Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0013879

Physical Location & Seq

Genomic Coordinates Reverse (-)

33589580 .. 33590685

Gene Structure 1106 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1268100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 678 bp

ATGGTAAAGCTAACAATTGTTGGAAGGGTAAGTGATGGACTTTGTCTTGCCAAAGGACCGTCTTATATGAATGAAGAGCACGAAAACTCTTCGTTTTACAAGCAACAAGGAGAGTTCATATTCAAAGAAATCTCCAGAGGAGCCTTGCCTCCTTCCAGGATGACCATTCGTGTCGATCATCACTGCTTCAATTACTTGGTGGAGAAGGGAATTGCATTCATCGCGTTGTGCGAATCTTCGTATCCAAGAAAACTAGCTTTCCATTACCTTCAAGATTTGCAGAAGGAATTTGAAAAGTTCGACAGTAACCTCATTAACAAAATCACAAGACCATACTCCTTCGTCAAATTCGATGGTGTCATTGGGAAAATTAGAAAACAATACATTGATACAAGAACACAGGTTAATCTGTCGAAGCTTAATGCTAACCGGAAACATGATTTAGACATTGCTACTGAAAATCTTTCAGAAATCATAGAGAGACGGAGACATTCAGATCTATTGGAAAGACCATTGCTTCCTCCTCCTCCTCAAGCTGTTTCATCTATTTGGTGTTCACCACTCCTCGAGGTTATTGCGTTGAAATGGACACCTATTGCCTTGATAATTGCTGTTGCCACTGTTGTTCTTTGGGTCTCCATAATCCATGCAGACAACAATTTCATGATTCTAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005886 GO:0006810 GO:0006887 GO:0006906 GO:0006996 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031201 GO:0032940 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048284 GO:0051179 GO:0051234 GO:0061024 GO:0061025 GO:0071840 GO:0071944 GO:0090174 GO:0098796

KEGG Pathways

Metabolic & Signaling

ko04130 ko04145 ko05134 map04130 map04145 map05134

Pfam Domains

Protein Families

Longin

Protein Analysis

226

Amino Acids

25.84

Weight (kDa)

8.83

Isoelectric Point (pI)

46.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Longin	PF13774	40 - 118	9.6e-22	Regulated-SNARE-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013879)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52270 AT5G52270
fragaria_vesca	FvH4_6g47281
malus_domestica	MD01G1200300.v1.1 MD07G1268100.v1.1
prunus_persica	Prupe.2G293800_v2.0.a1
pyrus_communis	pycom07g24570
rosa_chinensis	RchiOBHm_Chr1g0333651 RchiOBHm_Chr5g0053861
rosa_roxburghii	Rroxscaffold_4G00317350
rosa_rugosa	Rorug02G0522400 Rorug06G0275000 Rorug07G0304600
rosa_samantha	Rh4AG111000 Rh6DG154600 Rh7AG307300 Rh7CG326300

