AT5G54770

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
Physical Location & Seq
Forward (+)
22246519 .. 22248332
1814 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54770.1

Sequence Viewer

Length: 1050 bp
ATGGCTGCCATAGCTTCTACTCTCTCTCTTTCTTCAACCAAACCTCAGAGACTTTTCGATTCTTCCTTCCATGGCTCAGCCATCTCCGCAGCTCCTATCTCCATCGGTCTCAAACCACGATCTTTCTCCGTTCGCGCCACAACCGCTGGTTACGACTTGAACGCTTTCACGTTCGACCCGATCAAGGAATCGATCGTGTCACGTGAGATGACGAGAAGGTACATGACGGATATGATCACTTATGCTGAGACTGATGTCGTCGTCGTTGGTGCTGGATCTGCTGGTTTATCTGCTGCTTATGAGATCAGTAAGAACCCTAATGTTCAAGTTGCCATTATTGAGCAATCTGTTAGTCCTGGTGGTGGTGCTTGGCTCGGTGGTCAGCTTTTCTCCGCCATGATTGTTCGCAAACCAGCTCACTTGTTCCTTGACGAGATTGGTGTGGCTTACGATGAGCAAGACACCTACGTTGTGGTTAAGCACGCGGCTCTGTTCACATCAACCATCATGAGCAAGCTTTTGGCTCGTCCTAATGTGAAGCTCTTCAACGCTGTGGCTGCTGAGGATTTGATTGTGAAAGGAAACAGAGTTGGTGGTGTGGTGACTAACTGGGCTCTTGTGGCTCAGAACCACCACACACAATCGTGTATGGACCCTAATGTTATGGAAGCGAAGATTGTGGTTAGCTCTTGTGGTCATGATGGTCCTTTTGGAGCTACTGGTGTTAAGAGGTTGAAGAGTATTGGGATGATTGATCATGTTCCTGGGATGAAAGCTTTGGACATGAACACTGCTGAGGACGCTATTGTGAGGTTGACCAGAGAGGTTGTTCCTGGTATGATTGTTACCGGTATGGAAGTTGCTGAGATCGATGGTGCACCGAGAATGGGACCAACTTTTGGTGCTATGATGATATCGGGACAAAAGGCGGGACAACTTGCTCTTAAGGCTTTGGGGCTACCGAATGCTATTGACGGAACTCTCGTTGGAAACCTAAGCCCGGAGCTGGTCTTAGCCGCAGCAGATTCAGCTGAAACCGTAGATGCTTAA

Protein Analysis

349

Amino Acids

36.66

Weight (kDa)

