MD14G1186100.v1.1

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008341
Physical Location & Seq
Forward (+)
27841389 .. 27842312
924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1186100.v1.1.491

Sequence Viewer

Length: 651 bp
ATGTTAGTTAGCCGTTGCGCAGTTCAATTCTCTTTTGGGACTTTCTTCAAACACCTTCCGGTATCAAATCCGGCGACGCTCTGTCCGTACAGATTCAGACCCAAAAACCTAACCGCCAACCCAATTCGTAGCTCCTCTTACTTTGAGACGTTAGATTCCTGCCAAAAAGAGCAAGTCCATCTCTATGTCCACACTCTTCTCCAATGGAACCGGAAAATGAATCTGACAGCCGTTAAGGAGGCGGATGAGGTAATGGAGAGGCACATTGAGGACTCGCTCGCAATCATACCGCCTATTAGAAATTCTTATCTCTCTCACTGTAACCCTTCGTCCAAAAACCTCCGCCTTGTAGATGTGGGGAGCGGTGCAGGTCTTCCGGGTTTGATCTTAGCCATTGCTTGGCCAGGTTGGGAAGTGACACTGATGGAGTCTATGAACAAGAGATGCATTTTCTTGGAACACGTTGTCGGTCACATTGGTTTGTCGAATGTTCAAGTTGTAAGAGGAAGAGCAGAGGTATGGTTTCCTGAATACTGTCTTCCTCTGGTTTGCGTTGGCGGTTTGTTTGTTGCTGCAAAGGGCCACGATGTAATCGAAAAGTCTTATCACACTAACCATTCATTTCTGAAATCGTATCCAAAATGTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GidB

Protein Analysis

217

Amino Acids

24.41

Weight (kDa)

