Rh7BG026000

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0008341
Physical Location & Seq
Forward (+)
1849473 .. 1851896
2424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG026000.1

Sequence Viewer

Length: 801 bp
ATGTTGTTGTTGGTTAGCCGTTGCGCACTAGTCCCATTCTCTCTGGGGACTTTCTTCAAACACCTTCCGCGCCCAAAACCCAGTCCCATCTTTCCTTACACAATCACACCCAAAACCCTAACCACCACCACAATCCACTCCTCCTCTCACTTCGAAGCCTTGGATTCCCGCCAAAAACACCAAATCCATCTCTACGTCGACACCCTTCTCGAATGGAACCAGAGAATGAACTTGACCGCGGTCAGAGAAGCGGGTGAGGTAATGGAGAGGCACATTGAAGACTCGCTCGCGATCATACCGCCCATAACAGACTCTTATCTCTCTCATTGTGGCGCCTCGTGTGGAAATGTCAGCGTTGTGGATGTGGGAAGCGGCGCGGGCCTGCCCGGGTTGGTTTTGGCCATTGCGTGCCCGGAATGGAAAGTGACCCTGATGGAGTCAATGAACAAAAGATGTGTTTTTTTGGAGCATGCTGTTAATCATGTTGGTTTATCAAATGTGGAGATTGTTAGAGGAAGAGCAGAGAACTTGGGTCAGGATGTTTGCTTCAGGGAGAAATATGATGTAGCGGTAGCCAGAGCGGTTGCAGAAATGAGAATTTTGGCGGAATACTGTCTTCCTCTGGTTCGCGTTGGTGGCTTGTTTGTAGCTGCAAAGGGCCATGATCCTCAAATGGAAGTTAGGAATGCAGAAAAAGCAATTCAAATTTTGGGTGCTTCCATATCACAACTGTGCCCAGGTATGTCTTATTTCCAATTTGAAAGAAAATCAACCGCTCATGTGTATGATGGACATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GidB

Protein Analysis

266

Amino Acids

29.43

Weight (kDa)

