AT5G48880

3-keto-acyl-CoA thiolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009799
Physical Location & Seq
Reverse (-)
19814395 .. 19817955
3561 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G48880.3

Sequence Viewer

Length: 1245 bp
ATGGCTGCTTTTGGAGATGACATTGTGATTGTAGCGGCATATCGTACCGCCATTTGCAAAGCGAGACGTGGAGGTTTCAAAGACACTCTTCCTGATGATCTTCTTGCTTCTGTTCTTAAGGCTGTAGTGGAAAGAACATCTTTGGATCCAAGTGAAGTTGGTGATATCGTTGTTGGTACCGTGATAGCGCCTGGTTCTCAGAGAGCAATGGAGTGTAGAGTTGCAGCTTATTTTGCTGGTTTTCCTGACTCCGTGCCAGTTAGAACTGTCAATAGACAATGCTCATCAGGACTACAAGCAGTTGCTGATGTTGCTGCTTCCATTAGAGCTGGTTATTACGACATTGGTATTGGTGCTGGAGTGGAATCAATGTCAACTGATCATATTCCTGGAGGCGGCTTTCATGGCTCTAATCCAAGAGCACAGGATTTCCCAAAAGCCCGTGATTGTTTGCTTCCAATGGGAATTACTTCTGAAAACGTTGCAGAAAGGTTCGGTGTCACAAGAGAAGAGCAAGATATGGCTGCGGTGGAGTCTCACAAACGCGCTGCAGCTGCAATCGCGTCTGGTAAACTCAAGGATGAAATCATTCCTGTTGCTACTAAGATTGTGGACCCTGAGACTAAAGCAGAGAAGGCAATCGTCGTATCTGTTGATGACGGTGTACGTCCAAACTCAAACATGGCAGATTTGGCAAAGCTGAAGACTGTCTTTAAACAGAACGGTTCCACCACAGCTGGCAATGCTAGTCAGATCAGTGATGGTGCTGGAGCAGTACTGCTAATGAAGAGAAGTTTGGCTATGAAGAAGGGACTTCCCATTCTTGGAGTATTCAGGAGTTTTGCTGTTACTGGTGTGGAACCATCTGTAATGGGTATTGGTCCAGCTGTTGCCATTCCCGCTGCAACTAAGCTCGCAGGGCTCAACGTCAGCGATATTGATCTATTCGAGATCAATGAGGCATTTGCATCTCAGTATGTGTACTCTTGCAAGAAGTTAGAGCTGGATATGGAAAAGGTCAATGTTAATGGAGGAGCCATTGCTATTGGCCATCCCCTGGGTGCTACAGGAGCTCGATGTGTTGCGACATTGTTGCACGAGATGAAGCGGAGAGGAAAAGATTGCCGCTTTGGAGTAATCTCAATGTGCATAGGCACTGGAATGGGAGCTGCAGCTGTTTTTGAGAGGGGAGACTCTGTTGATAACTTGTCCAACGCTCGTGTGGCTAACGGGGATAGTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003988 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009507 GO:0009514 GO:0009536 GO:0009611 GO:0009657 GO:0009694 GO:0009695 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010111 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016042 GO:0016043 GO:0016053 GO:0016054 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019395 GO:0019752 GO:0023051 GO:0023056 GO:0030258 GO:0031090 GO:0031974 GO:0031981 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046395 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072330 GO:0098588 GO:0098805 GO:1901419 GO:1901421 GO:1901575 GO:1901576 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko00071 ko00280 ko00592 ko01040 ko01100 ko01110 ko01130 ko01212 ko03320 ko04146 map00071 map00280 map00592 map01040 map01100 map01110 map01130 map01212 map03320 map04146
Pfam Domains
Protein Families
Thiolase_C Thiolase_N

Protein Analysis

414

Amino Acids

43.17

Weight (kDa)

6.61

Isoelectric Point (pI)

