Rh7DG027600

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008341
Physical Location & Seq
Forward (+)
2078812 .. 2080647
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG027600.1

Sequence Viewer

Length: 627 bp
ATGAACTTGACCGCAGTCAGAGAAGCGGGTGAGGTAATGGAGAGGCACATTGAAGACTCGCTCGCGGTCATACCGCCCATAACAGACTCTTATCTCTCTCATTGTGGCGCCTCGTGTGGAAATGTCAGCGTTGTGGATGTGGGAAGCGGCGCGGGCCTGCCCGGGTTGGTTTTGGCCATTGCTTGCCCGGAATGGAAAGTGACCCTGATGGAGTCAATGAACAAAAGATGTGTTTTTTTGGAGCATGCTGTTAATCGTGTTGGTTTATCAAATGTGGAGATTGTTAGAGGAAGAGCAGAGAACTTGGGTCAGGATGTTTGCTTCAGGGAGAAATATGATGTAGCAGTGGCCAGAGCGGTTGCGGAGATGAGAATTTTGGCGGAATACTGTCTTCCTCTGGTTCGCGTTGGTGGCTTGTTTGTAGCTGCAAAGGGCCATGATCCTCAGATGGAAATTAGGAATGCAGAAAAAGCAATTCAAATTTTGGGTGCTTCCATATCACAACTGTGCCCAGTGGAATCTCACAGCCCATATGGACAAAGAACGGCCATTGTATGCCTGAAAACCAGTCCCACCCCAAAGAAGTATCCTCGTAATCCAGGTACTCCGGTGAAAGTACCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GidB

Protein Analysis

208

Amino Acids

22.42

Weight (kDa)

7.58

Isoelectric Point (pI)

