MD14G1153000.v1.1

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010709
Physical Location & Seq
Reverse (-)
24714054 .. 24716211
2158 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1153000.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGTTGGGTCTTATTCACACGTTCTTGAATCTTGTAGCTCCTCCTTTCACCTTCTTCTCCCTCTGCGTTTTCCTGCCACCCTTTTATCTCTTCAAGTCCTTCTCCTATGTCCTGAGCTCCATTTTCAGTGAAAATCTCAAAGGAAAGGTCGTCGTCATAACCGGGGCCTCCTCTGGTATTGGAGAGCACTTGGCTTATGAGTATGCGAAGAGAGGGGCACGGTTAGCCCTTGTTGGGAGGAGTGAAAACGCACTCAGAGAAGTTGTTGATCGAGCAAGTGATTTTGGCTCTCCAGATGTTCTCATGATACGTGCAGATGTTTCCAAGGTTGAGGACTGCAAGAGAATAGTTGATGACACCATGAACCACTATGGAAGGCTAGACCATCTGGTGAACAATGCTGGGATCACTTTACTTAGCATATTGGAAGACGCAACTGATATCACCAACTTCAGAACCATCATGGACACAAACTTCTGGGGCTCGGTTTATACCACTCATTTTGCACTTCCACACCTGAAAAGCAGCAAAGGTAAAATCGTGGTACTTTCGTCGTCTGCTTCATGGCTACCTATACCAAGACAAAGCATTTATAATGCAAGTAAAGCAGCTCTACTAAGCATGTACGAGACACTGAGGATTGAATTTGGGCCAGACATCCAAATTACAATCGTGACTCCTGGATTCATAGAGTCTGAGCTGACCCAAGGCAAGTTCTTAATGAGTGAAGGCAAAATGACTGTTGATCAAGATTTGAGAGATGCCCAAGTAAGTGCAACTCCAGTTGCTAAAGTGGAAGGATGTGCCAAATCAATCGTGAACAGCGCTTGTCGTGGGGATAGGTACTTGACGGAGCCGGCATGGTTCAGGGTGACATACTTATGGAAGGTGTTTTGCCCTGAGCTACTTGAGTGGGGTTACAGACTGATTTATATGAATAGGGCGGGCTCATCTGCCAGAGATGCACCTAGCGAGAAGATCTTGGATTACACAGGAGCCAAGAATGTGCTTTACCCTGAGAGCCTCCATACTCCTGAAGTCAAAACAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005811 GO:0016020 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

351

Amino Acids

39.03

Weight (kDa)

6.4

Isoelectric Point (pI)