5.82

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 67 - 300 1e-115 Thi4 family
FAD_binding_2 PF00890 85 - 121 1.5e-06 FAD binding domain
DAO PF01266 85 - 130 4.8e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 88 - 123 3.4e-06 NAD(P)-binding Rossmann-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 899
AccII CGCG 2 cut(s) 135, 485
AciI CCGC 6 cut(s) 87, 144, 393, 485, 929, 1017
AclWI GGATC 1 cut(s) 283
AcvI CACGTG 1 cut(s) 203
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 6 cut(s) 184, 362, 887, 899, 1000, 1006
AflII CTTAAG 1 cut(s) 944
AgeI ACCGGT 1 cut(s) 848
AgsI TTSAA 5 cut(s) 36, 160, 326, 547, 736
AjnI CCWGG 3 cut(s) 355, 763, 832
AleI CACNNNNGTG 1 cut(s) 643
Alw21I GWGCWC 1 cut(s) 880
Alw26I GTCTC 3 cut(s) 43, 113, 242
Alw44I GTGCAC 1 cut(s) 876
AlwI GGATC 1 cut(s) 283
ApaLI GTGCAC 1 cut(s) 876
ApeKI GCWGC 5 cut(s) 5, 89, 293, 557, 1019
AsiGI ACCGGT 1 cut(s) 848
Asp700I GAANNNNTTC 2 cut(s) 164, 542
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 3 cut(s) 652, 704, 890
AsuC2I CCSGG 1 cut(s) 1001
AsuHPI GGTGA 1 cut(s) 613
AvaII GGWCC 3 cut(s) 652, 704, 890
BaeGI GKGCMC 1 cut(s) 880
BanII GRGCYC 1 cut(s) 616
BbrPI CACGTG 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 880
BbvCI CCTCAGC 2 cut(s) 561, 795
BbvI GCAGC 4 cut(s) 101, 280, 544, 1031
BccI CCATC 5 cut(s) 89, 110, 512, 695, 866
BciT130I CCWGG 3 cut(s) 357, 765, 834
BclI TGATCA 2 cut(s) 234, 754
BcnI CCSGG 1 cut(s) 1001
BcoDI GTCTC 3 cut(s) 43, 113, 242
BfrI CTTAAG 1 cut(s) 944
BisI GCNGC 7 cut(s) 6, 90, 294, 486, 558, 1017, 1020
BlpI GCTNAGC 1 cut(s) 76
BlsI GCNGC 7 cut(s) 7, 91, 295, 487, 559, 1018, 1021
Bme1390I CCNGG 4 cut(s) 357, 765, 834, 1001
Bme18I GGWCC 3 cut(s) 652, 704, 890
BmgT120I GGNCC 3 cut(s) 652, 704, 890
BmiI GGNNCC 2 cut(s) 654, 891
BmrFI CCNGG 4 cut(s) 357, 765, 834, 1001
BmrI ACTGGG 1 cut(s) 619
BmsI GCATC 1 cut(s) 1033
BmuI ACTGGG 1 cut(s) 619
BoxI GACNNNNGTC 1 cut(s) 254
Bpu10I CCTNAGC 3 cut(s) 561, 795, 995
Bpu1102I GCTNAGC 1 cut(s) 76
BpuMI CCSGG 1 cut(s) 1001
Bsa29I ATCGAT 2 cut(s) 191, 870
BsaAI YACGTR 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 70, 764
BsaWI WCCGGW 1 cut(s) 848
Bsc4I CCNNNNNNNGG 6 cut(s) 184, 362, 887, 899, 1000, 1006
Bse118I RCCGGY 1 cut(s) 848
Bse1I ACTGG 2 cut(s) 614, 724
BseBI CCWGG 3 cut(s) 357, 765, 834
BseCI ATCGAT 2 cut(s) 191, 870
BseDI CCNNGG 2 cut(s) 70, 764
BseGI GGATG 2 cut(s) 753, 774
BseLI CCNNNNNNNGG 6 cut(s) 184, 362, 887, 899, 1000, 1006
BseMII CTCAG 7 cut(s) 59, 90, 237, 552, 638, 786, 855
BseNI ACTGG 2 cut(s) 614, 724
BseSI GKGCMC 1 cut(s) 880
BseXI GCAGC 4 cut(s) 101, 280, 544, 1031
