Rw7G002160

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008341
Physical Location & Seq
Forward (+)
1812177 .. 1813815
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002160.1

Sequence Viewer

Length: 876 bp
ATGTTGTTGTTGGTTAGCCGTTGCTCTCTAGTCCCATTCTCTCTGGGGACTTTCTTCAAACACCTTCCGCGCCCAAAACCCAGTCCCATCTTTCCCTACACAATCACACCCAAAGCCCTAACCACCGCCACAATCCACTGCTCCTCTCACTTCGAAGCCTTGGACTCCCGCCAAAAACACCAAATCCATCTCTACGTCGACACCCTTCTCGAATGGAACCAGAGAATGAACTTGACCGCGGTCAGAGAAGCGGGTGAGGTAATGGAGAGGCACATTGAAGACTCGCTGGCGATCATACCGCCCATAACAGACTCTTATCTCTCTCATTGTGGCGCCTCGTGTGGGAATGTCAGCGTTGTGGATGTGGGAAGCGGCGCGGGCCTGCCCGGGTTGGTTTTGGCCATTGCTTGCCCGGAATGGAAAGTGACCCTGATGGAGTCAATGAACAAAAGATGTGTTTTTTTGGAGCATGCTGTTAATCATGTTGGTTTATCAAATGTGGAGATTGTTAGAGGAAGAGCAGAGTGTTTCAAATTTTGTTATAGGACAGAACTTGGGTCAGGATGTTGCTTCAGGGAGAAGTATGATGTAGCAGTGGCCAGAGCGGTTGCGGAGATGAGAATTTTGGCGGAATACTGTCTTCCTCTGGTTCGCGTTGGTGGCTTGTTTGTAGCTGCAAAGGGCCATGATCCTCAGATGGAAATTAGGAATGCAGAAAAAGCAATTCAAATTTTGGGTGCTTCCATATCACAACTGTGCCCAGTGGAATCTCACAGCCCATATGGACAAAGAACGGCCATTGTATGTCTGAAAACCAGTCCCACCCCAAAGAAGTATCCTCGTGATCCAGGTACCCCGGTGAAAGTACCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GidB

Protein Analysis

291

Amino Acids

32.04

Weight (kDa)

