MD01G1200300.v1.1

Belongs to the synaptobrevin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013879
Physical Location & Seq
Reverse (-)
29714826 .. 29715906
1081 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1200300.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGTCAAGCTTACAATTGTTGGAAGGGTAAGTGATAGCCTTTGTCTTGCCAAAGGACCGTCCTATATGAACGAAGAGCGCGAAAACTCTTCATTTTACAAGCAACAAGGAGAGTTCATATTCAAAGAAATCTCCAGAGGAGCCTTGCCTCCTTCCAGGATGACCATTCGTGTCGATCATCACTGCTTCAATTACTTGGTGGAGAAGGGAATCGCCTTCATCACGTTGTGTGAATCTTCATATCCAAGAAAACTAGCTTTCTGTTACCTGCAAGAATTGCAGAAGGAATTTGACAAGTTTGACAGTAGCCTTATTGACAAAATCATAAGACCGTACTCCTTCGTCAAATTCGATGGTGTAATTGGGAGTACAAGAAAGCAATACATCGATACAAGAACACAGGTTAATCTGTCTAAGCTTAATGCTAACCGGAAACAAGATTTAGACATTGCCACTGAAAATCTTTCAGAAATCATAGAGAGATGGAGACATTCAGGTAACATCCATTTCATCTTTATTGCTACCTTCATCGCTAAGGCATGCATGAACACATCATCTGCTTCCCTCTTTTGTTCTAAAATCTTAAAATTACAGATCTATTGGAAAGGCCATCGACTCCTCCTCCTCCTCCTCCTCCTCCTCAAGCTGTTTCATCCATTGGGTTTTCACCGCTCCTCGAGGTTATTGCATTGA

Protein Analysis

231

Amino Acids

26.72

Weight (kDa)

