC 1 cut(s) 222
Bsu15I ATCGAT 1 cut(s) 276
BsuTUI ATCGAT 1 cut(s) 276
BtgI CCRYGG 2 cut(s) 90, 202
BtsCI GGATG 1 cut(s) 70
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 3 cut(s) 49, 111, 394
Cac8I GCNNGC 1 cut(s) 360
ClaI ATCGAT 1 cut(s) 276
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviAII CATG 2 cut(s) 91, 299
CviJI RGCY 6 cut(s) 75, 122, 131, 136, 170, 368
CviKI_1 RGCY 6 cut(s) 75, 122, 131, 136, 170, 368
DdeI CTNAG 1 cut(s) 348
DpnI GATC 2 cut(s) 275, 324
DpnII GATC 2 cut(s) 273, 322
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 75
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 2 cut(s) 77, 138
Eco53kI GAGCTC 1 cut(s) 75
Eco57I CTGAAG 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 2 cut(s) 77, 138
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 2 cut(s) 94, 302
FaiI YATR 3 cut(s) 92, 226, 300
FatI CATG 2 cut(s) 90, 298
FauI CCCGC 1 cut(s) 355
FbaI TGATCA 1 cut(s) 322
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 1 cut(s) 236
FokI GGATG 1 cut(s) 77
FriOI GRGCYC 2 cut(s) 77, 138
Fsp4HI GCNGC 1 cut(s) 236
GluI GCNGC 1 cut(s) 236
GsaI CCCAGC 1 cut(s) 135
Hin1II CATG 2 cut(s) 94, 302
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HphI GGTGA 2 cut(s) 130, 296
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 4 cut(s) 113, 311, 322, 376
Hpy188III TCNNGA 2 cut(s) 175, 326
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 371
HpyCH4III ACNGT 2 cut(s) 142, 203
HpyCH4IV ACGT 1 cut(s) 159
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 1 cut(s) 348
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 2 cut(s) 94, 302
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 2 cut(s) 273, 322
LmnI GCTCC 4 cut(s) 72, 167, 355, 365
LpnPI CCDG 1 cut(s) 117
Lsp1109I GCAGC 1 cut(s) 222
MaeII ACGT 1 cut(s) 159
MalI GATC 2 cut(s) 275, 324
MboI GATC 2 cut(s) 273, 322
MboII GAAGA 5 cut(s) 15, 209, 212, 256, 265
MhlI GDGCHC 3 cut(s) 77, 111, 138
MnlI CCTC 2 cut(s) 55, 88
MseI TTAA 1 cut(s) 36
MwoI GCNNNNNNNGC 1 cut(s) 128
NcoI CCATGG 1 cut(s) 90
NdeII GATC 2 cut(s) 273, 322
NlaIII CATG 2 cut(s) 94, 302
NlaIV GGNNCC 2 cut(s) 169, 292
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PkrI GCNGC 1 cut(s) 237
Psp124BI GAGCTC 1 cut(s) 77
PspFI CCCAGC 1 cut(s) 131
PspN4I GGNNCC 2 cut(s) 169, 292
SacI GAGCTC 1 cut(s) 77
SalI GTCGAC 1 cut(s) 143
SaqAI TTAA 1 cut(s) 36
SatI GCNGC 1 cut(s) 236
Sau3AI GATC 2 cut(s) 273, 322
SduI GDGCHC 3 cut(s) 77, 111, 138
SetI ASST 5 cut(s) 77, 124, 162, 354, 370
SsiI CCGC 3 cut(s) 32, 196, 362
SstI GAGCTC 1 cut(s) 77
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 2 cut(s) 142, 203
TaiI ACGT 1 cut(s) 162
TaqI TCGA 4 cut(s) 144, 174, 267, 276
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TscAI CASTG 3 cut(s) 49, 111, 394
TseI GCWGC 1 cut(s) 235
TspDTI ATGAA 1 cut(s) 81
TspRI CASTG 3 cut(s) 49, 111, 394
XmiI GTMKAC 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.