Rorug06G0427500

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003458
Physical Location & Seq
Forward (+)
56422465 .. 56424403
1939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0427500.1

Sequence Viewer

Length: 933 bp
ATGGATAATGAAGGTGGTGTTCAACCTAAGCAAGAACGTTCGAGGACTAGGTGGACACCGGCCCTTGACAAGACATTTGCAGACTTGGTTATTAAGCAGATTCAATTGGGGAACAGGCCAAACAATGTTTTTGACAAGAAAACGTGGAATAGTATCCGCGATGAGTTTAACAAGCAAACGGATCTCAACTTCAACAACAATCAATTGAGGAAGCACCTGGATGTTCTAAGGACGCGCTTCTACAATCTCAAGTCGGCTTATGATCAAACTAATGACTATGCTTTGGAAGATCCGTGTTGTATCGAGTTTGATCATCTATGGGCAGACCTAGGGGTACAGCAGCCGAGGCCTGAAACTACTAAAGTGAAGGATTGCCCCATATATGATCAGCTGTGCACAATATTCACAGACTCAGCAGCCGATGGGAAATACGCTGAATCTAGTCATTATGAAGGGTTGGACAAATCTGCAGGAACGGACAATGTAGTTCTGAGTTCCTGTCCTGACAGTGGAGCCCCTCAGTCTGAAAACCCACCATCCTCAAGACCTGTGCAAGGAAATGCATTATTAGCAGAGAAGGTGGCCAAGGTTGTAGTAGAGAGAAAAAGGAAGCGAACATCTGATACACAGTCTTCTGAGGGTCAAAGCAGAAGGGATCAAGAAACATGTGATGCCATGGCAAAAGCCCTACAAGATATGATTGCTGCCTCAAAGCTGAGGACAGCGGCAACAATGCCTAGAGACGACAAGTTTAGCATAACTAATTGTATAAGAGCACTGGATGAGATAAAAGACATTGACGAACAGCTTTACTTTGCTGCAGTTGACCTGTTTGATAACCCTAATTTAAGGGAGACATTCATCTCTCTAAAAGGTGATCAAATACGGTTGAGATGGTTGCAAGGGAAGTGCAGTAAAGCTGCCTACAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GidB

Protein Analysis

310

Amino Acids

35.14

Weight (kDa)

5.79

Isoelectric Point (pI)

