AT5G50110

rRNA small subunit methyltransferase G

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008341
Physical Location & Seq
Forward (+)
20373491 .. 20376252
2762 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50110.1

Sequence Viewer

Length: 879 bp
ATGAGATCAATTTGTTCATTTGTTGAAAACACCAAAAAAATGCACTGCGTCTCACATATTCTCTCTCTGGGGCATTTAATCAAACACCTTCCATGCACCAAATCGCACACATTTCTTCGGAGGAGATTTCACTACAAAGCCCTCTCTAATGCCACCGTAAGCACAACAGCTTCATGTTTCAAAACTCTCAGTTCTAGCCAACAGGACCAAATCCATCTCTACGTTGATACACTTCTCCAATGGAACCAGAAAATGAACCTTACAGCAACTAAAGAAGCTGATGAAGTAATGGAGAGGCATATCGAAGATTCTCTTGCGATATTGCCTCCTATAAAGACTTGTTACAACTTACATTCCAGTGATTTGTTTGATCACATCAATTTAATCGATGTTGGAAGCGGGGCTGGTCTTCCTGGGTTGGTTCTAGCCATTGCATGCCCAGATTGGAGAGTTACTCTACTGGAGTCTATAAATAAGCGATGTGTTTTCTTGGAGCACGTTGTGAATGTTACCGGGCTTACAAATGTTACAATCGTTAGGGGCAGAGCAGAGAGTTGTGGGCACGATGTTATGTACAGAGAGAAGTTTGATGTGGCTATTGCAAGAGCTGTCGCGGAGATGAGAGTCTTAGCTGAATATTGTCTTCCTCTGGTTCGGATTGGTGGATTGTTTGTAGCTGCTAAGGGTCATGACCCAAAGGAGGAAGTTCAAAATGCAGAAAATGCTGTTCGCTTGTTGGGTGGTTCAATACTACAAATTAGTCCAGTGGATTCACATAGCCCATACGGACAGCGGACAACGGTTGTTTGTCGTAAAGATCATTCCACCCCACAAAAATATCCACGTGAAGCAGGTACTCCTGCTAAATTACCGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GidB

Protein Analysis

292

Amino Acids

32.49

Weight (kDa)

8.65

Isoelectric Point (pI)

