Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003035

Physical Location & Seq

Genomic Coordinates Reverse (-)

31242560 .. 31248592

Gene Structure 6033 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1248400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1800 bp

ATGTTTCAGTCTCTTCTTCGATATAGATTCAGCAGCACGACCCCTTCATCTCTCAAACGAATAATCACCGCAGTCCCCAATGTCTACAAAACCCACGCACTCTCACTCCTCCCCCCTAAACAACATCTGCAGCAGCGGCGGCGGTGCCACGACTGGGAGGGCACGCTTGGAGGCGGAGGCGGAGGAGGAGGAGGAGGAGGAGGAGGGAGTGGAGAGTACGATCACATTAGGGCCGAAGTCAACTGTCCCCGGTGCTCCAAACAAATGGCGGTGCTCTTCTCCACCCGACCCCTCTCCATCACCAGCCGAGAGACGGGGCTCTACCAGGCCGTCAATCTCTGCCCCAATTGCAGGACCGCCTTCTACTTCAGGCCCTTGAAGCTCGTCCCTCTACACGGCACCTTCGTGGAGATTGGTCGCCTTAAAGACTTAAAGCCCCCCACCAATTCCTTCGACGCAAAAACAGAGCCATGCGCTGTTGATGATAAAACGCCCTCGTCTTCTCCGCCCAGGGACTTGCCCACCCCCAAGTTCATCTGTAAGGCGCTTGATGAATTTGTTATCGGCCAACAAAGAGCCAAAAAGGTGCTTTCCGTTGCAGTTTACAACCACTATAAAAGGATACTCCATGCTTCCCCAAAGACGATGAGGTCTGGTGCAGAATCAGTTGATTATAAACTTGAAGGTGTACATGACAGTGACTCTGTTGAACTGGAGAAGAGCAACGTATTGCTGATGGGTCCCACTGGTTCAGGGAAGACGTTACTTGCAAAAACACTAGCTCGTGTTGTCAATGTGCCGTTTGCCATTGCTGATGCAACAACATTAACACAGGCAGGTTATGTTGGAGAAGATGTGGAATCAATATTGTATAGACTGCTTATGGAAGCTGACTTTGATGTGGAAGCAGCTCAGAAAGGGATTGTCTACATTGATGAAGTTGATAAGATAACTAAGAAGGTTGAGAGCTTGAGCAGAGATGTATCCGGGGAGGGTGTCCAACAGGCATTGCTGAAAATGTTAGAAGGAACCGTAGTTAGCGTTTCTGATAAAGGAGCTCGGAAGCATTCTCGTGGTGATAGCATCCAGATGGATACCAACAATATCCTTTTCATATGTGGTGGGGCATTTGTTGGTTTGGAGAAGACCATTTCAGAAAGACTCCAAGATTCTTCCATTGGATTTGGTGTACCAGTTCGTGCAAACATGAGGACCAGTGGCTTGACTGATGCCATAGTGACATCATCTTTGTTGGAATATGTAGAAAGTGGAGATCTTATTGCGTACGGCCTGATACCTGAATTTGTTGGCCGCTTTCCTGTTTTAGCTAGTTTGTCATCTTTAGATGAGGACCAACTTGTTCAGGTGCTCACAGAACCTAAAAATGCTCTTGGTAAACAATACAAGAAGATGTTCAACATGAACAATGTCCAATTACATTTTACTGATAATGCATTGAGATTGATTGCAAAGAAGGCAATGGCAAAGAACGCTGGGGCTCGAGGTTTGAGAGCAATATTGGAAAATATTCTAACAGAAGCAATGTTTGAGGTTCCAGACATGAAGACGGAAACCAGTGCATATACAGTTTTGGTGGATGAAGAAGCTGTTGGGTCAGAGGACACTTGTGGACTTGGAGCTAAAATTCTTAGTGGGGATGGTGCATTGGAGAAATTTCTTCGGGAAACAAAGTCAGGAGAGTGCATGGGAAAGCGCGGGTTGGATGGAGATGAGTGTTTACGAGAAGTCTTAGATTTTCAATCAAGAGCCACGGCGGGGGGCTTGTCTGTGGTGGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04112 map04112

