Rh4AG111000

Non-structural maintenance of chromosomes element 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0013879
Physical Location & Seq
Reverse (-)
23980512 .. 24007262
26751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG111000.1

Sequence Viewer

Length: 615 bp
ATGCTGAAGTCAAAAGGCAGGGCCGAAAACCAACTGCATGGAAAAACTGTGCGATTTGGGGGTTTGACGAGTGGTAAACTAAAAGCAGAGGAGGCACCCAAGAACATTGAGCACCAAATCGAAGGGACCCAGAATGATACATTCCATTATGGTCTTAATAGCGTCAAAAGCAACCTCATTGGCTTTCATGCAGTCGAATCTGCTCACCAATTGATTACAAATAACATGCTTTGTTGGTTAGTAGCACATTTCAATACTCATTTCACTTTCCTGATCGACACGAATGAAGAAGAAACTCAAATTTTCAATGGTTGCAAATTTCAGAGGCCTACTGGAGCCATACCTTCTTCAATGCAGATAGAAGCAATAGTGCAAGAAGCTGCTCAAGGAACCATATCTAGCAGTGACACTCTTAATCTACGATTAGAGAATCAGGTTAACAGTGGTACATCATCACAATCACAAGGAGGTTCACTTCCTATCCCTACTGCATTGAAGAACTTTTCAATGTCCCAGCAGGGAAAAACTCTTGATGAATTTGTACGGGATAAATGGCTTTCCTTCACTGCTGAGGGTCATGTTGGACTTTGTGTCAGATCCTTCCTTGATCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMC_Nse1 zf-RING-like

Protein Analysis

204

Amino Acids

22.47

Weight (kDa)

6.23

Isoelectric Point (pI)

