MD06G1181000.v1.1

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008341
Physical Location & Seq
Forward (+)
32049818 .. 32051544
1727 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1181000.v1.1.491

Sequence Viewer

Length: 843 bp
ATGTTAGTTAGCCGTTGCGCAGTTCCATTCTCTTTGGGGACTTTCTTCAAACACCTTCCGGCATCGAATCCGACGACGCTCTGTCCGTACAGATTTAGAAGCAAAACCCTAAGCGCCACCGCAATTCGTAGCTTCTCTTACTTTGAGACGTTGGATTCCCGCCAAAAAGAGCAAGTCCATCTCTACGTCGACACTCTCCTCCAATGGAACCAGAAAATGAATTTGACAGCTGTTAAGGAGGCGGATGAGGTAATGGAGAGGCACATTGAGGACTCGCTCGCAATCATACCCCCTATCAGAAACTCATATTTCTCTCACTGTAAAGTTTCGTCCGAAAGTCTCAGCCTTGTCGATGTGGGGAGCGGTGCAGGTCTTCCGGGGTTGATCTTAGCCATTGCTTGCCCAGGTTGGGAAGTGACCCTCATGGAGTCTATGAACAAGCGATGCGTTTTCTTGGAGCATGTTGTCGGTCACATTGGTTTGTCGAATGTTCAAGTTATAAGAGGAAGAGCAGAGAATTTGGGGCAGGCTCTTTGTTTCAGGGAAAAATTTGATGTAGCGGTAGCCAGAGCAGTTGCAGAAATGAGAGTTTTAGCTGAATACTGTCTTCCTCTGGTTTGCGTTGGCGGTTTGTTTGTTGCTGCAAAGGGTCACGATCCTCAGGAGGAAGTTAGGAATGCAGAAAAAGCAATTCAGATTTTGGGTGCTTCCGTATTGCAACAATGCACAGTGGAATCGCACAGCCCGTATGGACAAAGAACTGCCATCGTGTGTTTGAAAACTAGTCCAACGCCAAGGAAGTATCCTCGCGATCCAGGTACTCCAGAGAAAATACCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GidB

Protein Analysis

281

Amino Acids

30.94

Weight (kDa)

