Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003035

Physical Location & Seq

Genomic Coordinates Forward (+)

20255173 .. 20259982

Gene Structure 4810 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G49840.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1827 bp

ATGTTTTGTAGCTTATCCATTTCCAGATTTGTCTCGAGGAAGACAATAACATCATCATCTCTTCTCTCTCGCAGTTTCAGATTCCTTCTCTCCGTTGATTCTCCTCCTCACATCCCTCTTCTACGCCCTTCTTCCAACACCCTAATCCCTTCCTCTTCCTTCTCCCGCCGTATTTGGGACTCCTGCAGCGGCGGCGGCGGAGGTGGCGGCGGAGATGATTATGATCACATCAGAAGTGACGTCAATTGTCCTCGTTGCTCTGCTCAGATGCACGTCATCTTCTCAAATCGTCCATTGTCTCTAACAGCAAGAGAACCAGGGATCTATCAAGCTGTTAATTTCTGTTCTCAATGCAAAACAGCTTTTTACTTTAGACCATTCAAGCTCTCTCCTTTACAAGGGAGTTTTATTGAACTCGGGAAAGTTAAAGGAACTGATGATGACCATGATGATGATGATGATGATCAGAAATCTTTCCCTAGGAATTGGAAAATTCAAGGTCTTAGAAGCGATGAAGATGGTGAGGATGCTGATGAGGAGGAGGATGAAAGTAATGGTGGTGATAAAGAGAAGCAATCTGTGATTAAGCTTCCAACTCCTAAGGAAATTTGTCAAGGGCTTGATGAGTTTGTCATTGGTCAAGAGAAGGCTAAGAAGGTTCTTTCTGTGGCTGTTTATAATCATTACAAAAGAATATATCATGCTTCTAGGAAAAAAGGGTCTGCTTCAGAATCATATAACATTGATATGGAGGATGATAATATTGATCACGTGGAGTTGGATAAGAGTAATGTGTTGTTGCTTGGTCCTACTGGTTCAGGAAAGACTTTACTGGCAAAAACTTTGGCGCGTATTGTCAATGTGCCATTCGCTATTGCTGATGCGACTTCACTAACTCAGGCAAGTTATGTTGGTGAAGATGTGGAATCAATATTGTACAAACTATATGTGGAAGCTGGGTGCAATGTAGAAGAAGCTCAAAGGGGAATTGTGTACATTGATGAAGTTGATAAAATGACTATGAAGTCTCATAGCTCAAATGGTGGTAGAGATGTCTCTGGAGAAGGTGTTCAACAGTCATTGCTAAAATTGCTGGAAGGAACTGTGGTCAGTGTCCCGATTCCCGAGAAAGGATTACGGAGAGATCCTCGAGGAGATAGCATTCAGATGGATACAAAAGACATCCTTTTTATTTGTGGTGGTGCATTTATCGATCTGGAGAAAACTGTTTCTGAAAGGCAGCATGATGCCTCTATTGGTTTTGGTGCTTCCGTTCGTACAAACATGAGTACTTCTGGGTTAAGTAGTGCTGCTGTAACATCCTCCTTGTTGGAATCTTTACAAAGTGAAGATCTTGTTGCGTATGGTCTCATTCCTGAGTTTGTTGGAAGGTTACCCATCTTAGTGAGCTTATCCGCATTAAACGAAGATCAACTTGTACAGGTTCTCACAGAGCCTAAAAGTGCATTGGGTAAACAGTACAAGAAATTGTTTCGCATGAACAATGTCCAACTGCAATTTACTGAAGGAGCAACGAGGCTAATCGCAAGGAAAGCTATGTCGAAGAACACTGGTGCACGTGGCTTACGGTCTATACTGGAAAGTATTCTCACCGAAGCAATGTTTGAGGTACCAGATAGCATCACGGAAGGATCACAGAGTATAAAGGCAGTTCTGGTGGATGAAGAGGCTGTTGGTTCAGTTGGGTCCCCAGGATGTGGCGCAAAAATTCTTAAAGGAGATAATGTGTTGCAACAATTTGTTGAAGAAGCAGAGTCAAAGGAGAAGAGTAAGGAGGATGAAGCTAAGAGAGCTCAGAGTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004176 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006508 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019538 GO:0030163 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042623 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0070013 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04112 map04112