6.89

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GidB PF02527 58 - 240 3.6e-51 rRNA small subunit methyltransferase G
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 25
AccB1I GGYRCC 1 cut(s) 332
AccBSI CCGCTC 2 cut(s) 581, 776
AccI GTMKAC 1 cut(s) 198
AccII CGCG 5 cut(s) 70, 239, 290, 377, 630
AclWI GGATC 1 cut(s) 659
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 2 cut(s) 597, 705
AcuI CTGAAG 1 cut(s) 532
AcyI GRCGYC 1 cut(s) 333
AgsI TTSAA 4 cut(s) 58, 278, 704, 761
AhlI ACTAGT 1 cut(s) 28
AjnI CCWGG 1 cut(s) 736
AleI CACNNNNGTG 1 cut(s) 730
AluBI AGCT 1 cut(s) 650
AluI AGCT 1 cut(s) 650
AlwI GGATC 1 cut(s) 659
Ama87I CYCGRG 1 cut(s) 386
AoxI GGCC 3 cut(s) 379, 399, 658
ApeKI GCWGC 1 cut(s) 650
ApoI RAATTY 2 cut(s) 597, 705
AspLEI GCGC 4 cut(s) 26, 72, 335, 377
AspS9I GGNCC 2 cut(s) 379, 658
AsuC2I CCSGG 3 cut(s) 387, 388, 413
AsuHPI GGTGA 1 cut(s) 266
AsuII TTCGAA 1 cut(s) 153
AvaI CYCGRG 1 cut(s) 386
BaeGI GKGCMC 2 cut(s) 413, 737
BalI TGGCCA 1 cut(s) 401
BanI GGYRCC 1 cut(s) 332
BauI CACGAG 1 cut(s) 337
BbsI GAAGAC 2 cut(s) 285, 608
BbvI GCAGC 1 cut(s) 637
BccI CCATC 4 cut(s) 95, 195, 427, 782
BceAI ACGGC 1 cut(s) 3
BciT130I CCWGG 1 cut(s) 738
BcnI CCSGG 3 cut(s) 387, 388, 413
BcuI ACTAGT 1 cut(s) 28
BfaI CTAG 1 cut(s) 29
BfoI RGCGCY 1 cut(s) 336
BisI GCNGC 2 cut(s) 373, 651
BlsI GCNGC 2 cut(s) 374, 652
Bme1390I CCNGG 4 cut(s) 387, 388, 413, 738
BmeT110I CYCGRG 1 cut(s) 386
BmgT120I GGNCC 2 cut(s) 379, 658
BmiI GGNNCC 2 cut(s) 218, 334
BmrFI CCNGG 4 cut(s) 387, 388, 413, 738
BmrI ACTGGG 1 cut(s) 75
BmuI ACTGGG 1 cut(s) 75
BoxI GACNNNNGTC 1 cut(s) 239
BpiI GAAGAC 2 cut(s) 285, 608
Bpu14I TTCGAA 1 cut(s) 153
BpuMI CCSGG 3 cut(s) 387, 388, 413
BsaBI GATNNNNATC 1 cut(s) 792
BsaHI GRCGYC 1 cut(s) 333
BsaJI CCNNGG 4 cut(s) 159, 237, 386, 736
Bse1I ACTGG 1 cut(s) 81
Bse3DI GCAATG 1 cut(s) 402
Bse8I GATNNNNATC 1 cut(s) 792
BseBI CCWGG 1 cut(s) 738
BseDI CCNNGG 4 cut(s) 159, 237, 386, 736
BseGI GGATG 2 cut(s) 367, 544
BseJI GATNNNNATC 1 cut(s) 792
BseMI GCAATG 1 cut(s) 402
BseNI ACTGG 1 cut(s) 81
BseRI GAGGAG 2 cut(s) 130, 133
BseSI GKGCMC 2 cut(s) 413, 737
BseXI GCAGC 1 cut(s) 637
Bsh1236I CGCG 5 cut(s) 70, 239, 290, 377, 630
BshFI GGCC 3 cut(s) 381, 401, 660
BshNI GGYRCC 1 cut(s) 332
BsiHKCI CYCGRG 1 cut(s) 386
BsiSI CCGG 2 cut(s) 387, 413
BslFI GGGAC 3 cut(s) 17, 61, 69
BsmFI GGGAC 3 cut(s) 17, 61, 69
BsmI GAATGC 1 cut(s) 691
BsnI GGCC 3 cut(s) 381, 401, 660
BsoBI CYCGRG 1 cut(s) 386
Bsp119I TTCGAA 1 cut(s) 153
Bsp1286I GDGCHC 2 cut(s) 413, 737
Bsp143I GATC 2 cut(s) 291, 664
Bsp68I TCGCGA 1 cut(s) 290
BspANI GGCC 3 cut(s) 381, 401, 660
BspFNI CGCG 5 cut(s) 70, 239, 290, 377, 630
BspLI GGNNCC 2 cut(s) 218, 334
BspPI GGATC 1 cut(s) 659
BspQI GCTCTTC 1 cut(s) 511
BspT104I TTCGAA 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 332
BsrBI CCGCTC 2 cut(s) 581, 776
BsrDI GCAATG 1 cut(s) 402
BsrI ACTGG 1 cut(s) 81
BssECI CCNNGG 4 cut(s) 159, 237, 386, 736
BssMI GATC 2 cut(s) 291, 664