Bsh1236I CGCG 2 cut(s) 135, 485
Bsh1285I CGRYCG 1 cut(s) 195
BshTI ACCGGT 1 cut(s) 848
BshVI ATCGAT 2 cut(s) 191, 870
BsiEI CGRYCG 1 cut(s) 195
BsiHKAI GWGCWC 1 cut(s) 880
BsiSI CCGG 2 cut(s) 849, 1001
BslFI GGGAC 3 cut(s) 903, 933, 945
BslI CCNNNNNNNGG 6 cut(s) 184, 362, 887, 899, 1000, 1006
BsmAI GTCTC 3 cut(s) 43, 113, 242
BsmFI GGGAC 3 cut(s) 903, 933, 945
BsmI GAATGC 1 cut(s) 970
Bso31I GGTCTC 1 cut(s) 113
Bsp1286I GDGCHC 2 cut(s) 616, 880
Bsp143I GATC 8 cut(s) 119, 180, 192, 234, 275, 303, 754, 867
Bsp1720I GCTNAGC 1 cut(s) 76
Bsp19I CCATGG 1 cut(s) 70
BspACI CCGC 6 cut(s) 87, 144, 393, 485, 929, 1017
BspCNI CTCAG 7 cut(s) 58, 89, 238, 553, 637, 787, 856
BspDI ATCGAT 2 cut(s) 191, 870
BspFNI CGCG 2 cut(s) 135, 485
BspHI TCATGA 2 cut(s) 507, 697
BspLI GGNNCC 2 cut(s) 654, 891
BspPI GGATC 1 cut(s) 283
BspQI GCTCTTC 1 cut(s) 548
BspTI CTTAAG 1 cut(s) 944
BspTNI GGTCTC 1 cut(s) 113
BsrFI RCCGGY 1 cut(s) 848
BsrI ACTGG 2 cut(s) 614, 724
BssAI RCCGGY 1 cut(s) 848
BssECI CCNNGG 2 cut(s) 70, 764
BssMI GATC 8 cut(s) 119, 180, 192, 234, 275, 303, 754, 867
BssT1I CCWWGG 1 cut(s) 70
Bst2UI CCWGG 3 cut(s) 357, 765, 834
Bst4CI ACNGT 1 cut(s) 1039
Bst6I CTCTTC 2 cut(s) 548, 731
BstAFI CTTAAG 1 cut(s) 944
BstBAI YACGTR 1 cut(s) 203
BstC8I GCNNGC 2 cut(s) 483, 515
BstDEI CTNAG 9 cut(s) 45, 76, 246, 561, 624, 795, 864, 995, 1012
BstDSI CCRYGG 1 cut(s) 70
BstF5I GGATG 2 cut(s) 753, 774
BstFNI CGCG 2 cut(s) 135, 485
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 8 cut(s) 122, 183, 195, 237, 278, 306, 757, 870
BstMAI GTCTC 3 cut(s) 43, 113, 242
BstMBI GATC 8 cut(s) 119, 180, 192, 234, 275, 303, 754, 867
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 9 cut(s) 11, 86, 143, 278, 557, 620, 800, 947, 1028
BstNI CCWGG 3 cut(s) 357, 765, 834
BstPAI GACNNNNGTC 1 cut(s) 254
BstSCI CCNGG 4 cut(s) 355, 763, 832, 999
BstSLI GKGCMC 1 cut(s) 880
BstUI CGCG 2 cut(s) 135, 485
BstV1I GCAGC 4 cut(s) 101, 280, 544, 1031
BstX2I RGATCY 1 cut(s) 275
BstYI RGATCY 1 cut(s) 275
Bsu15I ATCGAT 2 cut(s) 191, 870
BsuTUI ATCGAT 2 cut(s) 191, 870
BtgI CCRYGG 1 cut(s) 70
BtsCI GGATG 2 cut(s) 753, 774
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 1 cut(s) 789
Cac8I GCNNGC 2 cut(s) 483, 515
CciI TCATGA 2 cut(s) 507, 697
CfoI GCGC 1 cut(s) 137
Cfr10I RCCGGY 1 cut(s) 848
Cfr13I GGNCC 3 cut(s) 652, 704, 890
ClaI ATCGAT 2 cut(s) 191, 870
CseI GACGC 1 cut(s) 809
Csp6I GTAC 1 cut(s) 220
CspAI ACCGGT 1 cut(s) 848
CviAII CATG 7 cut(s) 71, 223, 397, 508, 698, 758, 784
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 9 cut(s) 45, 76, 246, 561, 624, 795, 864, 995, 1012