Pfam Domains

Protein Families

AAA_2 ClpB_D2-small

Protein Analysis

600

Amino Acids

65.01

Weight (kDa)

7.12

Isoelectric Point (pI)

41.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_2	PF07724	239 - 441	1.2e-29	AAA domain (Cdc48 subfamily)
AAA	PF00004	243 - 347	5.4e-14	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	448 - 517	6.3e-10	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003035)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49840 AT5G49840 AT5G49840 AT5G49840
fragaria_vesca	FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740 FvH4_5g27740
malus_domestica	MD08G1248400.v1.1
prunus_persica	Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1 Prupe.1G585800_v2.0.a1
pyrus_communis	pycom08g21520
rosa_chinensis	RchiOBHm_Chr7g0219511
rosa_laevigata	RLG00000002286
rosa_multiflora	Rmu_sc0003047.1_g000013
rosa_roxburghii	Rroxscaffold_3G00240000
rosa_rugosa	Rorug07G0185800 Rorug07G0185900
rosa_samantha	Rh7AG328400 Rh7BG318900 Rh7CG345800 Rh7DG324800
rosa_wichuraiana	Rw7G027750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	675
AasI	GACNNNNNNGTC	1 cut(s)	649
Acc36I	ACCTGC	1 cut(s)	827
AccB1I	GGYRCC	2 cut(s)	144, 398
AccI	GTMKAC	2 cut(s)	84, 927
AccII	CGCG	1 cut(s)	1716
AcoI	YGGCCR	2 cut(s)	565, 1309
AcsI	RAATTY	4 cut(s)	554, 1301, 1644, 1673
AcuI	CTGAAG	1 cut(s)	352
AfaI	GTAC	4 cut(s)	218, 690, 1191, 1286
AfiI	CCNNNNNNNGG	5 cut(s)	154, 313, 351, 395, 1776
AgsI	TTSAA	5 cut(s)	379, 683, 710, 1417, 1760
AjnI	CCWGG	2 cut(s)	324, 509
AjuI	GAANNNNNNNTTGG	2 cut(s)	1593, 1625
AleI	CACNNNNGTG	1 cut(s)	404
AluBI	AGCT	9 cut(s)	382, 782, 890, 911, 969, 1058, 1328, 1607, 1640
AluI	AGCT	9 cut(s)	382, 782, 890, 911, 969, 1058, 1328, 1607, 1640
Alw21I	GWGCWC	4 cut(s)	257, 276, 1060, 1371
Alw26I	GTCTC	2 cut(s)	15, 305
Ama87I	CYCGRG	1 cut(s)	1500
AoxI	GGCC	6 cut(s)	231, 327, 371, 565, 1288, 1309
ApeKI	GCWGC	4 cut(s)	33, 130, 133, 908
ApoI	RAATTY	4 cut(s)	554, 1301, 1644, 1673
ArsI	GACNNNNNNTTYG	2 cut(s)	1231, 1263
Asp700I	GAANNNNTTC	2 cut(s)	1412, 1527
AspLEI	GCGC	3 cut(s)	476, 547, 1716
AspS9I	GGNCC	6 cut(s)	231, 354, 372, 740, 1212, 1351
AsuC2I	CCSGG	2 cut(s)	250, 988
AsuHPI	GGTGA	3 cut(s)	58, 292, 1088