BssNI GRCGYC 1 cut(s) 333
BssSI CACGAG 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 738
Bst4CI ACNGT 2 cut(s) 614, 732
Bst6I CTCTTC 1 cut(s) 511
BstACI GRCGYC 1 cut(s) 333
BstBI TTCGAA 1 cut(s) 153
BstC8I GCNNGC 5 cut(s) 288, 379, 383, 409, 471
BstDSI CCRYGG 1 cut(s) 237
BstF5I GGATG 2 cut(s) 367, 544
BstFNI CGCG 5 cut(s) 70, 239, 290, 377, 630
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 4 cut(s) 26, 72, 335, 377
BstKTI GATC 2 cut(s) 294, 667
BstMBI GATC 2 cut(s) 291, 664
BstMWI GCNNNNNNNGC 3 cut(s) 378, 636, 695
BstNI CCWGG 1 cut(s) 738
BstNSI RCATGY 1 cut(s) 473
BstPAI GACNNNNGTC 1 cut(s) 239
BstSCI CCNGG 4 cut(s) 385, 386, 411, 736
BstSLI GKGCMC 2 cut(s) 413, 737
BstUI CGCG 5 cut(s) 70, 239, 290, 377, 630
BstV1I GCAGC 1 cut(s) 637
BstV2I GAAGAC 2 cut(s) 285, 608
BsuRI GGCC 3 cut(s) 381, 401, 660
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 2 cut(s) 367, 544
BtuMI TCGCGA 1 cut(s) 290
Cac8I GCNNGC 5 cut(s) 288, 379, 383, 409, 471
CfoI GCGC 4 cut(s) 26, 72, 335, 377
Cfr13I GGNCC 2 cut(s) 379, 658
Cfr42I CCGCGG 1 cut(s) 240
Cfr9I CCCGGG 1 cut(s) 386
CviAII CATG 4 cut(s) 470, 482, 662, 779
CviJI RGCY 8 cut(s) 18, 158, 381, 401, 575, 639, 650, 660
CviKI_1 RGCY 8 cut(s) 18, 158, 381, 401, 575, 639, 650, 660
DinI GGCGCC 1 cut(s) 334
DpnI GATC 2 cut(s) 293, 666
DpnII GATC 2 cut(s) 291, 664
EaeI YGGCCR 1 cut(s) 399
Eam1104I CTCTTC 1 cut(s) 511
EarI CTCTTC 1 cut(s) 511
EciI GGCGGA 1 cut(s) 620
Eco130I CCWWGG 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 532
Eco88I CYCGRG 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 736
EcoT14I CCWWGG 1 cut(s) 159
EgeI GGCGCC 1 cut(s) 334
EheI GGCGCC 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 4 cut(s) 473, 485, 665, 782
FaqI GGGAC 3 cut(s) 17, 61, 69
FatI CATG 4 cut(s) 469, 481, 661, 778
FauI CCCGC 3 cut(s) 176, 244, 370
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 2 cut(s) 373, 651
FokI GGATG 2 cut(s) 374, 551
Fsp4HI GCNGC 2 cut(s) 373, 651
FspBI CTAG 1 cut(s) 29
FspI TGCGCA 1 cut(s) 25
GlaI GCGC 4 cut(s) 25, 71, 334, 376
GluI GCNGC 2 cut(s) 373, 651
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 3 cut(s) 381, 401, 660
HapII CCGG 2 cut(s) 387, 413
HhaI GCGC 4 cut(s) 26, 72, 335, 377
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 4 cut(s) 473, 485, 665, 782
Hin6I GCGC 4 cut(s) 24, 70, 333, 375
HinP1I GCGC 4 cut(s) 24, 70, 333, 375
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 4 cut(s) 164, 281, 311, 437
HpaII CCGG 2 cut(s) 387, 413
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 1 cut(s) 245
Hpy188III TCNNGA 3 cut(s) 209, 289, 536
Hpy8I GTNNAC 1 cut(s) 199
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 2 cut(s) 74, 215
HpyCH4III ACNGT 2 cut(s) 614, 732
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 3 cut(s) 587, 653, 689
HpyF10VI GCNNNNNNNGC 3 cut(s) 378, 636, 695
HpySE526I ACGT 1 cut(s) 195
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 4 cut(s) 473, 485, 665, 782