28.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thiolase_N PF00108 51 - 308 3.1e-72 Thiolase, N-terminal domain
Thiolase_C PF02803 316 - 439 2.9e-48 Thiolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009799)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48880 AT5G48880 AT5G48880 AT5G48880
fragaria_vesca FvH4_2g19500
malus_domestica MD05G1179100.v1.1 MD06G1074100.v1.1 MD10G1167800.v1.1
prunus_persica Prupe.8G206400_v2.0.a1
pyrus_communis pycom05g16560
rosa_chinensis RchiOBHm_Chr6g0285181
rosa_laevigata RLG00000012663
rosa_multiflora Rmu_sc0005710.1_g000006 Rmu_sc0006801.1_g000004
rosa_roxburghii Rroxscaffold_7G00183440
rosa_rugosa Rorug06G0173100
rosa_samantha Rh6AG283100 Rh6CG285800 Rh6DG278800
rosa_wichuraiana Rw0G000370 Rw6G024390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 176
AccB1I GGYRCC 1 cut(s) 176
AccB7I CCANNNNNTGG 1 cut(s) 1057
AccII CGCG 2 cut(s) 546, 563
AciI CCGC 7 cut(s) 35, 48, 396, 527, 900, 1108, 1126
AclI AACGTT 1 cut(s) 480
AclWI GGATC 2 cut(s) 140, 153
AcoI YGGCCR 1 cut(s) 1048
AcuI CTGAAG 1 cut(s) 722
AfaI GTAC 5 cut(s) 46, 178, 666, 777, 983
AfiI CCNNNNNNNGG 3 cut(s) 395, 824, 1057
AflII CTTAAG 1 cut(s) 116
AgsI TTSAA 1 cut(s) 79
AjiI CACGTC 1 cut(s) 68
AjnI CCWGG 3 cut(s) 190, 388, 1056
AjuI GAANNNNNNNTTGG 2 cut(s) 779, 811
Alw21I GWGCWC 2 cut(s) 424, 1075
Alw26I GTCTC 4 cut(s) 58, 540, 614, 1185
AlwI GGATC 2 cut(s) 140, 153
AlwNI CAGNNNCTG 1 cut(s) 305
AoxI GGCC 1 cut(s) 1048
Asp700I GAANNNNTTC 2 cut(s) 469, 588
Asp718I GGTACC 1 cut(s) 176
AspLEI GCGC 2 cut(s) 190, 548
AspS9I GGNCC 2 cut(s) 613, 881
AsuHPI GGTGA 1 cut(s) 173
AvaII GGWCC 2 cut(s) 613, 881
BalI TGGCCA 1 cut(s) 1050
BamHI GGATCC 1 cut(s) 145
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 2 cut(s) 924, 1075
BauI CACGAG 2 cut(s) 1097, 1218
BbsI GAAGAC 1 cut(s) 710
Bbv12I GWGCWC 2 cut(s) 424, 1075
BbvI GCAGC 9 cut(s) 236, 301, 511, 535, 541, 563, 889, 1156, 1184
BccI CCATC 3 cut(s) 755, 871, 1059
BcgI CGANNNNNNTGC 2 cut(s) 1075, 1109
BciT130I CCWGG 3 cut(s) 192, 390, 1058
BclI TGATCA 1 cut(s) 379
BcoDI GTCTC 4 cut(s) 58, 540, 614, 1185
BfaI CTAG 1 cut(s) 747
BfmI CTRYAG 4 cut(s) 123, 549, 1065, 1170
BfoI RGCGCY 1 cut(s) 191
BfrI CTTAAG 1 cut(s) 116
BmcAI AGTACT 1 cut(s) 777
Bme1390I CCNGG 3 cut(s) 192, 390, 1058
Bme18I GGWCC 2 cut(s) 613, 881
BmgBI CACGTC 1 cut(s) 68
BmgT120I GGNCC 2 cut(s) 613, 881
BmiI GGNNCC 6 cut(s) 147, 178, 615, 727, 861, 1036
BmrFI CCNGG 3 cut(s) 192, 390, 1058
BmsI GCATC 1 cut(s) 977
BpiI GAAGAC 1 cut(s) 710
BpmI CTGGAG 3 cut(s) 378, 411, 789
BpuEI CTTGAG 1 cut(s) 560
BsaBI GATNNNNATC 1 cut(s) 939
BsaJI CCNNGG 2 cut(s) 1056, 1057