Pfam Domains

Protein Families

AAA_2 ClpB_D2-small

Protein Analysis

608

Amino Acids

66.39

Weight (kDa)

5.21

Isoelectric Point (pI)

50.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM	PF00493	261 - 344	1.7e-06	MCM P-loop domain
AAA_2	PF07724	262 - 467	8.9e-27	AAA domain (Cdc48 subfamily)
AAA	PF00004	265 - 370	2.2e-13	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	474 - 543	6.1e-10	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003035)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49840 AT5G49840 AT5G49840 AT5G49840
fragaria_vesca	FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740
malus_domestica	MD08G1248400.v1.1
prunus_persica	Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1
pyrus_communis	pycom08g21520
rosa_chinensis	RchiOBHm_Chr7g0219511
rosa_laevigata	RLG00000002286
rosa_multiflora	Rmu_sc0003047.1_g000013
rosa_roxburghii	Rroxscaffold_3G00240000
rosa_rugosa	Rorug07G0185800 Rorug07G0185900
rosa_samantha	Rh7AG328400 Rh7BG318900 Rh7CG345800 Rh7DG324800
rosa_wichuraiana	Rw7G027750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	678
AasI	GACNNNNNNGTC	1 cut(s)	1024
AatII	GACGTC	1 cut(s)	243
AbsI	CCTCGAGG	1 cut(s)	1149
Acc65I	GGTACC	1 cut(s)	1630
AccB1I	GGYRCC	1 cut(s)	1630
AccB7I	CCANNNNNTGG	1 cut(s)	1718
AccII	CGCG	1 cut(s)	850
AciI	CCGC	8 cut(s)	166, 189, 192, 195, 198, 207, 210, 1416
AclWI	GGATC	3 cut(s)	329, 1139, 1660
AcsI	RAATTY	3 cut(s)	492, 606, 1728
AcuI	CTGAAG	2 cut(s)	711, 1545
AcvI	CACGTG	2 cut(s)	772, 1580
AcyI	GRCGYC	1 cut(s)	240
AfaI	GTAC	7 cut(s)	938, 995, 1279, 1291, 1440, 1481, 1632
AfiI	CCNNNNNNNGG	4 cut(s)	175, 398, 1130, 1718
AgsI	TTSAA	5 cut(s)	382, 413, 497, 1073, 1766
AjiI	CACGTC	1 cut(s)	274
AjnI	CCWGG	2 cut(s)	316, 1711
AjuI	GAANNNNNNNTTGG	2 cut(s)	1677, 1709
AloI	GAACNNNNNNTCC	2 cut(s)	1053, 1085
Alw21I	GWGCWC	2 cut(s)	1579, 1816
Alw26I	GTCTC	5 cut(s)	37, 303, 1032, 1060, 1373
Alw44I	GTGCAC	1 cut(s)	1575
AlwI	GGATC	3 cut(s)	329, 1139, 1660
Ama87I	CYCGRG	4 cut(s)	34, 416, 1124, 1149
ApaLI	GTGCAC	1 cut(s)	1575
ApeKI	GCWGC	3 cut(s)	186, 1240, 1310
ApoI	RAATTY	3 cut(s)	492, 606, 1728
Asp700I	GAANNNNTTC	3 cut(s)	473, 1068, 1605
Asp718I	GGTACC	1 cut(s)	1630
AspA2I	CCTAGG	1 cut(s)	479
AspLEI	GCGC	2 cut(s)	850, 1724
AspS9I	GGNCC	2 cut(s)	806, 1707
AsuHPI	GGTGA	4 cut(s)	533, 572, 926, 1603