8.31

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GidB PF02527 55 - 241 3.1e-50 rRNA small subunit methyltransferase G
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
Acc16I TGCGCA 1 cut(s) 19
Acc36I ACCTGC 1 cut(s) 359
AccBSI CCGCTC 1 cut(s) 363
AccI GTMKAC 1 cut(s) 189
AccII CGCG 1 cut(s) 810
AciI CCGC 6 cut(s) 120, 160, 242, 363, 560, 627
AclWI GGATC 2 cut(s) 650, 806
AcsI RAATTY 3 cut(s) 220, 517, 548
AfaI GTAC 2 cut(s) 89, 820
AfiI CCNNNNNNNGG 1 cut(s) 409
AgsI TTSAA 3 cut(s) 49, 494, 778
AhdI GACNNNNNGTC 1 cut(s) 81
AhlI ACTAGT 1 cut(s) 782
AjnI CCWGG 2 cut(s) 403, 814
AluBI AGCT 3 cut(s) 132, 230, 596
AluI AGCT 3 cut(s) 132, 230, 596
Alw26I GTCTC 2 cut(s) 140, 344
AlwI GGATC 2 cut(s) 650, 806
ApeKI GCWGC 1 cut(s) 641
ApoI RAATTY 3 cut(s) 220, 517, 548
AspLEI GCGC 2 cut(s) 20, 116
AsuC2I CCSGG 1 cut(s) 378
AxyI CCTNAGG 1 cut(s) 660
BbsI GAAGAC 2 cut(s) 365, 599
BbvI GCAGC 1 cut(s) 628
BccI CCATC 2 cut(s) 186, 773
BciT130I CCWGG 2 cut(s) 405, 816
BciVI GTATCC 1 cut(s) 813
BcnI CCSGG 1 cut(s) 378
BcoDI GTCTC 2 cut(s) 140, 344
BcuI ACTAGT 1 cut(s) 782
BfaI CTAG 1 cut(s) 783
BfoI RGCGCY 1 cut(s) 117
BfuAI ACCTGC 1 cut(s) 359
BfuI GTATCC 1 cut(s) 813
BisI GCNGC 1 cut(s) 642
BlsI GCNGC 1 cut(s) 643
Bme1390I CCNGG 3 cut(s) 378, 405, 816
BmeRI GACNNNNNGTC 1 cut(s) 81
BmiI GGNNCC 1 cut(s) 209
BmrFI CCNGG 3 cut(s) 378, 405, 816
BmsI GCATC 2 cut(s) 71, 434
BpiI GAAGAC 2 cut(s) 365, 599
BpmI CTGGAG 1 cut(s) 807
Bpu10I CCTNAGC 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 378
BsaJI CCNNGG 3 cut(s) 377, 403, 794
Bsc4I CCNNNNNNNGG 1 cut(s) 409
Bse21I CCTNAGG 1 cut(s) 660
Bse3DI GCAATG 1 cut(s) 393
BseBI CCWGG 2 cut(s) 405, 816
BseDI CCNNGG 3 cut(s) 377, 403, 794
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 409
BseMI GCAATG 1 cut(s) 393
BseMII CTCAG 2 cut(s) 355, 674
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 1 cut(s) 628
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 810
BsiSI CCGG 2 cut(s) 59, 377
BslFI GGGAC 1 cut(s) 52
BslI CCNNNNNNNGG 1 cut(s) 409
BsmAI GTCTC 2 cut(s) 140, 344
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 52
BsmI GAATGC 1 cut(s) 682
Bsp143I GATC 3 cut(s) 384, 655, 811
Bsp68I TCGCGA 1 cut(s) 810
BspACI CCGC 6 cut(s) 120, 160, 242, 363, 560, 627
BspCNI CTCAG 2 cut(s) 354, 673
BspFNI CGCG 1 cut(s) 810
BspLI GGNNCC 1 cut(s) 209
BspMI ACCTGC 1 cut(s) 359
BspPI GGATC 2 cut(s) 650, 806
BspQI GCTCTTC 1 cut(s) 502
BsrBI CCGCTC 1 cut(s) 363
BsrDI GCAATG 1 cut(s) 393
BssECI CCNNGG 3 cut(s) 377, 403, 794
BssMI GATC 3 cut(s) 384, 655, 811
BssT1I CCWWGG 1 cut(s) 794
Bst2UI CCWGG 2 cut(s) 405, 816
Bst4CI ACNGT 3 cut(s) 320, 605, 730
Bst6I CTCTTC 1 cut(s) 502
BstC8I GCNNGC 3 cut(s) 279, 400, 528
BstDEI CTNAG 4 cut(s) 110, 341, 388, 660
BstF5I GGATG 1 cut(s) 250
BstFNI CGCG 1 cut(s) 810
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 2 cut(s) 20, 116
BstKTI GATC 3 cut(s) 387, 658, 814
BstMAI GTCTC 2 cut(s) 140, 344
BstMBI GATC 3 cut(s) 384, 655, 811
BstMWI GCNNNNNNNGC 1 cut(s) 686
BstNI CCWGG 2 cut(s) 405, 816
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 3 cut(s) 376, 403, 814
BstUI CGCG 1 cut(s) 810
BstV1I GCAGC 1 cut(s) 628
BstV2I GAAGAC 2 cut(s) 365, 599
Bsu36I CCTNAGG 1 cut(s) 660
BsuI GTATCC 1 cut(s) 813
BtgZI GCGATG 1 cut(s) 457
BtsCI GGATG 1 cut(s) 250
BtsIMutI CAGTG 2 cut(s) 316, 735
BtuMI TCGCGA 1 cut(s) 810
BveI ACCTGC 1 cut(s) 359
Cac8I GCNNGC 3 cut(s) 279, 400, 528
CfoI GCGC 2 cut(s) 20, 116
CseI GACGC 1 cut(s) 85
Csp6I GTAC 2 cut(s) 88, 819
CviAII CATG 2 cut(s) 424, 461
CviJI RGCY 9 cut(s) 12, 132, 230, 345, 392, 530, 566, 596, 744
CviKI_1 RGCY 9 cut(s) 12, 132, 230, 345, 392, 530, 566, 596, 744
CviQI GTAC 2 cut(s) 88, 819
DdeI CTNAG 4 cut(s) 110, 341, 388, 660
DpnI GATC 3 cut(s) 386, 657, 813
DpnII GATC 3 cut(s) 384, 655, 811
DriI GACNNNNNGTC 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 502