55.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_3 PF12776 17 - 86 1.8e-13 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 159, 235
AciI CCGC 2 cut(s) 157, 725
AclI AACGTT 1 cut(s) 37
AclWI GGATC 3 cut(s) 189, 284, 663
AcoI YGGCCR 1 cut(s) 582
AfaI GTAC 1 cut(s) 336
AfiI CCNNNNNNNGG 2 cut(s) 509, 554
AflIII ACRYGT 1 cut(s) 665
AgsI TTSAA 3 cut(s) 23, 104, 193
AjnI CCWGG 1 cut(s) 216
AluBI AGCT 4 cut(s) 391, 715, 808, 920
AluI AGCT 4 cut(s) 391, 715, 808, 920
Alw21I GWGCWC 2 cut(s) 398, 778
Alw26I GTCTC 2 cut(s) 735, 848
Alw44I GTGCAC 1 cut(s) 394
AlwI GGATC 3 cut(s) 189, 284, 663
AoxI GGCC 4 cut(s) 60, 116, 347, 582
ApaLI GTGCAC 1 cut(s) 394
ApeKI GCWGC 5 cut(s) 340, 416, 704, 818, 920
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspA2I CCTAGG 1 cut(s) 328
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 1 cut(s) 887
AvrII CCTAGG 1 cut(s) 328
BaeGI GKGCMC 1 cut(s) 398
BalI TGGCCA 1 cut(s) 584
BanII GRGCYC 1 cut(s) 517
BbsI GAAGAC 1 cut(s) 624
Bbv12I GWGCWC 2 cut(s) 398, 778
BbvCI CCTCAGC 1 cut(s) 716
BbvI GCAGC 5 cut(s) 352, 428, 691, 805, 907
BccI CCATC 3 cut(s) 416, 544, 888
BciT130I CCWGG 1 cut(s) 218
BciVI GTATCC 1 cut(s) 164
BclI TGATCA 4 cut(s) 262, 310, 385, 877
BcoDI GTCTC 2 cut(s) 735, 848
BfaI CTAG 4 cut(s) 48, 329, 441, 738
BfmI CTRYAG 3 cut(s) 468, 819, 925
BfuI GTATCC 1 cut(s) 164
BisI GCNGC 6 cut(s) 341, 417, 705, 726, 819, 921
BlnI CCTAGG 1 cut(s) 328
BlsI GCNGC 6 cut(s) 342, 418, 706, 727, 820, 922
Bme1390I CCNGG 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 514
BmrFI CCNGG 1 cut(s) 218
BmsI GCATC 1 cut(s) 661
BpiI GAAGAC 1 cut(s) 624
Bpu10I CCTNAGC 2 cut(s) 27, 716
BpuEI CTTGAG 2 cut(s) 233, 526
BsaJI CCNNGG 4 cut(s) 328, 344, 585, 675
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 554
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 1 cut(s) 783
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 4 cut(s) 328, 344, 585, 675
BseGI GGATG 3 cut(s) 226, 536, 787
BseLI CCNNNNNNNGG 2 cut(s) 509, 554
BseMII CTCAG 5 cut(s) 426, 482, 533, 627, 707
BseNI ACTGG 1 cut(s) 783
BseSI GKGCMC 1 cut(s) 398
BseXI GCAGC 5 cut(s) 352, 428, 691, 805, 907
BsgI GTGCAG 1 cut(s) 931
Bsh1236I CGCG 2 cut(s) 159, 235
BshFI GGCC 4 cut(s) 62, 118, 349, 584
BsiHKAI GWGCWC 2 cut(s) 398, 778
BsiSI CCGG 1 cut(s) 59
BslI CCNNNNNNNGG 2 cut(s) 509, 554
BsmAI GTCTC 2 cut(s) 735, 848
BsmBI CGTCTC 1 cut(s) 735
BsnI GGCC 4 cut(s) 62, 118, 349, 584
Bsp1286I GDGCHC 3 cut(s) 398, 517, 778
Bsp143I GATC 7 cut(s) 181, 262, 289, 310, 385, 655, 877
Bsp19I CCATGG 1 cut(s) 675
BspACI CCGC 2 cut(s) 157, 725
BspANI GGCC 4 cut(s) 62, 118, 349, 584
BspCNI CTCAG 5 cut(s) 425, 483, 532, 628, 708
BspFNI CGCG 2 cut(s) 159, 235
BspLI GGNNCC 1 cut(s) 514
BspMAI CTGCAG 2 cut(s) 472, 823
BspPI GGATC 3 cut(s) 189, 284, 663
BsrFI RCCGGY 1 cut(s) 58
BsrI ACTGG 1 cut(s) 783
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 4 cut(s) 328, 344, 585, 675
BssMI GATC 7 cut(s) 181, 262, 289, 310, 385, 655, 877