8.54

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GidB PF02527 58 - 250 5e-49 rRNA small subunit methyltransferase G
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 851
AccB1I GGYRCC 2 cut(s) 332, 851
AccBSI CCGCTC 1 cut(s) 605
AccI GTMKAC 1 cut(s) 198
AccII CGCG 4 cut(s) 70, 239, 377, 654
AclWI GGATC 2 cut(s) 683, 839
AcoI YGGCCR 3 cut(s) 399, 597, 795
AcsI RAATTY 3 cut(s) 533, 621, 729
AcuI CTGAAG 1 cut(s) 556
AcyI GRCGYC 1 cut(s) 333
AfaI GTAC 2 cut(s) 853, 867
AfiI CCNNNNNNNGG 1 cut(s) 342
AgsI TTSAA 4 cut(s) 58, 278, 532, 728
AjnI CCWGG 1 cut(s) 847
AleI CACNNNNGTG 1 cut(s) 754
AluBI AGCT 1 cut(s) 674
AluI AGCT 1 cut(s) 674
AlwI GGATC 2 cut(s) 683, 839
Ama87I CYCGRG 1 cut(s) 386
AoxI GGCC 5 cut(s) 379, 399, 597, 682, 795
ApeKI GCWGC 1 cut(s) 674
ApoI RAATTY 3 cut(s) 533, 621, 729
Asp718I GGTACC 1 cut(s) 851
AspLEI GCGC 3 cut(s) 72, 335, 377
AspS9I GGNCC 2 cut(s) 379, 682
AsuC2I CCSGG 4 cut(s) 387, 388, 413, 857
AsuHPI GGTGA 2 cut(s) 266, 871
AsuII TTCGAA 1 cut(s) 153
AvaI CYCGRG 1 cut(s) 386
BaeGI GKGCMC 1 cut(s) 761
BaeI ACNNNNGTAYC 2 cut(s) 843, 876
BalI TGGCCA 2 cut(s) 401, 599
BanI GGYRCC 2 cut(s) 332, 851
BauI CACGAG 2 cut(s) 337, 840
BbsI GAAGAC 2 cut(s) 285, 632
BbvI GCAGC 1 cut(s) 661
BccI CCATC 4 cut(s) 95, 195, 427, 691
BceAI ACGGC 2 cut(s) 3, 810
BciT130I CCWGG 1 cut(s) 849
BciVI GTATCC 1 cut(s) 846
BcnI CCSGG 4 cut(s) 387, 388, 413, 857
BfaI CTAG 1 cut(s) 29
BfoI RGCGCY 1 cut(s) 336
BfuI GTATCC 1 cut(s) 846
BisI GCNGC 2 cut(s) 373, 675
BlsI GCNGC 2 cut(s) 374, 676
Bme1390I CCNGG 5 cut(s) 387, 388, 413, 849, 857
BmeT110I CYCGRG 1 cut(s) 386
BmgT120I GGNCC 2 cut(s) 379, 682
BmiI GGNNCC 3 cut(s) 218, 334, 853
BmrFI CCNGG 5 cut(s) 387, 388, 413, 849, 857
BmrI ACTGGG 2 cut(s) 75, 755
BmuI ACTGGG 2 cut(s) 75, 755
BoxI GACNNNNGTC 1 cut(s) 239
BpiI GAAGAC 2 cut(s) 285, 632
Bpu14I TTCGAA 1 cut(s) 153
BpuMI CCSGG 4 cut(s) 387, 388, 413, 857
BsaHI GRCGYC 1 cut(s) 333
BsaJI CCNNGG 4 cut(s) 159, 237, 386, 855
Bsc4I CCNNNNNNNGG 1 cut(s) 342
Bse1I ACTGG 3 cut(s) 81, 761, 816
Bse3DI GCAATG 1 cut(s) 402
BseBI CCWGG 1 cut(s) 849
BseDI CCNNGG 4 cut(s) 159, 237, 386, 855
BseGI GGATG 2 cut(s) 367, 569
BseLI CCNNNNNNNGG 1 cut(s) 342
BseMI GCAATG 1 cut(s) 402
BseMII CTCAG 1 cut(s) 707
BseNI ACTGG 3 cut(s) 81, 761, 816
BseRI GAGGAG 1 cut(s) 133
BseSI GKGCMC 1 cut(s) 761
BseXI GCAGC 1 cut(s) 661
Bsh1236I CGCG 4 cut(s) 70, 239, 377, 654
BshFI GGCC 5 cut(s) 381, 401, 599, 684, 797
BshNI GGYRCC 2 cut(s) 332, 851
BsiHKCI CYCGRG 1 cut(s) 386
BsiSI CCGG 3 cut(s) 387, 413, 857
BslFI GGGAC 4 cut(s) 17, 61, 69, 804
BslI CCNNNNNNNGG 1 cut(s) 342
BsmFI GGGAC 4 cut(s) 17, 61, 69, 804
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 5 cut(s) 381, 401, 599, 684, 797
BsoBI CYCGRG 1 cut(s) 386
Bsp119I TTCGAA 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 761
Bsp143I GATC 3 cut(s) 291, 688, 844
BspANI GGCC 5 cut(s) 381, 401, 599, 684, 797
BspCNI CTCAG 1 cut(s) 706
BspFNI CGCG 4 cut(s) 70, 239, 377, 654
BspLI GGNNCC 3 cut(s) 218, 334, 853
BspPI GGATC 2 cut(s) 683, 839
BspQI GCTCTTC 1 cut(s) 511
BspT104I TTCGAA 1 cut(s) 153
BspT107I GGYRCC 2 cut(s) 332, 851
BsrBI CCGCTC 1 cut(s) 605
BsrDI GCAATG 1 cut(s) 402
BsrI ACTGG 3 cut(s) 81, 761, 816