32.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 49 - 237 1.8e-46 short chain dehydrogenase
KR PF08659 51 - 210 4.5e-12 KR domain
adh_short_C2 PF13561 55 - 237 9.4e-35 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010709)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50600 AT5G50700
fragaria_vesca FvH4_5g08690 FvH4_5g08690 FvH4_5g08690
malus_domestica MD14G1153000.v1.1
prunus_persica Prupe.5G148100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0189581 RchiOBHm_Chr7g0189691
rosa_laevigata RLG00000004597
rosa_multiflora Rmu_sc0001924.1_g000017
rosa_roxburghii Rroxscaffold_3G00265260
rosa_rugosa Rorug06G0499100
rosa_samantha Rh7AG104200 Rh7BG106800 Rh7CG108900 Rh7DG106200 Rh7DG107300
rosa_wichuraiana Rw7G008990 Rw7G009020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 594
AciI CCGC 1 cut(s) 944
AclWI GGATC 1 cut(s) 413
AcsI RAATTY 1 cut(s) 644
AcuI CTGAAG 1 cut(s) 436
AfaI GTAC 3 cut(s) 546, 626, 845
AfeI AGCGCT 1 cut(s) 826
AfiI CCNNNNNNNGG 1 cut(s) 234
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 3 cut(s) 28, 94, 644
AjnI CCWGG 1 cut(s) 679
AluBI AGCT 5 cut(s) 38, 117, 611, 700, 904
AluI AGCT 5 cut(s) 38, 117, 611, 700, 904
Alw21I GWGCWC 2 cut(s) 119, 189
Alw26I GTCTC 1 cut(s) 623
AlwI GGATC 1 cut(s) 413
Aor51HI AGCGCT 1 cut(s) 826
AoxI GGCC 2 cut(s) 165, 650
ApeKI GCWGC 2 cut(s) 525, 608
ApoI RAATTY 1 cut(s) 644
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
AspLEI GCGC 1 cut(s) 827
AspS9I GGNCC 2 cut(s) 165, 650
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 4 cut(s) 40, 403, 436, 883
BaeGI GKGCMC 1 cut(s) 220
BaeI ACNNNNGTAYC 2 cut(s) 536, 569
BanII GRGCYC 3 cut(s) 119, 485, 950
BbsI GAAGAC 1 cut(s) 435
Bbv12I GWGCWC 2 cut(s) 119, 189
BbvI GCAGC 2 cut(s) 537, 620
BccI CCATC 2 cut(s) 393, 467
BciT130I CCWGG 1 cut(s) 681
BclI TGATCA 1 cut(s) 745
BcnI CCSGG 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 623
BfaI CTAG 2 cut(s) 380, 969
BfoI RGCGCY 1 cut(s) 828
BglII AGATCT 1 cut(s) 978
BisI GCNGC 2 cut(s) 526, 609
BlsI GCNGC 2 cut(s) 527, 610
Bme1390I CCNGG 2 cut(s) 163, 681
BmgT120I GGNCC 2 cut(s) 165, 650
BmiI GGNNCC 3 cut(s) 166, 855, 997
BmrFI CCNGG 2 cut(s) 163, 681
BmsI GCATC 2 cut(s) 751, 952
BpiI GAAGAC 1 cut(s) 435
BpmI CTGGAG 2 cut(s) 276, 765
Bpu10I CCTNAGC 2 cut(s) 113, 900
BpuEI CTTGAG 1 cut(s) 929
BpuMI CCSGG 1 cut(s) 163
BsaAI YACGTR 1 cut(s) 311
BsaJI CCNNGG 3 cut(s) 162, 324, 706
Bsc4I CCNNNNNNNGG 1 cut(s) 234
Bse118I RCCGGY 1 cut(s) 856
Bse1I ACTGG 1 cut(s) 782
BseBI CCWGG 1 cut(s) 681
BseDI CCNNGG 3 cut(s) 162, 324, 706
BseGI GGATG 2 cut(s) 657, 806
BseLI CCNNNNNNNGG 1 cut(s) 234
BseMII CTCAG 6 cut(s) 104, 268, 626, 687, 891, 1008
BseNI ACTGG 1 cut(s) 782
BseRI GAGGAG 3 cut(s) 30, 160, 253
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 2 cut(s) 537, 620
BseYI CCCAGC 1 cut(s) 401
BsgI GTGCAG 1 cut(s) 333
BshFI GGCC 2 cut(s) 167, 652
BsiHKAI GWGCWC 2 cut(s) 119, 189
BsiSI CCGG 2 cut(s) 162, 857
BslI CCNNNNNNNGG 1 cut(s) 234
BsmAI GTCTC 1 cut(s) 623
BsnI GGCC 2 cut(s) 167, 652
Bsp1286I GDGCHC 5 cut(s) 119, 189, 220, 485, 950
