AT5G50700

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010709
Physical Location & Seq
Reverse (-)
20623024 .. 20625279
2256 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50700.1

Sequence Viewer

Length: 1050 bp
ATGGAGTTGATAAACGACTTTCTCAACCTAACTGCTCCTTTCTTCACCTTCTTTGGTCTCTGCTTCTTCTTGCCTCCTTTTTACTTCTTCAAGTTCTTGCAGTCTATTTTCTCGACAATTTTCTCTGAAAATCTTTACGGGAAAGTTGTTCTCATCACTGGTGCTTCCTCCGGTATAGGCGAGCAATTGGCATATGAGTACGCATGTAGAGGTGCATGTTTAGCCCTGACCGCCCGAAGGAAGAACCGTCTAGAGGAAGTGGCAGAGATTGCTCGTGAACTCGGATCTCCCAATGTTGTTACCGTTCATGCTGATGTCTCCAAACCTGATGACTGTAGACGAATCGTTGATGACACCATCACCCATTTTGGCAGATTGGATCATCTTGTAAATAATGCTGGGATGACGCAGATTTCAATGTTTGAGAACATTGAAGATATAACGAGGACAAAAGCAGTTTTGGATACTAACTTCTGGGGATCGGTTTATACCACTCGTGCTGCGCTTCCATACCTTAGACAAAGTAATGGTAAGATTGTGGCTATGTCGTCTTCCGCAGCGTGGCTAACCGCTCCAAGGATGAGTTTTTACAATGCAAGCAAAGCAGCTTTGTTGAGCTTCTTTGAGACGATGAGAATTGAGCTTGGTGGCGATGTACACATTACAATCGTCACACCTGGTTATATCGAGTCGGAGCTCACACAAGGCAAGTACTTCTCTGGTGAAGGCGAGTTAATAGTCAACCAAGACATGAGAGATGTTCAAGTAGGACCATTTCCGGTAGCGTCAGCATCAGGATGTGCCAAGTCGATAGTGAACGGTGTGTGCCGGAAACAAAGATACGTGACAGAGCCATCATGGTTTAAGGTGACGTACCTTTGGAAAGTGCTATGTCCGGAGTTGATTGAGTGGGGTTGTCGGTTACTGTACATGACTGGAACTGGTATGTCCGAGGATACTGCACTCAACAAGAGGATCATGGACATTCCTGGTGTACGTAGTACTCTGTACCCGGAATCTATCAGAACTCCAGAAATCAAGTCGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005811 GO:0016020 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

349

Amino Acids

39.09

Weight (kDa)