BssT1I CCWWGG 3 cut(s) 328, 585, 675
Bst2UI CCWGG 1 cut(s) 218
Bst4CI ACNGT 4 cut(s) 509, 630, 888, 929
BstDEI CTNAG 7 cut(s) 27, 227, 412, 491, 519, 636, 716
BstDSI CCRYGG 1 cut(s) 675
BstENI CCTNNNNNAGG 1 cut(s) 552
BstF5I GGATG 3 cut(s) 226, 536, 787
BstFNI CGCG 2 cut(s) 159, 235
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 7 cut(s) 184, 265, 292, 313, 388, 658, 880
BstMAI GTCTC 2 cut(s) 735, 848
BstMBI GATC 7 cut(s) 181, 262, 289, 310, 385, 655, 877
BstMWI GCNNNNNNNGC 2 cut(s) 346, 569
BstNI CCWGG 1 cut(s) 218
BstNSI RCATGY 1 cut(s) 669
BstSCI CCNGG 1 cut(s) 216
BstSFI CTRYAG 3 cut(s) 468, 819, 925
BstSLI GKGCMC 1 cut(s) 398
BstUI CGCG 2 cut(s) 159, 235
BstV1I GCAGC 5 cut(s) 352, 428, 691, 805, 907
BstV2I GAAGAC 1 cut(s) 624
BstX2I RGATCY 2 cut(s) 181, 289
BstYI RGATCY 2 cut(s) 181, 289
BsuI GTATCC 1 cut(s) 164
BsuRI GGCC 4 cut(s) 62, 118, 349, 584
BtgI CCRYGG 1 cut(s) 675
BtgZI GCGATG 1 cut(s) 174
BtsCI GGATG 3 cut(s) 226, 536, 787
BtsIMutI CAGTG 2 cut(s) 514, 776
CfoI GCGC 1 cut(s) 237
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 1 cut(s) 61
CseI GACGC 1 cut(s) 241
Csp6I GTAC 1 cut(s) 335
CviAII CATG 2 cut(s) 666, 676
CviQI GTAC 1 cut(s) 335
DdeI CTNAG 7 cut(s) 27, 227, 412, 491, 519, 636, 716
DpnI GATC 7 cut(s) 183, 264, 291, 312, 387, 657, 879
DpnII GATC 7 cut(s) 181, 262, 289, 310, 385, 655, 877
EaeI YGGCCR 1 cut(s) 582
Eco130I CCWWGG 3 cut(s) 328, 585, 675
Eco147I AGGCCT 1 cut(s) 349
Eco24I GRGCYC 1 cut(s) 517
EcoNI CCTNNNNNAGG 1 cut(s) 552
EcoRII CCWGG 1 cut(s) 216
EcoT14I CCWWGG 3 cut(s) 328, 585, 675
EcoT22I ATGCAT 1 cut(s) 565
EcoT38I GRGCYC 1 cut(s) 517
ErhI CCWWGG 3 cut(s) 328, 585, 675
Esp3I CGTCTC 1 cut(s) 735
FaeI CATG 2 cut(s) 669, 679
FatI CATG 2 cut(s) 665, 675
FbaI TGATCA 4 cut(s) 262, 310, 385, 877
Fnu4HI GCNGC 6 cut(s) 341, 417, 705, 726, 819, 921
FokI GGATG 3 cut(s) 233, 523, 794
FriOI GRGCYC 1 cut(s) 517
Fsp4HI GCNGC 6 cut(s) 341, 417, 705, 726, 819, 921
FspBI CTAG 4 cut(s) 48, 329, 441, 738
GlaI GCGC 1 cut(s) 236
GluI GCNGC 6 cut(s) 341, 417, 705, 726, 819, 921
HaeIII GGCC 4 cut(s) 62, 118, 349, 584
HapII CCGG 1 cut(s) 59
HgaI GACGC 1 cut(s) 241
HhaI GCGC 1 cut(s) 237
Hin1II CATG 2 cut(s) 669, 679
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HinfI GANTC 3 cut(s) 100, 410, 437
HpaII CCGG 1 cut(s) 59
HphI GGTGA 1 cut(s) 887
Hpy166II GTNNAC 3 cut(s) 54, 396, 826
Hpy188I TCNGA 4 cut(s) 492, 526, 622, 637
Hpy188III TCNNGA 3 cut(s) 503, 543, 659
Hpy8I GTNNAC 3 cut(s) 54, 396, 826
HpyAV CCTTC 5 cut(s) 5, 361, 446, 571, 645
HpyCH4III ACNGT 4 cut(s) 509, 630, 888, 929
HpyCH4IV ACGT 2 cut(s) 37, 143
HpyCH4V TGCA 8 cut(s) 80, 396, 470, 553, 563, 821, 901, 912
HpyF10VI GCNNNNNNNGC 2 cut(s) 346, 569
HpyF3I CTNAG 7 cut(s) 27, 227, 412, 491, 519, 636, 716
HpySE526I ACGT 2 cut(s) 37, 143
Hsp92II CATG 2 cut(s) 669, 679
HspAI GCGC 1 cut(s) 235
Ksp22I TGATCA 4 cut(s) 262, 310, 385, 877
Kzo9I GATC 7 cut(s) 181, 262, 289, 310, 385, 655, 877
LmnI GCTCC 1 cut(s) 512
