AT5G52270

25.3 kDa vesicle transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013879
Physical Location & Seq
Reverse (-)
21221432 .. 21222605
1174 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52270.2

Sequence Viewer

Length: 675 bp
ATGGTGAAACTAACAATAGTTGGTAGGGTTGAAGATGGATTGCCTCTTGCACAAGATCAAACCTATGTCAACCAAGAGGACAATACTAGTTTCTTGCTGTACAAGCAACAAGCAGAATTTCTTCTTAAACAAGTCTCCAAAGACTCATTATTACATCCAAAGATGACCATCTTGCTCGATCATCATTCTTTCCACTTCCTGGTGGAGAAGAAGATATGTTACATCGCGCTATCTGATTCTTCATATCCAAGAAAGCTATTGTTTAATTACTTGCAGAATCTGAACAAGGAGCTTGATAAGCTGGACGAGAAAGCACTGATCCAGAAAATCTCAAAGCCCTATAGCTTCATTAGGTTTGGTAAGATCATAGGGAGAATAAGAAAACAATATATAGACACGAGAACACAAGCTAATCTATCGAAGCTGAATGCATTGCGGAAACAAGAACTCGATGTAGTTACTGAGCATTTGAATGATATAATACAAAGACAACAAATTTTAGAAAAGGCGTCCTCAGATCCTCCAATGATTGTTTCAACCATTTGGAGCTCACGATGTCTTCAGGATATTTCGTTAAAATGGACACCAGTGACGATTATTATTCTCGTTATTCTTGTTCTTTTCAAAGCAAGCTTGATTATGACAGATGATTATTTAATTTCAACCATGATATGA

Protein Analysis

224

Amino Acids

26.01

Weight (kDa)

9.07

Isoelectric Point (pI)