9.51

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Longin PF13774 40 - 118 1e-21 Regulated-SNARE-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 676
Acc36I ACCTGC 1 cut(s) 276
AccBSI CCGCTC 1 cut(s) 672
AccII CGCG 1 cut(s) 81
AciI CCGC 1 cut(s) 670
AcsI RAATTY 2 cut(s) 287, 347
AdeI CACNNNGTG 1 cut(s) 228
AfaI GTAC 2 cut(s) 335, 370
AgsI TTSAA 2 cut(s) 124, 190
AjnI CCWGG 1 cut(s) 155
AluBI AGCT 4 cut(s) 10, 257, 418, 646
AluI AGCT 4 cut(s) 10, 257, 418, 646
Alw26I GTCTC 1 cut(s) 481
Ama87I CYCGRG 1 cut(s) 676
AoxI GGCC 1 cut(s) 607
ApoI RAATTY 2 cut(s) 287, 347
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 56
AsuHPI GGTGA 1 cut(s) 659
AvaI CYCGRG 1 cut(s) 676
AvaII GGWCC 1 cut(s) 56
BccI CCATC 3 cut(s) 347, 477, 618
BcgI CGANNNNNNTGC 1 cut(s) 667
BciT130I CCWGG 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 481
BfaI CTAG 1 cut(s) 254
BfuAI ACCTGC 1 cut(s) 276
BglII AGATCT 1 cut(s) 594
Bme1390I CCNGG 1 cut(s) 157
Bme18I GGWCC 1 cut(s) 56
BmeT110I CYCGRG 1 cut(s) 676
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 1 cut(s) 157
BpmI CTGGAG 1 cut(s) 118
Bpu10I CCTNAGC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 626
Bsa29I ATCGAT 1 cut(s) 387
BsaWI WCCGGW 1 cut(s) 429
BsaXI ACNNNNNCTCC 2 cut(s) 606, 636
Bse3DI GCAATG 1 cut(s) 447
BseBI CCWGG 1 cut(s) 157
BseCI ATCGAT 1 cut(s) 387
BseGI GGATG 3 cut(s) 165, 501, 652
BseMI GCAATG 1 cut(s) 447
Bsh1236I CGCG 1 cut(s) 81
BshFI GGCC 1 cut(s) 609
BshVI ATCGAT 1 cut(s) 387
BsiHKCI CYCGRG 1 cut(s) 676
BsiSI CCGG 1 cut(s) 430
BsmAI GTCTC 1 cut(s) 481
BsnI GGCC 1 cut(s) 609
BsoBI CYCGRG 1 cut(s) 676
Bsp143I GATC 2 cut(s) 175, 594
BspACI CCGC 1 cut(s) 670
BspANI GGCC 1 cut(s) 609
BspDI ATCGAT 1 cut(s) 387
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 142
BspMI ACCTGC 1 cut(s) 276
BspQI GCTCTTC 1 cut(s) 69
BsrBI CCGCTC 1 cut(s) 672
BsrDI GCAATG 1 cut(s) 447
BssMI GATC 2 cut(s) 175, 594
Bst2UI CCWGG 1 cut(s) 157
Bst4CI ACNGT 3 cut(s) 60, 305, 333
Bst6I CTCTTC 2 cut(s) 69, 94
BstAPI GCANNNNNTGC 1 cut(s) 277
BstC8I GCNNGC 1 cut(s) 541
BstDEI CTNAG 2 cut(s) 414, 534
BstF5I GGATG 3 cut(s) 165, 501, 652
BstFNI CGCG 1 cut(s) 81
BstHHI GCGC 1 cut(s) 81
BstKTI GATC 2 cut(s) 178, 597
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 2 cut(s) 175, 594
BstMWI GCNNNNNNNGC 1 cut(s) 277
BstNI CCWGG 1 cut(s) 157
BstNSI RCATGY 1 cut(s) 543
BstSCI CCNGG 1 cut(s) 155
BstUI CGCG 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 594
BstYI RGATCY 1 cut(s) 594
Bsu15I ATCGAT 1 cut(s) 387
BsuRI GGCC 1 cut(s) 609
BsuTUI ATCGAT 1 cut(s) 387
BtgZI GCGATG 1 cut(s) 514
BtsCI GGATG 3 cut(s) 165, 501, 652
BtsI GCAGTG 1 cut(s) 181
BtsIMutI CAGTG 2 cut(s) 181, 453
BveI ACCTGC 1 cut(s) 276
Cac8I GCNNGC 1 cut(s) 541
CfoI GCGC 1 cut(s) 81
Cfr13I GGNCC 1 cut(s) 56
ClaI ATCGAT 1 cut(s) 387
Csp6I GTAC 2 cut(s) 334, 369
CviAII CATG 2 cut(s) 540, 544
CviJI RGCY 8 cut(s) 10, 39, 143, 257, 309, 418, 609, 646
CviKI_1 RGCY 8 cut(s) 10, 39, 143, 257, 309, 418, 609, 646
CviQI GTAC 2 cut(s) 334, 369
DdeI CTNAG 2 cut(s) 414, 534
DpnI GATC 2 cut(s) 177, 596
DpnII GATC 2 cut(s) 175, 594
DraIII CACNNNGTG 1 cut(s) 228
Eam1104I CTCTTC 2 cut(s) 69, 94
EarI CTCTTC 2 cut(s) 69, 94
Eco47I GGWCC 1 cut(s) 56
Eco88I CYCGRG 1 cut(s) 676
EcoRII CCWGG 1 cut(s) 155