AvaI	CYCGRG	1 cut(s)	1500
AvaII	GGWCC	4 cut(s)	354, 740, 1212, 1351
BaeGI	GKGCMC	1 cut(s)	164
BanI	GGYRCC	2 cut(s)	144, 398
BanII	GRGCYC	3 cut(s)	321, 1060, 1501
BauI	CACGAG	2 cut(s)	783, 1071
BbsI	GAAGAC	4 cut(s)	492, 764, 1151, 1571
Bbv12I	GWGCWC	4 cut(s)	257, 276, 1060, 1371
BbvI	GCAGC	4 cut(s)	45, 142, 145, 920
BccI	CCATC	5 cut(s)	305, 730, 1084, 1652, 1718
BceAI	ACGGC	5 cut(s)	314, 412, 784, 1303, 1788
BciT130I	CCWGG	2 cut(s)	326, 511
BciVI	GTATCC	3 cut(s)	615, 994, 1087
BcnI	CCSGG	2 cut(s)	250, 988
BcoDI	GTCTC	2 cut(s)	15, 305
BfaI	CTAG	2 cut(s)	779, 1329
BfmI	CTRYAG	1 cut(s)	128
BfoI	RGCGCY	1 cut(s)	548
BfuAI	ACCTGC	1 cut(s)	827
BfuI	GTATCC	3 cut(s)	615, 994, 1087
BglII	AGATCT	1 cut(s)	1273
BisI	GCNGC	7 cut(s)	34, 131, 134, 137, 140, 909, 1312
BlsI	GCNGC	7 cut(s)	35, 132, 135, 138, 141, 910, 1313
Bme1390I	CCNGG	4 cut(s)	250, 326, 511, 988
Bme18I	GGWCC	4 cut(s)	354, 740, 1212, 1351
BmeT110I	CYCGRG	1 cut(s)	1500
BmgT120I	GGNCC	6 cut(s)	231, 354, 372, 740, 1212, 1351
BmiI	GGNNCC	6 cut(s)	146, 400, 741, 742, 1030, 1554
BmrFI	CCNGG	4 cut(s)	250, 326, 511, 988
BmrI	ACTGGG	1 cut(s)	163
BmsI	GCATC	3 cut(s)	805, 1092, 1219
BmuI	ACTGGG	1 cut(s)	163
BpiI	GAAGAC	4 cut(s)	492, 764, 1151, 1571
BpmI	CTGGAG	1 cut(s)	734
BpuEI	CTTGAG	1 cut(s)	991
BpuMI	CCSGG	2 cut(s)	250, 988
BsaJI	CCNNGG	5 cut(s)	248, 509, 510, 987, 1770
BsaXI	ACNNNNNCTCC	4 cut(s)	90, 120, 840, 870
Bsc4I	CCNNNNNNNGG	5 cut(s)	154, 313, 351, 395, 1776
Bse1I	ACTGG	6 cut(s)	158, 717, 751, 1193, 1215, 1575
Bse3DI	GCAATG	4 cut(s)	807, 1007, 1485, 1548
BseBI	CCWGG	2 cut(s)	326, 511
BseDI	CCNNGG	5 cut(s)	248, 509, 510, 987, 1770
BseGI	GGATG	4 cut(s)	1083, 1603, 1663, 1729
BseLI	CCNNNNNNNGG	5 cut(s)	154, 313, 351, 395, 1776
BseMI	GCAATG	4 cut(s)	807, 1007, 1485, 1548
BseMII	CTCAG	1 cut(s)	926
BseNI	ACTGG	6 cut(s)	158, 717, 751, 1193, 1215, 1575
BseRI	GAGGAG	8 cut(s)	98, 198, 201, 204, 207, 210, 213, 216
BseSI	GKGCMC	1 cut(s)	164
BseXI	GCAGC	4 cut(s)	45, 142, 145, 920
BseYI	CCCAGC	1 cut(s)	1493
BsgI	GTGCAG	1 cut(s)	678
Bsh1236I	CGCG	1 cut(s)	1716
BshFI	GGCC	6 cut(s)	233, 329, 373, 567, 1290, 1311
BshNI	GGYRCC	2 cut(s)	144, 398
BsiHKAI	GWGCWC	4 cut(s)	257, 276, 1060, 1371
BsiHKCI	CYCGRG	1 cut(s)	1500