BssECI CCNNGG 4 cut(s) 159, 237, 386, 855
BssMI GATC 3 cut(s) 291, 688, 844
BssNI GRCGYC 1 cut(s) 333
BssSI CACGAG 2 cut(s) 337, 840
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 2 cut(s) 337, 840
Bst2UI CCWGG 1 cut(s) 849
Bst4CI ACNGT 2 cut(s) 638, 756
Bst6I CTCTTC 1 cut(s) 511
BstACI GRCGYC 1 cut(s) 333
BstBI TTCGAA 1 cut(s) 153
BstC8I GCNNGC 5 cut(s) 288, 379, 383, 409, 471
BstDEI CTNAG 1 cut(s) 693
BstDSI CCRYGG 1 cut(s) 237
BstF5I GGATG 2 cut(s) 367, 569
BstFNI CGCG 4 cut(s) 70, 239, 377, 654
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 3 cut(s) 72, 335, 377
BstKTI GATC 3 cut(s) 294, 691, 847
BstMBI GATC 3 cut(s) 291, 688, 844
BstMWI GCNNNNNNNGC 3 cut(s) 378, 660, 719
BstNI CCWGG 1 cut(s) 849
BstNSI RCATGY 1 cut(s) 473
BstPAI GACNNNNGTC 1 cut(s) 239
BstSCI CCNGG 5 cut(s) 385, 386, 411, 847, 855
BstSLI GKGCMC 1 cut(s) 761
BstUI CGCG 4 cut(s) 70, 239, 377, 654
BstV1I GCAGC 1 cut(s) 661
BstV2I GAAGAC 2 cut(s) 285, 632
BsuI GTATCC 1 cut(s) 846
BsuRI GGCC 5 cut(s) 381, 401, 599, 684, 797
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 2 cut(s) 367, 569
BtsI GCAGTG 2 cut(s) 136, 600
BtsIMutI CAGTG 3 cut(s) 136, 600, 768
Cac8I GCNNGC 5 cut(s) 288, 379, 383, 409, 471
CfoI GCGC 3 cut(s) 72, 335, 377
Cfr13I GGNCC 2 cut(s) 379, 682
Cfr42I CCGCGG 1 cut(s) 240
Cfr9I CCCGGG 1 cut(s) 386
Csp6I GTAC 2 cut(s) 852, 866
CviAII CATG 3 cut(s) 470, 482, 686
CviQI GTAC 2 cut(s) 852, 866
DdeI CTNAG 1 cut(s) 693
DinI GGCGCC 1 cut(s) 334
DpnI GATC 3 cut(s) 293, 690, 846
DpnII GATC 3 cut(s) 291, 688, 844
EaeI YGGCCR 3 cut(s) 399, 597, 795
Eam1104I CTCTTC 1 cut(s) 511
EarI CTCTTC 1 cut(s) 511
EciI GGCGGA 1 cut(s) 644
Eco130I CCWWGG 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 556
Eco88I CYCGRG 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 847
EcoT14I CCWWGG 1 cut(s) 159
EgeI GGCGCC 1 cut(s) 334
EheI GGCGCC 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 3 cut(s) 473, 485, 689
FaqI GGGAC 4 cut(s) 17, 61, 69, 804
FatI CATG 3 cut(s) 469, 481, 685
FauI CCCGC 3 cut(s) 176, 244, 370
FauNDI CATATG 1 cut(s) 781
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 2 cut(s) 373, 675
FokI GGATG 2 cut(s) 374, 576
Fsp4HI GCNGC 2 cut(s) 373, 675
FspBI CTAG 1 cut(s) 29
GlaI GCGC 3 cut(s) 71, 334, 376
GluI GCNGC 2 cut(s) 373, 675
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 5 cut(s) 381, 401, 599, 684, 797
HapII CCGG 3 cut(s) 387, 413, 857
HhaI GCGC 3 cut(s) 72, 335, 377
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 3 cut(s) 473, 485, 689
Hin6I GCGC 3 cut(s) 70, 333, 375
HinP1I GCGC 3 cut(s) 70, 333, 375
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 5 cut(s) 164, 281, 311, 437, 767
HpaII CCGG 3 cut(s) 387, 413, 857
HphI GGTGA 2 cut(s) 266, 871
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 3 cut(s) 245, 696, 810
Hpy188III TCNNGA 3 cut(s) 209, 561, 842
Hpy8I GTNNAC 1 cut(s) 199
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 2 cut(s) 74, 215
HpyCH4III ACNGT 2 cut(s) 638, 756
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 2 cut(s) 677, 713
HpyF10VI GCNNNNNNNGC 3 cut(s) 378, 660, 719
HpyF3I CTNAG 1 cut(s) 693
HpySE526I ACGT 1 cut(s) 195
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 3 cut(s) 473, 485, 689