AvaI	CYCGRG	4 cut(s)	34, 416, 1124, 1149
AvaII	GGWCC	2 cut(s)	806, 1707
AvrII	CCTAGG	1 cut(s)	479
AxyI	CCTNAGG	1 cut(s)	600
BaeGI	GKGCMC	1 cut(s)	1579
BanI	GGYRCC	1 cut(s)	1630
BanII	GRGCYC	1 cut(s)	1816
BbrPI	CACGTG	2 cut(s)	772, 1580
BbsI	GAAGAC	1 cut(s)	47
Bbv12I	GWGCWC	2 cut(s)	1579, 1816
BbvI	GCAGC	3 cut(s)	198, 1252, 1297
BccI	CCATC	3 cut(s)	512, 1162, 1406
BceAI	ACGGC	1 cut(s)	153
BciT130I	CCWGG	2 cut(s)	318, 1713
BciVI	GTATCC	1 cut(s)	1165
BclI	TGATCA	3 cut(s)	223, 463, 766
BcoDI	GTCTC	5 cut(s)	37, 303, 1032, 1060, 1373
BfaI	CTAG	2 cut(s)	480, 708
BfmI	CTRYAG	1 cut(s)	184
BfuI	GTATCC	1 cut(s)	1165
BglII	AGATCT	1 cut(s)	1351
BisI	GCNGC	7 cut(s)	187, 190, 193, 196, 208, 1241, 1311
BlnI	CCTAGG	1 cut(s)	479
BlsI	GCNGC	7 cut(s)	188, 191, 194, 197, 209, 1242, 1312
BmcAI	AGTACT	1 cut(s)	1291
Bme1390I	CCNGG	2 cut(s)	318, 1713
Bme18I	GGWCC	2 cut(s)	806, 1707
BmeT110I	CYCGRG	4 cut(s)	34, 416, 1124, 1149
BmgBI	CACGTC	1 cut(s)	274
BmgT120I	GGNCC	2 cut(s)	806, 1707
BmiI	GGNNCC	3 cut(s)	1632, 1708, 1709
BmrFI	CCNGG	2 cut(s)	318, 1713
BmsI	GCATC	5 cut(s)	258, 517, 871, 1237, 1650
BpiI	GAAGAC	1 cut(s)	47
BplI	GAGNNNNNCTC	2 cut(s)	1132, 1164
BpmI	CTGGAG	2 cut(s)	1080, 1238
Bsa29I	ATCGAT	1 cut(s)	1212
BsaAI	YACGTR	2 cut(s)	772, 1580
BsaBI	GATNNNNATC	2 cut(s)	222, 462
BsaHI	GRCGYC	1 cut(s)	240
BsaI	GGTCTC	1 cut(s)	1373
BsaJI	CCNNGG	3 cut(s)	317, 479, 1711
BsaXI	ACNNNNNCTCC	4 cut(s)	1053, 1083, 1731, 1761
Bsc4I	CCNNNNNNNGG	4 cut(s)	175, 398, 1130, 1718
Bse1I	ACTGG	4 cut(s)	817, 837, 1576, 1602
Bse21I	CCTNAGG	1 cut(s)	600
Bse3DI	GCAATG	3 cut(s)	970, 1079, 1626
Bse8I	GATNNNNATC	2 cut(s)	222, 462
BseBI	CCWGG	2 cut(s)	318, 1713
BseCI	ATCGAT	1 cut(s)	1212
BseDI	CCNNGG	3 cut(s)	317, 479, 1711
BseGI	GGATG	9 cut(s)	111, 532, 550, 760, 1182, 1319, 1687, 1721, 1804
BseJI	GATNNNNATC	2 cut(s)	222, 462
BseLI	CCNNNNNNNGG	4 cut(s)	175, 398, 1130, 1718
BseMI	GCAATG	3 cut(s)	970, 1079, 1626
BseMII	CTCAG	3 cut(s)	278, 911, 1368
BseNI	ACTGG	4 cut(s)	817, 837, 1576, 1602
BseRI	GAGGAG	5 cut(s)	93, 96, 551, 554, 1167
BseSI	GKGCMC	1 cut(s)	1579
BseXI	GCAGC	3 cut(s)	198, 1252, 1297
BseYI	CCCAGC	1 cut(s)	956
Bsh1236I	CGCG	1 cut(s)	850
BshNI	GGYRCC	1 cut(s)	1630
BshVI	ATCGAT	1 cut(s)	1212
BsiHKAI	GWGCWC	2 cut(s)	1579, 1816
BsiHKCI	CYCGRG	4 cut(s)	34, 416, 1124, 1149