BsiSI	CCGG	2 cut(s)	250, 987
BsiWI	CGTACG	1 cut(s)	1284
BslFI	GGGAC	5 cut(s)	59, 231, 371, 527, 726
BslI	CCNNNNNNNGG	5 cut(s)	154, 313, 351, 395, 1776
BsmAI	GTCTC	2 cut(s)	15, 305
BsmBI	CGTCTC	1 cut(s)	305
BsmFI	GGGAC	5 cut(s)	59, 231, 371, 527, 726
BsmI	GAATGC	1 cut(s)	1066
BsnI	GGCC	6 cut(s)	233, 329, 373, 567, 1290, 1311
BsoBI	CYCGRG	1 cut(s)	1500
Bsp1286I	GDGCHC	7 cut(s)	164, 257, 276, 321, 1060, 1371, 1501
Bsp1407I	TGTACA	1 cut(s)	688
Bsp143I	GATC	2 cut(s)	220, 1273
BspANI	GGCC	6 cut(s)	233, 329, 373, 567, 1290, 1311
BspCNI	CTCAG	1 cut(s)	925
BspFNI	CGCG	1 cut(s)	1716
BspLI	GGNNCC	6 cut(s)	146, 400, 741, 742, 1030, 1554
BspMAI	CTGCAG	1 cut(s)	132
BspMI	ACCTGC	1 cut(s)	827
BspQI	GCTCTTC	2 cut(s)	281, 713
BspT107I	GGYRCC	2 cut(s)	144, 398
BsrDI	GCAATG	4 cut(s)	807, 1007, 1485, 1548
BsrGI	TGTACA	1 cut(s)	688
BsrI	ACTGG	6 cut(s)	158, 717, 751, 1193, 1215, 1575
BssECI	CCNNGG	5 cut(s)	248, 509, 510, 987, 1770
BssMI	GATC	2 cut(s)	220, 1273
BssSI	CACGAG	2 cut(s)	783, 1071
Bst2BI	CACGAG	2 cut(s)	783, 1071
Bst2UI	CCWGG	2 cut(s)	326, 511
Bst4CI	ACNGT	4 cut(s)	245, 698, 1033, 1588
Bst6I	CTCTTC	3 cut(s)	18, 281, 713
BstAUI	TGTACA	1 cut(s)	688
BstC8I	GCNNGC	1 cut(s)	164
BstDEI	CTNAG	4 cut(s)	912, 954, 1649, 1750
BstDSI	CCRYGG	1 cut(s)	1770
BstF5I	GGATG	4 cut(s)	1083, 1603, 1663, 1729
BstFNI	CGCG	1 cut(s)	1716
BstH2I	RGCGCY	1 cut(s)	548
BstHHI	GCGC	3 cut(s)	476, 547, 1716
BstKTI	GATC	2 cut(s)	223, 1276
BstMAI	GTCTC	2 cut(s)	15, 305
BstMBI	GATC	2 cut(s)	220, 1273
BstMWI	GCNNNNNNNGC	6 cut(s)	136, 139, 348, 379, 1475, 1490
BstNI	CCWGG	2 cut(s)	326, 511
BstSCI	CCNGG	4 cut(s)	248, 324, 509, 986
BstSFI	CTRYAG	1 cut(s)	128
BstSLI	GKGCMC	1 cut(s)	164
BstUI	CGCG	1 cut(s)	1716
BstV1I	GCAGC	4 cut(s)	45, 142, 145, 920
BstV2I	GAAGAC	4 cut(s)	492, 764, 1151, 1571
BstX2I	RGATCY	1 cut(s)	1273
BstXI	CCANNNNNNTGG	1 cut(s)	265
BstYI	RGATCY	1 cut(s)	1273
BsuI	GTATCC	3 cut(s)	615, 994, 1087
BsuRI	GGCC	6 cut(s)	233, 329, 373, 567, 1290, 1311
BtgI	CCRYGG	1 cut(s)	1770
BtsCI	GGATG	4 cut(s)	1083, 1603, 1663, 1729
BtsIMutI	CAGTG	4 cut(s)	703, 744, 1222, 1582
BveI	ACCTGC	1 cut(s)	827
Cac8I	GCNNGC	1 cut(s)	164
CfoI	GCGC	3 cut(s)	476, 547, 1716
Cfr13I	GGNCC	6 cut(s)	231, 354, 372, 740, 1212, 1351