5.92

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 48 - 235 1.4e-47 short chain dehydrogenase
KR PF08659 50 - 212 2.4e-12 KR domain
adh_short_C2 PF13561 54 - 236 1.9e-34 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010709)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50600 AT5G50700
fragaria_vesca FvH4_5g08690 FvH4_5g08690 FvH4_5g08690
malus_domestica MD14G1153000.v1.1
prunus_persica Prupe.5G148100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0189581 RchiOBHm_Chr7g0189691
rosa_laevigata RLG00000004597
rosa_multiflora Rmu_sc0001924.1_g000017
rosa_roxburghii Rroxscaffold_3G00265260
rosa_rugosa Rorug06G0499100
rosa_samantha Rh7AG104200 Rh7BG106800 Rh7CG108900 Rh7DG106200 Rh7DG107300
rosa_wichuraiana Rw7G008990 Rw7G009020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 572
AccI GTMKAC 1 cut(s) 337
AccIII TCCGGA 1 cut(s) 895
AciI CCGC 3 cut(s) 231, 555, 570
AclWI GGATC 4 cut(s) 292, 387, 487, 983
AfaI GTAC 8 cut(s) 200, 657, 713, 875, 929, 996, 1003, 1010
AfiI CCNNNNNNNGG 4 cut(s) 237, 253, 561, 576
AgsI TTSAA 4 cut(s) 91, 417, 434, 764
AjnI CCWGG 2 cut(s) 676, 988
AluBI AGCT 4 cut(s) 608, 618, 643, 697
AluI AGCT 4 cut(s) 608, 618, 643, 697
Alw21I GWGCWC 1 cut(s) 699
Alw26I GTCTC 3 cut(s) 62, 322, 620
AlwI GGATC 4 cut(s) 292, 387, 487, 983
Aor13HI TCCGGA 1 cut(s) 895
ApeKI GCWGC 3 cut(s) 500, 557, 605
AspLEI GCGC 1 cut(s) 505
AspS9I GGNCC 1 cut(s) 770
AsuC2I CCSGG 1 cut(s) 1013
AsuHPI GGTGA 4 cut(s) 37, 352, 734, 880
AvaII GGWCC 1 cut(s) 770
BanII GRGCYC 1 cut(s) 699
BauI CACGAG 2 cut(s) 273, 495
BbsI GAAGAC 1 cut(s) 543
Bbv12I GWGCWC 1 cut(s) 699
BbvI GCAGC 3 cut(s) 487, 569, 617
BccI CCATC 2 cut(s) 365, 862
BcgI CGANNNNNNTGC 2 cut(s) 941, 975
BciT130I CCWGG 2 cut(s) 678, 990
BciVI GTATCC 2 cut(s) 457, 949
BcnI CCSGG 1 cut(s) 1013
BcoDI GTCTC 3 cut(s) 62, 322, 620
BfaI CTAG 1 cut(s) 251
BfmI CTRYAG 1 cut(s) 334
BfuI GTATCC 2 cut(s) 457, 949
BisI GCNGC 3 cut(s) 501, 558, 606
BlsI GCNGC 3 cut(s) 502, 559, 607
BmcAI AGTACT 2 cut(s) 713, 1003
Bme1390I CCNGG 3 cut(s) 678, 990, 1013
Bme18I GGWCC 1 cut(s) 770
BmgT120I GGNCC 1 cut(s) 770
BmrFI CCNGG 3 cut(s) 678, 990, 1013
BmsI GCATC 1 cut(s) 800
BpiI GAAGAC 1 cut(s) 543
BpmI CTGGAG 1 cut(s) 1014
BpuMI CCSGG 1 cut(s) 1013
BsaAI YACGTR 2 cut(s) 844, 998
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 2 cut(s) 575, 951
BsaWI WCCGGW 3 cut(s) 170, 778, 895
Bsc4I CCNNNNNNNGG 4 cut(s) 237, 253, 561, 576
Bse1I ACTGG 3 cut(s) 163, 940, 946
BseAI TCCGGA 1 cut(s) 895
BseBI CCWGG 2 cut(s) 678, 990
BseDI CCNNGG 2 cut(s) 575, 951
BseGI GGATG 3 cut(s) 408, 585, 803
BseLI CCNNNNNNNGG 4 cut(s) 237, 253, 561, 576
BseNI ACTGG 3 cut(s) 163, 940, 946
BseXI GCAGC 3 cut(s) 487, 569, 617
BseYI CCCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 945
BsiHKAI GWGCWC 1 cut(s) 699
BsiSI CCGG 5 cut(s) 171, 779, 829, 896, 1013
BslI CCNNNNNNNGG 4 cut(s) 237, 253, 561, 576
BsmAI GTCTC 3 cut(s) 62, 322, 620
BsmBI CGTCTC 1 cut(s) 620
Bso31I GGTCTC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 699
Bsp13I TCCGGA 1 cut(s) 895
Bsp1407I TGTACA 2 cut(s) 655, 927
Bsp143I GATC 4 cut(s) 284, 379, 479, 975
BspACI CCGC 3 cut(s) 231, 555, 570
BspEI TCCGGA 1 cut(s) 895