BsaXI ACNNNNNCTCC 2 cut(s) 1182, 1212
Bsc4I CCNNNNNNNGG 3 cut(s) 395, 824, 1057
Bse1I ACTGG 3 cut(s) 257, 856, 1162
Bse3DI GCAATG 3 cut(s) 213, 748, 1038
Bse8I GATNNNNATC 1 cut(s) 939
BseBI CCWGG 3 cut(s) 192, 390, 1058
BseDI CCNNGG 2 cut(s) 1056, 1057
BseGI GGATG 2 cut(s) 586, 1051
BseJI GATNNNNATC 1 cut(s) 939
BseLI CCNNNNNNNGG 3 cut(s) 395, 824, 1057
BseMI GCAATG 3 cut(s) 213, 748, 1038
BseMII CTCAG 3 cut(s) 212, 609, 986
BseNI ACTGG 3 cut(s) 257, 856, 1162
BseRI GAGGAG 1 cut(s) 1047
BseXI GCAGC 9 cut(s) 236, 301, 511, 535, 541, 563, 889, 1156, 1184
Bsh1236I CGCG 2 cut(s) 546, 563
BshFI GGCC 1 cut(s) 1050
BshNI GGYRCC 1 cut(s) 176
BsiHKAI GWGCWC 2 cut(s) 424, 1075
BslFI GGGAC 1 cut(s) 825
BslI CCNNNNNNNGG 3 cut(s) 395, 824, 1057
BsmAI GTCTC 4 cut(s) 58, 540, 614, 1185
BsmBI CGTCTC 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 825
BsnI GGCC 1 cut(s) 1050
Bsp1286I GDGCHC 3 cut(s) 424, 924, 1075
Bsp143I GATC 6 cut(s) 97, 145, 379, 753, 940, 951
BspACI CCGC 7 cut(s) 35, 48, 396, 527, 900, 1108, 1126
BspANI GGCC 1 cut(s) 1050
BspCNI CTCAG 3 cut(s) 211, 610, 985
BspFNI CGCG 2 cut(s) 546, 563
BspLI GGNNCC 6 cut(s) 147, 178, 615, 727, 861, 1036
BspMAI CTGCAG 2 cut(s) 553, 1174
BspPI GGATC 2 cut(s) 140, 153
BspQI GCTCTTC 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 176
BspTI CTTAAG 1 cut(s) 116
BsrDI GCAATG 3 cut(s) 213, 748, 1038
BsrI ACTGG 3 cut(s) 257, 856, 1162
BssECI CCNNGG 2 cut(s) 1056, 1057
BssMI GATC 6 cut(s) 97, 145, 379, 753, 940, 951
BssSI CACGAG 2 cut(s) 1097, 1218
Bst2BI CACGAG 2 cut(s) 1097, 1218
Bst2UI CCWGG 3 cut(s) 192, 390, 1058
Bst4CI ACNGT 5 cut(s) 181, 268, 662, 709, 725
Bst6I CTCTTC 3 cut(s) 93, 504, 782
BstAFI CTTAAG 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 739, 915
BstDEI CTNAG 5 cut(s) 198, 603, 618, 909, 972
BstF5I GGATG 2 cut(s) 586, 1051
BstFNI CGCG 2 cut(s) 546, 563
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 2 cut(s) 190, 548
BstKTI GATC 6 cut(s) 100, 148, 382, 756, 943, 954
BstMAI GTCTC 4 cut(s) 58, 540, 614, 1185
BstMBI GATC 6 cut(s) 97, 145, 379, 753, 940, 951
BstNI CCWGG 3 cut(s) 192, 390, 1058
BstSCI CCNGG 3 cut(s) 190, 388, 1056
BstSFI CTRYAG 4 cut(s) 123, 549, 1065, 1170
BstUI CGCG 2 cut(s) 546, 563
BstV1I GCAGC 9 cut(s) 236, 301, 511, 535, 541, 563, 889, 1156, 1184
BstV2I GAAGAC 1 cut(s) 710
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
BsuRI GGCC 1 cut(s) 1050
BtrI CACGTC 1 cut(s) 68
BtsCI GGATG 2 cut(s) 586, 1051
BtsIMutI CAGTG 2 cut(s) 763, 1155
Cac8I GCNNGC 2 cut(s) 739, 915
CaiI CAGNNNCTG 1 cut(s) 305
CfoI GCGC 2 cut(s) 190, 548
Cfr13I GGNCC 2 cut(s) 613, 881
CseI GACGC 1 cut(s) 552
Csp6I GTAC 5 cut(s) 45, 177, 665, 776, 982