BslFI	GGGAC	3 cut(s)	191, 1100, 1693
BslI	CCNNNNNNNGG	4 cut(s)	175, 398, 1130, 1718
BsmAI	GTCTC	5 cut(s)	37, 303, 1032, 1060, 1373
BsmFI	GGGAC	3 cut(s)	191, 1100, 1693
BsmI	GAATGC	1 cut(s)	1161
Bso31I	GGTCTC	1 cut(s)	1373
BsoBI	CYCGRG	4 cut(s)	34, 416, 1124, 1149
Bsp1286I	GDGCHC	2 cut(s)	1579, 1816
Bsp1407I	TGTACA	3 cut(s)	936, 993, 1438
Bsp143I	GATC	9 cut(s)	223, 321, 463, 766, 1144, 1213, 1351, 1429, 1652
BspACI	CCGC	8 cut(s)	166, 189, 192, 195, 198, 207, 210, 1416
BspCNI	CTCAG	3 cut(s)	277, 910, 1369
BspDI	ATCGAT	1 cut(s)	1212
BspFNI	CGCG	1 cut(s)	850
BspLI	GGNNCC	3 cut(s)	1632, 1708, 1709
BspMAI	CTGCAG	1 cut(s)	188
BspPI	GGATC	3 cut(s)	329, 1139, 1660
BspT107I	GGYRCC	1 cut(s)	1630
BspTNI	GGTCTC	1 cut(s)	1373
BsrDI	GCAATG	3 cut(s)	970, 1079, 1626
BsrGI	TGTACA	3 cut(s)	936, 993, 1438
BsrI	ACTGG	4 cut(s)	817, 837, 1576, 1602
BssECI	CCNNGG	3 cut(s)	317, 479, 1711
BssMI	GATC	9 cut(s)	223, 321, 463, 766, 1144, 1213, 1351, 1429, 1652
BssNI	GRCGYC	1 cut(s)	240
BssT1I	CCWWGG	1 cut(s)	479
Bst2UI	CCWGG	2 cut(s)	318, 1713
Bst4CI	ACNGT	5 cut(s)	1077, 1105, 1228, 1479, 1590
Bst6I	CTCTTC	5 cut(s)	66, 123, 160, 1680, 1781
BstACI	GRCGYC	1 cut(s)	240
BstAUI	TGTACA	3 cut(s)	936, 993, 1438
BstBAI	YACGTR	2 cut(s)	772, 1580
BstDEI	CTNAG	9 cut(s)	264, 503, 600, 651, 897, 1377, 1402, 1806, 1815
BstEII	GGTNACC	1 cut(s)	1392
BstENI	CCTNNNNNAGG	1 cut(s)	396
BstF5I	GGATG	9 cut(s)	111, 532, 550, 760, 1182, 1319, 1687, 1721, 1804
BstFNI	CGCG	1 cut(s)	850
BstHHI	GCGC	2 cut(s)	850, 1724
BstKTI	GATC	9 cut(s)	226, 324, 466, 769, 1147, 1216, 1354, 1432, 1655
BstMAI	GTCTC	5 cut(s)	37, 303, 1032, 1060, 1373
BstMBI	GATC	9 cut(s)	223, 321, 463, 766, 1144, 1213, 1351, 1429, 1652
BstMWI	GCNNNNNNNGC	6 cut(s)	192, 195, 204, 1090, 1553, 1811
BstNI	CCWGG	2 cut(s)	318, 1713
BstPI	GGTNACC	1 cut(s)	1392
BstSCI	CCNGG	2 cut(s)	316, 1711
BstSFI	CTRYAG	1 cut(s)	184
BstSLI	GKGCMC	1 cut(s)	1579
BstUI	CGCG	1 cut(s)	850
BstV1I	GCAGC	3 cut(s)	198, 1252, 1297
BstV2I	GAAGAC	1 cut(s)	47
BstX2I	RGATCY	3 cut(s)	321, 1144, 1351
BstYI	RGATCY	3 cut(s)	321, 1144, 1351
Bsu15I	ATCGAT	1 cut(s)	1212
Bsu36I	CCTNAGG	1 cut(s)	600
BsuI	GTATCC	1 cut(s)	1165
BsuTUI	ATCGAT	1 cut(s)	1212
BtgZI	GCGATG	1 cut(s)	525
BtrI	CACGTC	1 cut(s)	274
BtsCI	GGATG	9 cut(s)	111, 532, 550, 760, 1182, 1319, 1687, 1721, 1804