51.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GidB PF02527 67 - 253 1.5e-50 rRNA small subunit methyltransferase G
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 842
AccII CGCG 1 cut(s) 614
AciI CCGC 3 cut(s) 399, 614, 793
AcvI CACGTG 1 cut(s) 845
AdeI CACNNNGTG 1 cut(s) 502
AfaI GTAC 2 cut(s) 575, 856
AfiI CCNNNNNNNGG 1 cut(s) 700
AgsI TTSAA 4 cut(s) 26, 181, 710, 747
AjnI CCWGG 1 cut(s) 412
AluBI AGCT 5 cut(s) 170, 278, 608, 632, 677
AluI AGCT 5 cut(s) 170, 278, 608, 632, 677
Alw21I GWGCWC 1 cut(s) 498
Alw26I GTCTC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 677
AspS9I GGNCC 1 cut(s) 205
AsuC2I CCSGG 1 cut(s) 514
AvaII GGWCC 1 cut(s) 205
BaeGI GKGCMC 1 cut(s) 564
BbrPI CACGTG 1 cut(s) 845
BbsI GAAGAC 2 cut(s) 401, 635
Bbv12I GWGCWC 1 cut(s) 498
BbvI GCAGC 1 cut(s) 664
BccI CCATC 1 cut(s) 222
BciT130I CCWGG 1 cut(s) 414
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 1 cut(s) 514
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 2 cut(s) 195, 425
BfuAI ACCTGC 1 cut(s) 842
BisI GCNGC 1 cut(s) 678
BlsI GCNGC 1 cut(s) 679
Bme1390I CCNGG 2 cut(s) 414, 514
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 1 cut(s) 245
BmrFI CCNGG 2 cut(s) 414, 514
BpiI GAAGAC 2 cut(s) 401, 635
BplI GAGNNNNNCTC 2 cut(s) 439, 471
BpmI CTGGAG 1 cut(s) 482
Bpu10I CCTNAGC 1 cut(s) 681
BpuMI CCSGG 1 cut(s) 514
Bsa29I ATCGAT 1 cut(s) 387
BsaAI YACGTR 1 cut(s) 845
BsaJI CCNNGG 1 cut(s) 413
Bsc4I CCNNNNNNNGG 1 cut(s) 700
Bse1I ACTGG 3 cut(s) 357, 465, 764
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 1 cut(s) 414
BseCI ATCGAT 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 413
BseLI CCNNNNNNNGG 1 cut(s) 700
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 1 cut(s) 202
BseNI ACTGG 3 cut(s) 357, 465, 764
BseRI GAGGAG 1 cut(s) 136
BseSI GKGCMC 1 cut(s) 564
BseXI GCAGC 1 cut(s) 664
Bsh1236I CGCG 1 cut(s) 614
BshVI ATCGAT 1 cut(s) 387
BsiHKAI GWGCWC 1 cut(s) 498
BsiSI CCGG 1 cut(s) 513
BslI CCNNNNNNNGG 1 cut(s) 700
BsmAI GTCTC 1 cut(s) 55
BsmBI CGTCTC 1 cut(s) 55
Bsp1286I GDGCHC 2 cut(s) 498, 564
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 3 cut(s) 5, 370, 817
BspACI CCGC 3 cut(s) 399, 614, 793
BspCNI CTCAG 1 cut(s) 201
BspDI ATCGAT 1 cut(s) 387
BspFNI CGCG 1 cut(s) 614
BspHI TCATGA 1 cut(s) 688
BspLI GGNNCC 1 cut(s) 245
BspMI ACCTGC 1 cut(s) 842
BsrDI GCAATG 1 cut(s) 429
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 3 cut(s) 357, 465, 764
BssECI CCNNGG 1 cut(s) 413
BssMI GATC 3 cut(s) 5, 370, 817
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 3 cut(s) 157, 802, 873
BstAPI GCANNNNNTGC 1 cut(s) 722
BstAUI TGTACA 1 cut(s) 573
BstBAI YACGTR 1 cut(s) 845
BstC8I GCNNGC 1 cut(s) 436
BstDEI CTNAG 3 cut(s) 188, 628, 681
BstFNI CGCG 1 cut(s) 614
BstKTI GATC 3 cut(s) 8, 373, 820
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 3 cut(s) 5, 370, 817
BstMWI GCNNNNNNNGC 1 cut(s) 722
BstNI CCWGG 1 cut(s) 414
BstNSI RCATGY 1 cut(s) 438
BstSCI CCNGG 2 cut(s) 412, 512
BstSLI GKGCMC 1 cut(s) 564
BstUI CGCG 1 cut(s) 614
BstV1I GCAGC 1 cut(s) 664
BstV2I GAAGAC 2 cut(s) 401, 635
Bsu15I ATCGAT 1 cut(s) 387
BsuTUI ATCGAT 1 cut(s) 387
BtgZI GCGATG 1 cut(s) 493
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 3 cut(s) 43, 364, 771
BveI ACCTGC 1 cut(s) 842
Cac8I GCNNGC 1 cut(s) 436
CciI TCATGA 1 cut(s) 688
Cfr13I GGNCC 1 cut(s) 205
ClaI ATCGAT 1 cut(s) 387
CseI GACGC 1 cut(s) 37
Csp6I GTAC 2 cut(s) 574, 855
CviAII CATG 4 cut(s) 93, 174, 435, 689