CviAII CATG 2 cut(s) 404, 682
CviQI GTAC 5 cut(s) 45, 177, 665, 776, 982
DdeI CTNAG 5 cut(s) 198, 603, 618, 909, 972
DpnI GATC 6 cut(s) 99, 147, 381, 755, 942, 953
DpnII GATC 6 cut(s) 97, 145, 379, 753, 940, 951
DraI TTTAAA 1 cut(s) 715
EaeI YGGCCR 1 cut(s) 1048
Eam1104I CTCTTC 3 cut(s) 93, 504, 782
EarI CTCTTC 3 cut(s) 93, 504, 782
Ecl136II GAGCTC 1 cut(s) 1073
Eco24I GRGCYC 2 cut(s) 924, 1075
Eco32I GATATC 1 cut(s) 166
Eco47I GGWCC 2 cut(s) 613, 881
Eco53kI GAGCTC 1 cut(s) 1073
Eco57I CTGAAG 1 cut(s) 722
EcoICRI GAGCTC 1 cut(s) 1073
EcoRII CCWGG 3 cut(s) 190, 388, 1056
EcoRV GATATC 1 cut(s) 166
EcoT38I GRGCYC 2 cut(s) 924, 1075
Esp3I CGTCTC 1 cut(s) 58
FaeI CATG 2 cut(s) 407, 685
FaiI YATR 9 cut(s) 40, 384, 405, 521, 683, 803, 978, 1010, 1151
FalI AAGNNNNNCTT 6 cut(s) 72, 104, 124, 156, 695, 727
FaqI GGGAC 1 cut(s) 825
FatI CATG 2 cut(s) 403, 681
FauI CCCGC 1 cut(s) 907
FbaI TGATCA 1 cut(s) 379
FokI GGATG 2 cut(s) 593, 1038
FriOI GRGCYC 2 cut(s) 924, 1075
FspBI CTAG 1 cut(s) 747
GlaI GCGC 2 cut(s) 189, 547
GsuI CTGGAG 3 cut(s) 378, 411, 789
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 1 cut(s) 1050
HgaI GACGC 1 cut(s) 552
HhaI GCGC 2 cut(s) 190, 548
Hin1II CATG 2 cut(s) 407, 685
Hin6I GCGC 2 cut(s) 188, 546
HinP1I GCGC 2 cut(s) 188, 546
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HinfI GANTC 4 cut(s) 248, 365, 533, 1193
HphI GGTGA 1 cut(s) 173
Hpy166II GTNNAC 5 cut(s) 375, 572, 613, 665, 982
Hpy188I TCNGA 3 cut(s) 201, 475, 753
Hpy188III TCNNGA 5 cut(s) 92, 245, 288, 835, 949
Hpy8I GTNNAC 5 cut(s) 375, 572, 613, 665, 982
Hpy99I CGWCG 1 cut(s) 647
HpyAV CCTTC 2 cut(s) 628, 802
HpyCH4III ACNGT 5 cut(s) 181, 268, 662, 709, 725
HpyCH4IV ACGT 4 cut(s) 67, 480, 667, 927
HpyF3I CTNAG 5 cut(s) 198, 603, 618, 909, 972
HpySE526I ACGT 4 cut(s) 67, 480, 667, 927
Hsp92II CATG 2 cut(s) 407, 685
HspAI GCGC 2 cut(s) 188, 546
KpnI GGTACC 1 cut(s) 180
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 6 cut(s) 97, 145, 379, 753, 940, 951
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 4 cut(s) 770, 1034, 1070, 1166
Lsp1109I GCAGC 9 cut(s) 236, 301, 511, 535, 541, 563, 889, 1156, 1184
LweI GCATC 1 cut(s) 977
MaeI CTAG 1 cut(s) 747
MaeII ACGT 4 cut(s) 67, 480, 667, 927
MaeIII GTNAC 2 cut(s) 499, 847
MalI GATC 6 cut(s) 99, 147, 381, 755, 942, 953
MboI GATC 6 cut(s) 97, 145, 379, 753, 940, 951
MboII GAAGA 6 cut(s) 80, 92, 521, 715, 799, 817
MflI RGATCY 1 cut(s) 145
MhlI GDGCHC 3 cut(s) 424, 924, 1075
MlsI TGGCCA 1 cut(s) 1050
MluCI AATT 1 cut(s) 465
MluNI TGGCCA 1 cut(s) 1050
MlyI GAGTC 3 cut(s) 242, 542, 1187
MmeI TCCRAC 1 cut(s) 1236
MnlI CCTC 6 cut(s) 65, 386, 952, 1025, 1106, 1179