Eam1105I GACNNNNNGTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 502
EciI GGCGGA 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 794
Eco81I CCTNAGG 1 cut(s) 660
EcoRII CCWGG 2 cut(s) 403, 814
EcoT14I CCWWGG 1 cut(s) 794
ErhI CCWWGG 1 cut(s) 794
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 2 cut(s) 427, 464
FaiI YATR 7 cut(s) 287, 307, 425, 434, 462, 500, 750
FaqI GGGAC 1 cut(s) 52
FatI CATG 2 cut(s) 423, 460
FauI CCCGC 1 cut(s) 167
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 1 cut(s) 642
FokI GGATG 1 cut(s) 257
Fsp4HI GCNGC 1 cut(s) 642
FspBI CTAG 1 cut(s) 783
FspI TGCGCA 1 cut(s) 19
GlaI GCGC 2 cut(s) 19, 115
GluI GCNGC 1 cut(s) 642
GsuI CTGGAG 1 cut(s) 807
HaeII RGCGCY 1 cut(s) 117
HapII CCGG 2 cut(s) 59, 377
HgaI GACGC 1 cut(s) 85
HhaI GCGC 2 cut(s) 20, 116
Hin1II CATG 2 cut(s) 427, 464
Hin6I GCGC 2 cut(s) 18, 114
HinP1I GCGC 2 cut(s) 18, 114
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 5 cut(s) 67, 155, 272, 428, 734
HpaII CCGG 2 cut(s) 59, 377
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 4 cut(s) 72, 299, 334, 696
Hpy188III TCNNGA 4 cut(s) 653, 662, 809, 824
Hpy8I GTNNAC 1 cut(s) 190
Hpy99I CGWCG 3 cut(s) 76, 79, 191
HpyAV CCTTC 1 cut(s) 65
HpyCH4III ACNGT 3 cut(s) 320, 605, 730
HpyCH4IV ACGT 2 cut(s) 149, 186
HpyCH4V TGCA 6 cut(s) 368, 578, 644, 680, 718, 726
HpyF10VI GCNNNNNNNGC 1 cut(s) 686
HpyF3I CTNAG 4 cut(s) 110, 341, 388, 660
HpySE526I ACGT 2 cut(s) 149, 186
Hsp92II CATG 2 cut(s) 427, 464
HspAI GCGC 2 cut(s) 18, 114
Kzo9I GATC 3 cut(s) 384, 655, 811
LguI GCTCTTC 1 cut(s) 502
LmnI GCTCC 2 cut(s) 360, 457
Lsp1109I GCAGC 1 cut(s) 628
LweI GCATC 2 cut(s) 71, 434
MaeI CTAG 1 cut(s) 783
MaeII ACGT 2 cut(s) 149, 186
MaeIII GTNAC 3 cut(s) 415, 470, 650
MalI GATC 3 cut(s) 386, 657, 813
MbiI CCGCTC 1 cut(s) 363
MboI GATC 3 cut(s) 384, 655, 811
MboII GAAGA 4 cut(s) 37, 365, 519, 599
MluCI AATT 5 cut(s) 123, 220, 517, 548, 690
MlyI GAGTC 2 cut(s) 266, 437
MmeI TCCRAC 3 cut(s) 95, 132, 812
MseI TTAA 1 cut(s) 234
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 2 cut(s) 59, 377
MspR9I CCNGG 3 cut(s) 378, 405, 816
Mva1269I GAATGC 1 cut(s) 682
MvaI CCWGG 2 cut(s) 405, 816
MvnI CGCG 1 cut(s) 810
MwoI GCNNNNNNNGC 1 cut(s) 686
NciI CCSGG 1 cut(s) 378
NdeII GATC 3 cut(s) 384, 655, 811
NlaIII CATG 2 cut(s) 427, 464
NlaIV GGNNCC 1 cut(s) 209
NmuCI GTSAC 3 cut(s) 415, 470, 650
NruI TCGCGA 1 cut(s) 810
NsbI TGCGCA 1 cut(s) 19
NspI RCATGY 1 cut(s) 464
PciSI GCTCTTC 1 cut(s) 502
PcsI WCGNNNNNNNCGW 3 cut(s) 71, 83, 743
PctI GAATGC 1 cut(s) 682
PfeI GAWTC 3 cut(s) 67, 155, 734
PkrI GCNGC 1 cut(s) 643
PleI GAGTC 2 cut(s) 266, 436
PpsI GAGTC 2 cut(s) 266, 436
PsiI TTATAA 1 cut(s) 500
Psp6I CCWGG 2 cut(s) 403, 814
PspGI CCWGG 2 cut(s) 403, 814
PspN4I GGNNCC 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 230
RruI TCGCGA 1 cut(s) 810
RsaI GTAC 2 cut(s) 89, 820
RsaNI GTAC 2 cut(s) 88, 819
SalI GTCGAC 1 cut(s) 188
SapI GCTCTTC 1 cut(s) 502
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 1 cut(s) 642
Sau3AI GATC 3 cut(s) 384, 655, 811
SchI GAGTC 2 cut(s) 266, 437
ScrFI CCNGG 3 cut(s) 378, 405, 816
SfaNI GCATC 2 cut(s) 71, 434
SpeI ACTAGT 1 cut(s) 782
Sse9I AATT 5 cut(s) 123, 220, 517, 548, 690
SsiI CCGC 6 cut(s) 120, 160, 242, 363, 560, 627
SspMI CTAG 1 cut(s) 783
StyD4I CCNGG 3 cut(s) 376, 403, 814
StyI CCWWGG 1 cut(s) 794
TaaI ACNGT 3 cut(s) 320, 605, 730
TaiI ACGT 2 cut(s) 152, 189
TaqI TCGA 4 cut(s) 65, 189, 351, 485
TaqII GACCGA 1 cut(s) 458
TasI AATT 5 cut(s) 123, 220, 517, 548, 690
TfiI GAWTC 3 cut(s) 67, 155, 734
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TscAI CASTG 2 cut(s) 323, 735
TseFI GTSAC 3 cut(s) 415, 470, 650
TseI GCWGC 1 cut(s) 641
Tsp45I GTSAC 3 cut(s) 415, 470, 650
TspDTI ATGAA 2 cut(s) 233, 449
TspGWI ACGGA 2 cut(s) 75, 700
TspRI CASTG 2 cut(s) 323, 735
XapI RAATTY 3 cut(s) 220, 517, 548
XceI RCATGY 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.