Prupe.5G179000_v2.0.a1

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0008341
Physical Location & Seq
Forward (+)
15084129 .. 15086018
1890 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G179000.1

Sequence Viewer

Length: 846 bp
ATGTTAGTTAGCCGTTGCTTAGTTCCATTCTCTCTGGGGACTTTCATCAAACACCTTCCCATATTAAAGCGAACGACTTTCTGTCCGTACAGAATCAGACCCAAAACCATAACCACCACCAGAATTCTCTGCTCCTCTTACTTTGAAACGTTAGATTCGCACCAGAAAGAGCAAATCCATCTCTACGTCGACACTCTTCTCGAATGGAACCAGCGAATGAATTTGACCGCGGTTAAAGAGGTGAATGAGGTAATGGAGAGGCACATTGAGGACTCGCTTGCAATCATACCACCTATAAGAAATTCTTATTTTTCTCATTGTAGCCCTTCTTCGTCAAACAACCTCAGCCTTGTCGATGTGGGCAGCGGTGCGGGTCTTCCCGGATTGGTTTTAGCCATTGCTTGCCCAGGTTGGGAAGTGACCCTCATGGAGTCTATGAATAAACGATGCCTTTTTCTGGAGCATGTTGTCAGTCATATTGGTTTGTCAAATGTTCAAGTTGTAAGAGGAAGAGCAGAGAATTTGGGGCAGGTTCTTTGTTTCAGGGAGAAATTTGATGTGGCAGTAGCCAGAGCTGTTGCAGAGATGAGAGTTTTAGCTGAATACTGTCTACCTCTTGTTTGCGTTGGCGGTTTGTTTGTAGCTGCAAAGGGCCATGATCCGGAGGTGGAAGTTAGGAATGCAGAAAAAGCAATTCAGATATTGGGTGCTTCCGTATTGCAACTGTGCCCAGTGGAATCGCACAGCCCGTACGGACAGAGAACTGCCATTGTATGTTTGAAAACGAGTCCTACCCCGAGGAAGTATCCTCGCGATCCAGGTACTCCAGAGAAAACACCACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GidB

Protein Analysis

282

Amino Acids

31.24

Weight (kDa)