39.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GidB PF02527 1 - 167 4.3e-45 rRNA small subunit methyltransferase G
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 107
AccBSI CCGCTC 1 cut(s) 356
AccII CGCG 3 cut(s) 65, 152, 405
AciI CCGC 9 cut(s) 12, 26, 65, 74, 147, 152, 356, 362, 380
AclWI GGATC 1 cut(s) 434
AcoI YGGCCR 3 cut(s) 174, 348, 546
AcsI RAATTY 2 cut(s) 372, 480
AcuI CTGAAG 1 cut(s) 307
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 2 cut(s) 604, 618
AgsI TTSAA 2 cut(s) 53, 479
AjnI CCWGG 1 cut(s) 598
AleI CACNNNNGTG 1 cut(s) 505
AluBI AGCT 1 cut(s) 425
AluI AGCT 1 cut(s) 425
AlwI GGATC 1 cut(s) 434
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 5 cut(s) 154, 174, 348, 433, 546
ApeKI GCWGC 1 cut(s) 425
ApoI RAATTY 2 cut(s) 372, 480
AspLEI GCGC 2 cut(s) 110, 152
AspS9I GGNCC 2 cut(s) 154, 433
AsuC2I CCSGG 3 cut(s) 162, 163, 188
AsuHPI GGTGA 2 cut(s) 41, 622
AvaI CYCGRG 1 cut(s) 161
BaeGI GKGCMC 1 cut(s) 512
BaeI ACNNNNGTAYC 2 cut(s) 594, 627
BalI TGGCCA 2 cut(s) 176, 350
BanI GGYRCC 1 cut(s) 107
BauI CACGAG 1 cut(s) 112
BbsI GAAGAC 2 cut(s) 60, 383
BbvI GCAGC 1 cut(s) 412
BccI CCATC 2 cut(s) 202, 442
BceAI ACGGC 1 cut(s) 561
BciT130I CCWGG 1 cut(s) 600
BciVI GTATCC 1 cut(s) 597
BcnI CCSGG 3 cut(s) 162, 163, 188
BfoI RGCGCY 1 cut(s) 111
BfuI GTATCC 1 cut(s) 597
BisI GCNGC 2 cut(s) 148, 426
BlsI GCNGC 2 cut(s) 149, 427
Bme1390I CCNGG 4 cut(s) 162, 163, 188, 600
BmeT110I CYCGRG 1 cut(s) 161
BmgT120I GGNCC 2 cut(s) 154, 433
BmiI GGNNCC 1 cut(s) 109
BmrFI CCNGG 4 cut(s) 162, 163, 188, 600
BmrI ACTGGG 1 cut(s) 506
BmuI ACTGGG 1 cut(s) 506
BoxI GACNNNNGTC 1 cut(s) 14
BpiI GAAGAC 2 cut(s) 60, 383
BpuMI CCSGG 3 cut(s) 162, 163, 188
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 161
BsaWI WCCGGW 1 cut(s) 607
Bse1I ACTGG 2 cut(s) 512, 567
Bse3DI GCAATG 1 cut(s) 177
BseBI CCWGG 1 cut(s) 600
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 2 cut(s) 142, 319
BseMI GCAATG 1 cut(s) 177
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 2 cut(s) 512, 567
BseSI GKGCMC 1 cut(s) 512
BseXI GCAGC 1 cut(s) 412
Bsh1236I CGCG 3 cut(s) 65, 152, 405
BshFI GGCC 5 cut(s) 156, 176, 350, 435, 548
BshNI GGYRCC 1 cut(s) 107
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 3 cut(s) 162, 188, 608
BslFI GGGAC 1 cut(s) 555
BsmFI GGGAC 1 cut(s) 555
BsmI GAATGC 1 cut(s) 466
BsnI GGCC 5 cut(s) 156, 176, 350, 435, 548
BsoBI CYCGRG 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 512
Bsp143I GATC 1 cut(s) 439
BspACI CCGC 9 cut(s) 12, 26, 65, 74, 147, 152, 356, 362, 380
BspANI GGCC 5 cut(s) 156, 176, 350, 435, 548
BspCNI CTCAG 1 cut(s) 457
BspFNI CGCG 3 cut(s) 65, 152, 405
BspLI GGNNCC 1 cut(s) 109
BspPI GGATC 1 cut(s) 434
BspQI GCTCTTC 1 cut(s) 286
BspT107I GGYRCC 1 cut(s) 107
BsrBI CCGCTC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 177
BsrI ACTGG 2 cut(s) 512, 567
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 1 cut(s) 439
BssNI GRCGYC 1 cut(s) 108
BssSI CACGAG 1 cut(s) 112
Bst2BI CACGAG 1 cut(s) 112
Bst2UI CCWGG 1 cut(s) 600
Bst4CI ACNGT 2 cut(s) 389, 507
Bst6I CTCTTC 1 cut(s) 286
BstACI GRCGYC 1 cut(s) 108
BstC8I GCNNGC 5 cut(s) 63, 154, 158, 184, 246
BstDEI CTNAG 1 cut(s) 444
BstF5I GGATG 2 cut(s) 142, 319
BstFNI CGCG 3 cut(s) 65, 152, 405
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 2 cut(s) 110, 152
BstKTI GATC 1 cut(s) 442
BstMBI GATC 1 cut(s) 439
BstMWI GCNNNNNNNGC 3 cut(s) 153, 411, 470
BstNI CCWGG 1 cut(s) 600
BstNSI RCATGY 1 cut(s) 248
BstPAI GACNNNNGTC 1 cut(s) 14
BstSCI CCNGG 4 cut(s) 160, 161, 186, 598
BstSLI GKGCMC 1 cut(s) 512
BstUI CGCG 3 cut(s) 65, 152, 405
BstV1I GCAGC 1 cut(s) 412
BstV2I GAAGAC 2 cut(s) 60, 383
BsuI GTATCC 1 cut(s) 597
BsuRI GGCC 5 cut(s) 156, 176, 350, 435, 548
BtsCI GGATG 2 cut(s) 142, 319
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 2 cut(s) 351, 519
Cac8I GCNNGC 5 cut(s) 63, 154, 158, 184, 246
CfoI GCGC 2 cut(s) 110, 152
Cfr13I GGNCC 2 cut(s) 154, 433
Cfr9I CCCGGG 1 cut(s) 161
Csp6I GTAC 2 cut(s) 603, 617
CviAII CATG 2 cut(s) 245, 437
CviJI RGCY 8 cut(s) 156, 176, 350, 414, 425, 435, 528, 548
