Rh7CG028000

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0008341
Physical Location & Seq
Forward (+)
2008429 .. 2011086
2658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG028000.1

Sequence Viewer

Length: 756 bp
ATGTTGTTGTTGGTTAGCCGTTGCGCACTAGTCCCATTCTCTCTGGGGACTTTCTTCAAACACCTTCCGCGCCCAAAACCCAGTCCCATCTTTCCTTACACAATCACACCCAAAACCCTAACCACCGCCACAATCCACTGCTCCTCTCACTTCGAAGCCTTGGACTCCCGCCAAAAACACCAAATCCATCTCTACGTCGACACCCTTCTCGAATGGAACCAGAGAATGAACTTGACCGCAGTCAGAGAAGCGGGTGAGGTAATGGAGAGGCACATTGAAGACTCGCTCGCGGTCATACCGCCCATAACAGACTCTTATCTCTCTCATTGTGGCGCCTCGTGTGGAAATGTCAGCGTTGTGGATGTGGGAAGCGGCGCGGGCCTGCCCGGGTTGGTTTTGGCCATTGCTTGCCCGGAATGGAAAGTGACCCTGATGGAGTCAATGAACAAAAGATGTGTTTTTTTGGAGCATGCTGTTAATCGTGTTGGTTTATCAAATGTGGAGATTGTTAGAGGAAGAGCAGAGAACTTGGGTCAGGATGTTTGCTTCAGGGAGAAATATGATGTAGCAGTGGCCAGAGCGGTTGCGGAGATGAGAATTTTGGGTAGGAATGTAAACAGTCTTTGTCTTGGTTTCGTGCGTGGATTTTCAATACTTAAAAGACTGGTTCCTTACATTGAGTTGTTGATTTTTTGTTACTTCGGTGGTCAGCGGAATACTGTCTTCCTCTGGTTCGCGTTGGTGGCTTGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GidB

Protein Analysis

251

Amino Acids

27.98

Weight (kDa)