EcoT22I ATGCAT 1 cut(s) 545
FaeI CATG 2 cut(s) 543, 547
FaiI YATR 8 cut(s) 66, 68, 119, 241, 326, 476, 541, 545
FatI CATG 2 cut(s) 539, 543
FokI GGATG 3 cut(s) 172, 488, 639
FspBI CTAG 1 cut(s) 254
GlaI GCGC 1 cut(s) 80
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 1 cut(s) 609
HapII CCGG 1 cut(s) 430
HhaI GCGC 1 cut(s) 81
Hin1II CATG 2 cut(s) 543, 547
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HindIII AAGCTT 2 cut(s) 8, 416
HinfI GANTC 3 cut(s) 210, 233, 615
HpaII CCGG 1 cut(s) 430
HphI GGTGA 1 cut(s) 659
Hpy188I TCNGA 1 cut(s) 469
Hpy188III TCNNGA 1 cut(s) 135
HpyAV CCTTC 7 cut(s) 18, 162, 199, 226, 277, 349, 535
HpyCH4III ACNGT 3 cut(s) 60, 305, 333
HpyCH4IV ACGT 1 cut(s) 224
HpyCH4V TGCA 4 cut(s) 271, 280, 543, 688
HpyF10VI GCNNNNNNNGC 1 cut(s) 277
HpyF3I CTNAG 2 cut(s) 414, 534
HpySE526I ACGT 1 cut(s) 224
Hsp92II CATG 2 cut(s) 543, 547
HspAI GCGC 1 cut(s) 79
Kzo9I GATC 2 cut(s) 175, 594
LguI GCTCTTC 1 cut(s) 69
LmnI GCTCC 2 cut(s) 140, 677
LpnPI CCDG 7 cut(s) 142, 148, 169, 281, 386, 443, 480
MaeI CTAG 1 cut(s) 254
MaeII ACGT 1 cut(s) 224
MaeIII GTNAC 2 cut(s) 263, 497
MalI GATC 2 cut(s) 177, 596
MbiI CCGCTC 1 cut(s) 672
MboI GATC 2 cut(s) 175, 594
MboII GAAGA 3 cut(s) 81, 86, 228
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 1 cut(s) 594
MluCI AATT 7 cut(s) 15, 190, 275, 287, 347, 360, 587
MlyI GAGTC 1 cut(s) 609
Mph1103I ATGCAT 1 cut(s) 545
MseI TTAA 3 cut(s) 405, 420, 584
MspI CCGG 1 cut(s) 430
MspR9I CCNGG 1 cut(s) 157
MunI CAATTG 1 cut(s) 15
MvaI CCWGG 1 cut(s) 157
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 1 cut(s) 277
NdeII GATC 2 cut(s) 175, 594
NlaIII CATG 2 cut(s) 543, 547
NlaIV GGNNCC 1 cut(s) 142
NsiI ATGCAT 1 cut(s) 545
NspI RCATGY 1 cut(s) 543
PaeI GCATGC 1 cut(s) 543
PaeR7I CTCGAG 1 cut(s) 676
PciSI GCTCTTC 1 cut(s) 69
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 348
PfeI GAWTC 2 cut(s) 210, 233
PfoI TCCNGGA 1 cut(s) 155
PleI GAGTC 1 cut(s) 609
PpsI GAGTC 1 cut(s) 609
Psp6I CCWGG 1 cut(s) 155
PspGI CCWGG 1 cut(s) 155
PspN4I GGNNCC 1 cut(s) 142
PspPI GGNCC 1 cut(s) 56
PspXI VCTCGAGB 1 cut(s) 676
PsuI RGATCY 1 cut(s) 594
RsaI GTAC 2 cut(s) 335, 370
RsaNI GTAC 2 cut(s) 334, 369
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 3 cut(s) 405, 420, 584
Sau3AI GATC 2 cut(s) 175, 594
Sau96I GGNCC 1 cut(s) 56
SchI GAGTC 1 cut(s) 609
ScrFI CCNGG 1 cut(s) 157
Sfr274I CTCGAG 1 cut(s) 676
SinI GGWCC 1 cut(s) 56
SlaI CTCGAG 1 cut(s) 676
SmlI CTYRAG 2 cut(s) 641, 676
SmoI CTYRAG 2 cut(s) 641, 676
SphI GCATGC 1 cut(s) 543
Sse9I AATT 7 cut(s) 15, 190, 275, 287, 347, 360, 587
SsiI CCGC 1 cut(s) 670
SspMI CTAG 1 cut(s) 254
StyD4I CCNGG 1 cut(s) 155
TaaI ACNGT 3 cut(s) 60, 305, 333
TaiI ACGT 1 cut(s) 227
TaqI TCGA 5 cut(s) 174, 351, 387, 613, 677
TasI AATT 7 cut(s) 15, 190, 275, 287, 347, 360, 587
TatI WGTACW 1 cut(s) 368
TfiI GAWTC 2 cut(s) 210, 233
Tru1I TTAA 3 cut(s) 405, 420, 584
Tru9I TTAA 3 cut(s) 405, 420, 584
TscAI CASTG 2 cut(s) 188, 460
TspDTI ATGAA 9 cut(s) 81, 83, 106, 208, 228, 499, 517, 560, 641
TspRI CASTG 2 cut(s) 188, 460
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 2 cut(s) 287, 347
XceI RCATGY 1 cut(s) 543
XhoI CTCGAG 1 cut(s) 676
XspI CTAG 1 cut(s) 254
Zsp2I ATGCAT 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.