8.47

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 520
AccI GTMKAC 2 cut(s) 189, 610
AccII CGCG 2 cut(s) 230, 813
AccIII TCCGGA 1 cut(s) 661
AciI CCGC 5 cut(s) 228, 230, 366, 371, 630
AclI AACGTT 1 cut(s) 149
AclWI GGATC 2 cut(s) 653, 809
AcsI RAATTY 5 cut(s) 123, 220, 301, 520, 551
AfaI GTAC 3 cut(s) 89, 752, 823
AfiI CCNNNNNNNGG 3 cut(s) 412, 457, 661
AgsI TTSAA 3 cut(s) 146, 497, 781
AhdI GACNNNNNGTC 1 cut(s) 81
AjnI CCWGG 2 cut(s) 406, 817
AluBI AGCT 3 cut(s) 575, 599, 644
AluI AGCT 3 cut(s) 575, 599, 644
AlwI GGATC 2 cut(s) 653, 809
Ama87I CYCGRG 1 cut(s) 796
Aor13HI TCCGGA 1 cut(s) 661
AoxI GGCC 1 cut(s) 652
ApeKI GCWGC 2 cut(s) 363, 644
ApoI RAATTY 5 cut(s) 123, 220, 301, 520, 551
AspS9I GGNCC 1 cut(s) 652
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 1 cut(s) 253
AvaI CYCGRG 1 cut(s) 796
BaeGI GKGCMC 1 cut(s) 731
BarI GAAGNNNNNNTAC 2 cut(s) 313, 345
BbsI GAAGAC 1 cut(s) 368
BbvCI CCTCAGC 1 cut(s) 344
BbvI GCAGC 2 cut(s) 375, 631
BccI CCATC 1 cut(s) 186
BciT130I CCWGG 2 cut(s) 408, 819
BciVI GTATCC 1 cut(s) 816
BcnI CCSGG 1 cut(s) 381
BfuAI ACCTGC 1 cut(s) 520
BfuI GTATCC 1 cut(s) 816
BisI GCNGC 2 cut(s) 364, 645
BlsI GCNGC 2 cut(s) 365, 646
Bme1390I CCNGG 3 cut(s) 381, 408, 819
BmeRI GACNNNNNGTC 1 cut(s) 81
BmeT110I CYCGRG 1 cut(s) 796
BmgT120I GGNCC 1 cut(s) 652
BmiI GGNNCC 1 cut(s) 209
BmrFI CCNGG 3 cut(s) 381, 408, 819
BmrI ACTGGG 1 cut(s) 725
BmsI GCATC 1 cut(s) 437
BmuI ACTGGG 1 cut(s) 725
BpiI GAAGAC 1 cut(s) 368
BpmI CTGGAG 2 cut(s) 479, 810
Bpu10I CCTNAGC 1 cut(s) 344
BpuMI CCSGG 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 228, 406, 797
BsaWI WCCGGW 1 cut(s) 661
Bsc4I CCNNNNNNNGG 3 cut(s) 412, 457, 661
Bse1I ACTGG 1 cut(s) 731
Bse3DI GCAATG 1 cut(s) 396
BseAI TCCGGA 1 cut(s) 661
BseBI CCWGG 2 cut(s) 408, 819
BseDI CCNNGG 3 cut(s) 228, 406, 797
BseLI CCNNNNNNNGG 3 cut(s) 412, 457, 661
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 1 cut(s) 358
BseNI ACTGG 1 cut(s) 731
BseRI GAGGAG 1 cut(s) 124
BseSI GKGCMC 1 cut(s) 731
BseXI GCAGC 2 cut(s) 375, 631
Bsh1236I CGCG 2 cut(s) 230, 813
BshFI GGCC 1 cut(s) 654
BsiHKCI CYCGRG 1 cut(s) 796
BsiSI CCGG 2 cut(s) 381, 662
BsiWI CGTACG 1 cut(s) 750
BslFI GGGAC 1 cut(s) 52
BslI CCNNNNNNNGG 3 cut(s) 412, 457, 661
BsmFI GGGAC 1 cut(s) 52
BsmI GAATGC 1 cut(s) 685
BsnI GGCC 1 cut(s) 654
BsoBI CYCGRG 1 cut(s) 796
Bsp1286I GDGCHC 1 cut(s) 731
Bsp13I TCCGGA 1 cut(s) 661
Bsp143I GATC 2 cut(s) 658, 814
Bsp68I TCGCGA 1 cut(s) 813
BspACI CCGC 5 cut(s) 228, 230, 366, 371, 630
BspANI GGCC 1 cut(s) 654