8.78

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GidB PF02527 55 - 172 1.4e-33 rRNA small subunit methyltransferase G
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 19
Acc36I ACCTGC 1 cut(s) 359
AccB7I CCANNNNNTGG 1 cut(s) 399
AccBSI CCGCTC 1 cut(s) 363
AciI CCGC 6 cut(s) 114, 242, 290, 343, 363, 558
AcoI YGGCCR 1 cut(s) 401
AcsI RAATTY 1 cut(s) 301
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 1 cut(s) 399
AflIII ACRYGT 1 cut(s) 460
AgsI TTSAA 3 cut(s) 26, 49, 494
AhdI GACNNNNNGTC 1 cut(s) 81
AjnI CCWGG 1 cut(s) 403
AluBI AGCT 1 cut(s) 132
AluI AGCT 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 140
AoxI GGCC 2 cut(s) 401, 580
ApeKI GCWGC 1 cut(s) 572
ApoI RAATTY 1 cut(s) 301
AspLEI GCGC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 580
AsuC2I CCSGG 1 cut(s) 378
BalI TGGCCA 1 cut(s) 403
BbsI GAAGAC 2 cut(s) 365, 530
BbvI GCAGC 1 cut(s) 559
BccI CCATC 2 cut(s) 186, 418
BceAI ACGGC 1 cut(s) 215
BciT130I CCWGG 1 cut(s) 405
BciVI GTATCC 1 cut(s) 645
BcnI CCSGG 1 cut(s) 378
BcoDI GTCTC 1 cut(s) 140
BfuAI ACCTGC 1 cut(s) 359
BfuI GTATCC 1 cut(s) 645
BisI GCNGC 1 cut(s) 573
BlsI GCNGC 1 cut(s) 574
Bme1390I CCNGG 2 cut(s) 378, 405
BmeRI GACNNNNNGTC 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 580
BmiI GGNNCC 1 cut(s) 209
BmrFI CCNGG 2 cut(s) 378, 405
BmsI GCATC 1 cut(s) 434
BpiI GAAGAC 2 cut(s) 365, 530
BpuMI CCSGG 1 cut(s) 378
BsaWI WCCGGW 2 cut(s) 58, 210
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 393
BseBI CCWGG 1 cut(s) 405
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 399
BseMI GCAATG 1 cut(s) 393
BseRI GAGGAG 1 cut(s) 124
BseXI GCAGC 1 cut(s) 559
BsgI GTGCAG 1 cut(s) 387
BshFI GGCC 2 cut(s) 403, 582
BsiSI CCGG 4 cut(s) 59, 71, 211, 377
BslFI GGGAC 1 cut(s) 52
BslI CCNNNNNNNGG 1 cut(s) 399
BsmAI GTCTC 1 cut(s) 140
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 52
BsnI GGCC 2 cut(s) 403, 582
Bsp143I GATC 1 cut(s) 384
BspACI CCGC 6 cut(s) 114, 242, 290, 343, 363, 558
BspANI GGCC 2 cut(s) 403, 582
BspLI GGNNCC 1 cut(s) 209
BspMI ACCTGC 1 cut(s) 359
BspQI GCTCTTC 1 cut(s) 502
BsrBI CCGCTC 1 cut(s) 363
BsrDI GCAATG 1 cut(s) 393
BssMI GATC 1 cut(s) 384
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 2 cut(s) 320, 536
Bst6I CTCTTC 2 cut(s) 201, 502
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 1 cut(s) 388
BstF5I GGATG 1 cut(s) 250
BstHHI GCGC 1 cut(s) 20
BstKTI GATC 1 cut(s) 387
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 1 cut(s) 384
BstNI CCWGG 1 cut(s) 405
BstSCI CCNGG 2 cut(s) 376, 403
BstV1I GCAGC 1 cut(s) 559
BstV2I GAAGAC 2 cut(s) 365, 530
BsuI GTATCC 1 cut(s) 645
BsuRI GGCC 2 cut(s) 403, 582
BtsCI GGATG 1 cut(s) 250
BtsIMutI CAGTG 2 cut(s) 316, 419
BveI ACCTGC 1 cut(s) 359
Cac8I GCNNGC 1 cut(s) 279
CfoI GCGC 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 580
CseI GACGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 88
CviJI RGCY 6 cut(s) 12, 132, 230, 392, 403, 582
CviKI_1 RGCY 6 cut(s) 12, 132, 230, 392, 403, 582
CviQI GTAC 1 cut(s) 88
DdeI CTNAG 1 cut(s) 388
DpnI GATC 1 cut(s) 386
DpnII GATC 1 cut(s) 384
DriI GACNNNNNGTC 1 cut(s) 81
EaeI YGGCCR 1 cut(s) 401
Eam1104I CTCTTC 2 cut(s) 201, 502
Eam1105I GACNNNNNGTC 1 cut(s) 81
EarI CTCTTC 2 cut(s) 201, 502