Bsp143I GATC 4 cut(s) 268, 405, 745, 978
BspACI CCGC 1 cut(s) 944
BspANI GGCC 2 cut(s) 167, 652
BspCNI CTCAG 6 cut(s) 105, 267, 627, 688, 892, 1009
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 3 cut(s) 166, 855, 997
BspPI GGATC 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 856
BsrI ACTGG 1 cut(s) 782
BssAI RCCGGY 1 cut(s) 856
BssECI CCNNGG 3 cut(s) 162, 324, 706
BssMI GATC 4 cut(s) 268, 405, 745, 978
BssT1I CCWWGG 2 cut(s) 324, 706
Bst2UI CCWGG 1 cut(s) 681
Bst4CI ACNGT 2 cut(s) 222, 742
Bst6I CTCTTC 2 cut(s) 95, 203
BstBAI YACGTR 1 cut(s) 311
BstC8I GCNNGC 2 cut(s) 858, 946
BstDEI CTNAG 8 cut(s) 113, 254, 416, 617, 635, 696, 900, 1017
BstF5I GGATG 2 cut(s) 657, 806
BstH2I RGCGCY 1 cut(s) 828
BstHHI GCGC 1 cut(s) 827
BstKTI GATC 4 cut(s) 271, 408, 748, 981
BstMAI GTCTC 1 cut(s) 623
BstMBI GATC 4 cut(s) 268, 405, 745, 978
BstMWI GCNNNNNNNGC 3 cut(s) 224, 605, 962
BstNI CCWGG 1 cut(s) 681
BstNSI RCATGY 1 cut(s) 625
BstSCI CCNGG 2 cut(s) 161, 679
BstSLI GKGCMC 1 cut(s) 220
BstV1I GCAGC 2 cut(s) 537, 620
BstV2I GAAGAC 1 cut(s) 435
BstX2I RGATCY 1 cut(s) 978
BstYI RGATCY 1 cut(s) 978
BsuRI GGCC 2 cut(s) 167, 652
BtsCI GGATG 2 cut(s) 657, 806
BtsIMutI CAGTG 2 cut(s) 133, 632
Cac8I GCNNGC 2 cut(s) 858, 946
CciI TCATGA 1 cut(s) 303
CfoI GCGC 1 cut(s) 827
Cfr10I RCCGGY 1 cut(s) 856
Cfr13I GGNCC 2 cut(s) 165, 650
CseI GACGC 1 cut(s) 440
Csp6I GTAC 3 cut(s) 545, 625, 844
CspCI CAANNNNNGTGG 2 cut(s) 484, 519
CviAII CATG 6 cut(s) 304, 361, 463, 564, 622, 861
CviQI GTAC 3 cut(s) 545, 625, 844
DdeI CTNAG 8 cut(s) 113, 254, 416, 617, 635, 696, 900, 1017
DpnI GATC 4 cut(s) 270, 407, 747, 980
DpnII GATC 4 cut(s) 268, 405, 745, 978
Eam1104I CTCTTC 2 cut(s) 95, 203
EarI CTCTTC 2 cut(s) 95, 203
Ecl136II GAGCTC 1 cut(s) 117
Eco130I CCWWGG 2 cut(s) 324, 706
Eco24I GRGCYC 3 cut(s) 119, 485, 950
Eco32I GATATC 1 cut(s) 442
Eco47III AGCGCT 1 cut(s) 826
Eco53kI GAGCTC 1 cut(s) 117
Eco57I CTGAAG 1 cut(s) 436
EcoICRI GAGCTC 1 cut(s) 117
EcoO109I RGGNCCY 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 679
EcoRV GATATC 1 cut(s) 442
EcoT14I CCWWGG 2 cut(s) 324, 706
EcoT38I GRGCYC 3 cut(s) 119, 485, 950
ErhI CCWWGG 2 cut(s) 324, 706
FaeI CATG 6 cut(s) 307, 364, 466, 567, 625, 864
FatI CATG 6 cut(s) 303, 360, 462, 563, 621, 860
FauI CCCGC 1 cut(s) 937
FbaI TGATCA 1 cut(s) 745
Fnu4HI GCNGC 2 cut(s) 526, 609
FokI GGATG 2 cut(s) 644, 813
FriOI GRGCYC 3 cut(s) 119, 485, 950
Fsp4HI GCNGC 2 cut(s) 526, 609
FspBI CTAG 2 cut(s) 380, 969
GlaI GCGC 1 cut(s) 826
GluI GCNGC 2 cut(s) 526, 609
GsaI CCCAGC 1 cut(s) 405
GsuI CTGGAG 2 cut(s) 276, 765
HaeII RGCGCY 1 cut(s) 828
HaeIII GGCC 2 cut(s) 167, 652
HapII CCGG 2 cut(s) 162, 857
HgaI GACGC 1 cut(s) 440
HhaI GCGC 1 cut(s) 827
Hin1II CATG 6 cut(s) 307, 364, 466, 567, 625, 864
Hin6I GCGC 1 cut(s) 825
HinP1I GCGC 1 cut(s) 825
HinfI GANTC 4 cut(s) 28, 676, 684, 692
HpaII CCGG 2 cut(s) 162, 857
HphI GGTGA 4 cut(s) 40, 403, 436, 883
Hpy166II GTNNAC 2 cut(s) 394, 820
Hpy188I TCNGA 3 cut(s) 257, 455, 697
Hpy188III TCNNGA 8 cut(s) 25, 112, 293, 304, 673, 749, 817, 1034
Hpy8I GTNNAC 2 cut(s) 394, 820
Hpy99I CGWCG 2 cut(s) 155, 556