BspPI GGATC 4 cut(s) 292, 387, 487, 983
BspTNI GGTCTC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 572
BsrGI TGTACA 2 cut(s) 655, 927
BsrI ACTGG 3 cut(s) 163, 940, 946
BssECI CCNNGG 2 cut(s) 575, 951
BssMI GATC 4 cut(s) 284, 379, 479, 975
BssSI CACGAG 2 cut(s) 273, 495
BssT1I CCWWGG 1 cut(s) 575
Bst2BI CACGAG 2 cut(s) 273, 495
Bst2UI CCWGG 2 cut(s) 678, 990
Bst4CI ACNGT 5 cut(s) 248, 304, 335, 821, 927
BstAPI GCANNNNNTGC 1 cut(s) 269
BstAUI TGTACA 2 cut(s) 655, 927
BstBAI YACGTR 2 cut(s) 844, 998
BstC8I GCNNGC 2 cut(s) 182, 598
BstDEI CTNAG 1 cut(s) 515
BstF5I GGATG 3 cut(s) 408, 585, 803
BstHHI GCGC 1 cut(s) 505
BstKTI GATC 4 cut(s) 287, 382, 482, 978
BstMAI GTCTC 3 cut(s) 62, 322, 620
BstMBI GATC 4 cut(s) 284, 379, 479, 975
BstMWI GCNNNNNNNGC 4 cut(s) 221, 230, 269, 602
BstNI CCWGG 2 cut(s) 678, 990
BstNSI RCATGY 2 cut(s) 207, 219
BstSCI CCNGG 3 cut(s) 676, 988, 1011
BstSFI CTRYAG 1 cut(s) 334
BstSNI TACGTA 1 cut(s) 998
BstV1I GCAGC 3 cut(s) 487, 569, 617
BstV2I GAAGAC 1 cut(s) 543
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
BsuI GTATCC 2 cut(s) 457, 949
BtgZI GCGATG 1 cut(s) 666
BtsCI GGATG 3 cut(s) 408, 585, 803
BtsIMutI CAGTG 1 cut(s) 156
Cac8I GCNNGC 2 cut(s) 182, 598
CfoI GCGC 1 cut(s) 505
Cfr13I GGNCC 1 cut(s) 770
CseI GACGC 2 cut(s) 415, 774
CsiI ACCWGGT 1 cut(s) 676
Csp6I GTAC 8 cut(s) 199, 656, 712, 874, 928, 995, 1002, 1009
CviAII CATG 7 cut(s) 204, 216, 308, 751, 858, 931, 979
CviJI RGCY 8 cut(s) 224, 542, 565, 608, 618, 643, 697, 853
CviKI_1 RGCY 8 cut(s) 224, 542, 565, 608, 618, 643, 697, 853
CviQI GTAC 8 cut(s) 199, 656, 712, 874, 928, 995, 1002, 1009
DdeI CTNAG 1 cut(s) 515
DpnI GATC 4 cut(s) 286, 381, 481, 977
DpnII GATC 4 cut(s) 284, 379, 479, 975
Ecl136II GAGCTC 1 cut(s) 697
Eco105I TACGTA 1 cut(s) 998
Eco130I CCWWGG 1 cut(s) 575
Eco24I GRGCYC 1 cut(s) 699
Eco31I GGTCTC 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 770
Eco53kI GAGCTC 1 cut(s) 697
EcoICRI GAGCTC 1 cut(s) 697
EcoRII CCWGG 2 cut(s) 676, 988
EcoT14I CCWWGG 1 cut(s) 575
EcoT38I GRGCYC 1 cut(s) 699
ErhI CCWWGG 1 cut(s) 575
Esp3I CGTCTC 1 cut(s) 620
FaeI CATG 7 cut(s) 207, 219, 311, 754, 861, 934, 982
FatI CATG 7 cut(s) 203, 215, 307, 750, 857, 930, 978
FauNDI CATATG 1 cut(s) 193
FblI GTMKAC 1 cut(s) 337
Fnu4HI GCNGC 3 cut(s) 501, 558, 606
FokI GGATG 3 cut(s) 415, 592, 810
FriOI GRGCYC 1 cut(s) 699
Fsp4HI GCNGC 3 cut(s) 501, 558, 606
FspBI CTAG 1 cut(s) 251
GlaI GCGC 1 cut(s) 504
GluI GCNGC 3 cut(s) 501, 558, 606
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 1 cut(s) 1014
HapII CCGG 5 cut(s) 171, 779, 829, 896, 1013
HgaI GACGC 2 cut(s) 415, 774
HhaI GCGC 1 cut(s) 505
Hin1II CATG 7 cut(s) 207, 219, 311, 754, 861, 934, 982
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HinfI GANTC 3 cut(s) 342, 689, 1016
HpaII CCGG 5 cut(s) 171, 779, 829, 896, 1013
HphI GGTGA 4 cut(s) 37, 352, 734, 880
Hpy166II GTNNAC 6 cut(s) 278, 338, 658, 742, 817, 995
Hpy188I TCNGA 6 cut(s) 127, 284, 694, 952, 1025, 1045
Hpy188III TCNNGA 6 cut(s) 112, 251, 275, 795, 896, 1031
Hpy8I GTNNAC 6 cut(s) 278, 338, 658, 742, 817, 995
HpyAV CCTTC 3 cut(s) 58, 231, 719
HpyCH4III ACNGT 5 cut(s) 248, 304, 335, 821, 927
HpyCH4IV ACGT 3 cut(s) 843, 872, 997