BspCNI CTCAG 1 cut(s) 357
BspEI TCCGGA 1 cut(s) 661
BspFNI CGCG 2 cut(s) 230, 813
BspLI GGNNCC 1 cut(s) 209
BspMI ACCTGC 1 cut(s) 520
BspPI GGATC 2 cut(s) 653, 809
BspQI GCTCTTC 1 cut(s) 505
BsrDI GCAATG 1 cut(s) 396
BsrI ACTGG 1 cut(s) 731
BssECI CCNNGG 3 cut(s) 228, 406, 797
BssMI GATC 2 cut(s) 658, 814
Bst2UI CCWGG 2 cut(s) 408, 819
Bst4CI ACNGT 3 cut(s) 608, 726, 843
Bst6I CTCTTC 2 cut(s) 201, 505
BstC8I GCNNGC 2 cut(s) 279, 403
BstDEI CTNAG 2 cut(s) 19, 344
BstDSI CCRYGG 1 cut(s) 228
BstFNI CGCG 2 cut(s) 230, 813
BstKTI GATC 2 cut(s) 661, 817
BstMBI GATC 2 cut(s) 658, 814
BstMWI GCNNNNNNNGC 1 cut(s) 689
BstNI CCWGG 2 cut(s) 408, 819
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 3 cut(s) 379, 406, 817
BstSLI GKGCMC 1 cut(s) 731
BstUI CGCG 2 cut(s) 230, 813
BstV1I GCAGC 2 cut(s) 375, 631
BstV2I GAAGAC 1 cut(s) 368
BsuI GTATCC 1 cut(s) 816
BsuRI GGCC 1 cut(s) 654
BtgI CCRYGG 1 cut(s) 228
BtsIMutI CAGTG 2 cut(s) 738, 839
BtuMI TCGCGA 1 cut(s) 813
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 2 cut(s) 279, 403
Cfr13I GGNCC 1 cut(s) 652
Cfr42I CCGCGG 1 cut(s) 231
Csp6I GTAC 3 cut(s) 88, 751, 822
CviAII CATG 3 cut(s) 427, 464, 656
CviQI GTAC 3 cut(s) 88, 751, 822
DdeI CTNAG 2 cut(s) 19, 344
DpnI GATC 2 cut(s) 660, 816
DpnII GATC 2 cut(s) 658, 814
DriI GACNNNNNGTC 1 cut(s) 81
Eam1104I CTCTTC 2 cut(s) 201, 505
Eam1105I GACNNNNNGTC 1 cut(s) 81
EarI CTCTTC 2 cut(s) 201, 505
Eco88I CYCGRG 1 cut(s) 796
EcoRI GAATTC 1 cut(s) 123
EcoRII CCWGG 2 cut(s) 406, 817
FaeI CATG 3 cut(s) 430, 467, 659
FalI AAGNNNNNCTT 2 cut(s) 289, 321
FaqI GGGAC 1 cut(s) 52
FatI CATG 3 cut(s) 426, 463, 655
FauI CCCGC 1 cut(s) 364
FblI GTMKAC 2 cut(s) 189, 610
Fnu4HI GCNGC 2 cut(s) 364, 645
Fsp4HI GCNGC 2 cut(s) 364, 645
GluI GCNGC 2 cut(s) 364, 645
GsuI CTGGAG 2 cut(s) 479, 810
HaeIII GGCC 1 cut(s) 654
HapII CCGG 2 cut(s) 381, 662
Hin1II CATG 3 cut(s) 430, 467, 659
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 6 cut(s) 93, 155, 272, 431, 737, 787
HpaII CCGG 2 cut(s) 381, 662
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 2 cut(s) 190, 611
Hpy188I TCNGA 2 cut(s) 98, 699
Hpy188III TCNNGA 5 cut(s) 200, 458, 662, 812, 827
Hpy8I GTNNAC 2 cut(s) 190, 611
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 2 cut(s) 65, 336
HpyCH4III ACNGT 3 cut(s) 608, 726, 843
HpyCH4IV ACGT 2 cut(s) 149, 186
HpyCH4V TGCA 5 cut(s) 281, 581, 647, 683, 721
HpyF10VI GCNNNNNNNGC 1 cut(s) 689
HpyF3I CTNAG 2 cut(s) 19, 344
HpySE526I ACGT 2 cut(s) 149, 186
Hsp92II CATG 3 cut(s) 430, 467, 659
Kpn2I TCCGGA 1 cut(s) 661