HspAI GCGC 4 cut(s) 24, 70, 333, 375
KasI GGCGCC 1 cut(s) 332
KspI CCGCGG 1 cut(s) 240
Kzo9I GATC 2 cut(s) 291, 664
LguI GCTCTTC 1 cut(s) 511
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 1 cut(s) 637
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 424
MalI GATC 2 cut(s) 293, 666
MbiI CCGCTC 2 cut(s) 581, 776
MboI GATC 2 cut(s) 291, 664
MboII GAAGA 4 cut(s) 46, 290, 528, 608
MhlI GDGCHC 2 cut(s) 413, 737
MlsI TGGCCA 1 cut(s) 401
MluCI AATT 4 cut(s) 597, 699, 705, 755
MluNI TGGCCA 1 cut(s) 401
Mly113I GGCGCC 1 cut(s) 333
MlyI GAGTC 3 cut(s) 275, 305, 446
MnlI CCTC 8 cut(s) 151, 154, 250, 261, 346, 506, 630, 678
Mox20I TGGCCA 1 cut(s) 401
MscI TGGCCA 1 cut(s) 401
MseI TTAA 1 cut(s) 477
MslI CAYNNNNRTG 2 cut(s) 730, 783
Msp20I TGGCCA 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 2 cut(s) 387, 413
MspR9I CCNGG 4 cut(s) 387, 388, 413, 738
Mva1269I GAATGC 1 cut(s) 691
MvaI CCWGG 1 cut(s) 738
MvnI CGCG 5 cut(s) 70, 239, 290, 377, 630
MwoI GCNNNNNNNGC 3 cut(s) 378, 636, 695
NarI GGCGCC 1 cut(s) 333
NciI CCSGG 3 cut(s) 387, 388, 413
NdeII GATC 2 cut(s) 291, 664
NlaIII CATG 4 cut(s) 473, 485, 665, 782
NlaIV GGNNCC 2 cut(s) 218, 334
NmuCI GTSAC 1 cut(s) 424
NruI TCGCGA 1 cut(s) 290
NsbI TGCGCA 1 cut(s) 25
NspI RCATGY 1 cut(s) 473
NspV TTCGAA 1 cut(s) 153
OliI CACNNNNGTG 1 cut(s) 730
PaeI GCATGC 1 cut(s) 473
PciSI GCTCTTC 1 cut(s) 511
PctI GAATGC 1 cut(s) 691
PfeI GAWTC 1 cut(s) 164
PkrI GCNGC 2 cut(s) 374, 652
PleI GAGTC 3 cut(s) 275, 305, 445
PluTI GGCGCC 1 cut(s) 336
PpsI GAGTC 3 cut(s) 275, 305, 445
PshAI GACNNNNGTC 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 736
PspGI CCWGG 1 cut(s) 736
PspN4I GGNNCC 2 cut(s) 218, 334
PspPI GGNCC 2 cut(s) 379, 658
RruI TCGCGA 1 cut(s) 290
RseI CAYNNNNRTG 2 cut(s) 730, 783
SacII CCGCGG 1 cut(s) 240
SalI GTCGAC 1 cut(s) 197
SapI GCTCTTC 1 cut(s) 511
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 2 cut(s) 373, 651
Sau3AI GATC 2 cut(s) 291, 664
Sau96I GGNCC 2 cut(s) 379, 658
SchI GAGTC 3 cut(s) 275, 305, 446
ScrFI CCNGG 4 cut(s) 387, 388, 413, 738
SduI GDGCHC 2 cut(s) 413, 737
SetI ASST 5 cut(s) 66, 198, 261, 652, 742
SfoI GGCGCC 1 cut(s) 334
Sfr303I CCGCGG 1 cut(s) 240
SfuI TTCGAA 1 cut(s) 153
SgrBI CCGCGG 1 cut(s) 240
SmaI CCCGGG 1 cut(s) 388
SmiMI CAYNNNNRTG 2 cut(s) 730, 783
SpeI ACTAGT 1 cut(s) 28
SphI GCATGC 1 cut(s) 473
Sse9I AATT 4 cut(s) 597, 699, 705, 755
SspDI GGCGCC 1 cut(s) 332
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 4 cut(s) 385, 386, 411, 736
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 614, 732
TaiI ACGT 1 cut(s) 198
TaqI TCGA 3 cut(s) 153, 198, 210
TasI AATT 4 cut(s) 597, 699, 705, 755
TauI GCSGC 1 cut(s) 375
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 1 cut(s) 650
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 242, 458
TspMI CCCGGG 1 cut(s) 386
XapI RAATTY 2 cut(s) 597, 705
XceI RCATGY 1 cut(s) 473
XmaI CCCGGG 1 cut(s) 386
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.