32.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_Nse1 PF07574 120 - 204 9.1e-10 Nse1 non-SMC component of SMC5-6 complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 94
AclWI GGATC 1 cut(s) 591
AcsI RAATTY 3 cut(s) 300, 317, 536
AcuI CTGAAG 1 cut(s) 26
AfaI GTAC 2 cut(s) 448, 543
AgsI TTSAA 5 cut(s) 253, 307, 351, 496, 507
AhdI GACNNNNNGTC 1 cut(s) 590
AluBI AGCT 1 cut(s) 380
AluI AGCT 1 cut(s) 380
Alw21I GWGCWC 1 cut(s) 114
AlwI GGATC 1 cut(s) 591
AoxI GGCC 2 cut(s) 21, 326
ApeKI GCWGC 1 cut(s) 380
ApoI RAATTY 3 cut(s) 300, 317, 536
AspS9I GGNCC 2 cut(s) 21, 126
AsuHPI GGTGA 1 cut(s) 197
AvaII GGWCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 114
BbvCI CCTCAGC 1 cut(s) 570
BbvI GCAGC 1 cut(s) 367
BfaI CTAG 1 cut(s) 399
BisI GCNGC 1 cut(s) 381
BlsI GCNGC 1 cut(s) 382
Bme18I GGWCC 1 cut(s) 126
BmeRI GACNNNNNGTC 1 cut(s) 590
BmgT120I GGNCC 2 cut(s) 21, 126
BmiI GGNNCC 5 cut(s) 96, 127, 128, 337, 391
BpmI CTGGAG 1 cut(s) 354
Bpu10I CCTNAGC 1 cut(s) 570
BpuEI CTTGAG 1 cut(s) 369
Bse1I ACTGG 1 cut(s) 337
BseMII CTCAG 1 cut(s) 561
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 1 cut(s) 367
BseYI CCCAGC 1 cut(s) 513
BshFI GGCC 2 cut(s) 23, 328
BshNI GGYRCC 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 114
BslFI GGGAC 2 cut(s) 139, 496
BsmFI GGGAC 2 cut(s) 139, 496
BsnI GGCC 2 cut(s) 23, 328
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 3 cut(s) 273, 596, 607
BspANI GGCC 2 cut(s) 23, 328
BspCNI CTCAG 1 cut(s) 562
BspLI GGNNCC 5 cut(s) 96, 127, 128, 337, 391
BspPI GGATC 1 cut(s) 591
BspT107I GGYRCC 1 cut(s) 94
BsrI ACTGG 1 cut(s) 337
BssMI GATC 3 cut(s) 273, 596, 607
Bst4CI ACNGT 2 cut(s) 49, 443
BstDEI CTNAG 1 cut(s) 570
BstKTI GATC 3 cut(s) 276, 599, 610
BstMBI GATC 3 cut(s) 273, 596, 607
BstMWI GCNNNNNNNGC 2 cut(s) 92, 168
BstNSI RCATGY 1 cut(s) 229
BstV1I GCAGC 1 cut(s) 367
BstX2I RGATCY 1 cut(s) 596
BstXI CCANNNNNNTGG 1 cut(s) 38
BstYI RGATCY 1 cut(s) 596
BsuRI GGCC 2 cut(s) 23, 328
BtsI GCAGTG 2 cut(s) 409, 564
BtsIMutI CAGTG 3 cut(s) 409, 448, 564
Cfr13I GGNCC 2 cut(s) 21, 126
CseI GACGC 1 cut(s) 151
Csp6I GTAC 2 cut(s) 447, 542
CviAII CATG 4 cut(s) 38, 188, 226, 578
CviJI RGCY 6 cut(s) 23, 183, 328, 338, 380, 556
CviKI_1 RGCY 6 cut(s) 23, 183, 328, 338, 380, 556
CviQI GTAC 2 cut(s) 447, 542
DdeI CTNAG 1 cut(s) 570
DpnI GATC 3 cut(s) 275, 598, 609
DpnII GATC 3 cut(s) 273, 596, 607
DriI GACNNNNNGTC 1 cut(s) 590
Eam1105I GACNNNNNGTC 1 cut(s) 590
Eco147I AGGCCT 1 cut(s) 328
Eco47I GGWCC 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 126
FaeI CATG 4 cut(s) 41, 191, 229, 581
FaiI YATR 7 cut(s) 39, 150, 189, 227, 341, 395, 579
FaqI GGGAC 2 cut(s) 139, 496
FatI CATG 4 cut(s) 37, 187, 225, 577
Fnu4HI GCNGC 1 cut(s) 381
Fsp4HI GCNGC 1 cut(s) 381
FspBI CTAG 1 cut(s) 399
GluI GCNGC 1 cut(s) 381
GsaI CCCAGC 1 cut(s) 517
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 2 cut(s) 23, 328
HgaI GACGC 1 cut(s) 151
Hin1II CATG 4 cut(s) 41, 191, 229, 581
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 2 cut(s) 197, 430
HpaI GTTAAC 1 cut(s) 439
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 3 cut(s) 77, 439, 473
Hpy188I TCNGA 2 cut(s) 324, 596
Hpy188III TCNNGA 2 cut(s) 271, 530
Hpy8I GTNNAC 3 cut(s) 77, 439, 473
HpyAV CCTTC 4 cut(s) 116, 354, 571, 610
HpyCH4III ACNGT 2 cut(s) 49, 443
HpyCH4V TGCA 6 cut(s) 37, 191, 315, 355, 373, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 168
HpyF3I CTNAG 1 cut(s) 570
Hsp92II CATG 4 cut(s) 41, 191, 229, 581
KflI GGGWCCC 1 cut(s) 126
KspAI GTTAAC 1 cut(s) 439
Kzo9I GATC 3 cut(s) 273, 596, 607
LmnI GCTCC 1 cut(s) 335
LpnPI CCDG 7 cut(s) 4, 143, 284, 318, 419, 503, 527
Lsp1109I GCAGC 1 cut(s) 367
MaeI CTAG 1 cut(s) 399
MaeIII GTNAC 1 cut(s) 404
MalI GATC 3 cut(s) 275, 598, 609
MboI GATC 3 cut(s) 273, 596, 607
MboII GAAGA 4 cut(s) 299, 302, 339, 508
MfeI CAATTG 1 cut(s) 209
MflI RGATCY 1 cut(s) 596
MhlI GDGCHC 1 cut(s) 114
MluCI AATT 4 cut(s) 209, 300, 317, 536
MmeI TCCRAC 1 cut(s) 562
MnlI CCTC 6 cut(s) 82, 85, 185, 318, 461, 565
MseI TTAA 3 cut(s) 156, 414, 438
MunI CAATTG 1 cut(s) 209
MwoI GCNNNNNNNGC 2 cut(s) 92, 168
NdeII GATC 3 cut(s) 273, 596, 607
NlaIII CATG 4 cut(s) 41, 191, 229, 581
NlaIV GGNNCC 5 cut(s) 96, 127, 128, 337, 391
NmuCI GTSAC 1 cut(s) 404
NspI RCATGY 1 cut(s) 229
PceI AGGCCT 1 cut(s) 328
PfeI GAWTC 2 cut(s) 197, 430
PkrI GCNGC 1 cut(s) 382
PpuMI RGGWCCY 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 126
PspFI CCCAGC 1 cut(s) 513
PspN4I GGNNCC 5 cut(s) 96, 127, 128, 337, 391
PspPI GGNCC 2 cut(s) 21, 126
PspPPI RGGWCCY 1 cut(s) 126
PsuI RGATCY 1 cut(s) 596
RsaI GTAC 2 cut(s) 448, 543
RsaNI GTAC 2 cut(s) 447, 542
SaqAI TTAA 3 cut(s) 156, 414, 438
SatI GCNGC 1 cut(s) 381
Sau3AI GATC 3 cut(s) 273, 596, 607
Sau96I GGNCC 2 cut(s) 21, 126
SduI GDGCHC 1 cut(s) 114
SetI ASST 5 cut(s) 177, 346, 382, 438, 472
SinI GGWCC 1 cut(s) 126
SmlI CTYRAG 1 cut(s) 384
SmoI CTYRAG 1 cut(s) 384
Sse9I AATT 4 cut(s) 209, 300, 317, 536
SseBI AGGCCT 1 cut(s) 328
SspMI CTAG 1 cut(s) 399
StuI AGGCCT 1 cut(s) 328
TaaI ACNGT 2 cut(s) 49, 443
TaqI TCGA 3 cut(s) 120, 195, 276
TasI AATT 4 cut(s) 209, 300, 317, 536
TfiI GAWTC 2 cut(s) 197, 430
Tru1I TTAA 3 cut(s) 156, 414, 438
Tru9I TTAA 3 cut(s) 156, 414, 438
TscAI CASTG 3 cut(s) 409, 448, 571
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 1 cut(s) 380
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 3 cut(s) 176, 300, 549
TspRI CASTG 3 cut(s) 409, 448, 571
VpaK11BI GGWCC 1 cut(s) 126
XapI RAATTY 3 cut(s) 300, 317, 536
XceI RCATGY 1 cut(s) 229
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.