KspI CCGCGG 1 cut(s) 231
Kzo9I GATC 2 cut(s) 658, 814
LguI GCTCTTC 1 cut(s) 505
LmnI GCTCC 2 cut(s) 137, 460
Lsp1109I GCAGC 2 cut(s) 375, 631
LweI GCATC 1 cut(s) 437
MaeII ACGT 2 cut(s) 149, 186
MaeIII GTNAC 1 cut(s) 418
MalI GATC 2 cut(s) 660, 816
MboI GATC 2 cut(s) 658, 814
MboII GAAGA 4 cut(s) 188, 321, 368, 522
MhlI GDGCHC 1 cut(s) 731
MluCI AATT 6 cut(s) 123, 220, 301, 520, 551, 693
MlyI GAGTC 3 cut(s) 266, 440, 796
MroI TCCGGA 1 cut(s) 661
MseI TTAA 2 cut(s) 65, 234
MspA1I CMGCKG 2 cut(s) 230, 366
MspI CCGG 2 cut(s) 381, 662
MspR9I CCNGG 3 cut(s) 381, 408, 819
Mva1269I GAATGC 1 cut(s) 685
MvaI CCWGG 2 cut(s) 408, 819
MvnI CGCG 2 cut(s) 230, 813
MwoI GCNNNNNNNGC 1 cut(s) 689
NciI CCSGG 1 cut(s) 381
NdeII GATC 2 cut(s) 658, 814
NlaIII CATG 3 cut(s) 430, 467, 659
NlaIV GGNNCC 1 cut(s) 209
NmuCI GTSAC 1 cut(s) 418
NruI TCGCGA 1 cut(s) 813
NspI RCATGY 1 cut(s) 467
PciSI GCTCTTC 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 746
PctI GAATGC 1 cut(s) 685
PfeI GAWTC 3 cut(s) 93, 155, 737
Pfl23II CGTACG 1 cut(s) 750
PfoI TCCNGGA 1 cut(s) 379
PkrI GCNGC 2 cut(s) 365, 646
PleI GAGTC 3 cut(s) 266, 439, 795
PpsI GAGTC 3 cut(s) 266, 439, 795
Psp1406I AACGTT 1 cut(s) 149
Psp6I CCWGG 2 cut(s) 406, 817
PspGI CCWGG 2 cut(s) 406, 817
PspLI CGTACG 1 cut(s) 750
PspN4I GGNNCC 1 cut(s) 209
PspPI GGNCC 1 cut(s) 652
RruI TCGCGA 1 cut(s) 813
RsaI GTAC 3 cut(s) 89, 752, 823
RsaNI GTAC 3 cut(s) 88, 751, 822
SacII CCGCGG 1 cut(s) 231
SalI GTCGAC 1 cut(s) 188
SapI GCTCTTC 1 cut(s) 505
SaqAI TTAA 2 cut(s) 65, 234
SatI GCNGC 2 cut(s) 364, 645
Sau3AI GATC 2 cut(s) 658, 814
Sau96I GGNCC 1 cut(s) 652
SchI GAGTC 3 cut(s) 266, 440, 796
ScrFI CCNGG 3 cut(s) 381, 408, 819
SduI GDGCHC 1 cut(s) 731
SfaNI GCATC 1 cut(s) 437
Sfr303I CCGCGG 1 cut(s) 231
SgrBI CCGCGG 1 cut(s) 231
Sse9I AATT 6 cut(s) 123, 220, 301, 520, 551, 693
SsiI CCGC 5 cut(s) 228, 230, 366, 371, 630
StyD4I CCNGG 3 cut(s) 379, 406, 817
TaaI ACNGT 3 cut(s) 608, 726, 843
TaiI ACGT 2 cut(s) 152, 189
TaqI TCGA 3 cut(s) 189, 201, 354
TasI AATT 6 cut(s) 123, 220, 301, 520, 551, 693
TfiI GAWTC 3 cut(s) 93, 155, 737
Tru1I TTAA 2 cut(s) 65, 234
Tru9I TTAA 2 cut(s) 65, 234
TscAI CASTG 2 cut(s) 738, 846
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 2 cut(s) 363, 644
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 3 cut(s) 34, 233, 452
TspGWI ACGGA 3 cut(s) 75, 703, 768
TspRI CASTG 2 cut(s) 738, 846
XapI RAATTY 5 cut(s) 123, 220, 301, 520, 551
XceI RCATGY 1 cut(s) 467
XmiI GTMKAC 2 cut(s) 189, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.