HspAI GCGC 3 cut(s) 70, 333, 375
KasI GGCGCC 1 cut(s) 332
KpnI GGTACC 1 cut(s) 855
KspI CCGCGG 1 cut(s) 240
Kzo9I GATC 3 cut(s) 291, 688, 844
LguI GCTCTTC 1 cut(s) 511
LmnI GCTCC 2 cut(s) 146, 466
Lsp1109I GCAGC 1 cut(s) 661
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 424
MalI GATC 3 cut(s) 293, 690, 846
MbiI CCGCTC 1 cut(s) 605
MboI GATC 3 cut(s) 291, 688, 844
MboII GAAGA 4 cut(s) 46, 290, 528, 632
MhlI GDGCHC 1 cut(s) 761
MlsI TGGCCA 2 cut(s) 401, 599
MluCI AATT 5 cut(s) 533, 621, 702, 723, 729
MluNI TGGCCA 2 cut(s) 401, 599
Mly113I GGCGCC 1 cut(s) 333
MlyI GAGTC 4 cut(s) 158, 275, 305, 446
MnlI CCTC 8 cut(s) 154, 250, 261, 346, 506, 654, 702, 849
Mox20I TGGCCA 2 cut(s) 401, 599
MscI TGGCCA 2 cut(s) 401, 599
MseI TTAA 1 cut(s) 477
MslI CAYNNNNRTG 1 cut(s) 754
Msp20I TGGCCA 2 cut(s) 401, 599
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 3 cut(s) 387, 413, 857
MspR9I CCNGG 5 cut(s) 387, 388, 413, 849, 857
Mva1269I GAATGC 1 cut(s) 715
MvaI CCWGG 1 cut(s) 849
MvnI CGCG 4 cut(s) 70, 239, 377, 654
MwoI GCNNNNNNNGC 3 cut(s) 378, 660, 719
NarI GGCGCC 1 cut(s) 333
NciI CCSGG 4 cut(s) 387, 388, 413, 857
NdeI CATATG 1 cut(s) 781
NdeII GATC 3 cut(s) 291, 688, 844
NlaIII CATG 3 cut(s) 473, 485, 689
NlaIV GGNNCC 3 cut(s) 218, 334, 853
NmuCI GTSAC 1 cut(s) 424
NspI RCATGY 1 cut(s) 473
NspV TTCGAA 1 cut(s) 153
OliI CACNNNNGTG 1 cut(s) 754
PaeI GCATGC 1 cut(s) 473
PciSI GCTCTTC 1 cut(s) 511
PctI GAATGC 1 cut(s) 715
PfeI GAWTC 1 cut(s) 767
PkrI GCNGC 2 cut(s) 374, 676
PleI GAGTC 4 cut(s) 158, 275, 305, 445
PluTI GGCGCC 1 cut(s) 336
PpsI GAGTC 4 cut(s) 158, 275, 305, 445
PshAI GACNNNNGTC 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 847
PspGI CCWGG 1 cut(s) 847
PspN4I GGNNCC 3 cut(s) 218, 334, 853
PspPI GGNCC 2 cut(s) 379, 682
RsaI GTAC 2 cut(s) 853, 867
RsaNI GTAC 2 cut(s) 852, 866
RseI CAYNNNNRTG 1 cut(s) 754
SacII CCGCGG 1 cut(s) 240
SalI GTCGAC 1 cut(s) 197
SapI GCTCTTC 1 cut(s) 511
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 2 cut(s) 373, 675
Sau3AI GATC 3 cut(s) 291, 688, 844
Sau96I GGNCC 2 cut(s) 379, 682
SchI GAGTC 4 cut(s) 158, 275, 305, 446
ScrFI CCNGG 5 cut(s) 387, 388, 413, 849, 857
SduI GDGCHC 1 cut(s) 761
SetI ASST 5 cut(s) 66, 198, 261, 676, 853
SfoI GGCGCC 1 cut(s) 334
Sfr303I CCGCGG 1 cut(s) 240
SfuI TTCGAA 1 cut(s) 153
SgrBI CCGCGG 1 cut(s) 240
SmaI CCCGGG 1 cut(s) 388
SmiMI CAYNNNNRTG 1 cut(s) 754
SphI GCATGC 1 cut(s) 473
Sse9I AATT 5 cut(s) 533, 621, 702, 723, 729
SspDI GGCGCC 1 cut(s) 332
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 5 cut(s) 385, 386, 411, 847, 855
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 638, 756
TaiI ACGT 1 cut(s) 198
TaqI TCGA 3 cut(s) 153, 198, 210
TasI AATT 5 cut(s) 533, 621, 702, 723, 729
TauI GCSGC 1 cut(s) 375
TfiI GAWTC 1 cut(s) 767
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 3 cut(s) 143, 600, 768
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 1 cut(s) 674
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 242, 458
TspMI CCCGGG 1 cut(s) 386
TspRI CASTG 3 cut(s) 143, 600, 768
XapI RAATTY 3 cut(s) 533, 621, 729
XceI RCATGY 1 cut(s) 473
XmaI CCCGGG 1 cut(s) 386
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.