BtsIMutI	CAGTG	2 cut(s)	1117, 1569
CfoI	GCGC	2 cut(s)	850, 1724
Cfr13I	GGNCC	2 cut(s)	806, 1707
ClaI	ATCGAT	1 cut(s)	1212
Csp6I	GTAC	7 cut(s)	937, 994, 1278, 1290, 1439, 1480, 1631
CviAII	CATG	5 cut(s)	446, 701, 1244, 1285, 1498
CviQI	GTAC	7 cut(s)	937, 994, 1278, 1290, 1439, 1480, 1631
DdeI	CTNAG	9 cut(s)	264, 503, 600, 651, 897, 1377, 1402, 1806, 1815
DpnI	GATC	9 cut(s)	225, 323, 465, 768, 1146, 1215, 1353, 1431, 1654
DpnII	GATC	9 cut(s)	223, 321, 463, 766, 1144, 1213, 1351, 1429, 1652
DrdI	GACNNNNNNGTC	1 cut(s)	1024
DseDI	GACNNNNNNGTC	1 cut(s)	1024
Eam1104I	CTCTTC	5 cut(s)	66, 123, 160, 1680, 1781
EarI	CTCTTC	5 cut(s)	66, 123, 160, 1680, 1781
EciI	GGCGGA	2 cut(s)	213, 225
Ecl136II	GAGCTC	1 cut(s)	1814
Eco130I	CCWWGG	1 cut(s)	479
Eco24I	GRGCYC	1 cut(s)	1816
Eco31I	GGTCTC	1 cut(s)	1373
Eco47I	GGWCC	2 cut(s)	806, 1707
Eco53kI	GAGCTC	1 cut(s)	1814
Eco57I	CTGAAG	2 cut(s)	711, 1545
Eco72I	CACGTG	2 cut(s)	772, 1580
Eco81I	CCTNAGG	1 cut(s)	600
Eco88I	CYCGRG	4 cut(s)	34, 416, 1124, 1149
Eco91I	GGTNACC	1 cut(s)	1392
EcoICRI	GAGCTC	1 cut(s)	1814
EcoNI	CCTNNNNNAGG	1 cut(s)	396
EcoO109I	RGGNCCY	1 cut(s)	1707
EcoO65I	GGTNACC	1 cut(s)	1392
EcoRII	CCWGG	2 cut(s)	316, 1711
EcoT14I	CCWWGG	1 cut(s)	479
EcoT38I	GRGCYC	1 cut(s)	1816
ErhI	CCWWGG	1 cut(s)	479
FaeI	CATG	5 cut(s)	449, 704, 1247, 1288, 1501
FalI	AAGNNNNNCTT	4 cut(s)	1170, 1202, 1419, 1451
FaqI	GGGAC	3 cut(s)	191, 1100, 1693
FatI	CATG	5 cut(s)	445, 700, 1243, 1284, 1497
FauI	CCCGC	1 cut(s)	173
FbaI	TGATCA	3 cut(s)	223, 463, 766
Fnu4HI	GCNGC	7 cut(s)	187, 190, 193, 196, 208, 1241, 1311
FokI	GGATG	9 cut(s)	98, 539, 557, 767, 1169, 1306, 1694, 1728, 1811
FriOI	GRGCYC	1 cut(s)	1816
Fsp4HI	GCNGC	7 cut(s)	187, 190, 193, 196, 208, 1241, 1311
FspBI	CTAG	2 cut(s)	480, 708
GlaI	GCGC	2 cut(s)	849, 1723
GluI	GCNGC	7 cut(s)	187, 190, 193, 196, 208, 1241, 1311
GsaI	CCCAGC	1 cut(s)	960
GsuI	CTGGAG	2 cut(s)	1080, 1238
HhaI	GCGC	2 cut(s)	850, 1724
Hin1I	GRCGYC	1 cut(s)	240
Hin1II	CATG	5 cut(s)	449, 704, 1247, 1288, 1501
Hin6I	GCGC	2 cut(s)	848, 1722
HinP1I	GCGC	2 cut(s)	848, 1722
HindIII	AAGCTT	1 cut(s)	587
HinfI	GANTC	8 cut(s)	81, 98, 179, 731, 926, 1120, 1334, 1775
HphI	GGTGA	4 cut(s)	533, 572, 926, 1603
Hpy166II	GTNNAC	3 cut(s)	994, 1475, 1577
Hpy188I	TCNGA	8 cut(s)	80, 233, 267, 468, 730, 1167, 1234, 1818