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	566
AccII	CGCG	1 cut(s)	224
AcsI	RAATTY	2 cut(s)	287, 347
AgsI	TTSAA	5 cut(s)	124, 190, 272, 293, 583
AjnI	CCWGG	1 cut(s)	155
AluBI	AGCT	4 cut(s)	10, 257, 418, 536
AluI	AGCT	4 cut(s)	10, 257, 418, 536
Alw21I	GWGCWC	1 cut(s)	81
Alw26I	GTCTC	3 cut(s)	475, 481, 640
Ama87I	CYCGRG	1 cut(s)	566
ApoI	RAATTY	2 cut(s)	287, 347
AspS9I	GGNCC	1 cut(s)	56
AsuHPI	GGTGA	1 cut(s)	549
AvaI	CYCGRG	1 cut(s)	566
AvaII	GGWCC	1 cut(s)	56
Bbv12I	GWGCWC	1 cut(s)	81
BccI	CCATC	2 cut(s)	29, 347
BcgI	CGANNNNNNTGC	2 cut(s)	557, 591
BciT130I	CCWGG	1 cut(s)	157
BciVI	GTATCC	1 cut(s)	252
BcoDI	GTCTC	3 cut(s)	475, 481, 640
BfaI	CTAG	2 cut(s)	254, 676
BfuI	GTATCC	1 cut(s)	252
BglII	AGATCT	1 cut(s)	496
Bme1390I	CCNGG	1 cut(s)	157
Bme18I	GGWCC	1 cut(s)	56
BmeT110I	CYCGRG	1 cut(s)	566
BmgT120I	GGNCC	1 cut(s)	56
BmiI	GGNNCC	1 cut(s)	142
BmrFI	CCNGG	1 cut(s)	157
BpmI	CTGGAG	1 cut(s)	118
BpuEI	CTTGAG	1 cut(s)	516
BsaI	GGTCTC	1 cut(s)	640
BsaWI	WCCGGW	1 cut(s)	429
Bse3DI	GCAATG	2 cut(s)	447, 512
BseBI	CCWGG	1 cut(s)	157
BseGI	GGATG	1 cut(s)	165
BseMI	GCAATG	2 cut(s)	447, 512
BseRI	GAGGAG	5 cut(s)	153, 513, 516, 519, 554
Bsh1236I	CGCG	1 cut(s)	224
BsiHKAI	GWGCWC	1 cut(s)	81
BsiHKCI	CYCGRG	1 cut(s)	566
BsiSI	CCGG	1 cut(s)	430
BsmAI	GTCTC	3 cut(s)	475, 481, 640
BsmBI	CGTCTC	1 cut(s)	475
BsmI	GAATGC	1 cut(s)	215
Bso31I	GGTCTC	1 cut(s)	640
BsoBI	CYCGRG	1 cut(s)	566
Bsp1286I	GDGCHC	1 cut(s)	81
Bsp143I	GATC	2 cut(s)	175, 496
BspFNI	CGCG	1 cut(s)	224
BspHI	TCATGA	1 cut(s)	663
BspLI	GGNNCC	1 cut(s)	142
BspQI	GCTCTTC	1 cut(s)	69
BspTNI	GGTCTC	1 cut(s)	640
BsrDI	GCAATG	2 cut(s)	447, 512
BssMI	GATC	2 cut(s)	175, 496
Bst2UI	CCWGG	1 cut(s)	157
Bst4CI	ACNGT	3 cut(s)	60, 305, 622
Bst6I	CTCTTC	2 cut(s)	69, 94
BstF5I	GGATG	1 cut(s)	165
BstFNI	CGCG	1 cut(s)	224
BstKTI	GATC	2 cut(s)	178, 499
BstMAI	GTCTC	3 cut(s)	475, 481, 640
BstMBI	GATC	2 cut(s)	175, 496
BstMWI	GCNNNNNNNGC	1 cut(s)	221
BstNI	CCWGG	1 cut(s)	157
BstSCI	CCNGG	1 cut(s)	155
BstUI	CGCG	1 cut(s)	224
BstX2I	RGATCY	1 cut(s)	496
BstYI	RGATCY	1 cut(s)	496
BsuI	GTATCC	1 cut(s)	252
BtgZI	GCGATG	1 cut(s)	205
BtsCI	GGATG	1 cut(s)	165
BtsI	GCAGTG	1 cut(s)	181
BtsIMutI	CAGTG	2 cut(s)	181, 618
CciI	TCATGA	1 cut(s)	663
Cfr13I	GGNCC	1 cut(s)	56
CviAII	CATG	3 cut(s)	437, 647, 664
CviJI	RGCY	5 cut(s)	10, 143, 257, 418, 536
CviKI_1	RGCY	5 cut(s)	10, 143, 257, 418, 536
DpnI	GATC	2 cut(s)	177, 498
DpnII	GATC	2 cut(s)	175, 496
Eam1104I	CTCTTC	2 cut(s)	69, 94
EarI	CTCTTC	2 cut(s)	69, 94
Eco31I	GGTCTC	1 cut(s)	640
Eco47I	GGWCC	1 cut(s)	56
Eco88I	CYCGRG	1 cut(s)	566
EcoRII	CCWGG	1 cut(s)	155
Esp3I	CGTCTC	1 cut(s)	475
FaeI	CATG	3 cut(s)	440, 650, 667
FaiI	YATR	9 cut(s)	66, 68, 119, 334, 438, 476, 641, 648, 665
FatI	CATG	3 cut(s)	436, 646, 663
FokI	GGATG	1 cut(s)	172