HpyCH4V TGCA 4 cut(s) 100, 215, 596, 962
HpyF10VI GCNNNNNNNGC 4 cut(s) 221, 230, 269, 602
HpyF3I CTNAG 1 cut(s) 515
HpySE526I ACGT 3 cut(s) 843, 872, 997
Hsp92II CATG 7 cut(s) 207, 219, 311, 754, 861, 934, 982
HspAI GCGC 1 cut(s) 503
Kpn2I TCCGGA 1 cut(s) 895
Kzo9I GATC 4 cut(s) 284, 379, 479, 975
LmnI GCTCC 3 cut(s) 40, 577, 694
Lsp1109I GCAGC 3 cut(s) 487, 569, 617
LweI GCATC 1 cut(s) 800
MabI ACCWGGT 1 cut(s) 676
MaeI CTAG 1 cut(s) 251
MaeII ACGT 3 cut(s) 843, 872, 997
MaeIII GTNAC 5 cut(s) 298, 670, 844, 868, 921
MalI GATC 4 cut(s) 286, 381, 481, 977
MbiI CCGCTC 1 cut(s) 572
MboI GATC 4 cut(s) 284, 379, 479, 975
MboII GAAGA 6 cut(s) 34, 58, 79, 253, 446, 543
MfeI CAATTG 1 cut(s) 185
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 1 cut(s) 699
MluCI AATT 3 cut(s) 117, 185, 636
MlyI GAGTC 1 cut(s) 698
MmeI TCCRAC 2 cut(s) 672, 1023
MnlI CCTC 7 cut(s) 84, 178, 203, 247, 438, 946, 966
MroI TCCGGA 1 cut(s) 895
MseI TTAA 2 cut(s) 734, 864
MslI CAYNNNNRTG 2 cut(s) 312, 796
MspI CCGG 5 cut(s) 171, 779, 829, 896, 1013
MspR9I CCNGG 3 cut(s) 678, 990, 1013
MunI CAATTG 1 cut(s) 185
MvaI CCWGG 2 cut(s) 678, 990
MwoI GCNNNNNNNGC 4 cut(s) 221, 230, 269, 602
NciI CCSGG 1 cut(s) 1013
NdeI CATATG 1 cut(s) 193
NdeII GATC 4 cut(s) 284, 379, 479, 975
NlaIII CATG 7 cut(s) 207, 219, 311, 754, 861, 934, 982
NmuCI GTSAC 3 cut(s) 670, 844, 868
NspI RCATGY 2 cut(s) 207, 219
PfeI GAWTC 2 cut(s) 342, 1016
PkrI GCNGC 3 cut(s) 502, 559, 607
PleI GAGTC 1 cut(s) 697
PpsI GAGTC 1 cut(s) 697
Ppu21I YACGTR 2 cut(s) 844, 998
Psp124BI GAGCTC 1 cut(s) 699
Psp6I CCWGG 2 cut(s) 676, 988
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 2 cut(s) 676, 988
PspPI GGNCC 1 cut(s) 770
PsuI RGATCY 1 cut(s) 284
RsaI GTAC 8 cut(s) 200, 657, 713, 875, 929, 996, 1003, 1010
RsaNI GTAC 8 cut(s) 199, 656, 712, 874, 928, 995, 1002, 1009
RseI CAYNNNNRTG 2 cut(s) 312, 796
SacI GAGCTC 1 cut(s) 699
SaqAI TTAA 2 cut(s) 734, 864
SatI GCNGC 3 cut(s) 501, 558, 606
Sau3AI GATC 4 cut(s) 284, 379, 479, 975
Sau96I GGNCC 1 cut(s) 770
ScaI AGTACT 2 cut(s) 713, 1003
SchI GAGTC 1 cut(s) 698
ScrFI CCNGG 3 cut(s) 678, 990, 1013
SduI GDGCHC 1 cut(s) 699
SexAI ACCWGGT 1 cut(s) 676
SfaNI GCATC 1 cut(s) 800
SfcI CTRYAG 1 cut(s) 334
SinI GGWCC 1 cut(s) 770
SmiMI CAYNNNNRTG 2 cut(s) 312, 796
SnaBI TACGTA 1 cut(s) 998
Sse9I AATT 3 cut(s) 117, 185, 636
SsiI CCGC 3 cut(s) 231, 555, 570
SspMI CTAG 1 cut(s) 251
SstI GAGCTC 1 cut(s) 699
StyD4I CCNGG 3 cut(s) 676, 988, 1011
StyI CCWWGG 1 cut(s) 575
TaaI ACNGT 5 cut(s) 248, 304, 335, 821, 927
TaiI ACGT 3 cut(s) 846, 875, 1000
TaqI TCGA 3 cut(s) 113, 687, 809
TasI AATT 3 cut(s) 117, 185, 636
TatI WGTACW 4 cut(s) 655, 711, 927, 1001
TfiI GAWTC 2 cut(s) 342, 1016
Tru1I TTAA 2 cut(s) 734, 864
Tru9I TTAA 2 cut(s) 734, 864
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 3 cut(s) 670, 844, 868
TseI GCWGC 3 cut(s) 500, 557, 605
Tsp45I GTSAC 3 cut(s) 670, 844, 868
TspDTI ATGAA 1 cut(s) 296
TspRI CASTG 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 770
XbaI TCTAGA 1 cut(s) 250
XceI RCATGY 2 cut(s) 207, 219
XmiI GTMKAC 1 cut(s) 337
XspI CTAG 1 cut(s) 251
ZrmI AGTACT 2 cut(s) 713, 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.