Hpy8I	GTNNAC	3 cut(s)	994, 1475, 1577
HpyCH4III	ACNGT	5 cut(s)	1077, 1105, 1228, 1479, 1590
HpyCH4IV	ACGT	4 cut(s)	240, 273, 771, 1579
HpyCH4V	TGCA	9 cut(s)	186, 271, 354, 963, 1205, 1466, 1516, 1577, 1753
HpyF10VI	GCNNNNNNNGC	6 cut(s)	192, 195, 204, 1090, 1553, 1811
HpyF3I	CTNAG	9 cut(s)	264, 503, 600, 651, 897, 1377, 1402, 1806, 1815
HpySE526I	ACGT	4 cut(s)	240, 273, 771, 1579
Hsp92I	GRCGYC	1 cut(s)	240
Hsp92II	CATG	5 cut(s)	449, 704, 1247, 1288, 1501
HspAI	GCGC	2 cut(s)	848, 1722
KflI	GGGWCCC	1 cut(s)	1707
KpnI	GGTACC	1 cut(s)	1634
Ksp22I	TGATCA	3 cut(s)	223, 463, 766
Kzo9I	GATC	9 cut(s)	223, 321, 463, 766, 1144, 1213, 1351, 1429, 1652
LmnI	GCTCC	1 cut(s)	1529
Lsp1109I	GCAGC	3 cut(s)	198, 1252, 1297
LweI	GCATC	5 cut(s)	258, 517, 871, 1237, 1650
MaeI	CTAG	2 cut(s)	480, 708
MaeII	ACGT	4 cut(s)	240, 273, 771, 1579
MaeIII	GTNAC	3 cut(s)	236, 1315, 1392
MalI	GATC	9 cut(s)	225, 323, 465, 768, 1146, 1215, 1353, 1431, 1654
MboI	GATC	9 cut(s)	223, 321, 463, 766, 1144, 1213, 1351, 1429, 1652
MfeI	CAATTG	1 cut(s)	244
MflI	RGATCY	3 cut(s)	321, 1144, 1351
MhlI	GDGCHC	2 cut(s)	1579, 1816
MlyI	GAGTC	2 cut(s)	173, 1784
MmeI	TCCRAC	6 cut(s)	159, 617, 759, 1311, 1366, 1534
MroXI	GAANNNNTTC	3 cut(s)	473, 1068, 1605
MseI	TTAA	6 cut(s)	336, 426, 585, 1301, 1421, 1734
MslI	CAYNNNNRTG	4 cut(s)	450, 746, 1166, 1403
MspA1I	CMGCKG	1 cut(s)	189
MspR9I	CCNGG	2 cut(s)	318, 1713
MunI	CAATTG	1 cut(s)	244
Mva1269I	GAATGC	1 cut(s)	1161
MvaI	CCWGG	2 cut(s)	318, 1713
MvnI	CGCG	1 cut(s)	850
MwoI	GCNNNNNNNGC	6 cut(s)	192, 195, 204, 1090, 1553, 1811
NdeII	GATC	9 cut(s)	223, 321, 463, 766, 1144, 1213, 1351, 1429, 1652
NlaIII	CATG	5 cut(s)	449, 704, 1247, 1288, 1501
NlaIV	GGNNCC	3 cut(s)	1632, 1708, 1709
NmuCI	GTSAC	1 cut(s)	236
PaeR7I	CTCGAG	2 cut(s)	34, 1149
PctI	GAATGC	1 cut(s)	1161
PdmI	GAANNNNTTC	3 cut(s)	473, 1068, 1605
PfeI	GAWTC	6 cut(s)	81, 98, 731, 926, 1120, 1334
PflMI	CCANNNNNTGG	1 cut(s)	1718
PkrI	GCNGC	7 cut(s)	188, 191, 194, 197, 209, 1242, 1312
PleI	GAGTC	2 cut(s)	173, 1783
PmaCI	CACGTG	2 cut(s)	772, 1580
PmlI	CACGTG	2 cut(s)	772, 1580
PpsI	GAGTC	2 cut(s)	173, 1783
Ppu21I	YACGTR	2 cut(s)	772, 1580
PpuMI	RGGWCCY	1 cut(s)	1707
PsiI	TTATAA	1 cut(s)	678
Psp124BI	GAGCTC	1 cut(s)	1816
Psp5II	RGGWCCY	1 cut(s)	1707
Psp6I	CCWGG	2 cut(s)	316, 1711
PspCI	CACGTG	2 cut(s)	772, 1580