CseI	GACGC	1 cut(s)	464
Csp6I	GTAC	4 cut(s)	217, 689, 1190, 1285
CviAII	CATG	7 cut(s)	471, 629, 692, 1207, 1420, 1561, 1705
CviQI	GTAC	4 cut(s)	217, 689, 1190, 1285
DdeI	CTNAG	4 cut(s)	912, 954, 1649, 1750
DpnI	GATC	2 cut(s)	222, 1275
DpnII	GATC	2 cut(s)	220, 1273
DrdI	GACNNNNNNGTC	1 cut(s)	649
DseDI	GACNNNNNNGTC	1 cut(s)	649
EaeI	YGGCCR	2 cut(s)	565, 1309
Eam1104I	CTCTTC	3 cut(s)	18, 281, 713
EarI	CTCTTC	3 cut(s)	18, 281, 713
EciI	GGCGGA	3 cut(s)	189, 195, 495
Ecl136II	GAGCTC	1 cut(s)	1058
Eco24I	GRGCYC	3 cut(s)	321, 1060, 1501
Eco47I	GGWCC	4 cut(s)	354, 740, 1212, 1351
Eco53kI	GAGCTC	1 cut(s)	1058
Eco57I	CTGAAG	1 cut(s)	352
Eco88I	CYCGRG	1 cut(s)	1500
EcoICRI	GAGCTC	1 cut(s)	1058
EcoO109I	RGGNCCY	2 cut(s)	372, 740
EcoRII	CCWGG	2 cut(s)	324, 509
EcoT22I	ATGCAT	1 cut(s)	1456
EcoT38I	GRGCYC	3 cut(s)	321, 1060, 1501
Esp3I	CGTCTC	1 cut(s)	305
FaeI	CATG	7 cut(s)	474, 632, 695, 1210, 1423, 1564, 1708
FaqI	GGGAC	5 cut(s)	59, 231, 371, 527, 726
FatI	CATG	7 cut(s)	470, 628, 691, 1206, 1419, 1560, 1704
FauI	CCCGC	2 cut(s)	1709, 1768
FauNDI	CATATG	1 cut(s)	1115
FblI	GTMKAC	2 cut(s)	84, 927
Fnu4HI	GCNGC	7 cut(s)	34, 131, 134, 137, 140, 909, 1312
FokI	GGATG	4 cut(s)	1070, 1610, 1670, 1736
FriOI	GRGCYC	3 cut(s)	321, 1060, 1501
Fsp4HI	GCNGC	7 cut(s)	34, 131, 134, 137, 140, 909, 1312
FspBI	CTAG	2 cut(s)	779, 1329
GlaI	GCGC	3 cut(s)	475, 546, 1715
GluI	GCNGC	7 cut(s)	34, 131, 134, 137, 140, 909, 1312
GsaI	CCCAGC	1 cut(s)	1497
GsuI	CTGGAG	1 cut(s)	734
HaeII	RGCGCY	1 cut(s)	548
HaeIII	GGCC	6 cut(s)	233, 329, 373, 567, 1290, 1311
HapII	CCGG	2 cut(s)	250, 987
HgaI	GACGC	1 cut(s)	464
HhaI	GCGC	3 cut(s)	476, 547, 1716
Hin1II	CATG	7 cut(s)	474, 632, 695, 1210, 1423, 1564, 1708
Hin6I	GCGC	3 cut(s)	474, 545, 1714
HinP1I	GCGC	3 cut(s)	474, 545, 1714
HincII	GTYRAC	1 cut(s)	241
HindII	GTYRAC	1 cut(s)	241
HinfI	GANTC	6 cut(s)	27, 662, 701, 860, 1161, 1169
HpaII	CCGG	2 cut(s)	250, 987
HphI	GGTGA	3 cut(s)	58, 292, 1088
Hpy166II	GTNNAC	9 cut(s)	85, 241, 604, 689, 928, 1190, 1397, 1631, 1739
Hpy188I	TCNGA	5 cut(s)	915, 1048, 1062, 1156, 1618
Hpy188III	TCNNGA	5 cut(s)	1087, 1556, 1682, 1695, 1764
Hpy8I	GTNNAC	9 cut(s)	85, 241, 604, 689, 928, 1190, 1397, 1631, 1739