8.54

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GidB PF02527 58 - 201 4.3e-41 rRNA small subunit methyltransferase G
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 25
AccB1I GGYRCC 1 cut(s) 332
AccBSI CCGCTC 1 cut(s) 581
AccI GTMKAC 1 cut(s) 198
AccII CGCG 4 cut(s) 70, 290, 377, 737
AcoI YGGCCR 2 cut(s) 399, 573
AcsI RAATTY 1 cut(s) 597
AcuI CTGAAG 1 cut(s) 532
AcyI GRCGYC 1 cut(s) 333
AgsI TTSAA 3 cut(s) 58, 278, 651
AhlI ACTAGT 1 cut(s) 28
Ama87I CYCGRG 1 cut(s) 386
AoxI GGCC 3 cut(s) 379, 399, 573
ApoI RAATTY 1 cut(s) 597
AspLEI GCGC 4 cut(s) 26, 72, 335, 377
AspS9I GGNCC 1 cut(s) 379
AsuC2I CCSGG 3 cut(s) 387, 388, 413
AsuHPI GGTGA 1 cut(s) 266
AsuII TTCGAA 1 cut(s) 153
AvaI CYCGRG 1 cut(s) 386
BalI TGGCCA 2 cut(s) 401, 575
BanI GGYRCC 1 cut(s) 332
BauI CACGAG 1 cut(s) 337
BbsI GAAGAC 2 cut(s) 285, 715
BccI CCATC 3 cut(s) 95, 195, 427
BceAI ACGGC 1 cut(s) 3
BcnI CCSGG 3 cut(s) 387, 388, 413
BcuI ACTAGT 1 cut(s) 28
BfaI CTAG 1 cut(s) 29
BfoI RGCGCY 1 cut(s) 336
BisI GCNGC 1 cut(s) 373
BlsI GCNGC 1 cut(s) 374
Bme1390I CCNGG 3 cut(s) 387, 388, 413
BmeT110I CYCGRG 1 cut(s) 386
BmgT120I GGNCC 1 cut(s) 379
BmiI GGNNCC 3 cut(s) 218, 334, 669
BmrFI CCNGG 3 cut(s) 387, 388, 413
BmrI ACTGGG 1 cut(s) 75
BmuI ACTGGG 1 cut(s) 75
BoxI GACNNNNGTC 1 cut(s) 239
BpiI GAAGAC 2 cut(s) 285, 715
Bpu14I TTCGAA 1 cut(s) 153
BpuMI CCSGG 3 cut(s) 387, 388, 413
BsaHI GRCGYC 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 159, 386
Bse1I ACTGG 2 cut(s) 81, 669
Bse3DI GCAATG 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 159, 386
BseGI GGATG 2 cut(s) 367, 544
BseMI GCAATG 1 cut(s) 402
BseNI ACTGG 2 cut(s) 81, 669
BseRI GAGGAG 1 cut(s) 133
Bsh1236I CGCG 4 cut(s) 70, 290, 377, 737
BshFI GGCC 3 cut(s) 381, 401, 575
BshNI GGYRCC 1 cut(s) 332
BsiHKCI CYCGRG 1 cut(s) 386
BsiSI CCGG 2 cut(s) 387, 413
BslFI GGGAC 3 cut(s) 17, 61, 69
BsmFI GGGAC 3 cut(s) 17, 61, 69
BsnI GGCC 3 cut(s) 381, 401, 575
BsoBI CYCGRG 1 cut(s) 386
Bsp119I TTCGAA 1 cut(s) 153
BspANI GGCC 3 cut(s) 381, 401, 575
BspFNI CGCG 4 cut(s) 70, 290, 377, 737
BspLI GGNNCC 3 cut(s) 218, 334, 669
BspQI GCTCTTC 1 cut(s) 511
BspT104I TTCGAA 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 332
BsrBI CCGCTC 1 cut(s) 581
BsrDI GCAATG 1 cut(s) 402
BsrI ACTGG 2 cut(s) 81, 669
BssECI CCNNGG 2 cut(s) 159, 386
BssNI GRCGYC 1 cut(s) 333
BssSI CACGAG 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 337
Bst4CI ACNGT 2 cut(s) 620, 721
Bst6I CTCTTC 1 cut(s) 511
BstACI GRCGYC 1 cut(s) 333
BstBI TTCGAA 1 cut(s) 153
BstC8I GCNNGC 5 cut(s) 288, 379, 383, 409, 471
BstF5I GGATG 2 cut(s) 367, 544
BstFNI CGCG 4 cut(s) 70, 290, 377, 737
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 4 cut(s) 26, 72, 335, 377
BstMWI GCNNNNNNNGC 2 cut(s) 378, 743
BstNSI RCATGY 1 cut(s) 473
BstPAI GACNNNNGTC 1 cut(s) 239
BstSCI CCNGG 3 cut(s) 385, 386, 411
BstUI CGCG 4 cut(s) 70, 290, 377, 737
BstV2I GAAGAC 2 cut(s) 285, 715
BsuRI GGCC 3 cut(s) 381, 401, 575
BtsCI GGATG 2 cut(s) 367, 544
BtsI GCAGTG 2 cut(s) 136, 576
BtsIMutI CAGTG 2 cut(s) 136, 576
Cac8I GCNNGC 5 cut(s) 288, 379, 383, 409, 471
CfoI GCGC 4 cut(s) 26, 72, 335, 377
Cfr13I GGNCC 1 cut(s) 379
Cfr9I CCCGGG 1 cut(s) 386
CviAII CATG 1 cut(s) 470
CviJI RGCY 6 cut(s) 18, 158, 381, 401, 575, 746
CviKI_1 RGCY 6 cut(s) 18, 158, 381, 401, 575, 746
DinI GGCGCC 1 cut(s) 334
EaeI YGGCCR 2 cut(s) 399, 573
Eam1104I CTCTTC 1 cut(s) 511
EarI CTCTTC 1 cut(s) 511
Eco130I CCWWGG 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 532
Eco88I CYCGRG 1 cut(s) 386
EcoT14I CCWWGG 1 cut(s) 159