PspEI	GGTNACC	1 cut(s)	1392
PspFI	CCCAGC	1 cut(s)	956
PspGI	CCWGG	2 cut(s)	316, 1711
PspN4I	GGNNCC	3 cut(s)	1632, 1708, 1709
PspPI	GGNCC	2 cut(s)	806, 1707
PspPPI	RGGWCCY	1 cut(s)	1707
PspXI	VCTCGAGB	1 cut(s)	1149
PstI	CTGCAG	1 cut(s)	188
PsuI	RGATCY	3 cut(s)	321, 1144, 1351
RsaI	GTAC	7 cut(s)	938, 995, 1279, 1291, 1440, 1481, 1632
RsaNI	GTAC	7 cut(s)	937, 994, 1278, 1290, 1439, 1480, 1631
RseI	CAYNNNNRTG	4 cut(s)	450, 746, 1166, 1403
SacI	GAGCTC	1 cut(s)	1816
SaqAI	TTAA	6 cut(s)	336, 426, 585, 1301, 1421, 1734
SatI	GCNGC	7 cut(s)	187, 190, 193, 196, 208, 1241, 1311
Sau3AI	GATC	9 cut(s)	223, 321, 463, 766, 1144, 1213, 1351, 1429, 1652
Sau96I	GGNCC	2 cut(s)	806, 1707
ScaI	AGTACT	1 cut(s)	1291
SchI	GAGTC	2 cut(s)	173, 1784
ScrFI	CCNGG	2 cut(s)	318, 1713
SduI	GDGCHC	2 cut(s)	1579, 1816
SfaNI	GCATC	5 cut(s)	258, 517, 871, 1237, 1650
SfcI	CTRYAG	1 cut(s)	184
Sfr274I	CTCGAG	2 cut(s)	34, 1149
SinI	GGWCC	2 cut(s)	806, 1707
SlaI	CTCGAG	2 cut(s)	34, 1149
SmiMI	CAYNNNNRTG	4 cut(s)	450, 746, 1166, 1403
SmlI	CTYRAG	2 cut(s)	34, 1149
SmoI	CTYRAG	2 cut(s)	34, 1149
SsiI	CCGC	8 cut(s)	166, 189, 192, 195, 198, 207, 210, 1416
SspI	AATATT	2 cut(s)	763, 933
SspMI	CTAG	2 cut(s)	480, 708
SstI	GAGCTC	1 cut(s)	1816
StyD4I	CCNGG	2 cut(s)	316, 1711
StyI	CCWWGG	1 cut(s)	479
TaaI	ACNGT	5 cut(s)	1077, 1105, 1228, 1479, 1590
TaiI	ACGT	4 cut(s)	243, 276, 774, 1582
TaqI	TCGA	4 cut(s)	35, 1150, 1212, 1562
TatI	WGTACW	5 cut(s)	936, 993, 1289, 1438, 1479
TauI	GCSGC	4 cut(s)	192, 195, 198, 210
TfiI	GAWTC	6 cut(s)	81, 98, 731, 926, 1120, 1334
Tru1I	TTAA	6 cut(s)	336, 426, 585, 1301, 1421, 1734
Tru9I	TTAA	6 cut(s)	336, 426, 585, 1301, 1421, 1734
TscAI	CASTG	2 cut(s)	1117, 1576
TseFI	GTSAC	1 cut(s)	236
TseI	GCWGC	3 cut(s)	186, 1240, 1310
Tsp45I	GTSAC	1 cut(s)	236
TspDTI	ATGAA	7 cut(s)	528, 561, 1017, 1037, 1514, 1698, 1815
TspGWI	ACGGA	4 cut(s)	82, 1153, 1261, 1661
TspRI	CASTG	2 cut(s)	1117, 1576
Van91I	CCANNNNNTGG	1 cut(s)	1718
VneI	GTGCAC	1 cut(s)	1575
VpaK11BI	GGWCC	2 cut(s)	806, 1707
XagI	CCTNNNNNAGG	1 cut(s)	396
XapI	RAATTY	3 cut(s)	492, 606, 1728
XhoI	CTCGAG	2 cut(s)	34, 1149
XmaJI	CCTAGG	1 cut(s)	479
XmnI	GAANNNNTTC	3 cut(s)	473, 1068, 1605
XspI	CTAG	2 cut(s)	480, 708
ZraI	GACGTC	1 cut(s)	241
ZrmI	AGTACT	1 cut(s)	1291

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.