EciI GGCGGA 2 cut(s) 257, 332
EcoRII CCWGG 1 cut(s) 403
EcoT22I ATGCAT 1 cut(s) 449
Esp3I CGTCTC 1 cut(s) 140
FaiI YATR 4 cut(s) 186, 287, 434, 520
FaqI GGGAC 1 cut(s) 52
Fnu4HI GCNGC 1 cut(s) 573
FokI GGATG 1 cut(s) 257
Fsp4HI GCNGC 1 cut(s) 573
FspI TGCGCA 1 cut(s) 19
GlaI GCGC 1 cut(s) 19
GluI GCNGC 1 cut(s) 573
HaeIII GGCC 2 cut(s) 403, 582
HapII CCGG 4 cut(s) 59, 71, 211, 377
HgaI GACGC 1 cut(s) 85
HhaI GCGC 1 cut(s) 20
Hin6I GCGC 1 cut(s) 18
HinP1I GCGC 1 cut(s) 18
HinfI GANTC 5 cut(s) 93, 155, 220, 272, 428
HpaII CCGG 4 cut(s) 59, 71, 211, 377
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 3 cut(s) 98, 225, 627
Hpy188III TCNNGA 1 cut(s) 527
Hpy8I GTNNAC 1 cut(s) 190
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 2 cut(s) 65, 336
HpyCH4III ACNGT 2 cut(s) 320, 536
HpyCH4IV ACGT 2 cut(s) 149, 462
HpyCH4V TGCA 3 cut(s) 368, 447, 575
HpyF3I CTNAG 1 cut(s) 388
HpySE526I ACGT 2 cut(s) 149, 462
HspAI GCGC 1 cut(s) 18
Kzo9I GATC 1 cut(s) 384
LguI GCTCTTC 1 cut(s) 502
LmnI GCTCC 2 cut(s) 137, 360
Lsp1109I GCAGC 1 cut(s) 559
LweI GCATC 1 cut(s) 434
MaeII ACGT 2 cut(s) 149, 462
MaeIII GTNAC 4 cut(s) 320, 415, 470, 644
MalI GATC 1 cut(s) 386
MbiI CCGCTC 1 cut(s) 363
MboI GATC 1 cut(s) 384
MboII GAAGA 5 cut(s) 37, 188, 365, 519, 530
MlsI TGGCCA 1 cut(s) 403
MluCI AATT 3 cut(s) 26, 123, 301
MluNI TGGCCA 1 cut(s) 403
MlyI GAGTC 2 cut(s) 266, 437
MnlI CCTC 9 cut(s) 145, 232, 241, 252, 262, 350, 497, 508, 552
Mox20I TGGCCA 1 cut(s) 403
Mph1103I ATGCAT 1 cut(s) 449
MscI TGGCCA 1 cut(s) 403
MseI TTAA 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 183
Msp20I TGGCCA 1 cut(s) 403
MspI CCGG 4 cut(s) 59, 71, 211, 377
MspR9I CCNGG 2 cut(s) 378, 405
MvaI CCWGG 1 cut(s) 405
NciI CCSGG 1 cut(s) 378
NdeII GATC 1 cut(s) 384
NlaIV GGNNCC 1 cut(s) 209
NmuCI GTSAC 3 cut(s) 415, 470, 644
NsbI TGCGCA 1 cut(s) 19
NsiI ATGCAT 1 cut(s) 449
PciSI GCTCTTC 1 cut(s) 502
PcsI WCGNNNNNNNCGW 2 cut(s) 83, 591
PfeI GAWTC 3 cut(s) 93, 155, 220
PflMI CCANNNNNTGG 1 cut(s) 399
PkrI GCNGC 1 cut(s) 574
PleI GAGTC 2 cut(s) 266, 436
PpsI GAGTC 2 cut(s) 266, 436
Psp6I CCWGG 1 cut(s) 403
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 1 cut(s) 209
PspPI GGNCC 1 cut(s) 580
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
RseI CAYNNNNRTG 1 cut(s) 183
SapI GCTCTTC 1 cut(s) 502
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 1 cut(s) 573
Sau3AI GATC 1 cut(s) 384
Sau96I GGNCC 1 cut(s) 580
SchI GAGTC 2 cut(s) 266, 437
ScrFI CCNGG 2 cut(s) 378, 405
SfaNI GCATC 1 cut(s) 434
SmiMI CAYNNNNRTG 1 cut(s) 183
Sse9I AATT 3 cut(s) 26, 123, 301
SsiI CCGC 6 cut(s) 114, 242, 290, 343, 363, 558
StyD4I CCNGG 2 cut(s) 376, 403
TaaI ACNGT 2 cut(s) 320, 536
TaiI ACGT 2 cut(s) 152, 465
TaqI TCGA 2 cut(s) 485, 594
TaqII GACCGA 1 cut(s) 458
TasI AATT 3 cut(s) 26, 123, 301
TfiI GAWTC 3 cut(s) 93, 155, 220
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TscAI CASTG 2 cut(s) 323, 426
TseFI GTSAC 3 cut(s) 415, 470, 644
TseI GCWGC 1 cut(s) 572
Tsp45I GTSAC 3 cut(s) 415, 470, 644
TspDTI ATGAA 3 cut(s) 233, 449, 609
TspGWI ACGGA 1 cut(s) 75
TspRI CASTG 2 cut(s) 323, 426
Van91I CCANNNNNTGG 1 cut(s) 399
XapI RAATTY 1 cut(s) 301
Zsp2I ATGCAT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.