EgeI GGCGCC 1 cut(s) 334
EheI GGCGCC 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 1 cut(s) 473
FaiI YATR 4 cut(s) 296, 305, 471, 561
FaqI GGGAC 3 cut(s) 17, 61, 69
FatI CATG 1 cut(s) 469
FauI CCCGC 3 cut(s) 176, 244, 370
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 1 cut(s) 373
FokI GGATG 2 cut(s) 374, 551
Fsp4HI GCNGC 1 cut(s) 373
FspBI CTAG 1 cut(s) 29
FspI TGCGCA 1 cut(s) 25
GlaI GCGC 4 cut(s) 25, 71, 334, 376
GluI GCNGC 1 cut(s) 373
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 3 cut(s) 381, 401, 575
HapII CCGG 2 cut(s) 387, 413
HhaI GCGC 4 cut(s) 26, 72, 335, 377
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 1 cut(s) 473
Hin6I GCGC 4 cut(s) 24, 70, 333, 375
HinP1I GCGC 4 cut(s) 24, 70, 333, 375
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 4 cut(s) 164, 281, 311, 437
HpaII CCGG 2 cut(s) 387, 413
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 2 cut(s) 199, 616
Hpy188I TCNGA 1 cut(s) 245
Hpy188III TCNNGA 2 cut(s) 209, 536
Hpy8I GTNNAC 2 cut(s) 199, 616
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 2 cut(s) 74, 215
HpyCH4III ACNGT 2 cut(s) 620, 721
HpyCH4IV ACGT 1 cut(s) 195
HpyF10VI GCNNNNNNNGC 2 cut(s) 378, 743
HpySE526I ACGT 1 cut(s) 195
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 1 cut(s) 473
HspAI GCGC 4 cut(s) 24, 70, 333, 375
KasI GGCGCC 1 cut(s) 332
LguI GCTCTTC 1 cut(s) 511
LmnI GCTCC 2 cut(s) 146, 466
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 2 cut(s) 424, 695
MbiI CCGCTC 1 cut(s) 581
MboII GAAGA 4 cut(s) 46, 290, 528, 715
MlsI TGGCCA 2 cut(s) 401, 575
MluCI AATT 1 cut(s) 597
MluNI TGGCCA 2 cut(s) 401, 575
Mly113I GGCGCC 1 cut(s) 333
MlyI GAGTC 4 cut(s) 158, 275, 305, 446
MnlI CCTC 6 cut(s) 154, 250, 261, 346, 506, 737
Mox20I TGGCCA 2 cut(s) 401, 575
MscI TGGCCA 2 cut(s) 401, 575
MseI TTAA 2 cut(s) 477, 657
Msp20I TGGCCA 2 cut(s) 401, 575
MspA1I CMGCKG 1 cut(s) 712
MspI CCGG 2 cut(s) 387, 413
MspR9I CCNGG 3 cut(s) 387, 388, 413
MvnI CGCG 4 cut(s) 70, 290, 377, 737
MwoI GCNNNNNNNGC 2 cut(s) 378, 743
NarI GGCGCC 1 cut(s) 333
NciI CCSGG 3 cut(s) 387, 388, 413
NlaIII CATG 1 cut(s) 473
NlaIV GGNNCC 3 cut(s) 218, 334, 669
NmuCI GTSAC 1 cut(s) 424
NsbI TGCGCA 1 cut(s) 25
NspI RCATGY 1 cut(s) 473
NspV TTCGAA 1 cut(s) 153
PaeI GCATGC 1 cut(s) 473
PciSI GCTCTTC 1 cut(s) 511
PkrI GCNGC 1 cut(s) 374
PleI GAGTC 4 cut(s) 158, 275, 305, 445
PluTI GGCGCC 1 cut(s) 336
PpsI GAGTC 4 cut(s) 158, 275, 305, 445
PshAI GACNNNNGTC 1 cut(s) 239
PspN4I GGNNCC 3 cut(s) 218, 334, 669
PspPI GGNCC 1 cut(s) 379
SalI GTCGAC 1 cut(s) 197
SapI GCTCTTC 1 cut(s) 511
SaqAI TTAA 2 cut(s) 477, 657
SatI GCNGC 1 cut(s) 373
Sau96I GGNCC 1 cut(s) 379
SchI GAGTC 4 cut(s) 158, 275, 305, 446
ScrFI CCNGG 3 cut(s) 387, 388, 413
SetI ASST 3 cut(s) 66, 198, 261
SfoI GGCGCC 1 cut(s) 334
SfuI TTCGAA 1 cut(s) 153
SmaI CCCGGG 1 cut(s) 388
SpeI ACTAGT 1 cut(s) 28
SphI GCATGC 1 cut(s) 473
Sse9I AATT 1 cut(s) 597
SspDI GGCGCC 1 cut(s) 332
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 385, 386, 411
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 620, 721
TaiI ACGT 1 cut(s) 198
TaqI TCGA 3 cut(s) 153, 198, 210
TasI AATT 1 cut(s) 597
TauI GCSGC 1 cut(s) 375
Tru1I TTAA 2 cut(s) 477, 657
Tru9I TTAA 2 cut(s) 477, 657
TscAI CASTG 2 cut(s) 143, 576
TseFI GTSAC 1 cut(s) 424
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 242, 458
TspMI CCCGGG 1 cut(s) 386
TspRI CASTG 2 cut(s) 143, 576
XapI RAATTY 1 cut(s) 597
XceI RCATGY 1 cut(s) 473
XmaI CCCGGG 1 cut(s) 386
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.