CviKI_1 RGCY 8 cut(s) 156, 176, 350, 414, 425, 435, 528, 548
CviQI GTAC 2 cut(s) 603, 617
DdeI CTNAG 1 cut(s) 444
DinI GGCGCC 1 cut(s) 109
DpnI GATC 1 cut(s) 441
DpnII GATC 1 cut(s) 439
EaeI YGGCCR 3 cut(s) 174, 348, 546
Eam1104I CTCTTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 286
EciI GGCGGA 1 cut(s) 395
Eco57I CTGAAG 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 598
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
FaeI CATG 2 cut(s) 248, 440
FaqI GGGAC 1 cut(s) 555
FatI CATG 2 cut(s) 244, 436
FauI CCCGC 2 cut(s) 19, 145
FauNDI CATATG 1 cut(s) 532
Fnu4HI GCNGC 2 cut(s) 148, 426
FokI GGATG 2 cut(s) 149, 326
Fsp4HI GCNGC 2 cut(s) 148, 426
GlaI GCGC 2 cut(s) 109, 151
GluI GCNGC 2 cut(s) 148, 426
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 5 cut(s) 156, 176, 350, 435, 548
HapII CCGG 3 cut(s) 162, 188, 608
HhaI GCGC 2 cut(s) 110, 152
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 2 cut(s) 248, 440
Hin6I GCGC 2 cut(s) 108, 150
HinP1I GCGC 2 cut(s) 108, 150
HinfI GANTC 4 cut(s) 56, 86, 212, 518
HpaII CCGG 3 cut(s) 162, 188, 608
HphI GGTGA 2 cut(s) 41, 622
Hpy188I TCNGA 2 cut(s) 20, 447
Hpy188III TCNNGA 1 cut(s) 311
HpyCH4III ACNGT 2 cut(s) 389, 507
HpyCH4V TGCA 2 cut(s) 428, 464
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 411, 470
HpyF3I CTNAG 1 cut(s) 444
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 2 cut(s) 248, 440
HspAI GCGC 2 cut(s) 108, 150
KasI GGCGCC 1 cut(s) 107
Kzo9I GATC 1 cut(s) 439
LguI GCTCTTC 1 cut(s) 286
LmnI GCTCC 1 cut(s) 241
Lsp1109I GCAGC 1 cut(s) 412
MaeIII GTNAC 1 cut(s) 199
MalI GATC 1 cut(s) 441
MbiI CCGCTC 1 cut(s) 356
MboI GATC 1 cut(s) 439
MboII GAAGA 3 cut(s) 65, 303, 383
MhlI GDGCHC 1 cut(s) 512
MlsI TGGCCA 2 cut(s) 176, 350
MluCI AATT 4 cut(s) 372, 453, 474, 480
MluNI TGGCCA 2 cut(s) 176, 350
Mly113I GGCGCC 1 cut(s) 108
MlyI GAGTC 3 cut(s) 50, 80, 221
MnlI CCTC 7 cut(s) 25, 36, 121, 281, 405, 453, 600
Mox20I TGGCCA 2 cut(s) 176, 350
MscI TGGCCA 2 cut(s) 176, 350
MseI TTAA 1 cut(s) 252
MslI CAYNNNNRTG 1 cut(s) 505
Msp20I TGGCCA 2 cut(s) 176, 350
MspI CCGG 3 cut(s) 162, 188, 608
MspR9I CCNGG 4 cut(s) 162, 163, 188, 600
Mva1269I GAATGC 1 cut(s) 466
MvaI CCWGG 1 cut(s) 600
MvnI CGCG 3 cut(s) 65, 152, 405
MwoI GCNNNNNNNGC 3 cut(s) 153, 411, 470
NarI GGCGCC 1 cut(s) 108
NciI CCSGG 3 cut(s) 162, 163, 188
NdeI CATATG 1 cut(s) 532
NdeII GATC 1 cut(s) 439
NlaIII CATG 2 cut(s) 248, 440
NlaIV GGNNCC 1 cut(s) 109
NmuCI GTSAC 1 cut(s) 199
NspI RCATGY 1 cut(s) 248
OliI CACNNNNGTG 1 cut(s) 505
PaeI GCATGC 1 cut(s) 248
PciSI GCTCTTC 1 cut(s) 286
PctI GAATGC 1 cut(s) 466
PfeI GAWTC 1 cut(s) 518
PkrI GCNGC 2 cut(s) 149, 427
PleI GAGTC 3 cut(s) 50, 80, 220
PluTI GGCGCC 1 cut(s) 111
PpsI GAGTC 3 cut(s) 50, 80, 220
PshAI GACNNNNGTC 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 598
PspGI CCWGG 1 cut(s) 598
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 2 cut(s) 154, 433
RsaI GTAC 2 cut(s) 604, 618
RsaNI GTAC 2 cut(s) 603, 617
RseI CAYNNNNRTG 1 cut(s) 505
SapI GCTCTTC 1 cut(s) 286
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 2 cut(s) 148, 426
Sau3AI GATC 1 cut(s) 439
Sau96I GGNCC 2 cut(s) 154, 433
SchI GAGTC 3 cut(s) 50, 80, 221
ScrFI CCNGG 4 cut(s) 162, 163, 188, 600
SduI GDGCHC 1 cut(s) 512
SetI ASST 3 cut(s) 36, 427, 604
SfoI GGCGCC 1 cut(s) 109
SmaI CCCGGG 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 505
SphI GCATGC 1 cut(s) 248
Sse9I AATT 4 cut(s) 372, 453, 474, 480
SsiI CCGC 9 cut(s) 12, 26, 65, 74, 147, 152, 356, 362, 380
SspDI GGCGCC 1 cut(s) 107
StyD4I CCNGG 4 cut(s) 160, 161, 186, 598
TaaI ACNGT 2 cut(s) 389, 507
TasI AATT 4 cut(s) 372, 453, 474, 480
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 1 cut(s) 518
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 2 cut(s) 351, 519
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 1 cut(s) 425
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 2 cut(s) 17, 233
TspMI CCCGGG 1 cut(s) 161
TspRI CASTG 2 cut(s) 351, 519
XapI RAATTY 2 cut(s) 372, 480
XceI RCATGY 1 cut(s) 248
XmaI CCCGGG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.