29.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Longin PF13774 39 - 120 3.1e-15 Regulated-SNARE-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 199
AccII CGCG 1 cut(s) 227
AciI CCGC 1 cut(s) 436
AclWI GGATC 2 cut(s) 313, 512
AcsI RAATTY 2 cut(s) 116, 495
AcuI CTGAAG 1 cut(s) 545
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 199
AgsI TTSAA 5 cut(s) 32, 472, 537, 625, 663
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 1 cut(s) 198
AjuI GAANNNNNNNTTGG 2 cut(s) 517, 549
AluBI AGCT 8 cut(s) 256, 292, 301, 345, 410, 424, 549, 633
AluI AGCT 8 cut(s) 256, 292, 301, 345, 410, 424, 549, 633
Alw21I GWGCWC 1 cut(s) 551
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 2 cut(s) 313, 512
AlwNI CAGNNNCTG 1 cut(s) 280
ApoI RAATTY 2 cut(s) 116, 495
Asp700I GAANNNNTTC 1 cut(s) 120
AspLEI GCGC 1 cut(s) 229
AsuHPI GGTGA 1 cut(s) 16
BanII GRGCYC 1 cut(s) 551
BarI GAAGNNNNNNTAC 2 cut(s) 203, 235
BauI CACGAG 1 cut(s) 397
BbsI GAAGAC 1 cut(s) 551
Bbv12I GWGCWC 1 cut(s) 551
BccI CCATC 2 cut(s) 29, 176
BciT130I CCWGG 1 cut(s) 200
BcoDI GTCTC 1 cut(s) 139
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 1 cut(s) 87
BfmI CTRYAG 1 cut(s) 340
Bme1390I CCNGG 1 cut(s) 200
BmrFI CCNGG 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 551
BsaBI GATNNNNATC 1 cut(s) 167
BsaHI GRCGYC 1 cut(s) 509
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 1 cut(s) 587
Bse3DI GCAATG 1 cut(s) 431
Bse8I GATNNNNATC 1 cut(s) 167
BseBI CCWGG 1 cut(s) 200
BseGI GGATG 1 cut(s) 154
BseJI GATNNNNATC 1 cut(s) 167
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMI GCAATG 1 cut(s) 431
BseMII CTCAG 2 cut(s) 453, 528
BseNI ACTGG 1 cut(s) 587
Bsh1236I CGCG 1 cut(s) 227
BsiHKAI GWGCWC 1 cut(s) 551
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 1 cut(s) 139
BsmI GAATGC 1 cut(s) 433
Bsp1286I GDGCHC 1 cut(s) 551
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 5 cut(s) 55, 178, 318, 363, 517
BspACI CCGC 1 cut(s) 436
BspCNI CTCAG 2 cut(s) 454, 527
BspFNI CGCG 1 cut(s) 227
BspPI GGATC 2 cut(s) 313, 512
BsrDI GCAATG 1 cut(s) 431
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 1 cut(s) 587
BssMI GATC 5 cut(s) 55, 178, 318, 363, 517
BssNI GRCGYC 1 cut(s) 509
BssSI CACGAG 1 cut(s) 397
Bst2BI CACGAG 1 cut(s) 397
Bst2UI CCWGG 1 cut(s) 200
BstACI GRCGYC 1 cut(s) 509
BstAUI TGTACA 1 cut(s) 99
BstC8I GCNNGC 1 cut(s) 631
BstDEI CTNAG 2 cut(s) 462, 514
BstF5I GGATG 1 cut(s) 154
BstFNI CGCG 1 cut(s) 227
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 5 cut(s) 58, 181, 321, 366, 520
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 5 cut(s) 55, 178, 318, 363, 517
BstMWI GCNNNNNNNGC 2 cut(s) 103, 298
BstNI CCWGG 1 cut(s) 200
BstSCI CCNGG 1 cut(s) 198
BstSFI CTRYAG 1 cut(s) 340
BstUI CGCG 1 cut(s) 227
BstV2I GAAGAC 1 cut(s) 551
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
BtgZI GCGATG 1 cut(s) 208
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 2 cut(s) 314, 594
Cac8I GCNNGC 1 cut(s) 631
CaiI CAGNNNCTG 1 cut(s) 280
CfoI GCGC 1 cut(s) 229
CseI GACGC 1 cut(s) 498
Csp6I GTAC 1 cut(s) 100
CviAII CATG 1 cut(s) 667
CviJI RGCY 9 cut(s) 256, 292, 301, 337, 345, 410, 424, 549, 633
CviKI_1 RGCY 9 cut(s) 256, 292, 301, 337, 345, 410, 424, 549, 633
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 2 cut(s) 462, 514
DpnI GATC 5 cut(s) 57, 180, 320, 365, 519
DpnII GATC 5 cut(s) 55, 178, 318, 363, 517
Ecl136II GAGCTC 1 cut(s) 549
Eco24I GRGCYC 1 cut(s) 551
Eco53kI GAGCTC 1 cut(s) 549
Eco57I CTGAAG 1 cut(s) 545
EcoICRI GAGCTC 1 cut(s) 549
EcoRII CCWGG 1 cut(s) 198
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 551
FaeI CATG 1 cut(s) 670
FatI CATG 1 cut(s) 666
FokI GGATG 1 cut(s) 141
FriOI GRGCYC 1 cut(s) 551
FspBI CTAG 1 cut(s) 87
GlaI GCGC 1 cut(s) 228
HgaI GACGC 1 cut(s) 498
HhaI GCGC 1 cut(s) 229
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 1 cut(s) 670
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HindIII AAGCTT 1 cut(s) 631
HinfI GANTC 3 cut(s) 143, 236, 277
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 3 cut(s) 235, 282, 517
Hpy188III TCNNGA 3 cut(s) 322, 552, 563
Hpy8I GTNNAC 1 cut(s) 70
HpyCH4V TGCA 3 cut(s) 50, 274, 431
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 298
HpyF3I CTNAG 2 cut(s) 462, 514
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 1 cut(s) 670
HspAI GCGC 1 cut(s) 227
Kzo9I GATC 5 cut(s) 55, 178, 318, 363, 517
LmnI GCTCC 2 cut(s) 289, 546
LpnPI CCDG 6 cut(s) 185, 212, 287, 335, 548, 600
MaeI CTAG 1 cut(s) 87
MaeIII GTNAC 3 cut(s) 218, 457, 589
MalI GATC 5 cut(s) 57, 180, 320, 365, 519
MboI GATC 5 cut(s) 55, 178, 318, 363, 517
MboII GAAGA 6 cut(s) 44, 113, 220, 223, 231, 551
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 1 cut(s) 551
MluCI AATT 4 cut(s) 116, 265, 495, 657
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 4 cut(s) 54, 70, 523, 531
Mph1103I ATGCAT 1 cut(s) 433
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 4 cut(s) 126, 264, 575, 656
MslI CAYNNNNRTG 1 cut(s) 471
MspR9I CCNGG 1 cut(s) 200
Mva1269I GAATGC 1 cut(s) 433
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 2 cut(s) 103, 298
NdeII GATC 5 cut(s) 55, 178, 318, 363, 517
NlaIII CATG 1 cut(s) 670
NmuCI GTSAC 1 cut(s) 589
NsiI ATGCAT 1 cut(s) 433
PctI GAATGC 1 cut(s) 433
PdmI GAANNNNTTC 1 cut(s) 120
PfeI GAWTC 2 cut(s) 236, 277
PflMI CCANNNNNTGG 1 cut(s) 199
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 551
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PstNI CAGNNNCTG 1 cut(s) 280
PsuI RGATCY 1 cut(s) 517
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 471
SacI GAGCTC 1 cut(s) 551
SaqAI TTAA 4 cut(s) 126, 264, 575, 656
Sau3AI GATC 5 cut(s) 55, 178, 318, 363, 517
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 1 cut(s) 551
SfcI CTRYAG 1 cut(s) 340
SmiMI CAYNNNNRTG 1 cut(s) 471
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 4 cut(s) 116, 265, 495, 657
SsiI CCGC 1 cut(s) 436
SspMI CTAG 1 cut(s) 87
SstI GAGCTC 1 cut(s) 551
StyD4I CCNGG 1 cut(s) 198
TaqI TCGA 3 cut(s) 177, 419, 450
TasI AATT 4 cut(s) 116, 265, 495, 657
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 2 cut(s) 236, 277
Tru1I TTAA 4 cut(s) 126, 264, 575, 656
Tru9I TTAA 4 cut(s) 126, 264, 575, 656
TscAI CASTG 2 cut(s) 321, 594
TseFI GTSAC 1 cut(s) 589
Tsp45I GTSAC 1 cut(s) 589
TspDTI ATGAA 2 cut(s) 231, 337
TspRI CASTG 2 cut(s) 321, 594
Van91I CCANNNNNTGG 1 cut(s) 199
XapI RAATTY 2 cut(s) 116, 495
XmnI GAANNNNTTC 1 cut(s) 120
XspI CTAG 1 cut(s) 87
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.