FspBI	CTAG	2 cut(s)	254, 676
GsuI	CTGGAG	1 cut(s)	118
HapII	CCGG	1 cut(s)	430
Hin1II	CATG	3 cut(s)	440, 650, 667
HindIII	AAGCTT	1 cut(s)	416
HinfI	GANTC	2 cut(s)	233, 667
HpaII	CCGG	1 cut(s)	430
HphI	GGTGA	1 cut(s)	549
Hpy166II	GTNNAC	1 cut(s)	557
Hpy188I	TCNGA	2 cut(s)	469, 496
Hpy188III	TCNNGA	3 cut(s)	135, 272, 664
Hpy8I	GTNNAC	1 cut(s)	557
HpyAV	CCTTC	6 cut(s)	18, 162, 199, 277, 278, 349
HpyCH4III	ACNGT	3 cut(s)	60, 305, 622
HpyCH4V	TGCA	3 cut(s)	215, 280, 650
HpyF10VI	GCNNNNNNNGC	1 cut(s)	221
Hsp92II	CATG	3 cut(s)	440, 650, 667
Kzo9I	GATC	2 cut(s)	175, 496
LguI	GCTCTTC	1 cut(s)	69
LmnI	GCTCC	1 cut(s)	140
LpnPI	CCDG	5 cut(s)	142, 148, 169, 386, 443
MaeI	CTAG	2 cut(s)	254, 676
MaeIII	GTNAC	1 cut(s)	305
MalI	GATC	2 cut(s)	177, 498
MboI	GATC	2 cut(s)	175, 496
MboII	GAAGA	3 cut(s)	81, 86, 228
MfeI	CAATTG	1 cut(s)	15
MflI	RGATCY	1 cut(s)	496
MhlI	GDGCHC	1 cut(s)	81
MluCI	AATT	8 cut(s)	15, 190, 210, 287, 347, 369, 606, 658
MnlI	CCTC	9 cut(s)	131, 159, 320, 531, 534, 537, 540, 562, 575
MseI	TTAA	3 cut(s)	315, 405, 420
MspI	CCGG	1 cut(s)	430
MspR9I	CCNGG	1 cut(s)	157
MunI	CAATTG	1 cut(s)	15
Mva1269I	GAATGC	1 cut(s)	215
MvaI	CCWGG	1 cut(s)	157
MvnI	CGCG	1 cut(s)	224
MwoI	GCNNNNNNNGC	1 cut(s)	221
NdeII	GATC	2 cut(s)	175, 496
NlaIII	CATG	3 cut(s)	440, 650, 667
NlaIV	GGNNCC	1 cut(s)	142
PaeR7I	CTCGAG	1 cut(s)	566
PagI	TCATGA	1 cut(s)	663
PciSI	GCTCTTC	1 cut(s)	69
PcsI	WCGNNNNNNNCGW	2 cut(s)	228, 348
PctI	GAATGC	1 cut(s)	215
PfeI	GAWTC	2 cut(s)	233, 667
PflFI	GACNNNGTC	1 cut(s)	42
PfoI	TCCNGGA	1 cut(s)	155
Psp6I	CCWGG	1 cut(s)	155
PspGI	CCWGG	1 cut(s)	155
PspN4I	GGNNCC	1 cut(s)	142
PspPI	GGNCC	1 cut(s)	56
PspXI	VCTCGAGB	1 cut(s)	566
PsuI	RGATCY	1 cut(s)	496
PsyI	GACNNNGTC	1 cut(s)	42
SapI	GCTCTTC	1 cut(s)	69
SaqAI	TTAA	3 cut(s)	315, 405, 420
Sau3AI	GATC	2 cut(s)	175, 496
Sau96I	GGNCC	1 cut(s)	56
ScrFI	CCNGG	1 cut(s)	157
SduI	GDGCHC	1 cut(s)	81
SetI	ASST	9 cut(s)	12, 259, 270, 312, 405, 420, 538, 573, 595
Sfr274I	CTCGAG	1 cut(s)	566
SinI	GGWCC	1 cut(s)	56
SlaI	CTCGAG	1 cut(s)	566
SmlI	CTYRAG	2 cut(s)	531, 566
SmoI	CTYRAG	2 cut(s)	531, 566
Sse9I	AATT	8 cut(s)	15, 190, 210, 287, 347, 369, 606, 658
SspMI	CTAG	2 cut(s)	254, 676
StyD4I	CCNGG	1 cut(s)	155
TaaI	ACNGT	3 cut(s)	60, 305, 622
TaqI	TCGA	5 cut(s)	174, 300, 351, 413, 567
TasI	AATT	8 cut(s)	15, 190, 210, 287, 347, 369, 606, 658
TfiI	GAWTC	2 cut(s)	233, 667
Tru1I	TTAA	3 cut(s)	315, 405, 420
Tru9I	TTAA	3 cut(s)	315, 405, 420
TscAI	CASTG	2 cut(s)	188, 625
TspDTI	ATGAA	6 cut(s)	83, 87, 106, 208, 531, 652
TspGWI	ACGGA	1 cut(s)	499
TspRI	CASTG	2 cut(s)	188, 625
Tth111I	GACNNNGTC	1 cut(s)	42
VpaK11BI	GGWCC	1 cut(s)	56
XapI	RAATTY	2 cut(s)	287, 347
XhoI	CTCGAG	1 cut(s)	566
XspI	CTAG	2 cut(s)	254, 676

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.