Hpy99I	CGWCG	1 cut(s)	458
HpyAV	CCTTC	8 cut(s)	54, 370, 412, 460, 677, 952, 1019, 1468
HpyCH4III	ACNGT	4 cut(s)	245, 698, 1033, 1588
HpyCH4IV	ACGT	2 cut(s)	726, 761
HpyF10VI	GCNNNNNNNGC	6 cut(s)	136, 139, 348, 379, 1475, 1490
HpyF3I	CTNAG	4 cut(s)	912, 954, 1649, 1750
HpySE526I	ACGT	2 cut(s)	726, 761
Hsp92II	CATG	7 cut(s)	474, 632, 695, 1210, 1423, 1564, 1708
HspAI	GCGC	3 cut(s)	474, 545, 1714
KflI	GGGWCCC	1 cut(s)	740
Kzo9I	GATC	2 cut(s)	220, 1273
LguI	GCTCTTC	2 cut(s)	281, 713
LmnI	GCTCC	3 cut(s)	260, 1055, 1637
Lsp1109I	GCAGC	4 cut(s)	45, 142, 145, 920
LweI	GCATC	3 cut(s)	805, 1092, 1219
MaeI	CTAG	2 cut(s)	779, 1329
MaeII	ACGT	2 cut(s)	726, 761
MaeIII	GTNAC	3 cut(s)	698, 762, 1237
MalI	GATC	2 cut(s)	222, 1275
MboI	GATC	2 cut(s)	220, 1273
MfeI	CAATTG	1 cut(s)	346
MflI	RGATCY	1 cut(s)	1273
MhlI	GDGCHC	7 cut(s)	164, 257, 276, 321, 1060, 1371, 1501
MluCI	AATT	7 cut(s)	346, 445, 554, 1301, 1433, 1644, 1673
MlyI	GAGTC	2 cut(s)	695, 1155
MmeI	TCCRAC	4 cut(s)	826, 1024, 1233, 1701
Mph1103I	ATGCAT	1 cut(s)	1456
MroXI	GAANNNNTTC	2 cut(s)	1412, 1527
MseI	TTAA	3 cut(s)	423, 431, 827
MslI	CAYNNNNRTG	4 cut(s)	404, 696, 1071, 1088
MspA1I	CMGCKG	1 cut(s)	136
MspI	CCGG	2 cut(s)	250, 987
MspR9I	CCNGG	4 cut(s)	250, 326, 511, 988
MunI	CAATTG	1 cut(s)	346
Mva1269I	GAATGC	1 cut(s)	1066
MvaI	CCWGG	2 cut(s)	326, 511
MvnI	CGCG	1 cut(s)	1716
MwoI	GCNNNNNNNGC	6 cut(s)	136, 139, 348, 379, 1475, 1490
NciI	CCSGG	2 cut(s)	250, 988
NdeI	CATATG	1 cut(s)	1115
NdeII	GATC	2 cut(s)	220, 1273
NlaIII	CATG	7 cut(s)	474, 632, 695, 1210, 1423, 1564, 1708
NlaIV	GGNNCC	6 cut(s)	146, 400, 741, 742, 1030, 1554
NmeAIII	GCCGAG	1 cut(s)	332
NmuCI	GTSAC	2 cut(s)	698, 1237
NsiI	ATGCAT	1 cut(s)	1456
OliI	CACNNNNGTG	1 cut(s)	404
PaeR7I	CTCGAG	1 cut(s)	1500
PasI	CCCWGGG	1 cut(s)	510
PciSI	GCTCTTC	2 cut(s)	281, 713
PcsI	WCGNNNNNNNCGW	1 cut(s)	402
PctI	GAATGC	1 cut(s)	1066
PdmI	GAANNNNTTC	2 cut(s)	1412, 1527
PfeI	GAWTC	4 cut(s)	27, 662, 860, 1169
Pfl23II	CGTACG	1 cut(s)	1284
PkrI	GCNGC	7 cut(s)	35, 132, 135, 138, 141, 910, 1313
PleI	GAGTC	2 cut(s)	695, 1155
PpsI	GAGTC	2 cut(s)	695, 1155
PpuMI	RGGWCCY	1 cut(s)	740
PsiI	TTATAA	1 cut(s)	675
Psp124BI	GAGCTC	1 cut(s)	1060
Psp5II	RGGWCCY	1 cut(s)	740