DpnI GATC 8 cut(s) 121, 182, 194, 236, 277, 305, 756, 869
DpnII GATC 8 cut(s) 119, 180, 192, 234, 275, 303, 754, 867
Eam1104I CTCTTC 2 cut(s) 548, 731
EarI CTCTTC 2 cut(s) 548, 731
EciI GGCGGA 1 cut(s) 382
Eco130I CCWWGG 1 cut(s) 70
Eco24I GRGCYC 1 cut(s) 616
Eco31I GGTCTC 1 cut(s) 113
Eco32I GATATC 1 cut(s) 915
Eco47I GGWCC 3 cut(s) 652, 704, 890
Eco72I CACGTG 1 cut(s) 203
EcoRII CCWGG 3 cut(s) 355, 763, 832
EcoRV GATATC 1 cut(s) 915
EcoT14I CCWWGG 1 cut(s) 70
EcoT38I GRGCYC 1 cut(s) 616
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 7 cut(s) 74, 226, 400, 511, 701, 761, 787
FaqI GGGAC 3 cut(s) 903, 933, 945
FatI CATG 7 cut(s) 70, 222, 396, 507, 697, 757, 783
FauI CCCGC 1 cut(s) 922
FbaI TGATCA 2 cut(s) 234, 754
Fnu4HI GCNGC 7 cut(s) 6, 90, 294, 486, 558, 1017, 1020
FokI GGATG 2 cut(s) 760, 781
FriOI GRGCYC 1 cut(s) 616
Fsp4HI GCNGC 7 cut(s) 6, 90, 294, 486, 558, 1017, 1020
GlaI GCGC 1 cut(s) 136
GluI GCNGC 7 cut(s) 6, 90, 294, 486, 558, 1017, 1020
HapII CCGG 2 cut(s) 849, 1001
HgaI GACGC 1 cut(s) 809
HhaI GCGC 1 cut(s) 137
Hin1II CATG 7 cut(s) 74, 226, 400, 511, 701, 761, 787
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HincII GTYRAC 1 cut(s) 816
HindII GTYRAC 1 cut(s) 816
HindIII AAGCTT 2 cut(s) 515, 774
HinfI GANTC 3 cut(s) 59, 188, 1025
HpaII CCGG 2 cut(s) 849, 1001
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 3 cut(s) 495, 816, 878
Hpy188I TCNGA 2 cut(s) 48, 627
Hpy188III TCNNGA 3 cut(s) 508, 698, 918
Hpy8I GTNNAC 3 cut(s) 495, 816, 878
Hpy99I CGWCG 2 cut(s) 263, 266
HpyAV CCTTC 2 cut(s) 76, 210
HpyCH4III ACNGT 1 cut(s) 1039
HpyCH4IV ACGT 3 cut(s) 170, 202, 468
HpyCH4V TGCA 1 cut(s) 878
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 86, 143, 278, 557, 620, 800, 947, 1028
HpyF3I CTNAG 9 cut(s) 45, 76, 246, 561, 624, 795, 864, 995, 1012
HpySE526I ACGT 3 cut(s) 170, 202, 468
Hsp92II CATG 7 cut(s) 74, 226, 400, 511, 701, 761, 787
HspAI GCGC 1 cut(s) 135
Ksp22I TGATCA 2 cut(s) 234, 754
Kzo9I GATC 8 cut(s) 119, 180, 192, 234, 275, 303, 754, 867
LguI GCTCTTC 1 cut(s) 548
LmnI GCTCC 3 cut(s) 97, 713, 1003
Lsp1109I GCAGC 4 cut(s) 101, 280, 544, 1031
LweI GCATC 1 cut(s) 1033
MaeII ACGT 3 cut(s) 170, 202, 468
MaeIII GTNAC 4 cut(s) 149, 198, 601, 844
MalI GATC 8 cut(s) 121, 182, 194, 236, 277, 305, 756, 869
MboI GATC 8 cut(s) 119, 180, 192, 234, 275, 303, 754, 867
MboII GAAGA 5 cut(s) 24, 54, 535, 685, 748
MflI RGATCY 1 cut(s) 275
MhlI GDGCHC 2 cut(s) 616, 880
MmeI TCCRAC 1 cut(s) 967
MnlI CCTC 6 cut(s) 54, 556, 723, 790, 804, 817
MroXI GAANNNNTTC 2 cut(s) 164, 542
MseI TTAA 4 cut(s) 477, 726, 945, 1048