Lsp1109I GCAGC 5 cut(s) 352, 428, 691, 805, 907
LweI GCATC 1 cut(s) 661
MaeI CTAG 4 cut(s) 48, 329, 441, 738
MaeII ACGT 2 cut(s) 37, 143
MalI GATC 7 cut(s) 183, 264, 291, 312, 387, 657, 879
MboI GATC 7 cut(s) 181, 262, 289, 310, 385, 655, 877
MboII GAAGA 2 cut(s) 299, 624
MfeI CAATTG 2 cut(s) 104, 203
MflI RGATCY 2 cut(s) 181, 289
MhlI GDGCHC 3 cut(s) 398, 517, 778
MlsI TGGCCA 1 cut(s) 584
MluCI AATT 4 cut(s) 104, 203, 763, 844
MluNI TGGCCA 1 cut(s) 584
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 438
MnlI CCTC 8 cut(s) 36, 201, 339, 528, 550, 631, 711, 718
Mox20I TGGCCA 1 cut(s) 584
Mph1103I ATGCAT 1 cut(s) 565
MscI TGGCCA 1 cut(s) 584
MseI TTAA 3 cut(s) 93, 168, 848
MslI CAYNNNNRTG 1 cut(s) 219
Msp20I TGGCCA 1 cut(s) 584
MspA1I CMGCKG 2 cut(s) 391, 725
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 218
MunI CAATTG 2 cut(s) 104, 203
MvaI CCWGG 1 cut(s) 218
MvnI CGCG 2 cut(s) 159, 235
MwoI GCNNNNNNNGC 2 cut(s) 346, 569
NcoI CCATGG 1 cut(s) 675
NdeII GATC 7 cut(s) 181, 262, 289, 310, 385, 655, 877
NlaIII CATG 2 cut(s) 669, 679
NlaIV GGNNCC 1 cut(s) 514
NmeAIII GCCGAG 1 cut(s) 369
NsiI ATGCAT 1 cut(s) 565
NspI RCATGY 1 cut(s) 669
PceI AGGCCT 1 cut(s) 349
PciI ACATGT 1 cut(s) 665
PfeI GAWTC 2 cut(s) 100, 437
PkrI GCNGC 6 cut(s) 342, 418, 706, 727, 820, 922
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PscI ACATGT 1 cut(s) 665
Psp1406I AACGTT 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 216
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 514
PspPI GGNCC 1 cut(s) 61
PsrI GAACNNNNNNTAC 2 cut(s) 607, 639
PstI CTGCAG 2 cut(s) 472, 823
PsuI RGATCY 2 cut(s) 181, 289
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
RseI CAYNNNNRTG 1 cut(s) 219
SaqAI TTAA 3 cut(s) 93, 168, 848
SatI GCNGC 6 cut(s) 341, 417, 705, 726, 819, 921
Sau3AI GATC 7 cut(s) 181, 262, 289, 310, 385, 655, 877
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 218
SduI GDGCHC 3 cut(s) 398, 517, 778
SfaNI GCATC 1 cut(s) 661
SfcI CTRYAG 3 cut(s) 468, 819, 925
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 2 cut(s) 248, 541
SmoI CTYRAG 2 cut(s) 248, 541
Sse9I AATT 4 cut(s) 104, 203, 763, 844
SseBI AGGCCT 1 cut(s) 349
SsiI CCGC 2 cut(s) 157, 725
SspI AATATT 1 cut(s) 402
SspMI CTAG 4 cut(s) 48, 329, 441, 738
StuI AGGCCT 1 cut(s) 349
StyD4I CCNGG 1 cut(s) 216
StyI CCWWGG 3 cut(s) 328, 585, 675
TaaI ACNGT 4 cut(s) 509, 630, 888, 929
TaiI ACGT 2 cut(s) 40, 146
TaqI TCGA 2 cut(s) 41, 303
TasI AATT 4 cut(s) 104, 203, 763, 844
TauI GCSGC 1 cut(s) 728
TfiI GAWTC 2 cut(s) 100, 437
Tru1I TTAA 3 cut(s) 93, 168, 848
Tru9I TTAA 3 cut(s) 93, 168, 848
TscAI CASTG 2 cut(s) 514, 783
TseI GCWGC 5 cut(s) 340, 416, 704, 818, 920
TspDTI ATGAA 3 cut(s) 24, 465, 850
TspGWI ACGGA 3 cut(s) 194, 282, 491
TspRI CASTG 2 cut(s) 514, 783
VneI GTGCAC 1 cut(s) 394
XagI CCTNNNNNAGG 1 cut(s) 552
XceI RCATGY 1 cut(s) 669
XmaJI CCTAGG 1 cut(s) 328
XspI CTAG 4 cut(s) 48, 329, 441, 738
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.