Psp6I	CCWGG	2 cut(s)	324, 509
PspFI	CCCAGC	1 cut(s)	1493
PspGI	CCWGG	2 cut(s)	324, 509
PspLI	CGTACG	1 cut(s)	1284
PspN4I	GGNNCC	6 cut(s)	146, 400, 741, 742, 1030, 1554
PspPI	GGNCC	6 cut(s)	231, 354, 372, 740, 1212, 1351
PspPPI	RGGWCCY	1 cut(s)	740
PspXI	VCTCGAGB	1 cut(s)	1500
PstI	CTGCAG	1 cut(s)	132
PsuI	RGATCY	1 cut(s)	1273
RsaI	GTAC	4 cut(s)	218, 690, 1191, 1286
RsaNI	GTAC	4 cut(s)	217, 689, 1190, 1285
RseI	CAYNNNNRTG	4 cut(s)	404, 696, 1071, 1088
SacI	GAGCTC	1 cut(s)	1060
SapI	GCTCTTC	2 cut(s)	281, 713
SaqAI	TTAA	3 cut(s)	423, 431, 827
SatI	GCNGC	7 cut(s)	34, 131, 134, 137, 140, 909, 1312
Sau3AI	GATC	2 cut(s)	220, 1273
Sau96I	GGNCC	6 cut(s)	231, 354, 372, 740, 1212, 1351
SchI	GAGTC	2 cut(s)	695, 1155
ScrFI	CCNGG	4 cut(s)	250, 326, 511, 988
SduI	GDGCHC	7 cut(s)	164, 257, 276, 321, 1060, 1371, 1501
SfaNI	GCATC	3 cut(s)	805, 1092, 1219
SfcI	CTRYAG	1 cut(s)	128
Sfr274I	CTCGAG	1 cut(s)	1500
SinI	GGWCC	4 cut(s)	354, 740, 1212, 1351
SlaI	CTCGAG	1 cut(s)	1500
SmiMI	CAYNNNNRTG	4 cut(s)	404, 696, 1071, 1088
SmlI	CTYRAG	2 cut(s)	970, 1500
SmoI	CTYRAG	2 cut(s)	970, 1500
Sse9I	AATT	7 cut(s)	346, 445, 554, 1301, 1433, 1644, 1673
SspI	AATATT	3 cut(s)	867, 1518, 1528
SspMI	CTAG	2 cut(s)	779, 1329
SstI	GAGCTC	1 cut(s)	1060
StyD4I	CCNGG	4 cut(s)	248, 324, 509, 986
TaaI	ACNGT	4 cut(s)	245, 698, 1033, 1588
TaiI	ACGT	2 cut(s)	729, 764
TaqI	TCGA	3 cut(s)	19, 453, 1501
TasI	AATT	7 cut(s)	346, 445, 554, 1301, 1433, 1644, 1673
TatI	WGTACW	1 cut(s)	688
TauI	GCSGC	3 cut(s)	139, 142, 1314
TfiI	GAWTC	4 cut(s)	27, 662, 860, 1169
Tru1I	TTAA	3 cut(s)	423, 431, 827
Tru9I	TTAA	3 cut(s)	423, 431, 827
TscAI	CASTG	4 cut(s)	703, 751, 1222, 1582
TseFI	GTSAC	2 cut(s)	698, 1237
TseI	GCWGC	4 cut(s)	33, 130, 133, 908
Tsp45I	GTSAC	2 cut(s)	698, 1237
TspDTI	ATGAA	8 cut(s)	36, 523, 567, 951, 1102, 1436, 1577, 1614
TspGWI	ACGGA	2 cut(s)	583, 1583
TspRI	CASTG	4 cut(s)	703, 751, 1222, 1582
VpaK11BI	GGWCC	4 cut(s)	354, 740, 1212, 1351
XapI	RAATTY	4 cut(s)	554, 1301, 1644, 1673
XhoI	CTCGAG	1 cut(s)	1500
XmiI	GTMKAC	2 cut(s)	84, 927
XmnI	GAANNNNTTC	2 cut(s)	1412, 1527
XspI	CTAG	2 cut(s)	779, 1329
Zsp2I	ATGCAT	1 cut(s)	1456

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.