MslI CAYNNNNRTG 1 cut(s) 643
MspA1I CMGCKG 2 cut(s) 146, 1031
MspCI CTTAAG 1 cut(s) 944
MspI CCGG 2 cut(s) 849, 1001
MspR9I CCNGG 4 cut(s) 357, 765, 834, 1001
Mva1269I GAATGC 1 cut(s) 970
MvaI CCWGG 3 cut(s) 357, 765, 834
MvnI CGCG 2 cut(s) 135, 485
MwoI GCNNNNNNNGC 9 cut(s) 11, 86, 143, 278, 557, 620, 800, 947, 1028
NciI CCSGG 1 cut(s) 1001
NcoI CCATGG 1 cut(s) 70
NdeII GATC 8 cut(s) 119, 180, 192, 234, 275, 303, 754, 867
NlaIII CATG 7 cut(s) 74, 226, 400, 511, 701, 761, 787
NlaIV GGNNCC 2 cut(s) 654, 891
NmuCI GTSAC 2 cut(s) 198, 601
OliI CACNNNNGTG 1 cut(s) 643
PagI TCATGA 2 cut(s) 507, 697
PciSI GCTCTTC 1 cut(s) 548
PcsI WCGNNNNNNNCGW 2 cut(s) 176, 981
PctI GAATGC 1 cut(s) 970
PdmI GAANNNNTTC 2 cut(s) 164, 542
PfeI GAWTC 3 cut(s) 59, 188, 1025
PflMI CCANNNNNTGG 1 cut(s) 899
PinAI ACCGGT 1 cut(s) 848
PkrI GCNGC 7 cut(s) 7, 91, 295, 487, 559, 1018, 1021
Ple19I CGATCG 1 cut(s) 195
PmaCI CACGTG 1 cut(s) 203
PmlI CACGTG 1 cut(s) 203
Ppu21I YACGTR 1 cut(s) 203
PshAI GACNNNNGTC 1 cut(s) 254
Psp6I CCWGG 3 cut(s) 355, 763, 832
PspCI CACGTG 1 cut(s) 203
PspGI CCWGG 3 cut(s) 355, 763, 832
PspN4I GGNNCC 2 cut(s) 654, 891
PspPI GGNCC 3 cut(s) 652, 704, 890
PsuI RGATCY 1 cut(s) 275
PvuI CGATCG 1 cut(s) 195
PvuII CAGCTG 1 cut(s) 1031
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
RseI CAYNNNNRTG 1 cut(s) 643
SapI GCTCTTC 1 cut(s) 548
SaqAI TTAA 4 cut(s) 477, 726, 945, 1048
SatI GCNGC 7 cut(s) 6, 90, 294, 486, 558, 1017, 1020
Sau3AI GATC 8 cut(s) 119, 180, 192, 234, 275, 303, 754, 867
Sau96I GGNCC 3 cut(s) 652, 704, 890
ScrFI CCNGG 4 cut(s) 357, 765, 834, 1001
SduI GDGCHC 2 cut(s) 616, 880
SfaNI GCATC 1 cut(s) 1033
SinI GGWCC 3 cut(s) 652, 704, 890
SmiMI CAYNNNNRTG 1 cut(s) 643
SmlI CTYRAG 1 cut(s) 944
SmoI CTYRAG 1 cut(s) 944
SsiI CCGC 6 cut(s) 87, 144, 393, 485, 929, 1017
StyD4I CCNGG 4 cut(s) 355, 763, 832, 999
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 1 cut(s) 1039
TaiI ACGT 3 cut(s) 173, 205, 471
TaqI TCGA 4 cut(s) 57, 174, 191, 870
TaqII GACCGA 1 cut(s) 95
TauI GCSGC 2 cut(s) 488, 1019
TfiI GAWTC 3 cut(s) 59, 188, 1025
Tru1I TTAA 4 cut(s) 477, 726, 945, 1048
Tru9I TTAA 4 cut(s) 477, 726, 945, 1048
TscAI CASTG 1 cut(s) 796
TseFI GTSAC 2 cut(s) 198, 601
TseI GCWGC 5 cut(s) 5, 89, 293, 557, 1019
Tsp45I GTSAC 2 cut(s) 198, 601
TspDTI ATGAA 2 cut(s) 785, 800
TspGWI ACGGA 3 cut(s) 118, 242, 990
TspRI CASTG 1 cut(s) 796
Van91I CCANNNNNTGG 1 cut(s) 899
Vha464I CTTAAG 1 cut(s) 944
VneI GTGCAC 1 cut(s) 876
VpaK11BI GGWCC 3 cut(s) 652, 704, 890
XmnI GAANNNNTTC 2 cut(s) 164, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.