CviQI GTAC 2 cut(s) 574, 855
DdeI CTNAG 3 cut(s) 188, 628, 681
DpnI GATC 3 cut(s) 7, 372, 819
DpnII GATC 3 cut(s) 5, 370, 817
DraIII CACNNNGTG 1 cut(s) 502
Eco47I GGWCC 1 cut(s) 205
Eco72I CACGTG 1 cut(s) 845
EcoRII CCWGG 1 cut(s) 412
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 4 cut(s) 96, 177, 438, 692
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FatI CATG 4 cut(s) 92, 173, 434, 688
FauI CCCGC 1 cut(s) 392
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 1 cut(s) 678
Fsp4HI GCNGC 1 cut(s) 678
FspBI CTAG 2 cut(s) 195, 425
GluI GCNGC 1 cut(s) 678
GsuI CTGGAG 1 cut(s) 482
HapII CCGG 1 cut(s) 513
HgaI GACGC 1 cut(s) 37
Hin1II CATG 4 cut(s) 96, 177, 438, 692
HinfI GANTC 4 cut(s) 308, 464, 624, 770
HpaII CCGG 1 cut(s) 513
Hpy188I TCNGA 2 cut(s) 120, 657
Hpy188III TCNNGA 1 cut(s) 689
HpyAV CCTTC 1 cut(s) 98
HpyCH4III ACNGT 3 cut(s) 157, 802, 873
HpyCH4IV ACGT 3 cut(s) 222, 498, 844
HpyCH4V TGCA 5 cut(s) 43, 96, 434, 602, 716
HpyF10VI GCNNNNNNNGC 1 cut(s) 722
HpyF3I CTNAG 3 cut(s) 188, 628, 681
HpySE526I ACGT 3 cut(s) 222, 498, 844
Hsp92II CATG 4 cut(s) 96, 177, 438, 692
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 3 cut(s) 5, 370, 817
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 1 cut(s) 664
MaeI CTAG 2 cut(s) 195, 425
MaeII ACGT 3 cut(s) 222, 498, 844
MaeIII GTNAC 4 cut(s) 341, 451, 508, 526
MalI GATC 3 cut(s) 7, 372, 819
MboI GATC 3 cut(s) 5, 370, 817
MboII GAAGA 4 cut(s) 107, 317, 401, 635
MhlI GDGCHC 2 cut(s) 498, 564
MluCI AATT 4 cut(s) 9, 379, 756, 866
MlyI GAGTC 2 cut(s) 473, 633
MmeI TCCRAC 1 cut(s) 373
MnlI CCTC 6 cut(s) 114, 152, 288, 336, 657, 694
MseI TTAA 2 cut(s) 77, 383
MslI CAYNNNNRTG 1 cut(s) 357
MspA1I CMGCKG 1 cut(s) 793
MspI CCGG 1 cut(s) 513
MspR9I CCNGG 2 cut(s) 414, 514
MvaI CCWGG 1 cut(s) 414
MvnI CGCG 1 cut(s) 614
MwoI GCNNNNNNNGC 1 cut(s) 722
NciI CCSGG 1 cut(s) 514
NdeII GATC 3 cut(s) 5, 370, 817
NlaIII CATG 4 cut(s) 96, 177, 438, 692
NlaIV GGNNCC 1 cut(s) 245
NspI RCATGY 1 cut(s) 438
PaeI GCATGC 1 cut(s) 438
PagI TCATGA 1 cut(s) 688
PfeI GAWTC 2 cut(s) 308, 770
PkrI GCNGC 1 cut(s) 679
PleI GAGTC 2 cut(s) 472, 632
PmaCI CACGTG 1 cut(s) 845
PmlI CACGTG 1 cut(s) 845
PpsI GAGTC 2 cut(s) 472, 632
Ppu21I YACGTR 1 cut(s) 845
Psp6I CCWGG 1 cut(s) 412
PspCI CACGTG 1 cut(s) 845
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 1 cut(s) 245
PspPI GGNCC 1 cut(s) 205
RsaI GTAC 2 cut(s) 575, 856
RsaNI GTAC 2 cut(s) 574, 855
RseI CAYNNNNRTG 1 cut(s) 357
SaqAI TTAA 2 cut(s) 77, 383
SatI GCNGC 1 cut(s) 678
Sau3AI GATC 3 cut(s) 5, 370, 817
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 2 cut(s) 473, 633
ScrFI CCNGG 2 cut(s) 414, 514
SduI GDGCHC 2 cut(s) 498, 564
SinI GGWCC 1 cut(s) 205
SmiMI CAYNNNNRTG 1 cut(s) 357
SphI GCATGC 1 cut(s) 438
Sse9I AATT 4 cut(s) 9, 379, 756, 866
SsiI CCGC 3 cut(s) 399, 614, 793
SspI AATATT 1 cut(s) 638
SspMI CTAG 2 cut(s) 195, 425
StyD4I CCNGG 2 cut(s) 412, 512
TaaI ACNGT 3 cut(s) 157, 802, 873
TaiI ACGT 3 cut(s) 225, 501, 847
TaqI TCGA 2 cut(s) 303, 387
TasI AATT 4 cut(s) 9, 379, 756, 866
TatI WGTACW 1 cut(s) 573
TfiI GAWTC 2 cut(s) 308, 770
Tru1I TTAA 2 cut(s) 77, 383
Tru9I TTAA 2 cut(s) 77, 383
TscAI CASTG 3 cut(s) 50, 364, 771
TseI GCWGC 1 cut(s) 677
TspDTI ATGAA 4 cut(s) 6, 162, 269, 297
TspGWI ACGGA 1 cut(s) 801
TspRI CASTG 3 cut(s) 50, 364, 771
VpaK11BI GGWCC 1 cut(s) 205
XceI RCATGY 1 cut(s) 438
XspI CTAG 2 cut(s) 195, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.