Mox20I TGGCCA 1 cut(s) 1050
MroXI GAANNNNTTC 2 cut(s) 469, 588
MscI TGGCCA 1 cut(s) 1050
MseI TTAA 3 cut(s) 117, 714, 1026
MslI CAYNNNNRTG 1 cut(s) 1160
Msp20I TGGCCA 1 cut(s) 1050
MspA1I CMGCKG 5 cut(s) 554, 737, 887, 902, 1175
MspCI CTTAAG 1 cut(s) 116
MspR9I CCNGG 3 cut(s) 192, 390, 1058
MvaI CCWGG 3 cut(s) 192, 390, 1058
MvnI CGCG 2 cut(s) 546, 563
NdeII GATC 6 cut(s) 97, 145, 379, 753, 940, 951
NlaIII CATG 2 cut(s) 407, 685
NlaIV GGNNCC 6 cut(s) 147, 178, 615, 727, 861, 1036
NmuCI GTSAC 1 cut(s) 499
PasI CCCWGGG 1 cut(s) 1057
PciSI GCTCTTC 1 cut(s) 504
PdmI GAANNNNTTC 2 cut(s) 469, 588
PfeI GAWTC 1 cut(s) 365
PflMI CCANNNNNTGG 1 cut(s) 1057
PfoI TCCNGGA 1 cut(s) 388
PleI GAGTC 3 cut(s) 242, 541, 1187
PpsI GAGTC 3 cut(s) 242, 541, 1187
Psp124BI GAGCTC 1 cut(s) 1075
Psp1406I AACGTT 1 cut(s) 480
Psp6I CCWGG 3 cut(s) 190, 388, 1056
PspGI CCWGG 3 cut(s) 190, 388, 1056
PspN4I GGNNCC 6 cut(s) 147, 178, 615, 727, 861, 1036
PspPI GGNCC 2 cut(s) 613, 881
PstI CTGCAG 2 cut(s) 553, 1174
PstNI CAGNNNCTG 1 cut(s) 305
PsuI RGATCY 1 cut(s) 145
PvuII CAGCTG 4 cut(s) 554, 737, 887, 1175
RsaI GTAC 5 cut(s) 46, 178, 666, 777, 983
RsaNI GTAC 5 cut(s) 45, 177, 665, 776, 982
RseI CAYNNNNRTG 1 cut(s) 1160
SacI GAGCTC 1 cut(s) 1075
SapI GCTCTTC 1 cut(s) 504
SaqAI TTAA 3 cut(s) 117, 714, 1026
Sau3AI GATC 6 cut(s) 97, 145, 379, 753, 940, 951
Sau96I GGNCC 2 cut(s) 613, 881
ScaI AGTACT 1 cut(s) 777
SchI GAGTC 3 cut(s) 242, 542, 1187
ScrFI CCNGG 3 cut(s) 192, 390, 1058
SduI GDGCHC 3 cut(s) 424, 924, 1075
SfaNI GCATC 1 cut(s) 977
SfcI CTRYAG 4 cut(s) 123, 549, 1065, 1170
SinI GGWCC 2 cut(s) 613, 881
SmiMI CAYNNNNRTG 1 cut(s) 1160
SmlI CTYRAG 2 cut(s) 116, 575
SmoI CTYRAG 2 cut(s) 116, 575
Sse9I AATT 1 cut(s) 465
SsiI CCGC 7 cut(s) 35, 48, 396, 527, 900, 1108, 1126
SspMI CTAG 1 cut(s) 747
SstI GAGCTC 1 cut(s) 1075
StyD4I CCNGG 3 cut(s) 190, 388, 1056
TaaI ACNGT 5 cut(s) 181, 268, 662, 709, 725
TaiI ACGT 4 cut(s) 70, 483, 670, 930
TaqI TCGA 2 cut(s) 948, 1075
TasI AATT 1 cut(s) 465
TatI WGTACW 2 cut(s) 775, 981
TauI GCSGC 3 cut(s) 38, 399, 1128
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 3 cut(s) 117, 714, 1026
Tru9I TTAA 3 cut(s) 117, 714, 1026
TscAI CASTG 2 cut(s) 763, 1162
TseFI GTSAC 1 cut(s) 499
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 5 cut(s) 392, 597, 800, 818, 1118
TspGWI ACGGA 1 cut(s) 241
TspRI CASTG 2 cut(s) 763, 1162
Van91I CCANNNNNTGG 1 cut(s) 1057
Vha464I CTTAAG 1 cut(s) 116
VpaK11BI GGWCC 2 cut(s) 613, 881
XcmI CCANNNNNNNNNTGG 1 cut(s) 1219
XmnI GAANNNNTTC 2 cut(s) 469, 588
XspI CTAG 1 cut(s) 747
ZrmI AGTACT 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.