HpyAV CCTTC 6 cut(s) 61, 109, 369, 722, 791, 880
HpyCH4III ACNGT 2 cut(s) 222, 742
HpyCH4IV ACGT 2 cut(s) 20, 310
HpyCH4V TGCA 6 cut(s) 314, 339, 506, 599, 776, 965
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 605, 962
HpyF3I CTNAG 8 cut(s) 113, 254, 416, 617, 635, 696, 900, 1017
HpySE526I ACGT 2 cut(s) 20, 310
Hsp92II CATG 6 cut(s) 307, 364, 466, 567, 625, 864
HspAI GCGC 1 cut(s) 825
KroI GCCGGC 1 cut(s) 856
KroNI GCCGGC 1 cut(s) 858
Ksp22I TGATCA 1 cut(s) 745
Kzo9I GATC 4 cut(s) 268, 405, 745, 978
LmnI GCTCC 4 cut(s) 43, 122, 853, 995
Lsp1109I GCAGC 2 cut(s) 537, 620
LweI GCATC 2 cut(s) 751, 952
MaeI CTAG 2 cut(s) 380, 969
MaeII ACGT 2 cut(s) 20, 310
MaeIII GTNAC 3 cut(s) 673, 871, 917
MalI GATC 4 cut(s) 270, 407, 747, 980
MboI GATC 4 cut(s) 268, 405, 745, 978
MboII GAAGA 5 cut(s) 46, 82, 220, 440, 988
MflI RGATCY 1 cut(s) 978
MhlI GDGCHC 5 cut(s) 119, 189, 220, 485, 950
MluCI AATT 2 cut(s) 644, 663
MlyI GAGTC 2 cut(s) 670, 701
MnlI CCTC 9 cut(s) 51, 71, 178, 181, 206, 231, 325, 630, 1034
MroNI GCCGGC 1 cut(s) 856
MseI TTAA 1 cut(s) 719
MslI CAYNNNNRTG 1 cut(s) 880
MspI CCGG 2 cut(s) 162, 857
MspR9I CCNGG 2 cut(s) 163, 681
MvaI CCWGG 1 cut(s) 681
MwoI GCNNNNNNNGC 3 cut(s) 224, 605, 962
NaeI GCCGGC 1 cut(s) 858
NciI CCSGG 1 cut(s) 163
NdeII GATC 4 cut(s) 268, 405, 745, 978
NgoMIV GCCGGC 1 cut(s) 856
NlaIII CATG 6 cut(s) 307, 364, 466, 567, 625, 864
NlaIV GGNNCC 3 cut(s) 166, 855, 997
NmuCI GTSAC 2 cut(s) 673, 871
NspI RCATGY 1 cut(s) 625
PagI TCATGA 1 cut(s) 303
PdiI GCCGGC 1 cut(s) 858
PfeI GAWTC 2 cut(s) 28, 684
PfoI TCCNGGA 1 cut(s) 679
PkrI GCNGC 2 cut(s) 527, 610
PleI GAGTC 2 cut(s) 670, 700
PpsI GAGTC 2 cut(s) 670, 700
Ppu21I YACGTR 1 cut(s) 311
PsiI TTATAA 1 cut(s) 594
Psp124BI GAGCTC 1 cut(s) 119
Psp6I CCWGG 1 cut(s) 679
PspFI CCCAGC 1 cut(s) 401
PspGI CCWGG 1 cut(s) 679
PspN4I GGNNCC 3 cut(s) 166, 855, 997
PspPI GGNCC 2 cut(s) 165, 650
PsuI RGATCY 1 cut(s) 978
RsaI GTAC 3 cut(s) 546, 626, 845
RsaNI GTAC 3 cut(s) 545, 625, 844
RseI CAYNNNNRTG 1 cut(s) 880
SacI GAGCTC 1 cut(s) 119
SaqAI TTAA 1 cut(s) 719
SatI GCNGC 2 cut(s) 526, 609
Sau3AI GATC 4 cut(s) 268, 405, 745, 978
Sau96I GGNCC 2 cut(s) 165, 650
SchI GAGTC 2 cut(s) 670, 701
ScrFI CCNGG 2 cut(s) 163, 681
SduI GDGCHC 5 cut(s) 119, 189, 220, 485, 950
SfaNI GCATC 2 cut(s) 751, 952
SmiMI CAYNNNNRTG 1 cut(s) 880
SmlI CTYRAG 1 cut(s) 908
SmoI CTYRAG 1 cut(s) 908
Sse9I AATT 2 cut(s) 644, 663
SsiI CCGC 1 cut(s) 944
SspMI CTAG 2 cut(s) 380, 969
SstI GAGCTC 1 cut(s) 119
StyD4I CCNGG 2 cut(s) 161, 679
StyI CCWWGG 2 cut(s) 324, 706
TaaI ACNGT 2 cut(s) 222, 742
TaiI ACGT 2 cut(s) 23, 313
TaqI TCGA 1 cut(s) 271
TasI AATT 2 cut(s) 644, 663
TfiI GAWTC 2 cut(s) 28, 684
Tru1I TTAA 1 cut(s) 719
Tru9I TTAA 1 cut(s) 719
TscAI CASTG 2 cut(s) 133, 639
TseFI GTSAC 2 cut(s) 673, 871
TseI GCWGC 2 cut(s) 525, 608
Tsp45I GTSAC 2 cut(s) 673, 871
TspDTI ATGAA 4 cut(s) 377, 552, 676, 950
TspGWI ACGGA 1 cut(s) 866
TspRI CASTG 2 cut(s) 133, 639
XapI RAATTY 1 cut(s) 644
XceI RCATGY 1 cut(s) 625
XspI CTAG 2 cut(s) 380, 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.